Laboratory diagnosis of COVID-19: current status and challenges
Iran J Microbiol. 2021 Feb;13(1):1-7. doi: 10.18502/ijm.v13i1.5485.ABSTRACTThe magnitude and pace of global affliction caused by Coronavirus Disease-19 (COVID-19) is unprecedented in the recent past. From starting in a busy seafood market in the Chinese city of Wuhan, the virus has spread across the globe in less than a year, infecting over 76 million people and causing death of close to 1.7 million individuals worldwide. As no specific antiviral treatment is currently available, the major strategy in containing the pandemic is focused on early diagnosis and prompt isolation of the infected individuals. Several diagnostic ...
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Bijina J Mathew Ashish Kumar Vyas Prashant Khare Sudheer Gupta Ram Kumar Nema Shashwati Nema Sudipti Gupta Shivendra K Chaurasiya Debasis Biswas Anirudh K Singh Source Type: research

Differential alteration trend of white blood cells (WBCs) and monocytes count in severe and non-severe COVID-19 patients within a 7-day follow-up
CONCLUSION: Alteration of monocytes either on admission or within hospitalization would not provide valuable data about the prediction of COVID-19 prognosis. Although the rapidly evolving nature of COVID-19 is the major limitation of the present study, further investigations in the field of laboratory biomarkers will pave the way to manage patients with severe disease better.PMID:33889357 | PMC:PMC8043835 | DOI:10.18502/ijm.v13i1.5486 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Ali Pirsalehi Sina Salari Ahmadreza Baghestani Ghazal Sanadgol Dorsa Shirini Maryam Moghbel Baerz Saeed Abdi Mohammad Esmaeil Akbari Davood Bashash Source Type: research

Transcriptome based analysis of apoptosis genes in chickens co-infected with avian infectious bronchitis virus and pathogenic < em > Escherichia coli < /em >
CONCLUSION: All of the apoptotic genes in our study were up-regulated. Among these genes, the more fold change value was for TRADD and BCL2A1 genes, and the less fold change value was for MAP3K14, NFKB1, PIK3CB, and ITPR2 genes.PMID:33889358 | PMC:PMC8043830 | DOI:10.18502/ijm.v13i1.5487 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Shabnam Hashemi Seyed Masoud Hosseini Arash Ghalyanchilangeroudi Nariman Sheikhi Source Type: research

Clinical characteristics and molecular detection of < em > Bordetella pertussis < /em > in hospitalized children with a clinical diagnosis of whooping cough in Peru
CONCLUSION: In conclusion, in the studied population there is a high rate of PCR+ cases for B. pertussis. Laboratory values may show leukopenia or lymphopenia in patients with pertussis. It is necessary to use appropriate laboratory diagnostic tests in all infants with respiratory symptoms for B. pertussis. Since, the clinical diagnosis overestimates the diagnosis of pertussis.PMID:33889359 | PMC:PMC8043828 | DOI:10.18502/ijm.v13i1.5488 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Juana Del Valle-Mendoza Cristina Del Valle-Vargas Ronald Aquino-Ortega Luis J Del Valle Erico Cieza-Mora Wilmer Silva-Caso Jorge Baz án-Mayra Victor Zavaleta-Gavidia Miguel Angel Aguilar-Luis Hern án Cornejo-Pacherres Johanna Martins-Luna Angela Cornejo Source Type: research

Comparison of PCR and phenotypic methods for the detection of methicillin resistant < em > Staphylococcus aureus < /em >
CONCLUSION: Based on the findings in our study, isolates which show cefoxitin zone diameter
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Anitha Madhavan Arun Sachu Anukumar Balakrishnan Anu Vasudevan Sobha Balakrishnan Jayalakshmi Vasudevapanicker Source Type: research

Effect of azithromycin and phenylalanine-arginine beta-naphthylamide on quorum sensing and virulence factors in clinical isolates of < em > Pseudomonas aeruginosa < /em >
CONCLUSION: Anti-QS agents like AZM and EPI (PAβN) are useful therapeutic options for P. aeruginosa due to its inhibitory effect on QS-dependent virulence factors production without selective pressure on bacteria growth, so resistance to these agents is less likely to develop.PMID:33889361 | PMC:PMC8043820 | DOI:10.18502/ijm.v13i1.5491 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Amel Elsheredy Ingy El-Soudany Eglal Elsherbini Dalia Metwally Abeer Ghazal Source Type: research

