Optimization and miniaturization of SE-ECL for potential-resolved, multi-color, multi-analyte detection

We present results on the optimization of SE-ECL intensity for different channel lengths and widths, with sum intensities being 5 times larger for 6 cm vs. 2 cm channels and linearly increasing with the width of the channels. Furthermore, we demonstrated for the first time that applying Alternating Current (AC) voltage within the single electrode setup for driving the ECL reactions has a dramatic effect on the emitted light intensity, with square waveforms resulting in higher intensities vs sine waveforms. Additionally, multiplexed multicolor SE-ECL on a 6.5 mm × 3.6 mm CMOS semiconductor image sensor was demonstrated for the first time, with the ability to simultaneously distinguish four different colors, leading to the ability to measure multiple analytes.PMID:38678789 | DOI:10.1016/j.bios.2024.116322

Source: Biosensors and Bioelectronics - April 28, 2024 Category: Biotechnology Authors: Reza Abbasi Sebastian Wachsmann-Hogiu Source Type: research