Development of highly sensitive dual-enhanced fluorescence quenching immunochromatographic test strips based on Pt nanoprobes
Biosens Bioelectron. 2024 Mar 6;254:116195. doi: 10.1016/j.bios.2024.116195. Online ahead of print.ABSTRACTThe fluorescence-quenching method is crucial in vitro analysis, particularly for immunochromatographic test strips (ICTs) using noble metal nanoparticles as probes. However, ICTs still fall short in meeting the requirements for the detection of traces biomarkers due to the noble metal nanoparticles can only quench fluorescence of the dyes within a confined distance. Interestingly, noble metal nanoparticles, such as Pt NPs cannot only perform fluorescence-quenching ability based on the Förster resonance energy transfe...
Source: Biosensors and Bioelectronics - March 13, 2024 Category: Biotechnology Authors: Jiashuo Xu Shuang Zhao Qiuting Zhang Xu Huang Kang Du Jinzhi Wang Jiaxun Wang Cheng Chen Bingbo Zhang Jin Chang Xiaoqun Gong Source Type: research
Polymerized carbon dots with high electrochemiluminescence efficiency and long wavelength ECL emission for ultrasensitive detection of MicroRNA-222
Biosens Bioelectron. 2024 Mar 5;254:116193. doi: 10.1016/j.bios.2024.116193. Online ahead of print.ABSTRACTHerein, a new electrochemiluminescence (ECL) biosensor was constructed with highly efficient polymerized carbon dots (PCDs) as ECL emitter and the improved localized catalytic hairpin assembly (L-CHA) as signal amplifier for ultrasensitive detection of microRNA-222 (miRNA-222). Impressively, compared to the traditional carbon dots with inefficient blue region ECL emission, PCDs with N, O co-dope and large conjugated π-system showed high electrical conductivity, narrow band gap and strong radiative transition, which c...
Source: Biosensors and Bioelectronics - March 13, 2024 Category: Biotechnology Authors: Shuang Xiao Yu-Ting Yang Yi-Fei Chen Jia-Li Liu Ya-Qin Chai Ruo Yuan Source Type: research
One-step construction of multiplexed enzymatic biosensors using light-addressable electrochemistry on a single silicon photoelectrode
Biosens Bioelectron. 2024 Mar 6;253:116194. doi: 10.1016/j.bios.2024.116194. Online ahead of print.ABSTRACTThe multiplexed detection of metabolites in parallel within a single biosensor plate is sufficiently valuable but also challenging. Herein, we combine the inherent light addressability of silicon with the high selectivity of enzymes, for the construction of multiplexed photoelectrochemical enzymatic biosensors. To conduct a stable electrochemistry and reagentless biosensing on silicon, a new strategy involving the immobilization of both redox mediators and enzymes using an amide bond-based hydrogel membrane was propos...
Source: Biosensors and Bioelectronics - March 11, 2024 Category: Biotechnology Authors: Qiaoyu Yang Jiaming Liao Luyao Feng Sen Wang Zhibin Zhao Jian Wang Yazhong Bu Jian Zhuang De-Wen Zhang Source Type: research
Rapid and accurate SERS assay of disease-related nucleic acids based on isothermal cascade signal amplifications of CRISPR/Cas13a system and catalytic hairpin assembly
Biosens Bioelectron. 2024 Mar 6;253:116196. doi: 10.1016/j.bios.2024.116196. Online ahead of print.ABSTRACTDeveloping rapid, accurate and convenient nucleic acid diagnostic techniques is essential for the prevention and control of contagious diseases that are prone to gene mutations and may have homologous sequences, especially emerging infectious diseases such as the SARS-CoV-2 pandemic. Herein, a one-pot SERS assay integrating isothermal cascade signal amplification strategy (i.e., CRISPR/Cas13a system (Cas13a) and catalytic hairpin assembly (CHA), Cas13a-CHA) and SERS-active silver nanorods (AgNRs) sensing chips was pro...
Source: Biosensors and Bioelectronics - March 11, 2024 Category: Biotechnology Authors: Jingjing Zhang Zhilong Chen Huiming Lv Jing Liang Chenlong Yan Chunyuan Song Lianhui Wang Source Type: research
One-step construction of multiplexed enzymatic biosensors using light-addressable electrochemistry on a single silicon photoelectrode
Biosens Bioelectron. 2024 Mar 6;253:116194. doi: 10.1016/j.bios.2024.116194. Online ahead of print.ABSTRACTThe multiplexed detection of metabolites in parallel within a single biosensor plate is sufficiently valuable but also challenging. Herein, we combine the inherent light addressability of silicon with the high selectivity of enzymes, for the construction of multiplexed photoelectrochemical enzymatic biosensors. To conduct a stable electrochemistry and reagentless biosensing on silicon, a new strategy involving the immobilization of both redox mediators and enzymes using an amide bond-based hydrogel membrane was propos...
