Paper-based loop-mediated isothermal amplification and CRISPR integrated platform for on-site nucleic acid testing of pathogens
We report the development and initial validation of a paper-based nucleic acid testing platform that integrates Loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPR) technology, referred to as PLACID (Paper-based LAMP-CRISPR Integrated Diagnostics). LAMP eliminates the need for thermal cycling, resulting in simplified instrumentation, and the CRISPR-associated protein (Cas 12a) system eliminates false positive signals from LAMP products, resulting in highly selective and sensitive assays. We optimized the assay to perform both amplification and detection entir...
Source: Biosensors and Bioelectronics - April 23, 2024 Category: Biotechnology Authors: Anindita Sen Manaswini Masetty Sasanka Weerakoon Calum Morris Jagjit S Yadav Senu Apewokin Jennifer Trannguyen Murray Broom Aashish Priye Source Type: research
Recent advances in implantable sensors and electronics using printable materials for advanced healthcare
Biosens Bioelectron. 2024 Apr 17;257:116302. doi: 10.1016/j.bios.2024.116302. Online ahead of print.ABSTRACTThis review article focuses on the recent printing technological progress in healthcare, underscoring the significant potential of implantable devices across diverse applications. Printing technologies have widespread use in developing health monitoring devices, diagnostic systems, and surgical devices. Recent years have witnessed remarkable progress in fabricating low-profile implantable devices, driven by advancements in printing technologies and nanomaterials. The importance of implantable biosensors and bioelectr...
Source: Biosensors and Bioelectronics - April 22, 2024 Category: Biotechnology Authors: Seunghyeb Ban Haran Lee Jiehao Chen Hee-Seok Kim Yuhang Hu Seong J Cho Woon-Hong Yeo Source Type: research
Rational design of nanozyme with integrated sample pretreatment for colorimetric biosensing
Biosens Bioelectron. 2024 Apr 17;257:116310. doi: 10.1016/j.bios.2024.116310. Online ahead of print.ABSTRACTNanozymes have been widely used in the field of biosensing owing to their high stability, low cost, adjustable catalytic activity, and convenient modification. However, achieving high selectivity and sensitivity simultaneously in nanozyme-based colorimetric sensing remains a major challenge. Nanozymes are nanomaterials with enzyme-simulating activity that are often used as solid-phase adsorbents for sample pretreatment. Our design strategy integrated sample pretreatment function into the nanozyme through separation a...
Source: Biosensors and Bioelectronics - April 21, 2024 Category: Biotechnology Authors: Jia Wu Ling Liang Shuishi Li Yuan Qin Shulin Zhao Fanggui Ye Source Type: research
Highly sensitive humidity sensor based on composite film of partially reduced graphene oxide and bacterial cellulose
In this study, a humidity sensing composite film with high sensitivity and short response time was made by using the mixture of graphene oxide (GO) and bacterial cellulose (BC) with simple dry film-forming method. L-ascorbic acid was used as reducing agent to reduce GO and improve the conductivity of GO/BC composite film (BG). The influence of different BC contents and the different reduction degree on the resistance change rate of composite film was investigated in details. The maximum resistance change rate of partially reduced BG humidity sensitive composite film reached up to 94%, and the response and recovery time wer...
Source: Biosensors and Bioelectronics - April 21, 2024 Category: Biotechnology Authors: Jingyi Song Mingshuai Fan Rui Zhang Meijie Qu Ping Tang Hai Wang Yuezhen Bin Source Type: research
An ingestible near-infrared fluorescence capsule endoscopy for specific gastrointestinal diagnoses
Biosens Bioelectron. 2024 Mar 24;257:116209. doi: 10.1016/j.bios.2024.116209. Online ahead of print.ABSTRACTEarly diagnosis of gastrointestinal (GI) diseases is important to effectively prevent carcinogenesis. Capsule endoscopy (CE) can address the pain caused by wired endoscopy in GI diagnosis. However, existing CE approaches have difficulty effectively diagnosing lesions that do not exhibit obvious morphological changes. In addition, the current CE cannot achieve wireless energy supply and attitude control at the same time. Here, we successfully developed a novel near-infrared fluorescence capsule endoscopy (NIFCE) that ...
