Selective interaction of Hpn-like protein with nickel, zinc and bismuth in vitro and in cells by FRET.
Abstract Hpn-like (Hpnl) is a unique histidine- and glutamine-rich protein found only in Helicobacter pylori and plays a role on nickel homeostasis. We constructed the fluorescent sensor proteins CYHpnl and CYHpnl_1-48 (C-terminal glutamine-rich region truncated) using enhanced cyan and yellow fluorescent proteins (eCFP and eYFP) as the donor-acceptor pair to monitor the interactions of Hpnl with metal ions and to elucidate the role of conserved Glu-rich sequence in Hpnl by fluorescence resonance energy transfer (FRET). CYHpnl and CYHpnl_1-48 exhibited largest responses towards Ni(II) and Zn(II) over other metals ...
Source: Journal of Inorganic Biochemistry - September 28, 2014 Category: Biochemistry Authors: Chang YY, Lai YT, Cheng T, Wang H, Yang Y, Sun H Tags: J Inorg Biochem Source Type: research

Introduction of a covalent histidine-heme linkage in a hemoglobin: A promising tool for heme protein engineering.
Abstract The hemoglobins of the cyanobacteria Synechococcus and Synechocystis (GlbNs) are capable of spontaneous and irreversible attachment of the b heme to the protein matrix. The reaction, which saturates the heme 2-vinyl by addition of a histidine residue, is reproduced in vitro by preparing the recombinant apoprotein, adding ferric heme, and reducing the iron to the ferrous state. Spontaneous covalent attachment of the heme is potentially useful for protein engineering purposes. Thus, to explore whether the histidine-heme linkage can serve in such applications, we attempted to introduce it in a test protein. ...
Source: Journal of Inorganic Biochemistry - September 28, 2014 Category: Biochemistry Authors: Rice SL, Preimesberger MR, Johnson EA, Lecomte JT Tags: J Inorg Biochem Source Type: research

Design, synthesis and SAR studies of novel 1,2-bis(aminomethyl)cyclohexane platinum(II) complexes with cytotoxic activity. Studies of interaction with DNA of iodinated seven-membered 1,4-diaminoplatinocycles.
re MT Abstract A selected library of nine novel platinum(II) complexes having differently functionalized 1,2-bis(aminomethyl)cyclohexane carrier ligands with a 1,4-diamino framework and iodides as labile ligands have been synthesized and evaluated in vitro for their tumor cell growth inhibitory activity, in front of one pair of human carcinoma cell lines A2780 and A2780cisR. These cell lines were chosen based on studying all the known main mechanisms of resistance of cisplatin. A2780cisR cells are resistant through a combination of reduced drug transport enhanced DNA repair/tolerance and elevated glutathione (GSH)...
Source: Journal of Inorganic Biochemistry - September 28, 2014 Category: Biochemistry Authors: Gay M, Montaña AM, Batalla C, Mesas JM, Alegre MT Tags: J Inorg Biochem Source Type: research

An investigation into the interactions of gold nanoparticles and anti-arthritic drugs with macrophages, and their reactivity towards thioredoxin reductase.
Abstract Gold(I) complexes are an important tool in the arsenal of established approaches for treating rheumatoid arthritis (RA), while some recent studies have suggested that gold nanoparticles (Au NPs) may also be therapeutically efficacious. These observations prompted the current biological studies involving gold(I) anti-RA agents and Au NPs, which are aimed towards improving our knowledge of how they work. The cytotoxicity of auranofin, aurothiomalate, aurothiosulfate and Au NPs towards RAW264.7 macrophages was evaluated using the MTT assay, with the former compound proving to be the most toxic. The extent of...
Source: Journal of Inorganic Biochemistry - September 28, 2014 Category: Biochemistry Authors: James LR, Xu ZQ, Sluyter R, Hawksworth EL, Kelso C, Lai B, Paterson DJ, de Jonge MD, Dixon NE, Beck JL, Ralph SF, Dillon CT Tags: J Inorg Biochem Source Type: research

