Significance of conformation changes during the binding and release of chromium(III) from human serum transferrin.
Abstract Trivalent chromium has been proposed to be transported in vivo from the bloodstream to the tissues via endocytosis by transferrin (Tf), the major iron transport protein in the blood. While both Cr(III) binding and release from Tf have been proposed to be too slow to be physiologically relevant, recent kinetic studies under physiological conditions demonstrate that Cr(III) binding and release are sufficiently fast to occur during the time of the endocytosis cycle (circa 15 min). Consequently, the release of Cr(III) from human and bovine serum Tf has been examined under conditions mimicking an endosome...
Source: Journal of Inorganic Biochemistry - February 15, 2020 Category: Biochemistry Authors: Edwards KC, Kim H, Ferguson R, Lockart MM, Vincent JB Tags: J Inorg Biochem Source Type: research

Anionic versus neutral Pt(II) complexes: The relevance of the charge for human serum albumin binding.
Abstract The focus of this work is pointing out the different behavior of two structurally related Pt(II) complexes, the anionic cyclometalated NBu4[(Bzq)Pt(Thio)], 1 and the neutral [(Phen)Pt(Thio)], 2, (Bzq = benzo[h]quinoline, Phen = 1,10-phenantroline, Thio = 1,2-benzenedithiolate), on the interaction with human serum albumin (HSA), a key drug-delivery protein in the bloodstream. Being very limited the number of anionic Pt(II) complexes reported to date, this is a pioneering example of report on a protein-ligand interaction involving a negatively charged platinum compound. The stu...
Source: Journal of Inorganic Biochemistry - February 11, 2020 Category: Biochemistry Authors: Ricciardi L, Guzzi R, Rizzuti B, Ionescu A, Aiello I, Ghedini M, La Deda M Tags: J Inorg Biochem Source Type: research

Novel triorganotin complexes based on phosphonic acid ligands: Syntheses, structures and in vitro cytostatic activity.
Abstract Six novel organotin phosphonate complexes, [(Me3Sn)4(HL1)4]n1, [(Me3Sn)2(HL2)2]n2, [(Me3Sn)2L3(H2O)]n3, [(Ph3Sn)(HL1)]64, [(Ph3Sn)2L2]n5 and [(Ph3Sn)2L3]66, derived from phosphonic acid ligands [NaHL1 = 1-C10H7OPO2(OH)Na, H2L2 = 1-C10H7PO(OH)2, H2L3 = 2-C10H7PO(OH)2], have been synthesized and characterized by elemental analysis, FT-IR, NMR (1H, 13C, 31P and 119Sn) spectroscopy and X-ray crystallography. The structural analysis reveals that complexes 1 and 5 display 1D infinite zig-zag chain structures, and complex 2 shows 1D right-handed helical chain structure, while comple...
Source: Journal of Inorganic Biochemistry - February 8, 2020 Category: Biochemistry Authors: An BH, Zhang RF, Du XM, Li QL, Cheng S, Huang HL, Ma CL Tags: J Inorg Biochem Source Type: research

The structure of the oxidized state of cytochrome c oxidase - experiments and theory compared.
Abstract Cytochrome c oxidase (CcO), the terminal enzyme in the respiratory chain, reduces molecular oxygen to water. Experimental data on the midpoint potentials of the heme iron/copper active site cofactors do not match the overall reaction energetics, and are also in conflict with the observed efficiency of energy conservation in CcO. Therefore it has been postulated that the ferric/cupric intermediate (the oxidized state) exists in two forms. One form, labelled OH, is presumably involved during catalytic turnover, and should have a high CuB midpoint potential due to a metastable high energy structure. When no ...
Source: Journal of Inorganic Biochemistry - February 8, 2020 Category: Biochemistry Authors: Blomberg MRA Tags: J Inorg Biochem Source Type: research

The FeII(citrate) Fenton reaction under physiological conditions.
ein D Abstract The Fenton reaction of FeII(citrate) in the presence and absence of bicarbonate (HCO3-) is studied. It is found that the rate constant of the Fenton reaction (kobs) increases with increasing [citrate]. kobs also increase with increasing [HCO3-]; this effect is most significant at biological citrate concentrations. Methane and ethane gases are formed from (CH3)2SO when the Fenton reaction is carried out in the presence of large [citrate] due to the reaction of the citrate radical, (-2OC)CH2C(OH)(CO2-)CH(CO2-)/(-2OC)CH2C(O)(CO2-)CH2(CO2-) with (CH3)2SO. In the absence of citrate (CH3)2SO2 is the main ...
Source: Journal of Inorganic Biochemistry - February 4, 2020 Category: Biochemistry Authors: Illés E, Patra SG, Marks V, Mizrahi A, Meyerstein D Tags: J Inorg Biochem Source Type: research

Liposomes encapsulated iridium(III) polypyridyl complexes enhance anticancer activity in vitro and in vivo.
Abstract Three iridium(III) complexes [Ir(ppy)2(CPIP)](PF6) (Ir-1, ppy = 2-phenylpyridine, CPIP = 2-(4-chlorophenyl)-1H-imidazo[4,5-f][1,10]phenanthroline), [Ir(ppy)2(DCPIP)](PF6) (Ir-2, DCPIP = 2-(3,4-dichlorophenyl)-1H-imidazo[4,5-f][1,10]phenanthroline) and [Ir(ppy)2(TCPIP)](PF6) (Ir-3, TCPIP = 2,3,5-trichlorophenyl)-1H-imidazo[4,5-f][1,10]phenanthroline) were synthesized and characterized. The complexes Ir-1, Ir-2 and Ir-3 were encapsulated in liposomes to form Ir-1-Lipo, Ir-2-Lipo and Ir-3-Lipo. Morphology, size distribution, and zeta potential of liposomes were examine...
Source: Journal of Inorganic Biochemistry - January 31, 2020 Category: Biochemistry Authors: Bai L, Fei WD, Gu YY, He M, Du F, Zhang WY, Yang LL, Liu YJ Tags: J Inorg Biochem Source Type: research

