Fiber-optic immunosensor based on a Fabry-Perot interferometer for single-molecule detection of biomarkers
Biosens Bioelectron. 2024 Apr 1;255:116265. doi: 10.1016/j.bios.2024.116265. Online ahead of print.ABSTRACTImmunosensors capable of ultralow-concentration and single-molecule detection of biomarkers are garnering attention for the early diagnosis of cancer. Herein, a fiber-optic Fabry-Perot interferometer (FPI)-based immunosensor was used for the first time for single-molecule detection of progastrin-releasing peptide (ProGRP). The cascaded FPI structure of the immunosensor introduces a high-order harmonic Vernier effect (HVE). A piece of a side-polished D-shaped hollow-core photonic crystal fiber (HCPCF) was used as a sen...
Source: Biosensors and Bioelectronics - April 3, 2024 Category: Biotechnology Authors: Haiming Qiu Yong Yao Yongkang Dong Jiajun Tian Source Type: research
Light-driven, bias-free nitrogenase-based bioelectrochemical cell for ammonia generation
Biosens Bioelectron. 2024 Mar 27;255:116254. doi: 10.1016/j.bios.2024.116254. Online ahead of print.ABSTRACTNitrogen fixation is a key process that sustains life on Earth. Nitrogenase is the sole enzyme capable of fixing nitrogen under ambient conditions. Extensive research efforts have been dedicated to elucidating the enzyme mechanism and its artificial activation through high applied voltage, photochemistry, or strong reducing agents. Harnessing light irradiation to minimize the required external bias can lower the process's high energy investment. Herein, we present the development of photo-bioelectrochemical cells (PB...
Source: Biosensors and Bioelectronics - April 3, 2024 Category: Biotechnology Authors: Matan M Meirovich Oren Bachar Mor Shemesh Yifat Cohen Alice Popik Omer Yehezkeli Source Type: research
Advances in heart failure monitoring: Biosensors targeting molecular markers in peripheral bio-fluids
This article provides an up-to-date overview of the biomarkers associated with the six main heart failure etiology pathways. Plasma natriuretic peptides (BNP and NT-proBNP) and cardiac troponins (cTnT, cTnl) are still analyzed as gold-standard markers for heart failure. Other complementary biomarkers include growth differentiation factor 15 (GDF-15), circulating Galactose Lectin 3 (Gal-3), soluble interleukin (sST2), C-reactive protein (CRP), and tumor necrosis factor-alpha (TNF-α). For these biomarkers, the electrochemical biosensors have exhibited sufficient sensitivity, detection limit, and specificity. This review sys...
Source: Biosensors and Bioelectronics - April 3, 2024 Category: Biotechnology Authors: Tao Dong Wangang Zhu Zhaochu Yang Nuno Miguel Matos Pires Qijing Lin Weixuan Jing Libo Zhao Xueyong Wei Zhuangde Jiang Source Type: research
Fiber-optic immunosensor based on a Fabry-Perot interferometer for single-molecule detection of biomarkers
Biosens Bioelectron. 2024 Apr 1;255:116265. doi: 10.1016/j.bios.2024.116265. Online ahead of print.ABSTRACTImmunosensors capable of ultralow-concentration and single-molecule detection of biomarkers are garnering attention for the early diagnosis of cancer. Herein, a fiber-optic Fabry-Perot interferometer (FPI)-based immunosensor was used for the first time for single-molecule detection of progastrin-releasing peptide (ProGRP). The cascaded FPI structure of the immunosensor introduces a high-order harmonic Vernier effect (HVE). A piece of a side-polished D-shaped hollow-core photonic crystal fiber (HCPCF) was used as a sen...
Source: Biosensors and Bioelectronics - April 3, 2024 Category: Biotechnology Authors: Haiming Qiu Yong Yao Yongkang Dong Jiajun Tian Source Type: research
Light-driven, bias-free nitrogenase-based bioelectrochemical cell for ammonia generation
Biosens Bioelectron. 2024 Mar 27;255:116254. doi: 10.1016/j.bios.2024.116254. Online ahead of print.ABSTRACTNitrogen fixation is a key process that sustains life on Earth. Nitrogenase is the sole enzyme capable of fixing nitrogen under ambient conditions. Extensive research efforts have been dedicated to elucidating the enzyme mechanism and its artificial activation through high applied voltage, photochemistry, or strong reducing agents. Harnessing light irradiation to minimize the required external bias can lower the process's high energy investment. Herein, we present the development of photo-bioelectrochemical cells (PB...
