Electrochemical biosensing platform based on AuNWs/rGO-CMC-PEDOT:PSS composite for the detection of superoxide anion released from living cells
This study provides an inovative design to obtain a biocompatible electrochemical sensing platform with plenty of immobilization sites for biomolecules, large surface area, high conductivity and flexibility.PMID:38522233 | DOI:10.1016/j.bios.2024.116228 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - March 24, 2024 Category: Biotechnology Authors: Renjun Jiang Jiaojiao Liu Xiaoqiang Liu Jadranka Travas Sejdic Source Type: research
Highly sensitive detection of the neurodegenerative biomarker Tau by using the concentration effect of the pyro-electrohydrodynamic jetting
Biosens Bioelectron. 2024 Mar 21;254:116234. doi: 10.1016/j.bios.2024.116234. Online ahead of print.ABSTRACTIt is largely documented that neurodegenerative diseases can be effectively treated only if early diagnosed. In this context, the structural changes of some biomolecules such as Tau, seem to play a key role in neurodegeneration mechanism becoming eligible targets for an early diagnosis. Post-translational modifications are responsible to drive the Tau protein towards a transition phase from a native disorder conformation into a preaggregation state, which then straight recruits the final fibrillization process. Here,...
Source: Biosensors and Bioelectronics - March 24, 2024 Category: Biotechnology Authors: Concetta Di Natale Sara Coppola Veronica Vespini Volodymyr Tkachenko Simone Russo Giuseppina Luciani Giuseppe Vitiello Francesca Ferranti Silvia Mari Pietro Ferraro Pier Luca Maffettone Simonetta Grilli Source Type: research
Establishment of a simple, sensitive, and specific ASFV detection method based on Pyrococcus furiosus argonaute
This study reports on a new nucleic acid assay method, termed REPD, which uses recombinase-aided amplification and restriction endonuclease-assisted Pyrococcus furiosus argonaute (PfAgo) detection. One-pot REPD was developed for the detection of ASFV. The one-pot REPD could detect a single copy of ASFV nucleic acid and showed no cross-reactivity with other pathogens. Detection in clinical samples was 100% consistent with the results of real-time PCR analysis. The results showed that the one-pot REPD assay is convenient, sensitive, specific, and potentially adaptable to the detection of ASFV. In summary, this study highligh...
Source: Biosensors and Bioelectronics - March 23, 2024 Category: Biotechnology Authors: Yu Zhao Ming Yang Changyu Zhou Boyan Guo Kailu Wang Cailiang Song Hongning Wang Source Type: research
Advancements in optical fiber-based wearable sensors for smart health monitoring
We present an overview of recent developments in optical fiber-based wearable sensors, focusing on two mechanisms: wavelength interrogation and intensity modulation for the detection of body temperature, pulse rate, respiration rate, body movements, and biomedical noninvasive fluids, with a thorough examination of their benefits and drawbacks. This review also focuses on improving working performance and application techniques for healthcare systems, including the integration of nanomaterials and the usage of the Internet of Things (IoT) with signal processing. Finally, the review concludes with a discussion of the future ...
Source: Biosensors and Bioelectronics - March 23, 2024 Category: Biotechnology Authors: Rajan Jha Pratik Mishra Santosh Kumar Source Type: research
Corrigendum to "Entrapment of uropathogenic E. coli cells into ultra-thin sol-gel matrices on gold thin films: A low cost alternative for impedimetric bacteria sensing" [Biosens Bioelectron. 124-125 (2019) 161-166 DOI: 10.1016/j.bios.2018.10.029]
Biosens Bioelectron. 2024 Mar 22:116226. doi: 10.1016/j.bios.2024.116226. Online ahead of print.NO ABSTRACTPMID:38521685 | DOI:10.1016/j.bios.2024.116226 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - March 23, 2024 Category: Biotechnology Authors: Hamed Jafari Mandana Amiri Esmat Abdi Saeid Latifi Navid Julie Bouckaert Roxana Jijie Rabah Boukherroub Sabine Szunerits Source Type: research
Establishment of a simple, sensitive, and specific ASFV detection method based on Pyrococcus furiosus argonaute
This study reports on a new nucleic acid assay method, termed REPD, which uses recombinase-aided amplification and restriction endonuclease-assisted Pyrococcus furiosus argonaute (PfAgo) detection. One-pot REPD was developed for the detection of ASFV. The one-pot REPD could detect a single copy of ASFV nucleic acid and showed no cross-reactivity with other pathogens. Detection in clinical samples was 100% consistent with the results of real-time PCR analysis. The results showed that the one-pot REPD assay is convenient, sensitive, specific, and potentially adaptable to the detection of ASFV. In summary, this study highligh...