Novel nickel(II), palladium(II), and platinum(II) complexes having a pyrrolyl-iminophosphine (PNN) pincer: Synthesis, crystal structures, and cytotoxic activity.
Abstract A pyrrolyl-iminophosphine (PNNH) which would act as a potential terdentate ligand has been prepared by Schiff base reaction. Complexes [M(PNN)X] (M = Ni; X = Cl (1), Pd; X = Cl (2), Br (3), I (4), M = Pt; X = Cl (5)) were prepared. The title complexes were characterized by various spectroscopic (IR, 1H, 13C, and 31P NMR) and elemental analyses. The molecular structures of 1, 2, and 5 have been established by single-crystal X-ray crystallography, demonstrating a distorted square planar geometry comprising two 5-membered metallacyclic rings. Complexes 1 and ...
Source: Journal of Inorganic Biochemistry - January 30, 2020 Category: Biochemistry Authors: Kim Y, Lee J, Son YH, Choi SU, Alam M, Park S Tags: J Inorg Biochem Source Type: research

The assembly and antitumor activity of lycium barbarum polysaccharide-platinum-based conjugates.
Abstract In this work, the new polysaccharide-platinum conjugates of 5-aminosalicylic acid modified lycium barbarum polysaccharide linking platinum compounds were designed in order to construct an anticancer metal drug delivery system. The multiple analysis methods were used to describe the chemical structure and physical properties of the polysaccharide-metal conjugates. The results showed that 5-aminosalicylic acid successfully acted as linker which was covalently bound between polysaccharide and platinum compound. The morphology and rheological properties of polysaccharide have been changed by the formation of ...
Source: Journal of Inorganic Biochemistry - January 24, 2020 Category: Biochemistry Authors: Wang Y, Han Q, Bai F, Luo Q, Wu M, Song G, Zhang H, Wang Y Tags: J Inorg Biochem Source Type: research

Synthesis, crystal structure and leishmanicidal activity of new trimethoprim Ru(III), Cu(II) and Pt(II) metal complexes.
riguetto AC Abstract Leishmaniasis is a parasitic disease caused by protozoa of the genus Leishmania, which has very limited treatment options and affects poor and underdeveloped populations. The current treatment is plagued by many complications, such as high toxicity, high cost and resistance to parasites; therefore, novel therapeutic agents are urgently needed. Herein, the synthesis, characterization and in vitro leishmanicidal potential of new complexes with the general formula [RuCl3(TMP)(dppb)] (1), [PtCl(TMP)(PPh3)2]PF6 (2) and [Cu(CH3COO)2(TMP)2]·DMF (3) (dppb = 1,4-bis(diphenylphosphino)b...
Source: Journal of Inorganic Biochemistry - January 23, 2020 Category: Biochemistry Authors: Silva GL, Dias JSM, Silva HVR, Teixeira JDS, De Souza IRB, Guimarães ET, de Magalhães Moreira DR, Soares MBP, Barbosa MIF, Doriguetto AC Tags: J Inorg Biochem Source Type: research

Iridium(III) coordination of N(6) modified adenine derivatives with aminoacid chains.
cute;zquez-López EM, Fiol JJ Abstract In this manuscript we report the preparation of three N6-aminoacid-adenine-derivatives: N-(7H-purin-6-yl)glycine·0.5H2O (N6-GlyAde), N-(7H-purin-6-yl)-β-alanine·1.5H2O (N6-β-AlaAde) and N-(7H-purin-6-yl)-γ-aminobutyric·2H2O (N6-GabaAde) and the synthesis and X-ray characterization of three Ir(III) NAMI-A derivatives (NAMI-A is [imidazoleH][trans-RuIIICl4(DMSO-κS)(imidazole)]) [trans-IrIIICl4(DMSO-κS)(N3-H)-(7H-purin-6-yl)glycine-κN9] (1), [trans-IrIIICl4(DMSO-κS)(N3-H)-(7H-purin-6-yl)-β-alanine-κN9]...
Source: Journal of Inorganic Biochemistry - January 18, 2020 Category: Biochemistry Authors: García-Raso A, Terrón A, Ortega-Castro J, Barceló-Oliver M, Lorenzo J, Rodríguez-Calado S, Franconetti A, Frontera A, Vázquez-López EM, Fiol JJ Tags: J Inorg Biochem Source Type: research

Cytotoxic Ag(I) and Au(I) NHC-carbenes bind DNA and show TrxR inhibition.
ani C Abstract A silver(I) and a gold(I) complex of the fluorescent N-heterocyclic carbenic (NHC) ligand 1-(9-anthracenylmethyl)-3-(3-trimethylsilyl-2-propynil)-benzimidazol-2-ylidene have been synthesized and characterized. These compounds show cytotoxicity in the micromolar range and higher antiproliferative properties than cisplatin (CDDP) against several tumour cell lines such as SW480 (colon), A549 (lung) and HepG2 (liver). Both metal complexes are successfully internalized by SW480 cells being the silver compound the most accumulated. Subsequently, they were evaluated as inhibitors of the selenoenzyme Thiore...
Source: Journal of Inorganic Biochemistry - January 15, 2020 Category: Biochemistry Authors: Guarra F, Busto N, Guerri A, Marchetti L, Marzo T, García B, Biver T, Gabbiani C Tags: J Inorg Biochem Source Type: research

Multiple drug binding modes in Mycobacterium tuberculosis CYP51B1.
Abstract The Mycobacterium tuberculosis (Mtb) genome encodes 20 different cytochrome P450 enzymes (CYPs), many of which serve essential biosynthetic roles. CYP51B1, the Mtb version of eukaryotic sterol demethylase, remains a potential therapeutic target. The binding of three drug fragments containing nitrogen heterocycles to CYP51B1 is studied here by continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) techniques to determine how each drug fragment binds to the heme active-site. All three drug fragments form a mixture of complexes, some of which retain the axial water ligand from the resting stat...
Source: Journal of Inorganic Biochemistry - January 13, 2020 Category: Biochemistry Authors: Lockart MM, Butler JT, Mize CJ, Fair MN, Cruce AA, Conner KP, Atkins WM, Bowman MK Tags: J Inorg Biochem Source Type: research

