Urinary dengue NS1 detection on Au-decorated ZnO nanowire platform
Biosens Bioelectron. 2024 Mar 13;254:116218. doi: 10.1016/j.bios.2024.116218. Online ahead of print.ABSTRACTBiodetection for non-invasive diagnostics of fluids, especially urine, remains a challenge to scientists due to low target concentrations. And biological complexes of the detection target may contain contaminants that also interfere with any assay. Dengue non-structural 1 protein (Dengue NS1) is an important biomarker for dengue hemorrhagic fever and dengue shock syndrome. Here, we developed an Au-decorated nanowire platform and applied it with a sandwich fluorophore-linked immunosorbent well plate assay (FLISA) to d...
Source: Biosensors and Bioelectronics - March 22, 2024 Category: Biotechnology Authors: Kannika Sitthisuwannakul Ratchanon Sukthai Zetao Zhu Kazuki Nagashima Kunanon Chattrairat Supranee Phanthanawiboon Annop Klamchuen Sakon Rahong Yoshinobu Baba Takao Yasui Source Type: research
Synthesis of shape-programmable elastomer for a bioresorbable, wireless nerve stimulator
Biosens Bioelectron. 2024 Mar 16;254:116222. doi: 10.1016/j.bios.2024.116222. Online ahead of print.ABSTRACTMaterials that have the ability to manipulate shapes in response to stimuli such as heat, light, humidity and magnetism offer a means for versatile, sophisticated functions in soft robotics or biomedical implants, while such a reactive transformation has certain drawbacks including high operating temperatures, inherent rigidity and biological hazard. Herein, we introduce biodegradable, self-adhesive, shape-transformable poly (L-lactide-co-ε-caprolactone) (BSS-PLCL) that can be triggered via thermal stimulation near ...
Source: Biosensors and Bioelectronics - March 22, 2024 Category: Biotechnology Authors: Jun Hyeon Lim Won Bae Han Tae-Min Jang Gwan-Jin Ko Jeong-Woong Shin Sungkeun Han Heeseok Kang Chan-Hwi Eom So Jeong Choi Kaveti Rajaram Amay J Bandodkar Woon-Hong Yeo Suk-Won Hwang Source Type: research
Long-term stable pH sensor array with synergistic bilayer structure for 2D real-time mapping in cell culture monitoring
Biosens Bioelectron. 2024 Mar 18;254:116223. doi: 10.1016/j.bios.2024.116223. Online ahead of print.ABSTRACTPursuing accurate, swift, and durable pH sensors is important across numerous fields, encompassing healthcare, environmental surveillance, and agriculture. In particular, the emphasis on real-time pH monitoring during cell cultivation has become increasingly pronounced in the current scientific environment-a crucial element being diligently researched to ensure optimal cell production. Both polyaniline (PANi) and iridium oxide (IrOx) show their worth in pH sensing, yet they come with challenges. Single-PANi-layered p...
Source: Biosensors and Bioelectronics - March 22, 2024 Category: Biotechnology Authors: Jimin Lee Ira Soltis Sayre A Tillery Sung Hoon Lee Hodam Kim Woon-Hong Yeo Source Type: research
On-chip bioluminescence biosensor for the detection of microbial surface contamination
In this study, we introduce a biosensor based on a CMOS image sensor for ATP-mediated chemiluminescence detection. The original lens and IR filter were removed from the CMOS sensor revealing a 12 MP periodic microlens/pixel array on an area of 6.5 mm × 3.6 mm. UltraSnap swabs are used to collect samples from solid surfaces including personal electronic devices, and office and laboratory equipment. Samples mixed with chemiluminescence reagents were placed directly on the surface of the image sensor. Close proximity of the sample to the photodiode array leads to high photon collection efficiency. The population of microorga...
Source: Biosensors and Bioelectronics - March 22, 2024 Category: Biotechnology Authors: Reza Abbasi Meruyert Imanbekova Sebastian Wachsmann-Hogiu Source Type: research
Multifunctional fluorescence probe for simultaneous detection of viscosity, polarity, and ONOO < sup > - < /sup > and its bioimaging in vitro and vivo
Biosens Bioelectron. 2024 Mar 19;254:116233. doi: 10.1016/j.bios.2024.116233. Online ahead of print.ABSTRACTIntracellular microenvironment (viscosity and polarity) and peroxynitrite ions (ONOO-) are involved in maintaining cell morphology, cell function, and signaling so that it is crucial to explore their level changes in vitro and vivo. In this work, we designed and synthesized a mitochondria-targeted fluorescence probe XBL for monitoring the dynamic changes of viscosity, polarity, and ONOO- based on TICT and ICT mechanism. The fluorescence spectra showed obvious changes for polarity at 500 nm as well as ONOO- and viscos...
