Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production - A State of the art review
CONCLUSION: Optimization strategies, which are being explored in isolation, need to be logically integrated for the successful commercialization of microbial PHAs. This article is protected by copyright. All rights reserved.PMID:34132046 | DOI:10.1002/biot.202100136 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 16, 2021 Category: Biotechnology Authors: Pema Lhamo Shishir Kumar Behera Biswanath Mahanty Source Type: research

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production - A State of the art review
CONCLUSION: Optimization strategies, which are being explored in isolation, need to be logically integrated for the successful commercialization of microbial PHAs. This article is protected by copyright. All rights reserved.PMID:34132046 | DOI:10.1002/biot.202100136 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 16, 2021 Category: Biotechnology Authors: Pema Lhamo Shishir Kumar Behera Biswanath Mahanty Source Type: research

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production - A State of the art review
CONCLUSION: Optimization strategies, which are being explored in isolation, need to be logically integrated for the successful commercialization of microbial PHAs. This article is protected by copyright. All rights reserved.PMID:34132046 | DOI:10.1002/biot.202100136 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 16, 2021 Category: Biotechnology Authors: Pema Lhamo Shishir Kumar Behera Biswanath Mahanty Source Type: research

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production - A State of the art review
CONCLUSION: Optimization strategies, which are being explored in isolation, need to be logically integrated for the successful commercialization of microbial PHAs. This article is protected by copyright. All rights reserved.PMID:34132046 | DOI:10.1002/biot.202100136 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 16, 2021 Category: Biotechnology Authors: Pema Lhamo Shishir Kumar Behera Biswanath Mahanty Source Type: research

Spatial transcriptomics and proteomics technologies for deconvoluting the tumor microenvironment
This article is protected by copyright. All rights reserved.PMID:34125481 | DOI:10.1002/biot.202100041 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 14, 2021 Category: Biotechnology Authors: Nan Wang Xia Li Rongshui Wang Zhiyong Ding Source Type: research

Fluorescence-based screening for engineered aldo-keto reductase KmAKR with improved catalytic performance and extended substrate scope
CONCLUSIONS: A fluorescence-based HT screening system was developed to tailor KmAKR's activity, thermostability and substrate scope. The "best" variant M5-Q213A/T23V holds great potential application for the synthesis of aliphatic/aromatic R-configuration alcohols. This article is protected by copyright. All rights reserved.PMID:34125995 | DOI:10.1002/biot.202100130 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 14, 2021 Category: Biotechnology Authors: Shuai Qiu Shen-Yuan Xu Shu-Fang Li Kang-Ming Meng Feng Cheng Ya-Jun Wang Yu-Guo Zheng Source Type: research

Spatial transcriptomics and proteomics technologies for deconvoluting the tumor microenvironment
This article is protected by copyright. All rights reserved.PMID:34125481 | DOI:10.1002/biot.202100041 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 14, 2021 Category: Biotechnology Authors: Nan Wang Xia Li Rongshui Wang Zhiyong Ding Source Type: research

Fluorescence-based screening for engineered aldo-keto reductase KmAKR with improved catalytic performance and extended substrate scope
CONCLUSIONS: A fluorescence-based HT screening system was developed to tailor KmAKR's activity, thermostability and substrate scope. The "best" variant M5-Q213A/T23V holds great potential application for the synthesis of aliphatic/aromatic R-configuration alcohols. This article is protected by copyright. All rights reserved.PMID:34125995 | DOI:10.1002/biot.202100130 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 14, 2021 Category: Biotechnology Authors: Shuai Qiu Shen-Yuan Xu Shu-Fang Li Kang-Ming Meng Feng Cheng Ya-Jun Wang Yu-Guo Zheng Source Type: research

Spatial transcriptomics and proteomics technologies for deconvoluting the tumor microenvironment
This article is protected by copyright. All rights reserved.PMID:34125481 | DOI:10.1002/biot.202100041 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 14, 2021 Category: Biotechnology Authors: Nan Wang Xia Li Rongshui Wang Zhiyong Ding Source Type: research