Tau/A β chimera peptides: Evaluating the dual function of metal coordination and membrane interaction in one sequence.
In this study, we assess the amyloidogenic propensity and membrane-damage effects, either in the presence or in absence of metal ions, of two newly synthesized bifunctional peptides. These were designed to comprise a metal chelating N-terminus region derived from Tau protein namely the Tau9-16 (EVMEDHAG) or Tau26-33 (QGGYTMHQ) sequences, merged with the C-terminal hydrophobic region analogous to the Amyloid beta (Aβ) 16-20 aminoacid sequence KLVFF (KL). Comparative circular dichroism or fluorescence experiments were carried out to look at the peptide conformation, fibril formation and membrane affinity of Tau9-16KL an...
Source: Journal of Inorganic Biochemistry - January 11, 2020 Category: Biochemistry Authors: Sciacca MFM, Di Natale G, Tosto R, Milardi D, Pappalardo G Tags: J Inorg Biochem Source Type: research

Ditopic binuclear copper(II) complexes for DNA cleavage.
an-Gómez D Abstract Herein we present the synthesis of two ligands containing two di(2-picolyl)amine (DPA) units linked by either a 1,1'-(pyridine-2,6-diyl)bis(3-ethylurea) (L1) or a 1,1'-(1,3-phenylene)bis(3-ethylurea) (L2) spacer. The corresponding binuclear CuII and ZnII complexes were prepared and isolated. The X-ray structures of the L1 ligand and the [Cu2L1Cl2]2+ complex evidence an unusual cis/trans conformation of one of the urea groups stabilized by an intramolecular hydrogen bond with the nitrogen atom of the pyridyl spacer. The CuII complexes form rather strong ternary complexes with phosphorylat...
Source: Journal of Inorganic Biochemistry - January 11, 2020 Category: Biochemistry Authors: Carreira-Barral I, Riopedre-Fernández M, de Blas A, Mosquera J, Vázquez ME, Platas-Iglesias C, Esteban-Gómez D Tags: J Inorg Biochem Source Type: research

Dual functions of iridium(III) 2-phenylpyridine complexes: Metastasis inhibition and lysosomal damage.
Abstract Six N-phenylcarbazole/triphenylamine-appended half-sandwich iridium(III) 2-phenylpyridine complexes ([(η5-Cp*)Ir(C^N)Cl]) were prepared and characterized. Compared with cisplatin, these complexes exhibited potential antitumor activity against A549 and HeLa tumor cells, with IC50 values (half-maximum inhibitory concentration) that changed from 2.8 ± 0.8 μM to 39.5 ± 2.7 μM, and could block the migration of tumor cells. These complexes also effectively bound to protein (binding constant: ~104 M-1) and were transported through serum proteins, catalyz...
Source: Journal of Inorganic Biochemistry - January 8, 2020 Category: Biochemistry Authors: Liu X, Chen S, Ge X, Zhang Y, Xie Y, Hao Y, Wu D, Zhao J, Yuan XA, Tian L, Liu Z Tags: J Inorg Biochem Source Type: research

Biological sulfur in the blood cells of Ascidia ceratodes: XAS spectroscopy and a cellular-enzymatic hypothesis for vanadium reduction in the ascidians.
Abstract Two samples of living blood cells and of cleared blood plasma from the Phlebobranch tunicate Ascidia ceratodes from Bodega Bay, California, and one of fresh Henze solution from A. ceratodes of Monterey Bay, California, have been examined using sulfur K-edge x-ray absorption spectroscopy (XAS). Biological sulfur included sulfate esters, sulfate and bisulfate ions, benzothiazole, thianthrene, epi-sulfide, thiol and disulfide. Glutathione dominated reduced sulfur, from which an average intracellular Voltage of -0.21 V was calculated. Sulfate-bisulfate ratios yielded blood cell pH values of 2.0 and 2.8. Total...
Source: Journal of Inorganic Biochemistry - January 7, 2020 Category: Biochemistry Authors: Frank P, Carlson RMK, Carlson EJ, Hedman B, Hodgson KO Tags: J Inorg Biochem Source Type: research

Oxidative damage under As3+ and/or Cu2+ stress leads to apoptosis and autophagy and may be cross-talking with mitochondrial disorders in bursa of Fabricius.
In conclusion, apoptosis and autophagy of BF induced by As3+ and Cu2+ and mitochondrial disorder are closely related to the collapse of antioxidant system, and their connections are inseparable. Our results provide a reference for environmental risk prevention and selection of poultry feed additives and pesticides to avoid the health risks caused by As3+ and Cu2+ exposure. PMID: 31945648 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - January 7, 2020 Category: Biochemistry Authors: Guo M, Wang Y, Zhao H, Mu M, Yang X, Fei D, Liu Y, Zong H, Xing M Tags: J Inorg Biochem Source Type: research

Cu(II) coordination to His-containing linear peptides and related branched ones: Equalities and diversities.
Abstract The two branched peptides (AAHAWG)4-PWT2 and (HAWG)4-PWT2 where synthesized by mounting linear peptides on a cyclam-based scaffold (PWT2), provided with four maleimide chains, through a thio-Michael reaction. The purpose of this study was primarily to verify if the two branched ligands had a Cu(II) coordination behavior reproducing that of the single-chain peptides, namely AAHAWG-NH2, which bears an Amino Terminal Cu(II)- and Ni(II)-Binding (ATCUN) Motif, and HAWG-NH2, which presents a His residue as the N-terminal amino acid, in a wide pH range. The study of Cu(II) binding was performed by potentiometric...
Source: Journal of Inorganic Biochemistry - January 3, 2020 Category: Biochemistry Authors: Perinelli M, Guerrini R, Albanese V, Marchetti N, Bellotti D, Gentili S, Tegoni M, Remelli M Tags: J Inorg Biochem Source Type: research

