Near-infrared electrochemiluminescence biosensors facilitated by thermally activated delayed fluorescence (TADF) emitters for ctDNA analysis
This study highlights the immense potential of TADF emitters in enhancing ECL efficiency and extends the emission wavelength of organic nanomaterials to the NIR region, thereby expanding their applications in biological analysis.PMID:38382269 | DOI:10.1016/j.bios.2024.116103 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - February 21, 2024 Category: Biotechnology Authors: Jinxia Liu Luxia Yang Shijie Li Ke Zhang Xiaobo Zhou Guo Li Li Wu Yuling Qin Source Type: research
Allosteric strand displacement isothermal amplification for the visual detection of Toxoplasma gondii in 30 minutes
This study established a simple, cost-effective, and rapid colorimetric detection method for T. gondii. The entire testing process, from sample collection to results, takes only 0.5 h. These characteristics fulfill the demands of researchers seeking rapid target detection with minimal equipment reliance. For genomic extraction, this study evaluated the ability of two filter papers to capture genomes. A rapid genomic extraction device combined with the two filter papers was designed to simplify the genomic extraction process, which was completed within 10 min and increased the detection sensitivity tenfold. The method utili...
Source: Biosensors and Bioelectronics - February 21, 2024 Category: Biotechnology Authors: Lei Wang Zhaoxuan Zhang Yanger Liu Shenghao Lin Wenqiang Zhang Longjiao Zhu Wentao Xu Yulan Dong Source Type: research
DNAzyme-based ultrasensitive immunoassay: Recent advances and emerging trends
Biosens Bioelectron. 2024 Feb 9;251:116122. doi: 10.1016/j.bios.2024.116122. Online ahead of print.ABSTRACTImmunoassay, as the most commonly used method for protein detection, is simple to operate and highly specific. Sensitivity improvement is always the thrust of immunoassays, especially for the detection of trace quantities. The emergence of artificial enzyme, i.e., DNAzyme, provides a novel approach to improve the detection sensitivity of immunoassay. Simultaneously, its advantages of simple synthesis and high stability enable low cost, broad applicability and long shelf life for immunoassay. In this review, we summari...
Source: Biosensors and Bioelectronics - February 21, 2024 Category: Biotechnology Authors: Meng Wang Zhe Liu Chang Liu Wanghong He Dui Qin Minli You Source Type: research
A trivalent aptasensor by using DNA tetrahedron as scaffold for label-free determination of antibiotics
Biosens Bioelectron. 2024 Feb 16;251:116127. doi: 10.1016/j.bios.2024.116127. Online ahead of print.ABSTRACTOwing to advantage in high sensitivity and fast response, aptamer based electrochemical biosensors have attracted much more attention. However, inappropriate interfacial engineering strategy leads to poor recognition performance, which ascribe to the following factors of immobilized oligonucleotide strand including steric hindrance, interchain entanglement, and unfavorable conformation. In this work, we proposed a DNA tetrahedron based diblock aptamer immobilized strategy for the construction of label-free electroche...
Source: Biosensors and Bioelectronics - February 21, 2024 Category: Biotechnology Authors: Tai Ye Yimin Xu Haohao Chen Min Yuan Hui Cao Liling Hao Xiuxiu Wu Fengqin Yin Fei Xu Source Type: research
Development of a glycated albumin sensor employing dual aptamer-based extended gate field effect transistors
Biosens Bioelectron. 2024 Feb 8;251:116118. doi: 10.1016/j.bios.2024.116118. Online ahead of print.ABSTRACTGlycated albumin (GA), defined as the percentage of serum albumin glycation, is a mid-term glycemic control marker for diabetes. The concentrations of both glycated human serum albumin (GHSA) and total human serum albumin (HSA) are required to calculate GA. Here, we report the development of a GA sensor employing two albumin aptamers: anti-GHSA aptamer which is specific to GHSA and anti-HSA aptamer which recognizes both glycated and non-glycated HSA. We combine these aptamers with extended gate field effect transistor...
Source: Biosensors and Bioelectronics - February 21, 2024 Category: Biotechnology Authors: Mika Hatada Spyridon Pavlidis Koji Sode Source Type: research
Wireless and battery-free wearable biosensing of riboflavin in sweat for precision nutrition
Biosens Bioelectron. 2024 Feb 17;251:116136. doi: 10.1016/j.bios.2024.116136. Online ahead of print.ABSTRACTNutrition assessment is crucial for dietary guidance and prevention of malnutrition. Recent endeavors in wearable biochemical sensors have enabled real-time, in situ analysis of nutrients in sweat. However, the monitoring of riboflavin, an indispensable vitamin B involved in energy metabolism, remains challenging due to its trace level and variations in the sweat matrix. Herein, we report a wireless, battery-free, and flexible wearable biosensing system for the in situ monitoring of sweat riboflavin. Highly sensitive...
