A "dual-key-and-lock" platform for distinguishing autophagy during neuroinflammation
Biosens Bioelectron. 2024 Apr 27;258:116344. doi: 10.1016/j.bios.2024.116344. Online ahead of print.ABSTRACTAutophagy is an essential degradative process that governs the renewal of organelle and maintains the homeostasis of cellular microenvironment. Its dysregulation has been demonstrated to be an indicator for neuroinflammation. To elucidate the interrelationship between neuroinflammation and autophagy, optical probes are ideal tools as they offer a number of advantages such as high spatiotemporal resolution and non-invasive sensing, which help to visualize the physiological and pathological functions of interested anal...
Source: Biosensors and Bioelectronics - May 2, 2024 Category: Biotechnology Authors: Shuyang Zhai Wei Hu Zhihong Liu Yingbin Liu Source Type: research
Sensing patches for biomarker identification in skin-derived biofluids
Biosens Bioelectron. 2024 Apr 22;258:116326. doi: 10.1016/j.bios.2024.116326. Online ahead of print.ABSTRACTIn conventional clinical disease diagnosis and screening based on biomarker detection, most analysis samples are collected from serum, blood. However, these invasive collection methods require specific instruments, professionals, and may lead to infection risks. Additionally, the diagnosis process suffers from untimely results. The identification of skin-related biomarkers plays an unprecedented role in early disease diagnosis. More importantly, these skin-mediated approaches for collecting biomarker-containing biofl...
Source: Biosensors and Bioelectronics - May 2, 2024 Category: Biotechnology Authors: Chen Wei Danni Fu Tianyue Ma Mo Chen Fangling Wang Guojun Chen Zejun Wang Source Type: research
Development of a thermotaxis and rheotaxis microfluidic device for motile spermatozoa sorting
This study introduces a groundbreaking development in the form of a thermotaxis and rheotaxis microfluidic (TRMC) device designed for efficient motile spermatozoa sorting within a short 15-min timeframe. The TRMC device mimics the natural sperm sorting mechanism of the oviduct, selecting spermatozoa with superior motility and DNA integrity. The experimental outcomes demonstrate a remarkable enhancement in the percentage of progressive spermatozoa following sorting, soaring from 3.90% to an impressive 96.11% when subjected to a temperature decrease from 38 °C to 35 °C. Notably, sperm motility exhibited a substantial 69% i...
Source: Biosensors and Bioelectronics - May 2, 2024 Category: Biotechnology Authors: Teng-Kuan Huang Chung-Hsien Huang Pei-An Chen Ching Hung Chen Farn Lu Wen-Ju Yang Jack Yu Jen Huang Bor-Ran Li Source Type: research
A "dual-key-and-lock" platform for distinguishing autophagy during neuroinflammation
Biosens Bioelectron. 2024 Apr 27;258:116344. doi: 10.1016/j.bios.2024.116344. Online ahead of print.ABSTRACTAutophagy is an essential degradative process that governs the renewal of organelle and maintains the homeostasis of cellular microenvironment. Its dysregulation has been demonstrated to be an indicator for neuroinflammation. To elucidate the interrelationship between neuroinflammation and autophagy, optical probes are ideal tools as they offer a number of advantages such as high spatiotemporal resolution and non-invasive sensing, which help to visualize the physiological and pathological functions of interested anal...
Source: Biosensors and Bioelectronics - May 2, 2024 Category: Biotechnology Authors: Shuyang Zhai Wei Hu Zhihong Liu Yingbin Liu Source Type: research
Homochiral light-sensitive metal-organic framework photoelectrochemical gated transistor for enantioselective discrimination of monosaccharides
In this study, a light-sensitive organic photoelectrochemical transistor (OPECT) with homochiral character is developed for enantiomer discrimination. Demonstrated with the discrimination of glucose enantiomers, the photoelectrochemically active gate electrode is prepared by integrating Au nanoparticles (AuNPs) and a chiral Cu(II)-metal-organic framework (c-CuMOF) onto TiO2 nanotube arrays (TNT). The captured glucose enantiomers are oxidized to hydrogen peroxide (H2O2) by the oxidase-mimicking AuNPs-loaded c-CuMOF. Based on the confinement effect of the mesopocket structure of the c-CuMOF and the remarkable charge transfer...
