Stature estimation in the South-East Asian population: A systematic review.
CONCLUSION: The most widely used regression equations for stature estimation in South-East Asian population were from the Thailand population. Further research is recommended to develop regression equations for other South-East Asian countries. PMID: 31427544 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Screening for microsatellite instability in colorectal carcinoma: Practical utility of immunohistochemistry and PCR with fragment analysis in a diagnostic histopathology setting.
Authors: Cheah PL, Li J, Looi LM, Koh CC, Lau TP, Chang SW, Teoh KH, Mun KS, Nazarina AR Abstract Since 2014, the National Comprehensive Cancer Network (NCCN) has recommended that colorectal carcinoma (CRC) be universally tested for high microsatellite instability (MSI-H) which is present in 15% of such cancers. Fidelity of resultant microsatellites during DNA replication is contingent upon an intact mismatch repair (MMR) system and lack of fidelity can result in tumourigenesis. Prior to commencing routine screening for MSI-H, we assessed two commonly used methods, immunohistochemical (IHC) determination of loss of...
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Characterisation of immunogenotypes of diffuse large B-cell lymphoma.
Authors: Phang KC, Hussin NH, Abdul Rahman F, Tizen NMS, Mansoor A, Masir N Abstract INTRODUCTION: Diffuse large B-cell lymphoma (DLBCL) is the most common aggressive type of non-Hodgkin lymphoma with variable clinical outcomes. The immunogenotypic features of this heterogeneous disease in Malaysia were not well characterized. MATERIALS & METHODS: In total 141 local series of DLBCL cases from UKM Medical Centre were retrospectively studied. RESULTS: Of these cases, we classified our patients into two subtypes: 32.7% (37/113) GCB and non-GCB 67.3% (76/113) by Hans algorithm and the results showed strong ...
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Androgen receptor expression in triple negative breast carcinoma and its association with the clinicopathological parameters.
CONCLUSION: AR is expressed in about one-third of TNBC and loss of AR immunoexpression does not predict adverse clinical outcomes. Larger cohorts for better characterisation of the role of AR immunoexpression in TNBC are warranted. PMID: 31427547 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

A comparison study of HER2 protein overexpression and its gene status in breast cancer.
Authors: Md Pauzi SH, Saari HN, Roslan MR, Syed Khair Azman Jamalulil SNS, Tauan IS, Mohd Rusli FA, Aizuddin AN Abstract INTRODUCTION: Evaluation of HER2 status in breast cancer using immunohistochemistry (IHC) and in-situ-hybridisation (ISH) study is important to establish prognosis and to select patient for targeted therapy. OBJECTIVE: The study aims to determine the concordance between HER2 protein IHC score and its gene status by dual-colour dual-hapten in-situ-hybridization (DDISH) study. MATERIALS AND METHODS: Retrospective study was performed on 767 referred breast cancer cases over a period of five ...
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Molecular detection of Enterobacteriaceae isolates producing blaOXA-48 and blaOXA-181 genes: A single centre study.
CONCLUSIONS: The prevalence of OXA-48 and OXA-181 CRE among all Enterobacteriaceae isolates in our institution is 0.069% and 0.008%, respectively. Nevertheless, our findings suggest that OXA-48 and OXA-181 carbapenemases appear to be important and possibly under-recognised causes of carbapenem resistance in Malaysia. PMID: 31427549 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

FISH versus real-time quantitative PCR for monitoring of minimal residual disease in chronic myeloid leukaemia patients on tyrosine kinase inhibitor therapy.
In this study, we compared FISH with QRT-PCR for detection of residual disease in CML. MATERIALS AND METHODS: CML patients on tyrosine kinase inhibitor (TKI) therapy and on regular follow up at University Kebangsaan Malaysia Medical Center (UKMMC) were selected. A comparative study was conducted between FISH and QRT-PCR for BCR-ABL transcripts at diagnosis and during follow-up. RESULTS: There was good correlation between FISH and QRT-PCR for BCR-ABL. At 6th month of follow-up post diagnosis, FISH had a sensitivity of 83.3% and specificity of 65.2% (k>0.339, p0.286, p 0.642, p
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