Source: Biosensors and Bioelectronics - March 23, 2024 Category: Biotechnology Authors: Yu Zhao Ming Yang Changyu Zhou Boyan Guo Kailu Wang Cailiang Song Hongning Wang Source Type: research
Advancements in optical fiber-based wearable sensors for smart health monitoring
We present an overview of recent developments in optical fiber-based wearable sensors, focusing on two mechanisms: wavelength interrogation and intensity modulation for the detection of body temperature, pulse rate, respiration rate, body movements, and biomedical noninvasive fluids, with a thorough examination of their benefits and drawbacks. This review also focuses on improving working performance and application techniques for healthcare systems, including the integration of nanomaterials and the usage of the Internet of Things (IoT) with signal processing. Finally, the review concludes with a discussion of the future ...
Source: Biosensors and Bioelectronics - March 23, 2024 Category: Biotechnology Authors: Rajan Jha Pratik Mishra Santosh Kumar Source Type: research
Corrigendum to "Entrapment of uropathogenic E. coli cells into ultra-thin sol-gel matrices on gold thin films: A low cost alternative for impedimetric bacteria sensing" [Biosens Bioelectron. 124-125 (2019) 161-166 DOI: 10.1016/j.bios.2018.10.029]
Biosens Bioelectron. 2024 Mar 22:116226. doi: 10.1016/j.bios.2024.116226. Online ahead of print.NO ABSTRACTPMID:38521685 | DOI:10.1016/j.bios.2024.116226 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - March 23, 2024 Category: Biotechnology Authors: Hamed Jafari Mandana Amiri Esmat Abdi Saeid Latifi Navid Julie Bouckaert Roxana Jijie Rabah Boukherroub Sabine Szunerits Source Type: research
A handheld isothermal fluorescence detector for duplex visualization of aquatic pathogens via enhanced one-pot LAMP-PfAgo assay
Biosens Bioelectron. 2024 Mar 8;254:116187. doi: 10.1016/j.bios.2024.116187. Online ahead of print.ABSTRACTThe expansion of large-scale aquaculture has exacerbated the challenge of aquatic diseases, resulting in substantial economic losses annually. Currently, traditional laboratory-based diagnostic methods are time-consuming and costly, hindering on-site testing for individual farmers. We address this issue by developing a state-of-the-art handheld isothermal nucleic acid amplification device (WeD-1) capable of fluorescence tracking of reactions and integrating it with an enhanced one-pot Prokaryotic Argonaute based nucle...
Source: Biosensors and Bioelectronics - March 22, 2024 Category: Biotechnology Authors: Feibiao Pang Tao Zhang Fengyi Dai Kaizheng Wang Tianjiao Jiao Zuoying Zhang Liyi Zhang Mingli Liu Peng Hu Jinzhao Song Source Type: research
Urinary dengue NS1 detection on Au-decorated ZnO nanowire platform
Biosens Bioelectron. 2024 Mar 13;254:116218. doi: 10.1016/j.bios.2024.116218. Online ahead of print.ABSTRACTBiodetection for non-invasive diagnostics of fluids, especially urine, remains a challenge to scientists due to low target concentrations. And biological complexes of the detection target may contain contaminants that also interfere with any assay. Dengue non-structural 1 protein (Dengue NS1) is an important biomarker for dengue hemorrhagic fever and dengue shock syndrome. Here, we developed an Au-decorated nanowire platform and applied it with a sandwich fluorophore-linked immunosorbent well plate assay (FLISA) to d...
Source: Biosensors and Bioelectronics - March 22, 2024 Category: Biotechnology Authors: Kannika Sitthisuwannakul Ratchanon Sukthai Zetao Zhu Kazuki Nagashima Kunanon Chattrairat Supranee Phanthanawiboon Annop Klamchuen Sakon Rahong Yoshinobu Baba Takao Yasui Source Type: research
Synthesis of shape-programmable elastomer for a bioresorbable, wireless nerve stimulator
Biosens Bioelectron. 2024 Mar 16;254:116222. doi: 10.1016/j.bios.2024.116222. Online ahead of print.ABSTRACTMaterials that have the ability to manipulate shapes in response to stimuli such as heat, light, humidity and magnetism offer a means for versatile, sophisticated functions in soft robotics or biomedical implants, while such a reactive transformation has certain drawbacks including high operating temperatures, inherent rigidity and biological hazard. Herein, we introduce biodegradable, self-adhesive, shape-transformable poly (L-lactide-co-ε-caprolactone) (BSS-PLCL) that can be triggered via thermal stimulation near ...
