Insights on alpha lipoic and dihydrolipoic acids as promising scavengers of oxidative stress and possible chelators in mercury toxicology.
olo S Abstract Alpha lipoic acid (α-LA) and its reduced form dihydrolipoic acid (DHLA) have been historically considered as excellent anti-oxidants and oxidative stress scavengers. Upon oxidation with reactive oxygen species (ROS) and pro-oxidants, α-LA may be reconstituted from DHLA and other reduced forms. Oxidative stress is one of the fundamental causes of functional degeneration, autophagy and apoptosis leading to cytotoxicity and loss of cell survival, often due to exposure to xenobiotics, pollutants, heavy metals, and other environmental and endogenous toxicants. α-LA and DHLA can react wi...
Source: Journal of Inorganic Biochemistry - March 22, 2019 Category: Biochemistry Authors: Bjørklund G, Aaseth J, Crisponi G, Rahman MM, Chirumbolo S Tags: J Inorg Biochem Source Type: research

The essential metals for humans: a brief overview.
Abstract The human body needs about 20 essential elements in order to function properly and among them, for certain, 10 are metal elements, though for every metal we do need, there is another one in our body we could do without it. Until about 1950 poor attention was given to the so-called "inorganic elements" and while researches on "organic elements" (C, N, O and H) and organic compounds were given high priority, studies on essential inorganic elements were left aside. Base on current knowledge it is ascertained today that metals such as Na, K, Mg, Ca, Fe, Mn, Co, Cu, Zn and Mo are essential ...
Source: Journal of Inorganic Biochemistry - March 21, 2019 Category: Biochemistry Authors: Zoroddu MA, Aaseth J, Crisponi G, Medici S, Peana M, Nurchi VM Tags: J Inorg Biochem Source Type: research

Zinc-oxaprozin compounds: Synthesis, structure and biological activity.
Abstract Four novel zinc complexes, namely [Zn(oxa)2(MeOH)4] (1), [Zn(oxa)2(H2O)(bipy)]·MeOH·2.5H2O (2·MeOH·2.5H2O), [Zn(oxa)2(bipyam)]·1.25MeOH (3·1.25MeOH) and [Zn(oxa)2(phen)] (4), with the non-steroidal anti-inflammatory drug oxaprozin (Hoxa) and a N,N'-donor heterocyclic ligand, such as 2,2'‑bipyridylamine (bipyam), 1,10‑phenanthroline (phen) or 2,2'‑bipyridine (bipy), were characterized with physicochemical techniques, various spectroscopies and single-crystal X-ray crystallography. In these coordination compounds, the oxaprozin ligands are coordinated to zinc ...
Source: Journal of Inorganic Biochemistry - March 20, 2019 Category: Biochemistry Authors: Lazou M, Hatzidimitriou AG, Papadopoulos AN, Psomas G Tags: J Inorg Biochem Source Type: research

Antigiardiasic activity of Cu(II) coordination compounds: Redox imbalance and membrane damage after a short exposure time.
We present the antigiardiasic effect of eight Cu(II) coordination compounds, which belong to the family Casiopeínas. Two of them, 4,7-diphenyl-1,10-phenanthroline(acetylacetonato)copper(II) nitrate (CasIII-Ha,36 μM) and 4,7-diphenyl-1,10-phenanthroline(glycinato)copper(II) nitrate (CasI-gly,36 μM) have shown the best antiproliferative effect in Giardia intestinalis trophozoite cultures, both with the higher lipophilic character of the series. The antiproliferative effect of these coordination compounds is attributable to its capacity to interact with the cellular membrane and to increase reactive oxygen sp...
Source: Journal of Inorganic Biochemistry - March 20, 2019 Category: Biochemistry Authors: Rufino-González Y, Ponce-Macotela M, García-Ramos JC, Martínez-Gordillo MN, Galindo-Murillo R, González-Maciel A, Reynoso-Robles R, Tovar-Tovar A, Flores-Alamo M, Toledano-Magaña Y, Ruiz-Azuara L Tags: J Inorg Biochem Source Type: research

The hydrogen bonding network of coproheme in coproheme decarboxylase from Listeria monocytogenes: Effect on structure and catalysis.
h G Abstract Coproheme decarboxylase (ChdC) catalyzes the oxidative decarboxylation of coproheme to heme b, i.e. the last step in the recently described coproporphyrin-dependent pathway. Coproheme decarboxylation from Listeria monocytogenes is a robust enzymatic reaction of low catalytic efficiency. Coproheme acts as both substrate and redox cofactor activated by H2O2. It fully depends on the catalytic Y147 close to the propionyl group at position 2. In the present study we have investigated the effect of disruption of the comprehensive and conserved hydrogen bonding network between the four propionates and heme c...
Source: Journal of Inorganic Biochemistry - March 20, 2019 Category: Biochemistry Authors: Milazzo L, Gabler T, Pfanzagl V, Michlits H, Furtmüller PG, Obinger C, Hofbauer S, Smulevich G Tags: J Inorg Biochem Source Type: research

Phleomycin complex - Coordination mode and in vitro cleavage of DNA.
Abstract Phleomycin is one of the anticancer glycopeptide antibiotics which cause DNA cleavage. It is commonly used as a copper(II) complex. Therefore, it is important to study the metal ion binding process and to define the coordination mode. In this paper, we describe the acid-base properties of phleomycin and the coordination sphere of the Cu(II) cation. In the metal binding process up to five nitrogen donor atoms can be involved. Four of them in the same plane deriving from: the pyrimidine ring, secondary amine of β-aminoalanine, imidazole and amide of the nearest peptide bond (from β-hydroxyhistidin...
Source: Journal of Inorganic Biochemistry - March 20, 2019 Category: Biochemistry Authors: Stokowa-Sołtys K, Dzyhovskyi V, Wieczorek R, Jeżowska-Bojczuk M Tags: J Inorg Biochem Source Type: research

