Effective enrichment of trace exosomes for the label-free SERS detection via low-cost thermophoretic profiling
Biosens Bioelectron. 2024 Feb 23;253:116164. doi: 10.1016/j.bios.2024.116164. Online ahead of print.ABSTRACTExosome-based liquid biopsies possess great potential in monitoring cancer development However, current exosome detection biosensors require large exosome volumes, showing the weak detection sensitivity. Besides, these methods pay little attention to in situ analysis of exosomes, hence limiting the provision of more accurate clinically-relevant information. Herein, we develop an innovative label-free biosensor combining the low-cost thermophoretic enrichment method with the surface-enhanced Raman spectroscopy (SERS) ...
Source: Biosensors and Bioelectronics - February 29, 2024 Category: Biotechnology Authors: Yu Guo Ruiyuan Zhang Hongjun You Jixiang Fang Source Type: research
Ultra-conformal epidermal antenna for multifunctional motion artifact-free sensing and point-of-care monitoring
Biosens Bioelectron. 2024 Feb 24;253:116150. doi: 10.1016/j.bios.2024.116150. Online ahead of print.ABSTRACTAccurate acquisition of physiological and physical information from human tissue is essential for health monitoring, disease prevention and treatment. The existing antennas with traditional rigid or flexible substrates are susceptible to motion artifacts in wearable applications due to the miniaturization limitation and lack of proper adhesion and conformal interfaces with the skin. Recent advances in wearable radio frequency (RF) bioelectronics directly drawn on the skin are a promising solution for future skin-inte...
Source: Biosensors and Bioelectronics - February 29, 2024 Category: Biotechnology Authors: Zengxiang Wang Xia Xiao Wenqi Wu Xiaofeng Zhang Yanwei Pang Source Type: research
Digital monitoring of the microchannel filling flow dynamics using a non-contactless smartphone-based nano-liter precision flow velocity meter
Biosens Bioelectron. 2024 Feb 13;252:116130. doi: 10.1016/j.bios.2024.116130. Online ahead of print.ABSTRACTMicrofluidic systems find widespread applications in diagnostics, biological research, chemistry, and engineering studies. Among their many critical parameters, flow rate plays a pivotal role in maintaining the functionality of microfluidic systems, including droplet-based microfluidic devices and those used in cell culture. It also significantly influences microfluidic mixing processes. Although various flow rate measurement devices have been developed, the challenge remains in accurately measuring flow rates within...
Source: Biosensors and Bioelectronics - February 28, 2024 Category: Biotechnology Authors: Weiming Xu Abdulkadir Yasin Atik Levent Beker Hatice Ceylan Koydemir Source Type: research
Sea hedgehog-inspired surface-enhanced Raman scattering biosensor probe for ultrasensitive determination of Staphylococcus aureus in food supplements
Biosens Bioelectron. 2024 Feb 23;252:116146. doi: 10.1016/j.bios.2024.116146. Online ahead of print.ABSTRACTStaphylococcus aureus contamination in food supplements poses substantial challenges to public health and large-scale production but the sensitive detection in a timely manner remains a bottleneck. Drawing inspiration from the sea hedgehog, gold nanostars (AuNSs) were leveraged to design an ultrasensitive surface-enhanced Raman scattering (SERS) biosensor for the determination of Staphylococcus aureus in food supplements. Besides the surface enhancement furnished by the AuNSs, Raman reporter molecules and specific ap...
Source: Biosensors and Bioelectronics - February 28, 2024 Category: Biotechnology Authors: Yi Tao Qing Liu Ningtao Cheng Source Type: research
MoO < sub > 3 < /sub > /MIL-125-NH < sub > 2 < /sub > with boosted peroxidase-like activity for electrochemical staphylococcus aureus sensing via specific recognition of bacteriophages
Biosens Bioelectron. 2024 Feb 16;252:116134. doi: 10.1016/j.bios.2024.116134. Online ahead of print.ABSTRACTHerein, novel nanozyme mimics MoO3/MIL-125-NH2 were reported and conjugated with bacteriophages as a new electrochemical probe for high sensitivity and specific electrochemical detection of staphylococcus aureus. The excellent peroxidase-like activity of MoO3/MIL-125-NH2 composites was attributed to the integration of MIL-125-NH2 with MoO3, which can boost the generation of superoxide radicals (O• 2-) and thus promote the oxidation of TMB in the presence of H2O2. In this work, two bacteriophages named SapYZU04 and ...
