Improvement of acetyl-CoA supply and glucose utilization increases l-leucine production in Corynebacterium glutamicum
CONCLUSIONS AND IMPLICATIONS: These results represent a significant improvement in the l-leucine titer of C. glutamicum, indicating that the process possesses highly potential for industrial application. These strategies can be also expanded to enable the production of other value-added biochemicals derived from the intermediates of central carbon metabolism. This article is protected by copyright. All rights reserved.PMID:34870372 | DOI:10.1002/biot.202100349 (Source: Biotechnology Journal)
Source: Biotechnology Journal - December 6, 2021 Category: Biotechnology Authors: Yingyu Wang Jianzhong Xu Zhengyu Jin Xiaole Xia Weiguo Zhang Source Type: research

Deletion analysis of Pichia pastoris alcohol dehydrogenase 2 (ADH2) promoter and development of synthetic promoters
This article is protected by copyright. All rights reserved.PMID:34870891 | DOI:10.1002/biot.202100332 (Source: Biotechnology Journal)
Source: Biotechnology Journal - December 6, 2021 Category: Biotechnology Authors: Fidan Erden-Karao ğlan Mert Karao ğlan G ürkan Yılmaz Semiramis Y ılmaz Mehmet İnan Source Type: research

Rational hinge engineering of carboxylic acid reductase from Mycobacterium smegmatis enhances its catalytic efficiency in biocatalysis
CONCLUSION: Altogether, our two-step strategy used here provided useful references for the engineering of CARs and other similar multiple-domain enzymes. This article is protected by copyright. All rights reserved.PMID:34862729 | DOI:10.1002/biot.202100441 (Source: Biotechnology Journal)
Source: Biotechnology Journal - December 4, 2021 Category: Biotechnology Authors: Liuzhu Wang Yangyang Sun Shiqing Diao Shuiqin Jiang Hualei Wang Dongzhi Wei Source Type: research

Rational hinge engineering of carboxylic acid reductase from Mycobacterium smegmatis enhances its catalytic efficiency in biocatalysis
CONCLUSION: Altogether, our two-step strategy used here provided useful references for the engineering of CARs and other similar multiple-domain enzymes. This article is protected by copyright. All rights reserved.PMID:34862729 | DOI:10.1002/biot.202100441 (Source: Biotechnology Journal)
Source: Biotechnology Journal - December 4, 2021 Category: Biotechnology Authors: Liuzhu Wang Yangyang Sun Shiqing Diao Shuiqin Jiang Hualei Wang Dongzhi Wei Source Type: research

Single-strand annealing: Molecular mechanisms and potential applications in CRISPR-Cas-based precision genome editing
CONCLUSION: The SSA mechanism involved in DNA DSB repair appears to be activated by a complex signaling cascade starting with broken end sensing and 5'-3' resection to reveal homologous repeats on the 3' ssDNA overhangs that flank the DSB. Annealing the repeats would help to amend the discontinuous ends and restore the intact genome, resulting in the missing one repeat and the intervening sequence between the repeats. We proposed a model for CRISPR-Cas-based precision insertion or replacement of DNA fragments to take advantage of the characteristics. The proposed model can add a tool to extend the choice for precision gene...
Source: Biotechnology Journal - November 30, 2021 Category: Biotechnology Authors: Tien Van Vu Swati Das Cam Chau Nguyen Jihae Kim Jae-Yean Kim Source Type: research

Single-strand annealing: Molecular mechanisms and potential applications in CRISPR-Cas-based precision genome editing
CONCLUSION: The SSA mechanism involved in DNA DSB repair appears to be activated by a complex signaling cascade starting with broken end sensing and 5'-3' resection to reveal homologous repeats on the 3' ssDNA overhangs that flank the DSB. Annealing the repeats would help to amend the discontinuous ends and restore the intact genome, resulting in the missing one repeat and the intervening sequence between the repeats. We proposed a model for CRISPR-Cas-based precision insertion or replacement of DNA fragments to take advantage of the characteristics. The proposed model can add a tool to extend the choice for precision gene...
Source: Biotechnology Journal - November 30, 2021 Category: Biotechnology Authors: Tien Van Vu Swati Das Cam Chau Nguyen Jihae Kim Jae-Yean Kim Source Type: research

