Mesenchymal Stem Cell Derived-Exosomes as Effective Factors in Reducing Cytokine Storm Symptoms of COVID-19
Protein Pept Lett. 2021 Feb 22. doi: 10.2174/0929866528666210222150347. Online ahead of print.ABSTRACTGiven that conventional therapies are ineffective for COVID-19, obtained exosomes from stem cells have been proposed as a sustainable and effective treatment. Exosomes are subsets with lengths between 30 and 100 nanometers, and they can be secreted by different cells. Exosomes are containing different types of miRNAs, mRNAs, and different proteins. The role of immune system modulation of exosomes of mesenchymal stem cells has been studied and confirmed in more than one study. Exosome miRNAs detect and reduce cytokines that...
Source: Protein and Peptide Letters - February 23, 2021 Category: Biochemistry Authors: Amir Hossein Kheirkhah Seyed Hossein Shahcheraghi Malihe Lotfi Marzieh Lotfi Sanaz Raeisi Zulfiqar Mirani Source Type: research

Predicting Protein Surface Property with its Surface Hydrophobicity
This article reviews and discusses the relationship between surface hydrophobicity and other surface properties of proteins and the possibility of using surface hydrophobicity as a key indicator to predict and evaluate the changes in the surface properties of a protein. Hydrophobicity is the main driving force of protein folding; it affects the structure and functions. Surface hydrophobicity and other surface properties of proteins are controlled by their spatial structures. Due to the hydrophobic interactions, most proteins fold into their globular structures, and they lack sufficient hydrophobic residues on the molecular...
Source: Protein and Peptide Letters - February 23, 2021 Category: Biochemistry Authors: Sen Tang Junsheng Li Guoxia Huang Liujuan Yan Source Type: research

Mesenchymal Stem Cell Derived-Exosomes as Effective Factors in Reducing Cytokine Storm Symptoms of COVID-19
Protein Pept Lett. 2021 Feb 22. doi: 10.2174/0929866528666210222150347. Online ahead of print.ABSTRACTGiven that conventional therapies are ineffective for COVID-19, obtained exosomes from stem cells have been proposed as a sustainable and effective treatment. Exosomes are subsets with lengths between 30 and 100 nanometers, and they can be secreted by different cells. Exosomes are containing different types of miRNAs, mRNAs, and different proteins. The role of immune system modulation of exosomes of mesenchymal stem cells has been studied and confirmed in more than one study. Exosome miRNAs detect and reduce cytokines that...
Source: Protein and Peptide Letters - February 23, 2021 Category: Biochemistry Authors: Amir Hossein Kheirkhah Seyed Hossein Shahcheraghi Malihe Lotfi Marzieh Lotfi Sanaz Raeisi Zulfiqar Mirani Source Type: research

Predicting Protein Surface Property with its Surface Hydrophobicity
This article reviews and discusses the relationship between surface hydrophobicity and other surface properties of proteins and the possibility of using surface hydrophobicity as a key indicator to predict and evaluate the changes in the surface properties of a protein. Hydrophobicity is the main driving force of protein folding; it affects the structure and functions. Surface hydrophobicity and other surface properties of proteins are controlled by their spatial structures. Due to the hydrophobic interactions, most proteins fold into their globular structures, and they lack sufficient hydrophobic residues on the molecular...
Source: Protein and Peptide Letters - February 23, 2021 Category: Biochemistry Authors: Sen Tang Junsheng Li Guoxia Huang Liujuan Yan Source Type: research

Mesenchymal Stem Cell Derived-Exosomes as Effective Factors in Reducing Cytokine Storm Symptoms of COVID-19
Protein Pept Lett. 2021 Feb 22. doi: 10.2174/0929866528666210222150347. Online ahead of print.ABSTRACTGiven that conventional therapies are ineffective for COVID-19, obtained exosomes from stem cells have been proposed as a sustainable and effective treatment. Exosomes are subsets with lengths between 30 and 100 nanometers, and they can be secreted by different cells. Exosomes are containing different types of miRNAs, mRNAs, and different proteins. The role of immune system modulation of exosomes of mesenchymal stem cells has been studied and confirmed in more than one study. Exosome miRNAs detect and reduce cytokines that...
Source: Protein and Peptide Letters - February 23, 2021 Category: Biochemistry Authors: Amir Hossein Kheirkhah Seyed Hossein Shahcheraghi Malihe Lotfi Marzieh Lotfi Sanaz Raeisi Zulfiqar Mirani Source Type: research