Source: Biosensors and Bioelectronics - April 19, 2024 Category: Biotechnology Authors: Cheng Zhou Jinlei Jiang Songwei Huang Junhao Wang Xinyuan Cui Weicheng Wang Mingrui Chen Jiawei Peng Nanqing Shi Bensong Wang Amin Zhang Qian Zhang Qichao Li Shengsheng Cui Shenghao Xue Wei Wang Ning Tang Daxiang Cui Source Type: research
Editorial note: Low temperature synthesis of carbon dots in microfluidic chip and their application for sensing cefquinome residues in milk [Biosens. Bioelectron. 228 (2023) 115187]
Biosens Bioelectron. 2024 Apr 18:116093. doi: 10.1016/j.bios.2024.116093. Online ahead of print.NO ABSTRACTPMID:38641457 | DOI:10.1016/j.bios.2024.116093 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - April 19, 2024 Category: Biotechnology Authors: Guangming Li Chen Liu Xingcai Zhang Peng Zhai Xinyi Lai Wenxiao Jiang Source Type: research
High-throughput visualization mutation screening technology to enhance the specificity of CadR based whole-cell cadmium biosensor
In this study, a strategy of Adjacent Site Saturation Mutation (ASSM) was designed to improve the specificity of transcription factor CadR, which acted as the recognition element and determined the specificity of whole cell Cd2+ biosensors. A specific saturated library was constructed using the strategy of adjacent mutation. After two rounds of high-throughput visual screening, a whole-cell biosensor with good response to Cd2+, and with significant weakened Hg2+ interference was obtained. The optimized whole-cell biosensor showed a linear dynamic concentration range from 500 nM to 100 μM, a detection limit of 0.079 μM, a...
Source: Biosensors and Bioelectronics - April 18, 2024 Category: Biotechnology Authors: Mingzhang Guo Shijing Chen Hongfei Su Xiaolin Chen Huilin Liu Baoguo Sun Source Type: research
A rapid and sensitive one-pot platform integrating fluorogenic RNA aptamers and CRISPR-Cas13a for visual detection of monkeypox virus
In this study, we utilized a cost-effective and stable fluorogenic RNA aptamer (Mango III), specifically binding and illuminating the fluorophore TO3-3 PEG-Biotin Fluorophore (TO3), as a reporter for Cas13a trans-cleavage activity. We propose a comprehensive strategy integrating RNA aptamer, recombinase-aided amplification (RAA), and CRISPR-Cas13a systems for the molecular detection of MPXV target. Leveraging the inherent collateral cleavage properties of the Cas13a system, we established high-sensitivity and specificity assays to distinguish MPXV from other Orthopoxviruses (OPVs). A streamlined one-pot protocol was develo...
Source: Biosensors and Bioelectronics - April 18, 2024 Category: Biotechnology Authors: Xiao Wang Xiaobao Deng Yidun Zhang Weiyi Dong Qiao Rao Qingmei Huang Fei Tang Rong Shen Hongzhi Xu Zhen Jin Youzhi Tang Dan Du Source Type: research
Construction of AlGaN/GaN high-electron-mobility transistor-based biosensor for ultrasensitive detection of SARS-CoV-2 spike proteins and virions
Biosens Bioelectron. 2024 Mar 15;257:116171. doi: 10.1016/j.bios.2024.116171. Online ahead of print.ABSTRACTThe COVID-19 pandemic has highlighted the need for rapid and sensitive detection of SARS-CoV-2. Here, we report an ultrasensitive SARS-CoV-2 immunosensor by integration of an AlGaN/GaN high-electron-mobility transistor (HEMT) and anti-SARS-CoV-2 spike protein antibody. The AlGaN/GaN HEMT immunosensor has demonstrated the capability to detect SARS-CoV-2 spike proteins at an impressively low concentration of 10-22 M. The sensor was also applied to pseudoviruses and SARS-CoV-2 ΔN virions that display the Spike proteins...
Source: Biosensors and Bioelectronics - April 18, 2024 Category: Biotechnology Authors: Chenyang Yang Jianwen Sun Yulong Zhang Jingya Tang Zizheng Liu Teng Zhan Dian-Bing Wang Guoqi Zhang Zewen Liu Xian-En Zhang Source Type: research
Adaptively resettable microfluidic patch for sweat rate and electrolytes detection
Biosens Bioelectron. 2024 Apr 16;257:116299. doi: 10.1016/j.bios.2024.116299. Online ahead of print.ABSTRACTSkin-interfaced microfluidic patch has become a reliable device for sweat collection and analysis. However, the intractable problems of emptying the microchannel for reuse, and the channel's volumetric capacity limited by the size of the patch, directly hinder the practical application of sweat sensors. Herein, we report an adaptively resettable microfluidic sweat patch (Art-Sweat patch) capable of continuously monitoring both sweat rate (0.2-4.0 μL min-1) and total ionic charge concentration (10-200 mmol L-1). We d...
Source: Biosensors and Bioelectronics - April 18, 2024 Category: Biotechnology Authors: Mengyuan Liu Siyuan Liu Tong Zhang Dengfeng Zhou Lianhui Li Qiang Gao Yujie Liu Changlei Ge Yongfeng Wang Mingxu Wang Feng Wen Zuoping Xiong Zhen Zhou Shuqi Wang Ting Zhang Source Type: research
High-throughput visualization mutation screening technology to enhance the specificity of CadR based whole-cell cadmium biosensor
In this study, a strategy of Adjacent Site Saturation Mutation (ASSM) was designed to improve the specificity of transcription factor CadR, which acted as the recognition element and determined the specificity of whole cell Cd2+ biosensors. A specific saturated library was constructed using the strategy of adjacent mutation. After two rounds of high-throughput visual screening, a whole-cell biosensor with good response to Cd2+, and with significant weakened Hg2+ interference was obtained. The optimized whole-cell biosensor showed a linear dynamic concentration range from 500 nM to 100 μM, a detection limit of 0.079 μM, a...