Exploring the interactions of iron and zinc with the microtubule binding repeats R1 and R4.
Abstract The dyshomeostasis of copper, iron and zinc ions in pathological conditions, which are critically involved in many brain activities, may result in an accumulation of them in the brain that has been reported for the patients with Alzheimer's disease. Conformational change is one of the consequences of metal-peptide interaction as we observed for the interaction of the Cu2+ with microtubule binding repeats of tau protein, which ultimately cause peptide aggregation. Herein, we show that interaction of Zn2+, Fe2+, and Fe3+ with full-length tau peptide R1 (tau244-274) and R4 (tau337-368), the first and fourth ...
Source: Journal of Inorganic Biochemistry - December 31, 2019 Category: Biochemistry Authors: Ahmadi S, Wu B, Song R, Zhu S, Simpson A, Wilson DJ, Kraatz HB Tags: J Inorg Biochem Source Type: research

Formation and kinetic studies of manganese(IV)-oxo porphyrins: Oxygen atom transfer mechanism of sulfide oxidations.
Abstract Visible light irradiation of photo-labile porphyrin-manganese(III) chlorates or bromates (2) produced manganese(IV)-oxo porphyrins [MnIV(Por)(O)] (Por = porphyrin) (3) in three porphyrin ligands. The same oxo species 3 were also formed by chemical oxidation of the corresponding manganese(III) precursors (1) with iodobenzene diacetate, i.e. PhI(OAc)2. The systems under study include 5,10,15,20-tetra(pentafluorophenyl)porphyrin‑manganese(IV)-oxo (3a), 5,10,15,20-tetra(2,6-difluorophenyl)porphyrin‑manganese(IV)-oxo (3b), and 5,10,15,20-tetramesitylporphyrin‑manganese(IV)-oxo (3c). As expected...
Source: Journal of Inorganic Biochemistry - December 31, 2019 Category: Biochemistry Authors: Klaine S, Bratcher F, Winchester CM, Zhang R Tags: J Inorg Biochem Source Type: research

Metal complexes for mitochondrial bioimaging.
Abstract Mitochondria are essential organelles in eukaryotic cells, containing various signaling molecules and important enzymes associated with cell growth, death, and proliferation. The visualization of mitochondria and their biochemistry with confocal microscopy, fluorescence (phosphorescence) lifetime microscopy (FLIM, PLIM), and super-resolution microscopy has therefore been of great interest in recent years. In particular, transition metal complexes have emerged as excellent mitochondria-targeting probes, due to their high photostabilities, large Stokes shifts, tunable chemical structures and long luminescen...
Source: Journal of Inorganic Biochemistry - December 30, 2019 Category: Biochemistry Authors: Guan R, Xie L, Rees TW, Ji L, Chao H Tags: J Inorg Biochem Source Type: research

A trans-dichloridoplatinum(II) complex of a monodentate nitrogen mustard: Synthesis, stability and cytotoxicity studies.
Abstract A trans-dichloridoplatinum(II) complex, trans-[PtIICl2(L)(DMSO)] (1) of a monodentate nitrogen mustard, bis(2-chloroethyl)amine (L), was synthesized by the reaction of cis-[PtIICl2(DMSO)2] &L.HCl in presence of Et3N. 1 was characterised by NMR, FT-IR and elemental analysis. L is unstable in aqueous solution while 1 displayed moderate stability. In aqueous buffer solution of pD 7.4, 1 starts to loose L slowly upon dissolution and even after 48 h there is still intact/aquated complex present in solution. 1 interacts with the model nucleobase 9-ethyl guanine. The ligand L was non-toxic against MCF-7...
Source: Journal of Inorganic Biochemistry - December 28, 2019 Category: Biochemistry Authors: Karmakar S, Chatterjee S, Purkait K, Mukherjee A Tags: J Inorg Biochem Source Type: research

Anticancer and antimetastatic activity of copper(II)-tropolone complex against human breast cancer cells, breast multicellular spheroids and mammospheres.
;n IE Abstract The goal of this work was to display the anticancer and antimetastatic activity of a copper(II) with tropolone (trp), complex [Cu(trp)2] toward human breast cancer cells in monolayer (2D) and spheroids (3D). Cytotoxicity assays against MCF7 (IC50(complex) = 5.2 ± 1.8 μM, IC50(CDDP) = 19.3 ± 2.1 μM) and MDA-MB-231 (IC50(complex) = 4.0 ± 0.2 μM, IC50(CDDP) = 27.0 ± 1.9 μM) demonstrate that [Cu(trp)2] exert greater antitumor potency than cisplatin (CDDP) on 2D and ...
Source: Journal of Inorganic Biochemistry - December 26, 2019 Category: Biochemistry Authors: Balsa LM, Ruiz MC, Santa Maria de la Parra L, Baran EJ, León IE Tags: J Inorg Biochem Source Type: research

The influence of land-use on tropical soil chemical characteristics with emphasis on aluminium.
PD, Lojka B, Němeček K, Borůvka L, Drábek O Abstract Composition of soil vegetation cover and land management directly influences the cycling of chemical elements and is a key factor for soil biogeochemistry and also Al behaviour. Moreover, Al is an important factor limiting the growth of cultural plants. Our results are based on long-term observations of soils translocated from selected small areas of eight 1 ha plots of different land-use gradient, with identical geological, climatic and geographical conditions, located in the North of Congo Basin (near Mbalmayo, Cameroon). The plots are established ...
Source: Journal of Inorganic Biochemistry - December 23, 2019 Category: Biochemistry Authors: Tejnecký V, Křížová P, Penížek V, Maňourová A, Sillam-Dussès D, Šobotník J, Akama PD, Lojka B, Němeček K, Borůvka L, Drábek O Tags: J Inorg Biochem Source Type: research

Synthesis, characterization and albumin binding capabilities of quinizarin containing ternary cobalt(III) complexes.
cute;A, Buglyó P Abstract Four Co(III) ternary complexes with the composition of [(Co(4 N))2(quin)](ClO4)4 or [(Co(4 N))2(quinS)](ClO4)3, where 4 N = tris(2-aminoethyl)amine (tren) or tris(2-pyridylmethyl)amine (tpa), quinH2 = quinizarin (1,4-dihydroxy-9,10-anthraquinone), quinSH3 = quinizarin-2-sulfonic acid (1,4-dihydroxy-9,10-anthraquinone-2-sulfonic acid), were synthesized, characterized and their human serum albumin (HSA) binding capabilities were also tested. The complexes can be considered as likely chaperons of quinizarins which are structural models for ...
Source: Journal of Inorganic Biochemistry - December 17, 2019 Category: Biochemistry Authors: Kozsup M, Dömötör O, Nagy S, Farkas E, Enyedy ÉA, Buglyó P Tags: J Inorg Biochem Source Type: research

