The quantitative impact of read mapping to non-native reference genomes in comparative RNA-Seq studies
In this study, we investigate the practice of using a common reference in transcriptomic analysis. We analyze two multi-strain transcriptomic data sets that were initially presented in the literature as comparisons based on a common reference, but which have available closed genomic sequence for all strains, allowing a detailed examination of the impact of reference choice. We provide a method for identifying regions that are most affected by non-native alignments, leading to false positives in differential expression analysis, and perform an in depth analysis identifying the extent of expression loss. We also simulate se ...
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: Adam Price Source Type: research

The effects of ingestion of hormonal host factors on the longevity and insecticide resistance phenotype of the major malaria vector < i > Anopheles arabiensis < /i > (Diptera: Culicidae)
by Sh üné V. Oliver, Basil D. Brooke Exogenous vertebrate-derived factors circulating in the blood have the capacity to modulate the biology of haematophagous insects. These include insulin, insulin growth factor 1 (IGF) and transforming growth factor β1 (TGFβ). The effects of the consumption of these three proteins were examined o n laboratory strains ofAnopheles arabiensis. SENN, an insecticide susceptible strain and SENN DDT, a resistant strain selected from SENN, were fed with host factor-supplemented sucrose. Adult longevity was measured and insecticide resistance phenotype over time was assesse...
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: Sh üné V. Oliver Source Type: research

Longitudinal increases of brain metabolite levels in 5-10 year old children
by Martha J. Holmes, Frances C. Robertson, Francesca Little, Steven R. Randall, Mark F. Cotton, Andre J. W. van der Kouwe, Barbara Laughton, Ernesta M. Meintjes Longitudinal magnetic resonance imaging (MRI) and diffusion tensor imaging (DTI) studies reveal significant changes in brain structure and structural networks that occur together with cognitive and behavioral maturation in childhood. However, the underlying cellular changes accompanying brain matu ration are less understood. Examining regional age-related changes in metabolite levels provides insight into the physiology of neurodevelopment. Magnetic resonance spec...
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: Martha J. Holmes Source Type: research

Unexpected effects of azole transporter inhibitors on antifungal susceptibility in < i > Candida glabrata < /i > and other pathogenic < i > Candida < /i > species
by Yohsuke Nagayoshi, Taiga Miyazaki, Shintaro Shimamura, Hironobu Nakayama, Asuka Minematsu, Shunsuke Yamauchi, Takahiro Takazono, Shigeki Nakamura, Katsunori Yanagihara, Shigeru Kohno, Hiroshi Mukae, Koichi Izumikawa The pathogenic fungusCandida glabrata is often resistant to azole antifungal agents. Drug efflux through azole transporters, such as Cdr1 and Cdr2, is a key mechanism of azole resistance and these genes are under the control of the transcription factor Pdr1. Recently, the monoamine oxidase A (MAO-A) inhibitor clorgyline was shown to inhibit the azole efflux pumps, leading to increased azole susceptibility i...
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: Yohsuke Nagayoshi Source Type: research

Highly sensitive sandwich immunoassay and immunochromatographic test for the detection of < i > Clostridial < /i > epsilon toxin in complex matrices
by C écile Féraudet-Tarisse, Christelle Mazuet, Serge Pauillac, Maren Krüger, Caroline Lacroux, Michel R. Popoff, Brigitte G. Dorner, Olivier Andréoletti, Marc Plaisance, Hervé Volland, Stéphanie Simon Epsilon toxin is one of the four major toxins ofClostridium perfringens. It is the third most potent clostridial toxin after botulinum and tetanus toxins and is thus considered as a potential biological weapon classified as category B by the Centers for Disease Control and Prevention (CDC). In the case of a bioterrorist attack, there will be a need for a rapid, sensitive and specific ...
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: C écile Féraudet-Tarisse Source Type: research

Mononuclear-macrophages but not neutrophils act as major infiltrating anti-leptospiral phagocytes during leptospirosis
ConclusionsMononuclear-macrophages but not neutrophils represent the main infiltrating and anti-leptospiral phagocytes during leptospirosis. A lower level of phagosome-lysosome fusion may be responsible for the lowerLeptospira-killing ability of human macrophages. (Source: PLoS One)
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: Xu Chen Source Type: research

