Erythrocytes membrane fluidity changes induced by adenylyl cyclase cascade activation: study using fluorescence recovery after photobleaching
In this study, fluorescence recovery after photobleaching (FRAP) experiments were performed on RBC labeled by lipophilic fluorescent dye CM-DiI to evaluate the role of adenylyl cyclase cascade activation in changes of lateral diffusion of erythrocytes membrane lipids. Stimulation of adrenergic receptors with epinephrine (adrenaline) or metaproterenol led to the significant acceleration of the FRAP recovery, thus indicating an elevated membrane fluidity. The effect of the stimulation of protein kinase A with membrane-permeable analog of cAMP followed the same trend but was less significant. The observed effects are assumed ...
Source: European Biophysics Journal : EBJ - April 16, 2024 Category: Physics Authors: A N Semenov A E Lugovtsov S A Rodionov Eu G Maksimov A V Priezzhev E A Shirshin Source Type: research
Erythrocytes membrane fluidity changes induced by adenylyl cyclase cascade activation: study using fluorescence recovery after photobleaching
In this study, fluorescence recovery after photobleaching (FRAP) experiments were performed on RBC labeled by lipophilic fluorescent dye CM-DiI to evaluate the role of adenylyl cyclase cascade activation in changes of lateral diffusion of erythrocytes membrane lipids. Stimulation of adrenergic receptors with epinephrine (adrenaline) or metaproterenol led to the significant acceleration of the FRAP recovery, thus indicating an elevated membrane fluidity. The effect of the stimulation of protein kinase A with membrane-permeable analog of cAMP followed the same trend but was less significant. The observed effects are assumed ...
Source: European Biophysics Journal : EBJ - April 16, 2024 Category: Physics Authors: A N Semenov A E Lugovtsov S A Rodionov Eu G Maksimov A V Priezzhev E A Shirshin Source Type: research
Reaction of KHP with excess NaOH or TRIS as standard reactions for calibration of titration calorimeters from 0 to 60 °C
Eur Biophys J. 2024 Apr 13. doi: 10.1007/s00249-024-01705-z. Online ahead of print.ABSTRACTCalibration of titration calorimeters is an ongoing problem, particularly with calorimeters with reaction vessel volumes < 10 mL in which an electrical calibration heater is positioned outside the calorimetric vessel. Consequently, a chemical reaction with a known enthalpy change must be used to accurately calibrate these calorimeters. This work proposes the use of standard solutions of potassium acid phthalate (KHP) titrated into solutions of excess sodium hydroxide (NaOH) or excess tris(hydroxymethyl)aminomethane (TRIS) as stand...
Source: European Biophysics Journal : EBJ - April 13, 2024 Category: Physics Authors: Jason D Kenealey Margarida Bastos Zaid Assaf Guangyue Bai Wenqi Zhao Tyler Jarrard Colter Tower Lee D Hansen Source Type: research
Reaction of KHP with excess NaOH or TRIS as standard reactions for calibration of titration calorimeters from 0 to 60 °C
Eur Biophys J. 2024 Apr 13. doi: 10.1007/s00249-024-01705-z. Online ahead of print.ABSTRACTCalibration of titration calorimeters is an ongoing problem, particularly with calorimeters with reaction vessel volumes < 10 mL in which an electrical calibration heater is positioned outside the calorimetric vessel. Consequently, a chemical reaction with a known enthalpy change must be used to accurately calibrate these calorimeters. This work proposes the use of standard solutions of potassium acid phthalate (KHP) titrated into solutions of excess sodium hydroxide (NaOH) or excess tris(hydroxymethyl)aminomethane (TRIS) as stand...
Source: European Biophysics Journal : EBJ - April 13, 2024 Category: Physics Authors: Jason D Kenealey Margarida Bastos Zaid Assaf Guangyue Bai Wenqi Zhao Tyler Jarrard Colter Tower Lee D Hansen Source Type: research
Application of electron paramagnetic resonance spectroscopy for determining the relative nanoenvironment fluidity of polymeric micelles
In this study, we used electron paramagnetic resonance (EPR) spectroscopy to study free radical spin probe (5-doxylstearate methyl ester, 5-MDS, and 16-doxylstearic acid, 16-DS) behaviour in methoxy-poly(ethylene oxide)-poly(α-benzyl carboxylate-ε-caprolactone) (PEO-PBCL) and methoxy-poly(ethylene oxide)-poly(ε-caprolactone) (PEO-PCL) polymeric micelles. Spin probes provided information about the spectroscopic rotational correlation time (τ, s) and the spectroscopic partition parameter F. We hypothesized that spin probes would partition into the polymeric micelles, and these parameters would be calculated. The results ...