Studying the reactivity of "old" Cu(II) complexes for "novel" anticancer purposes.
Studying the reactivity of "old" Cu(II) complexes for "novel" anticancer purposes. J Inorg Biochem. 2019 Mar 18;195:51-60 Authors: Peña Q, Lorenzo J, Sciortino G, Rodríguez-Calado S, Maréchal JD, Bayón P, Simaan AJ, Iranzo O, Capdevila M, Palacios Ò Abstract Reactive oxygen species (ROS) formation appears as one of the most promising pathways to induce cell death. The interesting Cu(II)/Cu(I) redox pair has been reported to biologically generate ROS and induce cell damage. Simple metal complexes, such as cisplatin, sometimes offer even better prop...
Source: Journal of Inorganic Biochemistry - March 17, 2019 Category: Biochemistry Authors: Peña Q, Lorenzo J, Sciortino G, Rodríguez-Calado S, Maréchal JD, Bayón P, Simaan AJ, Iranzo O, Capdevila M, Palacios Ò Tags: J Inorg Biochem Source Type: research

Structures and anticancer activity of chlorido platinum(II) saccharinate complexes with mono- and dialkylphenylphosphines.
Abstract cis-[PtCl(sac)(PPh2Me)2] (1), cis-[PtCl(sac)(PPhMe2)2] (2), trans-[PtCl(sac)(PPh2Et)2] (3) and trans-[PtCl(sac)(PPhEt2)2] (4) complexes (sac = saccharinate) were synthesized and characterized by elemental analysis and spectroscopic methods. The structures of 2-4 were determined by X-ray single-crystal diffraction. The interaction of the complexes with DNA was studied various biochemical, biophysical and molecular docking methods. Only the cis-configured complexes (1 and 2) showed nuclease activity and their binding affinity towards DNA was considerably higher than those of their trans-congeners (3 and...
Source: Journal of Inorganic Biochemistry - March 13, 2019 Category: Biochemistry Authors: Icsel C, Yilmaz VT, Cevatemre B, Aygun M, Ulukaya E Tags: J Inorg Biochem Source Type: research

Structural analysis of copper(I) interaction with amyloid β peptide.
In this study we have analyzed Cu(I) interaction with the Amyloid β fragment encompassing the first 16 amino-acids. Our data were obtained by means of NMR spectroscopy which provided relevant structural details of the metal complexes. Our findings are consistent with the involvement of two or three His in the Cu(I) coordination sphere and indicate that His6 effectively participates to the metal binding. PMID: 30884319 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - March 6, 2019 Category: Biochemistry Authors: De Gregorio G, Biasotto F, Hecel A, Luczkowski M, Kozlowski H, Valensin D Tags: J Inorg Biochem Source Type: research

Increased ROS generation causes apoptosis-like death: Mechanistic insights into the anti-Leishmania activity of a potent ruthenium(II) complex.
We report, for the first time, that hmxbato and precursor promoted an increase in reactive oxygen species production, depolarization of the mitochondrial membrane, DNA fragmentation, formation of a pre-apoptotic peak, alterations in parasite morphology and formation of autophagic vacuoles. Taken together, our results suggest that these ruthenium complexes cause parasite death by apoptosis. Thus, this work provides relevant knowledge on the activity of ruthenium(II) complexes against L. (L.) amazonensis. Such information will be essential for the exploitation of these complexes as future candidates for cutaneous leishmanias...
Source: Journal of Inorganic Biochemistry - March 5, 2019 Category: Biochemistry Authors: Costa MS, Gonçalves YG, Teixeira SC, Nunes DCO, Lopes DS, da Silva CV, da Silva MS, Borges BC, Silva MJB, Rodrigues RS, Rodrigues VM, Von Poelhsitz G, Yoneyama KAG Tags: J Inorg Biochem Source Type: research

Interactions of acetamide and acrylamide with heme models: Synthesis, infrared spectra, and solid state molecular structures of five- and six-coordinate ferric porphyrin derivatives.
Abstract The amide functional group is a fundamental building block of proteins, but is also present in several industrial chemicals such as acetamide and acrylamide. Some acetamide derivatives are known to deplete cytoplasmic heme, and some acrylamide derivatives are known to cause porphyria and may activate soluble guanylyl cyclase through a heme-dependent mechanism. We have prepared a representative set of six-coordinate acetamide and acrylamide (L) complexes of iron porphyrins of the form [(por)Fe(L)2]ClO4 (por = TPP (tetraphenylporphyrinato dianion), T(p-OMe)PP (tetrakis(p-methoxyphenyl)porphyrinato diani...
Source: Journal of Inorganic Biochemistry - March 3, 2019 Category: Biochemistry Authors: Xu N, Guan Y, Nguyen N, Lingafelt C, Powell DR, Richter-Addo GB Tags: J Inorg Biochem Source Type: research

Oligomerization of cytochrome c, myoglobin, and related heme proteins by 3D domain swapping.
Abstract Oligomerization of heme proteins is useful for construction of new materials with cooperative and systematic functions; thus, diverse methods have been applied for construction of artificial heme protein oligomers. Three-dimensional (3D) domain swapping is a protein oligomerization phenomenon that exchanges the same domain or secondary structural element between molecules. 3D domain swapping was first reported in 1994; since then many proteins have been reported to domain swap. Our research group has been showing that various heme proteins domain swap. We also found that domain swapping of heme proteins o...
Source: Journal of Inorganic Biochemistry - March 2, 2019 Category: Biochemistry Authors: Hirota S Tags: J Inorg Biochem Source Type: research

Synthesis, characterization and antibacterial activity of Zn(II) coordination polymer.
In this study, the zinc(II)-based coordination polymer, [Zn(CPDA)(NO3)2)](CPDA = 1,2-cyclopentanedicarboxylic acid) (1), had been successfully synthesized according to the hydrothermal method. Afterwards, 1 had been characterized by means of single crystal and power X-ray diffraction, elemental analysis, thermogravimetric analysis and infrared spectrum techniques. In addition, the antibacterial activities in vitro had been evaluated towards Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), respectively, through the growth inhibition and inhibition zone experimental methods. Our results indicated that 1 ...
Source: Journal of Inorganic Biochemistry - February 26, 2019 Category: Biochemistry Authors: Wang D, Xing X, Ye X, Chen Z, Gou Z, Wu D Tags: J Inorg Biochem Source Type: research