Source: Biosensors and Bioelectronics - February 28, 2024 Category: Biotechnology Authors: Yajun Gao Suhui Xu Ge Guo Yajie Li Wenyuan Zhou Huaxiang Li Zhenquan Yang Source Type: research
Digital monitoring of the microchannel filling flow dynamics using a non-contactless smartphone-based nano-liter precision flow velocity meter
Biosens Bioelectron. 2024 Feb 13;252:116130. doi: 10.1016/j.bios.2024.116130. Online ahead of print.ABSTRACTMicrofluidic systems find widespread applications in diagnostics, biological research, chemistry, and engineering studies. Among their many critical parameters, flow rate plays a pivotal role in maintaining the functionality of microfluidic systems, including droplet-based microfluidic devices and those used in cell culture. It also significantly influences microfluidic mixing processes. Although various flow rate measurement devices have been developed, the challenge remains in accurately measuring flow rates within...
Source: Biosensors and Bioelectronics - February 28, 2024 Category: Biotechnology Authors: Weiming Xu Abdulkadir Yasin Atik Levent Beker Hatice Ceylan Koydemir Source Type: research
Sea hedgehog-inspired surface-enhanced Raman scattering biosensor probe for ultrasensitive determination of Staphylococcus aureus in food supplements
Biosens Bioelectron. 2024 Feb 23;252:116146. doi: 10.1016/j.bios.2024.116146. Online ahead of print.ABSTRACTStaphylococcus aureus contamination in food supplements poses substantial challenges to public health and large-scale production but the sensitive detection in a timely manner remains a bottleneck. Drawing inspiration from the sea hedgehog, gold nanostars (AuNSs) were leveraged to design an ultrasensitive surface-enhanced Raman scattering (SERS) biosensor for the determination of Staphylococcus aureus in food supplements. Besides the surface enhancement furnished by the AuNSs, Raman reporter molecules and specific ap...
Source: Biosensors and Bioelectronics - February 28, 2024 Category: Biotechnology Authors: Yi Tao Qing Liu Ningtao Cheng Source Type: research
MoO < sub > 3 < /sub > /MIL-125-NH < sub > 2 < /sub > with boosted peroxidase-like activity for electrochemical staphylococcus aureus sensing via specific recognition of bacteriophages
Biosens Bioelectron. 2024 Feb 16;252:116134. doi: 10.1016/j.bios.2024.116134. Online ahead of print.ABSTRACTHerein, novel nanozyme mimics MoO3/MIL-125-NH2 were reported and conjugated with bacteriophages as a new electrochemical probe for high sensitivity and specific electrochemical detection of staphylococcus aureus. The excellent peroxidase-like activity of MoO3/MIL-125-NH2 composites was attributed to the integration of MIL-125-NH2 with MoO3, which can boost the generation of superoxide radicals (O• 2-) and thus promote the oxidation of TMB in the presence of H2O2. In this work, two bacteriophages named SapYZU04 and ...
Source: Biosensors and Bioelectronics - February 28, 2024 Category: Biotechnology Authors: Yajun Gao Suhui Xu Ge Guo Yajie Li Wenyuan Zhou Huaxiang Li Zhenquan Yang Source Type: research
Enhanced selective discrimination of point-mutated viral RNA through false amplification regulatory direct insertion in rolling circle amplification
In this study, the limit of detection was 400 aM for viral RNA, and we successfully identified a dominant SNP variant selectively. Clinical tests were also conducted.PMID:38412685 | DOI:10.1016/j.bios.2024.116145 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - February 27, 2024 Category: Biotechnology Authors: Hakbeom Lee Juneseok You Hansol Lee Woojoo Kim Kuewhan Jang Jinsung Park Sungsoo Na Source Type: research
Microfabrication of engineered Lactococcus lactis biocarriers with genetically programmed immunorecognition probes for sensitive lateral flow immunoassay of antibiotic in milk and lake water
Biosens Bioelectron. 2024 Feb 19;252:116139. doi: 10.1016/j.bios.2024.116139. Online ahead of print.ABSTRACTMicro/nanomaterials display considerable potential for increasing the sensitivity of lateral flow immunoassay (LFIA) by acting as 3D carriers for both antibodies and signals. The key to achieving high detection sensitivity depends on the probe's orientation on the material surface and its multivalent biomolecular interactions with targets. Here, we engineer Lactococcus lactis as the bacterial microcarrier (BMC) for a multivalent immunorecognition probe that was genetically programmed to display multifunctional compon...
Source: Biosensors and Bioelectronics - February 27, 2024 Category: Biotechnology Authors: Fuyuan Zhang Jiajie Chen Fangkun Zhao Minxuan Liu Kaige Peng Yuanhao Pu Yaxin Sang Shuo Wang Xianghong Wang Source Type: research
Two Birds with one Stone: Dual-mode immunoassay constructed using a novel emitter ethylene glycol-induced perylene diimide and a multifunctional ANS probe
In this study, ethylene glycol (EG) was used to induce the self-assembly of PDI supramolecules for the preparation of ultrathin EG-PDI nanosheets characterized by low crystallinity and weak stacking interaction. Notably, EG-PDI integrates luminescent and catalytic functions into one device, accelerating the interfacial electron transfer and the faster charge transfer kinetics of EG-PDI with K2S2O8. Furthermore, the narrow band gap of EG-PDI facilitates its excitation at an ultra-low potential (-0.3 V). To improve the efficiency of tumor marker analysis, multifunctional Au nanostars (ANS) was introduced both as an energy ac...