Source: Biosensors and Bioelectronics - February 20, 2024 Category: Biotechnology Authors: Zhenghan Shi Peixue Deng Li-Ang Zhou Meng Jin Feiyue Fang Tao Chen Guang Liu Hao Wen Zijian An Hao Liang Yanli Lu Jun Liu Qingjun Liu Source Type: research
Highly sensitive and selective photoelectrochemical detection of bis(2-ethylhexyl)phthalate on broad-spectrum responsive and interfacial electronic interaction induced p-n BiOI/ZnO nanoarrays heterojunction
Biosens Bioelectron. 2024 Feb 8;251:116121. doi: 10.1016/j.bios.2024.116121. Online ahead of print.ABSTRACTBis(2-ethylhexyl)phthalate (DEHP), an endocrine disruptor, shows carcinogenic, teratogenic, mutagenic and estrogenic effects. It is easy to release from plastic materials and migrate to soil environment, causing serious pollution and posing a great threat to human health. In our work, a photoelectrochemical (PEC) sensing platform for DEHP detection was constructed using BiOI/ZnO nanoarrays (NRs) as the transducer species and the DEHP aptamers as the biological recognition elements. ZnO NRs with three-dimensional and l...
Source: Biosensors and Bioelectronics - February 19, 2024 Category: Biotechnology Authors: Xiangle Meng Ao Huang Yuye Li Xiuxiu Dong Tianyan You Source Type: research
Highly sensitive and selective photoelectrochemical detection of bis(2-ethylhexyl)phthalate on broad-spectrum responsive and interfacial electronic interaction induced p-n BiOI/ZnO nanoarrays heterojunction
Biosens Bioelectron. 2024 Feb 8;251:116121. doi: 10.1016/j.bios.2024.116121. Online ahead of print.ABSTRACTBis(2-ethylhexyl)phthalate (DEHP), an endocrine disruptor, shows carcinogenic, teratogenic, mutagenic and estrogenic effects. It is easy to release from plastic materials and migrate to soil environment, causing serious pollution and posing a great threat to human health. In our work, a photoelectrochemical (PEC) sensing platform for DEHP detection was constructed using BiOI/ZnO nanoarrays (NRs) as the transducer species and the DEHP aptamers as the biological recognition elements. ZnO NRs with three-dimensional and l...
Source: Biosensors and Bioelectronics - February 19, 2024 Category: Biotechnology Authors: Xiangle Meng Ao Huang Yuye Li Xiuxiu Dong Tianyan You Source Type: research
Molecularly imprinted composite-based biosensor for the determination of SARS-CoV-2 nucleocapsid protein
This article aims to present a comparative study of three polypyrrole-based molecularly imprinted polymer (MIP) systems for the detection of the recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (rN). The rN is known for its relatively low propensity to mutate compared to other SARS-CoV-2 antigens. The aforementioned systems include screen-printed carbon electrodes (SPCE) modified with gold nanostructures (MIP1), platinum nanostructures (MIP2), and the unmodified SPCE (MIP3), which was used for control. Pulsed amperometric detection (PAD) was employed as the detection technique, ...
Source: Biosensors and Bioelectronics - February 18, 2024 Category: Biotechnology Authors: Maryia Drobysh Vilma Ratautaite Ernestas Brazys Almira Ramanaviciene Arunas Ramanavicius Source Type: research
Aptamer-bivalent-cholesterol-mediated proximity entropy-driven exosomal protein reporter for tumor diagnosis
Biosens Bioelectron. 2024 Feb 6;251:116104. doi: 10.1016/j.bios.2024.116104. Online ahead of print.ABSTRACTExosomal proteins from the parental cells are considered to be promising biomarker sets for precise tumor diagnostics and monitoring. However, the accurate quantitative analysis of low-abundance exosomal proteins remains challenging due to the heterogeneity of clinical samples. Here, we standardized the exosomal concentration with a fluorogenic membrane probe and developed an aptamer-bivalent-cholesterol-mediated Proximity Entropy-driven Exosomal Protein Reporter (PEEPR). The proposed PEEPR enables the in-situ analysi...
