Aptamer-bivalent-cholesterol-mediated proximity entropy-driven exosomal protein reporter for tumor diagnosis
Biosens Bioelectron. 2024 Feb 6;251:116104. doi: 10.1016/j.bios.2024.116104. Online ahead of print.ABSTRACTExosomal proteins from the parental cells are considered to be promising biomarker sets for precise tumor diagnostics and monitoring. However, the accurate quantitative analysis of low-abundance exosomal proteins remains challenging due to the heterogeneity of clinical samples. Here, we standardized the exosomal concentration with a fluorogenic membrane probe and developed an aptamer-bivalent-cholesterol-mediated Proximity Entropy-driven Exosomal Protein Reporter (PEEPR). The proposed PEEPR enables the in-situ analysi...
Source: Biosensors and Bioelectronics - February 18, 2024 Category: Biotechnology Authors: Zhichao Fan Jie Zhou Qiuxia Shu Yan Dong Yingxue Li Tingrui Zhang Gang Bai Hua Yu Fanghao Lu Jianjun Li Xiang Zhao Source Type: research
Molecularly imprinted composite-based biosensor for the determination of SARS-CoV-2 nucleocapsid protein
This article aims to present a comparative study of three polypyrrole-based molecularly imprinted polymer (MIP) systems for the detection of the recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (rN). The rN is known for its relatively low propensity to mutate compared to other SARS-CoV-2 antigens. The aforementioned systems include screen-printed carbon electrodes (SPCE) modified with gold nanostructures (MIP1), platinum nanostructures (MIP2), and the unmodified SPCE (MIP3), which was used for control. Pulsed amperometric detection (PAD) was employed as the detection technique, ...
Source: Biosensors and Bioelectronics - February 18, 2024 Category: Biotechnology Authors: Maryia Drobysh Vilma Ratautaite Ernestas Brazys Almira Ramanaviciene Arunas Ramanavicius Source Type: research
Aptamer-bivalent-cholesterol-mediated proximity entropy-driven exosomal protein reporter for tumor diagnosis
Biosens Bioelectron. 2024 Feb 6;251:116104. doi: 10.1016/j.bios.2024.116104. Online ahead of print.ABSTRACTExosomal proteins from the parental cells are considered to be promising biomarker sets for precise tumor diagnostics and monitoring. However, the accurate quantitative analysis of low-abundance exosomal proteins remains challenging due to the heterogeneity of clinical samples. Here, we standardized the exosomal concentration with a fluorogenic membrane probe and developed an aptamer-bivalent-cholesterol-mediated Proximity Entropy-driven Exosomal Protein Reporter (PEEPR). The proposed PEEPR enables the in-situ analysi...
Source: Biosensors and Bioelectronics - February 18, 2024 Category: Biotechnology Authors: Zhichao Fan Jie Zhou Qiuxia Shu Yan Dong Yingxue Li Tingrui Zhang Gang Bai Hua Yu Fanghao Lu Jianjun Li Xiang Zhao Source Type: research
Passive trapping of biomolecules in hotspots with all-dielectric terahertz metamaterials
Biosens Bioelectron. 2024 Feb 12;251:116126. doi: 10.1016/j.bios.2024.116126. Online ahead of print.ABSTRACTElectromagnetic metamaterials feature the capability of squeezing photons into hotspot regions of high intensity near-field enhancement for strong light-matter interaction, underpinning the next generation of emerging biosensors. However, randomly dispersed biomolecules around the hotspots lead to weak interactions. Here, we demonstrate an all-silicon dielectric terahertz metamaterial sensor design capable of passively trapping biomoleculars into the resonant cavities confined with powerful electric field. Specifical...
Source: Biosensors and Bioelectronics - February 17, 2024 Category: Biotechnology Authors: Tingling Lin Yi Huang Shuncong Zhong Tingting Shi Fuwei Sun Yujie Zhong Qiuming Zeng Qiukun Zhang Daxiang Cui Source Type: research
A 3D-printed hollow microneedle-based electrochemical sensing device for in situ plant health monitoring
Biosens Bioelectron. 2024 Feb 13;251:116131. doi: 10.1016/j.bios.2024.116131. Online ahead of print.ABSTRACTPlant health monitoring is devised as a new concept to elucidate in situ physiological processes. The need for increased food production to nourish the growing global population is inconsistent with the dramatic impact of climate change, which hinders crop health and exacerbates plant stress. In this context, wearable sensors play a crucial role in assessing plant stress. Herein, we present a low-cost 3D-printed hollow microneedle array (HMA) patch as a sampling device coupled with biosensors based on screen-printing...