Predicting Protein Surface Property with its Surface Hydrophobicity
This article reviews and discusses the relationship between surface hydrophobicity and other surface properties of proteins and the possibility of using surface hydrophobicity as a key indicator to predict and evaluate the changes in the surface properties of a protein. Hydrophobicity is the main driving force of protein folding; it affects the structure and functions. Surface hydrophobicity and other surface properties of proteins are controlled by their spatial structures. Due to the hydrophobic interactions, most proteins fold into their globular structures, and they lack sufficient hydrophobic residues on the molecular...
Source: Protein and Peptide Letters - February 23, 2021 Category: Biochemistry Authors: Sen Tang Junsheng Li Guoxia Huang Liujuan Yan Source Type: research

Mesenchymal Stem Cell Derived-Exosomes as Effective Factors in Reducing Cytokine Storm Symptoms of COVID-19
Protein Pept Lett. 2021 Feb 22. doi: 10.2174/0929866528666210222150347. Online ahead of print.ABSTRACTGiven that conventional therapies are ineffective for COVID-19, obtained exosomes from stem cells have been proposed as a sustainable and effective treatment. Exosomes are subsets with lengths between 30 and 100 nanometers, and they can be secreted by different cells. Exosomes are containing different types of miRNAs, mRNAs, and different proteins. The role of immune system modulation of exosomes of mesenchymal stem cells has been studied and confirmed in more than one study. Exosome miRNAs detect and reduce cytokines that...
Source: Protein and Peptide Letters - February 23, 2021 Category: Biochemistry Authors: Amir Hossein Kheirkhah Seyed Hossein Shahcheraghi Malihe Lotfi Marzieh Lotfi Sanaz Raeisi Zulfiqar Mirani Source Type: research

Predicting Protein Surface Property with its Surface Hydrophobicity
This article reviews and discusses the relationship between surface hydrophobicity and other surface properties of proteins and the possibility of using surface hydrophobicity as a key indicator to predict and evaluate the changes in the surface properties of a protein. Hydrophobicity is the main driving force of protein folding; it affects the structure and functions. Surface hydrophobicity and other surface properties of proteins are controlled by their spatial structures. Due to the hydrophobic interactions, most proteins fold into their globular structures, and they lack sufficient hydrophobic residues on the molecular...
Source: Protein and Peptide Letters - February 23, 2021 Category: Biochemistry Authors: Sen Tang Junsheng Li Guoxia Huang Liujuan Yan Source Type: research

Biotechnological Eminence of Chitinases: A Focus on Thermophilic Enzyme Sources, Production Strategies and Prominent Applications
CONCLUSION: This review will provide information about chitinases, biotechnological potential of thermostable enzyme and the methods by which they are being produced and optimized for several industrial applications. Some of the applications of thermostable chitinases have also been briefly described.PMID:33602064 | DOI:10.2174/0929866528666210218215359 (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - February 19, 2021 Category: Biochemistry Authors: Fatima Akram Rabia Akram None Ikram Ul Haq Ali Nawaz Zuriat Jabbar Zeeshan Ahmed Source Type: research

Beyond B-Cell Epitopes: Curating Positive Data on Antipeptide Paratope Binding to Support Peptide-Based Vaccine Design
CONCLUSION: The results suggest an alternative approach to curating paratope-epitope binding data based on neutralization of biological activity by polyclonal antipeptide antibodies, with reference to immunogenic peptide sequences and their conformational disorder in unbound antigen structures.PMID:33602065 | DOI:10.2174/0929866528666210218215624 (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - February 19, 2021 Category: Biochemistry Authors: Salvador Eugenio C Caoili Source Type: research

