Specific binding modes of Cu(I) and Ag(I) with neurotoxic domain of the human prion protein.
Abstract Prion diseases are neurodegenerative disorders associated with a conformational change of the normal cellular isoform of the prion protein (PrP(C)) to an abnormal scrapie isoform (PrP(Sc)). human prion protein (hPrP(C)) is able to bind up to six Cu(II) ions. Four of them are distributed in the octarepeat domain, containing four tandem-repetitions of the sequence PHGGGWGQ. Immediately outside the octarepeat domain, in so called PrP amyloidogenic region, two additional and independent Cu(II) binding sites, encompassing His96 and His111 residues, respectively, are present. Considering the potential involveme...
Source: Journal of Inorganic Biochemistry - November 11, 2015 Category: Biochemistry Authors: Valensin D, Padula EM, Hecel A, Luczkowski M, Kozlowski H Tags: J Inorg Biochem Source Type: research

Pd(2+)-mediated base pairing in oligonucleotides.
g T Abstract Two short glycol nucleic acid (GNA) oligonucleotides, having either a terminal or an intrachain nucleobase replaced by the pyridine-2,6-dicarboxamide chelate of Pd(2+), have been synthesized and their hybridization properties studied by melting temperature measurements. In the termini of a double-stranded oligonucleotide, the Pd(2+) chelates provided dramatic stabilization of the duplex relative to its metal-free counterpart, in all likelihood owing to formation of Pd(2+)-mediated base pairs between pyridine-2,6-dicarboxamide and the opposing nucleobase. In contrast, no stabilization was observed when...
Source: Journal of Inorganic Biochemistry - November 11, 2015 Category: Biochemistry Authors: Golubev O, Turc G, Lönnberg T Tags: J Inorg Biochem Source Type: research

Interactions of two cytotoxic organoruthenium(II) complexes with G-quadruplex.
J, Turel I Abstract Two previously isolated cytotoxic complexes [(η(6)-p-cymene)Ru(κ(2)-CF3COCHCOC5H3O)L](n+) (L=Cl (1); n=0, pta (2) (pta=1,3,5-triaza-7-phosphaadamantane); n=1) were investigated for their selectivity and ability to interact with DNA G-quadruplex adopted by d[G3ATG3ACACAG4ACG3] (3) whose topology exhibits diagonal, edge-type and double-chain reversal loops. Structural changes were followed using high-resolution NMR techniques in the presence of 1 and 2. Results showed weak interaction between the organoruthenium complexes and G-quadruplex. Moreover, no significant changes in thermal st...
Source: Journal of Inorganic Biochemistry - November 11, 2015 Category: Biochemistry Authors: Seršen S, Šket P, Plavec J, Turel I Tags: J Inorg Biochem Source Type: research

Cytochrome unfolding pathways from computational analysis of crystal structures.
ez RA Abstract We have developed a model to study the role of geometrical factors in influencing the early stages of unfolding in three cytochromes: cyt c', cyt c-b562 and cyt c. Each stage in unfolding is quantified by the spatial extension λ̂i of n-residue segments, and by their angular extension 〈βn〉. Similarities and differences between and among the three cytochromes in the unfolding of helical and non-helical regions can be determined by analyzing the data for each signature separately. Definite conclusions can be drawn when spatial and angular changes are considered in tandem. To facilitate...
Source: Journal of Inorganic Biochemistry - November 10, 2015 Category: Biochemistry Authors: Kozak JJ, Gray HB, Garza-López RA Tags: J Inorg Biochem Source Type: research

An alternative reaction for heme degradation catalyzed by the Escherichia coli O157:H7 ChuS protein: Release of hematinic acid, tripyrrole and Fe(III).
, Couture M Abstract As part of the machinery to acquire, internalize and utilize heme as a source of iron from the host, some bacteria possess a canonical heme oxygenase, where heme plays the dual role of substrate and cofactor, the later catalyzing the cleavage of the heme moiety using O2 and electrons, and resulting in biliverdin, carbon monoxide and ferrous non-heme iron. We have previously reported that the Escherichia coli O157:H7 ChuS protein, which is not homologous to heme oxygenases, can bind and degrade heme in a reaction that releases carbon monoxide. Here, we have pursued a detailed characterization o...
Source: Journal of Inorganic Biochemistry - November 10, 2015 Category: Biochemistry Authors: Ouellet YH, Ndiaye CT, Gagné SM, Sebilo A, Suits MD, Jubinville É, Jia Z, Ivancich A, Couture M Tags: J Inorg Biochem Source Type: research

Kinetic and structural studies reveal a unique binding mode of sulfite to the nickel center in urease.
Abstract Urease is the most efficient enzyme known to date, and catalyzes the hydrolysis of urea using two Ni(II) ions in the active site. Urease is a virulence factor in several human pathogens, while causing severe environmental and agronomic problems. Sporosarcina pasteurii urease has been used extensively in the structural characterization of the enzyme. Sodium sulfite has been widely used as a preservative in urease solutions to prevent oxygen-induced oxidation, but its role as an inhibitor has also been suggested. In the present study, isothermal titration microcalorimetry was used to establish sulfite as a ...
Source: Journal of Inorganic Biochemistry - November 5, 2015 Category: Biochemistry Authors: Mazzei L, Cianci M, Benini S, Bertini L, Musiani F, Ciurli S Tags: J Inorg Biochem Source Type: research