Urease inhibition studies of six Ni(II), Co(II) and Cu(II) complexes with two sexidentate N2O4-donor bis-Schiff base ligands: An experimental and DFT computational study.
Abstract Six novel complexes, [Ni(C36H34N2O10)]·2.25CH3OH·0.5C4H10O (1), [Co(C36H34N2O10)] (2), [Cu(C36H34N2O10)]·2CH3OH (3), [Ni(C36H32N2O8Cl2)]·2CH3OH (4), [Co(C36H32N2O8Cl2)]·4CH3OH (5) and [Cu(C36H32N2O8Cl2)]·2CH3OH (6) with two sexidentate N2O4-donor bis-Schiff base ligands (C36H34N2O10 = 1,2-bis(2-methoxy-6-formylphenoxy)ethane-l-tyrosine; C36H32N2O8Cl2 = 1,2-bis(2-methoxy-6-formylphenoxy)ethane-l-4-chlorophenylalanine) have been synthesized and structurally characterized. Theoretical calculation of the six complexes was carried out by density fun...
Source: Journal of Inorganic Biochemistry - December 16, 2019 Category: Biochemistry Authors: Wang H, Xu C, Zhang X, Zhang D, Jin F, Fan Y Tags: J Inorg Biochem Source Type: research

Aluminium alters mineral composition and polyphenol metabolism in leaves of tea plants (Camellia sinensis).
In conclusion, low pH and enhanced Al availability to tea plants have a strong influence on the polyphenolic pattern of tealeaves and therefore may alter both the leaves' antioxidant properties and their ability to bind Al and Fe in non-toxic form. PMID: 31862583 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - December 12, 2019 Category: Biochemistry Authors: Tolrà R, Martos S, Hajiboland R, Poschenrieder C Tags: J Inorg Biochem Source Type: research

Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles.
Abstract The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV-Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorptio...
Source: Journal of Inorganic Biochemistry - December 11, 2019 Category: Biochemistry Authors: Laban B, Ralević U, Petrović S, Leskovac A, Vasić-Anićijević D, Marković M, Vasić V Tags: J Inorg Biochem Source Type: research

Gallium-68 and scandium-44 labelled radiotracers based on curcumin structure linked to bifunctional chelators: Synthesis and characterization of potential PET radiotracers.
i M Abstract Curcumin metal complexes showed widespread applications in medicine and can be exploited as a lead structure for developing new tracers for nuclear medicine application. Herein, the synthesis, chemical characterization and radiolabelling with gallium-68 and scandium-44 of two new targeting vectors based on curcumin scaffolds and linked to the chelators 1,4,7-triazacyclononane,1-glutaric acid-4,7-acetic acid (NODAGA) and 1,4-bis(carboxymethyl)-6-[bis(carboxymethyl)]amino-6-methylperhydro-1,4-diazepine (AAZTA) are reported. Synthesis of the precursors could be achieved with a 13% and 11% yield and radio...
Source: Journal of Inorganic Biochemistry - December 5, 2019 Category: Biochemistry Authors: Orteca G, Sinnes JP, Rubagotti S, Iori M, Capponi PC, Piel M, Rösch F, Ferrari E, Asti M Tags: J Inorg Biochem Source Type: research

Structural zinc binding sites shaped for greater works: Structure-function relations in classical zinc finger, hook and clasp domains.
Abstract Metal ions are essential elements present in biological systems able to facilitate many cellular processes including proliferation, signaling, DNA synthesis and repair. Zinc ion (Zn(II)) is an important cofactor for numerous biochemical reactions. Commonly, structural zinc sites demonstrate high Zn(II) affinity and compact architecture required for sequence-specific macromolecule binding. However, how Zn(II)-dependent proteins fold, how their dissociation occurs, and which factors modulate zinc protein affinity as well as stability remains not fully understood. The molecular rules governing precise regula...
Source: Journal of Inorganic Biochemistry - December 5, 2019 Category: Biochemistry Authors: Padjasek M, Kocyła A, Kluska K, Kerber O, Tran JB, Krężel A Tags: J Inorg Biochem Source Type: research

Triplet of cysteines - Coordinational riddle?
In this study, the interaction of Zn2+, Cd2+ and Ni2+ ions with four peptides containing cysteine triplet motif were studied by potentiometric and spectroscopic methods. The main goal of this research was to answer the question how effectively three thiols, each being next to other, are able to bind single metal ion. Two of peptides contain additional, fourth cysteine residue, separated from triplet by two and three other amino acid residues. As results show, all three cysteine residues in the CCC motif are able to participate in the coordination of the metal ion (Cd2+, Zn2+). Except cysteine thiol groups, amide nitrogen a...
Source: Journal of Inorganic Biochemistry - December 5, 2019 Category: Biochemistry Authors: Krzywoszyńska K, Swiątek-Kozłowska J, Potocki S, Ostrowska M, Kozłowski H Tags: J Inorg Biochem Source Type: research

Ternary copper(II) complex of 5-hydroxytryptophan and 1,10-phenanthroline with several pharmacological properties and an adequate safety profile.
We report the synthesis and biological evaluation of a ternary copper complex, [Cu(5HTP)(phen)(H2O)](NO3).2H2O, with the antioxidant agent 5-hydroxytryptophan (5-HTP) and phenanthroline (phen, added to improve its lipophilicity and membrane transport). The crystal structure of the complex was determined by X-ray diffraction methods. The complex showed antioxidant, antimicrobial, antitumor and antimetastatic properties with an adequate safety profile. The interaction of the metal with phen promotes cellular copper accumulation and cytotoxicity on human lung A549 cell line (IC50 = 3.6 μM). Furthermore, the ...
Source: Journal of Inorganic Biochemistry - December 5, 2019 Category: Biochemistry Authors: Naso LG, Martínez Medina JJ, D'Alessandro F, Rey M, Rizzi A, Piro OE, Echeverría GA, Ferrer EG, Williams PAM Tags: J Inorg Biochem Source Type: research