The dead seed coat functions as a long-term storage for active hydrolytic enzymes
We examined the proposal that dead organs enclosing embryos are unique entities tha t store and release upon hydration active proteins that might increase seed persistence in soil, germination and seedling establishment. Proteome analyses of dead seed coats of Brassicaceae species revealed hundreds of proteins being stored in the seed coat and released upon hydration, many are stre ss-associated proteins such as nucleases, proteases and chitinases. Functional analysis revealed that dead seed coats function as long-term storage for multiple active hydrolytic enzymes (e.g., nucleases) that can persist in active forms for dec...
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: Buzi Raviv Source Type: research

Pediatric autoimmune hepatitis shows a disproportionate decline of regulatory T cells in the liver and of IL-2 in the blood of patients undergoing therapy
ConclusionsIntrahepatic Tregs are rather enriched in untreated pAIH. The disproportional decrease of Tregs during therapy may be caused by a decrease of IL-2 levels. New therapies should, therefore, aim in strengthening intrahepatic immune regulation. (Source: PLoS One)
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: Jana Diestelhorst Source Type: research

Hypoxia tolerance and responses to hypoxic stress during heart and skeletal muscle inflammation in Atlantic salmon ( < i > Salmo salar < /i > )
In conclusion, Atlantic salmon suffering from HSMI have reduced hypoxia tolerance and cardiac performance, which can be improved by preconditioning fish to transient hypoxic stress episodes. (Source: PLoS One)
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: Morten Lund Source Type: research

The association between ethnicity and vaginal microbiota composition in Amsterdam, the Netherlands
ConclusionsThe overall prevalence of having a VMB not dominated by lactobacilli in this population-based cohort of women aged 18 –34 years in Amsterdam was high (38.5%), and women of sub-Saharan African descent were significantly more likely to have a polybacterialG.vaginalis-containing VMB than Dutch women independent of modifiable behaviors. (Source: PLoS One)
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: Hanneke Borgdorff Source Type: research

Occupation with grain crops is associated with lower type 1 diabetes incidence: Registry-based case-control study
by Martin Haupt-Jorgensen, Erik Nielsen, K åre Engkilde, Mia Lerche, Jesper Larsen, Karsten Buschard Intranasal administration of gliadin prevents autoimmune diabetes in non-obese diabetic mice. The current study was designed to investigate if bakers are intranasally exposed to gluten during work and whether occupation as baker is inversely associated with type 1 diabetes. Gliadin was measured in nasal swabs from eight bakers and butchers. The odds ratio of type 1 diabetes in selected profession groups was analysed in a registry-based case-control study with data from 1980 to 2010 derived from Statistics Denmark. Th...
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: Martin Haupt-Jorgensen Source Type: research

Age dependent differences in the kinetics of γδ T cells after influenza vaccination
In conclusion, aging induces alterations of the γδ T cell response that might have negative implications for vaccination efficacy. (Source: PLoS One)
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: Ulrik Stervbo Source Type: research

Molecular evolution of versatile derivatives from a GFP-like protein in the marine copepod < i > Chiridius poppei < /i >
In this study, which was aimed at developing YGFP mutants with increased Stokes shifts, we conducted stepwise molecular evolution experiments on YGFP by screening random mutations at three key amino acids, based on their fluorescent characteristics and structural stabilities, followed by optimization of their fluorescence output by DNA shuffling of the entire coding sequence. We successfully identified an eYGFPuv that had an excitation maximum in UV wavelengths and a 24-fold increase in fluorescence intensity compared to the previously reported YGFP mutant (H52D). In addition, eYGFPuv exhibited almost 9-fold higher fluores...
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: Akihisa Shimizu Source Type: research

Turns with multiple and single head cast mediate < i > Drosophila < /i > larval light avoidance
by Weiqiao Zhao, Caixia Gong, Zhenhuan Ouyang, Pengfei Wang, Jie Wang, Peipei Zhou, Nenggan Zheng, Zhefeng GongDrosophila larvae exhibit klinotaxis when placed in a gradient of temperature, chemicals, or light. The larva samples environmental stimuli by casting its head from side to side. By comparing the results of two consecutive samples, it decides the direction of movement, appearing as a turn proceeded by one or more head casts. Here by analyzing larval behavior in a light-spot-based phototaxis assay, we showed that, in addition to turns with a single cast (1-cast), turns with multiple head casts (n-cast) helped to im...
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: Weiqiao Zhao Source Type: research