Source: Biosensors and Bioelectronics - April 3, 2024 Category: Biotechnology Authors: Matan M Meirovich Oren Bachar Mor Shemesh Yifat Cohen Alice Popik Omer Yehezkeli Source Type: research
Dual toeholds regulated CRISPR-Cas12a sensing platform for ApoE single nucleotide polymorphisms genotyping
Biosens Bioelectron. 2024 Mar 28;255:116255. doi: 10.1016/j.bios.2024.116255. Online ahead of print.ABSTRACTSingle nucleotide polymorphisms (SNPs) are closely associated with many biological processes, including genetic disease, tumorigenesis, and drug metabolism. Accurate and efficient SNP determination has been proved pivotal in pharmacogenomics and diagnostics. Herein, a universal and high-fidelity genotyping platform is established based on the dual toeholds regulated Cas12a sensing methodology. Different from the conventional single stranded or double stranded activation mode, the dual toeholds regulated mode overcome...
Source: Biosensors and Bioelectronics - April 2, 2024 Category: Biotechnology Authors: Yuedong Zhu Yanan Lin Bin Gong Yan Zhang Gaoxing Su Yanyan Yu Source Type: research
Advancing abiotic stress monitoring in plants with a wearable non-destructive real-time salicylic acid laser-induced-graphene sensor
Biosens Bioelectron. 2024 Mar 31;255:116261. doi: 10.1016/j.bios.2024.116261. Online ahead of print.ABSTRACTDrought and salinity stresses present significant challenges that exert a severe impact on crop productivity worldwide. Understanding the dynamics of salicylic acid (SA), a vital phytohormone involved in stress response, can provide valuable insights into the mechanisms of plant adaptation to cope with these challenging conditions. This paper describes and tests a sensor system that enables real-time and non-invasive monitoring of SA content in avocado plants exposed to drought and salinity. By using a reverse iontop...
Source: Biosensors and Bioelectronics - April 2, 2024 Category: Biotechnology Authors: Sammy A Perdomo Drochss Pettry Valencia Gabriel Esteban Velez Andres Jaramillo-Botero Source Type: research
Dual toeholds regulated CRISPR-Cas12a sensing platform for ApoE single nucleotide polymorphisms genotyping
Biosens Bioelectron. 2024 Mar 28;255:116255. doi: 10.1016/j.bios.2024.116255. Online ahead of print.ABSTRACTSingle nucleotide polymorphisms (SNPs) are closely associated with many biological processes, including genetic disease, tumorigenesis, and drug metabolism. Accurate and efficient SNP determination has been proved pivotal in pharmacogenomics and diagnostics. Herein, a universal and high-fidelity genotyping platform is established based on the dual toeholds regulated Cas12a sensing methodology. Different from the conventional single stranded or double stranded activation mode, the dual toeholds regulated mode overcome...
Source: Biosensors and Bioelectronics - April 2, 2024 Category: Biotechnology Authors: Yuedong Zhu Yanan Lin Bin Gong Yan Zhang Gaoxing Su Yanyan Yu Source Type: research
Advancing abiotic stress monitoring in plants with a wearable non-destructive real-time salicylic acid laser-induced-graphene sensor
Biosens Bioelectron. 2024 Mar 31;255:116261. doi: 10.1016/j.bios.2024.116261. Online ahead of print.ABSTRACTDrought and salinity stresses present significant challenges that exert a severe impact on crop productivity worldwide. Understanding the dynamics of salicylic acid (SA), a vital phytohormone involved in stress response, can provide valuable insights into the mechanisms of plant adaptation to cope with these challenging conditions. This paper describes and tests a sensor system that enables real-time and non-invasive monitoring of SA content in avocado plants exposed to drought and salinity. By using a reverse iontop...
Source: Biosensors and Bioelectronics - April 2, 2024 Category: Biotechnology Authors: Sammy A Perdomo Drochss Pettry Valencia Gabriel Esteban Velez Andres Jaramillo-Botero Source Type: research
A spatial-potential resolved bipolar electrode electrochemiluminescence biosensor based on polarity conversion for dual-mode detection of miRNA-122 and CEA
Biosens Bioelectron. 2024 Mar 28;255:116258. doi: 10.1016/j.bios.2024.116258. Online ahead of print.ABSTRACTIn this work, a spatial-potential resolved bipolar electrode electrochemiluminescence (BPE-ECL) biosensor based on polarity conversion strategy and CuHCF electrocatalyst was constructed for dual-mode detection of miRNA-122 and carcinoembryonic antigen (CEA). ECL technology was firstly used to systematically study the polarity conversion of BPE. It was found that changing the polarity of the driving voltage would cause the polarity change of BPE, and led to the change of the luminescent position of Ru(bpy)32+. As a "p...