2,6-Bis(2,6-diethylphenyliminomethyl)pyridine coordination compounds with cobalt(II), nickel(II), copper(II), and zinc(II): synthesis, spectroscopic characterization, X-ray study and in vitro cytotoxicity.
dijk J, Barba-Behrens N Abstract Coordination compounds with cobalt(II), nickel(II), copper(II) and zinc(II) and the ligand 2,6-bis(2,6-diethylphenyliminomethyl)pyridine (L) were synthesized and fully characterized by IR and UV-Vis-NIR spectroscopy, elemental analysis, magnetic susceptibility and X-ray diffraction for two representative cases. These novel compounds were designed to study their activity as anti-proliferative drugs against different human cancer cell lines. The tridentate ligand forms heptacoordinated compounds from nitrate metallic salts, where the nitrate acts in a chelating form to complete the s...
Source: Journal of Inorganic Biochemistry - September 22, 2014 Category: Biochemistry Authors: Martinez-Bulit P, Garza-Ortíz A, Mijangos E, Barrón-Sosa L, Sánchez-Bartéz F, Gracia-Mora I, Flores-Parra A, Contreras R, Reedijk J, Barba-Behrens N Tags: J Inorg Biochem Source Type: research

Syntheses, crystal structures and antioxidant study of Zn(II) complexes with morin-5'-sulfonic acid (MSA).
Abstract The study of modified synthetic procedure of water soluble morin-5'-sulfonic acid sodium salt (NaMSA) involving less aggressive chemicals and carried out at mild conditions was described. The NaMSA salt is a convenient source of anionic morin-5'-sulfonic ligand (MSA) in ion exchange reactions. The coordination ability of MSA ligand towards the zinc cations was investigated in aqueous solution and in solid state. Novel zinc complexes of morin-5'-sulfonate were obtained by a reaction of Zn(NO3)2 with morin-5'-sulfonate in water. Resulting compounds were characterized by single-crystal X-ray diffraction anal...
Source: Journal of Inorganic Biochemistry - September 18, 2014 Category: Biochemistry Authors: Pieniążek E, Kalembkiewicz J, Dranka M, Woźnicka E Tags: J Inorg Biochem Source Type: research

Mercury binding by methanobactin from Methylocystis strain SB2.
In this report, Hg binding by the structurally unique methanobactin from Methylocystis strain SB2 (mb-SB2) was examined and compared to mb-OB3b. Mb-SB2 is shown to bind the common forms of Hg found in aqueous environments, Hg(2+), Hg(CN)2 and CH3Hg(+). The spectral and thermodynamic properties of binding for each form of mercury differed. UV-visible absorption spectra suggested that Hg(2+) binds to both the oxazolone and imidazolone rings of mb-SB2, whereas CH3Hg(+) appeared to only bind to the oxazolone ring. Hg(CN)2 showed spectral properties between Hg(2+) and CH3Hg(+). Isothermal titration calorimetry (ITC) showed both...
Source: Journal of Inorganic Biochemistry - September 18, 2014 Category: Biochemistry Authors: Baral BS, Bandow NL, Vorobev A, Freemeier BC, Bergman BH, Herdendorf TJ, Fuentes N, Ellias L, Turpin E, Semrau JD, DiSpirito AA Tags: J Inorg Biochem Source Type: research

Mercury metallation of the copper protein azurin and structural insight into possible heavy metal reactivity.
Abstract Mercury(II) metallation of Pseudomonas aeruginosa azurin has been characterized structurally and biochemically. The X-ray crystal structure at 1.5Å of mercury(II) metallated azurin confirms the coordination of mercury at the copper binding active site and a second surface site. These findings are further validated by NMR, Matrix-assisted laser desorption/ionization spectrometry (MALDI), and UV-visible spectroscopic methods indicating copper displacement from the wild-type protein. Bioinformatic analysis has identified homologous human protein domains computationally, and compared them to the structu...
Source: Journal of Inorganic Biochemistry - September 16, 2014 Category: Biochemistry Authors: Zampino AP, Masters FM, Bladholm EL, Panzner MJ, Berry SM, Leeper TC, Ziegler CJ Tags: J Inorg Biochem Source Type: research