In vitro effects of bis(1,2-dimethyl-3-hydroxy-4-pyridinonato)oxidovanadium(IV), or VO(dmpp)2, on insulin secretion in pancreatic islets of type 2 diabetic Goto-Kakizaki rats.
CG PMID: 26559485 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - November 4, 2015 Category: Biochemistry Authors: Pelletier J, Domingues N, Castro MM, Östenson CG Tags: J Inorg Biochem Source Type: research

Synthesis of biocompatible polymeric nano-capsules based on calcium carbonate: A potential cisplatin delivery system.
PMID: 26560986 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - November 1, 2015 Category: Biochemistry Authors: Vergaro V, Papadia P, Leporatti S, De Pascali SA, Fanizzi FP, Ciccarella G Tags: J Inorg Biochem Source Type: research

Modulating the nitrite reductase activity of globins by varying the heme substituents: Utilizing myoglobin as a model system.
Abstract Globins, such as hemoglobin (Hb) and myoglobin (Mb), have gained attention for their ability to reduce nitrite (NO2(-)) to nitric oxide (NO). The molecular interactions that regulate this chemistry are not fully elucidated, therefore we address this issue by investigating one part of the active site that may control this reaction. Here, the effects of the 2,4-heme substituents on the nitrite reductase (NiR) reaction, and on the structures and energies of the ferrous nitrite intermediates, are investigated using Mb as a model system. This is accomplished by studying Mbs with hemes that have different 2,4-R...
Source: Journal of Inorganic Biochemistry - October 22, 2015 Category: Biochemistry Authors: Galinato MG, Fogle RS, Stetz A, Galan JF Tags: J Inorg Biochem Source Type: research

Detoxification of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) by cytochrome P450 enzymes: A theoretical investigation.
Abstract Two types of detoxification routes, N-demethylation to form 4-phenyl-1,2,3,6-tetrahydropyridine (PTP) and aromatic hydroxylation to generate 4-(4'-hydroxyphenyl)-1-methyl-1,2,3,6-tetrahydropyridine (MPTP-OH), for 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mediated by Compound I (Cpd I) of cytochrome P450 are investigated theoretically using hybrid density functional calculations. Quantum chemical results reveal that for the N-demethylation, the initial C-H bond activation is achieved via a hydrogen atom transfer (HAT) mechanism. This is followed by a subsequent O-rebound to yield the carbinolamin...
Source: Journal of Inorganic Biochemistry - October 22, 2015 Category: Biochemistry Authors: Li XX, Wang Y, Zheng QC, Zhang HX Tags: J Inorg Biochem Source Type: research

Structure-activity relationship studies on rhodamine B-based fluorogenic probes and their activation by anticancer platinum(II) compounds.
In this report, we seek to establish the mechanism of activation of Rho-DDTC through a structure-activity relationship study on its structural analogues. PMID: 26518138 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - October 22, 2015 Category: Biochemistry Authors: Ong JX, Yap JY, Yap SQ, Ang WH Tags: J Inorg Biochem Source Type: research

pH dependence of cyanide and imidazole binding to the heme domains of Sinorhizobium meliloti and Bradyrhizobium japonicum FixL.
Abstract Equilibrium and kinetic properties of cyanide and imidazole binding to the heme domains of Sinorhizobium meliloti and Bradyrhizobium japonicum FixL (SmFixLH and BjFixLH) have been investigated between pH5 and 11. KD determinations were made at integral pH values, with the strongest binding at pH9 for both ligands. KD for the cyanide complexes of BjFixLH and SmFixLH is 0.15±0.09 and 0.50±0.20μM, respectively, and 0.70±0.01mM for imido-BjFixLH. The association rate constants are pH dependent with maximum values of 443±8 and 252±61M(-1)s(-1) for cyano complexes of BjFixL...
Source: Journal of Inorganic Biochemistry - October 21, 2015 Category: Biochemistry Authors: Bidwai AK, Ahrendt AJ, Sullivan JS, Vitello LB, Erman JE Tags: J Inorg Biochem Source Type: research

Differences in protein binding and excretion of Triapine and its Fe(III) complex.
Abstract Triapine has been investigated as anticancer drug in multiple clinical phase I/II trials. Although promising anti-leukemic activity was observed, Triapine was ineffective against solid tumors. The reasons are currently widely unknown. The biological activity of Triapine is strongly connected to its iron complex (Fe-Triapine) which is pharmacologically not investigated. Here, novel analytical tools for Triapine and Fe-Triapine were developed and applied for cell extracts and body fluids of treated mice. Triapine and its iron complex showed a completely different behavior: for Triapine, low protein binding ...
Source: Journal of Inorganic Biochemistry - October 19, 2015 Category: Biochemistry Authors: Pelivan K, Miklos W, van Schoonhoven S, Koellensperger G, Gille L, Berger W, Heffeter P, Kowol CR, Keppler BK Tags: J Inorg Biochem Source Type: research

Aggregates of a cationic porphyrin as supramolecular probes for biopolymers.
PMID: 26490712 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - October 9, 2015 Category: Biochemistry Authors: Occhiuto IG, Samperi M, Trapani M, De Luca G, Romeo A, Pasternack RF, Scolaro LM Tags: J Inorg Biochem Source Type: research

