Optical fiber biosensors toward in vivo detection
Biosens Bioelectron. 2024 Feb 1;251:116088. doi: 10.1016/j.bios.2024.116088. Online ahead of print.ABSTRACTThis review takes stock of the various optical fiber-based biosensors that could be used for in vivo applications. We discuss the characteristics that biosensors must have to be suitable for such applications and the corresponding transduction modes. In particular, we focus on optical fiber biosensors based on fluorescence, evanescent wave, plasmonics, interferometry, and Raman phenomenon. The operational principles, implemented solutions, and performances are described and debated. The different sensing configuration...
Source: Biosensors and Bioelectronics - February 9, 2024 Category: Biotechnology Authors: Oleksii Bratash Arnaud Buhot Lo ïc Leroy Elodie Engel Source Type: research
Determination of the interior pH of lipid nanoparticles using a pH-sensitive fluorescent dye-based DNA probe
Biosens Bioelectron. 2024 Jan 24;251:116065. doi: 10.1016/j.bios.2024.116065. Online ahead of print.ABSTRACTLipid nanoparticles (LNPs) containing ionizable cationic lipids are proven delivery systems for therapeutic nucleic acids, such as small interfering RNA (siRNA). It is important to understand the relationship between the interior pH of LNPs and the pH of the external environment to understand LNP formulation and function. Here, we developed a simple and rapid approach for determining the pH of the LNP core using a pH-sensitive fluorescent dye-based DNA probe. LNP siRNA systems containing pH-responsive DNA probes (LNP...
Source: Biosensors and Bioelectronics - February 8, 2024 Category: Biotechnology Authors: Bin Zhao Albert Kamanzi Yao Zhang Karen Y T Chan Madelaine Robertson Sabrina Leslie Pieter R Cullis Source Type: research
Detection of Naturally occurring abortive transcripts by Base-Stacking Hybridization Assisted Ligation and PCR amplification
This study offers opportunities to study ATs and other ultra-short oligonucleotides in biological samples.PMID:38330773 | DOI:10.1016/j.bios.2024.116099 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - February 8, 2024 Category: Biotechnology Authors: Shaowei Qin Haizhu Wu Cailin Li Jiarui Yang Weiwei Yan Zhigui He Xuekun Xing Jiayang Zhang Xianglin Xu Lifeng Zhao Xin Su Source Type: research
An IoT-based aptasensor biochip for the diagnosis of periodontal disease
Biosens Bioelectron. 2024 Feb 1;251:116097. doi: 10.1016/j.bios.2024.116097. Online ahead of print.ABSTRACTSevere periodontitis affects nearly 1 billion individuals worldwide, highlighting the need for early diagnosis. Here, an integrated system consisting of a microfluidic chip and a portable point-of-care (POC) diagnostic device is developed using a polymethyl methacrylate (PMMA) chip fabrication and a three-dimensional printing technique, which is automatically controlled by a custom-designed smartphone application to routinely assess the presence of a specific periodontitis biomarker, odontogenic ameloblast-associated ...
Source: Biosensors and Bioelectronics - February 8, 2024 Category: Biotechnology Authors: Thi Thanh-Qui Nguyen Eun-Mi Lee Thi Thanh-Thao Dang Eun Ryung Kim Youngkyung Ko Man Bock Gu Source Type: research
pH-triggered hydrophility-adjustable fluorescent probes for simultaneously imaging lipid droplets and lysosomes and the application in fatty liver detection
Biosens Bioelectron. 2024 Feb 1;251:116084. doi: 10.1016/j.bios.2024.116084. Online ahead of print.ABSTRACTTo study the collaboration between lipid droplets (LDs) and lysosomes, and the lipid change in nonalcoholic fatty liver disease (NAFLD), herein two pH-triggered hydrophility-adjustable fluorescent probes (LD-Lyso and LD-Lyso 1) are designed. The mechanism is based on cyclization and ring-opening with thorough consideration of pH and hydrophilic differences between LDs and lysosomes. Both of the two probes exist in ring-opening form and emit red fluorescence in acidic environment, while they exist in cyclized form and ...
Source: Biosensors and Bioelectronics - February 8, 2024 Category: Biotechnology Authors: Shining Wu Xuechen Li Mingyang Zhou Yuezhi Cui Wenli Wu Jiantao Ping Xuezu Guo Qiongzheng Hu Source Type: research