Ruthenium(II) polypyridyl complexes induce BEL-7402 cell apoptosis by ROS-mediated mitochondrial pathway.
Abstract A new ligand dmdppz and its four ruthenium(II) polypyridyl complexes [Ru(dmb)2(dmdppz)](ClO4)2 (1), [Ru(bpy)2(dmdppz)](ClO4)2 (2), [Ru(phen)2(dmdppz)](ClO4)2 (3) and [Ru(dmp)2(dmdppz)](ClO4)2 (4) (where dmb, bpy, phen, dmp and dmdppz stand for 4,4'-dimethyl-2,2'-bipyridine, 2,2'-bipyridine, 1,10-phenanthroline, 2,9-dimethyl-1,10-phenanthroline and 5,8-dimethoxylpyrido[3,2-a:2',3'-c]phenazine, respectively) have been synthesized and characterized. Their DNA binding behaviors show that the complexes bind to calf thymus DNA by intercalation. The complexes exhibit efficient photocleavage of pBR322 DNA on irra...
Source: Journal of Inorganic Biochemistry - September 16, 2014 Category: Biochemistry Authors: Jiang GB, Zheng X, Yao JH, Han BJ, Li W, Wang J, Huang HL, Liu YJ Tags: J Inorg Biochem Source Type: research

A new bis-3-hydroxy-4-pyrone as a potential therapeutic iron chelating agent. Effect of connecting and side chains on the complex structures and metal ion selectivity.
uk Z, Antonietta Zoroddu M, Peana M Abstract This work reports the synthesis, characterization and study of complex formation equilibria of the new ligand 6,6'-(2-(diethylamino)ethylazanediyl)bis(methylene)bis(5-hydroxy-2-hydroxymethyl-4H-pyran-4-one) with Fe(III), Al(III), Cu(II) and Zn(II). On the basis of previous encouraging results with tetradentate bis-kojic acid chelators, this ligand was designed to improve the pharmacokinetic properties: increase the solubility, neutral at physiological pH7.4, and enhancement of membrane crossing ability. Fe(III) and Al(III) complexation gave evidence of high metal-seques...
Source: Journal of Inorganic Biochemistry - September 16, 2014 Category: Biochemistry Authors: Nurchi VM, Crisponi G, Arca M, Crespo-Alonso M, Lachowicz JI, Mansoori D, Toso L, Pichiri G, Amelia Santos M, Marques SM, Niclós-Gutiérrez J, González-Pérez JM, Domínguez-Martín A, Choquesillo-Lazarte D, Szewczuk Z, Antonietta Zoroddu M, Peana M Tags: J Inorg Biochem Source Type: research

Synthesis, characterization, and antitumor activity of unusual pseudo five coordinate gold(III) complexes: Distinct cytotoxic mechanism or expensive ligand delivery systems?
Abstract Gold(III) complexes bearing bidentate ligands based on the 1,10-phenanthroline and 2,2'-bipyridine scaffolds have shown promising anticancer activity against a variety of tumor cell lines. In particular, our laboratory has previously found that a pseudo five coordinate gold(III) complex possessing the 2,9-di-sec-butyl-1,10-phenanthroline ligand {[((di-sec-butyl)phen)AuCl3]} exhibits antitumor activity against a panel of five different lung and head-neck tumor cell lines. However, the [((di-sec-butyl)phen)AuCl3] complex was determined to be less active than the free 2,9-di-sec-butyl-1,10-phenanthroline lig...
Source: Journal of Inorganic Biochemistry - September 6, 2014 Category: Biochemistry Authors: Olsen PM, Ruiz C, Lussier D, Le BK, Angel N, Smith M, Hwang CB, Khatib R, Jenkins J, Adams K, Getcher J, Tham F, Chen ZG, Wilson EH, Eichler JF Tags: J Inorg Biochem Source Type: research

Novel copper(II) complexes as new promising antitumour agents. A crystal structure of [Cu(1,10-phenanthroline-5,6-dione)2(OH2)(OClO3)](ClO4).
Abstract The cytotoxic properties of copper(II) complexes with 1,10-phenanthroline (phen) can be modified by substitution in the phen backbone. For this purpose, Cu(II) complexes with phen, 1,10-phenanthrolin-5,6-dione (phendione) and 1,10-phenanthrolin-5,6-diol (phendiol) have been synthesised and characterised. The crystal structure of [Cu(phendione)2(OH2)(OClO3)](ClO4) is discussed. The complex formation equilibria between Cu(II) and phen or phendione were studied by potentiometric measurements at 25 and 37°C in 0.1M ionic strength (NaCl). The antitumour activity of the compounds has been tested in vitro ag...
Source: Journal of Inorganic Biochemistry - September 4, 2014 Category: Biochemistry Authors: Pivetta T, Trudu F, Valletta E, Isaia F, Castellano C, Demartin F, Tuveri R, Vascellari S, Pani A Tags: J Inorg Biochem Source Type: research