Source: European Biophysics Journal : EBJ - April 10, 2024 Category: Physics Authors: Lusine Tonoyan Sirazum Munira Afsaneh Lavasanifar Arno G Siraki Source Type: research
Application of electron paramagnetic resonance spectroscopy for determining the relative nanoenvironment fluidity of polymeric micelles
In this study, we used electron paramagnetic resonance (EPR) spectroscopy to study free radical spin probe (5-doxylstearate methyl ester, 5-MDS, and 16-doxylstearic acid, 16-DS) behaviour in methoxy-poly(ethylene oxide)-poly(α-benzyl carboxylate-ε-caprolactone) (PEO-PBCL) and methoxy-poly(ethylene oxide)-poly(ε-caprolactone) (PEO-PCL) polymeric micelles. Spin probes provided information about the spectroscopic rotational correlation time (τ, s) and the spectroscopic partition parameter F. We hypothesized that spin probes would partition into the polymeric micelles, and these parameters would be calculated. The results ...
Source: European Biophysics Journal : EBJ - April 10, 2024 Category: Physics Authors: Lusine Tonoyan Sirazum Munira Afsaneh Lavasanifar Arno G Siraki Source Type: research
Application of electron paramagnetic resonance spectroscopy for determining the relative nanoenvironment fluidity of polymeric micelles
In this study, we used electron paramagnetic resonance (EPR) spectroscopy to study free radical spin probe (5-doxylstearate methyl ester, 5-MDS, and 16-doxylstearic acid, 16-DS) behaviour in methoxy-poly(ethylene oxide)-poly(α-benzyl carboxylate-ε-caprolactone) (PEO-PBCL) and methoxy-poly(ethylene oxide)-poly(ε-caprolactone) (PEO-PCL) polymeric micelles. Spin probes provided information about the spectroscopic rotational correlation time (τ, s) and the spectroscopic partition parameter F. We hypothesized that spin probes would partition into the polymeric micelles, and these parameters would be calculated. The results ...
Source: European Biophysics Journal : EBJ - April 10, 2024 Category: Physics Authors: Lusine Tonoyan Sirazum Munira Afsaneh Lavasanifar Arno G Siraki Source Type: research
Design of inhibitor peptide sequences based on the interfacial knowledge of the protein G-IgG crystallographic complex and their binding studies with IgG
Eur Biophys J. 2024 Apr;53(3):159-170. doi: 10.1007/s00249-024-01704-0. Epub 2024 Mar 17.ABSTRACTProtein-protein interactions (PPI) have emerged as valuable targets in medicinal chemistry due to their key roles in important biological processes. The modulation of PPI by small peptides offers an excellent opportunity to develop drugs against human diseases. Here, we exploited the knowledge of the binding interface of the IgG-protein G complex (PDB:1FCC) for designing peptides that can inhibit these complexes. Herein, we have designed several closely related peptides, and the comparison of results from experiments and comput...
Source: European Biophysics Journal : EBJ - March 17, 2024 Category: Physics Authors: Neetu Tanwar Rupal Ojha Soumya Aggarwal Vijay Kumar Prajapati Manoj Munde Source Type: research
Design of inhibitor peptide sequences based on the interfacial knowledge of the protein G-IgG crystallographic complex and their binding studies with IgG
Eur Biophys J. 2024 Apr;53(3):159-170. doi: 10.1007/s00249-024-01704-0. Epub 2024 Mar 17.ABSTRACTProtein-protein interactions (PPI) have emerged as valuable targets in medicinal chemistry due to their key roles in important biological processes. The modulation of PPI by small peptides offers an excellent opportunity to develop drugs against human diseases. Here, we exploited the knowledge of the binding interface of the IgG-protein G complex (PDB:1FCC) for designing peptides that can inhibit these complexes. Herein, we have designed several closely related peptides, and the comparison of results from experiments and comput...
Source: European Biophysics Journal : EBJ - March 17, 2024 Category: Physics Authors: Neetu Tanwar Rupal Ojha Soumya Aggarwal Vijay Kumar Prajapati Manoj Munde Source Type: research
Design of inhibitor peptide sequences based on the interfacial knowledge of the protein G-IgG crystallographic complex and their binding studies with IgG
Eur Biophys J. 2024 Apr;53(3):159-170. doi: 10.1007/s00249-024-01704-0. Epub 2024 Mar 17.ABSTRACTProtein-protein interactions (PPI) have emerged as valuable targets in medicinal chemistry due to their key roles in important biological processes. The modulation of PPI by small peptides offers an excellent opportunity to develop drugs against human diseases. Here, we exploited the knowledge of the binding interface of the IgG-protein G complex (PDB:1FCC) for designing peptides that can inhibit these complexes. Herein, we have designed several closely related peptides, and the comparison of results from experiments and comput...
