Characterization of Heat-labile Uracil-DNA Glycosylase from Oncorhynchus mykiss and its Application for Carry-over Contamination Control in RT-qPCR
CONCLUSION: We successfully identified and recombinantly expressed a novel HL-UDG with an inactivation temperature of 40°C. It is suitable for eliminating carry-over contamination in one-step RT-qPCR.PMID:38343045 | DOI:10.2174/0109298665283737240122105923 (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - February 12, 2024 Category: Biochemistry Authors: Qingyuan Huang Yaqi Zhang Wenhao Hu Keqi Chen Jian Zhang Zhidan Luo Chen Lu Source Type: research
Characterization of Heat-labile Uracil-DNA Glycosylase from Oncorhynchus mykiss and its Application for Carry-over Contamination Control in RT-qPCR
CONCLUSION: We successfully identified and recombinantly expressed a novel HL-UDG with an inactivation temperature of 40°C. It is suitable for eliminating carry-over contamination in one-step RT-qPCR.PMID:38343045 | DOI:10.2174/0109298665283737240122105923 (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - February 12, 2024 Category: Biochemistry Authors: Qingyuan Huang Yaqi Zhang Wenhao Hu Keqi Chen Jian Zhang Zhidan Luo Chen Lu Source Type: research
Prokaryotic Expression and Affinity Purification of DDX3 Protein
CONCLUSION: A prokaryotic expression pNIC28-DDX3 system is constructed for efficient expression and affinity purification of bioactive DDX3 protein in E. coli BL21(DE3), which provides an important high-throughput screening and validation of drugs targeting DDX3.PMID:38303525 | DOI:10.2174/0109298665285625231222075700 (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - February 2, 2024 Category: Biochemistry Authors: Lan Huang Yue Liang Huijin Hou Min Tang Xinpeng Liu Yan-Ni Ma Shufang Liang Source Type: research
Characterization of a Novel Thermostable 7 α-hydroxysteroid Dehydrogenase
CONCLUSION: We characterized the thermostable 7α-HSDH, which provides a promising biocatalyst for bioconversion of steroids at high reaction temperatures.PMID:38288819 | DOI:10.2174/0109298665279004231229100320 (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - January 30, 2024 Category: Biochemistry Authors: Deshuai Lou Yangyang Cao Hongtao Duan Jun Tan Binyan Li Yuanjun Zhou Dong Wang Source Type: research
P53-induced GAP-43 Upregulation in Primary Cortical Neurons of Rats
CONCLUSION: Within primary rat cortical neurons, p53 has the potential to prompt an augmentation in both the transcriptional and protein expression levels of the GAP-43 protein.PMID:38288820 | DOI:10.2174/0109298665263864231221071712 (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - January 30, 2024 Category: Biochemistry Authors: Tianxia Li Yuexin Jia Junxian Fu Zhuo Fu Zhidong Qiao Xiaoyang Liu Ting Lv Rong Tang Guanglu Yang Source Type: research
Molecular Machinery of the Triad Holin, Endolysin, and Spanin: Key Players Orchestrating Bacteriophage-Induced Cell Lysis and their Therapeutic Applications
Protein Pept Lett. 2024 Jan 22. doi: 10.2174/0109298665181166231212051621. Online ahead of print.ABSTRACTPhage therapy has attracted attention as a possible alternative treatment for multi-drug resistance (MDR) infections in recent years. The lytic bacteriophages encode proteins for bacterial host envelope rupture. Phages produce endolysins muralytic enzymes, they are phage-encoded peptidoglycan hydrolases (PGHs) that cause enzymatically breakdown of the host bacterium's peptidoglycan (PG) or murein layer at the end of their lytic multiplication cycle. Phage holins regulate endolysin access to the PG, starting the lysis pr...