Potential Application of CRISPR/Cas9 System to Engineer Abiotic Stress Tolerance in Plants
Protein Pept Lett. 2021 Feb 18. doi: 10.2174/0929866528666210218220138. Online ahead of print.ABSTRACTAbiotic stresses in plants such as salinity, drought, heavy metal toxicity, heat, and nutrients limitations significantly reduce agricultural production worldwide. The genome editing techniques such as transcriptional activator-like effector nucleases (TALENs) and zinc finger nucleases (ZFNs) have been used for genome manipulations in plants. However, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technique has recently emerged as a promising tool for genome editing in...
Source: Protein and Peptide Letters - February 19, 2021 Category: Biochemistry Authors: Temoor Ahmed Muhammad Shahid Muhammad Noman Sher Muhammad Muhammad Tahir Ul Qamar Md Arshad Ali Awais Maqsood Rahila Hafeez Solabomi Olaitan Ogunyemi Bin Li Source Type: research

Biotechnological Eminence of Chitinases: A Focus on Thermophilic Enzyme Sources, Production Strategies and Prominent Applications.
CONCLUSION: This review will provide information about chitinases, biotechnological potential of thermostable enzyme and the methods by which they are being produced and optimized for several industrial applications. Some of the applications of thermostable chitinases have also been briefly described. PMID: 33602064 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - February 18, 2021 Category: Biochemistry Authors: Akram F, Akram R, Ikram Ul Haq, Nawaz A, Jabbar Z, Ahmed Z Tags: Protein Pept Lett Source Type: research

Beyond B-Cell Epitopes: Curating Positive Data on Antipeptide Paratope Binding to Support Peptide-Based Vaccine Design.
CONCLUSION: The results suggest an alternative approach to curating paratope-epitope binding data based on neutralization of biological activity by polyclonal antipeptide antibodies, with reference to immunogenic peptide sequences and their conformational disorder in unbound antigen structures. PMID: 33602065 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - February 18, 2021 Category: Biochemistry Authors: C Caoili SE Tags: Protein Pept Lett Source Type: research

Potential Application of CRISPR/Cas9 System to Engineer Abiotic Stress Tolerance in Plants.
Abstract Abiotic stresses in plants such as salinity, drought, heavy metal toxicity, heat, and nutrients limitations significantly reduce agricultural production worldwide. The genome editing techniques such as transcriptional activator-like effector nucleases (TALENs) and zinc finger nucleases (ZFNs) have been used for genome manipulations in plants. However, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technique has recently emerged as a promising tool for genome editing in plants to acquire desirable traits. The CRISPR/Cas9 system has a great potential to...
Source: Protein and Peptide Letters - February 18, 2021 Category: Biochemistry Authors: Ahmed T, Shahid M, Noman M, Muhammad S, Tahir Ul Qamar M, Ali MA, Maqsood A, Hafeez R, Ogunyemi SO, Li B Tags: Protein Pept Lett Source Type: research

Phosphoserine aminotransferase has conserved active site from microbes to higher eukaryotes with minor deviations.
Abstract Serine is ubiquitously synthesized in all living organisms from the glycolysis intermediate 3-phosphoglycerate (PGA) by phosphoserine biosynthetic pathway, consisting of three different enzymes, namely: 3-phosphoglycerate dehydrogenase (PGDH), phosphoserine aminotransferase (PSAT), and phosphoserine phosphatase (PSP). Any functional defect or mutation in these enzymes may cause deliberating conditions, such as colon cancer progression and chemoresistance in humans. Phosphoserine aminotransferase (PSAT) is the second enzyme in this pathway that converts phosphohydroxypyruvate (PHP) to O-phospho-L-serine (O...
Source: Protein and Peptide Letters - February 15, 2021 Category: Biochemistry Authors: Singh RK, Kumar D, Gourinath S Tags: Protein Pept Lett Source Type: research

Genome-Wide Association Study for major Biofuel Traits in Sorghum Using Minicore Collection.
In this study, the GWAS was performed on 245 sorghum minicore genotypes to analyze agronomic traits (days to 50%flowering, fresh biomass yield, dry biomass yield) and cell wall components (cellulose, hemicellulose, and lignin). Further, in-silico validation of the candidate genes was performed in a global gene expression data from large-scale RNA sequencing studies in sorghum available in the NCBI GEO database was used. OBJECTIVE: The objectives of this study are to evaluate native variations in biofuel related agronomic traits and stalk cell wall components and to identify significant SNPs or loci related to the cell...
Source: Protein and Peptide Letters - February 15, 2021 Category: Biochemistry Authors: Rayaprolu L, Selvanayagam S, Daggu DMR, Gupta R, Das RR, Rathore A, Gandham P, Kiranmayee KU, Deshpande SP, Are AK Tags: Protein Pept Lett Source Type: research