Gallic acid/hydroxypropyl- β-cyclodextrin complex: Improving solubility for application on < i > in vitro/ in vivo Candida albicans < /i > biofilms
This study encourages the development of new antifungal agents. (Source: PLoS One)
Source: PLoS One - July 11, 2017 Category: Biomedical Science Authors: Guilherme Rodrigues Teodoro Source Type: research

Cancer-selective, single agent chemoradiosensitising gold nanoparticles
by Sophie Grellet, Konstantina Tzelepi, Meike Roskamp, Phil Williams, Aquila Sharif, Richard Slade-Carter, Peter Goldie, Nicky Whilde, Ma łgorzata A. Śmiałek, Nigel J. Mason, Jon P. Golding Two nanometre gold nanoparticles (AuNPs), bearing sugar moieties and/or thiol-polyethylene glycol-amine (PEG-amine), were synthesised and evaluated for theirin vitro toxicity and ability to radiosensitise cells with 220 kV and 6 MV X-rays, using four cell lines representing normal and cancerous skin and breast tissues. Acute 3 h exposure of cells to AuNPs, bearing PEG-amine only or a 50:50 ratio of alpha-galactose derivative and PEG...
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: Sophie Grellet Source Type: research

Predictors and long-term health outcomes of eating disorders
by Katie M. O ’Brien, Denis R. Whelan, Dale P. Sandler, Janet E. Hall, Clarice R. Weinberg Anorexia and bulimia nervosa may have long-term effects on overall and reproductive health. We studied predictors of self-reported eating disorders and associations with later health events. We estimated odds ratios (ORs) for these associations in 47,759 participants from the Sister Study. Two per cent (n = 967) of participants reported a history of an eating disorder. Risk factors included being non-Hispanic white, having well-educated parents, recent birth cohort (OR = 2.16, 95% confidence interval [CI]: 2.01–2.32 per ...
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: Katie M. O ’Brien Source Type: research

Characterization of the molecular chaperone ClpB from the pathogenic spirochaete < i > Leptospira interrogans < /i >
by Joanna Krajewska, Anna Modrak-W ójcik, Zbigniew J. Arent, Daniel Więckowski, Michal Zolkiewski, Agnieszka Bzowska, Sabina Kędzierska-MieszkowskaLeptospira interrogans is a spirochaete responsible for leptospirosis in mammals. The molecular mechanisms of theLeptospira virulence remain mostly unknown. Recently, it has been demonstrated that an AAA+ chaperone ClpB (a member of the Hsp100 family) fromL.interrogans (ClpBLi) is not only essential for survival ofLeptospira under the thermal and oxidative stresses, but also during infection of a host. The aim of this study was to provide further insight into the role o...
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: Joanna Krajewska Source Type: research

Effects of taurine on resting-state fMRI activity in spontaneously hypertensive rats
by Vincent Chin-Hung Chen, Tsai-Ching Hsu, Li-Jeng Chen, Hong-Chun Chou, Jun-Cheng Weng, Bor-Show Tzang Attention deficit hyperactivity disorder (ADHD) is a global behavior illness among children and adults. To investigate the effects of taurine on resting-state fMRI activity in ADHD, a spontaneously hypertensive rat (SHR) animal model was adopted. Significantly decreased serum C-reactive protein (C RP) was detected in rats of Wistar Kyoto (WKY) high-taurine group and significantly decreased interleukin (IL)-1β and CRP were detected in rats of SHR low-taurine and high-taurine groups. Moreover, significantly higher ho...
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: Vincent Chin-Hung Chen Source Type: research

Correction: Central Presynaptic Terminals Are Enriched in ATP but the Majority Lack Mitochondria
by Vrushali Chavan, Jeffery Willis, Sidney K. Walker, Helen R. Clark, Xinran Liu, Michael A. Fox, Sarika Srivastava, Konark Mukherjee (Source: PLoS One)
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: Vrushali Chavan Source Type: research