Source: Biosensors and Bioelectronics - February 25, 2024 Category: Biotechnology Authors: Wenjing Lai Shijie Yan Mingzhe Jiang Zhina Li Min Wang Chulei Zhao Chaoyun Ma Zhong Wei Chenglin Hong Source Type: research
Two Birds with one Stone: Dual-mode immunoassay constructed using a novel emitter ethylene glycol-induced perylene diimide and a multifunctional ANS probe
In this study, ethylene glycol (EG) was used to induce the self-assembly of PDI supramolecules for the preparation of ultrathin EG-PDI nanosheets characterized by low crystallinity and weak stacking interaction. Notably, EG-PDI integrates luminescent and catalytic functions into one device, accelerating the interfacial electron transfer and the faster charge transfer kinetics of EG-PDI with K2S2O8. Furthermore, the narrow band gap of EG-PDI facilitates its excitation at an ultra-low potential (-0.3 V). To improve the efficiency of tumor marker analysis, multifunctional Au nanostars (ANS) was introduced both as an energy ac...
Source: Biosensors and Bioelectronics - February 25, 2024 Category: Biotechnology Authors: Wenjing Lai Shijie Yan Mingzhe Jiang Zhina Li Min Wang Chulei Zhao Chaoyun Ma Zhong Wei Chenglin Hong Source Type: research
Two Birds with one Stone: Dual-mode immunoassay constructed using a novel emitter ethylene glycol-induced perylene diimide and a multifunctional ANS probe
In this study, ethylene glycol (EG) was used to induce the self-assembly of PDI supramolecules for the preparation of ultrathin EG-PDI nanosheets characterized by low crystallinity and weak stacking interaction. Notably, EG-PDI integrates luminescent and catalytic functions into one device, accelerating the interfacial electron transfer and the faster charge transfer kinetics of EG-PDI with K2S2O8. Furthermore, the narrow band gap of EG-PDI facilitates its excitation at an ultra-low potential (-0.3 V). To improve the efficiency of tumor marker analysis, multifunctional Au nanostars (ANS) was introduced both as an energy ac...
Source: Biosensors and Bioelectronics - February 25, 2024 Category: Biotechnology Authors: Wenjing Lai Shijie Yan Mingzhe Jiang Zhina Li Min Wang Chulei Zhao Chaoyun Ma Zhong Wei Chenglin Hong Source Type: research
Sensing of gene expression in live cells using electrical impedance spectroscopy and DNA-functionalized gold nanoparticles
Biosens Bioelectron. 2024 Jan 16;252:116041. doi: 10.1016/j.bios.2024.116041. Online ahead of print.ABSTRACTA novel electrical impedance spectroscopy-based method for non-destructive sensing of gene expression in living cells is presented. The approach used takes advantage of the robustness and responsiveness of electrical impedance spectroscopy and the highly specific and selective nature of DNA hybridization. The technique uses electrical impedance spectroscopy and gold nanoparticles functionalized with single-stranded DNA complementary to an mRNA of interest to provide reliable, real-time, and quantifiable data on gene ...
Source: Biosensors and Bioelectronics - February 24, 2024 Category: Biotechnology Authors: Kian Kadan-Jamal Aakash Jog Marios Sophocleous Tali Dotan Polina Frumin Tamar Kuperberg Goshen Silvia Schuster Adi Avni Yosi Shacham-Diamand Source Type: research
Capacitive immunosensing at gold nanoparticle-decorated reduced graphene oxide electrodes fabricated by one-step laser nanostructuration
This study introduces a capacitive biosensor that leverages laser-engraved reduced graphene oxide (rGO) electrodes decorated with gold nanoparticles (AuNPs). The fabrication involves laser-scribed GO-Au3+ films, yielding rGO-AuNP electrodes, seamlessly transferred onto a PET substrate via a press-stamping methodology. These electrodes have a remarkable affinity for biomolecular recognition after being functionalized with specific bioreceptors. For example, initial studies with human IgG antibodies confirm the detection capabilities of the biosensor using electrochemical capacitance spectroscopy. Furthermore, the biosensor ...
Source: Biosensors and Bioelectronics - February 24, 2024 Category: Biotechnology Authors: Danilo Echeverri Enric Calucho Jose Marrugo-Ram írez Rusl án Álvarez-Diduk Jahir Orozco Arben Merko çi Source Type: research