Palladium(II) complexes with salicylaldehyde ligands: Synthesis, characterization, structure, in vitro and in silico study of the interaction with calf-thymus DNA and albumins.
Abstract The synthesis and characterization of four palladium(II) complexes with substituted salicylaldehydes (X-saloH) having the general formula [Pd(X-salo)2] was undertaken. The complexes are formulated as [Pd(3-OCH3-salo)2] 1, [Pd(5-NO2-salo)2] 2, [Pd(5-Cl-salo)2] 3, and [Pd(5-Br-salo)2] 4. The structure of complex 1 was verified by single-crystal X-ray crystallography. Spectroscopic (UV-vis), and physicochemical (viscosity measurements) techniques were employed in order to study the binding of the complexes with calf-thymus (CT) DNA, while ethidium bromide (EB) displacement studies, performed by fluorescence ...
Source: Journal of Inorganic Biochemistry - February 23, 2019 Category: Biochemistry Authors: Zianna A, Geromichalos GD, Hatzidimitriou AG, Coutouli-Argyropoulou E, Lalia-Kantouri M, Psomas G Tags: J Inorg Biochem Source Type: research

Anticancer activity and mechanism of bis-pyrimidine based dimetallic Ru(II)( η6-p-cymene) complex in human non-small cell lung cancer via p53-dependent pathway.
Anticancer activity and mechanism of bis-pyrimidine based dimetallic Ru(II)(η6-p-cymene) complex in human non-small cell lung cancer via p53-dependent pathway. J Inorg Biochem. 2019 Feb 23;194:52-64 Authors: Bhatti MZ, Ali A, Duong HQ, Chen J, Rahman FU Abstract Non-small cell lung cancer (NSCLC) is the most common cancer worldwide, which is related with poor prognosis and resistance to chemotherapy. Notably, ruthenium-based complexes have emerged as good alternative to the currently used platinum-based drugs for cancer therapy. In the present study, we synthesized a novel bis-pyrimidine based lig...
Source: Journal of Inorganic Biochemistry - February 22, 2019 Category: Biochemistry Authors: Bhatti MZ, Ali A, Duong HQ, Chen J, Rahman FU Tags: J Inorg Biochem Source Type: research

Half-sandwich Ru( η6-p-cymene) complexes featuring pyrazole appended ligands: Synthesis, DNA binding and in vitro cytotoxicity.
Half-sandwich Ru(η6-p-cymene) complexes featuring pyrazole appended ligands: Synthesis, DNA binding and in vitro cytotoxicity. J Inorg Biochem. 2019 Feb 23;194:74-84 Authors: Huang YC, Haribabu J, Chien CM, Sabapathi G, Chou CK, Karvembu R, Venuvanalingam P, Ching WM, Tsai ML, Hsu SCN Abstract Organometallic Ru(II)-arene complexes have emerged as potential alternatives to platinum appended agents due to their wide range of interesting features such as stability in solution and solid, significant activity, less toxicity and hydrophobic property of arene moiety, etc. Hence, a series of Ru(II)-p-cyme...
Source: Journal of Inorganic Biochemistry - February 22, 2019 Category: Biochemistry Authors: Huang YC, Haribabu J, Chien CM, Sabapathi G, Chou CK, Karvembu R, Venuvanalingam P, Ching WM, Tsai ML, Hsu SCN Tags: J Inorg Biochem Source Type: research

Development of a series of 4-hydroxycoumarin platinum(IV) hybrids as antitumor agents: Synthesis, biological evaluation and action mechanism investigation.
Abstract A series of new 4-hydroxycoumarin platinum(IV) complexes were designed, synthesized and evaluated as antitumor agents. All the title compounds display moderate to effective antitumor activities toward the tested cell lines and two prominent compounds were screened out with activities comparable to cisplatin and oxaliplatin. The mechanism investigation demonstrates that the platinum(IV) compounds could be reduced to bivalence and exert significant genotoxicity to tumor cells. Meanwhile the coumarin moiety endows the title compounds with cyclooxygenase inhibitory competence which might favour the reduction ...
Source: Journal of Inorganic Biochemistry - February 22, 2019 Category: Biochemistry Authors: Li G, Zhang J, Liu Z, Wang Q, Chen Y, Liu M, Li D, Han J, Wang B Tags: J Inorg Biochem Source Type: research

Complex formation equilibria of polyamine ligands with copper(II) and zinc(II).
z Martín A Abstract A comprehensive study of the protonation equilibria of a series of polyamine ligands along with their complex formation equilibria with Cu2+ and Zn2+ is reported in this work. The primary aim of this study has been the achievement of homogeneous thermodynamic data on these ligands, in order to evaluate their influence on the homeostatic equilibria of essential metal ions (Cu2+ and Zn2+) in biological fluids. These polyamines are largely used as linkers in the building of chelating agents for iron overload. Potentiometric and spectrophotometric techniques were used for the characterizatio...
Source: Journal of Inorganic Biochemistry - February 18, 2019 Category: Biochemistry Authors: Nurchi VM, Crisponi G, Sanna G, Pérez-Toro I, Niclos-Gutierrez J, Gonzalez-Perez MJ, Domínguez Martín A Tags: J Inorg Biochem Source Type: research

Diclofenac conjugates with biocides through silver(I) ions (CoMeD's); Development of a reliable model for the prediction of anti-proliferation of NSAID's-silver formulations.
Abstract The conjugation of diclofenac (DICLH), a Non-Steroidal Anti-inflammatory Drug (NSAID), with biocides such as dimethyl sulfoxide (DMSO) and triphenylphosphine (TPP), through silver(I) ions, results into the chemical [Agn(DICL)n(L)m]k (L = DMSO and n = 2, m = 2, k = infinite (1); L = TPP and n = 1, m = 2, k = 1 (2)). The compounds were characterized by m.p., FT-IR, UV-vis and 1H NMR spectroscopic techniques. The crystal and molecular structures of 1-2 were determined by X-ray crystallography. The in vitro cytotoxic activity of 1-2 against the human breast adenocarcinoma cance...
Source: Journal of Inorganic Biochemistry - February 17, 2019 Category: Biochemistry Authors: Banti CN, Hatzidimitriou AG, Kourkoumelis N, Hadjikakou SK Tags: J Inorg Biochem Source Type: research