Antimicrobial susceptibility pattern of carbapenemase-producing Gram-negative nosocomial bacteria at Al Zahra hospital, Isfahan, Iran
CONCLUSION: Carbapenemase-positive Gram-negative bacteria causing nosocomial infections are common in our hospital and have high rate of resistance to most antibiotics. Improvement in the pattern of antibiotic use and infection control measures are necessary to overcome this resistance.PMID:33889362 | PMC:PMC8043821 | DOI:10.18502/ijm.v13i1.5492 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Sima Bahrami Fatemeh Shafiee Atousa Hakamifard Hossein Fazeli Rasool Soltani Source Type: research

Insight into population structure of < em > Mycobacterium tuberculosis < /em > isolates in the multiethnic province of Alborz, Iran
CONCLUSION: The greater heterogeneity observed here by MLVA-VNTR analysis is most likely due to limited background data in the study region rather than a genuine more heterogeneous population compared to other provinces of the country.PMID:33889363 | PMC:PMC8043826 | DOI:10.18502/ijm.v13i1.5493 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Morassa Sadat Farnad Hassan Momtaz Nader Mosavari Yahya Khosravi Keyvan Tadayon Source Type: research

Bovine subclinical mastitis-associated methicillin-resistant < em > Staphylococcus aureus < /em > , selective genotyping and antimicrobial susceptibility profile of the isolates in Kurdistan province of Iran
CONCLUSION: The detrimental involvement of S. aureus in bovine subclinical mastitis is proved herein. Besides, the contribution of MRSA and potential contamination of milk and dairy products with the bacterium may impose a serious public health risk. This demands serious and long-lasting efforts to control the infection. The results may be effective in the implementation of accurate controlling strategies.PMID:33889364 | PMC:PMC8043834 | DOI:10.18502/ijm.v13i1.5494 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Fereshteh Khazaie Elham Ahmadi Source Type: research

Antimicrobial and antibiofilm effects of < em > Satureja hortensis < /em > essential oil against < em > Escherichia coli < /em > and < em > Salmonella < /em > isolated from poultry
CONCLUSION: Based on our results, S. hortensis essential oil showed the growth inhibition and bactericidal activity against E. coli and Salmonella. Moreover, this study demonstrated anti-biofilm activity of S. hortensis essential against both tested bacteria.PMID:33889365 | PMC:PMC8043823 | DOI:10.18502/ijm.v13i1.5495 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Mohammad Haji Seyedtaghiya Bahar Nayeri Fasaei Seyed Mostafa Peighambari Source Type: research

The effects of heat-killed < em > Tsukamurella inchonensis < /em > on intestinal morphology and humoral immune responses of broiler chickens
In this study, the efficacy of heat-killed Tsukamurella inchonensis on growth performance, intestinal morphology, and humoral immune responses of broiler chicken was evaluated.MATERIALS AND METHODS: Ross broiler chicks in the cage were randomly allocated to five groups. Trail diets were prepared by adding 106 cells per bird of heat-killed T. inchonensis into the basal trading diet for group 1 continuously dosed for 24 h from day 1 to day 13, and for group 2, 24 h on days 1 to 5; 8; 9, 12 and 13. Group 3 was received 106 bacteria as a subcutaneous injection on days 1, 6, and 12. Groups 4 and 5 were not received T. inchonens...
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Katayoon Nofouzi Sorayya Mirzazadeh Monireh Khordadmehr Mohammad Sadegh Madadi Soheila Amininia Masoumeh Firouzamandi Vahid Reza Ranjbar Source Type: research

Interaction between the probiotic < em > Bacillus subtilis < /em > and < em > Salmonella < /em > Typhimurium in Caco-2 cell culture
Iran J Microbiol. 2021 Feb;13(1):91-97. doi: 10.18502/ijm.v13i1.5497.ABSTRACTBACKGROUND AND OBJECTIVES: Bacillus probiotics have been recently considered in biotechnological researches, and food additives. The present study was aimed to investigate the effects of Bacillus subtilis probiotics (PY79 and ATCC 6633) and their metabolites on Salmonella Typhimurium in Caco-2 cells.MATERIALS AND METHODS: Cytotoxicity of B. subtilis ATCC 6633 crude supernatant (CS) was evaluated by 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. S. Typhimurium invasion assay was performed in the presence of the probiotic...
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Raheleh Ostovan Maryam Pourmontaseri Saeid Hosseinzadeh Seyed Shahram Shekarforoush Source Type: research

