Preface.
te;s-Gutiérrez J PMID: 23993869 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - August 14, 2013 Category: Biochemistry Authors: Domínguez-Martín A, Niclós-Gutiérrez J Tags: J Inorg Biochem Source Type: research

Condensation and salt-induced decondensation of DNA upon incorporation of a V-shaped luminescent [Ru2(bpy)4(mbpibH2)](4+)
Abstract This paper first reports on the condensation of DNA to a tightly packed state induced by a V-shaped di-ruthenium(II) complex [Ru2(bpy)4(mbpibH2)]Cl4 (bpy=2,2'-bipyridine and mbpibH2=1,3-bis([1,10]phenanthroline[5,6-d]imidazol-2-yl)benzene), which binds to the groove of herring sperm DNA (hsDNA) with the binding constant of 2.0×10(7)M(-1) (0.05M NaCl, pH7.2). The di-Ru(II) complex is found to induce the condensation of both hsDNA to long chain-like particle clusters and originally circular plasmid pBR322 DNA to particulate structure under neutral conditions. More interestingly, the presence of NaCl h...
Source: Journal of Inorganic Biochemistry - August 14, 2013 Category: Biochemistry Authors: Gan GL, Chao H, Cai XP, Jiang ZS, Li H Tags: J Inorg Biochem Source Type: research

A dinuclear zinc(II) complex of a new unsymmetric ligand with an N5O2 donor set; A structural and functional model for the active site of zinc phosphoesterases.
Abstract The dinuclear complex [Zn2(DPCPMP)(pivalate)](ClO4), where DPCPMP is the new unsymmetrical ligand [2-(N-(3-((bis((pyridin-2-yl)methyl)amino)methyl)-2-hydroxy-5-methylbenzyl)-N-((pyridin-2-yl)methyl)amino)acetic acid], has been synthesized and characterized. The complex is a functional model for zinc phosphoesterases with dinuclear active sites. The hydrolytic efficacy of the complex has been investigated using bis-(2,4-dinitrophenyl)phosphate (BDNPP), a DNA analog, as substrate. Speciation studies using potentiometric titrations have been performed for both the ligand and the corresponding dizinc complex ...
Source: Journal of Inorganic Biochemistry - August 14, 2013 Category: Biochemistry Authors: Das B, Daver H, Pyrkosz-Bulska M, Persch E, Barman SK, Mukherjee R, Gumienna-Kontecka E, Jarenmark M, Himo F, Nordlander E Tags: J Inorg Biochem Source Type: research

Gold(III) complexes with esters of cyclohexyl-functionalized ethylenediamine-N,N'-diacetate.
Abstract Six novel gold(III) complexes containing O,O'-dialkyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate ([AuCl2{(S,S)-R2eddch}]PF6, R=Me, Et, n-Pr, n-Bu, i-Bu, i-Am; 1-6, respectively) were synthesized and characterized by elemental analysis, UV/Visible, IR and NMR spectroscopy, mass spectrometry and differential pulse voltammetry. Density functional theory (DFT) calculations confirmed that diastereoisomer with the N,N' atoms configured (S,S) was the most stable. In vitro antiproliferative activity was determined against human cervix adenocarcinoma HeLa and human myelogenous leukemia K562 tumor cel...
Source: Journal of Inorganic Biochemistry - August 13, 2013 Category: Biochemistry Authors: Pantelić N, Zmejkovski BB, Trifunović-Macedoljan J, Savić A, Stanković D, Damjanović A, Juranić Z, Kaluđerović GN, Sabo TJ Tags: J Inorg Biochem Source Type: research

Effects of chrysin, apigenin, genistein and their homoleptic copper(II) complexes on the growth and metastatic potential of cancer cells.
Abstract The (iso-)flavonoids chrysin 1, apigenin 2, genistein 3 and their homoleptic copper(II) complexes 4-6 were compared for general cancer cell growth inhibition and for antimetastatic effects on rapidly proliferating and metastasizing 518A2 melanoma cells. The complexes 4-6 were three to five times more active than the free flavonoids in cytotoxicity assays with MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] against 518A2 melanoma, HCT-116 colon, KB-V1/Vbl cervix, and MCF-7/Topo breast carcinoma cells. This activity correlated with an arrest of the cell cycle of 518A2 melanoma cells at th...
Source: Journal of Inorganic Biochemistry - August 3, 2013 Category: Biochemistry Authors: Spoerlein C, Mahal K, Schmidt H, Schobert R Tags: J Inorg Biochem Source Type: research

Sequence-specific Ni(II)-dependent peptide bond hydrolysis for protein engineering: Active sequence optimization.
Abstract In previous studies we showed that Ni(II) ions can hydrolytically cleave a peptide bond preceding Ser/Thr in peptides of a general sequence RN-(Ser/Thr)-Xaa-His-Zaa-RC, where RN and RC are any peptide sequences. A peptide library screening, assisted by accurate measurements of reaction kinetics for selected peptides, demonstrated the preference for bulky and aromatic residues at variable positions Xaa and Zaa [A. Krężel, E. Kopera, A.M. Protas, A. Wysłouch-Cieszyńska, J. Poznański, W. Bal, J. Am. Chem. Soc., 132 (2010) 3355-3366]. In this work we used a similar strategy to find out whether the next r...
Source: Journal of Inorganic Biochemistry - August 2, 2013 Category: Biochemistry Authors: Protas AM, Ariani HH, Bonna A, Polkowska-Nowakowska A, Poznański J, Bal W Tags: J Inorg Biochem Source Type: research

Determination of formal redox potentials in aqueous solution of copper(II) complexes with ligands having nitrogen and oxygen donor atoms and comparison with their EPR and UV-Vis spectral features.
mo RP Abstract Formal redox potentials in aqueous solution were determined for copper(II) complexes with ligands having oxygen and nitrogen as donor atoms. All the chosen copper(II) complexes have well-known stereochemistries (pseudo-octahedral, square planar, square-based pyramidal, trigonal bipyramidal or tetrahedral) as witnessed by their reported spectroscopic, EPR and UV-visible (UV-Vis) features, so that a rough correlation between the measured redox potential and the typical geometrical arrangement of the copper(II) complex could be established. Negative values have been obtained for copper(II) complexes in...
Source: Journal of Inorganic Biochemistry - August 2, 2013 Category: Biochemistry Authors: Tabbì G, Giuffrida A, Bonomo RP Tags: J Inorg Biochem Source Type: research