Anticancer properties of a new non-oxido vanadium(IV) complex with a catechol-modified 3,3'-diindolylmethane ligand.
Abstract In order to identify new active drug candidates against cancer diseases we investigated the tumor cell growth inhibition, formation of reactive oxygen species, mitochondrial membrane damage, cell cycle arrest and DNA binding activity of a new bis(triethylammonium) tris[1,1-bis(indol-3-yl)-1-(3,4-catecholate)methane]vanadate(IV) complex. It exhibited significant antiproliferative activity against various cancer cell lines, showed a stronger DNA binding than cisplatin and led to mitochondrial damage, a formation of reactive oxygen species, and a cell cycle arrest in the G2/M phase of cancer cells. PMID...
Source: Journal of Inorganic Biochemistry - February 13, 2019 Category: Biochemistry Authors: Dankhoff K, Ahmad A, Weber B, Biersack B, Schobert R Tags: J Inorg Biochem Source Type: research

Polyoxomolybdates as α-glucosidase inhibitors: Kinetic and molecular modeling studies.
In this report, we showed that polyoxomolybdates based on Keggin-type architecture are promising candidates. Kinetic studies indicate that H3PMo12O40, Na4PMo11VO40, Na6PMo11FeO40 and Na7PMo11CoO40 strongly inhibit α-glucosidase with IC50 values of 6.14 ± 0.38 μM, 52.33 ± 1.41 μM, 161.90 ± 7.68 μM and 103.10 ± 2.88 μM, respectively. Moreover, H3PMo12O40, Na4PMo11VO40, and Na7PMo11CoO40 are reversible, competitive inhibitors with KI values of 0.018 mM, 0.146 mM and 0.121 mM, respectively. Na6PMo11FeO40 inhibited α-glucosidase in a reversible no...
Source: Journal of Inorganic Biochemistry - February 8, 2019 Category: Biochemistry Authors: Chi G, Qi Y, Li J, Wang L, Hu J Tags: J Inorg Biochem Source Type: research

A new tripodal kojic acid derivative for iron sequestration: Synthesis, protonation, complex formation studies with Fe3+, Al3+, Cu2+ and Zn2+, and in vivo bioassays.
Abstract This work presents the simple and low cost synthesis of a new tripodal ligand, in which three units of kojic acid are coupled to a tris(2-aminoethyl)amine (tren) backbone molecule. The protonation equilibria, together with the complex formation equilibria of this ligand with Fe3+, Al3+, Cu2+ and Zn2+ ions were studied. The complementary use of potentiometric, spectrophotometric and NMR techniques, and of Density Functional Theory (DFT) calculations, has allowed a thorough characterization of the different species involved in equilibrium. The stability of the formed complexes with Fe3+ and Al3+ are high en...
Source: Journal of Inorganic Biochemistry - February 6, 2019 Category: Biochemistry Authors: Nurchi VM, de Guadalupe Jaraquemada-Pelaez M, Crisponi G, Lachowicz JI, Cappai R, Gano L, Santos MA, Melchior A, Tolazzi M, Peana M, Medici S, Zoroddu MA Tags: J Inorg Biochem Source Type: research

Lycopene attenuates aluminum-induced hippocampal lesions by inhibiting oxidative stress-mediated inflammation and apoptosis in the rat.
In this study, the neuroprotective effects and mechanisms of LYC against aluminum chloride (AlCl3)-induced hippocampal lesions were explored. First, oral administration of LYC (4 mg/kg) alleviated AlCl3-induced (150 mg/kg) cognition impairment and histopathological changes of the hippocampus in rats. Then, LYC significantly attenuated AlCl3-induced oxidative stress, presenting as the reduced reactive oxygen species, malondialdehyde and 8-hydroxy-2'-deoxyguanosine levels, and increased glutathione level and superoxide dismutase activity. Moreover, LYC also protected the hippocampus from AlCl3-induced apoptosis and neuro...
Source: Journal of Inorganic Biochemistry - February 1, 2019 Category: Biochemistry Authors: Cao Z, Wang P, Gao X, Shao B, Zhao S, Li Y Tags: J Inorg Biochem Source Type: research

A cisplatin-based platinum(IV) prodrug containing a glutathione s-transferase inhibitor to reverse cisplatin-resistance in non-small cell lung cancer.
Abstract A Pt(IV) prodrug of cisplatin containing a glutathione s-transferase (GSTs) inhibitor 6-(7-nitro-2,1,3-benzoxadiazol-4-ylthio)hexanol (NBDHEX), complex 1, was designed and studied aiming to overcome cisplatin-resistance and reduce its toxicity by inhibiting GSTs overexpressed in cancer cells. The complex could be reduced to release its active Pt(II) species and axial ligand in the presence of ascorbic acid. In cytotoxicity study, complex 1 showed more potent anticancer activity than cisplatin and NBDHEX against all the tested cancer cells, especially toward cisplatin resistant A549/DDP cells with a resist...
Source: Journal of Inorganic Biochemistry - January 29, 2019 Category: Biochemistry Authors: Chen H, Wang X, Gou S Tags: J Inorg Biochem Source Type: research

Photochemical property of two Ru(II) compounds based on 5-(2-pyrazinyl)tetrazole for cancer phototherapy by changing auxiliary ligand.
Abstract Ru(II) compounds are potential candidates for photodynamic therapy (PDT) and auxiliary ligands may have an impact on the property of the resulting coordination compounds. In the present study, two Ru(II) compounds based on 5-(2-pyrazinyl)tetrazole (Hpztz) and two classic auxiliary ligands, 2,2'-bipyridine (bipy) or 1,10-phenanthroline (phen) have been prepared and characterized, namely [Ru(pztz)(bipy)2][PF6] (1) and [Ru(pztz)(phen)2][PF6] (2). The nanoparticles (NPs) of the two compounds have been prepared by self-assembly in aqueous solution. In vitro MTT assay on HeLa cells show that [Ru(pztz)(phen)2][P...
Source: Journal of Inorganic Biochemistry - January 28, 2019 Category: Biochemistry Authors: Yang J, Xu Y, Jiang M, Zou D, Yang G, Shen L, Zou J Tags: J Inorg Biochem Source Type: research