An Audit on Overnight Transfusion Practice in A Teaching Hospital in Malaysia.
Authors: Tang YL, Yousuf R, Wan Nawawi WM, Rahman IL, Zainal Abidin J, Rechard Nathan VR, Leong CF Abstract INTRODUCTION: Overnight transfusion (OT) is the blood transfusion taking place from 9pm to 8am. During this period, patients are exposed to increased risk of errors. This cross-sectional study aims to determine the incidence and practice of OT in Universiti Kebangsaan Malaysia Medical Centre. MATERIALS & METHODS: Data from all OT in June and mid-July 2017 were collected from recipients' cards, transfusion request forms and patient's case files, regarding discipline involved, indications, time interval...
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Major depressive disorder patients on antidepressant treatments display higher number of regulatory T cells.
In this study, we set out to investigate the percentage and absolute count of Tregs in major depressive disorder (MDD) patients and their correlation with disease severity. MATERIALS & METHODS: This is a case-control study consisting of 47 MDD patients and 47 healthy controls. MDD patients were treated with antidepressant drugs according to their physician's choice. The severity of MDD was assessed using Beck Depression Inventory (BDI) and Montgomery-Asberg Depression Rating Scale (MADRS) at the time of recruitment. Healthy controls completed the Depression Anxiety Scoring System (DASS21) questionnaire to ensure th...
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Association of coronary artery calcium score with calcification and degree of stenosis: An autopsy study.
This study aims to determine the association between coronary artery PMCT CS with histologically observed calcification and degree of stenosis of coronary arteries in post-mortem cases. MATERIALS & METHODS: This was a cross-sectional study involving 101 subjects recruited from the National Institute of Forensic Medicine (IPFN) Hospital Kuala Lumpur (HKL) over a period of 15 months, from December 2012 until April 2014. PMCT CS of the coronary arteries was calculated using Agatston-Janowitz score. Histological presence of calcification was observed and the degree of stenosis was calculated using an image analysis tec...
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Epidemiology, spectrum of clinical manifestations and diagnostic issue of acquired haemophilia: A case series.
Authors: Wan Ab Rahman WS, Abdullah WZ, Husin A, Nik Mohd Hassan NFF, Hassan MN, Zulkafli Z Abstract INTRODUCTION: Acquired haemophilia A (AHA) is a rare acquired bleeding disorder caused by polyclonal immunoglobulin G autoantibodies against clotting factor VIII (FVIII). The incidence was reported to be rare occurring in 0.2- 4 cases/million/year. Patients may present with different clinical manifestations to various specialties. Early recognition of the disease contributes to favourable clinical outcome. CASE SERIES: Here, we reported five cases of this disorder with different clinical presentations from two t...
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Acinic cell carcinoma of the parotid gland: A diagnostic dilemma on cytology.
We report cytomorphological features of two uncommon variants of acinic cell carcinoma. The first case was an eleven-year-old female with a nodular mass in parotid and the FNA smears demonstrated a lymphoepithelial lesion composed of epithelial tumour cells with features of acinar cells in a lymphoid background. The second case was a 62-year-old male with a large parotid mass. The FNA smears revealed presence of extracellular, acellular amyloid-like material with tumour cells arranged in follicles. DISCUSSION: Awareness of cytomorphological features of these unusual variants of acinic cell carcinoma may help to avoid d...
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Extranodal NK/T-cell lymphoma mimicking Crohn's colitis.
We report a case of a 46-year-old Chinese male who presented with six months history of abdominal pain, weight loss and rectal bleeding. Diagnostic colonoscopy revealed multiple aphthous ulcers within the ileo-caecal region and distal transverse colon, separated by normal mucosa, mimicking skip lesions of Crohn's colitis. Computer topography (CT) scan of the abdomen showed multiple circumferential thickenings involving predominantly the right colon. A clinical diagnosis of colonic Crohn's disease with possible perforation was made. An extended right hemicolectomy was performed due to uncontrolled rectal bleeding. Histopath...
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Nasopharyngeal undifferentiated carcinoma with sarcomatoid features: Pitfalls in the immunohistochemistry.
We present a case of an undifferentiated subtype of non-keratinizing squamous cell carcinoma (NK-SCC) with sarcomatoid features in the nasopharynx in a 69-year-old man who was difficult to diagnose due to spindle-shaped malignant cells. He was admitted because of a right nasal obstruction and right headache, and imaging revealed a heterogeneously enhanced irregularly shaped mass at the nasopharynx. Histopathologically, the tumour was partially organised, and the tumour cells were epithelioid or spindle-shaped. Initially, we erroneously diagnosed the tumour as an angiosarcoma owing to its false-negative immunoreaction for c...
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Intraductal Carcinoma of Salivary Gland Originating from an Intraparotid Lymph Node: A Case Report.
We present the second case of IDC originating from an intraparotid lymph node (LN) with a more detailed description of the histogenesis, immunohistochemistry (IHC) and updated molecular information. CASE REPORT: An 87-year-old male had a tumour nodule over the left parotid tail for about 20 years. Physical examinations revealed a 4.5 cm soft, non-tender and fixed mass. After the left parotidectomy, pathology confirmed the diagnosis of IDC arising within an intraparotid lymph node. The cystic component of the tumour was lined by single to multilayered ductal cells with micropapillary growth pattern. The solid part showe...
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