Synthesis, structure and urease inhibition studies of Schiff base copper(II) complexes with planar four-coordinate copper(II) centers.
Abstract Seven new Schiff base copper(II) complexes with planar four-coordinate copper(II) centers have been synthesized and structurally characterized. The solid state structures of complexes 1, 3, 4, 5, 6 and 7 present a square-planar coordination geometry at the metal centers while complex 2 shows a slightly distorted square-planar geometry. Density functional theory calculations have been performed to evaluate the electronic structure of copper(II) complex 7. Inhibition of jack bean urease by copper(II) complexes 1-7 have also been investigated, and potent inhibitory activities with IC50 range of 2.60-17.00&mu...
Source: Journal of Inorganic Biochemistry - August 2, 2013 Category: Biochemistry Authors: Dong X, Guo T, Li Y, Cui Y, Wang Q Tags: J Inorg Biochem Source Type: research

Intercalative interaction of asymmetric copper(II) complex with DNA: Experimental, molecular docking, molecular dynamics and TDDFT studies.
Abstract The intercalative interactions of small molecules with DNA are important in a variety of biological processes including mutagenesis, carcinogenesis, and chemotherapy. A comprehensive research protocol including experiments and calculations was employed to investigate the intercalative interaction between metallointercalator copper(II) complex and DNA. The intercalative binding mode has been validated by UV spectra, fluorescence spectra, CD spectra and viscosity measurements. The classical molecular dynamics simulation was carried out to investigate the intercalative interaction between asymmetric copper(I...
Source: Journal of Inorganic Biochemistry - July 31, 2013 Category: Biochemistry Authors: Hu W, Deng S, Huang J, Lu Y, Le X, Zheng W Tags: J Inorg Biochem Source Type: research

Organometallic ruthenium anticancer complexes inhibit human glutathione-S-transferase π
Organometallic ruthenium anticancer complexes inhibit human glutathione-S-transferase π J Inorg Biochem. 2013 Jul 25;128C:77-84 Authors: Lin Y, Huang Y, Zheng W, Wang F, Habtemariam A, Luo Q, Li X, Wu K, Sadler PJ, Xiong S Abstract The organometallic ruthenium(II) anticancer complexes [(η(6)-arene)Ru(en)Cl](+) (arene=p-cymene (1), biphenyl (2) or 9,10-dihydrophenanthrene (3); en=ethylenediamine), exhibit in vitro and in vivo anticancer activities. In the present work, we show that they inhibit human glutathione-S-transferase π (GSTπ) with IC50 values of 59.4±1.3, 63.2±0.4 and 37....
Source: Journal of Inorganic Biochemistry - July 25, 2013 Category: Biochemistry Authors: Lin Y, Huang Y, Zheng W, Wang F, Habtemariam A, Luo Q, Li X, Wu K, Sadler PJ, Xiong S Tags: J Inorg Biochem Source Type: research

Accumulation, elimination, and effects of parenteral exposure to aluminum in newborn and adult rats.
This study evaluated the effect of the administration of an equivalent amount of Al (0.12mg/kg/day) to newborn rats. The study included the administration of a higher amount of Al (24.8mg/kg/day) not only to newborn rats but also to adult (2- and 4-month-old) rats. Aluminum was intraperitoneally administered for a period of 10days. Newborn animals were evaluated for developmental changes every day starting from the second day after birth. Twenty days after the last administration, 10 animals were killed and their organs were removed; the remainders were killed on day 40. A dosage of 24.8mg/kg/day was administered to the tw...
Source: Journal of Inorganic Biochemistry - July 22, 2013 Category: Biochemistry Authors: Veiga M, Bohrer D, Banderó CR, Oliveira SM, do Nascimento PC, Mattiazzi P, Mello CF, Lenz QF, Oliveira MS Tags: J Inorg Biochem Source Type: research

Distinctive clinical features in arthro-myalgic patients with and without aluminum hydroxyde-induced macrophagic myofasciitis: An exploratory study.
Abstract Macrophagic myofasciitis (MMF) is a specific histological lesion assessing the persistence of vaccine-derived aluminum oxyhydroxide in muscle tissue, at a site of previous immunization. Long-lasting MMF is usually detected in patients with arthromyalgias, chronic fatigue, and stereotyped cognitive dysfunction. MMF diagnosis requires muscle biopsy, an invasive procedure not suitable for the routine investigation of all patients with musculoskeletal pain. To help decision making in routine practice, we designed a retrospective analysis of 130 consecutive arthro-myalgic patients, previously immunized with al...
Source: Journal of Inorganic Biochemistry - July 20, 2013 Category: Biochemistry Authors: Ragunathan-Thangarajah N, Le Beller C, Boutouyrie P, Bassez G, Gherardi RK, Laurent S, Authier FJ Tags: J Inorg Biochem Source Type: research

Structure and function of heme proteins in non-native states: A mini-review.
Abstract Heme proteins perform various biological functions ranging from electron transfer, oxygen binding and transport, catalysis, to signaling. Although adopting proper native states is very important for these functions, progresses in representative heme proteins, including cytochrome c (cyt c), cytochrome b5 (cyt b5), myoglobin (Mb), neuroglobin (Ngb), cytochrome P450 (CYP) and heme-based sensor proteins such as CO sensor CooA, showed that various native functions, or new functions evolved, are also closely associated with non-native states. The structure and function relationship of heme proteins in non-nati...
Source: Journal of Inorganic Biochemistry - July 19, 2013 Category: Biochemistry Authors: Lin YW, Wang J Tags: J Inorg Biochem Source Type: research