Source: Biosensors and Bioelectronics - May 1, 2024 Category: Biotechnology Authors: Jian-Hong Zhu Haiquan Wang Junli Guo Junjian Zhao Zhida Gao Yan-Yan Song Chenxi Zhao Source Type: research
Softening implantable bioelectronics: Material designs, applications, and future directions
This article reviews recent research and development in softening materials and designs for implantable bioelectronics. Examples featuring tissue-penetrating and conformal softening devices highlight the promising potential of these approaches in biomedical applications. A concluding section delves into current challenges and outlines future directions for softening implantable device technologies, underscoring their pivotal role in propelling the evolution of next-generation bioelectronics.PMID:38692223 | DOI:10.1016/j.bios.2024.116328 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - May 1, 2024 Category: Biotechnology Authors: Subin Oh Simok Lee Sung Woo Kim Choong Yeon Kim Eun Young Jeong Juhyun Lee Do A Kwon Jae-Woong Jeong Source Type: research
Integrated molybdenum single atom array sensors with multichannels for nitrite detection in foods
In this study, an integrated array sensor for NO2- detection is developed based on molybdenum single atom material (IMSMo-SAC) using high-resolution electrohydrodynamic (EHD) printing technology. The sensor comprises three components: a printed electrode array, multichannels designed on polydimethylsiloxane (PDMS) and an electronic signal process device with bluetooth. By utilizing Mo-SAC to facilitate electron transfer during the redox reaction, rapid and efficient detection of NO2- can be achieved. The sensor has a wide linear range of 0.1 μM-107.8 mM, a low detection limit of 33 nM and a high sensitivity of 0.637 mA-1m...
Source: Biosensors and Bioelectronics - May 1, 2024 Category: Biotechnology Authors: Mei Ding Kangling Tian Jingwen Wang Yuhang Liu Guangxuan Hu Yan Zheng Shaohui Lei Jiayue Sun Hong Bin Yang Fang Xin Hu Source Type: research
Development of a CRISPR/Cas12a-mediated aptasensor for Mpox virus antigen detection
Biosens Bioelectron. 2024 Apr 24;257:116313. doi: 10.1016/j.bios.2024.116313. Online ahead of print.ABSTRACTThe emergence and rapid spread of Mpox (formerly monkeypox) have caused significant societal challenges. Adequate and appropriate diagnostics procedures are an urgent necessity. Herein, we discover a pair of aptamers through the systematic evolution of ligands by exponential enrichment (SELEX) that exhibit high affinity and bind to different sites towards the A29 protein of the Mpox virus. Subsequently, we propose a facile, sensitive, convenient CRISPR/Cas12a-mediated aptasensor for detecting the A29 antigen. The pro...
Source: Biosensors and Bioelectronics - April 30, 2024 Category: Biotechnology Authors: Cong Han Qirui Liu Xuantong Luo Jian Zhao Zheng Zhang Jiaxuan He Feng Ge Wei Ding Zhaofeng Luo Chao Jia Liyun Zhang Source Type: research
Metal-organic polymer enables efficient organic photoelectrochemical transistor biosensing
Biosens Bioelectron. 2024 Apr 27;257:116346. doi: 10.1016/j.bios.2024.116346. Online ahead of print.ABSTRACTThe field of organic photoelectrochemical transistor (OPECT) is newly emerged, with increasing efforts attempting to utilize its properties in biological sensing. Advanced materials with new physicochemical properties have proven important to this end. Herein, we report a metal-organic polymers-gated OPECT biosensing exemplified by CuⅠ-arylacetylide polymers (CuAs)-modulated poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) channel. Both the photoelectrochemical properties and gating capability o...
Source: Biosensors and Bioelectronics - April 30, 2024 Category: Biotechnology Authors: Cheng Yuan Qiqi Wu Ke-Xin Xu Xing-Shi Liu Hao Lou Yi-Tong Xu Zheng Li Yuanyuan Meng Tan Li Rui Ban Guangxu Chen Wei-Wei Zhao Source Type: research
Enhanced CRISPR/Cas12a-based quantitative detection of nucleic acids using double emulsion droplets
Biosens Bioelectron. 2024 Apr 26;257:116339. doi: 10.1016/j.bios.2024.116339. Online ahead of print.ABSTRACTPairing droplet microfluidics and CRISPR/Cas12a techniques creates a powerful solution for the detection and quantification of nucleic acids at the single-molecule level, due to its specificity, sensitivity, and simplicity. However, traditional water-in-oil (W/O) single emulsion (SE) droplets often present stability issues, affecting the accuracy and reproducibility of assay results. As an alternative, water-in-oil-in-water (W/O/W) double emulsion (DE) droplets offer superior stability and uniformity for droplet digi...
Source: Biosensors and Bioelectronics - April 30, 2024 Category: Biotechnology Authors: Yang Zhang Hangrui Liu Yuta Nakagawa Yuzuki Nagasaka Tianben Ding Shi-Yang Tang Yaxiaer Yalikun Keisuke Goda Ming Li Source Type: research
Development of a CRISPR/Cas12a-mediated aptasensor for Mpox virus antigen detection
Biosens Bioelectron. 2024 Apr 24;257:116313. doi: 10.1016/j.bios.2024.116313. Online ahead of print.ABSTRACTThe emergence and rapid spread of Mpox (formerly monkeypox) have caused significant societal challenges. Adequate and appropriate diagnostics procedures are an urgent necessity. Herein, we discover a pair of aptamers through the systematic evolution of ligands by exponential enrichment (SELEX) that exhibit high affinity and bind to different sites towards the A29 protein of the Mpox virus. Subsequently, we propose a facile, sensitive, convenient CRISPR/Cas12a-mediated aptasensor for detecting the A29 antigen. The pro...