A new scheme for rational design and synthesis of polyoxovanadate hybrids with high antitumor activities.
Abstract A new strategy to construct polyoxovanadate hybrids incorporating amino acid esters in mild conditions was presented in this paper. These new hybrids were not only structurally determined by Single Crystal X-Ray diffraction, but also exhibited higher antitumor activities against laryngeal carcinoma, rhabdomyoma, and breast adenocarcinoma tumor cells compared with the traditional commercial medicine 5-fluorouracil. These results would provide a promising lead scaffold for further design and synthesis of potential anticancer agents. PMID: 30711559 [PubMed - as supplied by publisher] (Source: Journal of...
Source: Journal of Inorganic Biochemistry - January 28, 2019 Category: Biochemistry Authors: Hu X, Wang H, Huang B, Li N, Hu K, Wu B, Xiao Z, Wei Y, Wu P Tags: J Inorg Biochem Source Type: research

Ruthenium(II) salicylate complexes inducing ROS-mediated apoptosis by targeting thioredoxin reductase.
Abstract Thioredoxin reductase (TrxR), a major component of the thioredoxin system, makes a critical role in regulating cellular redox signaling and is found to be overexpressed in many human cancer cells. TrxR has become an attractive target for anticancer agents. In this work, three Ru(II) complexes with salicylate as ligand, [Ru(phen)2(SA)] (phen = 1,10-phenanthroline, SA = salicylate, 1), [Ru(dmb)2(SA)] (dmb = 4,4'-dimethyl-2,2'-bipyridine, 2) and [Ru(bpy)2(SA)] (bpy = 2,2'-bipyridine, 3), were synthesized and characterized. The anticancer effect exerted by them was evaluated. Complex 1 was fou...
Source: Journal of Inorganic Biochemistry - January 25, 2019 Category: Biochemistry Authors: Chen JC, Zhang Y, Jie XM, She J, Dongye GZ, Zhong Y, Deng YY, Wang J, Guo BY, Chen LM Tags: J Inorg Biochem Source Type: research

Synthesis, characterization and biological evaluation of Co(III) complexes with quinolone drugs.
yó P Abstract Nine novel cobalt(III) ternary complexes bearing 4N donor ligands (tris(2-aminoethyl)amine (tren) or tris(2-methylpyridyl)amine (tpa)) and (fluoro)quinolones (quinH) with antibacterial and potential antitumor activity have been synthesized, characterized and screened in various biological assays. The molecular structures of [Co(tpa)(nal)](PF6)2 (3) and [Co(tpa)(nor)(Co(tpa)(norH)](PF6)3(Cl)2∙5MeOH (8) (nal = deprotonated form of nalidixic acid, norH = norfloxacin) with the expected octahedral geometry and (O,O) coordination of the quinolone ligands are also reported. Cyclic voltammet...
Source: Journal of Inorganic Biochemistry - January 21, 2019 Category: Biochemistry Authors: Kozsup M, Farkas E, Bényei AC, Kasparkova J, Crlikova H, Brabec V, Buglyó P Tags: J Inorg Biochem Source Type: research

An in vitro study on dentin demineralization and remineralization: Collagen rearrangements and influence on the enucleated phase.
This study is aimed at gaining more insights on a molecular scale, through IR spectroscopy, into dentin demineralization and remineralization. The dentin demineralization by ethylenediaminetetraacetic acid, EDTA (17%, 2 h) significantly altered the secondary structure distribution of collagen, upon loss of interaction with calcium ions. To investigate dentin remineralization, previously demineralized human dentin slices were soaked in Dulbecco's Phosphate Buffered Saline (DPBS) or Hank's Balanced Salt Solution HBSS, in close contact with three commercial cements used as sustained releasing sources of Ca2+ and OH- ions (i...
Source: Journal of Inorganic Biochemistry - January 18, 2019 Category: Biochemistry Authors: Di Foggia M, Prati C, Gandolfi MG, Taddei P Tags: J Inorg Biochem Source Type: research

Mir-215-5p induces autophagy by targeting PI3K and activating ROS-mediated MAPK pathways in cardiomyocytes of chicken.
In this study, we report that miR-215-5p can promote myocardial autophagy by directly binding to the 3'untranslated region (3'UTR) of phosphatidylinositol-4, 5-bisphosphate 3-kinase (PI3K). Its target gene PI3K was confirmed by dual luciferase reporter assay, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot in cardiomyocytes. Our results showed that overexpression of miR-215-5p could trigger myocardial autophagy through PI3K-threonine-protein kinase (AKT)-target of rapamycin (TOR) pathway. Further studies revealed that autophagic cell death was dependent on the activation of extracellular signal-...
Source: Journal of Inorganic Biochemistry - January 18, 2019 Category: Biochemistry Authors: Cai J, Yang J, Liu Q, Gong Y, Zhang Y, Zheng Y, Yu D, Zhang Z Tags: J Inorg Biochem Source Type: research

Ru(II)/N-N/PPh3 complexes as potential anticancer agents against MDA-MB-231 cancer cells (N-N  = diimine or diamine).
Ru(II)/N-N/PPh3 complexes as potential anticancer agents against MDA-MB-231 cancer cells (N-N = diimine or diamine). J Inorg Biochem. 2019 Jan 18;193:70-83 Authors: Ribeiro GH, Colina-Vegas L, Clavijo JCT, Ellena J, Cominetti MR, Batista AA Abstract The rational design of anticancer agents that acts in specific biological targets is one of the most effective strategies for developing chemotherapeutic agents. Aiming at obtaining new ruthenium (II) compounds with good cytotoxicity against tumor cells, a series of new complexes of general formula [RuCl(PPh3)(Hdpa)(NN)]Cl [PPh3 = triphenylphosphin...
Source: Journal of Inorganic Biochemistry - January 18, 2019 Category: Biochemistry Authors: Ribeiro GH, Colina-Vegas L, Clavijo JCT, Ellena J, Cominetti MR, Batista AA Tags: J Inorg Biochem Source Type: research