A Flounder Fish Peptide Shows Anti-Hypertensive Effects by Suppressing the Renin-Angiotensin-Aldosterone System and Endothelin-1.
CONCLUSION: Our results show that the MEVFVP has an antihypertensive effect by regulating renin-angiotensin-aldosterone system, ET-1 and AMPK despite its limited bioavailability. PMID: 33573539 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - February 11, 2021 Category: Biochemistry Authors: Rahmdel M, Cho SM, Jeon YJ, Lee DH Tags: Protein Pept Lett Source Type: research

nSite-Selective, Chemical Modification of Protein at Aromatic Side Chain and Their Emergent Applications.
Abstract Site-selective chemical modification of protein side chain has probed enormous opportunities in the fundamental understanding of cellular biology and therapeutic applications. Primarily, in the field of biopharmaceutical where formulation of bioconjugates is found to be potential medicine than an individual constituent. In this regard, Lysine and Cysteine are the most widely used endogenous amino acid for these purposes. Recently, the aromatic side chain residues (Trp, Tyr, and His) that are low abundant in protein have gained more attention in therapeutic applications due to their advantages of chemical ...
Source: Protein and Peptide Letters - January 29, 2021 Category: Biochemistry Authors: Chowdhury A, Chatterjee S, Pongen A, Sarania D, Tripathi NM, Bandyopadhyay A Tags: Protein Pept Lett Source Type: research

Characterization and Expression Analysis of Resistance Gene Analogues in Elite Sugarcane Genotypes.
CONCLUSION: RGAs are present in most of our indigenous genotypes. Anyhow, differential expression of five RGAs indicated that they have some critical role in disease resistance. So, the retrieved results can not only be employed to devise molecular markers for the screening of disease resistant genotypes but can also be used to develop disease resistant plants through transgenic technology. PMID: 33511939 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - January 29, 2021 Category: Biochemistry Authors: Parvaiz A, Mustafa G, Khan MS, Ali MA Tags: Protein Pept Lett Source Type: research

Hydroalcoholic Extract of Solanum lycocarpum A. St. Hil. (Solanaceae) Leaves Improves Alloxan-Induced Diabetes Complications in Mice.
s SHS Abstract BACKGROUND: Solanum lycocarpum is a medicinal plant widely-used in Brazil because its fruits have hypoglycemic activity. However, the fruits are restricted in some periods of the year. OBJECTIVE: To evaluate the effects of hydroalcoholic extracts of S. lycocarpum leaves in alloxan-induced diabetic mice. METHODS: Hydroalcoholic extract of S. lycocarpum was characterized by phytochemical and GC-MS analysis. The Antidiabetic activity was assessed following treatment for 22 days with S. lycocarpum extract at 125, 250, and 500 mg/kg. Bodyweight, water, and food intake, glycemia, biochemical par...
Source: Protein and Peptide Letters - January 28, 2021 Category: Biochemistry Authors: Guimarãesa VHD, Basilio Silva JN, de Freitas DF, Filho OC, da Silveira LH, Marinho BM, de Paula AMB, Melo GA, Santos SHS Tags: Protein Pept Lett Source Type: research

Plant terpenes on treating cardiovascular and metabolic disease: a review.
Abstract The use of medicinal plants as a therapy alternative is old as human existence itself. Nowadays, the search for effective molecules for chronic diseases treatments has increased. The cardiometabolic disorders still the main cause of death worldwide and plants may offer potential pharmacological innovative approaches to treat and prevent diseases. In the range of plant molecules are inserted the terpenes, which constituent essential elements with several pharmacological characteristics and applications, including cardiovascular and metabolic properties. Thus, the aim of the present review is to update the ...
Source: Protein and Peptide Letters - January 28, 2021 Category: Biochemistry Authors: Oliveira JR, Ribeiro GHM, Rezende LF, Fraga-Silva RA Tags: Protein Pept Lett Source Type: research