Single-strand annealing: Molecular mechanisms and potential applications in CRISPR-Cas-based precision genome editing
CONCLUSION: The SSA mechanism involved in DNA DSB repair appears to be activated by a complex signaling cascade starting with broken end sensing and 5'-3' resection to reveal homologous repeats on the 3' ssDNA overhangs that flank the DSB. Annealing the repeats would help to amend the discontinuous ends and restore the intact genome, resulting in the missing one repeat and the intervening sequence between the repeats. We proposed a model for CRISPR-Cas-based precision insertion or replacement of DNA fragments to take advantage of the characteristics. The proposed model can add a tool to extend the choice for precision gene...
Source: Biotechnology Journal - November 30, 2021 Category: Biotechnology Authors: Tien Van Vu Swati Das Cam Chau Nguyen Jihae Kim Jae-Yean Kim Source Type: research

Single-strand annealing: Molecular mechanisms and potential applications in CRISPR-Cas-based precision genome editing
CONCLUSION: The SSA mechanism involved in DNA DSB repair appears to be activated by a complex signaling cascade starting with broken end sensing and 5'-3' resection to reveal homologous repeats on the 3' ssDNA overhangs that flank the DSB. Annealing the repeats would help to amend the discontinuous ends and restore the intact genome, resulting in the missing one repeat and the intervening sequence between the repeats. We proposed a model for CRISPR-Cas-based precision insertion or replacement of DNA fragments to take advantage of the characteristics. The proposed model can add a tool to extend the choice for precision gene...
Source: Biotechnology Journal - November 30, 2021 Category: Biotechnology Authors: Tien Van Vu Swati Das Cam Chau Nguyen Jihae Kim Jae-Yean Kim Source Type: research

CRISPRa/i with adaptive single guide assisted regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production
This article is protected by copyright. All rights reserved.PMID:34800080 | DOI:10.1002/biot.202100514 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Jia-Yi Lin Way-Rong Lin I-Son Ng Source Type: research

Key Criteria for Engineering Mycotoxin Binding Aptamers via Computational Simulations: Aflatoxin B1 as a Case Study
This article is protected by copyright. All rights reserved.PMID:34800084 | DOI:10.1002/biot.202100280 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Maryam Mousivand Kowsar Bagherzadeh Laura Anfossi Mohammad Javan-Nikkhah Source Type: research

CRISPRa/i with adaptive single guide assisted regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production
This article is protected by copyright. All rights reserved.PMID:34800080 | DOI:10.1002/biot.202100514 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Jia-Yi Lin Way-Rong Lin I-Son Ng Source Type: research

Key Criteria for Engineering Mycotoxin Binding Aptamers via Computational Simulations: Aflatoxin B1 as a Case Study
This article is protected by copyright. All rights reserved.PMID:34800084 | DOI:10.1002/biot.202100280 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Maryam Mousivand Kowsar Bagherzadeh Laura Anfossi Mohammad Javan-Nikkhah Source Type: research

CRISPRa/i with adaptive single guide assisted regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production
This article is protected by copyright. All rights reserved.PMID:34800080 | DOI:10.1002/biot.202100514 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Jia-Yi Lin Way-Rong Lin I-Son Ng Source Type: research

Key Criteria for Engineering Mycotoxin Binding Aptamers via Computational Simulations: Aflatoxin B1 as a Case Study
This article is protected by copyright. All rights reserved.PMID:34800084 | DOI:10.1002/biot.202100280 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Maryam Mousivand Kowsar Bagherzadeh Laura Anfossi Mohammad Javan-Nikkhah Source Type: research