Antimicrobial activity of bioactive compounds of < em > Haplopappus multifolius < /em > and < em > Haplopappus taeda < /em > against human pathogenic microorganisms
CONCLUSION: H. multifolius and H. taeda have antibacterial capacity on different species of Gram-positive bacteria pathogenic for humans.PMID:33889368 | PMC:PMC8043824 | DOI:10.18502/ijm.v13i1.5498 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Carlos Padilla Olga Lobos Patricia Poblete-Tapia Ver ónica Carrasco-Sánchez Source Type: research

The antibacterial effect of < em > Magnolia < /em > mouthwash on the levels of salivary < em > Streptococcus mutans < /em > in dental plaque: a randomized, single-blind, placebo-controlled trial
Iran J Microbiol. 2021 Feb;13(1):104-111. doi: 10.18502/ijm.v13i1.5499.ABSTRACTBACKGROUND AND OBJECTIVES: Dental caries is one of the most common chronic diseases around the world. Inhibitory effects of Magnolia Grandiflora bark extract has been proved on tooth decay both in vitro and by using free sugar chewing gum. This research aimed to examine the effect of Magnolia Grandiflora bark mouth-wash on the prevalence of Streptococcus mutans in dental plaque.MATERIALS AND METHODS: This crossover, placebo-controlled, clinical trial study, was performed on a total of twenty participants (aged 18 to 35 years) in both control and...
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Fateme Ghorbani Roza Haghgoo Hamed Aramjoo Hassan Rakhshandeh Saeid Amel Jamehdar Majid Zare-Bidaki Source Type: research

Antibacterial effect of carbon nanotube containing chemical compounds on drug-resistant isolates of < em > Acinetobacter baumannii < /em >
CONCLUSION: The engineered carbon nanotube compound can potentially be used for treatment of burn related infections. This can potentially give clinicians a new tool for treating A. baumannii infections.PMID:33889370 | PMC:PMC8043832 | DOI:10.18502/ijm.v13i1.5501 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Kamelia Banihashemi Nour Amirmozafari Iraj Mehregan Ronak Bakhtiari Behnam Sobouti Source Type: research

Selective screening and characterization of plant growth promoting bacteria for growth enhancement of tomato, < em > Lycopersicon esculentum < /em >
CONCLUSION: Isolation of specific PGPBS is recommended for sustainable plant production. Our results showed that NBF12 improves tomato plant growth and its effect on tomato plant growth is does dependent. Maximum growth rate of tomato was observed with 107 CFUs/g soil inoculation of NFB12 while higher inoculation showed negative effect.PMID:33889371 | PMC:PMC8043818 | DOI:10.18502/ijm.v13i1.5502 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Shiva Yavarian Parvaneh Jafari Neda Akbari Mohammad Mehdi Feizabadi Source Type: research

Diversity, activity, and effectiveness of < em > Rhizobium < /em > bacteria as plant growth promoting rhizobacteria (PGPR) isolated from Dieng, central Java
CONCLUSION: These isolates can be developed as biological fertilizer agents for the peanut plant.PMID:33889372 | PMC:PMC8043825 | DOI:10.18502/ijm.v13i1.5504 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Sri Purwaningsih Dwi Agustiyani Satjiya Antonius Source Type: research

High resolution melting assay in discrimination of the main etiologic agents of leishmaniasis in Iran
CONCLUSION: PCR-HRM analysis of kDNA proved to be fast and accurate for discrimination of L. major, L. tropica, and L. infantum.PMID:33889373 | PMC:PMC8043822 | DOI:10.18502/ijm.v13i1.5505 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Shima Fayaz Pezhman Fard-Esfahani Fariborz Bahrami Parviz Parvizi Soheila Ajdary Source Type: research

Laboratory diagnosis of COVID-19: current status and challenges
Iran J Microbiol. 2021 Feb;13(1):1-7. doi: 10.18502/ijm.v13i1.5485.ABSTRACTThe magnitude and pace of global affliction caused by Coronavirus Disease-19 (COVID-19) is unprecedented in the recent past. From starting in a busy seafood market in the Chinese city of Wuhan, the virus has spread across the globe in less than a year, infecting over 76 million people and causing death of close to 1.7 million individuals worldwide. As no specific antiviral treatment is currently available, the major strategy in containing the pandemic is focused on early diagnosis and prompt isolation of the infected individuals. Several diagnostic ...
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Bijina J Mathew Ashish Kumar Vyas Prashant Khare Sudheer Gupta Ram Kumar Nema Shashwati Nema Sudipti Gupta Shivendra K Chaurasiya Debasis Biswas Anirudh K Singh Source Type: research