Synthesis and biological assays on cancer cells of dinuclear gold complexes with novel functionalised di(N-heterocyclic carbene) ligands.
Abstract New dinuclear di(N-heterocyclic carbene) silver(I), gold(I) and gold(III) complexes have been synthesised and their antiproliferative effects towards various cancer cell lines have been screened. The di(N-heterocyclic carbene) ligands have a propylene linker between the carbene moieties and the imidazole backbone has been functionalised with a 1-benzyl- or 1-PEG-1,2,3-triazole ring (PEG=poly(ethylene glycol)) via a CuAAC (copper azido alkyne cycloaddition) reaction. The resulting gold(I) and gold(III) complexes display an antiproliferative activity superior to that of the unfunctionalised pristine complex...
Source: Journal of Inorganic Biochemistry - September 4, 2014 Category: Biochemistry Authors: Baron M, Bellemin-Laponnaz S, Tubaro C, Basato M, Bogialli S, Dolmella A Tags: J Inorg Biochem Source Type: research

Off-on-off pH luminescence switching and DNA binding properties of a free terpyridine-appended ruthenium complex.
Abstract A ruthenium(II) complex [Ru(bpy)2(HL(1))](ClO4)2·3H2O {bpy=2,2'-bipyridine and HL(1)=2-(2,6-di(pyridin-2-yl)pyridin-4-yl)-1H-imidazo[4,5-f][1,10]phenanthroline}, denoted as RuHL(1), was synthesized and characterized by elemental analysis, proton nuclear magnetic resonance spectroscopy, and mass spectrometry. The pH effects on the UV-visible (UV-vis) absorption and emission spectra and the luminescence lifetimes of RuHL(1) have been studied, and their ground- and excited-state acid ionization constants were derived. The complex was found to exhibit pH-induced "off-on-off" luminescence swit...
Source: Journal of Inorganic Biochemistry - September 3, 2014 Category: Biochemistry Authors: Zheng ZB, Kang SY, Yi X, Zhang N, Wang KZ Tags: J Inorg Biochem Source Type: research

Bioinorganic chemistry of synucleinopathies: Deciphering the binding features of Met motifs and His-50 in AS-Cu(I) interactions.
ez CO Abstract The aggregation of alpha-synuclein (AS) is a critical step in the etiology of Parkinson's disease (PD) and other neurodegenerative synucleinopathies. This process is selectively enhanced by copper in vitro and the interaction is proposed to play a potential role in vivo. Presently, the identity of the Cu(I) binding sites in AS and their relative affinities are under debate. In this work we have addressed unresolved details related to the structural binding specificity and affinity of Cu(I) to full-length AS. We demonstrated conclusively that: (i) the binding preferences of Cu(I) for the Met-binding ...
Source: Journal of Inorganic Biochemistry - September 3, 2014 Category: Biochemistry Authors: Miotto MC, Binolfi A, Zweckstetter M, Griesinger C, Fernández CO Tags: J Inorg Biochem Source Type: research

A hybrid antioxidizing and antibacterial material based on Ag-La2O3 nanocomposites.
Abstract The Ag-La2O3 hybrid nanoparticles were prepared by loading Ag nanoparticles on the surface of the La2O3 nanorods. The synthesis was a one-step process where sodium borohydride was used as a reducing agent to convert silver ions into silver nanoparticles, which were further deposited on the La2O3 nanorods. Moreover, they were found evenly dispersed upon the surface of La2O3 supports. The as-prepared Ag-La2O3 nanocomposites showed anti-oxidizing and significant antibacterial effect in vitro. Using the results from transmission electron microscope (TEM), the plausible mechanism was also proposed to explain t...
Source: Journal of Inorganic Biochemistry - August 27, 2014 Category: Biochemistry Authors: Wang K, Wu Y, Li H, Li M, Guan F, Fan H Tags: J Inorg Biochem Source Type: research