Complexation of luteolin with lead (II): Spectroscopy characterization and theoretical researches.
Abstract The interactions of (CH3COO)2Pb·3H2O (lead acetate trihydrate) with luteolin, 5,7,3',4'-tetrahydroxyflavone, were investigated in methanol solution. The spectroscopy (UV-Vis, FT-IR, HPLC-MS, 1H NMR) and elemental analysis were adopted to assess the interaction of luteolin and Pb(II). The results show that luteolin reacts with Pb(II) through the chelating sites of 4‑carbonyl and 5-hydroxy in two luteolin molecules. The structures, energies, CDA (charge decomposition analysis) and orbitals analysis of the ligand and complex have been analyzed according to quantum-chemical calculation, which is furt...
Source: Journal of Inorganic Biochemistry - January 15, 2019 Category: Biochemistry Authors: Wang Q, Zhao L, Zhao H, Liu X, Gao L, Cheng N, Cao W Tags: J Inorg Biochem Source Type: research

Syntheses, crystal structures and biological evaluation of two new Cu(II) and Co(II) complexes based on (E)-2-(((4H-1,2,4-triazol-4-yl)imino)methyl)-6-methoxyphenol.
Abstract Two transition metal complexes of [M(TMP)2(H2O)2] (TMP-Cu, M = Cu; TMP-Co, M = Co) with (E)-2-(((4H-1,2,4-triazol-4-yl)imino)methyl)-6-methoxyphenol (H-TMP) were first synthesized and characterized by infrared analysis, elemental analysis and single crystal X-ray diffraction analysis. Notably, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay showed that TMP-Cu displayed relatively high cytotoxic activity against Hep-G2 cancer cells, and high selectivity between human hepatocellular carcinoma cells and normal HL-7702 cells, in comparison to TMP-Co and cisplatin. Further stud...
Source: Journal of Inorganic Biochemistry - January 15, 2019 Category: Biochemistry Authors: Zhang SM, Zhang HY, Qin QP, Fei JW, Zhang SH Tags: J Inorg Biochem Source Type: research

Photoinduced electron transfer within supramolecular hemoprotein co-assemblies and heterodimers containing Fe and Zn porphyrins.
This report describes a photoinduced electron transfer between Zn porphyrin and Fe porphyrin within a supramolecular cytochrome b562 (Cyt b562) co-assembly or heterodimer with a well-defined rigid structure formed by a metalloporphyrin-heme pocket interaction and a hydrogen-bond network at the protein interface. The photoinduced charge separation (CS: kCS = 320-600 s-1) and subsequent charge recombination (CR: kCR = 580-930 s-1) were observed in both the Cyt b562 co-assembly and the heterodimer. In contrast, interestingly, no ET events were observed in a system comprised of a flexible and structurally-undefined...
Source: Journal of Inorganic Biochemistry - January 14, 2019 Category: Biochemistry Authors: Kajihara R, Oohora K, Hayashi T Tags: J Inorg Biochem Source Type: research

Supramolecular redox-responsive substrate carrier activity of a ferrocenyl Janus device.
Abstract Supramolecular Janus compounds have recently attracted increasing attention owing to their dynamic reversible properties, distinct topological structures, and remarkable physicochemical characteristics, e.g., amphiphilicity, heterofunctionality, and high-density of terminal groups. Herein, a new redox-responsive supramolecular Janus device was designed and synthesized involving β-cyclodextrin and 2-fold ferrocene host-guest interactions. The complex formation was analyzed via one-dimensional 1H NMR and two-dimensional Nuclear Overhauser Enhancement Spectroscopy. FeCl3 and ascorbic acid were used as o...
Source: Journal of Inorganic Biochemistry - January 11, 2019 Category: Biochemistry Authors: Mu S, Ling Q, Liu X, Ruiz J, Astruc D, Gu H Tags: J Inorg Biochem Source Type: research

N ‑(6‑Aminohexyl)‑5‑chloro‑1‑naphthalenesulfonamide, a centrin antagonist, inhibits Tb3+/peptides-binding properties.
N‑(6‑Aminohexyl)‑5‑chloro‑1‑naphthalenesulfonamide, a centrin antagonist, inhibits Tb3+/peptides-binding properties. J Inorg Biochem. 2019 Jan 11;193:15-24 Authors: Li M, Zhang W, Yang B Abstract N‑(6‑Aminohexyl)‑5‑chloro‑1‑naphthalenesulfonamide (W-7), a kind of adjuvant chemotherapy, can bind to calmodulin and inhibit Ca2+/calmodulin-regulated enzyme activities and cell proliferation. Similar to calmodulin, euplotes octocarinatus centrin (EoCen) belongs to EF-hand superfamily of calcium-binding proteins. It is associated with nucleotide excision repair (NER), cell division cy...
Source: Journal of Inorganic Biochemistry - January 11, 2019 Category: Biochemistry Authors: Li M, Zhang W, Yang B Tags: J Inorg Biochem Source Type: research

Synthesis, anticancer activity and mechanism of iron chelator derived from 2,6-diacetylpyridine bis(acylhydrazones).
Abstract We synthesized five iron chelator derived from 2,6-diacetylpyridine bis(acylhydrazones) and proved their iron complexes structure by X-ray single crystal diffraction. These ligands have a significant anticancer proliferative activity and low cytotoxicity against normal cells. The Fe(III) complexes show reduced cytotoxic activity compared to the metal-free ligands. Anticancer mechanism studies indicate that ligands with a potential anticancer proliferation activity by inhibiting the activity of ribonucleotide reductase. Ligand rather than iron complexes regulate the expression of cell cycle associated prot...
Source: Journal of Inorganic Biochemistry - January 10, 2019 Category: Biochemistry Authors: Yao Q, Qi J, Zheng Y, Qian K, Wei L, Maimaitiyiming M, Cheng Z, Wang Y Tags: J Inorg Biochem Source Type: research

