Two Birds with one Stone: Dual-mode immunoassay constructed using a novel emitter ethylene glycol-induced perylene diimide and a multifunctional ANS probe
In this study, ethylene glycol (EG) was used to induce the self-assembly of PDI supramolecules for the preparation of ultrathin EG-PDI nanosheets characterized by low crystallinity and weak stacking interaction. Notably, EG-PDI integrates luminescent and catalytic functions into one device, accelerating the interfacial electron transfer and the faster charge transfer kinetics of EG-PDI with K2S2O8. Furthermore, the narrow band gap of EG-PDI facilitates its excitation at an ultra-low potential (-0.3 V). To improve the efficiency of tumor marker analysis, multifunctional Au nanostars (ANS) was introduced both as an energy ac...
Source: Biosensors and Bioelectronics - February 25, 2024 Category: Biotechnology Authors: Wenjing Lai Shijie Yan Mingzhe Jiang Zhina Li Min Wang Chulei Zhao Chaoyun Ma Zhong Wei Chenglin Hong Source Type: research
Sensing of gene expression in live cells using electrical impedance spectroscopy and DNA-functionalized gold nanoparticles
Biosens Bioelectron. 2024 Jan 16;252:116041. doi: 10.1016/j.bios.2024.116041. Online ahead of print.ABSTRACTA novel electrical impedance spectroscopy-based method for non-destructive sensing of gene expression in living cells is presented. The approach used takes advantage of the robustness and responsiveness of electrical impedance spectroscopy and the highly specific and selective nature of DNA hybridization. The technique uses electrical impedance spectroscopy and gold nanoparticles functionalized with single-stranded DNA complementary to an mRNA of interest to provide reliable, real-time, and quantifiable data on gene ...
Source: Biosensors and Bioelectronics - February 24, 2024 Category: Biotechnology Authors: Kian Kadan-Jamal Aakash Jog Marios Sophocleous Tali Dotan Polina Frumin Tamar Kuperberg Goshen Silvia Schuster Adi Avni Yosi Shacham-Diamand Source Type: research
Capacitive immunosensing at gold nanoparticle-decorated reduced graphene oxide electrodes fabricated by one-step laser nanostructuration
This study introduces a capacitive biosensor that leverages laser-engraved reduced graphene oxide (rGO) electrodes decorated with gold nanoparticles (AuNPs). The fabrication involves laser-scribed GO-Au3+ films, yielding rGO-AuNP electrodes, seamlessly transferred onto a PET substrate via a press-stamping methodology. These electrodes have a remarkable affinity for biomolecular recognition after being functionalized with specific bioreceptors. For example, initial studies with human IgG antibodies confirm the detection capabilities of the biosensor using electrochemical capacitance spectroscopy. Furthermore, the biosensor ...
Source: Biosensors and Bioelectronics - February 24, 2024 Category: Biotechnology Authors: Danilo Echeverri Enric Calucho Jose Marrugo-Ram írez Rusl án Álvarez-Diduk Jahir Orozco Arben Merko çi Source Type: research
Fluorophore and nanozyme-functionalized DNA walking: A dual-mode DNA logic biocomputing platform for microRNA sensing in clinical samples
In this study, we introduce a fluorescent and photothermal dual-mode logic biosensing platform that integrates catalytic hairpin assembly (CHA), toehold-mediated stand displacement reaction (SDR) and a DNA walking machine. Dual identification and signal reporting modules are incorporated into DNA circuits, orchestrated by an AND Boolean logic gate operator and magnetic beads (MBs). In the presence of bispecific microRNAs (miRNAs), the AND logic gate activates, driving the DNA walking machine, and facilitating the collection of hairpin DNA stands modified with FAM fluorescent group and CeO2@Au nanoparticles. The CeO2@Au nan...
Source: Biosensors and Bioelectronics - February 24, 2024 Category: Biotechnology Authors: Jingyuan Yu Quanyi Liu Lijuan Qi Qi Fang Xudong Shang Xiaojun Zhang Yan Du Source Type: research
Development of the BioBattery: A novel enzyme fuel cell using a multicopper oxidase as an anodic enzyme
Biosens Bioelectron. 2024 Feb 8;252:116092. doi: 10.1016/j.bios.2024.116092. Online ahead of print.ABSTRACTThis work presents the development of an enzyme fuel cell, termed "BioBattery", that utilizes multicopper oxidases as the anodic enzyme in a non-diffusion limited system. We evaluated various enzyme variants as the anode, including multicopper oxidase from Pyrobaculum aerophilum, laccase from Trametes versicolor, and bilirubin oxidase from Myrothecium verrucaria. Several combinations of cathodes were also examined, focusing on the reduction of oxygen as the primary electron acceptor. The optimal pairing used multicopp...