ALK-positive anaplastic large cell lymphoma with a monomorphic small-cell pattern masquerading as inflammatory gastric lesions.
We report a case of primary systemic ALK+ ALCL with a SC pattern that presented as secondary gastric lesions with a mixed LH and SC pattern that was masqueraded as inflammatory lesions. CASE REPORT: A 34-year-old woman with intractable epigastric pain was referred to have repeated endoscopy with biopsy. She was found to multiple gastric erosions and nodules that were diagnosed as inflammatory lesions both endoscopically and histologically. Meanwhile, she developed an acute onset of severe back pain associated with a pathologic compression fracture in the T3 thoracic vertebral body. Imaging studies disclosed a dissemina...
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Disseminated histoplasmosis mimicking an acute appendicitis.
We describe a case of a 33 years old gentleman who was clinically diagnosed as acute appendicitis at initial presentation in view of a one-week history of fever, right lower quadrant abdominal pain- and guarding at right iliac fossa. He had thrombocytopenia and lymphopenia on presentation. Mesenteric lymphadenitis and small bowel lesion were found intraoperatively, which was respectively biopsied and resected. Histopathological result confirms disseminated histoplasmosis. Retroviral screen was positive. He was treated with amphotericin B for one week, subsequently switched to oral itraconazole, followed by initiation of hi...
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Ulcerated bleeding palisaded encapsulated neuroma of the tongue.
This article describes a case of a 41-year-old male, a chronic smoker with an actively bleeding, ulcerated, solitary, firm lesion on the lateral border of the tongue which had bled thrice before. A differential diagnosis of pyogenic granuloma, haemangioma, fibroma, nerve sheath tumour, salivary gland tumour and malignancy was made and surgically excised. Histopathology of the excised specimen revealed a well-circumscribed lesion with spindle-shaped cells arranged in interlacing fascicles and with the help of immunohistochemical markers confirmed it to be a PEN. DISCUSSION: To our knowledge, this is the first descriptio...
Source: Malaysian Journal of Pathology - August 21, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Rapid 'technological lock in' and pathology - Is technology moving too fast?
Authors: Moorchung N Abstract The term "Lock In" as applied to Science and Technology refers to a technology which has been utilised for a certain amount of time and it has been determined that the technology is viable and cost effective. An analysis of the technological advancements in pathology over a period of time shows that the newer technologies in contrast to the older technologies are reaching a state of "Technological Lock In" much faster. Three different discoveries, the development of the autopsy as a research tool, the discovery of the microscope and immunohistochemistry illustrate h...
Source: Malaysian Journal of Pathology - April 28, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Reference ranges for D-dimer levels in Malaysian women in the three trimesters of pregnancy.
This study was carried out in pregnant Malaysian women in order to build applicable reference ranges for D-dimer. MATERIALS AND METHODS: A cross-sectional study was conducted to measure D-dimer in healthy pregnant women, and a non-pregnant control group, using the quantitative HaemosIL D-dimer HS500 assay. Reference ranges were derived using CLSI 'Robust' methods, and differences between group medians were tested using the Kruskal-Wallis and Mann-Whitney U tests. RESULTS: Plasma D-dimer levels were measured in 92 pregnant women (distributed across the three trimesters)and 31 control women. The medians (and referenc...
Source: Malaysian Journal of Pathology - April 28, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Inter-observer variability in the histologic criteria of diagnosis of hydatidiform moles.
CONCLUSION: There was moderate to substantial agreement rate between two pathologists in identification of two essential histologic criteria for diagnosis of molar pregnancies i.e. "hydropic change" and "trophoblastic proliferation". PMID: 31025633 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - April 28, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Long-term type 1 diabetes mellitus creates a pro-atherogenic environment conducive to early atherosclerosis in rats.
Authors: Vântu A, Ghertescu D, Fiscă C, Mărginean A, Hutanu A, Gheban D, Perian M, Serban RC, Scridon A Abstract INTRODUCTION: Experimental models are essential for clarifying the pathogenesis of atherosclerosis in the context of diabetes mellitus (DM). We aimed to evaluate the presence and the magnitude of several factors known to promote atherogenesis, and to assess the potential of a pro-atherogenic environment to stimulate the development of atherosclerotic lesions in a rat model of long-term type 1 DM. MATERIALS AND METHODS: Six control and five DM Wistar rats were evaluated. DM was induced at 11 w...
Source: Malaysian Journal of Pathology - April 28, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Expression of IGFBP-rP1 in ovarian and breast cancers in association with diabetes mellitus status.
Authors: Mohd Nafi SN, Siti Azrin AH, Mat Zin AA, Othman NH, Che Jalil NA Abstract INTRODUCTION: Insulin-like growth factor binding protein-related protein 1 (IGFBP-rP1) is an important component of the IGF system that regulates insulin resistance-related to tumour development. The aim of this study is to investigate the expression of IGFBP-rP1 among female cancer patients who are known or not known to have Type 2 Diabetes Mellitus (T2DM). MATERIALS AND METHODS: Using a cross-sectional design, cases of ovarian and breast cancer with clinical status of T2DM were selected over a 10-year period in Hospital Univers...
Source: Malaysian Journal of Pathology - April 28, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Ultrastructural aspects of sylvatic dengue virus infection in Vero cell.
CONCLUSIONS: This is the first ultrastructural study on sylvatic DENV and this comparative study revealed the similarities and differences of cellular responses and morphogenesis of two dengue ecotypes in vitro. The study revealed the weaker infectivity of sylvatic DENV in the surrogate model of enzootic host, which supposed to support better replication of enzootic DENV than endemic DENV. PMID: 31025636 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - April 28, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Syringocystadenoma papilliferum arising in a naevus sebaceous.
We report a case of syringocystadenoma papilliferum arising in an undiagnosed pre-existing naevus sebaceus in a 56-year-old female. PMID: 31025637 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - April 28, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Sudden cardiac death in a young adult man due to spontaneous coronary artery dissection.
We present a case of a 28-year-old Indian male with no previous medical illness who complained sudden onset of chest pain prior to his death. Autopsy revealed a left anterior descending coronary artery dissection associated with plaque rupture. The anterior wall of left ventricle showed contraction band necrosis. There was also atheroma present in the right coronary artery which was insignificant. Histologically, dissection was associated with atherosclerosis. There was no evidence of vasculitis. The cause of death was given as coronary artery dissection due to coronary artery atherosclerosis. PMID: 31025638 [PubMed - ...
Source: Malaysian Journal of Pathology - April 28, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Severe anti-D haemolytic disease of fetal and newborn in rhesus D negative primigravida.
DISCUSSION: The thorough antenatal ABO and RhD blood grouping with antibody screening is mandatory as part of prevention and early detection of HDFN especially due to anti-D alloimmunisation. Improper management of RhD negative women might lead to severe HDFN including in primigravida. PMID: 31025639 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - April 28, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Oesophageal hepatoid carcinoma with liver metastasis, a diagnostic dilemma.
We reported a case of oesophageal hepatoid carcinoma with multiple liver metastasis, that was associated with an increased serum AFP. The distinction between HCC and HC is important because HC is more aggressive and has a poorer prognosis with limited therapeutic options. An extensive diagnostic work-up which include a thorough clinical history, radiological investigations (computed tomography or magnetic resonance imaging) as well as tissue biopsy supported by a panel of immunohistochemical markers are necessary to aid in the diagnosis of HC. PMID: 31025640 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - April 28, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Diffuse large B-cell lymphoma of the small intestine in a patient with refractory coeliac disease.
We report a 54-year-old Caucasian man who presented with chronic diarrhoea and weight loss. He was diagnosed with coeliac disease based on positive serology results and duodenal, jejunal, and ileal biopsies that showed villous atrophy. Despite adherence to a gluten-free diet, there was no clinical remission and enteropathy-associated T cell lymphoma was suspected. Repeated endoscopic biopsy showed persistent mucosal disease but no evidence of lymphoma. Several weeks later he presented with a perforated jejunum. Histology of the resected jejunum showed diffuse infiltration of submucosa and muscularis propria by malignant ly...
Source: Malaysian Journal of Pathology - April 28, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