DFT analysis, spectroscopic study and biological activity of a newly synthesized benzoylhydrazone binuclear Cu(II) complex.
Abstract The current work reports a joint experimental and theoretical study of a novel Cu(II) complex [CuL(μ-CH3COO)]2, based on 2-acetylpyridine-benzoylhydrazone ligand (HL). The two Cu(II) atoms are five-coordinated, consisting of three NNO-donor atoms from the hidrazone ligand connected by acetate bridges. In addition to the structural analysis, the complete characterization includes magnetic susceptibility, elemental analysis, FT-IR and UV-Vis. Two different approaches of Density Functional Theory (DFT) with localized basis set and plane waves were performed. The theoretical calculations were used to optim...
Source: Journal of Inorganic Biochemistry - November 29, 2019 Category: Biochemistry Authors: Santiago PHO, Tiago FS, Castro MS, Souza PEN, Martins JBL, Gatto CC Tags: J Inorg Biochem Source Type: research

Anti-leishmanial activity and cytotoxicity of a series of tris-aryl Sb(V) mandelate cyclometallate complexes.
Abstract A series of ten cyclometallates and two μ2-peroxo bridged tris-aryl Sb(V) complexes derived from R/S-mandelic acid (= R/S-ManH2) were synthesised and characterised. As confirmed by X-ray crystallography the complexes 1Sr/s, [Sb(o-tol)3(man)], 2Sr/s, [Sb(m-tol)3(man)], 4Sr/s, [Sb(o-PhOMe)3(man)], 5Sr/s, [Sb(Mes)3(man)] and 6Sr/s, [Sb(p-tert-BuPh)3(man)] are all cyclometallates. Complexes 3Sr/s, [(Sb(p-tol)3(manH)2O2], contain a bridging O22- anion in the solid-state but convert to the cyclometallates in DMSO solution with concomitant release of H2O2 and formation of complexes [Sb(p-tol)3(man)], 3Sr'/s'....
Source: Journal of Inorganic Biochemistry - November 23, 2019 Category: Biochemistry Authors: Duffin RN, Blair VL, Kedzierski L, Andrews PC Tags: J Inorg Biochem Source Type: research

Influence of synthesis methods on the internalization of fluorescent gold nanoparticles into glioblastoma stem-like cells.
D, Janiak C Abstract Glioblastoma (GBM) is an aggressive disease with currently no satisfying treatment option available. GBM cells with stem cell properties are thought to be responsible for the initiation and propagation of the disease, as well as main contributors to the emergence of therapy resistance. In this work, we developed a novel method to synthesize fluorescent gold nanoparticles as potential drug and gene delivery systems for GBM therapy, able to penetrate three-dimensional stem cell selected patient-derived GBM neurosphere systems in vitro. By using polyethylene imine (PEI) as a stabilizer and reduci...
Source: Journal of Inorganic Biochemistry - November 23, 2019 Category: Biochemistry Authors: Giesen B, Nickel AC, Garzón Manjón A, Vargas Toscano A, Scheu C, Kahlert UD, Janiak C Tags: J Inorg Biochem Source Type: research

Modified EDTA selectively recognized Cu2+ and its application in the disaggregation of β-amyloid-Cu (II)/Zn (II) aggregates.
Modified EDTA selectively recognized Cu2+ and its application in the disaggregation of β-amyloid-Cu (II)/Zn (II) aggregates. J Inorg Biochem. 2019 Nov 23;203:110929 Authors: Chang R, Chen X, Yu H, Tan G, Wen H, Huang J, Hao Z Abstract The accumulation of the β-amyloid (Aβ) aggregates induced by Cu2+/Zn2+ in conjunction with toxicity is closely related to Alzheimer's disease (AD). Herein, we intended to improve the efficiency and selectivity of traditional chelator ethylenediaminetetraacetic acid (EDTA) combined with a fluorescent group 4-aminosalicylic acid (4-ASA)to acquire a novel pot...
Source: Journal of Inorganic Biochemistry - November 23, 2019 Category: Biochemistry Authors: Chang R, Chen X, Yu H, Tan G, Wen H, Huang J, Hao Z Tags: J Inorg Biochem Source Type: research

Comparative study of the antitumoral activity of phosphine-thiosemicarbazone gold(I) complexes obtained by different methodologies.
aacute;lez-Noya AM, Pedrido R Abstract A series of phosphino-thiosemicarbazone gold(I) dinuclear complexes obtained by two different synthetic procedures have been prepared. All the compounds have been spectroscopically characterized including single crystal X ray diffraction analysis in some of cases. [Au2(HL1)Cl2] (1), [Au2(HL2)2]Cl2 (2) and [Au2(HL3)2]Cl2 (3) have been prepared by chemical synthesis using a gold(III) salt as precursor; while [Au2(L1)2] (4), [Au2(L2)2]∙2CH3CN (5) and [Au2(L3)2] (6) have been isolated from an electrochemical synthesis (HLn = 2-[2-(diphenylphosphanyl)-benzylidene]-N-R-thiose...
Source: Journal of Inorganic Biochemistry - November 22, 2019 Category: Biochemistry Authors: González-Barcia LM, Fernández-Fariña S, Rodríguez-Silva L, Bermejo MR, González-Noya AM, Pedrido R Tags: J Inorg Biochem Source Type: research