In vitro anti-bacterial and time kill evaluation of binuclear tricyclohexylphosphanesilver(I) dithiocarbamates, {Cy3PAg(S2CNRR')}2.
Abstract Four binuclear phosphanesilver(I) dithiocarbamates, {cyclohexyl3PAg(S2CNRR')}2 for R = R' = Et (1), CH2CH2 (2), CH2CH2OH (3) and R = Me, R' = CH2CH2OH (4) have been synthesised and characterised by spectroscopy and crystallography, and feature tri-connective, μ2-bridging dithiocarbamate ligands and distorted tetrahedral geometries based on PS3 donor sets. The compounds were evaluated for anti-bacterial activity against a total of 12 clinically important pathogens. Based on minimum inhibitory concentration (MIC) and cell viability tests (human embryonic kidney cells, HEK 293), 1-4 are sp...
Source: Journal of Inorganic Biochemistry - January 6, 2019 Category: Biochemistry Authors: Tan YJ, Tan YS, Yeo CI, Chew J, Tiekink ERT Tags: J Inorg Biochem Source Type: research

ZraP, the most prominent zinc protein under zinc stress conditions has no direct role in in-vivo zinc tolerance in Escherichia coli.
Abstract Escherichia coli ZraP (zinc resistance associated protein) is the major Zn containing soluble protein under Zn stress conditions. ZraP is the accessory protein of a bacterial two-component, Zn2+ sensitive signal transduction system ZraSR. ZraP has also been reported to act as a Zn2+ dependent molecular chaperone. An explanation why ZraP is the major Zn protein under the stress condition of Zn2+ overload (0.2 mM) has remained elusive. We have recombinantly produced E. coli ZraP and measured Zn2+ and Cu2+ affinity in-vitro using Isothermal Titration Calorimetry. ZraP has a significantly higher affinity fo...
Source: Journal of Inorganic Biochemistry - December 27, 2018 Category: Biochemistry Authors: van der Weel L, As KS, Dekker WJC, van den Eijnden L, van Helmond W, Schiphorst C, Hagen WR, Hagedoorn PL Tags: J Inorg Biochem Source Type: research

Copper complexes for biomedical applications: Structural insights, antioxidant activity and neuron compatibility.
This study was aimed to coordinate copper(II) with four small ligands (l-Serine, l-Histidine, Urea and Biuret) and to evaluate structural features, stability, antioxidant activity and neuronal compatibility of the resulting complexes. All complexes were synthesized with CuCl2 and purified by precipitation in alcohol. Elemental composition, X-rays diffraction and FTIR indicated that the complexes were in form of [Cu(ligand)2] and exhibited tridentate (l-Histidine), bidentate (l-Serine and Biuret) or monodentate (Urea) coordination with copper. UV-Vis absorbance profiles in physiologically relevant solutions and cyclic volta...
Source: Journal of Inorganic Biochemistry - December 26, 2018 Category: Biochemistry Authors: Esmaeili L, Perez MG, Jafari M, Paquin J, Ispas-Szabo P, Pop V, Andruh M, Byers J, Mateescu MA Tags: J Inorg Biochem Source Type: research

Trehalose prevents cadmium-induced hepatotoxicity by blocking Nrf2 pathway, restoring autophagy and inhibiting apoptosis.
In conclusion, these observations demonstrate that Tr treatment alleviates Cd-induced liver injury by blocking Nrf2 pathway, restoring autophagy and inhibiting apoptosis. PMID: 30599315 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - December 26, 2018 Category: Biochemistry Authors: Gong ZG, Wang XY, Wang JH, Fan RF, Wang L Tags: J Inorg Biochem Source Type: research

Half-sandwich iridium(III) complexes with α-picolinic acid frameworks and antitumor applications.
Half-sandwich iridium(III) complexes with α-picolinic acid frameworks and antitumor applications. J Inorg Biochem. 2018 Dec 24;192:52-61 Authors: Hao H, Liu X, Ge X, Zhao Y, Tian X, Ren T, Wang Y, Zhao C, Liu Z Abstract Eight half-sandwich iridiumIII (IrIII) complexes of the general formula [(η5-Cpxbiph)Ir(O^N)Cl] (Cpxbiph is tetramethyl(biphenyl)cyclopentadienyl, and the O^N is α-picolinic acid chelating ligand and its derivatives) were synthesized and characterized. Compared with cis-platin widely used in clinic, target IrIII complexes showed at most five times more potent antitumor ...
Source: Journal of Inorganic Biochemistry - December 24, 2018 Category: Biochemistry Authors: Hao H, Liu X, Ge X, Zhao Y, Tian X, Ren T, Wang Y, Zhao C, Liu Z Tags: J Inorg Biochem Source Type: research

Interaction of Cm(III) with human serum albumin studied by time-resolved laser fluorescence spectroscopy and NMR.
PJ Abstract The complexation of Cm(III) with human serum albumin (HSA) was investigated using time-resolved laser fluorescence spectroscopy (TRLFS). The Cm(III) HSA species is dominating the speciation between pH 7.0 and 9.3. The first coordination sphere is composed by three to four H2O molecules and five to six coordinating ligands from the protein. For the complex formation at pH 8.0 a conditional stability constant of logK = 6.16 ± 0.50 was determined. Furthermore, information on the Cm(III) HSA binding site were obtained. With increasing Cu(II) concentration the Cm(III) HSA complexation is...
Source: Journal of Inorganic Biochemistry - December 20, 2018 Category: Biochemistry Authors: Adam N, Adam C, Keskitalo M, Pfeuffer-Rooschüz J, Panak PJ Tags: J Inorg Biochem Source Type: research

Does phosphorylation increase the binding affinity of aluminum? A computational study on the aluminum interaction with serine and O-phosphoserine.
Abstract Several toxic effects arise from aluminum's presence in living systems, one of these effects is to alter the natural role of enzymes and non-enzyme proteins. Aluminum promotes the hyperphosphorylation of normal proteins. In order to assess the aluminum-binding abilities of phosphorylated proteins and peptides, the interaction of aluminum at different pH with serine and phosphoserine is studied by a Density Functional Theory study, combined with polarizable continuum models to account for bulk solvent effects, and the electronic structure of selected complexes are analyzed by Quantum Theory of "Atoms ...
Source: Journal of Inorganic Biochemistry - December 15, 2018 Category: Biochemistry Authors: Formoso E, Grande-Aztatzi R, Lopez X Tags: J Inorg Biochem Source Type: research