CNS germinoma with extensive calcification: An unusual histologic finding.
We describe a 13-year-old male with pineal region germinoma demonstrating extensive intratumoral calcification. CASE REPORT: He presented with worsening headache that was associated with fatigue, nausea and vomiting. Radiologic examination revealed a multilobular mass in the pineal region with internal calcifications. Biopsy showed a pure germinoma with unusually extensive calcification. DISCUSSION: Although a diagnosis may be suggested with a careful evaluation of imaging, there is no pathognomonic pattern. Thus, histologic verification is necessary for most pineal region masses. PMID: 31025642 [PubMed - in pr...
Source: Malaysian Journal of Pathology - April 28, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Congenital cytomegalic inclusion disease with disseminated Herpes simplex infection.
We report a case of congenital cytomegalovirus and Herpes simplex virus infection suspected via ultrasound indicated by the presence of fetal cerebral abnormalities. The pregnancy was electively terminated at 31 weeks of gestation. The postmortem examination of the foetus showed brain with lissencephaly. The histopathological examination revealed numerous enlarged cells containing cytomegalic inclusions and multinucleated giant cells in multiple fetal organs and placenta. Documented evidence of histopathological detection of cytomegalovirus inclusions in multiple organs are very sparse in literature. This case highlights t...
Source: Malaysian Journal of Pathology - April 28, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Lymph node angioma: Capsule or hilar angioma?
Authors: Handra-Luca A Abstract No abstract available. PMID: 31025644 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - April 28, 2019 Category: Pathology Tags: Malays J Pathol Source Type: research