Prevention of nosocomial transmission and biofilm formation on novel biocompatible antimicrobial gloves impregnated with biosynthesized silver nanoparticles synthesized using Eucalyptus citriodora leaf extract
Biotechnol J. 2021 Jun 8:e2100030. doi: 10.1002/biot.202100030. Online ahead of print.ABSTRACTBACKGROUND: Failure in the prevention of cross-transmission from contaminated gloves has been recognized as an important factor that contributes to the spread of several healthcare-associated infections.METHODS AND RESULTS: Ex situ coating process with silver nanoparticles (AgNPs) using Eucalyptus citriodora ethanolic leaf extract as reducing and capping agents to coat glove surfaces has been developed to prevent this mode of transmission. Elemental analysis of coated gloves showed 24.8 Wt% silver densely adhere on the surface. Th...
Source: Biotechnology Journal - June 8, 2021 Category: Biotechnology Authors: Supakit Paosen Sakkarin Lethongkam Suttiwan Wunnoo Nussana Lehman Ekwipoo Kalkornsurapranee Abdi Wira Septama Supayang Piyawan Voravuthikunchai Source Type: research

Ribozyme-processed guide RNA enhances virus-mediated plant genome editing
This article is protected by copyright. All rights reserved.PMID:34102014 | DOI:10.1002/biot.202100189 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 8, 2021 Category: Biotechnology Authors: Youngbin Oh Hyeonjin Kim Hyo-Jun Lee Sang-Gyu Kim Source Type: research

Prevention of nosocomial transmission and biofilm formation on novel biocompatible antimicrobial gloves impregnated with biosynthesized silver nanoparticles synthesized using Eucalyptus citriodora leaf extract
Biotechnol J. 2021 Jun 8:e2100030. doi: 10.1002/biot.202100030. Online ahead of print.ABSTRACTBACKGROUND: Failure in the prevention of cross-transmission from contaminated gloves has been recognized as an important factor that contributes to the spread of several healthcare-associated infections.METHODS AND RESULTS: Ex situ coating process with silver nanoparticles (AgNPs) using Eucalyptus citriodora ethanolic leaf extract as reducing and capping agents to coat glove surfaces has been developed to prevent this mode of transmission. Elemental analysis of coated gloves showed 24.8 Wt% silver densely adhere on the surface. Th...
Source: Biotechnology Journal - June 8, 2021 Category: Biotechnology Authors: Supakit Paosen Sakkarin Lethongkam Suttiwan Wunnoo Nussana Lehman Ekwipoo Kalkornsurapranee Abdi Wira Septama Supayang Piyawan Voravuthikunchai Source Type: research

Ribozyme-processed guide RNA enhances virus-mediated plant genome editing
This article is protected by copyright. All rights reserved.PMID:34102014 | DOI:10.1002/biot.202100189 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 8, 2021 Category: Biotechnology Authors: Youngbin Oh Hyeonjin Kim Hyo-Jun Lee Sang-Gyu Kim Source Type: research

Prevention of nosocomial transmission and biofilm formation on novel biocompatible antimicrobial gloves impregnated with biosynthesized silver nanoparticles synthesized using Eucalyptus citriodora leaf extract
Biotechnol J. 2021 Jun 8:e2100030. doi: 10.1002/biot.202100030. Online ahead of print.ABSTRACTBACKGROUND: Failure in the prevention of cross-transmission from contaminated gloves has been recognized as an important factor that contributes to the spread of several healthcare-associated infections.METHODS AND RESULTS: Ex situ coating process with silver nanoparticles (AgNPs) using Eucalyptus citriodora ethanolic leaf extract as reducing and capping agents to coat glove surfaces has been developed to prevent this mode of transmission. Elemental analysis of coated gloves showed 24.8 Wt% silver densely adhere on the surface. Th...
Source: Biotechnology Journal - June 8, 2021 Category: Biotechnology Authors: Supakit Paosen Sakkarin Lethongkam Suttiwan Wunnoo Nussana Lehman Ekwipoo Kalkornsurapranee Abdi Wira Septama Supayang Piyawan Voravuthikunchai Source Type: research