Synthesis, characterization and cell viability test of six vanadyl complexes with acetylacetonate derivatives.
Abstract Vanadium compounds are known to display a number of therapeutic effects, namely insulin-mimetic and cardiovascular effects. Evidence of the antiproliferative and proapoptotic activity of a number of vanadyl complexes, together with their low toxicity, establishes these metal compounds as promising antitumoral therapeutic agents. In the present work, we describe the synthesis and full characterization of six new vanadyl complexes with acetylacetonate derivatives bearing asymmetric substitutions on the β-dicarbonyl moiety: the complexes were characterized in the solid state as well as in solution. Our ...
Source: Journal of Inorganic Biochemistry - July 18, 2013 Category: Biochemistry Authors: Sgarbossa S, Diana E, Marabello D, Deagostino A, Cadamuro S, Barge A, Laurenti E, Gallicchio M, Boscaro V, Ghibaudi E Tags: J Inorg Biochem Source Type: research

Isomorphic deactivation of a Pseudomonas aeruginosa oxidoreductase: The crystal structure of Ag(I) metallated azurin at 1.7Å
Isomorphic deactivation of a Pseudomonas aeruginosa oxidoreductase: The crystal structure of Ag(I) metallated azurin at 1.7Å J Inorg Biochem. 2013 Jul 16;128C:11-16 Authors: Panzner MJ, Bilinovich SM, Parker JA, Bladholm EL, Ziegler CJ, Berry SM, Leeper TC Abstract Multiple biophysical methods demonstrate that silver effectively metallates Pseudomonas aeruginosa apo-azurin in solution. X-ray crystallography of the silver-modified protein reveals that silver binds to azurin at the traditional copper mediated active site with nearly identical geometry. Cyclic voltammetry indicates that the silver adduc...
Source: Journal of Inorganic Biochemistry - July 16, 2013 Category: Biochemistry Authors: Panzner MJ, Bilinovich SM, Parker JA, Bladholm EL, Ziegler CJ, Berry SM, Leeper TC Tags: J Inorg Biochem Source Type: research

Binary and ternary mixed metal complexes of terminally free peptides containing two different histidyl binding sites.
na D, Sóvágó I Abstract Copper(II), nickel(II) and zinc(II) complexes of the terminally free peptides AHAAAHG and AAHAAAHG have been studied by combined applications of potentiometric and various spectroscopic techniques, including UV-visible, CD and EPR for copper(II) and UV-visible, CD and NMR for nickel(II). It was found that the octapeptide AAHAAAHG can easily bind two equivalents of copper(II) or nickel(II) ions and the amino terminus was identified as the primary ligating site of the molecule. On the other hand, this peptide has a relatively low zinc(II) binding affinity. Mono- and di-nu...
Source: Journal of Inorganic Biochemistry - July 16, 2013 Category: Biochemistry Authors: Grenács A, Kaluha A, Kállay C, Jószai V, Sanna D, Sóvágó I Tags: J Inorg Biochem Source Type: research

Inhibition of human prion neuropeptide PrP106-126 aggregation by hexacoordinated ruthenium complexes.
This study provided better understanding on the interaction of metal complexes with PrP106-126 and paved the way for potential Ru-based metallodrugs against prion diseases. PMID: 23911565 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - July 15, 2013 Category: Biochemistry Authors: Wang X, Zhang B, Zhao C, Wang Y, He L, Cui M, Zhu X, Du W Tags: J Inorg Biochem Source Type: research

Amelioration of iron toxicity: A mechanism for aluminum-induced growth stimulation in tea plants.
In this study, hydroponically grown tea plants treated with 0 to 300μM Al were analyzed for growth, Al and Fe accumulation, and Al distribution by means of morin and hematoxylin staining. Roots of control plants stained black with hematoxylin. This indicates the formation of a Fe-hematoxylin complex. Young leaves of controls accumulated more than 1000mgFekg(-1) dry weight. This concentration is above the Fe-toxicity threshold in most species. Supply of Al stimulated growth and reduced Fe uptake and transport. These results indicate that Al-induced growth stimulation might be due to alleviation of a latent Fe toxicity oc...
Source: Journal of Inorganic Biochemistry - July 13, 2013 Category: Biochemistry Authors: Hajiboland R, Barceló J, Poschenrieder C, Tolrà R Tags: J Inorg Biochem Source Type: research

Aluminium and breast cancer: Sources of exposure, tissue measurements and mechanisms of toxicological actions on breast biology.
Abstract This review examines recent evidence linking exposure to aluminium with the aetiology of breast cancer. The human population is exposed to aluminium throughout daily life including through diet, application of antiperspirants, use of antacids and vaccination. Aluminium has now been measured in a range of human breast structures at higher levels than in blood serum and experimental evidence suggests that the tissue concentrations measured have the potential to adversely influence breast epithelial cells including generation of genomic instability, induction of anchorage-independent proliferation and interf...
Source: Journal of Inorganic Biochemistry - July 13, 2013 Category: Biochemistry Authors: Darbre PD, Mannello F, Exley C Tags: J Inorg Biochem Source Type: research

First polymer "ruthenium-cyclopentadienyl" complex as potential anticancer agent.
First polymer "ruthenium-cyclopentadienyl" complex as potential anticancer agent. J Inorg Biochem. 2013 Jul 12;127C:79-81 Authors: Valente A, Garcia MH, Marques F, Miao Y, Rousseau C, Zinck P Abstract d-glucose end-capped polylactide ruthenium cyclopentadienyl complex (RuPMC) was newly synthesized by a straightforward method. RuPMC was tested against human MCF7 and MDAMB231 breast and A2780 ovarian adenocarcinoma revealing IC50 values in the micromolar range. A pH dependent hydrolysis is advanced by preliminary UV-visible spectroscopy. Cellular distribution studies showed that RuPMC is predominan...
Source: Journal of Inorganic Biochemistry - July 12, 2013 Category: Biochemistry Authors: Valente A, Garcia MH, Marques F, Miao Y, Rousseau C, Zinck P Tags: J Inorg Biochem Source Type: research