Differential alteration trend of white blood cells (WBCs) and monocytes count in severe and non-severe COVID-19 patients within a 7-day follow-up
CONCLUSION: Alteration of monocytes either on admission or within hospitalization would not provide valuable data about the prediction of COVID-19 prognosis. Although the rapidly evolving nature of COVID-19 is the major limitation of the present study, further investigations in the field of laboratory biomarkers will pave the way to manage patients with severe disease better.PMID:33889357 | PMC:PMC8043835 | DOI:10.18502/ijm.v13i1.5486 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Ali Pirsalehi Sina Salari Ahmadreza Baghestani Ghazal Sanadgol Dorsa Shirini Maryam Moghbel Baerz Saeed Abdi Mohammad Esmaeil Akbari Davood Bashash Source Type: research

Transcriptome based analysis of apoptosis genes in chickens co-infected with avian infectious bronchitis virus and pathogenic < em > Escherichia coli < /em >
CONCLUSION: All of the apoptotic genes in our study were up-regulated. Among these genes, the more fold change value was for TRADD and BCL2A1 genes, and the less fold change value was for MAP3K14, NFKB1, PIK3CB, and ITPR2 genes.PMID:33889358 | PMC:PMC8043830 | DOI:10.18502/ijm.v13i1.5487 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Shabnam Hashemi Seyed Masoud Hosseini Arash Ghalyanchilangeroudi Nariman Sheikhi Source Type: research

Clinical characteristics and molecular detection of < em > Bordetella pertussis < /em > in hospitalized children with a clinical diagnosis of whooping cough in Peru
CONCLUSION: In conclusion, in the studied population there is a high rate of PCR+ cases for B. pertussis. Laboratory values may show leukopenia or lymphopenia in patients with pertussis. It is necessary to use appropriate laboratory diagnostic tests in all infants with respiratory symptoms for B. pertussis. Since, the clinical diagnosis overestimates the diagnosis of pertussis.PMID:33889359 | PMC:PMC8043828 | DOI:10.18502/ijm.v13i1.5488 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Juana Del Valle-Mendoza Cristina Del Valle-Vargas Ronald Aquino-Ortega Luis J Del Valle Erico Cieza-Mora Wilmer Silva-Caso Jorge Baz án-Mayra Victor Zavaleta-Gavidia Miguel Angel Aguilar-Luis Hern án Cornejo-Pacherres Johanna Martins-Luna Angela Cornejo Source Type: research

Comparison of PCR and phenotypic methods for the detection of methicillin resistant < em > Staphylococcus aureus < /em >
CONCLUSION: Based on the findings in our study, isolates which show cefoxitin zone diameter
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Anitha Madhavan Arun Sachu Anukumar Balakrishnan Anu Vasudevan Sobha Balakrishnan Jayalakshmi Vasudevapanicker Source Type: research

Effect of azithromycin and phenylalanine-arginine beta-naphthylamide on quorum sensing and virulence factors in clinical isolates of < em > Pseudomonas aeruginosa < /em >
CONCLUSION: Anti-QS agents like AZM and EPI (PAβN) are useful therapeutic options for P. aeruginosa due to its inhibitory effect on QS-dependent virulence factors production without selective pressure on bacteria growth, so resistance to these agents is less likely to develop.PMID:33889361 | PMC:PMC8043820 | DOI:10.18502/ijm.v13i1.5491 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Amel Elsheredy Ingy El-Soudany Eglal Elsherbini Dalia Metwally Abeer Ghazal Source Type: research

Antimicrobial susceptibility pattern of carbapenemase-producing Gram-negative nosocomial bacteria at Al Zahra hospital, Isfahan, Iran
CONCLUSION: Carbapenemase-positive Gram-negative bacteria causing nosocomial infections are common in our hospital and have high rate of resistance to most antibiotics. Improvement in the pattern of antibiotic use and infection control measures are necessary to overcome this resistance.PMID:33889362 | PMC:PMC8043821 | DOI:10.18502/ijm.v13i1.5492 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Sima Bahrami Fatemeh Shafiee Atousa Hakamifard Hossein Fazeli Rasool Soltani Source Type: research