Ribozyme-processed guide RNA enhances virus-mediated plant genome editing
This article is protected by copyright. All rights reserved.PMID:34102014 | DOI:10.1002/biot.202100189 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 8, 2021 Category: Biotechnology Authors: Youngbin Oh Hyeonjin Kim Hyo-Jun Lee Sang-Gyu Kim Source Type: research

Prevention of nosocomial transmission and biofilm formation on novel biocompatible antimicrobial gloves impregnated with biosynthesized silver nanoparticles synthesized using Eucalyptus citriodora leaf extract
Biotechnol J. 2021 Jun 8:e2100030. doi: 10.1002/biot.202100030. Online ahead of print.ABSTRACTBACKGROUND: Failure in the prevention of cross-transmission from contaminated gloves has been recognized as an important factor that contributes to the spread of several healthcare-associated infections.METHODS AND RESULTS: Ex situ coating process with silver nanoparticles (AgNPs) using Eucalyptus citriodora ethanolic leaf extract as reducing and capping agents to coat glove surfaces has been developed to prevent this mode of transmission. Elemental analysis of coated gloves showed 24.8 Wt% silver densely adhere on the surface. Th...
Source: Biotechnology Journal - June 8, 2021 Category: Biotechnology Authors: Supakit Paosen Sakkarin Lethongkam Suttiwan Wunnoo Nussana Lehman Ekwipoo Kalkornsurapranee Abdi Wira Septama Supayang Piyawan Voravuthikunchai Source Type: research

Ribozyme-processed guide RNA enhances virus-mediated plant genome editing
This article is protected by copyright. All rights reserved.PMID:34102014 | DOI:10.1002/biot.202100189 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 8, 2021 Category: Biotechnology Authors: Youngbin Oh Hyeonjin Kim Hyo-Jun Lee Sang-Gyu Kim Source Type: research

Prevention of nosocomial transmission and biofilm formation on novel biocompatible antimicrobial gloves impregnated with biosynthesized silver nanoparticles synthesized using Eucalyptus citriodora leaf extract
Biotechnol J. 2021 Jun 8:e2100030. doi: 10.1002/biot.202100030. Online ahead of print.ABSTRACTBACKGROUND: Failure in the prevention of cross-transmission from contaminated gloves has been recognized as an important factor that contributes to the spread of several healthcare-associated infections.METHODS AND RESULTS: Ex situ coating process with silver nanoparticles (AgNPs) using Eucalyptus citriodora ethanolic leaf extract as reducing and capping agents to coat glove surfaces has been developed to prevent this mode of transmission. Elemental analysis of coated gloves showed 24.8 Wt% silver densely adhere on the surface. Th...
Source: Biotechnology Journal - June 8, 2021 Category: Biotechnology Authors: Supakit Paosen Sakkarin Lethongkam Suttiwan Wunnoo Nussana Lehman Ekwipoo Kalkornsurapranee Abdi Wira Septama Supayang Piyawan Voravuthikunchai Source Type: research

Ribozyme-processed guide RNA enhances virus-mediated plant genome editing
This article is protected by copyright. All rights reserved.PMID:34102014 | DOI:10.1002/biot.202100189 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 8, 2021 Category: Biotechnology Authors: Youngbin Oh Hyeonjin Kim Hyo-Jun Lee Sang-Gyu Kim Source Type: research

Advances in production and structural derivatization of the promising molecule ursolic acid
CONCLUSION: In combination with the advantages of UA biosynthesis and transformation strategy, large-scale microbial production of UA is a promising platform for further exploration. This article is protected by copyright. All rights reserved.PMID:34096160 | DOI:10.1002/biot.202000657 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 7, 2021 Category: Biotechnology Authors: Hao-Ran Liu Nadeem Ahmad Bo Lv Chun Li Source Type: research