Acute kidney injury after cardiac surgery is associated with mid-term but not long-term mortality: A cohort-based study
by Alejandro Ferreiro, Ra úl Lombardi Acute kidney injury (AKI) in cardiac surgery is associated with complications, early and late mortality and increased health care expenditures. The overall dynamic comorbidity-adjusted contributions of an episode of AKI on mortality during long-term follow-up have not been fully explored. A longit udinal cohort of 7075 adult patients admitted for cardiac surgery were enrolled in the study. Follow-up data were obtained through telephonic survey after 1, 5, 10, and 15 years or from the National Mortality Registry. All-cause mortality was assessed at five time intervals: I) 30 day...
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: Alejandro Ferreiro Source Type: research

A high seroprevalence of antibodies to pertussis toxin among Japanese adults: Qualitative and quantitative analyses
by Takumi Moriuchi, Nao Otsuka, Yukihiro Hiramatsu, Keigo Shibayama, Kazunari Kamachi In 2013, national serosurveillance detected a high seroprevalence of antibodies to pertussis toxin (PT) fromBordetella pertussis among Japanese adults. Thus, we aimed to determine the cause(s) of this high seroprevalence, and analyzed the titers of antibodies to PT and filamentous hemagglutinin (FHA) among adults (35 –44 years old), young children (4–7 years old), and older children (10–14 years old). Our quantitative analyses revealed that adults had higher seroprevalences of anti-PT IgG and PT-neutralizing antibodies,...
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: Takumi Moriuchi Source Type: research

Forward flexion of trunk in Parkinson's disease patients is affected by subjective vertical position
by Kyohei Mikami, Makoto Shiraishi, Tsubasa Kawasaki, Tsutomu Kamo PurposeNo method has been established to evaluate the dissociation between subjective and objective vertical positions with respect to the self-awareness of postural deformity in patients with Parkinson ’s disease (PD). The purpose of this study was to demonstrate, from the relationship between an assessment of the dissociation of subjective and objective vertical positions of PD patients and an assessment based on established PD clinical evaluation scales, that the dissociation regarding vertica l position is a factor in the severity of the forward f...
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: Kyohei Mikami Source Type: research

Reduction of diabetes-related distress predicts improved depressive symptoms: A secondary analysis of the DIAMOS study
by Andr é Reimer, Andreas Schmitt, Dominic Ehrmann, Bernhard Kulzer, Norbert Hermanns ObjectiveDepressive symptoms in people with diabetes are associated with increased risk of adverse outcomes. Although successful psychosocial treatment options are available, little is known about factors that facilitate treatment response for depression in diabetes. This prospective study aims to examine the impact of known risk factors on improvement of depressive symptoms with a special interest in the role of diabetes-related distress. Methods181 people with diabetes participated in a randomized controlled trial. Diabetes-relat...
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: Andr é Reimer Source Type: research

Correction: Genotypic and phenotypic diversity of < i > Lactobacillus rhamnosus < /i > clinical isolates, their comparison with strain GG and their recognition by complement system
by Eija Nissil ä, François P. Douillard, Jarmo Ritari, Lars Paulin, Hanna M. Järvinen, Pia Rasinkangas, Karita Haapasalo, Seppo Meri, Hanna Jarva, Willem M. de Vos (Source: PLoS One)
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: Eija Nissil ä Source Type: research

Correction: A selection study on a laboratory-designed population of salmon lice ( < i > Lepeophtheirus salmonis < /i > ) using organophosphate and pyrethroid pesticides
by The PLOS ONE Staff (Source: PLoS One)
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: The PLOS ONE Staff Source Type: research

Correction: Production of IL-8, VEGF and Elastase by Circulating and Intraplaque Neutrophils in Patients with Carotid Atherosclerosis
by Franca Marino, Matteo Tozzi, Laura Schembri, Stefania Ferraro, Antonino Tarallo, Angela Scanzano, Massimiliano Legnaro, Patrizio Castelli, Marco Cosentino (Source: PLoS One)
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: Franca Marino Source Type: research

Correction: Measurement of macular structure-function relationships using spectral domain-optical coherence tomography (SD-OCT) and pattern electroretinograms (PERG)
by Keunheung Park, Jinmi Kim, Jiwoong Lee (Source: PLoS One)
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: Keunheung Park Source Type: research