Source: Biosensors and Bioelectronics - April 30, 2024 Category: Biotechnology Authors: Cong Han Qirui Liu Xuantong Luo Jian Zhao Zheng Zhang Jiaxuan He Feng Ge Wei Ding Zhaofeng Luo Chao Jia Liyun Zhang Source Type: research
Metal-organic polymer enables efficient organic photoelectrochemical transistor biosensing
Biosens Bioelectron. 2024 Apr 27;257:116346. doi: 10.1016/j.bios.2024.116346. Online ahead of print.ABSTRACTThe field of organic photoelectrochemical transistor (OPECT) is newly emerged, with increasing efforts attempting to utilize its properties in biological sensing. Advanced materials with new physicochemical properties have proven important to this end. Herein, we report a metal-organic polymers-gated OPECT biosensing exemplified by CuⅠ-arylacetylide polymers (CuAs)-modulated poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) channel. Both the photoelectrochemical properties and gating capability o...
Source: Biosensors and Bioelectronics - April 30, 2024 Category: Biotechnology Authors: Cheng Yuan Qiqi Wu Ke-Xin Xu Xing-Shi Liu Hao Lou Yi-Tong Xu Zheng Li Yuanyuan Meng Tan Li Rui Ban Guangxu Chen Wei-Wei Zhao Source Type: research
Enhanced CRISPR/Cas12a-based quantitative detection of nucleic acids using double emulsion droplets
Biosens Bioelectron. 2024 Apr 26;257:116339. doi: 10.1016/j.bios.2024.116339. Online ahead of print.ABSTRACTPairing droplet microfluidics and CRISPR/Cas12a techniques creates a powerful solution for the detection and quantification of nucleic acids at the single-molecule level, due to its specificity, sensitivity, and simplicity. However, traditional water-in-oil (W/O) single emulsion (SE) droplets often present stability issues, affecting the accuracy and reproducibility of assay results. As an alternative, water-in-oil-in-water (W/O/W) double emulsion (DE) droplets offer superior stability and uniformity for droplet digi...
Source: Biosensors and Bioelectronics - April 30, 2024 Category: Biotechnology Authors: Yang Zhang Hangrui Liu Yuta Nakagawa Yuzuki Nagasaka Tianben Ding Shi-Yang Tang Yaxiaer Yalikun Keisuke Goda Ming Li Source Type: research
Magnetically modified bacteriophage-triggered ATP release activated EXPAR-CRISPR/Cas14a system for visual detection of Burkholderia pseudomallei
Biosens Bioelectron. 2024 Apr 25;257:116334. doi: 10.1016/j.bios.2024.116334. Online ahead of print.ABSTRACTBurkholderia pseudomallei, widely distributed in tropical and subtropical ecosystems, is capable of causing the fatal zoonotic disease melioidosis and exhibiting a global trend of dissemination. Rapid and sensitive detection of B. pseudomallei is essential for environmental monitoring as well as infection control. Here, we developed an innovative biosensor for quantitatively detecting B. pseudomallei relies on ATP released triggered by bacteriophage-induced bacteria lysis. The lytic bacteriophage vB_BpP_HN01, with hi...
Source: Biosensors and Bioelectronics - April 28, 2024 Category: Biotechnology Authors: Juan Yao Zhang Zhang Hua Pei Ting Zhang Yuping Ruan Chenyuan Liu Yongcan Guo Shuo Gu Qianfeng Xia Source Type: research
Optimization and miniaturization of SE-ECL for potential-resolved, multi-color, multi-analyte detection
We present results on the optimization of SE-ECL intensity for different channel lengths and widths, with sum intensities being 5 times larger for 6 cm vs. 2 cm channels and linearly increasing with the width of the channels. Furthermore, we demonstrated for the first time that applying Alternating Current (AC) voltage within the single electrode setup for driving the ECL reactions has a dramatic effect on the emitted light intensity, with square waveforms resulting in higher intensities vs sine waveforms. Additionally, multiplexed multicolor SE-ECL on a 6.5 mm × 3.6 mm CMOS semiconductor image sensor was demonstrated for...
Source: Biosensors and Bioelectronics - April 28, 2024 Category: Biotechnology Authors: Reza Abbasi Sebastian Wachsmann-Hogiu Source Type: research