Plastid Transcriptomics: An Important Tool For Plastid Functional Genomics.
Abstract Plastids in higher plants carry out specialized roles such as photosynthesis, nitrogen assimilation, biosynthesis of amino acids, fatty acids, isoprenoids, and various metabolites. Plastids arise from undifferentiated precursors known as proplastids, which are found in the root and shoot meristems. They are highly dynamic as they change their number, morphology, and physiology according to the tissue they are present. In addition to housing various metabolic activities, plastids also serve as a global sensor for both internal and external environmental cues including different stresses, and help plants to...
Source: Protein and Peptide Letters - January 28, 2021 Category: Biochemistry Authors: Ahmad N, Nielsen BL Tags: Protein Pept Lett Source Type: research

Hepatotoxic Effect of Lafoensia pacari A. St. Hil. (Lythraceae) on a DietInduced Obese Mice Model.
CONCLUSION: We conclude that Lafoensia pacari should be better evaluated for oral consumption and may cause liver damage. PMID: 33504292 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - January 27, 2021 Category: Biochemistry Authors: Ribeiro NG, Dos Santos FR, Oliveira JR, Machado AS, de Faria Lelis D, de Paula AMB, Guimarães ALS, Farias LC, Santos SHS Tags: Protein Pept Lett Source Type: research

Review of Evidence and Perspectives of Flavonoids on Metabolic Syndrome and Neurodegenerative Disease.
Abstract Flavonoids are commonly found in fruits, vegetables, and plant-derived foods and may promote various health benefits when included in the diet. The biological activity of flavonoids is normally associated to their potent antioxidant and anti-inflammatory effects, since oxidative stress is associated to conditions such as diabetes, obesity, cardiovascular and neurodegenerative diseases. Additionally, flavonoids may be related to metabolic diseases through their effects on inflammatory mediators and pathways, barrier integrity and gut microbiota composition. The extensive metabolism undergone by flavonoids ...
Source: Protein and Peptide Letters - January 27, 2021 Category: Biochemistry Authors: Zilli AMH, Zilli EM Tags: Protein Pept Lett Source Type: research

Genome-Wide Association Studies (GWAS) for Traits Related to Fodder Quality and Biofuel in Sorghum: Progress and Prospects.
Abstract The main focus of this review is to discuss the current status of the use of GWAS for fodder quality and biofuel owing to its similarity of traits. Sorghum is a potential multipurpose crop, popularly cultivated for various uses as food, feed fodder, and biomass for ethanol. Production of a huge quantity of biomass and genetic variation for complex sugars are the main motivation not only to use sorghum as fodder for livestock nutritionists but also a potential candidate for biofuel generation. Few studies have been reported on the knowledge transfer that can be used from the development of biofuel technolo...
Source: Protein and Peptide Letters - January 27, 2021 Category: Biochemistry Authors: Somegowda VK, Rayaprolu L, Rathore A, Deshpande SP, Gupta R Tags: Protein Pept Lett Source Type: research

Metabolomics: A Powerful Tool to Study the Complexity of Wheat Metabolome.
Abstract Wheat is a widely cultivated cereal, consumed by nearly 80% of the total population in the world. Although wheat is growing on 215 million hectares annually, its production is still inadequate to meet the future demand of feeding the 10 billion human population. Global food security is the biggest challenge as climate change is threatening crop production. There is a need to fast-track the wheat breeding by devising modern biotechnological tools. Climate-smart wheat having greater stress resilience, better adaptability and improved agronomic traits are vital to guarantee food security. Substantial underst...
Source: Protein and Peptide Letters - January 27, 2021 Category: Biochemistry Authors: Razzaq A, Guul W, Khan MS, Saleem F Tags: Protein Pept Lett Source Type: research

From secretion in Pichia pastoris to application in apple juice processing: Exo-polygalacturonase from Sporothrix schenckii 1099-18.
CONCLUSION: The clarification potential of SsExo-PG was revealed by comparing it with commercial pectinolytic enzymes. The following parameters of the process of apple juice clarification processes showed that SsExo-PG is highly stable and has a novel performance. PMID: 33413052 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - January 5, 2021 Category: Biochemistry Authors: Karataş E, Tülek A, Çakar MM, Tamtürk F, Aktaş F, Binay B Tags: Protein Pept Lett Source Type: research