CRISPRa/i with adaptive single guide assisted regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production
This article is protected by copyright. All rights reserved.PMID:34800080 | DOI:10.1002/biot.202100514 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Jia-Yi Lin Way-Rong Lin I-Son Ng Source Type: research

Key Criteria for Engineering Mycotoxin Binding Aptamers via Computational Simulations: Aflatoxin B1 as a Case Study
This article is protected by copyright. All rights reserved.PMID:34800084 | DOI:10.1002/biot.202100280 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Maryam Mousivand Kowsar Bagherzadeh Laura Anfossi Mohammad Javan-Nikkhah Source Type: research

CRISPRa/i with adaptive single guide assisted regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production
This article is protected by copyright. All rights reserved.PMID:34800080 | DOI:10.1002/biot.202100514 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Jia-Yi Lin Way-Rong Lin I-Son Ng Source Type: research

Key Criteria for Engineering Mycotoxin Binding Aptamers via Computational Simulations: Aflatoxin B1 as a Case Study
This article is protected by copyright. All rights reserved.PMID:34800084 | DOI:10.1002/biot.202100280 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Maryam Mousivand Kowsar Bagherzadeh Laura Anfossi Mohammad Javan-Nikkhah Source Type: research

CRISPRa/i with adaptive single guide assisted regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production
This article is protected by copyright. All rights reserved.PMID:34800080 | DOI:10.1002/biot.202100514 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Jia-Yi Lin Way-Rong Lin I-Son Ng Source Type: research

Key Criteria for Engineering Mycotoxin Binding Aptamers via Computational Simulations: Aflatoxin B1 as a Case Study
This article is protected by copyright. All rights reserved.PMID:34800084 | DOI:10.1002/biot.202100280 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Maryam Mousivand Kowsar Bagherzadeh Laura Anfossi Mohammad Javan-Nikkhah Source Type: research

CRISPRa/i with adaptive single guide assisted regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production
This article is protected by copyright. All rights reserved.PMID:34800080 | DOI:10.1002/biot.202100514 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Jia-Yi Lin Way-Rong Lin I-Son Ng Source Type: research

Key Criteria for Engineering Mycotoxin Binding Aptamers via Computational Simulations: Aflatoxin B1 as a Case Study
This article is protected by copyright. All rights reserved.PMID:34800084 | DOI:10.1002/biot.202100280 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Maryam Mousivand Kowsar Bagherzadeh Laura Anfossi Mohammad Javan-Nikkhah Source Type: research

CRISPRa/i with adaptive single guide assisted regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production
This article is protected by copyright. All rights reserved.PMID:34800080 | DOI:10.1002/biot.202100514 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Jia-Yi Lin Way-Rong Lin I-Son Ng Source Type: research

Key Criteria for Engineering Mycotoxin Binding Aptamers via Computational Simulations: Aflatoxin B1 as a Case Study
This article is protected by copyright. All rights reserved.PMID:34800084 | DOI:10.1002/biot.202100280 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 20, 2021 Category: Biotechnology Authors: Maryam Mousivand Kowsar Bagherzadeh Laura Anfossi Mohammad Javan-Nikkhah Source Type: research

Ultrasound assisted in-situ separation of sophorolipids in multi-phase fermentation system to achieve efficient production by Candida bombicola
CONCLUSIONS: This study provides a new horizon for optimization of the SLs fermentation process. This article is protected by copyright. All rights reserved.PMID:34792852 | DOI:10.1002/biot.202100478 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 18, 2021 Category: Biotechnology Authors: Yang Chen Xu Tang Ya Li Chang Liu Yingping Zhuang Xiwei Tian Ju Chu Source Type: research