Insight into population structure of < em > Mycobacterium tuberculosis < /em > isolates in the multiethnic province of Alborz, Iran
CONCLUSION: The greater heterogeneity observed here by MLVA-VNTR analysis is most likely due to limited background data in the study region rather than a genuine more heterogeneous population compared to other provinces of the country.PMID:33889363 | PMC:PMC8043826 | DOI:10.18502/ijm.v13i1.5493 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Morassa Sadat Farnad Hassan Momtaz Nader Mosavari Yahya Khosravi Keyvan Tadayon Source Type: research

Bovine subclinical mastitis-associated methicillin-resistant < em > Staphylococcus aureus < /em > , selective genotyping and antimicrobial susceptibility profile of the isolates in Kurdistan province of Iran
CONCLUSION: The detrimental involvement of S. aureus in bovine subclinical mastitis is proved herein. Besides, the contribution of MRSA and potential contamination of milk and dairy products with the bacterium may impose a serious public health risk. This demands serious and long-lasting efforts to control the infection. The results may be effective in the implementation of accurate controlling strategies.PMID:33889364 | PMC:PMC8043834 | DOI:10.18502/ijm.v13i1.5494 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Fereshteh Khazaie Elham Ahmadi Source Type: research

Antimicrobial and antibiofilm effects of < em > Satureja hortensis < /em > essential oil against < em > Escherichia coli < /em > and < em > Salmonella < /em > isolated from poultry
CONCLUSION: Based on our results, S. hortensis essential oil showed the growth inhibition and bactericidal activity against E. coli and Salmonella. Moreover, this study demonstrated anti-biofilm activity of S. hortensis essential against both tested bacteria.PMID:33889365 | PMC:PMC8043823 | DOI:10.18502/ijm.v13i1.5495 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Mohammad Haji Seyedtaghiya Bahar Nayeri Fasaei Seyed Mostafa Peighambari Source Type: research

The effects of heat-killed < em > Tsukamurella inchonensis < /em > on intestinal morphology and humoral immune responses of broiler chickens
In this study, the efficacy of heat-killed Tsukamurella inchonensis on growth performance, intestinal morphology, and humoral immune responses of broiler chicken was evaluated.MATERIALS AND METHODS: Ross broiler chicks in the cage were randomly allocated to five groups. Trail diets were prepared by adding 106 cells per bird of heat-killed T. inchonensis into the basal trading diet for group 1 continuously dosed for 24 h from day 1 to day 13, and for group 2, 24 h on days 1 to 5; 8; 9, 12 and 13. Group 3 was received 106 bacteria as a subcutaneous injection on days 1, 6, and 12. Groups 4 and 5 were not received T. inchonens...
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Katayoon Nofouzi Sorayya Mirzazadeh Monireh Khordadmehr Mohammad Sadegh Madadi Soheila Amininia Masoumeh Firouzamandi Vahid Reza Ranjbar Source Type: research

Interaction between the probiotic < em > Bacillus subtilis < /em > and < em > Salmonella < /em > Typhimurium in Caco-2 cell culture
Iran J Microbiol. 2021 Feb;13(1):91-97. doi: 10.18502/ijm.v13i1.5497.ABSTRACTBACKGROUND AND OBJECTIVES: Bacillus probiotics have been recently considered in biotechnological researches, and food additives. The present study was aimed to investigate the effects of Bacillus subtilis probiotics (PY79 and ATCC 6633) and their metabolites on Salmonella Typhimurium in Caco-2 cells.MATERIALS AND METHODS: Cytotoxicity of B. subtilis ATCC 6633 crude supernatant (CS) was evaluated by 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. S. Typhimurium invasion assay was performed in the presence of the probiotic...
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Raheleh Ostovan Maryam Pourmontaseri Saeid Hosseinzadeh Seyed Shahram Shekarforoush Source Type: research

Antimicrobial activity of bioactive compounds of < em > Haplopappus multifolius < /em > and < em > Haplopappus taeda < /em > against human pathogenic microorganisms
CONCLUSION: H. multifolius and H. taeda have antibacterial capacity on different species of Gram-positive bacteria pathogenic for humans.PMID:33889368 | PMC:PMC8043824 | DOI:10.18502/ijm.v13i1.5498 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Carlos Padilla Olga Lobos Patricia Poblete-Tapia Ver ónica Carrasco-Sánchez Source Type: research