Source: European Biophysics Journal : EBJ - March 17, 2024 Category: Physics Authors: Neetu Tanwar Rupal Ojha Soumya Aggarwal Vijay Kumar Prajapati Manoj Munde Source Type: research
Design of inhibitor peptide sequences based on the interfacial knowledge of the protein G-IgG crystallographic complex and their binding studies with IgG
Eur Biophys J. 2024 Apr;53(3):159-170. doi: 10.1007/s00249-024-01704-0. Epub 2024 Mar 17.ABSTRACTProtein-protein interactions (PPI) have emerged as valuable targets in medicinal chemistry due to their key roles in important biological processes. The modulation of PPI by small peptides offers an excellent opportunity to develop drugs against human diseases. Here, we exploited the knowledge of the binding interface of the IgG-protein G complex (PDB:1FCC) for designing peptides that can inhibit these complexes. Herein, we have designed several closely related peptides, and the comparison of results from experiments and comput...
Source: European Biophysics Journal : EBJ - March 17, 2024 Category: Physics Authors: Neetu Tanwar Rupal Ojha Soumya Aggarwal Vijay Kumar Prajapati Manoj Munde Source Type: research
Design of inhibitor peptide sequences based on the interfacial knowledge of the protein G-IgG crystallographic complex and their binding studies with IgG
Eur Biophys J. 2024 Apr;53(3):159-170. doi: 10.1007/s00249-024-01704-0. Epub 2024 Mar 17.ABSTRACTProtein-protein interactions (PPI) have emerged as valuable targets in medicinal chemistry due to their key roles in important biological processes. The modulation of PPI by small peptides offers an excellent opportunity to develop drugs against human diseases. Here, we exploited the knowledge of the binding interface of the IgG-protein G complex (PDB:1FCC) for designing peptides that can inhibit these complexes. Herein, we have designed several closely related peptides, and the comparison of results from experiments and comput...
Source: European Biophysics Journal : EBJ - March 17, 2024 Category: Physics Authors: Neetu Tanwar Rupal Ojha Soumya Aggarwal Vijay Kumar Prajapati Manoj Munde Source Type: research
Design of inhibitor peptide sequences based on the interfacial knowledge of the protein G-IgG crystallographic complex and their binding studies with IgG
Eur Biophys J. 2024 Apr;53(3):159-170. doi: 10.1007/s00249-024-01704-0. Epub 2024 Mar 17.ABSTRACTProtein-protein interactions (PPI) have emerged as valuable targets in medicinal chemistry due to their key roles in important biological processes. The modulation of PPI by small peptides offers an excellent opportunity to develop drugs against human diseases. Here, we exploited the knowledge of the binding interface of the IgG-protein G complex (PDB:1FCC) for designing peptides that can inhibit these complexes. Herein, we have designed several closely related peptides, and the comparison of results from experiments and comput...
Source: European Biophysics Journal : EBJ - March 17, 2024 Category: Physics Authors: Neetu Tanwar Rupal Ojha Soumya Aggarwal Vijay Kumar Prajapati Manoj Munde Source Type: research
Design of inhibitor peptide sequences based on the interfacial knowledge of the protein G-IgG crystallographic complex and their binding studies with IgG
Eur Biophys J. 2024 Apr;53(3):159-170. doi: 10.1007/s00249-024-01704-0. Epub 2024 Mar 17.ABSTRACTProtein-protein interactions (PPI) have emerged as valuable targets in medicinal chemistry due to their key roles in important biological processes. The modulation of PPI by small peptides offers an excellent opportunity to develop drugs against human diseases. Here, we exploited the knowledge of the binding interface of the IgG-protein G complex (PDB:1FCC) for designing peptides that can inhibit these complexes. Herein, we have designed several closely related peptides, and the comparison of results from experiments and comput...
Source: European Biophysics Journal : EBJ - March 17, 2024 Category: Physics Authors: Neetu Tanwar Rupal Ojha Soumya Aggarwal Vijay Kumar Prajapati Manoj Munde Source Type: research
Design of inhibitor peptide sequences based on the interfacial knowledge of the protein G-IgG crystallographic complex and their binding studies with IgG
Eur Biophys J. 2024 Apr;53(3):159-170. doi: 10.1007/s00249-024-01704-0. Epub 2024 Mar 17.ABSTRACTProtein-protein interactions (PPI) have emerged as valuable targets in medicinal chemistry due to their key roles in important biological processes. The modulation of PPI by small peptides offers an excellent opportunity to develop drugs against human diseases. Here, we exploited the knowledge of the binding interface of the IgG-protein G complex (PDB:1FCC) for designing peptides that can inhibit these complexes. Herein, we have designed several closely related peptides, and the comparison of results from experiments and comput...
Source: European Biophysics Journal : EBJ - March 17, 2024 Category: Physics Authors: Neetu Tanwar Rupal Ojha Soumya Aggarwal Vijay Kumar Prajapati Manoj Munde Source Type: research