Advances in production and structural derivatization of the promising molecule ursolic acid
CONCLUSION: In combination with the advantages of UA biosynthesis and transformation strategy, large-scale microbial production of UA is a promising platform for further exploration. This article is protected by copyright. All rights reserved.PMID:34096160 | DOI:10.1002/biot.202000657 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 7, 2021 Category: Biotechnology Authors: Hao-Ran Liu Nadeem Ahmad Bo Lv Chun Li Source Type: research

Using lysozyme amyloid fibrils as a means of scavenging aggregation-inhibiting compounds
This article is protected by copyright. All rights reserved.PMID:34089232 | DOI:10.1002/biot.202100138 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 5, 2021 Category: Biotechnology Authors: Mantas Ziaunys Kamile Mikalauskaite Andrius Sakalauskas Vytautas Smirnovas Source Type: research

Microaerobic growth-decoupled production of α-ketoglutarate and succinate from xylose in a one-pot process using Corynebacterium glutamicum
CONCLUSION: The developed one-pot process represents a promising approach for the combined supply of bio-based α-ketoglutarate and succinate. Future work will focus on tailor-made down-stream processing of both organic acids from the fermentation broth to enable their application as building blocks in chemical syntheses. Alternatively, direct conversion of one or both acids via whole-cell or cell-free enzymatic approaches can be envisioned, thus extending the network of value chains starting from cheap and renewable d-xylose. This article is protected by copyright. All rights reserved.PMID:34089621 | DOI:10.1002/biot...
Source: Biotechnology Journal - June 5, 2021 Category: Biotechnology Authors: Niklas Tenhaef Jannick Kappelmann Arabel Eich Marc Weiske Lisette Brie ß Christian Br üsseler Jan Marienhagen Wolfgang Wiechert Stephan Noack Source Type: research

Using lysozyme amyloid fibrils as a means of scavenging aggregation-inhibiting compounds
This article is protected by copyright. All rights reserved.PMID:34089232 | DOI:10.1002/biot.202100138 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 5, 2021 Category: Biotechnology Authors: Mantas Ziaunys Kamile Mikalauskaite Andrius Sakalauskas Vytautas Smirnovas Source Type: research

Spinal cord regeneration: A brief overview of present scenario and a sneak peek into the future
This article is protected by copyright. All rights reserved.PMID:34080314 | DOI:10.1002/biot.202100167 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 3, 2021 Category: Biotechnology Authors: Ekta Srivastava Anamika Singh Ashok Kumar Source Type: research

Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation
CONCLUSION: These data suggest that we can control tyrosine sulfation by selecting CHO cell lines based on the expression level of TPST2/SLC35B2 or adding sodium chlorate in upstream production process. This article is protected by copyright. All rights reserved.PMID:34081410 | DOI:10.1002/biot.202100142 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 3, 2021 Category: Biotechnology Authors: Ren Liu Yixiao Zhang Amit Kumar Steven Huhn Laurie Hullinger Zhimei Du Source Type: research

Spinal cord regeneration: A brief overview of present scenario and a sneak peek into the future
This article is protected by copyright. All rights reserved.PMID:34080314 | DOI:10.1002/biot.202100167 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 3, 2021 Category: Biotechnology Authors: Ekta Srivastava Anamika Singh Ashok Kumar Source Type: research

Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation
CONCLUSION: These data suggest that we can control tyrosine sulfation by selecting CHO cell lines based on the expression level of TPST2/SLC35B2 or adding sodium chlorate in upstream production process. This article is protected by copyright. All rights reserved.PMID:34081410 | DOI:10.1002/biot.202100142 (Source: Biotechnology Journal)
Source: Biotechnology Journal - June 3, 2021 Category: Biotechnology Authors: Ren Liu Yixiao Zhang Amit Kumar Steven Huhn Laurie Hullinger Zhimei Du Source Type: research