Effect of aluminium on migratory and invasive properties of MCF-7 human breast cancer cells in culture.
Abstract Aluminium (Al) has been measured in human breast tissue, nipple aspirate fluid and breast cyst fluid, and recent studies have shown that at tissue concentrations, aluminium can induce DNA damage and suspension growth in human breast epithelial cells. This paper demonstrates for the first time that exposure to aluminium can also increase migratory and invasive properties of MCF-7 human breast cancer cells. Long-term (32weeks) but not short-term (1week) exposure of MCF-7 cells to 10(-4)M aluminium chloride or 10(-4)M aluminium chlorohydrate increased motility of the cells as measured by live cell imaging (c...
Source: Journal of Inorganic Biochemistry - July 12, 2013 Category: Biochemistry Authors: Darbre PD, Bakir A, Iskakova E Tags: J Inorg Biochem Source Type: research

A novel mechanism of aluminium-induced cell death involving vacuolar processing enzyme and vacuolar collapse in tobacco cell line BY-2.
Abstract The role of vacuole in the cell death mechanism induced by aluminium (Al) was investigated in tobacco (Nicotiana tabacum L.) cell line BY-2. Cells at logarithmic phase of growth were treated without (control) or with Al (up to 150μM) in a treatment medium containing CaCl2, sucrose and 2-(N-morpholino) ethanesulfonic acid (MES) buffer (pH5.0). After 18h treatment, both the integrity of the plasma membrane (estimated by Evans blue uptake) and growth capacity (estimated by post-Al treatment growth in nutrient medium) were decreased, while the activity of vacuolar processing enzyme (VPE) was increased, in ...
Source: Journal of Inorganic Biochemistry - July 8, 2013 Category: Biochemistry Authors: Kariya K, Demiral T, Sasaki T, Tsuchiya Y, Turkan I, Sano T, Hasezawa S, Yamamoto Y Tags: J Inorg Biochem Source Type: research

From coordination chemistry to biological chemistry of aluminium.
Abstract The paper gives a review on the importance of distribution of Al in biological fluids, primarily in the lights of the works of the author in Al chemistry. It starts with studies of interactions of Al(III) with small biomolecules, such as aliphatic and aromatic hydroxycarboxylic acids, and inorganic and organic phosphates. A significant part of this review deals with the problems of description of the biospeciation of Al(III) in serum, where besides the thermodynamic conditions the role of time is also considered in the case of this sluggish metal ion. The Al(III) complexes of the other large group of biom...
Source: Journal of Inorganic Biochemistry - July 4, 2013 Category: Biochemistry Authors: Kiss T Tags: J Inorg Biochem Source Type: research

A rice fungicide blasticidin S efficiently binds Cu(II) ions and prevents DNA from metal-induced damage.
Abstract Blasticidin S is a representative of the aminoacylnucleoside class of antibiotics and it possesses fungicidal properties against the virulent fungus which causes a serious rice blast disease in Asia. It is widely used to control rice blast by foliar application as a crop protection agent. Serious copper contamination is observed in some areas of China. Moreover, some paddy soils present a potential risk of copper accumulation in the human body through the food chain, leading to several disorders. This work presents the results of combined potentiometric and spectroscopic (UV-visible, EPR, CD, NMR) data wh...
Source: Journal of Inorganic Biochemistry - June 29, 2013 Category: Biochemistry Authors: Stokowa-Sołtys K, Jeżowska-Bojczuk M Tags: J Inorg Biochem Source Type: research

Phthalocyanines functionalized with 2-methyl-5-nitro-1H-imidazolylethoxy and 1,4,7-trioxanonyl moieties and the effect of metronidazole substitution on photocytotoxicity.
inski T Abstract Four novel magnesium(II) and zinc(II) phthalocyanines bearing 1,4,7-trioxanonyl, polyether and/or (2-methyl-5-nitro-1H-imidazol-1-yl)ethoxy, heterocyclic substituents at their non-peripheral positions were synthesized and assessed in terms of physicochemical and biological properties. Magnesium phthalocyanine derivatives bearing polyether substituents (Pc-1), a mixed system of polyether and heterocyclic substituents (Pc-3), and four heterocyclic substituents (Pc-4), respectively, were synthesized following the Linstead macrocyclization reaction procedure. Zinc phthalocyanine (Pc-2) bearing polyeth...
Source: Journal of Inorganic Biochemistry - June 28, 2013 Category: Biochemistry Authors: Wierzchowski M, Sobotta L, Skupin-Mrugalska P, Kruk J, Jusiak W, Yee M, Konopka K, Düzgüneş N, Tykarska E, Gdaniec M, Mielcarek J, Goslinski T Tags: J Inorg Biochem Source Type: research

A family of hydroxypyrone ligands designed and synthesized as iron chelators.
, Choquesillo-Lazarte D, Szewczuk Z Abstract The use of chelating agents for iron and aluminum in different clinical applications has found increasing attention in the last thirty years. Desferal, deferiprone and deferasirox, chelating agents nowadays in use, are based on hydroxamic groups, hydroxyl-substituted pyridinones or aromatic ring systems. With the aim of designing new chelators, we synthesized a series of kojic acid derivatives composed by two kojic units joined by linkers variously substituted. The huge advantages of these molecules are that they are easy and cheap to produce. Preliminary works on compl...
Source: Journal of Inorganic Biochemistry - June 26, 2013 Category: Biochemistry Authors: Toso L, Crisponi G, Nurchi VM, Crespo-Alonso M, Lachowicz JI, Santos MA, Marques SM, Niclós-Gutiérrez J, González-Pérez JM, Domínguez-Martín A, Choquesillo-Lazarte D, Szewczuk Z Tags: J Inorg Biochem Source Type: research