Decomposition of amino diazeniumdiolates (NONOates): Molecular mechanisms.
Abstract Although diazeniumdiolates (X[N(O)NO](-)) are extensively used in biochemical, physiological, and pharmacological studies due to their ability to release NO and/or its congeneric nitroxyl, the mechanisms of these processes remain obscure. In this work, we used a combination of spectroscopic, kinetic, and computational techniques to arrive at a quantitatively consistent molecular mechanism for decomposition of amino diazeniumdiolates (amino NONOates: R2N[N(O)NO](-), where R=N(C2H5)2 (1), N(C3H4NH2)2 (2), or N(C2H4NH2)2 (3)). Decomposition of these NONOates is triggered by protonation of their [NN(O)NO](-) ...
Source: Journal of Inorganic Biochemistry - August 23, 2014 Category: Biochemistry Authors: Shaikh N, Valiev M, Lymar SV Tags: J Inorg Biochem Source Type: research

Y(3+), La(3+), and some bivalent metals inhibited the opening of the Tl(+)-induced permeability transition pore in Ca(2+)-loaded rat liver mitochondria.
Abstract We showed earlier that diminution of 2,4-dinitrophenol (DNP)-stimulated respiration and increase of both mitochondrial swelling and electrochemical potential (ΔΨmito) dissipation in medium containing TlNO3 and KNO3 were caused by opening of Tl(+)-induced mitochondrial permeability transition pore (MPTP) in the inner membrane of Ca(2+)-loaded rat liver mitochondria. The MPTP opening was studied in the presence of bivalent metal ions (Sr(2+), Ba(2+), Mn(2+), Co(2+) and Ni(2+)), trivalent metal ions (Y(3+) and La(3+)), and ruthenium red. We found that these metal ions (except Ba(2+) and Co(2+)) as ...
Source: Journal of Inorganic Biochemistry - August 19, 2014 Category: Biochemistry Authors: Korotkov S, Konovalova S, Emelyanova L, Brailovskaya I Tags: J Inorg Biochem Source Type: research

Synthesis, DNA interaction and anticancer activity of copper(II) complexes with 4'-phenyl-2,2':6',2″-terpyridine derivatives.
Synthesis, DNA interaction and anticancer activity of copper(II) complexes with 4'-phenyl-2,2':6',2″-terpyridine derivatives. J Inorg Biochem. 2014 Aug 19;141C:17-27 Authors: Liang JW, Wang Y, Du KJ, Li GY, Guan RL, Ji LN, Chao H Abstract Three novel copper(II) complexes CuL(1)Cl2 (1) (L(1)=4'-(3-methoxyphenyl)-2,2':6'- 2″-terpyridine), CuL(2)Cl2 (2) (L(2)=4'-(4-methoxyphenyl)-2,2':6'-2″-terpyridine) and CuL(3)Cl2 (3) (L(3)=4'-(3,5-dimethoxyphenyl)-2,2':6'-2″-terpyridine) have been synthesized and characterized. Absorption spectral titration experiments, ethidium bromide displa...
Source: Journal of Inorganic Biochemistry - August 19, 2014 Category: Biochemistry Authors: Liang JW, Wang Y, Du KJ, Li GY, Guan RL, Ji LN, Chao H Tags: J Inorg Biochem Source Type: research

New heteronuclear gold(I)-platinum(II) complexes with cytotoxic properties: Are two metals better than one?
Abstract A series of mono- and heterodinuclear gold(I) and platinum(II) complexes with a new bipyridylamine-phosphine ligand have been synthesized and characterized. The X-ray structures of the ligand precursor 4-iodo-N,N-di(pyridin-2-yl)benzamide, and of one gold derivative are reported. All the complexes display antiproliferative properties in vitro in human cancer cells in the range of cisplatin or higher, which appear to correlate with compounds' uptake. Interestingly, studies of the interactions of the compounds with models of DNA indicate different mechanisms of actions with respect to cisplatin. The biologi...
Source: Journal of Inorganic Biochemistry - August 12, 2014 Category: Biochemistry Authors: Wenzel M, Bigaeva E, Richard P, Le Gendre P, Picquet M, Casini A, Bodio E Tags: J Inorg Biochem Source Type: research

