Insulin biotrapping using plasmofluidic optical fiber chips: A benchmark
This study lays the foundation for the detection of punctual molecular interactions and opens the way towards the detection of spatially and temporally localized events on the surface of optical probes.PMID:38507927 | DOI:10.1016/j.bios.2024.116189 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - March 20, 2024 Category: Biotechnology Authors: M édéric Loyez Hadrien Fasseaux Maxime Lobry Ruddy Wattiez Christophe Caucheteur Source Type: research
A generalizable sensing platform based on molecularly imprinted polymer-aptamer double recognition and nanoenzyme assisted photoelectrochemical-colorimetric dual-mode detection
In this study, a generalizable photoelectrochemical-colorimetric dual-mode sensing platform was constructed based on the synergistic effects of a molecularly imprinted polymer (MIP)-aptamer sandwich structure and nanoenzymes. The MIP functionalized peroxidase-like Fe3O4 (Fe3O4@MIPs) and alkaline phosphatase mimic Zr-MOF labeled aptamer (Zr-mof@Apt) were used as the recognition elements. By selectively accumulating dibutyl phthalate (DBP), a small molecule target model, on Fe3O4@MIPs, the formation of Zr-MOF@Apt-DBP- Fe3O4@MIPs sandwich structure was triggered. Fe3O4@MIPs oxidized TMB to form blue-colored oxTMB. However, up...
Source: Biosensors and Bioelectronics - March 20, 2024 Category: Biotechnology Authors: Ying-Zhuo Shen Wen Zheng Xie Zheng Wang Kang Ping Ning Zheng Ping Ji Hong Bo Li Xiao-Ya Hu Cheng Ma Xu Qin Source Type: research
Biosensors; a novel concept in real-time detection of autophagy
Biosens Bioelectron. 2024 Mar 16;254:116204. doi: 10.1016/j.bios.2024.116204. Online ahead of print.ABSTRACTAutophagy is an early-stage response with self-degradation properties against several insulting conditions. To date, the critical role of autophagy has been well-documented in physiological and pathological conditions. This process involves various signaling and functional biomolecules, which are involved in different steps of the autophagic response. During recent decades, a range of biochemical analyses, chemical assays, and varied imaging techniques have been used for monitoring this pathway. Due to the complexity...
Source: Biosensors and Bioelectronics - March 20, 2024 Category: Biotechnology Authors: Hassan Nasrollahpour Arezoo Mirzaie Maryam Sharifi Aysa Rezabakhsh Balal Khalilzadeh Reza Rahbarghazi Hadi Yousefi Daniel J Klionsky Source Type: research
Insulin biotrapping using plasmofluidic optical fiber chips: A benchmark
This study lays the foundation for the detection of punctual molecular interactions and opens the way towards the detection of spatially and temporally localized events on the surface of optical probes.PMID:38507927 | DOI:10.1016/j.bios.2024.116189 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - March 20, 2024 Category: Biotechnology Authors: M édéric Loyez Hadrien Fasseaux Maxime Lobry Ruddy Wattiez Christophe Caucheteur Source Type: research
A generalizable sensing platform based on molecularly imprinted polymer-aptamer double recognition and nanoenzyme assisted photoelectrochemical-colorimetric dual-mode detection
In this study, a generalizable photoelectrochemical-colorimetric dual-mode sensing platform was constructed based on the synergistic effects of a molecularly imprinted polymer (MIP)-aptamer sandwich structure and nanoenzymes. The MIP functionalized peroxidase-like Fe3O4 (Fe3O4@MIPs) and alkaline phosphatase mimic Zr-MOF labeled aptamer (Zr-mof@Apt) were used as the recognition elements. By selectively accumulating dibutyl phthalate (DBP), a small molecule target model, on Fe3O4@MIPs, the formation of Zr-MOF@Apt-DBP- Fe3O4@MIPs sandwich structure was triggered. Fe3O4@MIPs oxidized TMB to form blue-colored oxTMB. However, up...
Source: Biosensors and Bioelectronics - March 20, 2024 Category: Biotechnology Authors: Ying-Zhuo Shen Wen Zheng Xie Zheng Wang Kang Ping Ning Zheng Ping Ji Hong Bo Li Xiao-Ya Hu Cheng Ma Xu Qin Source Type: research
Biosensors; a novel concept in real-time detection of autophagy
Biosens Bioelectron. 2024 Mar 16;254:116204. doi: 10.1016/j.bios.2024.116204. Online ahead of print.ABSTRACTAutophagy is an early-stage response with self-degradation properties against several insulting conditions. To date, the critical role of autophagy has been well-documented in physiological and pathological conditions. This process involves various signaling and functional biomolecules, which are involved in different steps of the autophagic response. During recent decades, a range of biochemical analyses, chemical assays, and varied imaging techniques have been used for monitoring this pathway. Due to the complexity...