Spectroscopic studies, DFT calculations, and cytotoxic activity of novel silver(I) complexes of hydroxy ortho-substituted-nitro-2H-chromen-2-one ligands and a phenanthroline adduct.
Abstract Silver(I) complexes of coumarin-based ligands and one of their phenanthroline (phen) adducts have been prepared and characterized using microanalytical data, molar conductivity, IR, (1)H and (13)C NMR, UV-Vis, and atomic absorption (AAS) spectroscopies. The binding modes of the coumarin-based ligands and the most probable structure of their Ag(I) complexes were predicted by means of molecular modeling and calculations of their IR, NMR, and absorption spectra using density functional theory (DFT). The cytotoxicity of the compounds studied against human-derived hepatic carcinoma cells (Hep-G2) and a renal c...
Source: Journal of Inorganic Biochemistry - October 9, 2015 Category: Biochemistry Authors: Mujahid M, Kia AF, Duff B, Egan DA, Devereux M, McClean S, Walsh M, Trendafilova N, Georgieva I, Creaven BS Tags: J Inorg Biochem Source Type: research

Modeling the chelation of As(III) in lewisite by dithiols using density functional theory and solvent-assisted proton exchange.
Abstract Dithiols such as British anti-lewisite (BAL, rac-2,3-dimercaptopropanol) are an important class of antidotes for the blister agent lewisite (trans-2-chlorovinyldichloroarsine) and, more generally, are chelating agents for arsenic and other toxic metals. The reaction of the vicinal thiols of BAL with lewisite through the chelation of the As(III) center has been modeled using density functional theory (DFT) and solvent-assisted proton exchange (SAPE), a microsolvation method that uses a network of water molecules to mimic the role of bulk solvent in models of aqueous phase chemical reactions. The small acti...
Source: Journal of Inorganic Biochemistry - October 9, 2015 Category: Biochemistry Authors: Harper LK, Bayse CA Tags: J Inorg Biochem Source Type: research

Induction of apoptosis in leukemia cell lines by new copper(II) complexes containing naphthyl groups via interaction with death receptors.
Abstract The synthesis, physico-chemical characterization and cytotoxicity of four new ligands and their respective copper(II) complexes toward two human leukemia cell lines (THP-1 and U937) are reported (i.e. [(HL1)Cu(μ-Cl)2Cu(HL1)]Cl2·H2O (1), [(H2L2)Cu(μ-Cl)2Cu(H2L2)]Cl2·5H2O (2), [(HL3)Cu(μ-Cl)2Cu(HL3)]Cl2·4H2O (3), [(H2L4)Cu(μ-Cl)2Cu(H2L4)]Cl2·6H2O (4)). Ligands HL1 and HL3 contain two pyridines, amine and alcohol moieties with a naphthyl pendant unit yielding a N3O coordination metal environment. Ligands H2L2 and H2L4 have pyridine, phenol, amine and alcohol groups wit...
Source: Journal of Inorganic Biochemistry - October 3, 2015 Category: Biochemistry Authors: Fernandes C, Horn A, Lopes BF, Bull ES, Azeredo NF, Kanashiro MM, Borges FV, Bortoluzzi AJ, Szpoganicz B, Pires AB, Franco RW, Almeida JC, Maciel LL, Resende JA, Schenk G Tags: J Inorg Biochem Source Type: research

Development of the aza-crown ether metal complexes as artificial hydrolase.
Abstract Hydrolases play a crucial role in the biochemical process, which can catalyze the hydrolysis of various compounds like carboxylic esters, phosphoesters, amides, nucleic acids, peptides, and so on. The design of artificial hydrolases has attracted extensive attention due to their scientific significance and potential applications in the field of gene medicine and molecular biology. Numerous macrocyclic metal complexes have been used as artificial hydrolase in the catalytic hydrolysis of the organic substrate. Aza-crown ether for this comment is a special class of the macrocyclic ligand containing both the ...
Source: Journal of Inorganic Biochemistry - October 3, 2015 Category: Biochemistry Authors: Yu L, Li FZ, Wu JY, Xie JQ, Li S Tags: J Inorg Biochem Source Type: research

Zinc pyrithione inhibits caspase-3 activity, promotes ErbB1-ErbB2 heterodimerization and suppresses ErbB2 downregulation in cardiomyocytes subjected to ischemia/reperfusion.
Abstract Heart tissue becomes zinc-depleted and the capacity to mobilize labile zinc is diminished, indicating zinc dyshomeostasis during ischemia/reperfusion (I/R). Apparently, zinc pyrithione restores the basal zinc levels during I/R and prevents apoptosis by activating phosphatidyl inositol-3-kinase/Akt and targeting mitochondrial permeability transition. Receptor tyrosine kinases of the ErbB family (ErbB1 to ErbB4) are cell surface proteins that can regulate cell growth, proliferation and survival. Previous studies have shown that zinc pyrithione-induced activation of PI3kinase/Akt requires ErbB2 expression. O...
Source: Journal of Inorganic Biochemistry - September 26, 2015 Category: Biochemistry Authors: Bodiga VL, Thokala S, Vemuri PK, Bodiga S Tags: J Inorg Biochem Source Type: research