Using methyl as substituted-radical in n-phen enhances the anticancer activities of [(DMF)Cu(n-phen)(NO3(-))2].
Abstract In order to seek better ligand for anticancer drug, we choose 1,10-phenanthroline (phen) and 2,9-dimethyl-1,10-phenanthroline (2,9-dmp) as predominant ligands, and synthetize two complexes:[(DMF)Cu(phen)(NO3)2] (1) and [(DMF)Cu(2,9-dmp)(NO3)2] (2) (DMF is dimethyl formamide). As for the five kinds of cancer cells, including A-549, Bel-7402, HCT-8, MDCK and L-1210 cells, our complexes showed higher inhibition ratio compared with anticancer drug 5-Fu (fluorouracil), ligand phenanthroline and Cu(NO3)2. It's worth noting that complex 2's anticancer activity is much more efficient than that of complex 1. This ...
Source: Journal of Inorganic Biochemistry - August 7, 2014 Category: Biochemistry Authors: Zhang B, Lu X, Wang G, Zhang W, Xia S, Chen Y Tags: J Inorg Biochem Source Type: research

Biological activity of a series of cisplatin-based aliphatic bis(carboxylato) Pt(IV) prodrugs: How long the organic chain should be?
Abstract The biological properties of a series of cisplatin-based Pt(IV) prodrug candidates, namely trans,cis,cis-[Pt(carboxylato)2Cl2(NH3)2], where carboxylato=CH3(CH2)nCOO(-) [(1), n=0; (2), n=2; (3), n=4; (4), n=6] having a large interval of lipophilicity are discussed. The stability of the complexes was tested in different pH conditions (i.e. from 1.0 to 9.0) to simulate the hypothetical conditions for an oral route of administration, showing a high stability (>90%). The transformation into their active Pt(II) metabolites was demonstrated in the presence of ascorbic acid, with a pseudo-first order kinetics,...
Source: Journal of Inorganic Biochemistry - August 7, 2014 Category: Biochemistry Authors: Zanellato I, Bonarrigo I, Colangelo D, Gabano E, Ravera M, Alessio M, Osella D Tags: J Inorg Biochem Source Type: research

A dimerization interface mediated by functionally critical residues creates interfacial disulfide bonds and copper sites in CueP.
Abstract CueP confers bacterial copper resistance in the periplasm, particularly under anaerobic conditions, through an unknown mechanism. The only available structure and limited solution data suggest that CueP forms noncovalent dimers in solution, whereas sequence conservation suggests important roles for three cysteines and two histidines as copper ligands. Here we report evidence of a dimerization equilibrium mediated by a newly identified interface of functional relevance, which occludes internal copper sites and disulfide bonds but allows for intra- and interchain disulfide bonding, an extensive disulfide re...
Source: Journal of Inorganic Biochemistry - August 7, 2014 Category: Biochemistry Authors: Abriata LA, Pontel LB, Vila AJ, Dal Peraro M, Soncini FC Tags: J Inorg Biochem Source Type: research

Mixed ligand copper(II) complexes of 2,9-dimethyl-1,10-phenanthroline: Tridentate 3N primary ligands determine DNA binding and cleavage and cytotoxicity.
Abstract A series of mononuclear mixed ligand copper(II) complexes of the type [Cu(L)(2,9-dmp)](ClO4)21-4, where L is a tridentate 3N ligand such as diethylenetriamine (L1) (1) or N-methyl-N'-(pyrid-2-yl-methyl)ethylenediamine (L2) (2) or di(2-picolyl)amine (L3) (3) or bis(pyrid-2-ylmethyl)-N-methylamine (L4) (4) and 2,9-dmp is 2,9-dimethyl-1,10-phenanthroline, has been isolated and characterized. The complexes 1 and 3 possess square-based pyramidal coordination geometry. Absorption spectral studies reveal that the intrinsic DNA binding affinity varies as 1>2>3>4. The higher DNA binding affinity of 1 aris...
Source: Journal of Inorganic Biochemistry - August 7, 2014 Category: Biochemistry Authors: Ganeshpandian M, Ramakrishnan S, Palaniandavar M, Suresh E, Riyasdeen A, Akbarsha MA Tags: J Inorg Biochem Source Type: research