Gardnerella vaginalis in perinatology: An overview of the clinicopathological correlation.
Authors: Wong YP, Tan GC, Wong KK, Anushia S, Cheah FC Abstract Gardnerella vaginalis (GV) is a facultatively anaerobic gram-variable bacillus and is the major organism involved in bacterial vaginosis. GV-associated bacterial vaginosis has been associated with adverse pregnancy outcomes include preterm parturition and subclinical chorioamnionitis. Inflammatory response induced by GV presents paediatric problems as well. Studies had shown that increased levels of proinflammatory cytokines include TNF-α, IL-1β and IL-6 following fetal inflammatory response syndrome secondary to GV-induced intrauterine infe...
Source: Malaysian Journal of Pathology - December 25, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

Calculation of free and bioavailable vitamin D and its association with bone mineral density in Malaysian women.
We examined this hypothesis in a cohort of Malaysian women. MATERIALS AND METHODS: A cross-sectional study of 77 patients with rheumatoid arthritis (RA) and 29 controls was performed. Serum 25(OH)D was measured using the Roche Cobas E170 immunoassay. Serum vitamin D binding protein (VDBP) was measured using a monoclonal enzyme-linked immunosorbent assay (ELISA). Free/bioavailable 25(OH)D were calculated using both the modified Vermuelen and Bikle formulae. RESULTS: Since there were no significant differences between RA patients and controls for VDBP and 25(OH)D, the dataset was analysed as a whole. Calculated free ...
Source: Malaysian Journal of Pathology - December 25, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

Hepatitis B seroepidemiology and booster vaccination in pre-clinical medical students in a Malaysian university.
This study aimed to investigate seroprevalence of hepatitis B among UKM pre-clinical medical students, born between 1991 and 1995, and had their infant vaccination more than 20 years ago. MATERIALS AND METHODS: A prospective, cross-sectional study involving 352 students, comprising 109 (31.0%) males and 243 (69.0%) females. Blood specimens were tested for anti-HBs, where levels of ≥10 mIU/mL was considered reactive and protective. Students with non-reactive levels were given a 20 μg HBV vaccine booster. Anti-HBs levels were tested six weeks after the first booster dose. Those with anti-HBs 1000 mIU/mL while 255 (72.4%) had anti-HBs
Source: Malaysian Journal of Pathology - December 25, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