Source: Biosensors and Bioelectronics - March 20, 2024 Category: Biotechnology Authors: Hassan Nasrollahpour Arezoo Mirzaie Maryam Sharifi Aysa Rezabakhsh Balal Khalilzadeh Reza Rahbarghazi Hadi Yousefi Daniel J Klionsky Source Type: research
Renin imprinted Poly(methyldopa) for biomarker detection and disease therapy
Biosens Bioelectron. 2024 Mar 14;254:116225. doi: 10.1016/j.bios.2024.116225. Online ahead of print.ABSTRACTConventional molecularly imprinted polymers (MIPs) perform their functions principally depended on their three dimensional (3D) imprinted cavities (recognition sites) of templates. Here, retaining the function of recognition sites resulted from the imprinting of template molecules, the role of functional monomers is explored and expanded. Briefly, a class of dual-functional renin imprinted poly(methyldopa) (RMIP) is prepared, consisting of a drug-type function monomer (methyldopa, clinical high blood pressure drug) a...
Source: Biosensors and Bioelectronics - March 19, 2024 Category: Biotechnology Authors: Xiaofeng Sun Tianqing Hu Yuexia Bai Tianyu Cao Shuai Wang Wei Hu Huan Yang Xiliang Luo Min Cui Source Type: research
Renin imprinted Poly(methyldopa) for biomarker detection and disease therapy
Biosens Bioelectron. 2024 Mar 14;254:116225. doi: 10.1016/j.bios.2024.116225. Online ahead of print.ABSTRACTConventional molecularly imprinted polymers (MIPs) perform their functions principally depended on their three dimensional (3D) imprinted cavities (recognition sites) of templates. Here, retaining the function of recognition sites resulted from the imprinting of template molecules, the role of functional monomers is explored and expanded. Briefly, a class of dual-functional renin imprinted poly(methyldopa) (RMIP) is prepared, consisting of a drug-type function monomer (methyldopa, clinical high blood pressure drug) a...
Source: Biosensors and Bioelectronics - March 19, 2024 Category: Biotechnology Authors: Xiaofeng Sun Tianqing Hu Yuexia Bai Tianyu Cao Shuai Wang Wei Hu Huan Yang Xiliang Luo Min Cui Source Type: research
Development of closed bipolar electrode based L-lactate sensor employing quasi-direct electron transfer type enzyme with cyclic voltammetry
Biosens Bioelectron. 2024 Mar 7;254:116197. doi: 10.1016/j.bios.2024.116197. Online ahead of print.ABSTRACTHerein, we present a proof-of-concept of an enzyme sensor combining closed bipolar electrode system with quasi-direct electron transfer (DET) type enzyme. The closed bipolar electrode system was tested using cyclic voltammetry, with L-lactate as a model substrate. L-Lactate was detected through measurement of the change in junction potential across the bipolar electrode. This change in junction potential was caused by reduction of amino reactive phenazine ethosulfate conjugated to Aerococcus vilidans derived engineere...
Source: Biosensors and Bioelectronics - March 17, 2024 Category: Biotechnology Authors: David Probst Koji Sode Source Type: research
Development of closed bipolar electrode based L-lactate sensor employing quasi-direct electron transfer type enzyme with cyclic voltammetry
Biosens Bioelectron. 2024 Mar 7;254:116197. doi: 10.1016/j.bios.2024.116197. Online ahead of print.ABSTRACTHerein, we present a proof-of-concept of an enzyme sensor combining closed bipolar electrode system with quasi-direct electron transfer (DET) type enzyme. The closed bipolar electrode system was tested using cyclic voltammetry, with L-lactate as a model substrate. L-Lactate was detected through measurement of the change in junction potential across the bipolar electrode. This change in junction potential was caused by reduction of amino reactive phenazine ethosulfate conjugated to Aerococcus vilidans derived engineere...