Cinnamaldehyde and cuminaldehyde thiosemicarbazones and their copper(II) and nickel(II) complexes: A study to understand their biological activity.
Abstract This paper reports the synthesis and characterization of trans-cinnamaldehyde thiosemicarbazone (Htcin), cuminaldehyde thiosemicarbazone (Htcum) and their copper and nickel complexes. All the compounds, which on healthy cells (human fibroblasts) show a neglectable cytotoxicity, were screened in vitro in cell line U937 for their antileukemic activity. These compounds, in spite of their molecular similarity, present variegated behaviors. Htcin shows no inhibition activity in U935 cells, while both its metal complexes inhibit proliferation with IC50 at μM concentrations. The other ligand, Htcum, and its m...
Source: Journal of Inorganic Biochemistry - July 23, 2014 Category: Biochemistry Authors: Bisceglie F, Pinelli S, Alinovi R, Goldoni M, Mutti A, Camerini A, Piola L, Tarasconi P, Pelosi G Tags: J Inorg Biochem Source Type: research

Copper-binding properties of the BIR2 and BIR3 domains of the X-linked inhibitor of apoptosis protein.
We report the copper-binding properties of an XIAP construct containing the BIR2 and BIR3 domains. Absorption and emission spectroscopic measurements show that XIAP exhibits only a low-to-moderate affinity for Cu(II), but a strong affinity for Cu(I). Cu(I) is observed to bind at multiple sites within the BIR2 and BIR3 domains, including the CXXC motifs of the zinc structural sites and multiple BIR2 surface sites. Mutagenesis-based experiments reveal that surface cysteine residues mediate binding in the BIR2 domain and induce protein oligomerization under elevated copper concentrations. These results constitute the first sp...
Source: Journal of Inorganic Biochemistry - July 22, 2014 Category: Biochemistry Authors: Liang Y, Ewing PM, Laursen WJ, Tripp VT, Singh S, Splan KE Tags: J Inorg Biochem Source Type: research

Synthesis, anticancer activity and toxicity of a water-soluble 4S,5S-derivative of heptaplatin, cis-{Pt(II)[(4S,5S)-4,5-bis(aminomethyl)-2-isopropyl-1,3-dioxolane]·(3-hydroxyl-cyclobutane-1,1-dicarboxylate)}
Synthesis, anticancer activity and toxicity of a water-soluble 4S,5S-derivative of heptaplatin, cis-{Pt(II)[(4S,5S)-4,5-bis(aminomethyl)-2-isopropyl-1,3-dioxolane]·(3-hydroxyl-cyclobutane-1,1-dicarboxylate)} J Inorg Biochem. 2014 Jul 22;140C:126-130 Authors: Liu W, Jiang J, Xie C, Hou S, Quan H, Ye Q, Lou L Abstract A water-soluble 4S,5S-derivative of heptaplatin, cis-{Pt(II)[(4S,5S)-4,5-bis(aminomethyl)-2-isopropyl-1,3-dioxolane]·(3-hydroxyl-cyclobutane-1,1-dicarboxylate)} was synthesized. The anticancer activity and toxicity were evaluated by comparing its interaction with DNA, cytotox...
Source: Journal of Inorganic Biochemistry - July 22, 2014 Category: Biochemistry Authors: Liu W, Jiang J, Xie C, Hou S, Quan H, Ye Q, Lou L Tags: J Inorg Biochem Source Type: research

Effect of glucosamine conjugation to zinc(II) complexes of a bis-pyrazole ligand: Syntheses, characterization and anticancer activity.
Abstract The bis(3,5-dimethyl-1H- pyrazol-1yl)acetic acid (bdmpza) ligand was conjugated with tert-butyl-N-(2-aminoethyl) carbonate, methyl-2-amino-4-(methylthio)butanoate and 1,3,4,6-tetra-O-acetyl-β-d-glucosamine hydrochloride via amide coupling method to form three ligands L1-L3 which were then reacted with Zn(II) salts to form four zinc complexes (1-4). The complexes were characterized by (1)H NMR, (13)C NMR, electrospray ionization mass spectrometry (ESI-MS), FT-IR, CHN analyses. Complexes 1, 2 and 4 were also characterized by single crystal X-ray diffraction. It was found that Zn(II) salts could selecti...
Source: Journal of Inorganic Biochemistry - July 22, 2014 Category: Biochemistry Authors: Bhattacharyya S, Sarkar A, Dey SK, Mukherjee A Tags: J Inorg Biochem Source Type: research