Aluminum induces inflammatory and proteolytic alterations in human monocytic cell line.
Abstract The increasing exposure to aluminum has been linked with the development of different human pathologies (e.g., breast cancer, myofasciitis, neurodegenerative diseases), probably due to the consistent presence of aluminum salts in widely diffused cosmetic products and vaccines. However, the mechanisms underlying immunologic and proliferative alterations still remain unknown. In the present study we investigated the ability of different aluminum compounds (i.e., aluminum chloride vs Imject® Alum, a mixture of aluminum and magnesium hydroxide) to trigger both inflammatory and proteolytic responses in U-9...
Source: Journal of Inorganic Biochemistry - September 21, 2015 Category: Biochemistry Authors: Ligi D, Santi M, Croce L, Mannello F Tags: J Inorg Biochem Source Type: research

Efficacy of chelation therapy to remove aluminium intoxication.
PMID: 26404567 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - September 21, 2015 Category: Biochemistry Authors: Fulgenzi A, De Giuseppe R, Bamonti F, Vietti D, Ferrero ME Tags: J Inorg Biochem Source Type: research

Aluminum and iron can be deposited in the calcified matrix of bone exostoses.
PMID: 26404568 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - September 16, 2015 Category: Biochemistry Authors: Chappard D, Mabilleau G, Moukoko D, Henric N, Steiger V, Le Nay P, Frin JM, De Bodman C Tags: J Inorg Biochem Source Type: research

Importance of selenium and selenoprotein for brain function: From antioxidant protection to neuronal signalling.
Abstract Multiple biological functions of selenium manifest themselves mainly via 25 selenoproteins that have selenocysteine at their active centre. Selenium is vital for the brain and seems to participate in the pathology of disorders such as Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis and epilepsy. Since selenium was shown to be involved in diverse functions of the central nervous system, such as motor performance, coordination, memory and cognition, a possible role of selenium and selenoproteins in brain signalling pathways may be assumed. The aim of the present review is to analyse ...
Source: Journal of Inorganic Biochemistry - September 15, 2015 Category: Biochemistry Authors: Solovyev ND Tags: J Inorg Biochem Source Type: research

Effects of gold complexes on the assembly behavior of human islet amyloid polypeptide.
This study evaluated the inhibitory effects of three gold complexes with different nitrogen-containing aromatic ligands, namely, [Au(bipy)Cl2][PF6] (1), [Au(Ph2bpy)Cl2]Cl (2), and [Au(phen)Cl2]Cl (3), on the amyloid fibrillization of hIAPP. The complexes interacted with the peptide mainly through hydrophobic interaction and metal coordination. The concentration dependence of hIAPP aggregation on gold complex indicated that the assembly behavior of hIAPP is significantly affected by these compounds. The gold complexes inhibited peptide aggregation through dimerization and stabilized the peptide to monomers. Gold ion was fou...
Source: Journal of Inorganic Biochemistry - September 15, 2015 Category: Biochemistry Authors: He L, Zhu D, Zhao C, Jia X, Wang X, Du W Tags: J Inorg Biochem Source Type: research

Effect of aluminium on migration of oestrogen unresponsive MDA-MB-231 human breast cancer cells in culture.
Abstract Aluminium (Al) has been measured in human breast tissue, and may be a contributory factor in breast cancer development. At the 10th Keele meeting, we reported that long-term exposure to Al could increase migratory properties of oestrogen-responsive MCF-7 human breast cancer cells suggesting a role for Al in the metastatic process. We now report that long-term exposure (20-25weeks) to Al chloride or Al chlorohydrate at 10(-4)M or 10(-5)M concentrations can also increase the migration of oestrogen unresponsive MDA-MB-231 human breast cancer cells as measured using time-lapse microscopy and xCELLigence techn...
Source: Journal of Inorganic Biochemistry - September 8, 2015 Category: Biochemistry Authors: Bakir A, Darbre PD Tags: J Inorg Biochem Source Type: research

Molar absorption coefficients and stability constants of metal complexes of 4-(2-pyridylazo)resorcinol (PAR): Revisiting common chelating probe for the study of metalloproteins.
Abstract 4-(2-Pyridylazo)resorcinol (PAR) is one of the most popular chromogenic chelator used in the determination of the concentrations of various metal ions from the d, p and f blocks and their affinities for metal ion-binding biomolecules. The most important characteristics of such a sensor are the molar absorption coefficient and the metal-ligand complex dissociation constant. However, it must be remembered that these values are dependent on the specific experimental conditions (e.g. pH, solvent components, and reactant ratios). If one uses these values to process data obtained in different conditions, the fi...
Source: Journal of Inorganic Biochemistry - September 5, 2015 Category: Biochemistry Authors: Kocyła A, Pomorski A, Krężel A Tags: J Inorg Biochem Source Type: research

Assessing the contribution of the two protein disulfide isomerases PDIA1 and PDIA3 to cisplatin resistance.
Abstract Intracellular binding of cisplatin to non-DNA partners, such as proteins, has received increasing attention as an additional mode of action and as mechanism of resistance. We investigated two cisplatin-interacting isoforms of protein disulfide isomerase regarding their contribution to acquired cisplatin resistance using sensitive and resistant A2780/A2780cis ovarian cancer cells. Cisplatin cytotoxicity was assessed after knockdown of either protein disulfide isomerase family A member 1 (PDIA1) or protein disulfide isomerase family A member 3 (PDIA3). Whereas PDIA1 knockdown led to increased cytotoxicity i...
Source: Journal of Inorganic Biochemistry - September 5, 2015 Category: Biochemistry Authors: Kullmann M, Kalayda GV, Hellwig M, Kotz S, Hilger RA, Metzger S, Jaehde U Tags: J Inorg Biochem Source Type: research