Alteration of aluminium inhibition of synaptosomal (Na(+)/K(+))ATPase by colestipol administration.
es PP Abstract The ability of aluminium to inhibit the (Na(+)/K(+))ATPase activity has been observed by several authors. During chronic dietary exposure to AlCl3, brain (Na(+)/K(+))ATPase activity drops, even if no alterations of catalytic subunit protein expression and of energy charge potential are observed. The aluminium effect on (Na(+)/K(+))ATPase activity seems to implicate the reduction of interacting protomers within the oligomeric ensemble of the membrane-bound (Na(+)/K(+))ATPase. The activity of (Na(+)/K(+))ATPase is altered by the microviscosity of lipid environment. We studied if aluminium inhibitory e...
Source: Journal of Inorganic Biochemistry - June 24, 2013 Category: Biochemistry Authors: Silva VS, Oliveira L, Gonçalves PP Tags: J Inorg Biochem Source Type: research

Synthesis and characterization of ruthenium(II)-oligopyridine-peptide conjugates. Interactions of the diasteromeres Δ- and Λ-[Ru(bpy)2(4-COY-4'-Mebpy)]Cl2 (Y=Gly-Lys(1)-Lys(2)CONH2, Lys(1)-Gly-Lys(2)CONH2, Lys(1)-Lys(2)-GlyCONH2) with the oligonucleotide d(5'-CGCGAATTCGCG-3')2.
Synthesis and characterization of ruthenium(II)-oligopyridine-peptide conjugates. Interactions of the diasteromeres Δ- and Λ-[Ru(bpy)2(4-COY-4'-Mebpy)]Cl2 (Y=Gly-Lys(1)-Lys(2)CONH2, Lys(1)-Gly-Lys(2)CONH2, Lys(1)-Lys(2)-GlyCONH2) with the oligonucleotide d(5'-CGCGAATTCGCG-3')2. J Inorg Biochem. 2013 Jun 24;127C:13-23 Authors: Karidi K, Ypsilantis K, Papakyriakou A, Garoufis A Abstract Diastereomeric complexes of the general formulae Λ- and Δ-[Ru(bpy)2(4-COY-4'-Mebpy)]Cl2 where bpy=2,2'-bipyridine and Y=Gly-Lys(1)-Lys(2)CONH2, Lys(1)-Gly-Lys(2)CONH2, Lys(1)-Lys(2)-GlyCONH2, were sy...
Source: Journal of Inorganic Biochemistry - June 24, 2013 Category: Biochemistry Authors: Karidi K, Ypsilantis K, Papakyriakou A, Garoufis A Tags: J Inorg Biochem Source Type: research

Molecular recognition modes between adenine or adeniniun(1+) ion and binary M(II)(pdc) chelates (MCoZn; pdc=pyridine-2,6-dicarboxylate(2-) ion).
tilde;eiras A, Niclós-Gutiérrez J Abstract Mixed ligand M(II)-complexes (MCoZn) with pyridine-2,6-dicarboxylate(2-) chelator (pdc) and adenine (Hade) have been synthesized and studied by X-ray diffraction and other spectral and thermal methods: [Cu(pdc)(H(N9)ade)(H2O)] (1), [Cu2(pdc)2(H2O)2(μ2-N3,N7-H(N9)ade)]·3H2O (2), trans-[M(pdc)(H(N9)ade)(H2O)2]·nH2O for MCo (3-L, 3-M, 3-H) or Zn (4-L, 4-H), where n is 0, 1 or 3 for the 'lowest' (L), 'medium' (M) and 'highest' (H) hydrated forms, and the salt trans-[Ni(pdc)(H2(N1,N9)ade)(H2O)2]Cl·2H2O (5). In all the nine compounds, both ...
Source: Journal of Inorganic Biochemistry - June 21, 2013 Category: Biochemistry Authors: Del Pilar Brandi-Blanco M, Choquesillo-Lazarte D, Domínguez-Martín A, Matilla-Hernández A, González-Pérez JM, Castiñeiras A, Niclós-Gutiérrez J Tags: J Inorg Biochem Source Type: research

Antiproliferative effects of palladium(II) complexes of 5-nitrosopyrimidines and interactions with the proteolytic regulatory enzymes of the renin-angiotensin system in tumoral brain cells.
oacute;sito MJ, Moreno-Carretero MN Abstract Seventeen new palladium(II) complexes of general formulaes PdCl2L, PdCl(LH-1)(solvent) and PdCl2(PPh3)2L containing pyrimidine ligands derived from 6-amino-5-nitrosouracil and violuric acid have been prepared and characterized by elemental analysis, IR and NMR ((1)H and (13)C) methods and, two of them, PdCl(DANUH-1)(CH3CN)]·½H2O and [PdCl(2MeOANUH-1)(CH3CN)] by X-ray single-crystal diffraction (DANU: 6-amino-1,3-dimethyl-5-nitrosouracil; 2MeOANU: 6-amino-2-methoxy-5-nitroso-3H-pyrimidin-4-one). The coordination environment around palladium is nearly square...
Source: Journal of Inorganic Biochemistry - June 18, 2013 Category: Biochemistry Authors: Illán-Cabeza NA, García-García AR, Martínez-Martos JM, Ramírez-Expósito MJ, Moreno-Carretero MN Tags: J Inorg Biochem Source Type: research

The major function of a metallothionein from the aquatic fungus Heliscus lugdunensis is cadmium detoxification.
Abstract A spring from a former copper shale mine in the area of Mansfelder Land, Germany, shows extremely high transition metal ion concentrations, i.e. 40mM Zn(II), 208μM Cu(II), 61μM As(V), and 25μM Cd(II). This makes it a challenging habitat for living organisms as they have to cope with metal ion concentrations that by far exceed the values usually observed in spring water. One of the surviving species found is the aquatic fungus Heliscus lugdunensis (teleomorph: Nectria lugdunensis). Investigation of its redox related heavy metal tolerance revealed the presence of small thiol containing compounds as...
Source: Journal of Inorganic Biochemistry - June 7, 2013 Category: Biochemistry Authors: Loebus J, Leitenmaier B, Meissner D, Braha B, Krauss GJ, Dobritzsch D, Freisinger E Tags: J Inorg Biochem Source Type: research