Coordination chemistry and catalytic applications of Pd(II)-, and Ni(II)-sulfosalan complexes in aqueous media.
te; F Abstract With the aim of identifying new types of water-soluble catalyst precursors for modification of biological membranes by homogeneous hydrogenation in aqueous solution and under mild conditions, we have performed detailed equilibrium and spectroscopic characterization of complex formation between nickel(II) or palladium(II) and salan-type ligands sulfonated in their aromatic rings (N,N'-bis(2-hydroxy-5-sulfonatobenzyl)-1,4-diaminoethane (HSS), N,N'-bis(2-hydroxy-5-sulfonatobenzyl)-1,4-diaminopropane (PrHSS) and N,N'-bis(2-hydroxy-5-sulfonatobenzyl)-1,4-diaminobutane (BuHSS)) in the slightly acidic-alka...
Source: Journal of Inorganic Biochemistry - November 22, 2019 Category: Biochemistry Authors: Lihi N, Bunda S, Udvardy A, Joó F Tags: J Inorg Biochem Source Type: research

Cognition and behavior in sheep repetitively inoculated with aluminum adjuvant-containing vaccines or aluminum adjuvant only.
This study is the first to describe behavioral changes in sheep after having received repetitive injections of Al-containing products, and may explain some of the clinical signs observed in ovine ASIA syndrome. PMID: 31783216 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - November 20, 2019 Category: Biochemistry Authors: Asín J, Pascual-Alonso M, Pinczowski P, Gimeno M, Pérez M, Muniesa A, de Pablo-Maiso L, de Blas I, Lacasta D, Fernández A, de Andrés D, Reina R, Luján L Tags: J Inorg Biochem Source Type: research

Importance of the iron-sulfur component and of the siroheme modification in the resting state of sulfite reductase.
cu R Abstract The active site of sulfite reductase (SiR) consists of an unusual siroheme-Fe4S4 assembly coupled via a cysteinate sulfur, and serves for multi-electron reduction reactions. Clear explanations have not been demonstrated for the reasons behind the choice of siroheme (vs. other types of heme) or for the single-atom coupling to an Fe4S4 center (as opposed to simple adjacency or to coupling via chains consisting of more than one atom). Possible explanations for these choices have previously been invoked, relating to the control of the spin state of the substrate-binding (siro)heme iron, modulation of the...
Source: Journal of Inorganic Biochemistry - November 18, 2019 Category: Biochemistry Authors: Brânzanic AMV, Ryde U, Silaghi-Dumitrescu R Tags: J Inorg Biochem Source Type: research

Copper(I) complexes with phosphines P(p-OCH3-Ph)2CH2OH and P(p-OCH3-Ph)2CH2SarGly. Synthesis, multimodal DNA interactions, and prooxidative and in vitro antiproliferative activity.
oł A Abstract Phosphonium salt (p-OCH3-Ph)2P(CH2OH)2Cl (MPOHC), derived phosphine ligands without and with SarGly (Sarcosine-Glycine) peptide carrier P(p-OCH3-Ph)2CH2OH (MPOH) and P(p-OCH3-Ph)2CH2SarGly (MPSG), respectively, and two copper(I) complexes [Cu(I)(dmp)(MPOH)] (1-MPOH; dmp = (2,9-dimethyl-1,10-phenanthroline)) and [Cu(I)(dmp)(MPSG)] (1-MPSG) were synthesized. The resulting compounds were characterized by elemental analysis, 1D and 2D NMR and UV-Vis absorption spectroscopies, mass spectrometry, cyclic voltammetry and by X-ray diffraction analysis. Cytotoxicity of all compounds was evaluated in...
Source: Journal of Inorganic Biochemistry - November 15, 2019 Category: Biochemistry Authors: Komarnicka UK, Kozieł S, Zabierowski P, Kruszyński R, Lesiów MK, Tisato F, Porchia M, Kyzioł A Tags: J Inorg Biochem Source Type: research

Salen ‑manganese complexes for controlling ROS damage: Neuroprotective effects, antioxidant activity and kinetic studies.
Salen‑manganese complexes for controlling ROS damage: Neuroprotective effects, antioxidant activity and kinetic studies. J Inorg Biochem. 2019 Nov 14;203:110918 Authors: Rouco L, Liberato A, Fernández-Trujillo MJ, Máñez A, Basallote MG, Alvariño R, Alfonso A, Botana LM, Maneiro M Abstract A new manganese(III) complex [MnL1(DCA)(H2O)](H2O),1 [H2L1 is the chelating ligand N,N'-bis(2-hydroxy-3-methoxybenzylidene)-1,2-diaminopropane, and DCA is dicyanamide], has been prepared and characterized by different analytical and spectroscopic techniques. The tetragonally elongated oc...
Source: Journal of Inorganic Biochemistry - November 14, 2019 Category: Biochemistry Authors: Rouco L, Liberato A, Fernández-Trujillo MJ, Máñez A, Basallote MG, Alvariño R, Alfonso A, Botana LM, Maneiro M Tags: J Inorg Biochem Source Type: research

Investigation of the requirements for efficient and selective cytochrome P450 monooxygenase catalysis across different reactions.
Abstract The cytochrome P450 metalloenzyme (CYP) CYP199A4 from Rhodopseudomonas palustris HaA2 catalyzes the highly efficient oxidation of para-substituted benzoic acids. Here we determined crystal structures of CYP199A4, and the binding and turnover parameters, with different meta-substituted benzoic acids in order to establish which criteria are important for efficient catalysis. When compared to the para isomers, the meta-substituted benzoic acids were less efficiently oxidized. For example, 3-formylbenzoic acid was oxidized with lower activity than the equivalent para isomer and 3-methoxybenzoic acid did not u...
Source: Journal of Inorganic Biochemistry - November 13, 2019 Category: Biochemistry Authors: Podgorski MN, Coleman T, Chao RR, De Voss JJ, Bruning JB, Bell SG Tags: J Inorg Biochem Source Type: research

The interaction of aluminium-based adjuvants with THP-1 macrophages in vitro: Implications for cellular survival and systemic translocation.
Abstract Within clinical vaccinations, recombinant antigens are routinely entrapped inside or adsorbed onto the surface of aluminium salts in order to increase their immunological potency in vivo. The efficacy of these immunisations is highly dependent upon the recognition and uptake of these complexes by professional phagocytes and their subsequent delivery to the draining lymph nodes for further immunological processing. While monocytes have been shown to internalise aluminium adjuvants and their adsorbates, the role of macrophages in this respect has not been fully established. Furthermore, this study explored ...
Source: Journal of Inorganic Biochemistry - November 12, 2019 Category: Biochemistry Authors: Shardlow E, Mold M, Exley C Tags: J Inorg Biochem Source Type: research