Source: Biosensors and Bioelectronics - April 18, 2024 Category: Biotechnology Authors: Mingzhang Guo Shijing Chen Hongfei Su Xiaolin Chen Huilin Liu Baoguo Sun Source Type: research
A rapid and sensitive one-pot platform integrating fluorogenic RNA aptamers and CRISPR-Cas13a for visual detection of monkeypox virus
In this study, we utilized a cost-effective and stable fluorogenic RNA aptamer (Mango III), specifically binding and illuminating the fluorophore TO3-3 PEG-Biotin Fluorophore (TO3), as a reporter for Cas13a trans-cleavage activity. We propose a comprehensive strategy integrating RNA aptamer, recombinase-aided amplification (RAA), and CRISPR-Cas13a systems for the molecular detection of MPXV target. Leveraging the inherent collateral cleavage properties of the Cas13a system, we established high-sensitivity and specificity assays to distinguish MPXV from other Orthopoxviruses (OPVs). A streamlined one-pot protocol was develo...
Source: Biosensors and Bioelectronics - April 18, 2024 Category: Biotechnology Authors: Xiao Wang Xiaobao Deng Yidun Zhang Weiyi Dong Qiao Rao Qingmei Huang Fei Tang Rong Shen Hongzhi Xu Zhen Jin Youzhi Tang Dan Du Source Type: research
Construction of AlGaN/GaN high-electron-mobility transistor-based biosensor for ultrasensitive detection of SARS-CoV-2 spike proteins and virions
Biosens Bioelectron. 2024 Mar 15;257:116171. doi: 10.1016/j.bios.2024.116171. Online ahead of print.ABSTRACTThe COVID-19 pandemic has highlighted the need for rapid and sensitive detection of SARS-CoV-2. Here, we report an ultrasensitive SARS-CoV-2 immunosensor by integration of an AlGaN/GaN high-electron-mobility transistor (HEMT) and anti-SARS-CoV-2 spike protein antibody. The AlGaN/GaN HEMT immunosensor has demonstrated the capability to detect SARS-CoV-2 spike proteins at an impressively low concentration of 10-22 M. The sensor was also applied to pseudoviruses and SARS-CoV-2 ΔN virions that display the Spike proteins...
Source: Biosensors and Bioelectronics - April 18, 2024 Category: Biotechnology Authors: Chenyang Yang Jianwen Sun Yulong Zhang Jingya Tang Zizheng Liu Teng Zhan Dian-Bing Wang Guoqi Zhang Zewen Liu Xian-En Zhang Source Type: research
Adaptively resettable microfluidic patch for sweat rate and electrolytes detection
Biosens Bioelectron. 2024 Apr 16;257:116299. doi: 10.1016/j.bios.2024.116299. Online ahead of print.ABSTRACTSkin-interfaced microfluidic patch has become a reliable device for sweat collection and analysis. However, the intractable problems of emptying the microchannel for reuse, and the channel's volumetric capacity limited by the size of the patch, directly hinder the practical application of sweat sensors. Herein, we report an adaptively resettable microfluidic sweat patch (Art-Sweat patch) capable of continuously monitoring both sweat rate (0.2-4.0 μL min-1) and total ionic charge concentration (10-200 mmol L-1). We d...
Source: Biosensors and Bioelectronics - April 18, 2024 Category: Biotechnology Authors: Mengyuan Liu Siyuan Liu Tong Zhang Dengfeng Zhou Lianhui Li Qiang Gao Yujie Liu Changlei Ge Yongfeng Wang Mingxu Wang Feng Wen Zuoping Xiong Zhen Zhou Shuqi Wang Ting Zhang Source Type: research
Biosensors for psychiatric biomarkers in mental health monitoring
Biosens Bioelectron. 2024 Mar 29;256:116242. doi: 10.1016/j.bios.2024.116242. Online ahead of print.ABSTRACTPsychiatric disorders are associated with serve disturbances in cognition, emotional control, and/or behavior regulation, yet few routine clinical tools are available for the real-time evaluation and early-stage diagnosis of mental health. Abnormal levels of relevant biomarkers may imply biological, neurological, and developmental dysfunctions of psychiatric patients. Exploring biosensors that can provide rapid, in-situ, and real-time monitoring of psychiatric biomarkers is therefore vital for prevention, diagnosis, ...
Source: Biosensors and Bioelectronics - April 17, 2024 Category: Biotechnology Authors: Lin Wang Yubing Hu Nan Jiang Ali K Yetisen Source Type: research