Source: Biosensors and Bioelectronics - February 18, 2024 Category: Biotechnology Authors: Zhichao Fan Jie Zhou Qiuxia Shu Yan Dong Yingxue Li Tingrui Zhang Gang Bai Hua Yu Fanghao Lu Jianjun Li Xiang Zhao Source Type: research
Molecularly imprinted composite-based biosensor for the determination of SARS-CoV-2 nucleocapsid protein
This article aims to present a comparative study of three polypyrrole-based molecularly imprinted polymer (MIP) systems for the detection of the recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (rN). The rN is known for its relatively low propensity to mutate compared to other SARS-CoV-2 antigens. The aforementioned systems include screen-printed carbon electrodes (SPCE) modified with gold nanostructures (MIP1), platinum nanostructures (MIP2), and the unmodified SPCE (MIP3), which was used for control. Pulsed amperometric detection (PAD) was employed as the detection technique, ...
Source: Biosensors and Bioelectronics - February 18, 2024 Category: Biotechnology Authors: Maryia Drobysh Vilma Ratautaite Ernestas Brazys Almira Ramanaviciene Arunas Ramanavicius Source Type: research
Aptamer-bivalent-cholesterol-mediated proximity entropy-driven exosomal protein reporter for tumor diagnosis
Biosens Bioelectron. 2024 Feb 6;251:116104. doi: 10.1016/j.bios.2024.116104. Online ahead of print.ABSTRACTExosomal proteins from the parental cells are considered to be promising biomarker sets for precise tumor diagnostics and monitoring. However, the accurate quantitative analysis of low-abundance exosomal proteins remains challenging due to the heterogeneity of clinical samples. Here, we standardized the exosomal concentration with a fluorogenic membrane probe and developed an aptamer-bivalent-cholesterol-mediated Proximity Entropy-driven Exosomal Protein Reporter (PEEPR). The proposed PEEPR enables the in-situ analysi...
Source: Biosensors and Bioelectronics - February 18, 2024 Category: Biotechnology Authors: Zhichao Fan Jie Zhou Qiuxia Shu Yan Dong Yingxue Li Tingrui Zhang Gang Bai Hua Yu Fanghao Lu Jianjun Li Xiang Zhao Source Type: research
Passive trapping of biomolecules in hotspots with all-dielectric terahertz metamaterials
Biosens Bioelectron. 2024 Feb 12;251:116126. doi: 10.1016/j.bios.2024.116126. Online ahead of print.ABSTRACTElectromagnetic metamaterials feature the capability of squeezing photons into hotspot regions of high intensity near-field enhancement for strong light-matter interaction, underpinning the next generation of emerging biosensors. However, randomly dispersed biomolecules around the hotspots lead to weak interactions. Here, we demonstrate an all-silicon dielectric terahertz metamaterial sensor design capable of passively trapping biomoleculars into the resonant cavities confined with powerful electric field. Specifical...
Source: Biosensors and Bioelectronics - February 17, 2024 Category: Biotechnology Authors: Tingling Lin Yi Huang Shuncong Zhong Tingting Shi Fuwei Sun Yujie Zhong Qiuming Zeng Qiukun Zhang Daxiang Cui Source Type: research
A 3D-printed hollow microneedle-based electrochemical sensing device for in situ plant health monitoring
Biosens Bioelectron. 2024 Feb 13;251:116131. doi: 10.1016/j.bios.2024.116131. Online ahead of print.ABSTRACTPlant health monitoring is devised as a new concept to elucidate in situ physiological processes. The need for increased food production to nourish the growing global population is inconsistent with the dramatic impact of climate change, which hinders crop health and exacerbates plant stress. In this context, wearable sensors play a crucial role in assessing plant stress. Herein, we present a low-cost 3D-printed hollow microneedle array (HMA) patch as a sampling device coupled with biosensors based on screen-printing...
Source: Biosensors and Bioelectronics - February 17, 2024 Category: Biotechnology Authors: Marc Parrilla Amadeo Sena-Torralba Annemarijn Steijlen Sergi Morais Ángel Maquieira Karolien De Wael Source Type: research
Surface-functionalized SERS platform for deep learning-assisted diagnosis of Alzheimer's disease
Biosens Bioelectron. 2024 Feb 12;251:116128. doi: 10.1016/j.bios.2024.116128. Online ahead of print.ABSTRACTEarly diagnosis of Alzheimer's disease is crucial to stall the deterioration of brain function, but conventional diagnostic methods require complicated analytical procedures or inflict acute pain on the patient. Then, label-free Surface-enhanced Raman spectroscopy (SERS) analysis of blood-based biomarkers is a convenient alternative to rapidly obtain spectral information from biofluids. However, despite the rapid acquisition of spectral information from biofluids, it is challenging to distinguish spectral features of...
Source: Biosensors and Bioelectronics - February 17, 2024 Category: Biotechnology Authors: Minjoon Kim Sejoon Huh Hyung Joon Park Seunghee H Cho Min-Young Lee Sungho Jo Yeon Sik Jung Source Type: research