Stable overexpression of mutated PTEN in Chinese hamster ovary cells enhances their performance and therapeutic antibody production
This article is protected by copyright. All rights reserved.PMID:34053183 | DOI:10.1002/biot.202000623 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 30, 2021 Category: Biotechnology Authors: Qin Zhou Yujie Zhang Xiaoxiang Lu Chang Wang Xinxin Pei Yafang Lu Cheng Cao Changzhi Xu Buchang Zhang Source Type: research

An improved lentiviral system for efficient expression and purification of β-defensins in mammalian cells
This study has laid the foundation for future studies to characterize function and mechanism of novel β-defensins. This article is protected by copyright. All rights reserved.PMID:34053189 | DOI:10.1002/biot.202100023 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 30, 2021 Category: Biotechnology Authors: Shiraz Ahmed Jiang Tao Miaochen Wang Yue Zhai Wenhai Liu Muthukumaran Jayachandran Chen Chu Shen Qu Jin Zhang Yonglian Zhang Zhaoliang Fei Source Type: research

Stable overexpression of mutated PTEN in Chinese hamster ovary cells enhances their performance and therapeutic antibody production
This article is protected by copyright. All rights reserved.PMID:34053183 | DOI:10.1002/biot.202000623 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 30, 2021 Category: Biotechnology Authors: Qin Zhou Yujie Zhang Xiaoxiang Lu Chang Wang Xinxin Pei Yafang Lu Cheng Cao Changzhi Xu Buchang Zhang Source Type: research

An improved lentiviral system for efficient expression and purification of β-defensins in mammalian cells
This study has laid the foundation for future studies to characterize function and mechanism of novel β-defensins. This article is protected by copyright. All rights reserved.PMID:34053189 | DOI:10.1002/biot.202100023 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 30, 2021 Category: Biotechnology Authors: Shiraz Ahmed Jiang Tao Miaochen Wang Yue Zhai Wenhai Liu Muthukumaran Jayachandran Chen Chu Shen Qu Jin Zhang Yonglian Zhang Zhaoliang Fei Source Type: research

Stable overexpression of mutated PTEN in Chinese hamster ovary cells enhances their performance and therapeutic antibody production
This article is protected by copyright. All rights reserved.PMID:34053183 | DOI:10.1002/biot.202000623 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 30, 2021 Category: Biotechnology Authors: Qin Zhou Yujie Zhang Xiaoxiang Lu Chang Wang Xinxin Pei Yafang Lu Cheng Cao Changzhi Xu Buchang Zhang Source Type: research

An improved lentiviral system for efficient expression and purification of β-defensins in mammalian cells
This study has laid the foundation for future studies to characterize function and mechanism of novel β-defensins. This article is protected by copyright. All rights reserved.PMID:34053189 | DOI:10.1002/biot.202100023 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 30, 2021 Category: Biotechnology Authors: Shiraz Ahmed Jiang Tao Miaochen Wang Yue Zhai Wenhai Liu Muthukumaran Jayachandran Chen Chu Shen Qu Jin Zhang Yonglian Zhang Zhaoliang Fei Source Type: research

Stable overexpression of mutated PTEN in Chinese hamster ovary cells enhances their performance and therapeutic antibody production
This article is protected by copyright. All rights reserved.PMID:34053183 | DOI:10.1002/biot.202000623 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 30, 2021 Category: Biotechnology Authors: Qin Zhou Yujie Zhang Xiaoxiang Lu Chang Wang Xinxin Pei Yafang Lu Cheng Cao Changzhi Xu Buchang Zhang Source Type: research

An improved lentiviral system for efficient expression and purification of β-defensins in mammalian cells
This study has laid the foundation for future studies to characterize function and mechanism of novel β-defensins. This article is protected by copyright. All rights reserved.PMID:34053189 | DOI:10.1002/biot.202100023 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 30, 2021 Category: Biotechnology Authors: Shiraz Ahmed Jiang Tao Miaochen Wang Yue Zhai Wenhai Liu Muthukumaran Jayachandran Chen Chu Shen Qu Jin Zhang Yonglian Zhang Zhaoliang Fei Source Type: research