Source: Biosensors and Bioelectronics - March 31, 2024 Category: Biotechnology Authors: Hongkun Li Qianqian Cai Zhikang Li Guifen Jie Hong Zhou Source Type: research
Proximity labeling-assisted click conjugation for electrochemical analysis of specific subpopulations in circulating extracellular vesicles
Biosens Bioelectron. 2024 Mar 28;255:116245. doi: 10.1016/j.bios.2024.116245. Online ahead of print.ABSTRACTSensitive and accurate analysis of specific subpopulations in circulating extracellular vesicles (EVs) can provide a wealth of information for cancer diagnosis and management. Thus, we propose herein a new electrochemical biosensing method based on a proximity labeling-assisted click conjugation strategy. The method's core design is use of antibody-guided proximity labeling to equip target EVs with a large amount of alkyne groups, so that azide-tagged silver nanoparticles can be accurately loaded onto target EV surfa...
Source: Biosensors and Bioelectronics - March 31, 2024 Category: Biotechnology Authors: Yue Cao Liang Zhou Guozhang Zhou Wensheng Liu Haiyan Cui Ya Cao Xiaolei Zuo Jing Zhao Source Type: research
Nonwoven fiber meshes for oxygen sensing
Biosens Bioelectron. 2024 Mar 27;255:116198. doi: 10.1016/j.bios.2024.116198. Online ahead of print.ABSTRACTAccurate oxygen sensing and cost-effective fabrication are crucial for the adoption of wearable devices inside and outside the clinical setting. Here we introduce a simple strategy to create nonwoven polymeric fibrous mats for a notable contribution towards addressing this need. Although morphological manipulation of polymers for cell culture proliferation is commonplace, especially in the field of regenerative medicine, non-woven structures have not been used for oxygen sensing. We used an airbrush spraying, i.e. so...
Source: Biosensors and Bioelectronics - March 31, 2024 Category: Biotechnology Authors: Nikolaos Salaris Wenqing Chen Paul Haigh Lorenzo Caciolli Giovanni Giuseppe Giobbe Paolo De Coppi Ioannis Papakonstantinou Manish K Tiwari Source Type: research
Layer-by-layer growth of Cu < sub > 3 < /sub > (HHTP) < sub > 2 < /sub > films on Cu(OH) < sub > 2 < /sub > nanowire arrays for high performance ascorbic acid sensing
Biosens Bioelectron. 2024 Mar 28;255:116256. doi: 10.1016/j.bios.2024.116256. Online ahead of print.ABSTRACTGrowing three-dimensional (3D) metal organic frameworks (MOFs) via heterogeneous epitaxial growth on metal hydroxide arrays are effective for constructing electrochemical sensor. However, the growth of MOFs is difficult to control, resulting in thick and irregular morphologies and even damage the metal hydroxide template. In this work, Cu3(HHTP)2 (HHTP = 2, 3, 6, 7, 10, 11-hexahydroxytriphenylene) films with controllable thickness and morphology were successfully prepared on Cu(OH)2 nanowire arrays (NWAs) through lay...
Source: Biosensors and Bioelectronics - March 31, 2024 Category: Biotechnology Authors: Chenhuinan Wei Zhuo Wang Yurun Hu Jingqi Huang Yang Zhang Huihu Wang Qiming Liu Ziyang Yu Source Type: research
A spatial-potential resolved bipolar electrode electrochemiluminescence biosensor based on polarity conversion for dual-mode detection of miRNA-122 and CEA
Biosens Bioelectron. 2024 Mar 28;255:116258. doi: 10.1016/j.bios.2024.116258. Online ahead of print.ABSTRACTIn this work, a spatial-potential resolved bipolar electrode electrochemiluminescence (BPE-ECL) biosensor based on polarity conversion strategy and CuHCF electrocatalyst was constructed for dual-mode detection of miRNA-122 and carcinoembryonic antigen (CEA). ECL technology was firstly used to systematically study the polarity conversion of BPE. It was found that changing the polarity of the driving voltage would cause the polarity change of BPE, and led to the change of the luminescent position of Ru(bpy)32+. As a "p...
Source: Biosensors and Bioelectronics - March 31, 2024 Category: Biotechnology Authors: Hongkun Li Qianqian Cai Zhikang Li Guifen Jie Hong Zhou Source Type: research
Proximity labeling-assisted click conjugation for electrochemical analysis of specific subpopulations in circulating extracellular vesicles
Biosens Bioelectron. 2024 Mar 28;255:116245. doi: 10.1016/j.bios.2024.116245. Online ahead of print.ABSTRACTSensitive and accurate analysis of specific subpopulations in circulating extracellular vesicles (EVs) can provide a wealth of information for cancer diagnosis and management. Thus, we propose herein a new electrochemical biosensing method based on a proximity labeling-assisted click conjugation strategy. The method's core design is use of antibody-guided proximity labeling to equip target EVs with a large amount of alkyne groups, so that azide-tagged silver nanoparticles can be accurately loaded onto target EV surfa...
Source: Biosensors and Bioelectronics - March 31, 2024 Category: Biotechnology Authors: Yue Cao Liang Zhou Guozhang Zhou Wensheng Liu Haiyan Cui Ya Cao Xiaolei Zuo Jing Zhao Source Type: research