Competition of zinc(II) with cadmium(II) or mercury(II) in binding to a 12-mer peptide.
, Berkecz R Abstract Speciation of the complexes of zinc(II) with a dodecapeptide (Ac-SCPGDQGSDCSI-NH2), inspired by the metal binding domain of MerR metalloregulatory proteins, have been studied by pH-potentiometric titrations, UV, SRCD (synchrotron radiation circular dichroism) and (1)H NMR experiments. (MerR is a family of transcriptional regulators the archetype of which is the Hg(2+)-responsive transcriptional repressor-activator MerR protein.) The aim of the ligand-design was to retain the advantageous metal binding features of MerR proteins in a model peptide for the efficient capture of toxic metal ions. T...
Source: Journal of Inorganic Biochemistry - June 5, 2013 Category: Biochemistry Authors: Jancsó A, Gyurcsik B, Mesterházy E, Berkecz R Tags: J Inorg Biochem Source Type: research

Insertion of beta-alanine in model peptides for copper binding to His96 and His111 of the human prion protein.
This study clearly shows that two main factors determine the nature of Cu(II)-peptide complexes involving an anchoring His residue and deprotonated amides from the backbone chain: i) the stabilization of Cu(II)-peptide complexes due to the formation of cyclic structures (i.e. chelate effect) and ii) the nature of the residues associated to the deprotonated amide groups that participate in metal ion coordination. PMID: 23796442 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - June 5, 2013 Category: Biochemistry Authors: Rivillas-Acevedo L, Maciel-Barón L, García JE, Juaristi E, Quintanar L Tags: J Inorg Biochem Source Type: research

Insights into the distal heme pocket of H-NOX using fluoride as a probe for H-bonding interactions.
Abstract Nitric oxide (NO) and dioxygen (O2) are gases of similar size, shape, and electrostatic potential, but different physiological function. In aerobic organisms, the cellular concentration of O2 far exceeds that of NO; instead NO relies heavily on the ability of its receptor to discriminate against O2. In mammals, soluble guanylate cyclase (sGC) serves this role, binding NO with picomolar sensitivity and excluding O2 binding. Interestingly, some bacterial homologs of sGC, including the H-NOX (heme-nitric oxide/oxygen) domain from Thermoanaerobacter tengcongensis, tightly bind O2. Three distal pocket residues...
Source: Journal of Inorganic Biochemistry - June 3, 2013 Category: Biochemistry Authors: Kosowicz JG, Boon EM Tags: J Inorg Biochem Source Type: research

Organic cadmium complexes as proteasome inhibitors and apoptosis inducers in human breast cancer cells.
Abstract Although cadmium (Cd) is a widespread environmental contaminant and human carcinogen, our studies indicate an organic Cd complex to be a potent inhibitor of proteasomal chymotrypsin-like (CT-like) activity, further capable of inducing apoptosis in a cancer cell-specific manner. It has been reported that the ligands indole-3-butyric acid (L1) and indole-3-propionic acid (L2) have cancer-fighting effects when tested in a rat carcinoma model. In addition, 3, 5-diaminobenzoic acid o-vanillin Schiff bases (L3) have high antimicrobial activity and a large number of Schiff base complexes have been reported to ha...
Source: Journal of Inorganic Biochemistry - May 23, 2013 Category: Biochemistry Authors: Zhang Z, Bi C, Buac D, Fan Y, Zhang X, Zuo J, Zhang P, Zhang N, Dong L, Dou QP Tags: J Inorg Biochem Source Type: research

Luminescent material based on the [Eu(TTA)3(H2O)2] complex incorporated into modified silica particles for biological applications.
to HF Abstract Amino-functionalized luminescent silica particles were investigated for use in immunoassays. The particles were prepared by the Stöber method where the [Eu(TTA)3(H2O)2] complex (TTA: 3-thenoyltrifluoroacetonate) was incorporated into silica particles during the hydrolysis and condensation of TEOS: tetraethylorthosilicate. Then, the amino groups were introduced in the particle surface using APTS: 3-aminopropyltriethoxisilane. The resulting particles were characterized by scanning electron microscopy (SEM), X ray diffraction (XRD) and photoluminescence spectroscopy. In order to demonstrate the vi...
Source: Journal of Inorganic Biochemistry - May 23, 2013 Category: Biochemistry Authors: Lourenço AV, Kodaira CA, Ramos-Sanchez EM, Felinto MC, Goto H, Gidlund M, Malta OL, Brito HF Tags: J Inorg Biochem Source Type: research

Selective damage to hyphal form through light-induced delivery of nitric oxide to Candida albicans colonies.
Abstract Candida albicans, an opportunistic fungal pathogen, causes severe to life-threatening infections in immunocompromised hosts (e.g. HIV patients, burn victims). Conversion of the commensal yeast form to the invasive hyphal form, triggered by environmental cues, initiates such episodes. Although the antifungal activity of nitric oxide (NO) has been established, very few convenient NO-donating systems for treating C. albicans infection have been reported. In this work, a biocompatible NO-donating material that delivers NO upon illumination with visible light has been employed to eradicate C. albicans in a dos...
Source: Journal of Inorganic Biochemistry - May 23, 2013 Category: Biochemistry Authors: Heilman BJ, Tadle AC, Pimentel LR, Mascharak PK Tags: J Inorg Biochem Source Type: research

Improvement of the antihypertensive capacity of candesartan and trityl candesartan by their SOD mimetic copper(II) complexes.
This study provides a new insight into the development of copper(II) complexes as potential drugs. PMID: 23501135 [PubMed - in process] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - May 23, 2013 Category: Biochemistry Authors: Islas MS, Rojo T, Lezama L, Merino MG, Cortes MA, Puyol MR, Ferrer EG, Williams PA Tags: J Inorg Biochem Source Type: research