Naming CHO cells for bio-manufacturing: Genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names
This article discusses the relevance of such names. We argue that they provide a false sense of identity. To substantiate this, we provide the long (and poorly recorded) history of CHO cells as well as their highly complex genetics. Finally, we suggest an alternative naming system for CHO cells which provides more relevant information. While the implementation of a new naming convention will require substantial discussions among members of the relevant community, it should improve interpretation and comparability between laboratories. This, in turn will help scientific communities and industrial users to attain and further...
Source: Biotechnology Journal - May 29, 2021 Category: Biotechnology Authors: MariaJ Wurm FlorianM Wurm Source Type: research

Substrate-activated expression of a biosynthetic pathway in Escherichia coli
CONCLUSION: This work demonstrated feed-activated pathway expression to be an attractive control strategy for more readily scalable microbial biosynthesis. This article is protected by copyright. All rights reserved.PMID:34050620 | DOI:10.1002/biot.202000433 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 29, 2021 Category: Biotechnology Authors: Cynthia Ni Kevin J Fox Kristala L J Prather Source Type: research

Naming CHO cells for bio-manufacturing: Genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names
This article discusses the relevance of such names. We argue that they provide a false sense of identity. To substantiate this, we provide the long (and poorly recorded) history of CHO cells as well as their highly complex genetics. Finally, we suggest an alternative naming system for CHO cells which provides more relevant information. While the implementation of a new naming convention will require substantial discussions among members of the relevant community, it should improve interpretation and comparability between laboratories. This, in turn will help scientific communities and industrial users to attain and further...
Source: Biotechnology Journal - May 29, 2021 Category: Biotechnology Authors: MariaJ Wurm FlorianM Wurm Source Type: research

Substrate-activated expression of a biosynthetic pathway in Escherichia coli
CONCLUSION: This work demonstrated feed-activated pathway expression to be an attractive control strategy for more readily scalable microbial biosynthesis. This article is protected by copyright. All rights reserved.PMID:34050620 | DOI:10.1002/biot.202000433 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 29, 2021 Category: Biotechnology Authors: Cynthia Ni Kevin J Fox Kristala L J Prather Source Type: research

Supplementation of critical amino acids improves glycerol and lactose uptake and enhances recombinant protein production in Escherichia coli
CONCLUSIONS AND IMPLICATIONS: We hypothesize that supplementation of critical AAs serves dual purpose: i) faster assimilation of carbon sources, and ii) combating metabolic stress arises due to AAs starvation. The substrate uptake and gene expression profiles demonstrate that AAs addition enhances glycerol and lactose assimilation due to overall improvement in their metabolism governed by global regulators of carbon metabolism. This article is protected by copyright. All rights reserved.PMID:34047499 | DOI:10.1002/biot.202100143 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 28, 2021 Category: Biotechnology Authors: Jashwant Kumar AshishS Chauhan JayaA Gupta AnuragS Rathore Source Type: research

Chitosan and silver nanoparticles are attractive auxin carriers: A comparative study on the adventitious rooting of microcuttings in apple rootstocks
This article is protected by copyright. All rights reserved.PMID:34028191 | DOI:10.1002/biot.202100046 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 24, 2021 Category: Biotechnology Authors: Serdar Korpayev Ay şe Karakeçili Hatice Dumano ğlu Salwa Ibrahim Ahmed Osman Source Type: research

Effect of vitamins and metal ions on productivity and charge heterogeneity of IgG1 expressed in CHO cells
This study aims to explore the impact of vitamins and metal ions on protein expression and post-translational modifications specifically charge heterogeneity. Biotin, choline chloride, D-calcium pantothenate, folic acid, pyridoxine hydrochloride, thiamine hydrochloride vitamins and Fe, Cu, Mg, Co, Zn, Mn, Ni metal ions were examined in this study. The results indicate that pyridoxine enhances productivity while Zn, Cu, Fe, Mn, and biotin impact charge heterogeneity. While, Fe, Mn and Ni enhance production of the acidic variants, Cu and biotin inhibit it. Zn reduces formation of basic variants while biotin enhances it. The ...
Source: Biotechnology Journal - May 24, 2021 Category: Biotechnology Authors: Neelesh Gangwar Rishabh Mishra Niharika Budholiya Anurag S Rathore Source Type: research