The antibacterial effect of < em > Magnolia < /em > mouthwash on the levels of salivary < em > Streptococcus mutans < /em > in dental plaque: a randomized, single-blind, placebo-controlled trial
Iran J Microbiol. 2021 Feb;13(1):104-111. doi: 10.18502/ijm.v13i1.5499.ABSTRACTBACKGROUND AND OBJECTIVES: Dental caries is one of the most common chronic diseases around the world. Inhibitory effects of Magnolia Grandiflora bark extract has been proved on tooth decay both in vitro and by using free sugar chewing gum. This research aimed to examine the effect of Magnolia Grandiflora bark mouth-wash on the prevalence of Streptococcus mutans in dental plaque.MATERIALS AND METHODS: This crossover, placebo-controlled, clinical trial study, was performed on a total of twenty participants (aged 18 to 35 years) in both control and...
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Fateme Ghorbani Roza Haghgoo Hamed Aramjoo Hassan Rakhshandeh Saeid Amel Jamehdar Majid Zare-Bidaki Source Type: research

Antibacterial effect of carbon nanotube containing chemical compounds on drug-resistant isolates of < em > Acinetobacter baumannii < /em >
CONCLUSION: The engineered carbon nanotube compound can potentially be used for treatment of burn related infections. This can potentially give clinicians a new tool for treating A. baumannii infections.PMID:33889370 | PMC:PMC8043832 | DOI:10.18502/ijm.v13i1.5501 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Kamelia Banihashemi Nour Amirmozafari Iraj Mehregan Ronak Bakhtiari Behnam Sobouti Source Type: research

Selective screening and characterization of plant growth promoting bacteria for growth enhancement of tomato, < em > Lycopersicon esculentum < /em >
CONCLUSION: Isolation of specific PGPBS is recommended for sustainable plant production. Our results showed that NBF12 improves tomato plant growth and its effect on tomato plant growth is does dependent. Maximum growth rate of tomato was observed with 107 CFUs/g soil inoculation of NFB12 while higher inoculation showed negative effect.PMID:33889371 | PMC:PMC8043818 | DOI:10.18502/ijm.v13i1.5502 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Shiva Yavarian Parvaneh Jafari Neda Akbari Mohammad Mehdi Feizabadi Source Type: research

Diversity, activity, and effectiveness of < em > Rhizobium < /em > bacteria as plant growth promoting rhizobacteria (PGPR) isolated from Dieng, central Java
CONCLUSION: These isolates can be developed as biological fertilizer agents for the peanut plant.PMID:33889372 | PMC:PMC8043825 | DOI:10.18502/ijm.v13i1.5504 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Sri Purwaningsih Dwi Agustiyani Satjiya Antonius Source Type: research

High resolution melting assay in discrimination of the main etiologic agents of leishmaniasis in Iran
CONCLUSION: PCR-HRM analysis of kDNA proved to be fast and accurate for discrimination of L. major, L. tropica, and L. infantum.PMID:33889373 | PMC:PMC8043822 | DOI:10.18502/ijm.v13i1.5505 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Shima Fayaz Pezhman Fard-Esfahani Fariborz Bahrami Parviz Parvizi Soheila Ajdary Source Type: research

Laboratory diagnosis of COVID-19: current status and challenges
Iran J Microbiol. 2021 Feb;13(1):1-7. doi: 10.18502/ijm.v13i1.5485.ABSTRACTThe magnitude and pace of global affliction caused by Coronavirus Disease-19 (COVID-19) is unprecedented in the recent past. From starting in a busy seafood market in the Chinese city of Wuhan, the virus has spread across the globe in less than a year, infecting over 76 million people and causing death of close to 1.7 million individuals worldwide. As no specific antiviral treatment is currently available, the major strategy in containing the pandemic is focused on early diagnosis and prompt isolation of the infected individuals. Several diagnostic ...
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Bijina J Mathew Ashish Kumar Vyas Prashant Khare Sudheer Gupta Ram Kumar Nema Shashwati Nema Sudipti Gupta Shivendra K Chaurasiya Debasis Biswas Anirudh K Singh Source Type: research

Differential alteration trend of white blood cells (WBCs) and monocytes count in severe and non-severe COVID-19 patients within a 7-day follow-up
CONCLUSION: Alteration of monocytes either on admission or within hospitalization would not provide valuable data about the prediction of COVID-19 prognosis. Although the rapidly evolving nature of COVID-19 is the major limitation of the present study, further investigations in the field of laboratory biomarkers will pave the way to manage patients with severe disease better.PMID:33889357 | PMC:PMC8043835 | DOI:10.18502/ijm.v13i1.5486 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Ali Pirsalehi Sina Salari Ahmadreza Baghestani Ghazal Sanadgol Dorsa Shirini Maryam Moghbel Baerz Saeed Abdi Mohammad Esmaeil Akbari Davood Bashash Source Type: research