Source: Protein and Peptide Letters - January 23, 2024 Category: Biochemistry Authors: Safia Samir Source Type: research
Feasibility of Domain Segmentation of B19V VP1u Using Intein Technology for Structural Studies
CONCLUSION: This method would allow for the selective incorporation of NMR-active isotopes into either of the VP1u domains, which can reduce signal overlap in NMR structural determination studies.PMID:38243925 | DOI:10.2174/0109298665277211231214065419 (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - January 20, 2024 Category: Biochemistry Authors: Renuk Varayil Lakshmanan Mavis Agbandje-McKenna Robert McKenna Source Type: research
Improving Photocleavage Efficiency of Photocleavable Protein for Antimicrobial Peptide Histatin 1 Expression
CONCLUSION: Antimicrobial peptides Histatin 1, β -Defensin 105 and Lysostaphin were successfully expressed and purified by photocleavable protein mutant. This may provide a novel strategy to express and purify antimicrobial peptides in the Escherichia coli expression system.PMID:38243926 | DOI:10.2174/0109298665276722231212053009 (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - January 20, 2024 Category: Biochemistry Authors: Nana Zhou Tai An Yuan Zhang Guomiao Zhao Chao Wei Xuemei Shen Fan Li Xiaoyan Wang Source Type: research
Advances in Research on Protein Arginine Methyltransferase 2: Functions and Diseases
Protein Pept Lett. 2023 Dec 28. doi: 10.2174/0109298665281395231211060535. Online ahead of print.ABSTRACTProtein arginine methylation stands as a prevalent post-translational modification process, exerting vital roles in cellular signal transduction, gene expression, and cell cycle regulation. Amidst the protein arginine methyltransferase (PRMT) family, PRMT2 stands as a less explored constituent. Nonetheless, its regulatory roles in transcriptional regulation, post-transcriptional modification, methylation activity regulation, immunoregulation, and developmental regulation have garnered attention. These capabilities enabl...
Source: Protein and Peptide Letters - December 29, 2023 Category: Biochemistry Authors: Zhen-Qi Min Ming-Jun Jiang Xi-Lian Liu Su-Peng Yuan Ping-An Chen Chu-Hao Wang Ya-Jun Chen Xian-Peng Dai Source Type: research
Advances in Research on Protein Arginine Methyltransferase 2: Functions and Diseases
Protein Pept Lett. 2023 Dec 28. doi: 10.2174/0109298665281395231211060535. Online ahead of print.ABSTRACTProtein arginine methylation stands as a prevalent post-translational modification process, exerting vital roles in cellular signal transduction, gene expression, and cell cycle regulation. Amidst the protein arginine methyltransferase (PRMT) family, PRMT2 stands as a less explored constituent. Nonetheless, its regulatory roles in transcriptional regulation, post-transcriptional modification, methylation activity regulation, immunoregulation, and developmental regulation have garnered attention. These capabilities enabl...
Source: Protein and Peptide Letters - December 29, 2023 Category: Biochemistry Authors: Zhen-Qi Min Ming-Jun Jiang Xi-Lian Liu Su-Peng Yuan Ping-An Chen Chu-Hao Wang Ya-Jun Chen Xian-Peng Dai Source Type: research
Advances in Research on Protein Arginine Methyltransferase 2: Functions and Diseases
Protein Pept Lett. 2023 Dec 28. doi: 10.2174/0109298665281395231211060535. Online ahead of print.ABSTRACTProtein arginine methylation stands as a prevalent post-translational modification process, exerting vital roles in cellular signal transduction, gene expression, and cell cycle regulation. Amidst the protein arginine methyltransferase (PRMT) family, PRMT2 stands as a less explored constituent. Nonetheless, its regulatory roles in transcriptional regulation, post-transcriptional modification, methylation activity regulation, immunoregulation, and developmental regulation have garnered attention. These capabilities enabl...