Chitosan and silver nanoparticles are attractive auxin carriers: A comparative study on the adventitious rooting of microcuttings in apple rootstocks
This article is protected by copyright. All rights reserved.PMID:34028191 | DOI:10.1002/biot.202100046 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 24, 2021 Category: Biotechnology Authors: Serdar Korpayev Ay şe Karakeçili Hatice Dumano ğlu Salwa Ibrahim Ahmed Osman Source Type: research

Effect of vitamins and metal ions on productivity and charge heterogeneity of IgG1 expressed in CHO cells
CONCLUSION: The results from this investigation could be used for process control so as to get consistent charge variant profile, in particular for biosimilars. This article is protected by copyright. All rights reserved.PMID:34028198 | DOI:10.1002/biot.202000464 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 24, 2021 Category: Biotechnology Authors: Neelesh Gangwar Rishabh Mishra Niharika Budholiya Anurag S Rathore Source Type: research

Chitosan and silver nanoparticles are attractive auxin carriers: A comparative study on the adventitious rooting of microcuttings in apple rootstocks
This article is protected by copyright. All rights reserved.PMID:34028191 | DOI:10.1002/biot.202100046 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 24, 2021 Category: Biotechnology Authors: Serdar Korpayev Ay şe Karakeçili Hatice Dumano ğlu Salwa Ibrahim Ahmed Osman Source Type: research

Effect of vitamins and metal ions on productivity and charge heterogeneity of IgG1 expressed in CHO cells
CONCLUSION: The results from this investigation could be used for process control so as to get consistent charge variant profile, in particular for biosimilars. This article is protected by copyright. All rights reserved.PMID:34028198 | DOI:10.1002/biot.202000464 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 24, 2021 Category: Biotechnology Authors: Neelesh Gangwar Rishabh Mishra Niharika Budholiya Anurag S Rathore Source Type: research

Chitosan and silver nanoparticles are attractive auxin carriers: A comparative study on the adventitious rooting of microcuttings in apple rootstocks
This article is protected by copyright. All rights reserved.PMID:34028191 | DOI:10.1002/biot.202100046 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 24, 2021 Category: Biotechnology Authors: Serdar Korpayev Ay şe Karakeçili Hatice Dumano ğlu Salwa Ibrahim Ahmed Osman Source Type: research

Effect of vitamins and metal ions on productivity and charge heterogeneity of IgG1 expressed in CHO cells
CONCLUSION: The results from this investigation could be used for process control so as to get consistent charge variant profile, in particular for biosimilars. This article is protected by copyright. All rights reserved.PMID:34028198 | DOI:10.1002/biot.202000464 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 24, 2021 Category: Biotechnology Authors: Neelesh Gangwar Rishabh Mishra Niharika Budholiya Anurag S Rathore Source Type: research

An integrated modular framework for modeling the effect of ammonium on the sialylation process of monoclonal antibodies produced by CHO cells
CONCLUSION: The presented model in this study provides a connection between extracellular ammonium and the monoclonal antibody sialylation process. This computational tool could help scientists to develop and formulate cell culture media. The model illustrated here can assist the researchers to select culture media that ensure consistent mAB sialylation. This article is protected by copyright. All rights reserved.PMID:34021707 | DOI:10.1002/biot.202100019 (Source: Biotechnology Journal)
Source: Biotechnology Journal - May 22, 2021 Category: Biotechnology Authors: Iman Shahidi Pour Savizi Ehsan Motamedian Nathan E Lewis Ioscani Jimenez Del Val Seyed Abbas Shojaosadati Source Type: research