Transcriptome based analysis of apoptosis genes in chickens co-infected with avian infectious bronchitis virus and pathogenic < em > Escherichia coli < /em >
CONCLUSION: All of the apoptotic genes in our study were up-regulated. Among these genes, the more fold change value was for TRADD and BCL2A1 genes, and the less fold change value was for MAP3K14, NFKB1, PIK3CB, and ITPR2 genes.PMID:33889358 | PMC:PMC8043830 | DOI:10.18502/ijm.v13i1.5487 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Shabnam Hashemi Seyed Masoud Hosseini Arash Ghalyanchilangeroudi Nariman Sheikhi Source Type: research

Clinical characteristics and molecular detection of < em > Bordetella pertussis < /em > in hospitalized children with a clinical diagnosis of whooping cough in Peru
CONCLUSION: In conclusion, in the studied population there is a high rate of PCR+ cases for B. pertussis. Laboratory values may show leukopenia or lymphopenia in patients with pertussis. It is necessary to use appropriate laboratory diagnostic tests in all infants with respiratory symptoms for B. pertussis. Since, the clinical diagnosis overestimates the diagnosis of pertussis.PMID:33889359 | PMC:PMC8043828 | DOI:10.18502/ijm.v13i1.5488 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Juana Del Valle-Mendoza Cristina Del Valle-Vargas Ronald Aquino-Ortega Luis J Del Valle Erico Cieza-Mora Wilmer Silva-Caso Jorge Baz án-Mayra Victor Zavaleta-Gavidia Miguel Angel Aguilar-Luis Hern án Cornejo-Pacherres Johanna Martins-Luna Angela Cornejo Source Type: research

Comparison of PCR and phenotypic methods for the detection of methicillin resistant < em > Staphylococcus aureus < /em >
CONCLUSION: Based on the findings in our study, isolates which show cefoxitin zone diameter
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Anitha Madhavan Arun Sachu Anukumar Balakrishnan Anu Vasudevan Sobha Balakrishnan Jayalakshmi Vasudevapanicker Source Type: research

Effect of azithromycin and phenylalanine-arginine beta-naphthylamide on quorum sensing and virulence factors in clinical isolates of < em > Pseudomonas aeruginosa < /em >
CONCLUSION: Anti-QS agents like AZM and EPI (PAβN) are useful therapeutic options for P. aeruginosa due to its inhibitory effect on QS-dependent virulence factors production without selective pressure on bacteria growth, so resistance to these agents is less likely to develop.PMID:33889361 | PMC:PMC8043820 | DOI:10.18502/ijm.v13i1.5491 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Amel Elsheredy Ingy El-Soudany Eglal Elsherbini Dalia Metwally Abeer Ghazal Source Type: research

Antimicrobial susceptibility pattern of carbapenemase-producing Gram-negative nosocomial bacteria at Al Zahra hospital, Isfahan, Iran
CONCLUSION: Carbapenemase-positive Gram-negative bacteria causing nosocomial infections are common in our hospital and have high rate of resistance to most antibiotics. Improvement in the pattern of antibiotic use and infection control measures are necessary to overcome this resistance.PMID:33889362 | PMC:PMC8043821 | DOI:10.18502/ijm.v13i1.5492 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Sima Bahrami Fatemeh Shafiee Atousa Hakamifard Hossein Fazeli Rasool Soltani Source Type: research

Insight into population structure of < em > Mycobacterium tuberculosis < /em > isolates in the multiethnic province of Alborz, Iran
CONCLUSION: The greater heterogeneity observed here by MLVA-VNTR analysis is most likely due to limited background data in the study region rather than a genuine more heterogeneous population compared to other provinces of the country.PMID:33889363 | PMC:PMC8043826 | DOI:10.18502/ijm.v13i1.5493 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Morassa Sadat Farnad Hassan Momtaz Nader Mosavari Yahya Khosravi Keyvan Tadayon Source Type: research

Bovine subclinical mastitis-associated methicillin-resistant < em > Staphylococcus aureus < /em > , selective genotyping and antimicrobial susceptibility profile of the isolates in Kurdistan province of Iran
CONCLUSION: The detrimental involvement of S. aureus in bovine subclinical mastitis is proved herein. Besides, the contribution of MRSA and potential contamination of milk and dairy products with the bacterium may impose a serious public health risk. This demands serious and long-lasting efforts to control the infection. The results may be effective in the implementation of accurate controlling strategies.PMID:33889364 | PMC:PMC8043834 | DOI:10.18502/ijm.v13i1.5494 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Fereshteh Khazaie Elham Ahmadi Source Type: research