HIV-1 Accessory Proteins: Which one is Potentially Effective in Diagnosis and Vaccine Development?
CONCLUSION: Generally, among accessory proteins, Nef, Vpu and Vif antigens were potentially more suitable vaccine antigen candidates than Vpr antigen. Human antibodies against all these proteins were higher in HIV-1 different groups than healthy group. Among them, Vpu was known as a potent antigen in diagnosis of treated from untreated individuals. The potency of accessory proteins as an antigen candidate in an animal model and a human cohort study are underway. PMID: 33390106 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - December 31, 2020 Category: Biochemistry Authors: Milani A, Baesi K, Agi E, Marouf G, Ahmadi M, Bolhassani A Tags: Protein Pept Lett Source Type: research

Assessment of Proteolysis by Pyrylium and Other Fluorogenic Reagents.
CONCLUSION: These results are confirming reliability of highly sensitive protein assays utilizing amine reactive fluorogenic reagents for general estimation of proteolysis. PMID: 33390107 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - December 31, 2020 Category: Biochemistry Authors: Al-Essa MK, Alzayadneh E, Al-Hadidi K Tags: Protein Pept Lett Source Type: research

Facile synthesis of Boc-protected selenocystine and its compatibility with late-stage farnesylation at cysteine site.
Abstract BACKGROUND: The unique hypervariable C-terminal region (HVR) of K-Ras4B, one of the most frequently mutated proteins in many powerful cancers, contains a C-terminal farnesylated and methylated Cys and a poly-lysine motif, which decides the association of K-Ras4B to the inner leaflet of plasma membrane for activating the downstream signaling activity. In our previous work, we inserted an additional Cys in K-Ras4B HVR peptide synthesis for NCL in the semisynthesis of K-Ras4b protein, but it is not suitable for application in protein dimerization research. The recently developed selenocysteine (Sec, U) media...
Source: Protein and Peptide Letters - December 22, 2020 Category: Biochemistry Authors: Zhu PC, Chen YX Tags: Protein Pept Lett Source Type: research

Protein and Peptide Markers in Endocrine Diseases.
PMID: 33292109 [PubMed - in process] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - December 11, 2020 Category: Biochemistry Authors: Ferlin A, Grande G Tags: Protein Pept Lett Source Type: research

DeepSSPred: A Deep Learning Based Sulfenylation site predictor via a novel n-segmented optimize federated feature encoder.
CONCLUSION: In this research, we have developed a novel sequence-based automated predictor for SC-sites, called DeepSSPred. The empirical simulations outcomes with a training dataset and independent validation dataset have revealed the efficacy of the proposed theoretical model. The good performance of DeepSSPred is due to several reasons, such as novel discriminative feature encoding schemes, SMOTE technique, and careful construction of the prediction model through the tuned 2D-CNN classifier. We believe that our research work will provide a potential insight into a further prediction of S-sulfenylation characteristics an...
Source: Protein and Peptide Letters - December 1, 2020 Category: Biochemistry Authors: Khan ZU, Pi D Tags: Protein Pept Lett Source Type: research

Identification of binding partners of CsaA - an archaeal chaperonic pro-tein of Picrophilus torridus.
CONCLUSION: PtCsaA might be a multifaceted protein which besides translation might also play important role in metabolic processes of P. torridus. However, further experiments investigating the binding partners of CsaA in other archaea are re-quired for a better understanding of CsaA-associated processes in archaea. PMID: 33243110 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 26, 2020 Category: Biochemistry Authors: Singhal N, Sharma A, Aswal M, Singh N, Kumar M, Goel M Tags: Protein Pept Lett Source Type: research

Kinetic and Thermodynamic Study of Plantaricin IIA-1A5, a Bacteriocin Produced by Indonesian Probiotic Lactobacillus plantarum IIA-1A5.
CONCLUSION: This result showed that plantaricin IIA-1A5 is considerably more heat-stable than plantaricin members and promises to be applied in food industries where heat treatments are applied. Furthermore, a possible mechanism by which plantaricin IIA-1A5 maintains its stability was also discussed by referring to its thermodynamic parameters. PMID: 33231143 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 23, 2020 Category: Biochemistry Authors: Arifin M, Budiman C, Fujiyama K, Arief II Tags: Protein Pept Lett Source Type: research

Eco-Friendly Synthesis of Peptides Using Fmoc-Amino Acid Chlorides as Coupling Agent Under Biphasic Condition.
CONCLUSION: The present method developed greener using natural agro-waste (lemon fruit shell ash) derived solvent medium for the reaction and not required chemical entity. PMID: 33213309 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 19, 2020 Category: Biochemistry Authors: Khatavi SY, Kantharaju K Tags: Protein Pept Lett Source Type: research