Correction: < i > E6 < /i > and < i > E7 < /i > Gene Polymorphisms in Human Papillomavirus Types-58 and 33 Identified in Southwest China
by Zuyi Chen, Yaling Jing, Qiang Wen, Xianping Ding, Tao Wang, Xuemei Mu, Yuwei Chenzhang, Man Cao (Source: PLoS One)
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: Zuyi Chen Source Type: research

Correction: Adipocyte arrestin domain-containing 3 protein (Arrdc3) regulates uncoupling protein 1 (Ucp1) expression in white adipose independently of canonical changes in β-adrenergic receptor signaling
by Shannon H. Carroll, Ellen Zhang, Bing F. Wang, Katherine B. LeClair, Arifeen Rahman, David E. Cohen, Jorge Plutzky, Parth Patwari, Richard T. Lee (Source: PLoS One)
Source: PLoS One - July 10, 2017 Category: Biomedical Science Authors: Shannon H. Carroll Source Type: research

Plasminogen Tochigi mice exhibit phenotypes similar to wild-type mice under experimental thrombotic conditions
by Yuko Tashima, Fumiaki Banno, Toshiyuki Kita, Yasuyuki Matsuda, Hiroji Yanamoto, Toshiyuki Miyata Plasminogen (Plg) is a precursor of plasmin that degrades fibrin. A race-specific A620T mutation in Plg, also known as Plg-Tochigi, originally identified in a patient with recurrent venous thromboembolism, causes dysplasminogenemia with reduced plasmin activity. The Plg-A620T mutation is present i n 3–4% of individuals in East Asian populations, and as many as 50,000 Japanese are estimated to be homozygous for the mutant 620T allele. In the present study, to understand the changes of thrombotic phenotypes in individua...
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: Yuko Tashima Source Type: research

A preliminary approach to quantifying the overall environmental risks posed by development projects during environmental impact assessment
by Sam Nicol, Iadine Chad ès Environmental impact assessment (EIA) is used globally to manage the impacts of development projects on the environment, so there is an imperative to demonstrate that it can effectively identify risky projects. However, despite the widespread use of quantitative predictive risk models in areas suc h as toxicology, ecosystem modelling and water quality, the use of predictive risk tools to assess the overall expected environmental impacts of major construction and development proposals is comparatively rare. A risk-based approach has many potential advantages, including improved predictio...
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: Sam Nicol Source Type: research

Prognostic score in patients with recurrent or metastatic carcinoma of the head and neck treated with cetuximab and chemotherapy
by Teresa Magnes, Thomas Melchardt, Lukas Weiss, Christof Mittermair, Daniel Neureiter, Eckhard Klieser, Simon Gampenrieder, Gerhard Moser, Alexander Gaggl, Richard Greil, Alexander Egle Despite modern treatment approaches, survival of patients with recurrent or metastatic squamous cell carcinoma of the head and neck (SCCHN) remains low and it is difficult to identify patients who derive optimal benefit from treatment. We therefore analyzed which commonly available laboratory and clinical parameters may help improve the prognostication in this patient group. This retrospective monocenter analysis includes 128 patients wit...
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: Teresa Magnes Source Type: research

< i > In-vitro < /i > release pharmacokinetics of amikacin, teicoplanin and polyhexanide in a platelet rich fibrin —layer (PRF)—a laboratory evaluation of a modern, autologous wound treatment
ConclusionsOur Results show that a weekly dressing regimen may be justified in wounds treated with PRF plus amikacin or teicoplanin, since bacteria will be eradicated over a considerable period of time after a single application of PRF. (Source: PLoS One)
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: Daniela Knafl Source Type: research

Obesity/Type II diabetes alters macrophage polarization resulting in a fibrotic tendon healing response
by Jessica E. Ackerman, Michael B. Geary, Caitlin A. Orner, Fatima Bawany, Alayna E. Loiselle Type II Diabetes (T2DM) dramatically impairs the tendon healing response, resulting in decreased collagen organization and mechanics relative to non-diabetic tendons. Despite this burden, there remains a paucity of information regarding the mechanisms that govern impaired healing of diabetic tendo ns. Mice were placed on either a high fat diet (T2DM) or low fat diet (lean) and underwent flexor tendon transection and repair surgery. Healing was assessed via mechanical testing, histology and changes in gene expression associated wi...
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: Jessica E. Ackerman Source Type: research

