Multiparameter immunoprofiling for the diagnosis and differentiation of progressive versus nonprogressive nontuberculous mycobacterial lung disease –A pilot study
by Paige K. Marty, Balaji Pathakumari, Thomas M. Cox, Virginia P. Van Keulen, Courtney L. Erskine, Maleeha Shah, Mounika Vadiyala, Pedro Arias-Sanchez, Snigdha Karnakoti, Kelly M. Pennington, Elitza S. Theel, Cecilia S. Lindestam Arlehamn, Tobias Peikert, Patricio Escalante
Clinical prediction of nontuberculous mycobacteria lung disease (NTM-LD) progression remains challenging. We aimed to evaluate antigen-specific immunoprofiling utilizing flow cytometry (FC) of activation-induced markers (AIM) and IFN-γ enzyme-linked immune absorbent spot assay (ELISpot) accuratel y identifies patients with NTM-LD, and differentiate th...
Source: PLoS One - April 19, 2024 Category: Biomedical Science Authors: Paige K. Marty Source Type: research
Effects of (2 < i > R < /i > ,6 < i > R < /i > )-hydroxynorketamine in assays of acute pain-stimulated and pain-depressed behaviors in mice
by Todd M. Hillhouse, Kaitlyn J. Partridge, Patrick I. Garrett, Sarah C. Honeycutt, Joseph H. Porter
Ketamine has been shown to produce analgesia in various acute and chronic pain states; however, abuse liability concerns have limited its utility. The ketamine metabolite (2R,6R)-hydroxynorketamine (HNK) has been shown to produce antidepressant-like effects similar to ketamine without abuse liability concerns. (2R,6R)-HNK produces sustained analgesia in models of chronic pain, but has yet to be evaluated in models of acute pain. The present study evaluated the efficacy of acute (2R,6R)-HNK administration (one injection) in...
Source: PLoS One - April 19, 2024 Category: Biomedical Science Authors: Todd M. Hillhouse Source Type: research
< i > Spilanthes filicaulis < /i > (Schumach. & Thonn.) C.D. Adams leaves protects against streptozotocin-induced diabetic nephropathy
ConclusionTherefore, our results indicate that MESFL alleviates the development of diabetic nephropathy via suppression of the cAMP/PKA/CREB/cFOS pathways. (Source: PLoS One)
Source: PLoS One - April 19, 2024 Category: Biomedical Science Authors: Oluwafemi Adeleke Ojo Source Type: research
Changes in parenting behavior in the time of COVID —19: A mixed method approach
This study was designed to explore mothers’ perceptions about changes in parenting behavior in the middle of the pandemic COVID 19 period. Based on the convergent mixed-method design and Parental Stress model, we illustrated these changes by taking into account the impact of the pandemic perceiv ed by mothers and the resources they had available. Research on parenting changes was important in the Romanian context because, in that challenging period, there were no regulations to safeguard parents, especially single parents as mothers. Mothers experienced increased levels of stress, some of t hem having to leave their jobs...
Source: PLoS One - April 19, 2024 Category: Biomedical Science Authors: Luiza Mesesan-Schmitz Source Type: research
Test-retest reliability of Latin American Group for Maturity (GDLAM) protocol in older women
This study evaluated the test-retest reliability of GDLAM and GDLAM autonomy index (GI) in OW. Thirty-nine OW (71.2 ± 6.50 years) participated in the study. A repeated measures design was used to compare the interday test-retest reliability of the five GDLAM tests (seconds) and the GI (points). The five tests represen t activities of daily living, such as dressing or wandering around the house, while the GI provides a weighting of the results of the five tests. The analysis consisted of the intraclass correlation coefficient (ICC), standard error of measurement (SEM), and coefficient of variation (CV). A CV ≤ 1 0% and a...
Source: PLoS One - April 19, 2024 Category: Biomedical Science Authors: Álvaro Huerta Ojeda Source Type: research
Self-administration medication errors at home and its predictors among illiterate and low-literate community-dwelling older adults with polypharmacy: A negative binomial hierarchical regression
This study aimed to identify the extent of medication self-administration errors (MSEs) and the contributing factors among illiterate and low-literate community-dwelling older adults with polypharmacy. MethodThe present cross-sectional study was conducted among people aged 60 and above. The data were collected using the sociodemographic, clinical, and Belief about Medicines Questionnaires (BMQ). To determine the extent of MSE, a medication error checklist was used. The negative binomial hierarchical regression model in the five blocks was performed. ResultsThe final sample size was 276 people. The frequency of MSEs in the ...
Source: PLoS One - April 19, 2024 Category: Biomedical Science Authors: Nafiseh Ghassab-Abdollahi Source Type: research
Tobacco price elasticity by socioeconomic characteristics in Ecuador
This article aims to estimate the price and income elasticity of cigarettes for different population groups in Ecuador. The National Survey of Urban and Rural Household I ncome and Expenditures (ENIGHUR) 2011–2012 was used, which has information on household cigarette consumption and its sociodemographic characteristics. Deaton’s Almost Ideal Demand System, which decouples the effect of quality on the price of the good, was applied. The elasticities were calculat ed for several groups: urban/rural, income levels (tertiles), education level, sex and age ranges of the household head, and frequency of cigarette purchases ...