Source: Biosensors and Bioelectronics - February 17, 2024 Category: Biotechnology Authors: Marc Parrilla Amadeo Sena-Torralba Annemarijn Steijlen Sergi Morais Ángel Maquieira Karolien De Wael Source Type: research
Surface-functionalized SERS platform for deep learning-assisted diagnosis of Alzheimer's disease
Biosens Bioelectron. 2024 Feb 12;251:116128. doi: 10.1016/j.bios.2024.116128. Online ahead of print.ABSTRACTEarly diagnosis of Alzheimer's disease is crucial to stall the deterioration of brain function, but conventional diagnostic methods require complicated analytical procedures or inflict acute pain on the patient. Then, label-free Surface-enhanced Raman spectroscopy (SERS) analysis of blood-based biomarkers is a convenient alternative to rapidly obtain spectral information from biofluids. However, despite the rapid acquisition of spectral information from biofluids, it is challenging to distinguish spectral features of...
Source: Biosensors and Bioelectronics - February 17, 2024 Category: Biotechnology Authors: Minjoon Kim Sejoon Huh Hyung Joon Park Seunghee H Cho Min-Young Lee Sungho Jo Yeon Sik Jung Source Type: research
Ammonia detection: A pathway towards potential point-of-care diagnostics
Biosens Bioelectron. 2024 Feb 2;251:116100. doi: 10.1016/j.bios.2024.116100. Online ahead of print.ABSTRACTInvasive methods such as blood collection and biopsy are commonly used for testing liver and kidney function, which are painful, time-consuming, require trained personnel, and may not be easily accessible to people for their routine checkup. Early diagnosis of liver and kidney diseases can prevent severe symptoms and ensure better management of these patients. Emerging approaches such as breath and sweat analysis have shown potential as non-invasive methods for disease diagnosis. Among the many markers, ammonia is oft...
Source: Biosensors and Bioelectronics - February 16, 2024 Category: Biotechnology Authors: Ronil J Rath Jack O Herrington Muhammad Adeel Firat G üder Fariba Dehghani Syamak Farajikhah Source Type: research
A cardiomyocyte-based biosensing platform for dynamic and quantitative investigation of excessive autophagy
Biosens Bioelectron. 2024 Feb 13;251:116113. doi: 10.1016/j.bios.2024.116113. Online ahead of print.ABSTRACTAutophagy is an important physiological phenomenon in eukaryotes that helps maintain the cellular homeostasis. Autophagy is involved in the development of various cardiovascular diseases, affecting the maintenance of cardiac function and disease prognosis. Physiological levels of autophagy serve as a defense mechanism for cardiomyocytes against environmental stimuli, but an overabundance of autophagy may contribute to the development of cardiovascular diseases. However, conventional biological methods are difficult t...
Source: Biosensors and Bioelectronics - February 16, 2024 Category: Biotechnology Authors: Cheng-Wen He Chunlian Qin Yi Zhang Yan Zhang Kaiqiang Li Yuqun Cai Wei Zhang Ning Hu Zhen Wang Source Type: research
A MoS < sub > 2 < /sub > nanosheet-based CRISPR/Cas12a biosensor for efficient miRNA quantification for acute myocardial infarction
We describe herein the first example of MoS2 nanosheet-based signal amplified fluorescence sensor for effective detection of AMI-related miRNA.PMID:38364329 | DOI:10.1016/j.bios.2024.116129 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - February 16, 2024 Category: Biotechnology Authors: Peng Li Yu Ye Yang Li Zhuohao Xie Lei Ye Jiahao Huang Source Type: research
Ammonia detection: A pathway towards potential point-of-care diagnostics
Biosens Bioelectron. 2024 Feb 2;251:116100. doi: 10.1016/j.bios.2024.116100. Online ahead of print.ABSTRACTInvasive methods such as blood collection and biopsy are commonly used for testing liver and kidney function, which are painful, time-consuming, require trained personnel, and may not be easily accessible to people for their routine checkup. Early diagnosis of liver and kidney diseases can prevent severe symptoms and ensure better management of these patients. Emerging approaches such as breath and sweat analysis have shown potential as non-invasive methods for disease diagnosis. Among the many markers, ammonia is oft...