Antimicrobial and antibiofilm effects of < em > Satureja hortensis < /em > essential oil against < em > Escherichia coli < /em > and < em > Salmonella < /em > isolated from poultry
CONCLUSION: Based on our results, S. hortensis essential oil showed the growth inhibition and bactericidal activity against E. coli and Salmonella. Moreover, this study demonstrated anti-biofilm activity of S. hortensis essential against both tested bacteria.PMID:33889365 | PMC:PMC8043823 | DOI:10.18502/ijm.v13i1.5495 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Mohammad Haji Seyedtaghiya Bahar Nayeri Fasaei Seyed Mostafa Peighambari Source Type: research

The effects of heat-killed < em > Tsukamurella inchonensis < /em > on intestinal morphology and humoral immune responses of broiler chickens
In this study, the efficacy of heat-killed Tsukamurella inchonensis on growth performance, intestinal morphology, and humoral immune responses of broiler chicken was evaluated.MATERIALS AND METHODS: Ross broiler chicks in the cage were randomly allocated to five groups. Trail diets were prepared by adding 106 cells per bird of heat-killed T. inchonensis into the basal trading diet for group 1 continuously dosed for 24 h from day 1 to day 13, and for group 2, 24 h on days 1 to 5; 8; 9, 12 and 13. Group 3 was received 106 bacteria as a subcutaneous injection on days 1, 6, and 12. Groups 4 and 5 were not received T. inchonens...
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Katayoon Nofouzi Sorayya Mirzazadeh Monireh Khordadmehr Mohammad Sadegh Madadi Soheila Amininia Masoumeh Firouzamandi Vahid Reza Ranjbar Source Type: research

Interaction between the probiotic < em > Bacillus subtilis < /em > and < em > Salmonella < /em > Typhimurium in Caco-2 cell culture
Iran J Microbiol. 2021 Feb;13(1):91-97. doi: 10.18502/ijm.v13i1.5497.ABSTRACTBACKGROUND AND OBJECTIVES: Bacillus probiotics have been recently considered in biotechnological researches, and food additives. The present study was aimed to investigate the effects of Bacillus subtilis probiotics (PY79 and ATCC 6633) and their metabolites on Salmonella Typhimurium in Caco-2 cells.MATERIALS AND METHODS: Cytotoxicity of B. subtilis ATCC 6633 crude supernatant (CS) was evaluated by 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. S. Typhimurium invasion assay was performed in the presence of the probiotic...
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Raheleh Ostovan Maryam Pourmontaseri Saeid Hosseinzadeh Seyed Shahram Shekarforoush Source Type: research

Antimicrobial activity of bioactive compounds of < em > Haplopappus multifolius < /em > and < em > Haplopappus taeda < /em > against human pathogenic microorganisms
CONCLUSION: H. multifolius and H. taeda have antibacterial capacity on different species of Gram-positive bacteria pathogenic for humans.PMID:33889368 | PMC:PMC8043824 | DOI:10.18502/ijm.v13i1.5498 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Carlos Padilla Olga Lobos Patricia Poblete-Tapia Ver ónica Carrasco-Sánchez Source Type: research

The antibacterial effect of < em > Magnolia < /em > mouthwash on the levels of salivary < em > Streptococcus mutans < /em > in dental plaque: a randomized, single-blind, placebo-controlled trial
Iran J Microbiol. 2021 Feb;13(1):104-111. doi: 10.18502/ijm.v13i1.5499.ABSTRACTBACKGROUND AND OBJECTIVES: Dental caries is one of the most common chronic diseases around the world. Inhibitory effects of Magnolia Grandiflora bark extract has been proved on tooth decay both in vitro and by using free sugar chewing gum. This research aimed to examine the effect of Magnolia Grandiflora bark mouth-wash on the prevalence of Streptococcus mutans in dental plaque.MATERIALS AND METHODS: This crossover, placebo-controlled, clinical trial study, was performed on a total of twenty participants (aged 18 to 35 years) in both control and...
Source: Iranian Journal of Microbiology - April 23, 2021 Category: Microbiology Authors: Fateme Ghorbani Roza Haghgoo Hamed Aramjoo Hassan Rakhshandeh Saeid Amel Jamehdar Majid Zare-Bidaki Source Type: research