Ultrasound assisted in-situ separation of sophorolipids in multi-phase fermentation system to achieve efficient production by Candida bombicola
CONCLUSIONS: This study provides a new horizon for optimization of the SLs fermentation process. This article is protected by copyright. All rights reserved.PMID:34792852 | DOI:10.1002/biot.202100478 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 18, 2021 Category: Biotechnology Authors: Yang Chen Xu Tang Ya Li Chang Liu Yingping Zhuang Xiwei Tian Ju Chu Source Type: research

Ultrasound assisted in-situ separation of sophorolipids in multi-phase fermentation system to achieve efficient production by Candida bombicola
CONCLUSIONS: This study provides a new horizon for optimization of the SLs fermentation process. This article is protected by copyright. All rights reserved.PMID:34792852 | DOI:10.1002/biot.202100478 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 18, 2021 Category: Biotechnology Authors: Yang Chen Xu Tang Ya Li Chang Liu Yingping Zhuang Xiwei Tian Ju Chu Source Type: research

Massively parallel DNA target capture using long adapter single stranded oligonucleotide (LASSO) probes assembled through a novel DNA recombinase mediated methodology
This article is protected by copyright. All rights reserved.PMID:34775678 | DOI:10.1002/biot.202100240 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 14, 2021 Category: Biotechnology Authors: Lorenzo Tosi Lamia Chaikban Benjamin H Larman Jeffrey Rosenfield Biju Parekkadan Source Type: research

Massively parallel DNA target capture using long adapter single stranded oligonucleotide (LASSO) probes assembled through a novel DNA recombinase mediated methodology
This article is protected by copyright. All rights reserved.PMID:34775678 | DOI:10.1002/biot.202100240 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 14, 2021 Category: Biotechnology Authors: Lorenzo Tosi Lamia Chaikban Benjamin H Larman Jeffrey Rosenfield Biju Parekkadan Source Type: research

Massively parallel DNA target capture using long adapter single stranded oligonucleotide (LASSO) probes assembled through a novel DNA recombinase mediated methodology
This article is protected by copyright. All rights reserved.PMID:34775678 | DOI:10.1002/biot.202100240 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 14, 2021 Category: Biotechnology Authors: Lorenzo Tosi Lamia Chaikban Benjamin H Larman Jeffrey Rosenfield Biju Parekkadan Source Type: research

T-cell surface generation of dual bivalent, bispecific T-cell engaging, RNA duplex cross-linked antibodies (dbBiTERs) for re-directed tumor cell lysis
CONCLUSION: The facile generation of dbBiTERs with specific cytolytic activity from intact antibodies and their generation on-cell offers a new avenue for antigen specific T-cell therapy. This article is protected by copyright. All rights reserved.PMID:34773368 | DOI:10.1002/biot.202100389 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 13, 2021 Category: Biotechnology Authors: Maciej Kujawski Lin Li Harry Li Paul J Yazaki Piotr Swiderski John E Shively Source Type: research

T-cell surface generation of dual bivalent, bispecific T-cell engaging, RNA duplex cross-linked antibodies (dbBiTERs) for re-directed tumor cell lysis
CONCLUSION: The facile generation of dbBiTERs with specific cytolytic activity from intact antibodies and their generation on-cell offers a new avenue for antigen specific T-cell therapy. This article is protected by copyright. All rights reserved.PMID:34773368 | DOI:10.1002/biot.202100389 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 13, 2021 Category: Biotechnology Authors: Maciej Kujawski Lin Li Harry Li Paul J Yazaki Piotr Swiderski John E Shively Source Type: research

Mechanistic discoveries and simulation-guided assay optimization of portable hormone biosensors with cell-free protein synthesis
This article is protected by copyright. All rights reserved.PMID:34761537 | DOI:10.1002/biot.202100152 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 11, 2021 Category: Biotechnology Authors: J Porter Hunt Jackelyn Galiardi Tyler J Free Seung Ook Yang Daniel Poole Emily L Zhao Joshua L Andersen David W Wood Bradley C Bundy Source Type: research