Source: Biosensors and Bioelectronics - February 24, 2024 Category: Biotechnology Authors: Kartheek Batchu David Probst Takenori Satomura Koji Sode Source Type: research
Dual-responsive 3D DNA nanomachines cascaded hybridization chain reactions for novel self-powered flexible microRNA-detecting platform
Biosens Bioelectron. 2024 Feb 20;252:116149. doi: 10.1016/j.bios.2024.116149. Online ahead of print.ABSTRACTThe microRNA-21 is closely related to chromatin remodeling and epigenetic regulation. In this work, an efficient double-response 3D DNA nanomachine (DRDN) was assembled by co-immobilizing two different lengths of hairpin DNA on the surface of gold nanoparticles (AuNPs) to capture microRNA-21 (miRNA-21), recycle miRNA-21, and trigger hybridization chain reactions (HCR). This work reports the fabrication of a laser-scribed graphene (LSG) electrode with excellent flexibility and electrical conductivity by laser-scribing...
Source: Biosensors and Bioelectronics - February 23, 2024 Category: Biotechnology Authors: Futing Wang Chunxiao Zhang Suping Deng Yifei Jiang Penghui Zhang Hongfen Yang Li Xiang Yifan Lyu Ren Cai Weihong Tan Source Type: research
Amplification-free quantitative detection of genomic DNA using lateral flow strips for milk authentication
Biosens Bioelectron. 2024 Feb 18;252:116140. doi: 10.1016/j.bios.2024.116140. Online ahead of print.ABSTRACTWith the globalization and complexity of the food supply chain, the market is becoming increasingly competitive and food fraudulent activities are intensifying. The current state of food detection faced two primary challenges. Firstly, existing testing methods were predominantly laboratory-based, requiring complex procedures and precision instruments. Secondly, there was a lack of accurate and efficient quantitative detection methods. Taking cow's milk as an example, this study introduced a novel method for nucleic a...
Source: Biosensors and Bioelectronics - February 23, 2024 Category: Biotechnology Authors: Nan Wang Juan Zhang Bin Xiao Xiaoyun Sun Jiaci Chen Fengchun Huang Ailiang Chen Source Type: research
Toward nano-sized imprinted norepinephrine-derived biopolymer as artificial receptors for detecting IgG1 by surface plasmon resonance
Biosens Bioelectron. 2024 Feb 15;252:116133. doi: 10.1016/j.bios.2024.116133. Online ahead of print.ABSTRACTBio-based nanostructured molecularly imprinted polymers (nano-MIPs), also known as 'plastibodies', have a real potential to be used as alternatives to natural antibodies. These nanostructures have recently gained significant attention for diagnostic and therapeutic purposes. In this context, we have developed polynorepinephrine (PNE)-based nano-MIPs using an eco-friendly one-pot process for the sensitive and selective detection of a model biomolecule, immunoglobulin IgG1. We first investigated non-imprinted nanostruc...
Source: Biosensors and Bioelectronics - February 23, 2024 Category: Biotechnology Authors: Francesca Torrini Giovanni Ferraro Emiliano Fratini Pasquale Palladino Simona Scarano Maria Minunni Source Type: research
Novel high-dense microelectrode array based multimodal bioelectronic monitoring system for cardiac arrhythmia re-entry analysis
Biosens Bioelectron. 2024 Feb 14;252:116120. doi: 10.1016/j.bios.2024.116120. Online ahead of print.ABSTRACTIn recent decades, significant progress has been made in the treatment of heart diseases, particularly in the field of personalized medicine. Despite the development of genetic tests, phenotyping and risk stratification are performed based on clinical findings and invasive in vivo techniques, such as stimulation conduction mapping techniques and programmed ventricular pacing. Consequently, label-free non-invasive in vitro functional analysis systems are urgently needed for more accurate and effective in vitro risk st...
Source: Biosensors and Bioelectronics - February 23, 2024 Category: Biotechnology Authors: Sabine Schmidt Wener Li Mario Schubert Bj örn Binnewerg Christoph Pr önnecke Franziska D Zitzmann Martin Bulst Sebastian Wegner Matthias Meier Kaomei Guan Heinz-Georg Jahnke Source Type: research
Dual-responsive 3D DNA nanomachines cascaded hybridization chain reactions for novel self-powered flexible microRNA-detecting platform
Biosens Bioelectron. 2024 Feb 20;252:116149. doi: 10.1016/j.bios.2024.116149. Online ahead of print.ABSTRACTThe microRNA-21 is closely related to chromatin remodeling and epigenetic regulation. In this work, an efficient double-response 3D DNA nanomachine (DRDN) was assembled by co-immobilizing two different lengths of hairpin DNA on the surface of gold nanoparticles (AuNPs) to capture microRNA-21 (miRNA-21), recycle miRNA-21, and trigger hybridization chain reactions (HCR). This work reports the fabrication of a laser-scribed graphene (LSG) electrode with excellent flexibility and electrical conductivity by laser-scribing...