Source: Biosensors and Bioelectronics - March 17, 2024 Category: Biotechnology Authors: David Probst Koji Sode Source Type: research
Aggregation-induced emission of TTCPy-3: A novel approach for eradicating Nocardia seriolae infections in aquatic fishes
This study introduces luminogens with aggregation-induced emission (AIE) as an innovative and non-antibiotic therapy for N. seriolae. Specifically, the AIE photosensitizer, TTCPy-3 is employed against N. seriolae. We evaluated the antibacterial activity of TTCPy-3 and investigated the killing mechanism against N. seriolae, emphasizing its ability to aggregate within the bacterium and produce reactive oxygen species (ROS). TTCPy-3 could effectively aggregate in N. seriolae, generate ROS, and perform real-time imaging of the bacteria. A bactericidal efficiency of 100% was observed while concentrations exceeding 4 μM in the ...
Source: Biosensors and Bioelectronics - March 16, 2024 Category: Biotechnology Authors: Zhigang Liu Junling Zhang Xiaona Ma Miao Wang Lijin Jiang Meiyan Zhang Maixin Lu Ouqin Chang Jianmeng Cao Xiaoli Ke Mengmeng Yi Source Type: research
Label-free plasmonic spectral profiling of serum DNA
Biosens Bioelectron. 2024 Mar 13;254:116199. doi: 10.1016/j.bios.2024.116199. Online ahead of print.ABSTRACTGenetic and epigenetic modifications are linked to the activation of oncogenes and inactivation of tumor suppressor genes. Likewise, the associated molecular alternations can best inform precision medicine for personalized tumor treatment. Therefore, performing characterization of genetic and epigenetic alternations at the molecular level represents a crucial step in early diagnosis and/or therapeutics of cancer. However, the prevailing methods for DNA analysis involve a series of tedious and complicated steps, in wh...
Source: Biosensors and Bioelectronics - March 16, 2024 Category: Biotechnology Authors: Peng Zheng Piyush Raj Le Liang Lintong Wu Santosh Kumar Paidi Jeong Hee Kim Ishan Barman Source Type: research
Aggregation-induced emission of TTCPy-3: A novel approach for eradicating Nocardia seriolae infections in aquatic fishes
This study introduces luminogens with aggregation-induced emission (AIE) as an innovative and non-antibiotic therapy for N. seriolae. Specifically, the AIE photosensitizer, TTCPy-3 is employed against N. seriolae. We evaluated the antibacterial activity of TTCPy-3 and investigated the killing mechanism against N. seriolae, emphasizing its ability to aggregate within the bacterium and produce reactive oxygen species (ROS). TTCPy-3 could effectively aggregate in N. seriolae, generate ROS, and perform real-time imaging of the bacteria. A bactericidal efficiency of 100% was observed while concentrations exceeding 4 μM in the ...
Source: Biosensors and Bioelectronics - March 16, 2024 Category: Biotechnology Authors: Zhigang Liu Junling Zhang Xiaona Ma Miao Wang Lijin Jiang Meiyan Zhang Maixin Lu Ouqin Chang Jianmeng Cao Xiaoli Ke Mengmeng Yi Source Type: research
Label-free plasmonic spectral profiling of serum DNA
Biosens Bioelectron. 2024 Mar 13;254:116199. doi: 10.1016/j.bios.2024.116199. Online ahead of print.ABSTRACTGenetic and epigenetic modifications are linked to the activation of oncogenes and inactivation of tumor suppressor genes. Likewise, the associated molecular alternations can best inform precision medicine for personalized tumor treatment. Therefore, performing characterization of genetic and epigenetic alternations at the molecular level represents a crucial step in early diagnosis and/or therapeutics of cancer. However, the prevailing methods for DNA analysis involve a series of tedious and complicated steps, in wh...
Source: Biosensors and Bioelectronics - March 16, 2024 Category: Biotechnology Authors: Peng Zheng Piyush Raj Le Liang Lintong Wu Santosh Kumar Paidi Jeong Hee Kim Ishan Barman Source Type: research
Integrating target-responsive microfluidic-based biosensing chip with smartphone for simultaneous quantification of multiple fluoroquinolones
In this study, the background-free properties of upconversion nanoparticles (UCNPs), the high specificity of the target aptamer (Apt), and the high quenching properties of graphene oxide (GO) were integrated into a microfluidic-based fluorescence biosensing chip (MFBC). Interestingly, the microfluidic channels of the MFBC were prepared by laser-printing technology without the need for complex preparation processes and additional specialized equipment. The target-responsive fluorescence biosensing probes loaded on the MFBC were prepared by self-assembly of the UCNPs-Apt complex with GO based on π-π stacking interactions, ...
Source: Biosensors and Bioelectronics - March 15, 2024 Category: Biotechnology Authors: Mingming Zhang Xue Wang Shuangshuang Liu Tahreem Riaz Quansheng Chen Qin Ouyang Source Type: research