Mechanistic discoveries and simulation-guided assay optimization of portable hormone biosensors with cell-free protein synthesis
This article is protected by copyright. All rights reserved.PMID:34761537 | DOI:10.1002/biot.202100152 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 11, 2021 Category: Biotechnology Authors: J Porter Hunt Jackelyn Galiardi Tyler J Free Seung Ook Yang Daniel Poole Emily L Zhao Joshua L Andersen David W Wood Bradley C Bundy Source Type: research

Mechanistic discoveries and simulation-guided assay optimization of portable hormone biosensors with cell-free protein synthesis
This article is protected by copyright. All rights reserved.PMID:34761537 | DOI:10.1002/biot.202100152 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 11, 2021 Category: Biotechnology Authors: J Porter Hunt Jackelyn Galiardi Tyler J Free Seung Ook Yang Daniel Poole Emily L Zhao Joshua L Andersen David W Wood Bradley C Bundy Source Type: research

Green and ecofriendly biosynthesis of selenium nanoparticles using Urtica dioica (Stinging nettle) leaf extract: Antimicrobial and anticancer activity
CONCLUSIONS AND IMPLICATIONS: For the first time, aqueous stinging nettle leaf extract was utilized to biosynthesise SeNPs in a green method. SeNPs have outstanding antimicrobial-activity against pathogenic bacterial and fungal strains. Moreover, SeNPs have promising anticancer activity against HepG2 cancerous cell line without cytotoxicity on Vero normal cell line. Finally, the biosynthesized SeNPs via aqueous extract of stinging nettle leaf exhibited potential antibacterial, antifungal and anticancer action, making them useful in the medical field. This article is protected by copyright. All rights reserved.PMID:34747563...
Source: Biotechnology Journal - November 8, 2021 Category: Biotechnology Authors: Amr H Hashem Salem S Salem Source Type: research

Epitope Screening Using Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS): An Accelerated Workflow for Evaluation of Lead Monoclonal Antibodies
CONCLUSION: An accelerated HDX-MS epitope screening workflow was developed. The 'screening' workflow successfully characterized five (out of six attempted) novel epitopes on the PRN antigen; information that can be used to support vaccine antigenicity assays. This article is protected by copyright. All rights reserved.PMID:34747565 | DOI:10.1002/biot.202100358 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 8, 2021 Category: Biotechnology Authors: Shaolong Zhu Peter Liuni Tricia Chen Camille Houy Derek J Wilson D Andrew James Source Type: research

Green and ecofriendly biosynthesis of selenium nanoparticles using Urtica dioica (Stinging nettle) leaf extract: Antimicrobial and anticancer activity
CONCLUSIONS AND IMPLICATIONS: For the first time, aqueous stinging nettle leaf extract was utilized to biosynthesise SeNPs in a green method. SeNPs have outstanding antimicrobial-activity against pathogenic bacterial and fungal strains. Moreover, SeNPs have promising anticancer activity against HepG2 cancerous cell line without cytotoxicity on Vero normal cell line. Finally, the biosynthesized SeNPs via aqueous extract of stinging nettle leaf exhibited potential antibacterial, antifungal and anticancer action, making them useful in the medical field. This article is protected by copyright. All rights reserved.PMID:34747563...
Source: Biotechnology Journal - November 8, 2021 Category: Biotechnology Authors: Amr H Hashem Salem S Salem Source Type: research

Epitope Screening Using Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS): An Accelerated Workflow for Evaluation of Lead Monoclonal Antibodies
CONCLUSION: An accelerated HDX-MS epitope screening workflow was developed. The 'screening' workflow successfully characterized five (out of six attempted) novel epitopes on the PRN antigen; information that can be used to support vaccine antigenicity assays. This article is protected by copyright. All rights reserved.PMID:34747565 | DOI:10.1002/biot.202100358 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 8, 2021 Category: Biotechnology Authors: Shaolong Zhu Peter Liuni Tricia Chen Camille Houy Derek J Wilson D Andrew James Source Type: research