Comparative solution equilibrium studies on pentamethylcyclopentadienyl rhodium complexes of 2,2'-bipyridine and ethylenediamine and their interaction with human serum albumin.
r A, Keppler BK, Kandioller W PMID: 26364131 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - September 2, 2015 Category: Biochemistry Authors: Enyedy ÉA, Mészáros JP, Dömötör O, Hackl CM, Roller A, Keppler BK, Kandioller W Tags: J Inorg Biochem Source Type: research

Vanadium(IV/V) complexes of Triapine and related thiosemicarbazones: Synthesis, solution equilibrium and bioactivity.
ute;A Abstract The stoichiometry and thermodynamic stability of vanadium(IV/V) complexes of Triapine and two related α(N)-heterocyclic thiosemicarbazones (TSCs) with potential antitumor activity have been determined by pH-potentiometry, EPR and (51)V NMR spectroscopy in 30% (w/w) dimethyl sulfoxide/water solvent mixtures. In all cases, mono-ligand complexes in different protonation states were identified. Dimethylation of the terminal amino group resulted in the formation of vanadium(IV/V) complexes with considerably higher stability. Three of the most stable complexes were also synthesized in solid state an...
Source: Journal of Inorganic Biochemistry - September 1, 2015 Category: Biochemistry Authors: Kowol CR, Nagy NV, Jakusch T, Roller A, Heffeter P, Keppler BK, Enyedy ÉA Tags: J Inorg Biochem Source Type: research

Aluminum and its effect in the equilibrium between folded/unfolded conformation of NADH.
Abstract Nicotinamide adenine dinucleotide (NADH) is one of the most abundant cofactor employed by proteins and enzymes. The molecule is formed by two nucleotides that can lead to two main conformations: folded/closed and unfolded/open. Experimentally, it has been determined that the closed form is about 2kcal/mol more stable than the open formed. Computationally, a correct description of the NADH unfolding process is challenging due to different reasons: 1) The unfolding process shows a very low energy difference between the two conformations 2) The molecule can form a high number of internal hydrogen bond intera...
Source: Journal of Inorganic Biochemistry - August 29, 2015 Category: Biochemistry Authors: Formoso E, Mujika JI, Grabowski SJ, Lopez X Tags: J Inorg Biochem Source Type: research

Cationic Pd(II)/Pt(II) 5,5-diethylbarbiturate complexes with bis(2-pyridylmethyl)amine and terpyridine: Synthesis, structures,DNA/BSA interactions, intracellular distribution, cytotoxic activity and induction of apoptosis.
PMID: 26339715 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - August 29, 2015 Category: Biochemistry Authors: Icsel C, Yilmaz VT, Kaya Y, Durmus S, Sarimahmut M, Buyukgungor O, Ulukaya E Tags: J Inorg Biochem Source Type: research

Differential activation of genes related to aluminium tolerance in two contrasting rice cultivars.
In conclusion, the Al-exclusion strategy of Nipponbare, at least in part mediated by STAR1 and probably regulated by ABA, provided better protection against Al toxicity than the accumulation and internal detoxification strategy of Modan mediated by Nrat1, ALS1 and ARS1. PMID: 26337117 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - August 28, 2015 Category: Biochemistry Authors: Roselló M, Poschenrieder C, Gunsé B, Barceló J, Llugany M Tags: J Inorg Biochem Source Type: research

Identification of differential anti-neoplastic activity of copper bis(thiosemicarbazones) that is mediated by intracellular reactive oxygen species generation and lysosomal membrane permeabilization.
We examined the structure-activity relationships of twelve bis(thiosemicarbazones) to elucidate factors regarding their anti-cancer efficacy. Importantly, the alkyl substitutions at the diimine position of the ligand backbone resulted in two distinct groups, namely, unsubstituted/monosubstituted and disubstituted bis(thiosemicarbazones). This alkyl substitution pattern governed their: (1) Cu(II/I) redox potentials; (2) ability to induce cellular (64)Cu release; (3) lipophilicity; and (4) anti-proliferative activity. The potent anti-cancer Cu complex of the unsubstituted bis(thiosemicarbazone) analog, glyoxal bis(4-methyl-3...
Source: Journal of Inorganic Biochemistry - August 17, 2015 Category: Biochemistry Authors: Stefani C, Al-Eisawi Z, Jansson PJ, Kalinowski DS, Richardson DR Tags: J Inorg Biochem Source Type: research