Molecular docking of polyoxometalates as potential α-glucosidase inhibitors.
Molecular docking of polyoxometalates as potential α-glucosidase inhibitors. J Inorg Biochem. 2019 Nov 12;203:110914 Authors: Hu J, Wang L, Wang F, Chi G, Liu G, Sun L Abstract α-Glucosidase is an important target enzyme for the treatment of type 2 diabetes in humans. In our previous studies, it was found that polyoxometalates exhibited an effective inhibitory effect on the activity of α-glucosidase, while polyoxometalates have the characteristics of structural diversity and unique properties. Herein, we investigated the inhibition of two different series of polyoxometalates on &alph...
Source: Journal of Inorganic Biochemistry - November 12, 2019 Category: Biochemistry Authors: Hu J, Wang L, Wang F, Chi G, Liu G, Sun L Tags: J Inorg Biochem Source Type: research

The inhibition of lactoperoxidase catalytic activity through mesna (2-mercaptoethane sodium sulfonate).
Abstract Here, we show that mesna (sodium-2-mercaptoethane sulfonate), primarily used to prevent nephrotoxicity and urinary tract toxicity caused by chemotherapeutic agents such as cyclophosphamide and ifosfamide, modulates the catalytic activity of lactoperoxidase (LPO) by binding tightly to the enzyme, functioning either as a one electron substrate for LPO Compounds I and II, destabilizing Compound III. Lactoperoxidase is a hemoprotein that utilizes hydrogen peroxide (H2O2) and thiocyanate (SCN-) to produce hypothiocyanous acid (HOSCN), an antimicrobial agent also thought to be associated with carcinogenesis. Ou...
Source: Journal of Inorganic Biochemistry - November 12, 2019 Category: Biochemistry Authors: Jahanbakhsh S, Dekhne MS, Kohan-Ghadr HR, Bai D, Awonuga A, Morris RT, Yang Z, Abu-Soud HM Tags: J Inorg Biochem Source Type: research

Exploring DNA binding ability of two novel α-N-heterocyclic thiosemicarbazone palladium(II) complexes.
Exploring DNA binding ability of two novel α-N-heterocyclic thiosemicarbazone palladium(II) complexes. J Inorg Biochem. 2019 Nov 06;203:110875 Authors: Fuentes L, Quiroga AG, Organero JA, Matesanz AI Abstract One mononuclear and another dinuclear Pd(II) complexes bearing a α-N-heterocyclic thiosemicarbazone ligand have been synthesized, fully characterized and studied as biological agents. In both complexes, the palladium center is coordinated to 3,5-diacetyl-1,2,4-triazol bis(N4,N4-dimethylthiosemicarbazone) via three sites (N, N and S). Their binding ability to DNA has been evaluated usi...
Source: Journal of Inorganic Biochemistry - November 6, 2019 Category: Biochemistry Authors: Fuentes L, Quiroga AG, Organero JA, Matesanz AI Tags: J Inorg Biochem Source Type: research

The mechanism for oxygen reduction in the C family cbb3 cytochrome c oxidases - Implications for the proton pumping stoichiometry.
Abstract Cytochrome c oxidases (CcOs) couple the exergonic reduction of molecular oxygen to proton pumping across the membrane in which they are embedded, thereby conserving a significant part of the free energy. The A family CcOs are known to pump four protons per oxygen molecule, while there is no consensus regarding the proton pumping stoichiometry for the C family cbb3 oxidases. Hybrid density functional theory is used here to investigate the catalytic mechanism for oxygen reduction in cbb3 oxidases. A surprising result is that the barrier for O O bond cleavage at the mixed valence reduction level seems to be ...
Source: Journal of Inorganic Biochemistry - October 31, 2019 Category: Biochemistry Authors: Blomberg MRA Tags: J Inorg Biochem Source Type: research

Polynuclear zinc(II) complexes of thiosemicarbazone: Synthesis, X-ray structure and biological evaluation.
Abstract Two new dimeric Zn(II) ([{ZnL1(DMSO2)}2]·DMSO (1), [{ZnL2Cl}2] (2)) and a novel tetrameric Zn(II) complex ([(Zn2L3)2(μ-OAc)2(μ3-O)2] (3)), where H2L1 = 4-(p-methoxyphenyl) thiosemicarbazone of o-hydroxynapthaldehyde, HL2 = 4-(p-methoxyphenyl)thiosemicarbazone of benzoyl pyridine and H2L3 = 4-(p-chlorophenyl)thiosemicarbazone of o-vanillin are reported. Ligands and their complexes were characterized by spectroscopic and single crystal X-ray diffraction techniques. In addition, the complexes exhibited good binding affinity towards HSA (1012 M-1), which is supported by their abilit...
Source: Journal of Inorganic Biochemistry - October 31, 2019 Category: Biochemistry Authors: Saswati, Mohanty M, Banerjee A, Biswal S, Horn A, Schenk G, Brzezinski K, Sinn E, Reuter H, Dinda R Tags: J Inorg Biochem Source Type: research

An Erlotinib gold(I) conjugate for combating triple-negative breast cancer.
uiz J Abstract An Erlotinib triphenylphosphane gold(I) conjugate has been prepared from AuCl(PPh3) and its crystal structure has been established by X-ray diffraction, showing a metallo-helicate formation. IC50 values of the new gold conjugate were calculated towards a panel of human tumor cell lines representative of breast (MCF-7, MDA-MB-231) and colon (HT-29) cancer cells. Overall, the gold conjugate exhibited higher cytotoxic activity than that of Erlotinib against the cancer cells studied. Particularly, the antiproliferative effect of the conjugate demonstrated to be 68-fold higher than Erlotinib in highly me...
Source: Journal of Inorganic Biochemistry - October 30, 2019 Category: Biochemistry Authors: Ortega E, Zamora A, Basu U, Lippmann P, Rodríguez V, Janiak C, Ott I, Ruiz J Tags: J Inorg Biochem Source Type: research