Source: Biosensors and Bioelectronics - March 11, 2024 Category: Biotechnology Authors: Qiaoyu Yang Jiaming Liao Luyao Feng Sen Wang Zhibin Zhao Jian Wang Yazhong Bu Jian Zhuang De-Wen Zhang Source Type: research
Rapid and accurate SERS assay of disease-related nucleic acids based on isothermal cascade signal amplifications of CRISPR/Cas13a system and catalytic hairpin assembly
Biosens Bioelectron. 2024 Mar 6;253:116196. doi: 10.1016/j.bios.2024.116196. Online ahead of print.ABSTRACTDeveloping rapid, accurate and convenient nucleic acid diagnostic techniques is essential for the prevention and control of contagious diseases that are prone to gene mutations and may have homologous sequences, especially emerging infectious diseases such as the SARS-CoV-2 pandemic. Herein, a one-pot SERS assay integrating isothermal cascade signal amplification strategy (i.e., CRISPR/Cas13a system (Cas13a) and catalytic hairpin assembly (CHA), Cas13a-CHA) and SERS-active silver nanorods (AgNRs) sensing chips was pro...
Source: Biosensors and Bioelectronics - March 11, 2024 Category: Biotechnology Authors: Jingjing Zhang Zhilong Chen Huiming Lv Jing Liang Chenlong Yan Chunyuan Song Lianhui Wang Source Type: research
Self-powered optical fiber biosensor integrated with enzymes for non-invasive glucose sensing
Biosens Bioelectron. 2024 Mar 5;253:116191. doi: 10.1016/j.bios.2024.116191. Online ahead of print.ABSTRACTTo alleviate the discomfort associated with frequent blood glucose detection in diabetic patients, a novel non-invasive tear glucose biosensor has been developed. This involved the design and preparation of a photoelectrochemical probe based on an optical fiber and biological enzymes. One end of the optical fiber connects to a light source, acting as an energy source and imparting, self-powered capability to the biosensor. The opposite end is loaded with nanomaterials and glucose oxidase, designed for insertion into t...
Source: Biosensors and Bioelectronics - March 9, 2024 Category: Biotechnology Authors: Xingyue Wen Xinghua Yang Zhongxuan Ge Hongyu Ma Rui Wang Fengjun Tian Pingping Teng Shuai Gao Kang Li Bo Zhang Sivagunalan Sivanathan Source Type: research
Ultrasimple size encoded microfluidic chip for rapid simultaneous multiplex detection of DNA sequences
In this study, an ultrasimple size encoding method is proposed for multiplex detection using a wedge-shaped microfluidic chip. Driving by negative pressure, microparticles are naturally arranged in distinct stripes based on their sizes within the chip. This size encoding method demonstrates a high level of precision, allowing for accuracy in distinguishing 3-5 sizes of microparticles with a remarkable accuracy rate of up to 99%, even the microparticles with a size difference as small as 0.5 μm. The entire size encoding process is completed in less than 5 min, making it ultrasimple, reliable, and easy to operate. To evalua...
Source: Biosensors and Bioelectronics - March 9, 2024 Category: Biotechnology Authors: Man Tang Kuan-Jie Zhu Wei Sun Xinyue Yuan Zhipeng Wang Ruyi Zhang Zhao Ai Kan Liu Source Type: research
Self-powered optical fiber biosensor integrated with enzymes for non-invasive glucose sensing
Biosens Bioelectron. 2024 Mar 5;253:116191. doi: 10.1016/j.bios.2024.116191. Online ahead of print.ABSTRACTTo alleviate the discomfort associated with frequent blood glucose detection in diabetic patients, a novel non-invasive tear glucose biosensor has been developed. This involved the design and preparation of a photoelectrochemical probe based on an optical fiber and biological enzymes. One end of the optical fiber connects to a light source, acting as an energy source and imparting, self-powered capability to the biosensor. The opposite end is loaded with nanomaterials and glucose oxidase, designed for insertion into t...
Source: Biosensors and Bioelectronics - March 9, 2024 Category: Biotechnology Authors: Xingyue Wen Xinghua Yang Zhongxuan Ge Hongyu Ma Rui Wang Fengjun Tian Pingping Teng Shuai Gao Kang Li Bo Zhang Sivagunalan Sivanathan Source Type: research
Ultrasimple size encoded microfluidic chip for rapid simultaneous multiplex detection of DNA sequences
In this study, an ultrasimple size encoding method is proposed for multiplex detection using a wedge-shaped microfluidic chip. Driving by negative pressure, microparticles are naturally arranged in distinct stripes based on their sizes within the chip. This size encoding method demonstrates a high level of precision, allowing for accuracy in distinguishing 3-5 sizes of microparticles with a remarkable accuracy rate of up to 99%, even the microparticles with a size difference as small as 0.5 μm. The entire size encoding process is completed in less than 5 min, making it ultrasimple, reliable, and easy to operate. To evalua...