Source: Biosensors and Bioelectronics - March 22, 2024 Category: Biotechnology Authors: Yuan-Yuan Li Jia-Ling Hu Ji-Rou Wu Yi-Ru Wang Ai-Hong Zhang Yu-Wei Tan Ya-Jing Shang Ting Liang Min Li Ya-Li Meng Yan-Fei Kang Source Type: research
Ultrathin Parylene C-based sensitivity-gain nanoplasmonic sensor integrated on VCSEL for A β < sub > 42 < /sub > detection
This study presents a novel biosensor chip leveraging vertical cavity surface emitting laser (VCSEL) technology, with Parylene C serving as the antibody coupling layer and utilizing a streamlined one-step antibody modification method. Integration of Parylene C enhances chip sensitivity from 34.28 μW/RIU to 40.32 μW/RIU. Moreover, post-testing removal of Parylene C enables chip reusability without significant alteration of results. The sensor demonstrates effective detection of Aβ42, an Alzheimer's biomarker, exhibiting a linear range of 1-200 ng/mL and a detection limit of 0.26 ng/mL. These findings underscore the reusa...
Source: Biosensors and Bioelectronics - March 22, 2024 Category: Biotechnology Authors: Xiaoqing Lv Zhengtai Ma Wenhui Jiang Chengcheng Huang Jun Deng Huan Zhang Pengying Chang Yiyang Xie Source Type: research
Monitoring the electroactive cargo of extracellular vesicles can differentiate various cancer cell lines
Biosens Bioelectron. 2024 Mar 19;254:116224. doi: 10.1016/j.bios.2024.116224. Online ahead of print.ABSTRACTExtracellular vesicles (EVs) are pivotal in cell-to-cell communication due to the array of cargo contained within these vesicles. EVs are considered important biomarkers for identification of disease, however most measurement approaches have focused on monitoring specific surface macromolecular targets. Our study focuses on exploring the electroactive component present within cargo from EVs obtained from various cancer and non-cancer cell lines using a disk carbon fiber microelectrode. Variations in the presence of o...
Source: Biosensors and Bioelectronics - March 21, 2024 Category: Biotechnology Authors: Chloe L Miller Mareike Herrmann David R F Carter Nicholas Turner Priya Samuel Bhavik Anil Patel Source Type: research
Highly ordered nanocavity as photonic-plasmonic-polaritonic resonator for single molecule miRNA SERS detection
Biosens Bioelectron. 2024 Mar 15;254:116231. doi: 10.1016/j.bios.2024.116231. Online ahead of print.ABSTRACTStrong light-matter coupling between molecules and electromagnetic field lead to the formation of hybrid polaritonic states for surface enhanced Raman scattering (SERS) detection. However, owing to the inefficient interaction between zero-point fluctuations of photons/plasmons and molecular electronic transitions, the Raman enhancement is limited in relative low levels. Here, we propose and fabricate a TiOx/Cu2-xSe/R6G nanocavity based photonic-plasmonic-polaritonic resonator for single molecular SERS detection. Thro...
Source: Biosensors and Bioelectronics - March 21, 2024 Category: Biotechnology Authors: Zheng Tian Dawei Xu Shenbo Yang Bing Wang Zhonghai Zhang Source Type: research
A penicillinase-modified poly(N-isopropylacrylamide-co-acrylamide) smart hydrogel biosensor with superior recyclability for sensitive and colorimetric detection of penicillin G
Biosens Bioelectron. 2024 Mar 16;254:116221. doi: 10.1016/j.bios.2024.116221. Online ahead of print.ABSTRACTAntibiotics are widely used for treating bacterial infections. However, excessive or improper use of antibiotics can pose a serious threat to human health and water environments, and thus, developing cost-effective, portable and effective strategies to analyze and detect antibiotics is highly desired. Herein, we reported a responsive photonic hydrogel (RPH)-based optical biosensor (PPNAH) with superior recyclability for sensitive and colorimetric determination of a typical β-lactam antibiotic penicillin G (PG) in wa...
Source: Biosensors and Bioelectronics - March 21, 2024 Category: Biotechnology Authors: Xi Wang Yan-Lin Wang Hai-Rong Yu Xing-Bin Lv Ting Liang Chang-Jing Cheng Source Type: research
Monitoring the electroactive cargo of extracellular vesicles can differentiate various cancer cell lines
Biosens Bioelectron. 2024 Mar 19;254:116224. doi: 10.1016/j.bios.2024.116224. Online ahead of print.ABSTRACTExtracellular vesicles (EVs) are pivotal in cell-to-cell communication due to the array of cargo contained within these vesicles. EVs are considered important biomarkers for identification of disease, however most measurement approaches have focused on monitoring specific surface macromolecular targets. Our study focuses on exploring the electroactive component present within cargo from EVs obtained from various cancer and non-cancer cell lines using a disk carbon fiber microelectrode. Variations in the presence of o...