The adsorption of allergoids and 3-O-desacyl-4'-monophosphoryl lipid A (MPL®) to microcrystalline tyrosine (MCT) in formulations for use in allergy immunotherapy.
The adsorption of allergoids and 3-O-desacyl-4'-monophosphoryl lipid A (MPL®) to microcrystalline tyrosine (MCT) in formulations for use in allergy immunotherapy. J Inorg Biochem. 2015 Aug 15; Authors: Bell AJ, Heath MD, Hewings SJ, Skinner MA Abstract Infectious disease vaccine potency is affected by antigen adjuvant adsorption. WHO and EMA guidelines recommend limits and experimental monitoring of adsorption in vaccines and allergy immunotherapies. Adsorbed allergoids and MPL® in MATA-MPL allergy immunotherapy formulations effectively treat IgE mitigated allergy. Understanding vaccine antige...
Source: Journal of Inorganic Biochemistry - August 15, 2015 Category: Biochemistry Authors: Bell AJ, Heath MD, Hewings SJ, Skinner MA Tags: J Inorg Biochem Source Type: research

Effects of aluminium chloride and aluminium chlorohydrate on DNA repair in MCF10A immortalised non-transformed human breast epithelial cells.
Abstract Use of underarm aluminium (Al)-based antiperspirant salts may be a contributory factor in breast cancer development. At the 10th Keele meeting, Al was reported to cause anchorage-independent growth and double strand DNA breaks in MCF10A immortalised non-transformed human breast epithelial cells. We now report that exposure of MCF10A cells to Al chloride or Al chlorohydrate also compromised DNA repair systems. Long-term (19-21weeks) exposure to Al chloride or Al chlorohydrate at a 10(-4)M concentration resulted in reduced levels of BRCA1 mRNA as determined by real-time RT-PCR and BRCA1 protein as determine...
Source: Journal of Inorganic Biochemistry - August 15, 2015 Category: Biochemistry Authors: Farasani A, Darbre PD Tags: J Inorg Biochem Source Type: research

Cytotoxic activity, DNA damage, cellular uptake, apoptosis and western blot analysis of ruthenium(II) polypyridyl complex against human lung decarcinoma A549 cell.
Abstract A new ruthenium(II) polypyridyl complex [Ru(dmp)2(pddppn)](ClO4)2Ru1 was synthesized and characterized. The cytotoxic activity in vitro of the complex was evaluated by MTT method. Ru1 shows high effect on the inhibition of the cell growth against BEL-7402, HeLa, MG-63 and A549 cells with low IC50 values of 1.6±0.4, 9.0±0.8, 1.5±0.2 and 1.5±0.3μM, respectively. The cellular uptake indicates that Ru1 can enter into the cytoplasm and accumulate in the cell nuclei. Ru1 can induce apoptosis in A549 cells and enhance the levels of reactive oxygen species (ROS) and induce the decre...
Source: Journal of Inorganic Biochemistry - August 12, 2015 Category: Biochemistry Authors: Lai SH, Jiang GB, Yao JH, Li W, Han BJ, Zhang C, Zeng CC, Liu YJ Tags: J Inorg Biochem Source Type: research

Zinc(II) and copper(II) complexes with hydroxypyrone iron chelators.
ute;lez-Pérez JM Abstract High stability of the complexes formed at physiological pH is one of the basic requisites that a good iron chelator must possess. At the same time the chelating agent must be selective toward iron, i.e., the stability of iron complexes must be significantly higher than that of the complexes formed with essential metal ions, in order that these last ones do not perturb iron chelation. In the frame of our research on iron chelators we have designed and synthesized a series of tetradentate derivatives of kojic acid, and examined their binding properties toward Fe(3+) and Al(3+). In th...
Source: Journal of Inorganic Biochemistry - August 11, 2015 Category: Biochemistry Authors: Lachowicz JI, Nurchi VM, Crisponi G, Jaraquemada-Pelaez MG, Ostrowska M, Jezierska J, Gumienna-Kontecka E, Peana M, Zoroddu MA, Choquesillo-Lazarte D, Niclós-Gutiérrez J, González-Pérez JM Tags: J Inorg Biochem Source Type: research

Redox-implications associated with the formation of complexes between copper ions and reduced or oxidized glutathione.
, Speisky H Abstract Binding of copper by reduced glutathione (GSH) is generally seen as a mechanism to lower, if not abolish, the otherwise high electrophilicity and redox activity of its free ions. In recent years, however, this concept has been contradicted by new evidence revealing that, rather than stabilizing free copper ions, its binding to GSH leads to the formation of a Cu(I)-[GSH]2 complex capable of reducing molecular oxygen into superoxide. It is now understood that, under conditions leading to the removal of such radicals, the Cu(I)-[GSH]2 complex is readily oxidized into Cu(II)-GSSG. Interestingly, i...
Source: Journal of Inorganic Biochemistry - August 11, 2015 Category: Biochemistry Authors: Aliaga ME, López-Alarcón C, Bridi R, Speisky H Tags: J Inorg Biochem Source Type: research

Insight into structural rearrangements and interdomain interactions related to electron transfer between flavin mononucleotide and heme in nitric oxide synthase: A molecular dynamics study.
Abstract Calmodulin (CaM) binding to nitric oxide synthase (NOS) enables a conformational change, in which the FMN domain shuttles between the FAD and heme domains to deliver electrons to the active site heme center. A clear understanding of this large conformational change is critical, since this step is the rate-limiting in NOS catalysis. Herein molecular dynamics simulations were conducted on a model of an oxygenase/FMN (oxyFMN) construct of human inducible NOS (iNOS). This is to investigate the structural rearrangements and the domain interactions related to the FMN-heme interdomain electron transfer (IET). We...
Source: Journal of Inorganic Biochemistry - August 7, 2015 Category: Biochemistry Authors: Sheng Y, Zhong L, Guo D, Lau G, Feng C Tags: J Inorg Biochem Source Type: research