Source: Biosensors and Bioelectronics - March 22, 2024 Category: Biotechnology Authors: Jun Hyeon Lim Won Bae Han Tae-Min Jang Gwan-Jin Ko Jeong-Woong Shin Sungkeun Han Heeseok Kang Chan-Hwi Eom So Jeong Choi Kaveti Rajaram Amay J Bandodkar Woon-Hong Yeo Suk-Won Hwang Source Type: research
Long-term stable pH sensor array with synergistic bilayer structure for 2D real-time mapping in cell culture monitoring
Biosens Bioelectron. 2024 Mar 18;254:116223. doi: 10.1016/j.bios.2024.116223. Online ahead of print.ABSTRACTPursuing accurate, swift, and durable pH sensors is important across numerous fields, encompassing healthcare, environmental surveillance, and agriculture. In particular, the emphasis on real-time pH monitoring during cell cultivation has become increasingly pronounced in the current scientific environment-a crucial element being diligently researched to ensure optimal cell production. Both polyaniline (PANi) and iridium oxide (IrOx) show their worth in pH sensing, yet they come with challenges. Single-PANi-layered p...
Source: Biosensors and Bioelectronics - March 22, 2024 Category: Biotechnology Authors: Jimin Lee Ira Soltis Sayre A Tillery Sung Hoon Lee Hodam Kim Woon-Hong Yeo Source Type: research
On-chip bioluminescence biosensor for the detection of microbial surface contamination
In this study, we introduce a biosensor based on a CMOS image sensor for ATP-mediated chemiluminescence detection. The original lens and IR filter were removed from the CMOS sensor revealing a 12 MP periodic microlens/pixel array on an area of 6.5 mm × 3.6 mm. UltraSnap swabs are used to collect samples from solid surfaces including personal electronic devices, and office and laboratory equipment. Samples mixed with chemiluminescence reagents were placed directly on the surface of the image sensor. Close proximity of the sample to the photodiode array leads to high photon collection efficiency. The population of microorga...
Source: Biosensors and Bioelectronics - March 22, 2024 Category: Biotechnology Authors: Reza Abbasi Meruyert Imanbekova Sebastian Wachsmann-Hogiu Source Type: research
Multifunctional fluorescence probe for simultaneous detection of viscosity, polarity, and ONOO < sup > - < /sup > and its bioimaging in vitro and vivo
Biosens Bioelectron. 2024 Mar 19;254:116233. doi: 10.1016/j.bios.2024.116233. Online ahead of print.ABSTRACTIntracellular microenvironment (viscosity and polarity) and peroxynitrite ions (ONOO-) are involved in maintaining cell morphology, cell function, and signaling so that it is crucial to explore their level changes in vitro and vivo. In this work, we designed and synthesized a mitochondria-targeted fluorescence probe XBL for monitoring the dynamic changes of viscosity, polarity, and ONOO- based on TICT and ICT mechanism. The fluorescence spectra showed obvious changes for polarity at 500 nm as well as ONOO- and viscos...
Source: Biosensors and Bioelectronics - March 22, 2024 Category: Biotechnology Authors: Yuan-Yuan Li Jia-Ling Hu Ji-Rou Wu Yi-Ru Wang Ai-Hong Zhang Yu-Wei Tan Ya-Jing Shang Ting Liang Min Li Ya-Li Meng Yan-Fei Kang Source Type: research
Ultrathin Parylene C-based sensitivity-gain nanoplasmonic sensor integrated on VCSEL for A β < sub > 42 < /sub > detection
This study presents a novel biosensor chip leveraging vertical cavity surface emitting laser (VCSEL) technology, with Parylene C serving as the antibody coupling layer and utilizing a streamlined one-step antibody modification method. Integration of Parylene C enhances chip sensitivity from 34.28 μW/RIU to 40.32 μW/RIU. Moreover, post-testing removal of Parylene C enables chip reusability without significant alteration of results. The sensor demonstrates effective detection of Aβ42, an Alzheimer's biomarker, exhibiting a linear range of 1-200 ng/mL and a detection limit of 0.26 ng/mL. These findings underscore the reusa...
Source: Biosensors and Bioelectronics - March 22, 2024 Category: Biotechnology Authors: Xiaoqing Lv Zhengtai Ma Wenhui Jiang Chengcheng Huang Jun Deng Huan Zhang Pengying Chang Yiyang Xie Source Type: research