Correction: Recessive coding and regulatory mutations in < i > FBLIM1 < /i > underlie the pathogenesis of chronic recurrent multifocal osteomyelitis (CRMO)
by Allison J. Cox, Benjamin W. Darbro, Ronald M. Laxer, Gabriel Velez, Xinyu Bing, Alexis L. Finer, Albert Erives, Vinit B. Mahajan, Alexander G. Bassuk, Polly J. Ferguson (Source: PLoS One)
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: Allison J. Cox Source Type: research

Correction: Heart rate variability during hemodialysis is an indicator for long-term vascular access survival in uremic patients
by Ya-Ting Huang, Yu-Ming Chang, I-Ling Chen, Chuan-Lan Yang, Show-Chin Leu, Hung-Li Su, Jsun-Liang Kao, Shih-Ching Tsai, Rong-Na Jhen, Woung-Ru Tang, Chih-Chung Shiao, On behalf of SMHRG (Saint Mary ’s Hospital Research Group on Geriatrics) (Source: PLoS One)
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: Ya-Ting Huang Source Type: research

Correction: Prevalence and extent of heteroresistance by next generation sequencing of multidrug-resistant tuberculosis
by Darwin J. Operario, Alexander F. Koeppel, Stephen D. Turner, Yongde Bao, Suporn Pholwat, Sayera Banu, Suporn Foongladda, Stellah Mpagama, Jean Gratz, Oleg Ogarkov, Svetlana Zhadova, Scott K. Heysell, Eric R. Houpt (Source: PLoS One)
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: Darwin J. Operario Source Type: research

Correction: Immuno-related polymorphisms and cervical cancer risk: The IARC multicentric case-control study
by James McKay, Vanessa Tenet, Silvia Franceschi, Am élie Chabrier, Tarik Gheit, Valérie Gaborieau, Sandrine McKay-Chopin, Patrice H. Avogbe, Massimo Tommasino, Michelle Ainouze, Uzma Hasan, Salvatore Vaccarella (Source: PLoS One)
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: James McKay Source Type: research

Correction: Unhealthy habits persist: The ongoing presence of modifiable risk factors for disease in women
by Cassandra Szoeke, Christa Dang, Philippe Lehert, Martha Hickey, Meg E. Morris, Lorraine Dennerstein, Stephen Campbell (Source: PLoS One)
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: Cassandra Szoeke Source Type: research

Correction: Functional restoration of the esophagus after peroral endoscopic myotomy for achalasia
by Cheal Wung Huh, Young Hoon Youn, Hyunsoo Chung, Yong Chan Lee, Hyojin Park (Source: PLoS One)
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: Cheal Wung Huh Source Type: research

Correction: Real-time cell toxicity profiling of Tox21 10K compounds reveals cytotoxicity dependent toxicity pathway linkage
by Jui-Hua Hsieh, Ruili Huang, Ja-An Lin, Alexander Sedykh, Jinghua Zhao, Raymond R. Tice, Richard S. Paules, Menghang Xia, Scott S. Auerbach (Source: PLoS One)
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: Jui-Hua Hsieh Source Type: research

Correction: T1R3 homomeric sweet taste receptor regulates adipogenesis through G αs-mediated microtubules disassembly and Rho activation in 3T3-L1 cells
by Yosuke Masubuchi, Yuko Nakagawa, Johan Medina, Masahiro Nagasawa, Itaru Kojima, Mark M. Rasenick, Takeshi Inagaki, Hiroshi Shibata (Source: PLoS One)
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: Yosuke Masubuchi Source Type: research

Correction: Physiological responses and external validity of a new setting for taekwondo combat simulation
by Matheus Hausen, Pedro Paulo Soares, Marcus Paulo Ara újo, Flávia Porto, Emerson Franchini, Craig Alan Bridge, Jonas Gurgel (Source: PLoS One)
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: Matheus Hausen Source Type: research