Green and ecofriendly biosynthesis of selenium nanoparticles using Urtica dioica (Stinging nettle) leaf extract: Antimicrobial and anticancer activity
CONCLUSIONS AND IMPLICATIONS: For the first time, aqueous stinging nettle leaf extract was utilized to biosynthesise SeNPs in a green method. SeNPs have outstanding antimicrobial-activity against pathogenic bacterial and fungal strains. Moreover, SeNPs have promising anticancer activity against HepG2 cancerous cell line without cytotoxicity on Vero normal cell line. Finally, the biosynthesized SeNPs via aqueous extract of stinging nettle leaf exhibited potential antibacterial, antifungal and anticancer action, making them useful in the medical field. This article is protected by copyright. All rights reserved.PMID:34747563...
Source: Biotechnology Journal - November 8, 2021 Category: Biotechnology Authors: Amr H Hashem Salem S Salem Source Type: research

Epitope Screening Using Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS): An Accelerated Workflow for Evaluation of Lead Monoclonal Antibodies
CONCLUSION: An accelerated HDX-MS epitope screening workflow was developed. The 'screening' workflow successfully characterized five (out of six attempted) novel epitopes on the PRN antigen; information that can be used to support vaccine antigenicity assays. This article is protected by copyright. All rights reserved.PMID:34747565 | DOI:10.1002/biot.202100358 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 8, 2021 Category: Biotechnology Authors: Shaolong Zhu Peter Liuni Tricia Chen Camille Houy Derek J Wilson D Andrew James Source Type: research

Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads
CONCLUSIONS: The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine. This article is protected by copyright. All rights reserved.PMID:34743417 | DOI:10.1002/biot.202100298 (Source: Biotechnology Journal)
Source: Biotechnology Journal - November 7, 2021 Category: Biotechnology Authors: Sawsan Dacrory Amr Hosny Hashem Samir Kamel Source Type: research

A BioBricks ® toolbox for metabolic engineering of the tetracenomycin pathway
CONCLUSIONS AND IMPLICATIONS: Altogether, this system is compatible with the BioBricks® [RFC 10] cloning standard for the co-expression of multiple gene sets for metabolic engineering of Streptomyces coelicolor M1146::cos16F4iE. This production platform improves access to potent analogs, such as tetracenomycin X, and sets the stage for the production of new tetracenomycins via combinatorial biosynthesis. This article is protected by copyright. All rights reserved.PMID:34719127 | DOI:10.1002/biot.202100371 (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 31, 2021 Category: Biotechnology Authors: Jennifer T Nguyen Kennedy K Riebschleger Katelyn V Brown Nina M Gorgijevska S Eric Nybo Source Type: research

A BioBricks ® toolbox for metabolic engineering of the tetracenomycin pathway
CONCLUSIONS AND IMPLICATIONS: Altogether, this system is compatible with the BioBricks® [RFC 10] cloning standard for the co-expression of multiple gene sets for metabolic engineering of Streptomyces coelicolor M1146::cos16F4iE. This production platform improves access to potent analogs, such as tetracenomycin X, and sets the stage for the production of new tetracenomycins via combinatorial biosynthesis. This article is protected by copyright. All rights reserved.PMID:34719127 | DOI:10.1002/biot.202100371 (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 31, 2021 Category: Biotechnology Authors: Jennifer T Nguyen Kennedy K Riebschleger Katelyn V Brown Nina M Gorgijevska S Eric Nybo Source Type: research