Source: Biosensors and Bioelectronics - February 16, 2024 Category: Biotechnology Authors: Ronil J Rath Jack O Herrington Muhammad Adeel Firat G üder Fariba Dehghani Syamak Farajikhah Source Type: research
A cardiomyocyte-based biosensing platform for dynamic and quantitative investigation of excessive autophagy
Biosens Bioelectron. 2024 Feb 13;251:116113. doi: 10.1016/j.bios.2024.116113. Online ahead of print.ABSTRACTAutophagy is an important physiological phenomenon in eukaryotes that helps maintain the cellular homeostasis. Autophagy is involved in the development of various cardiovascular diseases, affecting the maintenance of cardiac function and disease prognosis. Physiological levels of autophagy serve as a defense mechanism for cardiomyocytes against environmental stimuli, but an overabundance of autophagy may contribute to the development of cardiovascular diseases. However, conventional biological methods are difficult t...
Source: Biosensors and Bioelectronics - February 16, 2024 Category: Biotechnology Authors: Cheng-Wen He Chunlian Qin Yi Zhang Yan Zhang Kaiqiang Li Yuqun Cai Wei Zhang Ning Hu Zhen Wang Source Type: research
A MoS < sub > 2 < /sub > nanosheet-based CRISPR/Cas12a biosensor for efficient miRNA quantification for acute myocardial infarction
We describe herein the first example of MoS2 nanosheet-based signal amplified fluorescence sensor for effective detection of AMI-related miRNA.PMID:38364329 | DOI:10.1016/j.bios.2024.116129 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - February 16, 2024 Category: Biotechnology Authors: Peng Li Yu Ye Yang Li Zhuohao Xie Lei Ye Jiahao Huang Source Type: research
Portable droplet-based real-time monitoring of pancreatic α-amylase in postoperative patients
We report a bedside portable droplet-based millifluidic device enabling real-time sensing of drain α-amylase activity for postoperative monitoring of patients undergoing pancreatic surgery. Here, a tiny amount of drain liquid of patient samples is continuously collected and co-encapsulated with a starch reagent in nanoliter-sized droplets to track the fluorescence intensity released upon reaction with α-amylase. Comparing the α-amylase levels of 32 patients, 97 % of the results of the droplet-based millifluidic system matched the clinical data. Our method reduces the α-amylase assay duration to approximately 3 min with...
Source: Biosensors and Bioelectronics - February 15, 2024 Category: Biotechnology Authors: Xinne Zhao Fiona R Kolbinger Marius Distler J ürgen Weitz Denys Makarov Michael Bachmann Larysa Baraban Source Type: research
Combining plasmonic and electrochemical biosensing methods
Biosens Bioelectron. 2024 Feb 10;251:116098. doi: 10.1016/j.bios.2024.116098. Online ahead of print.ABSTRACTThe idea of combining electrochemical (EC) and plasmonic biosensor methods was introduced almost thirty years ago and the potential of electrochemical-plasmonic (EC-P) biosensors has been highlighted ever since. Despite that, the use of EC-P biosensors in analytics has been rather limited so far and the search for unique applications of the EC-P method continues. In this paper, we review the advances in the field of EC-P biosensors and discuss the features and benefits they can provide. In addition, we identify the m...
Source: Biosensors and Bioelectronics - February 15, 2024 Category: Biotechnology Authors: Erika Hemmerov á Ji ří Homola Source Type: research
Probe-assisted detection of Fe < sup > 3+ < /sup > ions in a multi-functionalized nanopore
In this study, we report an innovative Fe3+ detection strategy by using both a ligand probe and an engineered nanopore with two binding sites. In our design, one binding site of the nanopore has a strong interaction with the ligand probe, while the other is more selective toward interfering species. Based on the difference in the number of ligand DTPMPA events in the absence and presence of ferric ions, micromolar concentrations of Fe3+ could be detected within minutes. Our method is selective: micromolar concentrations of Mg2+, Ca2+, Cd2+, Zn2+, Ni2+, Co2+, Mn2+, and Cu2+ would not interfere with the detection of ferric i...
Source: Biosensors and Bioelectronics - February 15, 2024 Category: Biotechnology Authors: Pearl Arora Haiyan Zheng Sathishkumar Munusamy Rana Jahani Liang Wang Xiyun Guan Source Type: research