N-Terminally Added Tag Selectively Enhances Heterologous Expression of VacA Cytotoxin Variants from Helicobacter pylori.
CONCLUSION: Our data provides molecular insights into selective influence of the N-terminally added tag on efficient expression of recombinant VacA variants, signifying biochemical and biological implications of the hydrophobic stretch within the N-terminal domain. PMID: 33183185 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 12, 2020 Category: Biochemistry Authors: Linn AK, Samainukul N, Sakdee S, Butnampetch C, Li HC, Angsuthanasombat C, Katzenmeier G Tags: Protein Pept Lett Source Type: research

Biochemical study of fibrinolytic protease from Euphausia superba possessing multifunctional serine protease activity.
CONCLUSION: Our study indicated that EFP has an essential serine residue for its catalyst function and associated folding behaviors. Also, the functional role of osmolytes such as proline and glycine that may play a role in defense mechanisms from environmental adaptation in a krill's body was suggested. PMID: 33183186 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 12, 2020 Category: Biochemistry Authors: Qian GY, Lim G, Yin SJ, Yang JM, Lee J, Park YD Tags: Protein Pept Lett Source Type: research

Evaluation of the In Vivo Acute Toxicity and In Vitro Hemolytic and Immunomodulatory Activities of the Moringa oleifera Flower Trypsin Inhibitor (MoFTI).
CONCLUSION: These results contribute to the ongoing evaluation of the antitumor potential of MoFTI and its effects on other immunological targets. PMID: 33191881 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 12, 2020 Category: Biochemistry Authors: de Siqueira Patriota LL, do Nascimento Santos DKD, da Silva Barros BR, de Souza Aguiar LM, Silva YA, Dos Santos ACLA, Gama E Silva M, Barroso Coelho LCB, Paiva PMG, Pontual EV, de Melo CML, Mendes RL, Napoleão TH Tags: Protein Pept Lett Source Type: research

Soluble Expression, One-Step Purification and Characterization of Recombinant Human Growth Hormone Fused with ompA3 in Escherichia coli.
CONCLUSION: Our research provides a feasible and convenient method for the soluble expression of recombinant hGH in E. coli, and may lay a foundation for the production and application of hGH in the industry. PMID: 33172365 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 10, 2020 Category: Biochemistry Authors: Zhou ZR, Huang W, Liu KJ, Lin FL, Wang XL, Wang F, Jiang R Tags: Protein Pept Lett Source Type: research

The PentaFOLD 3.0 algorithm for the selection of stable elements of secondary structure to be included in vaccine peptides.
CONCLUSION: The PentaFOLD 3.0 algorithm (http://chemres.bsmu.by/PentaFOLD30.htm) can be used with the aim to design vaccine peptides with stable secondary structure elements. PMID: 33172366 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 10, 2020 Category: Biochemistry Authors: Khrustalev VV Tags: Protein Pept Lett Source Type: research

EnzyPha, an Engineered Helper Phage Developed to Overcome Most of the Limitations Regarding Filamentous Phage Titration and ELISA Tests.
CONCLUSION: The results showed that the proposed model would titrate phages more sensitive than plating titration method through a shorter timeframe. Moreover, it could be a better alternative to the routine phage ELISA due to time saving, cost effective and higher sensitivity. PMID: 33172367 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 10, 2020 Category: Biochemistry Authors: Mohammadi M, Fouladvand F, Bemani P, Amini R, Jalilian FA Tags: Protein Pept Lett Source Type: research

Simultaneous optimization of activity and stability of xylose reductase from D. nepalensis NCYC 3413 using statistical experimental design.
CONCLUSION: Simultaneous optimization of xylose reductase activity and stability using statistical methods is effective as compared to optimisation of the parameters separately. PMID: 33143604 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 3, 2020 Category: Biochemistry Authors: Malla S, Gummadi SN Tags: Protein Pept Lett Source Type: research