Source: Biosensors and Bioelectronics - March 21, 2024 Category: Biotechnology Authors: Chloe L Miller Mareike Herrmann David R F Carter Nicholas Turner Priya Samuel Bhavik Anil Patel Source Type: research
Highly ordered nanocavity as photonic-plasmonic-polaritonic resonator for single molecule miRNA SERS detection
Biosens Bioelectron. 2024 Mar 15;254:116231. doi: 10.1016/j.bios.2024.116231. Online ahead of print.ABSTRACTStrong light-matter coupling between molecules and electromagnetic field lead to the formation of hybrid polaritonic states for surface enhanced Raman scattering (SERS) detection. However, owing to the inefficient interaction between zero-point fluctuations of photons/plasmons and molecular electronic transitions, the Raman enhancement is limited in relative low levels. Here, we propose and fabricate a TiOx/Cu2-xSe/R6G nanocavity based photonic-plasmonic-polaritonic resonator for single molecular SERS detection. Thro...
Source: Biosensors and Bioelectronics - March 21, 2024 Category: Biotechnology Authors: Zheng Tian Dawei Xu Shenbo Yang Bing Wang Zhonghai Zhang Source Type: research
A penicillinase-modified poly(N-isopropylacrylamide-co-acrylamide) smart hydrogel biosensor with superior recyclability for sensitive and colorimetric detection of penicillin G
Biosens Bioelectron. 2024 Mar 16;254:116221. doi: 10.1016/j.bios.2024.116221. Online ahead of print.ABSTRACTAntibiotics are widely used for treating bacterial infections. However, excessive or improper use of antibiotics can pose a serious threat to human health and water environments, and thus, developing cost-effective, portable and effective strategies to analyze and detect antibiotics is highly desired. Herein, we reported a responsive photonic hydrogel (RPH)-based optical biosensor (PPNAH) with superior recyclability for sensitive and colorimetric determination of a typical β-lactam antibiotic penicillin G (PG) in wa...
Source: Biosensors and Bioelectronics - March 21, 2024 Category: Biotechnology Authors: Xi Wang Yan-Lin Wang Hai-Rong Yu Xing-Bin Lv Ting Liang Chang-Jing Cheng Source Type: research
Insulin biotrapping using plasmofluidic optical fiber chips: A benchmark
This study lays the foundation for the detection of punctual molecular interactions and opens the way towards the detection of spatially and temporally localized events on the surface of optical probes.PMID:38507927 | DOI:10.1016/j.bios.2024.116189 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - March 20, 2024 Category: Biotechnology Authors: M édéric Loyez Hadrien Fasseaux Maxime Lobry Ruddy Wattiez Christophe Caucheteur Source Type: research
A generalizable sensing platform based on molecularly imprinted polymer-aptamer double recognition and nanoenzyme assisted photoelectrochemical-colorimetric dual-mode detection
In this study, a generalizable photoelectrochemical-colorimetric dual-mode sensing platform was constructed based on the synergistic effects of a molecularly imprinted polymer (MIP)-aptamer sandwich structure and nanoenzymes. The MIP functionalized peroxidase-like Fe3O4 (Fe3O4@MIPs) and alkaline phosphatase mimic Zr-MOF labeled aptamer (Zr-mof@Apt) were used as the recognition elements. By selectively accumulating dibutyl phthalate (DBP), a small molecule target model, on Fe3O4@MIPs, the formation of Zr-MOF@Apt-DBP- Fe3O4@MIPs sandwich structure was triggered. Fe3O4@MIPs oxidized TMB to form blue-colored oxTMB. However, up...
Source: Biosensors and Bioelectronics - March 20, 2024 Category: Biotechnology Authors: Ying-Zhuo Shen Wen Zheng Xie Zheng Wang Kang Ping Ning Zheng Ping Ji Hong Bo Li Xiao-Ya Hu Cheng Ma Xu Qin Source Type: research
Biosensors; a novel concept in real-time detection of autophagy
Biosens Bioelectron. 2024 Mar 16;254:116204. doi: 10.1016/j.bios.2024.116204. Online ahead of print.ABSTRACTAutophagy is an early-stage response with self-degradation properties against several insulting conditions. To date, the critical role of autophagy has been well-documented in physiological and pathological conditions. This process involves various signaling and functional biomolecules, which are involved in different steps of the autophagic response. During recent decades, a range of biochemical analyses, chemical assays, and varied imaging techniques have been used for monitoring this pathway. Due to the complexity...
Source: Biosensors and Bioelectronics - March 20, 2024 Category: Biotechnology Authors: Hassan Nasrollahpour Arezoo Mirzaie Maryam Sharifi Aysa Rezabakhsh Balal Khalilzadeh Reza Rahbarghazi Hadi Yousefi Daniel J Klionsky Source Type: research