Correction: Hepatitis C Virus Core Protein Down-Regulates p21 < sup > Waf1/Cip1 < /sup > and Inhibits Curcumin-Induced Apoptosis through MicroRNA-345 Targeting in Human Hepatoma Cells
by Tzu-Yue Shiu, Shih-Ming Huang, Yu-Lueng Shih, Heng-Cheng Chu, Wei-Kuo Chang, Tsai-Yuan Hsieh (Source: PLoS One)
Source: PLoS One - July 7, 2017 Category: Biomedical Science Authors: Tzu-Yue Shiu Source Type: research

Visceral fat reference values derived from healthy European men and women aged 20-30 years using GE Healthcare dual-energy x-ray absorptiometry
In conclusion, we provide reference values for VAT obtained from healthy adults using the GE Healthcare DXA. These values may be useful in the diagnosis of visceral obesity, for identifying subjects with high obesity-related risks, in epidemiologic al studies, as a target for therapies, and in physically trained individuals. In both genders, VAT was associated with traditional cardiometabolic risk factors, particularly hypertriglyceridemia. (Source: PLoS One)
Source: PLoS One - July 6, 2017 Category: Biomedical Science Authors: Tomasz Miazgowski Source Type: research

Demonstration of disinfection procedure for the development of accurate blood glucose meters in accordance with ISO 15197:2013
In this study, an effective disinfection procedure, conducting wiping 10 times to assure a one minute contact time of the disinfectant on contaminated surface, was first demonstrated using test samples of the meter housing materia ls, including acrylonitrile butadiene styrene (ABS), polymethyl methacrylate (PMMA), and polycarbonate (PC), in accordance with ISO 15197:2013. After bench studies comprising 10,000 disinfection cycles, the elemental compositions of the disinfected ABS, PMMA, and PC samples were almost the same as i n the original samples, as indicated by electron spectroscopy for chemical analysis. Subsequently,...
Source: PLoS One - July 6, 2017 Category: Biomedical Science Authors: Shu-Ping Lin Source Type: research

Cardiovascular risk associated with high sodium-containing drugs: A systematic review
ConclusionDespite numerous study limitations, this systematic review suggests three potential synergistic risk factors for cardiovascular complications after exposure to HSCD: a high sodium intake ( ≥ 1,500 mg/day), a long duration of exposure, and the presence of comorbidities. Further studies are required to characterize this iatrogenic risk. Trial registrationPROSPERO CRD42016047086. (Source: PLoS One)
Source: PLoS One - July 6, 2017 Category: Biomedical Science Authors: Germain Perrin Source Type: research

Assessment of some cultural experimental methods to study the effects of antibiotics on microbial activities in a soil: An incubation study
This study was conducted based on the completely randomized design to the measure microbial biomas s carbon, cumulative respiration and iron (III) reduction bioassays. Concentrations of OTC and SMX including 0, 1, 10, 25, 50, and 100 mg/kg were spiked in triplicate to a sandy loam soil and incubated for 21 days at 25°C. Results showed that the effects of OTC and SMX antibiotics on cumulative res piration and microbial biomass carbon were different. SMX antibiotic significantly affected soil microbial biomass carbon and cumulative respiration at different treatments compared to control with increasing incubation time. O...
Source: PLoS One - July 6, 2017 Category: Biomedical Science Authors: Ali Molaei Source Type: research

Morgagnian cataract resulting from a naturally occurring nonsense mutation elucidates a role of CPAMD8 in mammalian lens development
by Anne K. Hollmann, Insa Dammann, Wiebke M. Wemheuer, Wilhelm E. Wemheuer, Almuth Chilla, Andrea Tipold, Walter J. Schulz-Schaeffer, Julia Beck, Ekkehard Sch ütz, Bertram Brenig To investigate the genetic basis of hereditary lens opacities we analyzed 31 cases of bilateral congenital cataract in Red Holstein Friesian cattle. A genome-wide association study revealed a significant association on bovine chromosome 7 at positions 6,166,179 and 12,429,691. Whole genome re-sequ encing of one case and four relatives showed a nonsense mutation (g.5995966C>T) in the PZP-like, alpha-2-macroglobulin domain containing 8 (CPA...
Source: PLoS One - July 6, 2017 Category: Biomedical Science Authors: Anne K. Hollmann Source Type: research