Variable Length Character N-Gram Embedding of Protein Sequences for Secondary Structure Prediction.
CONCLUSION: The proposed model performance compares with state-of-the-art methods available in the literature. After a comparative analysis, it observed that the proposed model performs better than state-of-the-art methods. PMID: 33143605 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 3, 2020 Category: Biochemistry Authors: Sharma AK, Srivastava R Tags: Protein Pept Lett Source Type: research

Carbonic Anhydrase Carrying Electrospun Nanofibers for Biocatalysis Applications.
CONCLUSION: After applying the framework, we get a new biocatalysis application platform for carbonic anhydrase enzyme. Electrospun nanofibers were chosen as the support material for enzyme immobilization. By using this approach, the carbonic anhydrase enzyme could easily be used in the industrial area by cost-effective advantageous aspects. PMID: 33143606 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 3, 2020 Category: Biochemistry Authors: Ünlüer ÖB, Ecevit K, Diltemiz SE Tags: Protein Pept Lett Source Type: research

Purification of Classical Swine Fever Virus E2 Subunit Vaccines basing on High Affinity Peptide Ligand.
CONCLUSION: The computational-designed peptide in this study has high sensitivity and selectivity and is thus useful for the purification of CSFV E2. The novel method of design provided a broad platform and powerful tool for protein-peptide screening, as well as new insights into CSFV vaccine design. PMID: 33143607 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 3, 2020 Category: Biochemistry Authors: Wang F, Yu Q, Hu M, Xing G, Zhao D, Zhang G Tags: Protein Pept Lett Source Type: research

Conformational Dynamics Analysis of MEK1 Using Hydrogen/Deuterium Exchange Mass Spectrometry.
CONCLUSION: We report for the first time that MEK1 has regions that slowly change its folded and unfolded states (mixed EX1/EX2 kinetics) and also report the conformational effects of ATP-binding to MEK1. PMID: 33143608 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 3, 2020 Category: Biochemistry Authors: Yun MW, Kim K, Park JY, Chung KY Tags: Protein Pept Lett Source Type: research

A Thermostable Aluminum-tolerant Protease Produced by FeatherDegrading Bacillus thuringiensis Isolated from Tea Plantation.
This study aimed to analyze the properties by biochemical and enzymological methods to gain information for better utilization of the enzyme. METHODS: The enzyme was purified with ion exchange and size exclusion chromatography. The substrate preference, optimal pH and temperature, and the effects of organic solvents and ions were checked. Circular dichroism was performed to compare the secondary structures of the native and apo-enzyme. RESULTS: The enzyme worked best at 50 o C, and it was an acidic serine protease with an optimal pH of 6.2. Ions Ca2+ and Mg2+ were essential for its activity. Organic solvents and ...
Source: Protein and Peptide Letters - November 3, 2020 Category: Biochemistry Authors: Wang T, Liang C, Xiao S, Li L, Xu H, An Y, Zheng M, Liu L Tags: Protein Pept Lett Source Type: research

A Novel Reversibly Glycosylated Polypeptide-2 of Bee Pollen from Rape (Brassica napus L.): Purification and Characterization.
CONCLUSION: RGP2 has a highly conserved sequence of amino acid residues and potential glycosylation sites. PMID: 33143610 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - November 3, 2020 Category: Biochemistry Authors: Zhang Q, Sun T, Tuo X, Li Y, Yang H, Deng J Tags: Protein Pept Lett Source Type: research

Identification of Peptide Binders to Truncated Recombinant Chikungunya Virus Envelope Protein 2 Using Phage Display Technology and Their In Silico Characterization.
CONCLUSION: The potential binding peptides of CHIKV-rE2 protein were identified using phage display technology and characterized in silico. The selected peptides could be further used for the development of therapeutics against the CHIKV infection. PMID: 33121397 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - October 29, 2020 Category: Biochemistry Authors: Agarwal G, Gabrani R Tags: Protein Pept Lett Source Type: research

Evaluation of the Cardioprotective Effect of Granulocyte Colony Stimulating Factor in Patients with Carbon Monoxide Poisoning.
CONCLUSION: GCSF could probably reduce CO-induced cardiac ischemia in patients with acute CO poisoning. PMID: 33092501 [PubMed - as supplied by publisher] (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - October 22, 2020 Category: Biochemistry Authors: Pourtaji A, Sahebkar A, Poorzand H, Moshiri M, Mohammadpour AH, Mousavi SR Tags: Protein Pept Lett Source Type: research