Mono- and dicationic Re(I)/(99m)Tc(I) tricarbonyl complexes for the targeting of energized mitochondria.
Abstract The enhanced negative mitochondrial membrane potential of tumor cells can increase the cell accumulation of triphenylphosphonium (TPP) derivatives, which prompted us to investigate TPP-containing Re(I)/(99m)Tc organometallic compounds as probes for in vivo targeting of energized mitochondria. Novel compounds (Re1-Re4/Tc1-Tc4) were obtained with bifunctional chelators of the pyrazole-diamine (N,N,N-donors) and pyrazole-aminocarboxylic (N,N,O-donors) type, functionalized with TPP pharmacophores that have been introduced at the central amine of the chelators using different spacers. In this way, dicationic (...
Source: Journal of Inorganic Biochemistry - May 23, 2013 Category: Biochemistry Authors: Moura C, Mendes F, Gano L, Santos I, Paulo A Tags: J Inorg Biochem Source Type: research

Bio-inspired amino acid oxidation by a non-heme iron catalyst.
This study reports the kinetics and mechanism of Fe(III)-catalyzed oxidative decarboxylation and deamination of a series of acyclic (α-aminoisobutyric acid, α-(methylamino)isobutyric acid, alanine, norvaline, and 2-aminobutyric acid) and cyclic (1-aminocyclopropane-1-carboxylic acid, 1-amino-1-cyclobutanecarboxylic acid, 1-aminocyclopentanecarboxylic acid, and 1-aminocyclohexanecarboxylicacid) amino acids using hydrogen peroxide, t-butyl hydroperoxide, iodosylbenzene, m-chloroperbenzoic acid, and peroxomonosulphate as oxidant in 75% DMF-25% water solvent mixture. Model complex [Fe(IV)O(SALEN)](•+) (SALENH2...
Source: Journal of Inorganic Biochemistry - May 23, 2013 Category: Biochemistry Authors: Góger S, Bogáth D, Baráth G, Simaan AJ, Speier G, Kaizer J Tags: J Inorg Biochem Source Type: research

Copper(II) interacting with the non-steroidal antiinflammatory drug flufenamic acid: Structure, antioxidant activity and binding to DNA and albumins.
Abstract Copper(II) complexes with the non-steroidal antiinflammatory drug flufenamic acid (Hfluf) in the presence of N,N-dimethylformamide (DMF) or nitrogen donor heterocyclic ligands (2,2'-bipyridylamine (bipyam), 1,10-phenanthroline (phen), 2,2'-bipyridine (bipy) or pyridine (py)) have been synthesized and characterized. The crystal structures of [Cu2(fluf)4(DMF)2], 1, and [Cu(fluf)(bipyam)Cl], 2, have been determined by X-ray crystallography. Density functional theory (DFT) (CAM-B3LYP/LANL2DZ/6-31G**) was employed to determine the structure of complex 2 and its analogues (complexes [Cu(fluf)(phen)Cl], 3, [Cu(f...
Source: Journal of Inorganic Biochemistry - May 23, 2013 Category: Biochemistry Authors: Tolia C, Papadopoulos AN, Raptopoulou CP, Psycharis V, Garino C, Salassa L, Psomas G Tags: J Inorg Biochem Source Type: research

The preparation of N-acetyl-Co(III)-microperoxidase-8 (NAcCoMP8) and its ligand substitution reactions: A comparison with aquacobalamin (vitamin B12a).
Abstract The synthesis of the Co(III) porphyrin octapeptide N-acetyl-Co(III) microperoxidase-8 (NAcCoMP8) is described. NAcCoMP8 provides a means of comparing and contrasting the chemistry of Co(III) porphyrins and corrins to assess the influence of the macrocycle. Log K values, and ΔH and ΔS, for the coordination of anionic (CN(-), N3(-), NO2(-), HSO3(-)) and neutral (pyridine, N-methylimidazole, methoxylamine and hydroxylamine) ligands by aquacobalamin (H2OCbl(+)) and NAcCoMP8 are reported. Anions bind more strongly to H2OCbl(+) than to NAcCoMP8 while the converse is true for the neutral ligands. Den...
Source: Journal of Inorganic Biochemistry - May 23, 2013 Category: Biochemistry Authors: Mathura S, Sannasy D, de Sousa AS, Perry CB, Navizet I, Marques HM Tags: J Inorg Biochem Source Type: research

Novel method for rapid copper chelation assessment confirmed low affinity of D-penicillamine for copper in comparison with trientine and 8-hydroxyquinolines.
This study was aimed at: 1) formation of an in vitro methodology for screening of copper chelators, and 2) detailed analysis of the interaction of copper with clinically used D-penicillamine (D-PEN), triethylenetetramine (trientine), experimentally tested 8-hydroxyquinolines, and the disodium salt of EDTA as a standard chelator. Methodology based on bathocuproinedisulfonic acid disodium salt (BCS), usable at (patho)physiologically relevant pHs (4.5-7.5), enabled assessment of both cuprous and cupric ions chelation and comparison of the relative affinities of the tested compounds for copper. In the case of potent chelators,...
Source: Journal of Inorganic Biochemistry - May 23, 2013 Category: Biochemistry Authors: Ríha M, Karlíčková J, Filipský T, Macáková K, Hrdina R, Mladěnka P Tags: J Inorg Biochem Source Type: research

Platinum-based drugs and proteins: reactivity and relevance to DNA adduct formation.
Abstract The mechanism of action of clinically used Pt-based drugs is through the formation of stable DNA adducts occurring at the nitrogen in position 7 of guanine (N7) and involving one or two spatially closed residues. Nevertheless, proteins can represent alternative targets since in particular sulfur groups, present in cysteine or methionine residues, can efficiently coordinate platinum. Here we have characterized the reactivity profile of cisplatin, transplatin and of two trans-platinum amine derivatives (TPAs) towards three different proteins, bovine α-lactalbumin (α-LA), hen egg lysozyme (LYS) a...
Source: Journal of Inorganic Biochemistry - May 1, 2013 Category: Biochemistry Authors: Pinato O, Musetti C, Farrell NP, Sissi C Tags: J Inorg Biochem Source Type: research