The differential roles of caspase family members in mediating PF4-induced breast cancer apoptosis.
This study focused on PF4 effects on caspase-3,-6, -7, -8 and -9 which regulate the apopotosis process in breast cancer. MATERIALS AND METHODS: Breast tumours were induced in forty 21-day-old female Sprague Dawley rats (SDRs) using MNU until tumour size reached 14.5 mm (SD: 0.5 mm). The rats were then divided into two groups: Group 1 (control injected with 0.9% saline; n = 20), and Group 2 (platelet factor 4 (PF4); n = 20). PF4 was administered through focal intralesional injection at 20 μg/lesion dose. Following 5-day treatment, the SDRs were sacrificed. Subsequently, representative sections from the tumour were ob...
Source: Malaysian Journal of Pathology - December 25, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

Cyclooxygenase-2 expression in clear cell renal cell carcinoma.
CONCLUSION: There was no significant association between COX-2 expression with grade, age, sex and ethnicity (p=0.457, p=0.054, p=0.389 and p=0.568 respectively). Strong positivity of COX-2 suggest that COX-2 may play a role in cell proliferation and in carcinogenesis. PMID: 30580362 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - December 25, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

Heterogeneity in studies of p16 in cervical lesions in different Malaysian institutions: Time to consider collaborative study.
CONCLUSION: There was both clinical and methodological heterogeneity making synthesis of studies difficult. It is possible to design a study which would be able to answer all the questions posed by the original authors with internal validity while at the same time increasing sample size. PMID: 30580363 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - December 25, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

Reprogramming human dermal fibroblast into induced pluripotent stem cells using nonintegrative Sendai virus for transduction.
CONCLUSION: NHDF were successfully reprogrammed into iPSC using non-integrating Sendai virus for transduction. PMID: 30580364 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - December 25, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

Intrahepatic cholangiocarcinoma and portal hypertension developing in a patient with multicystic biliary microhamartomas.
We report a case of intrahepatic cholangiocarcinoma and portal hypertension developing in a liver with biliary microhamartomas (von Meyenburg's complex). CASE REPORT: The patient was a 55-year-old man who had a past medical history of diffuse multiple liver abscesses. During follow-up examination, a hypovascular nodule measuring 2.1 cm in diameter was incidentally found in segment 8 of the liver. Surgical resection was performed based on a suspected diagnosis of hepatocellular carcinoma. A gastrofiberscopy examination detected characteristic findings of portal hypertensive gastropathy. During the laparotomy, multiple t...
Source: Malaysian Journal of Pathology - December 25, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

Fatal spontaneous rupture of splenic artery aneurysm in third trimester pregnancy.
CONCLUSION: Knowledge of spontaneous rupture of splenic artery aneurysm in late pregnancy is essential for monitoring maternal and foetal, morbidity and mortality. However, in the eventuality of death a comprehensive forensic autopsy is the only investigation to recognise such calamity and clear clinical confusion. PMID: 30580366 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - December 25, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

Large-cell calcifying Sertoli cell tumour with macrocalcification in partially resected testis of young adult patient.
We present the clinicopathological features of a young adult, with unilateral "stone-like" LCCSCT, without changes in hormonal status and no clinical characteristics of noted genetic disorders. CASE REPORT: A 24-year-old male presented with painless hardening of the right testis. There was no gynaecomastia, and serum levels of human chorionic gonadotropin and α-fetoprotein were normal. Ultrasound depicted hyperechogenic, clearly demarcated intratesticular lesion. Partial orchiectomy was performed. Macroscopically, tumour appeared as almost entirely calcified round mass, measuring 10 mm. Histopathologica...
Source: Malaysian Journal of Pathology - December 25, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

Myofibroblastoma of the breast.
We report a case of myofibroblastoma in an octogenarian male presenting with painless solitary breast lump. Mammography (digital tomosynthesis) and ultrasound showed a well-circumscribed hyperdense mass and hypoechoic, solid, oval mass with peripheral vascularity respectively. Patient underwent wide local excision. DISCUSSION: Diverse characteristics of myofibroblastoma on imaging necessitates histopathological analysis for an accurate diagnosis. Myofibroblastoma are often confused with fibroadenomas due to the benign imaging characteristics and with malignant neoplasia due to their wide morphological spectrum. Surgica...
Source: Malaysian Journal of Pathology - December 25, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

