Characterization of Heat-labile Uracil-DNA Glycosylase from Oncorhynchus mykiss and its Application for Carry-over Contamination Control in RT-qPCR

CONCLUSION: We successfully identified and recombinantly expressed a novel HL-UDG with an inactivation temperature of 40°C. It is suitable for eliminating carry-over contamination in one-step RT-qPCR.PMID:38343045 | DOI:10.2174/0109298665283737240122105923

Source: Protein and Peptide Letters - February 12, 2024 Category: Biochemistry Authors: Qingyuan Huang Yaqi Zhang Wenhao Hu Keqi Chen Jian Zhang Zhidan Luo Chen Lu Source Type: research