A BioBricks ® toolbox for metabolic engineering of the tetracenomycin pathway
CONCLUSIONS AND IMPLICATIONS: Altogether, this system is compatible with the BioBricks® [RFC 10] cloning standard for the co-expression of multiple gene sets for metabolic engineering of Streptomyces coelicolor M1146::cos16F4iE. This production platform improves access to potent analogs, such as tetracenomycin X, and sets the stage for the production of new tetracenomycins via combinatorial biosynthesis. This article is protected by copyright. All rights reserved.PMID:34719127 | DOI:10.1002/biot.202100371 (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 31, 2021 Category: Biotechnology Authors: Jennifer T Nguyen Kennedy K Riebschleger Katelyn V Brown Nina M Gorgijevska S Eric Nybo Source Type: research

A BioBricks ® toolbox for metabolic engineering of the tetracenomycin pathway
CONCLUSIONS AND IMPLICATIONS: Altogether, this system is compatible with the BioBricks® [RFC 10] cloning standard for the co-expression of multiple gene sets for metabolic engineering of Streptomyces coelicolor M1146::cos16F4iE. This production platform improves access to potent analogs, such as tetracenomycin X, and sets the stage for the production of new tetracenomycins via combinatorial biosynthesis. This article is protected by copyright. All rights reserved.PMID:34719127 | DOI:10.1002/biot.202100371 (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 31, 2021 Category: Biotechnology Authors: Jennifer T Nguyen Kennedy K Riebschleger Katelyn V Brown Nina M Gorgijevska S Eric Nybo Source Type: research

A BioBricks ® toolbox for metabolic engineering of the tetracenomycin pathway
CONCLUSIONS AND IMPLICATIONS: Altogether, this system is compatible with the BioBricks® [RFC 10] cloning standard for the co-expression of multiple gene sets for metabolic engineering of Streptomyces coelicolor M1146::cos16F4iE. This production platform improves access to potent analogs, such as tetracenomycin X, and sets the stage for the production of new tetracenomycins via combinatorial biosynthesis. This article is protected by copyright. All rights reserved.PMID:34719127 | DOI:10.1002/biot.202100371 (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 31, 2021 Category: Biotechnology Authors: Jennifer T Nguyen Kennedy K Riebschleger Katelyn V Brown Nina M Gorgijevska S Eric Nybo Source Type: research

A BioBricks ® toolbox for metabolic engineering of the tetracenomycin pathway
CONCLUSIONS AND IMPLICATIONS: Altogether, this system is compatible with the BioBricks® [RFC 10] cloning standard for the co-expression of multiple gene sets for metabolic engineering of Streptomyces coelicolor M1146::cos16F4iE. This production platform improves access to potent analogs, such as tetracenomycin X, and sets the stage for the production of new tetracenomycins via combinatorial biosynthesis. This article is protected by copyright. All rights reserved.PMID:34719127 | DOI:10.1002/biot.202100371 (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 31, 2021 Category: Biotechnology Authors: Jennifer T Nguyen Kennedy K Riebschleger Katelyn V Brown Nina M Gorgijevska S Eric Nybo Source Type: research

Targeting metabolic driving and minimization of by-products synthesis for high-yield production of D-pantothenate in Escherichia coli
CONCLUSION: This study paved a foundation for the industrial production of DPA. This article is protected by copyright. All rights reserved.PMID:34705325 | DOI:10.1002/biot.202100431 (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 27, 2021 Category: Biotechnology Authors: Bo Li Bo Zhang Pei Wang Xue Cai Ya-Qun Tang Jie-Yi Jin Jin-Xi Liang Zhi-Qiang Liu Yu-Guo Zheng Source Type: research

Establishment of a pig CRISPR/Cas9 knockout library for functional gene screening in pig cells
CONCLUSIONS: Our CRISPR knockout library targeting pig whole genome establishes a promising platform for pig functional genomic analysis. This article is protected by copyright. All rights reserved.PMID:34705337 | DOI:10.1002/biot.202100408 (Source: Biotechnology Journal)
Source: Biotechnology Journal - October 27, 2021 Category: Biotechnology Authors: Chuanzhao Yu Haiwen Zhong Xiaohui Yang Guoling Li Zhenfang Wu Huaqiang Yang Source Type: research