Functional fluorescent nonporous silica nanoparticles as carriers for Pt(IV) anticancer prodrugs.
Abstract Multilayer fluorescent nonporous silica nanoparticles (SNPs) with an external shell containing primary amino groups were used as delivery systems for Pt(IV) candidate antitumor prodrugs. Spherical SNPs of three different sizes (diameter around 120, 100, and 50nm) were loaded with two different complexes, namely (OC-6-33)-diamminebis(4-carboxybutanoato)dichloridoplatinum(IV) (1) and (OC-6-44)-diammine(4-carboxybutanoato)dichloridoethanolatoplatinum(IV) (2), through the formation of amide bonds between the pendant amino groups on SNPs and the free carboxylic groups of the complexes. Complex 1 proved to caus...
Source: Journal of Inorganic Biochemistry - August 6, 2015 Category: Biochemistry Authors: Ravera M, Perin E, Gabano E, Zanellato I, Panzarasa G, Sparnacci K, Laus M, Osella D Tags: J Inorg Biochem Source Type: research

Nanomolar aluminum induces expression of the inflammatory systemic biomarker C-reactive protein (CRP) in human brain microvessel endothelial cells (hBMECs).
Abstract C-reactive protein (CRP; also known as pentraxin 1, PTX1), a 224 amino acid soluble serum protein organized into a novel pentameric ring-shaped structure, is a highly sensitive pathogenic biomarker for systemic inflammation. High CRP levels are found in practically every known inflammatory state, and elevated CRP levels indicate an increased risk for several common age-related human degenerative disorders, including cardiovascular disease, cancer, diabetes, and Alzheimer's disease (AD). While the majority of CRP is synthesized in the liver for secretion into the systemic circulation, it has recently been ...
Source: Journal of Inorganic Biochemistry - August 1, 2015 Category: Biochemistry Authors: Alexandrov PN, Kruck TP, Lukiw WJ Tags: J Inorg Biochem Source Type: research

Metal coordination and tyrosinase inhibition studies with Kojic-βAla-Kojic.
Metal coordination and tyrosinase inhibition studies with Kojic-βAla-Kojic. J Inorg Biochem. 2015 Jul 26; Authors: Lachowicz JI, Nurchi VM, Crisponi G, Pelaez MG, Rescigno A, Stefanowicz P, Cal M, Szewczuk Z Abstract Kojic acid is a natural antifungal and antibacterial agent that has been extensively studied for its tyrosinase inhibitory and metal coordination properties. Tyrosinase is a metalloenzyme with two copper ions in the active site. It is widely accepted that the tyrosinase inhibitory activity of kojic acid is related to its ability to coordinate metals. Over the past five years, we have...
Source: Journal of Inorganic Biochemistry - July 26, 2015 Category: Biochemistry Authors: Lachowicz JI, Nurchi VM, Crisponi G, Pelaez MG, Rescigno A, Stefanowicz P, Cal M, Szewczuk Z Tags: J Inorg Biochem Source Type: research

The reaction of a platinated methionine motif of CTR1 with cysteine and histidine is dependent upon the type of precursor platinum complex.
Abstract The human copper protein (hCTR1) is believed to facilitate the cellular uptake of cisplatin. Cisplatin likely binds to the methionine (Met)-rich motifs located in the N-terminus of hCTR1, and ligand exchange would be essential if cisplatin has to pass through the hCTR1 channel. In this work, we investigated the reaction between platinated adducts of a methionine-rich motif of yeast CTR1 (Mets7) and N-acetyl-cysteine (AcCys) or N-acetyl-histidine (AcHis), mimicking metal-binding residues downstream the CTR1 channel. Platination involved two cis-compounds, cisplatin and oxaliplatin, and one monofunctional c...
Source: Journal of Inorganic Biochemistry - July 21, 2015 Category: Biochemistry Authors: Ma G, Wu Q, Wu X, Arnesano F, Natile G, Sletten E, Liu Y Tags: J Inorg Biochem Source Type: research

Interactions of carboplatin and oxaliplatin with proteins: Insights from X-ray structures and mass spectrometry studies of their ribonuclease A adducts.
Abstract Oxaliplatin and carboplatin are two platinum(II) drugs in widespread clinical use for the treatment of various types of cancers; yet, structural information on their interactions with proteins is scarce. Here, the X-ray structures of the adducts formed upon reaction of carboplatin and oxaliplatin with bovine pancreatic ribonuclease (RNase A) are reported and compared with results obtained for the structure of the RNase A-cisplatin adduct derived from isomorphous crystals, under the same experimental conditions. Additional details on the binding mode of these metallodrugs toward RNase A are provided by ele...
Source: Journal of Inorganic Biochemistry - July 21, 2015 Category: Biochemistry Authors: Messori L, Marzo T, Merlino A Tags: J Inorg Biochem Source Type: research