Malignant peripheral nerve sheath tumour with perineurial differentiation and hyaline eosinophilic globules (thanatosomes): A rare tumour.
We reported the clinicoradiological and pathological features of a case of recurrent right arm mass related to the ulnar nerve in a 42-year-old female patient. On pathological examination, the tumour showed dual features of conventional and perineurial MPNST which was proven by positive immunostaining for S-100 and EMA. The pathological diagnosis was MPNST with perineurial differentiation. In addition, a peculiar and rare finding of intracytoplasmic eosinophilic hyaline globules (thanatosomes) within tumour cells is reported. We document a rare tumour with hybrid features between conventional and perineurial MPNSTs. Furthe...
Source: Malaysian Journal of Pathology - December 25, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

Primary follicular lymphoma of the pancreas: A rare tumour mimicking pancreatic carcinoma.
We report a case of a 75-year-old man who presented with symptoms of obstructive jaundice. Ultrasonography and computed tomography (CT) showed an ill-defined lobulated soft tissue lesion at the head/uncinate process of the pancreas measuring 4.5 x 4.9 x 5.8 cm. The patient underwent pancreaticoduodenectomy for suspected pancreatic head/uncinate process carcinoma. Histopathology and immunohistochemical assessment of the pancreatic lesion established the diagnosis of a low-grade follicular lymphoma. DISCUSSION: Clinical and imaging features of primary pancreatic lymphoma may often overlap with pancreatic carcinoma. There...
Source: Malaysian Journal of Pathology - December 25, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

Abstracts of the 5th International Academy of Pathology Malaysian Division, Annual Scientific Meeting 2018: Mediastinal and Breast Pathology.
Authors: Abstract No abstract available. PMID: 30580371 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - December 25, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

Heterogenous expression of ERG oncoprotein in Malaysian men with adenocarcinoma of the prostate.
In this study we investigated expression of ERG in Malaysian men with prostate cancer. METHODS: Tissues were collected from 80 patients with clinically detected prostate cancer and treated with radical prostatectomy. Cases were tested for ERG by immunohistochemistry using the mouse monoclonal antibody EP111. All blocks on 48 cases were tested in order to determine the extent of heterogeneity of ERG expression within individual cases. ERG expression was analysed in relation to patient age, ethnicity and tumour stage and grade. RESULTS: Forty-six percent of cases were ERG positive. There was no significant associatio...
Source: Malaysian Journal of Pathology - September 4, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

CD56 expression in benign and malignant thyroid lesions.
This study aimed to evaluate the diagnostic utility of an immunohistochemical marker, CD56, to distinguish between benign and malignant thyroid lesions. MATERIALS AND METHODS: We retrospectively studied CD56 expression in 54 benign and 54 malignant thyroid lesions using archival formalin fixed paraffin-embedded tissue blocks for the study period from January 2010 to December 2015, diagnosed in a tertiary hospital. RESULTS: CD56 was expressed in 52/54 (96.3%) of benign specimens and only 24/54 (44.4%) of malignant ones. The malignant specimens comprised 31 (57.4%) papillary thyroid carcinomas (PTC), 11 (20.3%) folli...
Source: Malaysian Journal of Pathology - September 4, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

Intraoperative frozen section sentinel lymph node assessment in breast cancer: A tertiary institution experience.
CONCLUSION: Intraoperative frozen section analysis is a safe and reliable method for assessment of sentinel lymph node. Knowledge on limitation of frozen section analysis with diligent evaluation of frozen section specimen will be beneficial in reducing interpretation error. PMID: 30173228 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - September 4, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research

A clinicopathologic study of 173 odontogenic tumours in Northern Peninsular Malaysia (2007-2014).
CONCLUSION: The findings of this study are similar to the other studies in Asia in which the most common tumour encountered is the ameloblastoma, followed by KCOT. The most common signs and symptoms are pain and swelling, while paraesthesia and root resorption are less frequently reported. Such clinical and radiographic features should alert the clinician of a possible odontogenic tumour and though rare, malignant tumours should also be included in the differential diagnoses. PMID: 30173229 [PubMed - in process] (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - September 4, 2018 Category: Pathology Tags: Malays J Pathol Source Type: research