Source: Protein and Peptide Letters - December 29, 2023 Category: Biochemistry Authors: Zhen-Qi Min Ming-Jun Jiang Xi-Lian Liu Su-Peng Yuan Ping-An Chen Chu-Hao Wang Ya-Jun Chen Xian-Peng Dai Source Type: research
Advances in Research on Protein Arginine Methyltransferase 2: Functions and Diseases
Protein Pept Lett. 2023 Dec 28. doi: 10.2174/0109298665281395231211060535. Online ahead of print.ABSTRACTProtein arginine methylation stands as a prevalent post-translational modification process, exerting vital roles in cellular signal transduction, gene expression, and cell cycle regulation. Amidst the protein arginine methyltransferase (PRMT) family, PRMT2 stands as a less explored constituent. Nonetheless, its regulatory roles in transcriptional regulation, post-transcriptional modification, methylation activity regulation, immunoregulation, and developmental regulation have garnered attention. These capabilities enabl...
Source: Protein and Peptide Letters - December 29, 2023 Category: Biochemistry Authors: Zhen-Qi Min Ming-Jun Jiang Xi-Lian Liu Su-Peng Yuan Ping-An Chen Chu-Hao Wang Ya-Jun Chen Xian-Peng Dai Source Type: research
Advances in Research on Protein Arginine Methyltransferase 2: Functions and Diseases
Protein Pept Lett. 2023 Dec 28. doi: 10.2174/0109298665281395231211060535. Online ahead of print.ABSTRACTProtein arginine methylation stands as a prevalent post-translational modification process, exerting vital roles in cellular signal transduction, gene expression, and cell cycle regulation. Amidst the protein arginine methyltransferase (PRMT) family, PRMT2 stands as a less explored constituent. Nonetheless, its regulatory roles in transcriptional regulation, post-transcriptional modification, methylation activity regulation, immunoregulation, and developmental regulation have garnered attention. These capabilities enabl...
Source: Protein and Peptide Letters - December 29, 2023 Category: Biochemistry Authors: Zhen-Qi Min Ming-Jun Jiang Xi-Lian Liu Su-Peng Yuan Ping-An Chen Chu-Hao Wang Ya-Jun Chen Xian-Peng Dai Source Type: research
Advances in Research on Protein Arginine Methyltransferase 2: Functions and Diseases
Protein Pept Lett. 2023 Dec 28. doi: 10.2174/0109298665281395231211060535. Online ahead of print.ABSTRACTProtein arginine methylation stands as a prevalent post-translational modification process, exerting vital roles in cellular signal transduction, gene expression, and cell cycle regulation. Amidst the protein arginine methyltransferase (PRMT) family, PRMT2 stands as a less explored constituent. Nonetheless, its regulatory roles in transcriptional regulation, post-transcriptional modification, methylation activity regulation, immunoregulation, and developmental regulation have garnered attention. These capabilities enabl...
Source: Protein and Peptide Letters - December 29, 2023 Category: Biochemistry Authors: Zhen-Qi Min Ming-Jun Jiang Xi-Lian Liu Su-Peng Yuan Ping-An Chen Chu-Hao Wang Ya-Jun Chen Xian-Peng Dai Source Type: research
Advances in Research on Protein Arginine Methyltransferase 2: Functions and Diseases
Protein Pept Lett. 2023 Dec 28. doi: 10.2174/0109298665281395231211060535. Online ahead of print.ABSTRACTProtein arginine methylation stands as a prevalent post-translational modification process, exerting vital roles in cellular signal transduction, gene expression, and cell cycle regulation. Amidst the protein arginine methyltransferase (PRMT) family, PRMT2 stands as a less explored constituent. Nonetheless, its regulatory roles in transcriptional regulation, post-transcriptional modification, methylation activity regulation, immunoregulation, and developmental regulation have garnered attention. These capabilities enabl...
Source: Protein and Peptide Letters - December 29, 2023 Category: Biochemistry Authors: Zhen-Qi Min Ming-Jun Jiang Xi-Lian Liu Su-Peng Yuan Ping-An Chen Chu-Hao Wang Ya-Jun Chen Xian-Peng Dai Source Type: research