Source: Biosensors and Bioelectronics - February 23, 2024 Category: Biotechnology Authors: Futing Wang Chunxiao Zhang Suping Deng Yifei Jiang Penghui Zhang Hongfen Yang Li Xiang Yifan Lyu Ren Cai Weihong Tan Source Type: research
Amplification-free quantitative detection of genomic DNA using lateral flow strips for milk authentication
Biosens Bioelectron. 2024 Feb 18;252:116140. doi: 10.1016/j.bios.2024.116140. Online ahead of print.ABSTRACTWith the globalization and complexity of the food supply chain, the market is becoming increasingly competitive and food fraudulent activities are intensifying. The current state of food detection faced two primary challenges. Firstly, existing testing methods were predominantly laboratory-based, requiring complex procedures and precision instruments. Secondly, there was a lack of accurate and efficient quantitative detection methods. Taking cow's milk as an example, this study introduced a novel method for nucleic a...
Source: Biosensors and Bioelectronics - February 23, 2024 Category: Biotechnology Authors: Nan Wang Juan Zhang Bin Xiao Xiaoyun Sun Jiaci Chen Fengchun Huang Ailiang Chen Source Type: research
Toward nano-sized imprinted norepinephrine-derived biopolymer as artificial receptors for detecting IgG1 by surface plasmon resonance
Biosens Bioelectron. 2024 Feb 15;252:116133. doi: 10.1016/j.bios.2024.116133. Online ahead of print.ABSTRACTBio-based nanostructured molecularly imprinted polymers (nano-MIPs), also known as 'plastibodies', have a real potential to be used as alternatives to natural antibodies. These nanostructures have recently gained significant attention for diagnostic and therapeutic purposes. In this context, we have developed polynorepinephrine (PNE)-based nano-MIPs using an eco-friendly one-pot process for the sensitive and selective detection of a model biomolecule, immunoglobulin IgG1. We first investigated non-imprinted nanostruc...
Source: Biosensors and Bioelectronics - February 23, 2024 Category: Biotechnology Authors: Francesca Torrini Giovanni Ferraro Emiliano Fratini Pasquale Palladino Simona Scarano Maria Minunni Source Type: research
Novel high-dense microelectrode array based multimodal bioelectronic monitoring system for cardiac arrhythmia re-entry analysis
Biosens Bioelectron. 2024 Feb 14;252:116120. doi: 10.1016/j.bios.2024.116120. Online ahead of print.ABSTRACTIn recent decades, significant progress has been made in the treatment of heart diseases, particularly in the field of personalized medicine. Despite the development of genetic tests, phenotyping and risk stratification are performed based on clinical findings and invasive in vivo techniques, such as stimulation conduction mapping techniques and programmed ventricular pacing. Consequently, label-free non-invasive in vitro functional analysis systems are urgently needed for more accurate and effective in vitro risk st...
Source: Biosensors and Bioelectronics - February 23, 2024 Category: Biotechnology Authors: Sabine Schmidt Wener Li Mario Schubert Bj örn Binnewerg Christoph Pr önnecke Franziska D Zitzmann Martin Bulst Sebastian Wegner Matthias Meier Kaomei Guan Heinz-Georg Jahnke Source Type: research
A versatile upconversion-based multimode lateral flow platform for rapid and ultrasensitive detection of microRNA towards health monitoring
Biosens Bioelectron. 2024 Feb 16;252:116135. doi: 10.1016/j.bios.2024.116135. Online ahead of print.ABSTRACTMicroRNAs are small single-stranded RNA molecules associated with gene expression and immune response, suggesting their potential as biomarkers for health monitoring. Herein, we designed a novel upconversion-based multimode lateral flow assay (LFA) system to detect microRNAs in body fluids by simultaneously producing three unique signals within a detection strip. The core-shell Au-DTNB@Ag nanoparticles act as both the Raman reporters and acceptors, quenching fluorescence from upconversion nanoparticles (UCNPs, NaYF4:...
Source: Biosensors and Bioelectronics - February 22, 2024 Category: Biotechnology Authors: Cong Chen Songtao Hu Lulu Tian Manlin Qi Zhiyong Chang Liang Li Lin Wang Biao Dong Source Type: research
Wireless electrochemiluminescent biosensors: Powering innovation with smartphone technology
Biosens Bioelectron. 2024 Feb 1;252:116083. doi: 10.1016/j.bios.2024.116083. Online ahead of print.ABSTRACTEnergy supply and sensor response acquisition can be performed wirelessly, enabling biosensors as Internet of Thing (IoT) tools by linking wireless power supply and electrochemical sensors. Here, we used the electromagnetic induction method to clarify the conditions under which electrochemiluminescence is induced by a simple potential modulation circuit without an integrated circuit on the electrode chip that receives the power. Initially, the potential waveform obtained in a circuit with inductance and capacitance co...
Source: Biosensors and Bioelectronics - February 22, 2024 Category: Biotechnology Authors: Eiichi Tamiya Shuto Osaki Hidenori Nagai Source Type: research