Source: Biosensors and Bioelectronics - March 9, 2024 Category: Biotechnology Authors: Man Tang Kuan-Jie Zhu Wei Sun Xinyue Yuan Zhipeng Wang Ruyi Zhang Zhao Ai Kan Liu Source Type: research
Self-powered optical fiber biosensor integrated with enzymes for non-invasive glucose sensing
Biosens Bioelectron. 2024 Mar 5;253:116191. doi: 10.1016/j.bios.2024.116191. Online ahead of print.ABSTRACTTo alleviate the discomfort associated with frequent blood glucose detection in diabetic patients, a novel non-invasive tear glucose biosensor has been developed. This involved the design and preparation of a photoelectrochemical probe based on an optical fiber and biological enzymes. One end of the optical fiber connects to a light source, acting as an energy source and imparting, self-powered capability to the biosensor. The opposite end is loaded with nanomaterials and glucose oxidase, designed for insertion into t...
Source: Biosensors and Bioelectronics - March 9, 2024 Category: Biotechnology Authors: Xingyue Wen Xinghua Yang Zhongxuan Ge Hongyu Ma Rui Wang Fengjun Tian Pingping Teng Shuai Gao Kang Li Bo Zhang Sivagunalan Sivanathan Source Type: research
Ultrasimple size encoded microfluidic chip for rapid simultaneous multiplex detection of DNA sequences
In this study, an ultrasimple size encoding method is proposed for multiplex detection using a wedge-shaped microfluidic chip. Driving by negative pressure, microparticles are naturally arranged in distinct stripes based on their sizes within the chip. This size encoding method demonstrates a high level of precision, allowing for accuracy in distinguishing 3-5 sizes of microparticles with a remarkable accuracy rate of up to 99%, even the microparticles with a size difference as small as 0.5 μm. The entire size encoding process is completed in less than 5 min, making it ultrasimple, reliable, and easy to operate. To evalua...
Source: Biosensors and Bioelectronics - March 9, 2024 Category: Biotechnology Authors: Man Tang Kuan-Jie Zhu Wei Sun Xinyue Yuan Zhipeng Wang Ruyi Zhang Zhao Ai Kan Liu Source Type: research
Development of a point-of-care colorimetric metabolomic sensor platform
Biosens Bioelectron. 2024 Mar 4;253:116186. doi: 10.1016/j.bios.2024.116186. Online ahead of print.ABSTRACTMetabolomics is the large-scale study of small molecule metabolites within a biological system. It has applications in measuring dietary intake, predicting heart disease risk, and diagnosing cancer. Metabolites are often measured using high-end analytical tools such as mass spectrometers or large spectrophotometers. However, due to their size, cost, and need for skilled operators, using such equipment at the bedside is not practical. To address this issue, we have developed a low-cost, portable, optical color sensor p...
Source: Biosensors and Bioelectronics - March 8, 2024 Category: Biotechnology Authors: Songtian Bai Pablo Gonzalez-Vasquez Claudia Torres-Calzada Scott MacKay James Cook Yeganeh Khaniani Gareth Davies Upasana Singh Prashanthi Kovur Jie Chen David S Wishart Source Type: research
Electrochemical DNA-based sensors for measuring cell-generated forces
Biosens Bioelectron. 2024 Mar 1;253:116185. doi: 10.1016/j.bios.2024.116185. Online ahead of print.ABSTRACTMechanical forces play an important role in cellular communication and signaling. We developed in this study novel electrochemical DNA-based force sensors for measuring cell-generated adhesion forces. Two types of DNA probes, i.e., tension gauge tether and DNA hairpin, were constructed on the surface of a smartphone-based electrochemical device to detect piconewton-scale cellular forces at tunable levels. Upon experiencing cellular tension, the unfolding of DNA probes induces the separation of redox reporters from the...
Source: Biosensors and Bioelectronics - March 8, 2024 Category: Biotechnology Authors: Mahmoud Amouzadeh Tabrizi Priyanka Bhattacharyya Ru Zheng Mingxu You Source Type: research
A novel electrochemical strategy to detect hydrogen peroxide by utilizing peroxidase-mimicking activity of cerium oxide/graphene oxide nanocomposites
Biosens Bioelectron. 2024 Feb 22;253:116161. doi: 10.1016/j.bios.2024.116161. Online ahead of print.ABSTRACTWe herein describe a novel electrochemical strategy to detect hydrogen peroxide (H2O2) by utilizing the peroxidase-mimicking activity of cerium oxide nanoparticles (CeO2 NP) and reduced graphene oxide (rGO). Particularly, CeO2 NP/rGO nanocomposites were deposited on the commercial electrode by a very convenient and direct electrochemical reduction of graphene oxide. Due to the peroxidase-mimicking activity of CeO2 NP and the outstanding electrochemical properties of reduced graphene oxide, the reduction current of H2...
Source: Biosensors and Bioelectronics - March 8, 2024 Category: Biotechnology Authors: Sunghyeon Kim Shin Chan Kang Sang Mo Lee Jinhwan Lee Youngran Cho Yeonjin Shim Hyun Gyu Park Source Type: research