Source: PLoS One - April 19, 2024 Category: Biomedical Science Authors: Ana Cristina Mena Source Type: research
Knowledge and perception about the welfare and mistreatment of dogs in Brazil
This study sought to a ppraise the factors influencing the Brazilian population’s understanding of dog welfare and mistreatment. The survey questions were adapted from the Animal Welfare Examination Protocol, utilized by veterinarians to evaluate suspected passive abuse cases in dogs. Out of 1377 responses, 1353 were va lid and analyzed. Among the 19 assessed indicators, 15 demonstrated an adequate response rate surpassing 90% of all 1353 responses. However, for three questions related to comfort, a smaller yet notable percentage of responses were only minimally adequate. Moreover, in one question within the comfor t ass...
Source: PLoS One - April 19, 2024 Category: Biomedical Science Authors: Gabriela Ferreira Siano Source Type: research
A bilateral filtering-based image enhancement for Alzheimer disease classification using CNN
This study aims to develop an optimally performing convolutional neural network to classify Alzheimer’s disease into mild cognitive impairment, normal controls, or Alzheimer’s disease classes using a magnetic resonance imaging dataset. To achieve this, we focused the study on addressing the ch allenge of image noise, which impacts the performance of deep learning models. The study introduced a scheme for enhancing images to improve the quality of the datasets. Specifically, an image enhancement algorithm based on histogram equalization and bilateral filtering techniques was deployed to re duce noise and enhance the qua...
Source: PLoS One - April 19, 2024 Category: Biomedical Science Authors: Nicodemus Songose Awarayi Source Type: research
Relying on the French territorial offer of thermal spa therapies to build a care pathway for long COVID-19 patients
ConclusionBased on the example of the French spa offer, this study makes it possible to place the care pathways of long COVID-19 patients in a wider area (at least national), rather than limiting them to clinical and local management in a hospital setting. The identification and operationalization of two geographical criteria for integrating a type of treatment such as a spa cure into a care pathway contributes to a finer conceptualization of the construction of healthcare pathways. (Source: PLoS One)
Source: PLoS One - April 19, 2024 Category: Biomedical Science Authors: Milhan Chaze Source Type: research
Xinnaotongluo liquid protects H9c2 cells from H/R-induced damage by regulating MDM2/STEAP3
by Jiankun Cui, Qinwen Wang, Minghao Li
Xinnaotongluo liquid has been used to improve the clinical symptoms of patients with myocardial infarction. However, the molecular mechanism of Xinnaotongluo liquid is not completely understood. H9c2 cells exposed to hypoxia/reoxygenation (H/R) was used to simulate damage to cardiomyocytes in myoc ardial infarction in vitro. The biological indicators of H9c2 cells were measured by cell counting kit-8, enzyme linked immunoabsorbent assay, and western blot assay. In H/R-induced H9c2 cells, a markedly reduced murine double minute 2 (MDM2) was observed. However, the addition of Xinnaoto...
Source: PLoS One - April 19, 2024 Category: Biomedical Science Authors: Jiankun Cui Source Type: research
From code to care: Clinician and researcher perspectives on an optimal therapeutic web portal for acute myeloid leukemia
ConclusionsThis research adds to existing calls for digital platforms for researchers and clinicians to supplement extant modes of communication to streamline research and its dissemination, advance precision medicine, and ultimately improve patient prognosis and care. Findings are applicable to therapeutic web portals more generally, particularly in genomic and translational medicine, and will be of interest to portal end-users, developers, researchers, and policymakers. (Source: PLoS One)
Source: PLoS One - April 18, 2024 Category: Biomedical Science Authors: Terese Knoppers Source Type: research
Birth preparedness and complication readiness among recently delivered women in Hargeisa town, Somaliland: A community-based cross-sectional study
ConclusionThe study identified that the BPCR level was very low compared to many other studies. Such a low BPCR level may have a negative impact on maternal health and lives, hindering interventions conducted to reduce maternal mortality rates. All concerned bodies should consider the importance of awareness creation regarding BPCR in their core interventions. (Source: PLoS One)
Source: PLoS One - April 18, 2024 Category: Biomedical Science Authors: Abdeta Muktar Ahmed Source Type: research
Incidence rates of the most common canine tumors based on data from the Swiss Canine Cancer Registry (2008 to 2020)
In conclusion, the calculated IRs and IRRs revealed previously unknown predisposing factors, including novel breed-specific suscepti bilities. The results may have implications for cancer screening, diagnostic work-up, breeding management and oncologic and translational research. (Source: PLoS One)
Source: PLoS One - April 18, 2024 Category: Biomedical Science Authors: Elena Sophie Dhein Source Type: research
Feasibility and preliminary effects of a mindfulness-based physical exercise (MBPE) program for community-dwelling older people with sarcopenia: A protocol for a parallel, two-armed pilot randomised controlled trial
ConclusionThe findings of this study will be able to provide evidence for the health professionals in adopting MBPE as a supportive intervention for the older adults with sarcopenia and the groundworks for the researchers in developing non-pharmacological intervention for older adults. The positive effects could facilitate healthy ageing and relief the burden of the medical system, especially in the countries facing the ageing population. Trial registration numberNCT05982067; ClinicalTrials.gov. (Source: PLoS One)
Source: PLoS One - April 18, 2024 Category: Biomedical Science Authors: Meng-Li Li Source Type: research