Oxidative DNA cleavage by Cu(II) complexes: Effect of periphery substituent groups.
Abstract A series of structurally-related [Cu(R-benzyl-dipicolylamine)(NO3)2] complexes, where R=methoxy- (1), methyl- (2), H- (3), fluoro- (4), and nitro-group (5), were synthesized, and their activity on DNA cleavage was investigated by linear dichroism (LD) and electrophoresis. The addition of a benzyl group to the dipicolylamine ligand of the [Cu(dipicolylamine)(NO3)2] complex (A), i.e., the [Cu(benzyl-dipicolylamine)(NO3)2] complex (3), caused significant enhancement in the efficiency of oxidative cleavage of both super-coiled (sc) and double stranded (ds) DNA, as evidenced by the electrophoresis pattern and ...
Source: Journal of Inorganic Biochemistry - July 20, 2015 Category: Biochemistry Authors: Wang W, Lee YA, Kim G, Kim SK, Lee GY, Kim J, Kim Y, Park GJ, Kim C Tags: J Inorg Biochem Source Type: research

Progressive inflammatory pathology in the retina of aluminum-fed 5xFAD transgenic mice.
Abstract At least 57 murine transgenic models for Alzheimer's disease (Tg-AD) have been developed to overexpress the 42 amino acid amyloid-beta (Aβ42) peptide in the central nervous system (CNS). These 'humanized murine Tg-AD models' have greatly expanded our understanding of the contribution of Aβ42 peptide-mediated pro-inflammatory neuropathology to the AD process. A number of independent laboratories using different amyloid-overexpressing Tg-AD models have shown that supplementation of murine Tg-AD diets and/or drinking water with aluminum significantly enhances Aβ42 peptide-mediated inflammatory...
Source: Journal of Inorganic Biochemistry - July 20, 2015 Category: Biochemistry Authors: Ai P, P D, Jm H, Wj L Tags: J Inorg Biochem Source Type: research

The crystal structure of heme acquisition system A from Yersinia pseudotuberculosis (HasAypt): Roles of the axial ligand Tyr75 and two distal arginines in heme binding.
We report here crystal structures of heme acquisition system A from Yersinia pseudotuberculosis (HasAypt) and its Y75A mutant. The wild-type HasAypt structure revealed that the heme iron is coordinated with Tyr75 and a water molecule. The heme-bound water molecule makes extensive hydrogen bond network that includes Arg40 and Arg144 on the distal heme pocket. Arg40, highly conserved for HasAs from Yersinia species, forms a salt bridge with the propionate side chain of heme, and makes π-π stacking and hydrophobic interactions with porphyrin plane. Interestingly, similar Arg-heme interactions are also found for periplas...
Source: Journal of Inorganic Biochemistry - July 18, 2015 Category: Biochemistry Authors: Kanadani M, Sato T, Hino T, Nagano S, Ozaki SI Tags: J Inorg Biochem Source Type: research

Preparation, stability, and photoreactivity of thiolato ruthenium polypyridyl complexes: Can cysteine derivatives protect ruthenium-based anticancer complexes?
t S Abstract Ruthenium polypyridyl complexes may act as light-activatable anticancer prodrugs provided that they are protected by well-coordinated ligands that i) prevent coordination of other biomolecules to the metal center in the dark and ii) can be removed by visible light irradiation. In this paper, the use of monodentate thiol ligands RSH as light-cleavable protecting groups for the ruthenium complex [Ru(tpy)(bpy)(OH2)](PF6)2 ([1](PF6)2; tpy=2,2';6',2″-terpyridine, bpy=2,2'-bypyridine), is investigated. The reaction of [1](2+) with RSH=H2Cys (l-cysteine), H2Acys (N-acetyl-l-cysteine), and HAcysMe (N-ac...
Source: Journal of Inorganic Biochemistry - July 10, 2015 Category: Biochemistry Authors: van Rixel VH, Busemann A, Göttle AJ, Bonnet S Tags: J Inorg Biochem Source Type: research

Copper(II) complexes of terminally free alloferon peptide mutants containing two different histidyl (H(1) and H(6) or H(9) or H(12)) binding sites Structure Stability and Biological Activity.
Abstract Mono- and dinuclear copper(II) complexes of the alloferon 1 with point mutations H9A/H12A H(1)GVSGH(6)GQA(9)GVA(12)G, H6A/H12A H(1)GVSGA(6)GQH(9)GVA(12)G and H6A/H9A H(1)GVSGA(6)GQA(9)GVH(12)G have been studied by potentiometric, UV-visible, CD, EPR spectroscopic, and mass spectrometry (MS) methods. Complete complex speciation at metal-to-ligand molar ratios 1:1 and 2:1 was obtained. For all systems studied in the 5 - 6.5 pH range, the CuL complex dominates with 3N{NH2,NIm-H(1),NIm-H(6 or 9 or 12)} binding site. The stability of the CuL complexes for the ligands studied varies according to the H9A/H12A>...
Source: Journal of Inorganic Biochemistry - July 3, 2015 Category: Biochemistry Authors: Matusiak A, Kuczer M, Czarniewska E, Urbański A, Rosiński G, Kowalik-Jankowska T Tags: J Inorg Biochem Source Type: research

Preface for the JIB Special Issue for the 12th European Biological Inorganic Chemistry Conference (EuroBIC 12) in Zurich, Switzerland, August 24-28, 2014.
PMID: 26100006 [PubMed - in process] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - June 27, 2015 Category: Biochemistry Authors: Freisinger E, Sigel R Tags: J Inorg Biochem Source Type: research