About the use of 13C-13C NOESY in bioinorganic chemistry.
Abstract Herein we present examples of the application of the 13C-13C Nuclear Overhauser Effect Spectroscopy (NOESY) experiment to the study of metalloproteins and we critically discuss the advantages and drawbacks of the method as a function of the molecular size of the investigated systems. The contribution is focused on a few case studies among the systems analyzed in the group of the corresponding author. The 13C-13C NOESY experiment represents the gold standard for the observation of NMR signals in the 480 kDa ferritin nanocage and for monitoring its interaction with iron. By decreasing the protein size, th...
Source: Journal of Inorganic Biochemistry - December 8, 2018 Category: Biochemistry Authors: Ghini V, Chevance S, Turano P Tags: J Inorg Biochem Source Type: research

Synthesis, characterization, DNA binding, topoisomerase I inhibition and antiproliferation activities of three new functionalized terpyridine platinum(II) complexes.
Abstract Three new platinum(II) complexes with pendent morpholine were synthesized, namely complex 1 ([Pt(L)Cl]CF3SO3), complex 2 ([Pt(L)(NH3)](CF3SO3)2) and complex 3 ([Pt(L)(PPh3)](CF3SO3)2), where L = 4'-[4-(4-morpholinobutyloxy)phenyl]-2,2':6',2″-terpyridine and PPh3 = triphenylphosphine. The detailed molecular structures of complex 3, L and its precursor L' (1,4'-[4-(4-bromobutyloxy)phenyl]-2,2':6',2″-terpyridine) were determined by single crystal X-ray diffraction. An evaluation of in vitro cytotoxicity for both ligand and complexes was performed by methyl thiazolyl tetrazolium (MTT) assa...
Source: Journal of Inorganic Biochemistry - December 7, 2018 Category: Biochemistry Authors: Chai K, Kuang W, Lan Z, Zhang L, Jiang Y, Han T, Niu J, Wang J, Duan X Tags: J Inorg Biochem Source Type: research

Synthesis, photodynamic activities, and cytotoxicity of new water-soluble cationic gallium(III) and zinc(II) phthalocyanines.
Abstract The cationic Ga(III) and Zn(II) phthalocyanines carrying N-methyl-pyridinium groups at eight peripheral β-positionshave been synthesized. These complexes are highly soluble in dimethyl sulfoxide (DMSO) and moderately soluble in water and phosphate buffered saline (PBS); both Ga(III)Cl and Zn(II) complexes have shown no aggregation in water up to 1.2 × 10-4 and 1.5 × 10-5 M, respectively. A higher water-solubility of Ga(III)Cl complex as compared to Zn(II) complex is ascribed to the presence of an axially coordinated chloride. The spectroscopic properties, photogeneration of sing...
Source: Journal of Inorganic Biochemistry - December 5, 2018 Category: Biochemistry Authors: Fujishiro R, Sonoyama H, Ide Y, Fujimura T, Sasai R, Nagai A, Mori S, Kaufman NEM, Zhou Z, Vicente MGH, Ikeue T Tags: J Inorg Biochem Source Type: research

A series of two-photon absorption organotin (IV) cyano carboxylate derivatives for targeting nuclear and visualization of anticancer activities.
Abstract Compared to organotin (IV) compounds with biochemical activity, two-photon absorption (2PA) organotin (IV) complexes for targeting nuclear with anticancer activities are rarely reported. Here, two novel 2PA organotin (IV) cyano carboxylate complexes (C1Sn-1, C1Sn-2) are synthesized and characterized. The two-photon absorption cross section values (δ) in the near-infrared region are significantly enhanced for C1Sn-2 compared to C1Sn-1, thus developing for targeting nuclear by two-photon fluorescence microscopy (2PFM). C1Sn-2 could specifically target nuclear DNA in vitro. The mechanism demonstrated t...
Source: Journal of Inorganic Biochemistry - December 5, 2018 Category: Biochemistry Authors: Zhang Q, Zhang M, Wang H, Tian X, Ma W, Luo L, Wu J, Zhou H, Li S, Tian Y Tags: J Inorg Biochem Source Type: research

In-vitro evaluation studies of 7-chloro-4-aminoquinoline Schiff bases and their copper complexes as cholinesterase inhibitors.
as MD Abstract Alzheimer's disease (AD) is one of the most common age-related neurodegenerative disorders. Aggregation of amyloid-β peptide into extracellular plaques with incorporation of metal ions, such as Cu2+, and reduction of the neurotransmitter acetylcholine levels are among the factors associated to the AD brain. Hence, a series of 7-chloro-4-aminoquinoline Schiff bases (HLa-e) were synthesized and their cytotoxicity and anti-cholinesterase activity, assessed for Alzheimer's disease. The intrinsic relationship between Cu2+ and the amyloidogenic plaques encouraged us to investigate the chelating abili...
Source: Journal of Inorganic Biochemistry - November 30, 2018 Category: Biochemistry Authors: Zanon VS, Lima JA, Cuya T, Lima FRS, da Fonseca ACC, Gomez JG, Ribeiro RR, França TCC, Vargas MD Tags: J Inorg Biochem Source Type: research

Structure-activity relationships of 2 ‑quinolinecarboxaldehyde thiosemicarbazone gallium(III) complexes with potent and selective anticancer activity.
Structure-activity relationships of 2‑quinolinecarboxaldehyde thiosemicarbazone gallium(III) complexes with potent and selective anticancer activity. J Inorg Biochem. 2018 Nov 28;191:174-182 Authors: Cao W, Qi J, Qian K, Tian L, Cheng Z, Wang Y Abstract Six gallium(III) complexes (Ga1-Ga6) with 2‑quinolinecarboxaldehyde thiosemicarbazone analogues were synthesized and characterized. These gallium(III) complexes exhibited potent anticancer activity and exceeded that of the corresponding metal free ligands. Importantly, these gallium(III) complexes have a strong selectivity for tumor cells. Through ...
Source: Journal of Inorganic Biochemistry - November 28, 2018 Category: Biochemistry Authors: Cao W, Qi J, Qian K, Tian L, Cheng Z, Wang Y Tags: J Inorg Biochem Source Type: research