Complexes of ferriheme nitrophorin 4 with low-molecular weight thiol(ate)s occurring in blood plasma.
p M Abstract Nitrophorins are proteins occurring in the saliva of the blood-sucking insect Rhodnius prolixus to carry NO as a vasodilator and blood-coagulation inhibitor into the victim's tissue. It was suggested that the rate of NO release can be enhanced by the blood-plasma component L-cysteine [J.M.C.Ribeiro, Insect Biochem. Mol. Biol. 26 (1996) 899-905]. However, the mechanism of the reaction is not clear. In the attempt to exploit the reaction in detail, complexes of nitrophorin 4 (NP4) with the thiols 2-mercaptoethanol, L-cysteine, and L-homocysteine and with HS(-) were formed and characterized under anaerob...
Source: Journal of Inorganic Biochemistry - May 1, 2013 Category: Biochemistry Authors: He C, Nishikawa K, Erdem ÖF, Reijerse E, Ogata H, Lubitz W, Knipp M Tags: J Inorg Biochem Source Type: research

Impact of metal on the DNA photo-induced cleavage activity of a family of Phterpy complexes.
Abstract A family of Phterpy complexes, [Mn(Phterpy)2][N(CN)2]2·0.5H2O (1), [Fe(Phterpy)2](NO3)2 (2), [Ni(Phterpy)2](NO3)2 (3), [Ni(Phterpy)2]Cl2·10H2O (4), [Cd(Phterpy)2](NO3)2·2H2O (5) and [Zn(Phterpy)Cl2] (6) (Phterpy=4'-phenyl-2,2':6',2″-terpyridine), have been synthesized and structurally characterized, and their DNA binding and photo-induced DNA cleavage activities have been investigated. These complexes display binding propensity to the CT-DNA giving a relative order: 1>4>3, 5, 2, 6. Under dark or ambient lighting conditions, all complexes show no efficient DNA cleavage acti...
Source: Journal of Inorganic Biochemistry - May 1, 2013 Category: Biochemistry Authors: Chen GJ, Wang ZG, Kou YY, Tian JL, Yan SP Tags: J Inorg Biochem Source Type: research

Novel water-soluble (99m)Tc(I)/Re(I)-porphyrin conjugates as potential multimodal agents for molecular imaging.
Abstract The synthesis and characterization of two novel water soluble porphyrins with three meso pyridyl rings and one peripheral chelator - either a diethylenetriamine unit (4) or a bipyridyl fragment (8) - for binding to the {(99m)Tc(CO)3}(+) moiety is reported. In 8, despite the presence of a flexible and hydrophilic PEG-like linker that connects the bpy unit to the macrocycle, good water solubility was only obtained by methylation of the pyridyl N atoms that provided three extra positive charges. Furthermore, the water-soluble conjugates of 4 and 8 with either one fac-{Re(CO)3}(+) (9 and 10, respectively) or ...
Source: Journal of Inorganic Biochemistry - May 1, 2013 Category: Biochemistry Authors: Spagnul C, Alberto R, Gasser G, Ferrari S, Pierroz V, Bergamo A, Gianferrara T, Alessio E Tags: J Inorg Biochem Source Type: research

Comparative study of reaction of cobalamin and cobinamide with thiocyanate.
Abstract The interaction of Co(III) and Co(II) cobalamin (Cbl) and cobinamide (Cbi) with thiocyanate was examined with UV-vis and EPR spectra. S/N-linkage isomerism was explored on Co(III) and Co(II) Cbl and Cbi models using density functional theory (DFT; BP86, B3LYP). Performed calculations suggest the prevalence of isothiocyanato isomers over thiocyanato complexes on both Co(III) and Co(II) centers. The formation of Cbl(II) complex with thiocyanate was observed at high ligand concentrations which was proposed to be hexacoordinated. DFT data maintain the possibility of hexacoordinated Co(II) complexes with thioc...
Source: Journal of Inorganic Biochemistry - April 28, 2013 Category: Biochemistry Authors: Dereven'kov IA, Salnikov DS, Makarov SV, Surducan M, Silaghi-Dumitrescu R, Boss GR Tags: J Inorg Biochem Source Type: research

The second generation of iodido complexes: trans-[PtI2(amine)(amine')] bearing different aliphatic amines.
Quiroga AG Abstract The antitumoral potential for a series of platinum iodido complexes, all bearing the same aliphatic amines (first iodido complexes generation), was demonstrated in a previous study. Concretely, cis complexes were shown to have a peculiar and different reactivity compared to cisplatin with sulfur donors models and Cyt C. In this work we have synthesized and studied iodido complexes bearing different aliphatic amines in trans configuration (the second generation) to investigate their potential antitumor activity in a panel of cell lines. Their interaction with biological models such as pBR322 an...
Source: Journal of Inorganic Biochemistry - April 28, 2013 Category: Biochemistry Authors: Parro T, Medrano MA, Cubo L, Muñoz-Galván S, Carnero A, Navarro-Ranninger C, Quiroga AG Tags: J Inorg Biochem Source Type: research

Synthesis, chemical characterization and biological activity of new histone acetylation/deacetylation specific inhibitors: A novel and potential approach to cancer therapy.
Abstract Three new triorganotin(IV) complexes of valproic acid (vp1, Me3Sn-valproate; vp2, Bu3Sn-valproate; vp3, Ph3Sn-valproate) have been synthesized and investigated by spectroscopic and biological methods. An anionic, monodentate valproate ligand was observed, ester-like coordinating the tin atom on a tetra-coordinated, monomeric environment. The structures, though, can distort towards a penta-coordination, as a consequence of a long range O···Sn interaction. Crystallographic and NMR findings confirm this situation both in solid state and solution. Biological finding evidenced a clear cyto...
Source: Journal of Inorganic Biochemistry - April 25, 2013 Category: Biochemistry Authors: Pellerito O, Prinzivalli C, Foresti E, Sabatino P, Abbate M, Casella G, Fiore T, Scopelliti M, Pellerito C, Giuliano M, Grasso G, Pellerito L Tags: J Inorg Biochem Source Type: research