The antimicrobial and antibiofilm activities of copper(II) complexes.
In this study we have analysed metal complexes of the general structure [M(IL)(AL)](2+) (where IL represents functionalised 1,10-phenanthrolines and AL represents 1S,2S- or 1R,2R-diaminocyclohexane) and [Cu(IL)3](2+). Antimicrobial activity was tested on a number of bacterial strains, showing that copper(II) compounds were active against both Gram-positive and Gram-negative bacteria, albeit that activity was generally higher for the former. The antibiofilm activity was then determined against a clinical isolate of meticillin-resistant Staphylococcus aureus (MRSA). Strikingly, the copper complexes tested showed significant ...
Source: Journal of Inorganic Biochemistry - July 22, 2014 Category: Biochemistry Authors: Beeton ML, Aldrich-Wright JR, Bolhuis A Tags: J Inorg Biochem Source Type: research

Spectroscopic, luminescence and in vitro biological studies of solid ketoprofen of heavier trivalent lanthanides and yttrium(III).
ach G Abstract Solid-state compounds of the general formulae [ML3] (M=Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, Y; L=ketoprofen) were synthesized and characterized using infrared, diffuse reflectance and luminescence spectroscopies. IR data suggested that the carboxylate group in ketoprofen is coordinated to the metals as a bidentate ligand. The triplet state energy level was determined using the Gd(3+) complex, which exhibited a ketoprofen blue luminescence when excited in the UV region. The compound containing Tb(3+) ion was sensitized by the ligand and emitted in the green region of the visible spectrum. On the other han...
Source: Journal of Inorganic Biochemistry - July 21, 2014 Category: Biochemistry Authors: Gálico DA, Lahoud MG, Davolos MR, Frem RC, Fraga-Silva TF, Venturini J, Arruda MS, Bannach G Tags: J Inorg Biochem Source Type: research

Behavior of the potential antitumor V(IV)O complexes formed by flavonoid ligands. 1. Coordination modes and geometry in solution and at the physiological pH.
Abstract The coordination modes and geometry assumed in solution by the potent antitumor oxidovanadium(IV) complexes formed by different flavonoids were studied by spectroscopic (Electron Paramagnetic Resonance, EPR) and computational (Density Functional Theory, DFT) methods. A series of bidentate flavonoid ligands (L) with increasing structural complexity was examined, which can involve (CO, O(-)) donors and formation of five- and six-membered chelate rings, or (O(-), O(-)) donors and five-membered chelate rings. The geometry corresponding to these coordination modes can be penta-coordinated, [VOL2], or cis-octah...
Source: Journal of Inorganic Biochemistry - July 19, 2014 Category: Biochemistry Authors: Sanna D, Ugone V, Lubinu G, Micera G, Garribba E Tags: J Inorg Biochem Source Type: research

Evaluation of the Debye temperature for iron cores in human liver ferritin and its pharmaceutical analogue, Ferrum Lek, using Mössbauer spectroscopy.
This study aimed to make a comparison of the Fe atom dynamics in the Ferrum Lek and ferritin iron cores by means of evaluation of the Debye temperature using the temperature dependence of the spectral center shift obtained with two different fitting procedures and the second order Doppler shift approach. The Debye temperature, evaluated as ΘD=502±24K for Ferrum Lek and ΘD=461±16K for human liver ferritin, demonstrated a very small difference in the Fe atom vibrations, reflecting a slightly smaller rigidity in the iron cores in human liver ferritin. PMID: 25086236 [PubMed - as supplied by publ...
Source: Journal of Inorganic Biochemistry - July 17, 2014 Category: Biochemistry Authors: Dubiel SM, Cieślak J, Alenkina IV, Oshtrakh MI, Semionkin VA Tags: J Inorg Biochem Source Type: research

Two structurally analogous ruthenium complexes as naked-eye and reversible molecular "light switch" for G-quadruplex DNA.
Two structurally analogous ruthenium complexes as naked-eye and reversible molecular "light switch" for G-quadruplex DNA. J Inorg Biochem. 2014 Jul 15;140C:64-71 Authors: Lu XH, Shi S, Yao JL, Gao X, Huang HL, Yao TM Abstract A pair of symmetrical furyl based ruthenium(II) complexes ([Ru(phen)2dpq-df](2+) (1) and [Ru(bpy)2dpq-df](2+) (2) (phen=1,10-phenanthroline, bpy=2,2'-bipyridine, dpq-df=dipyrido (3,2-a:2',3'-c) quinoxaline-difuran) have been prepared and characterized. The binding properties of both complexes toward G-quadruplex DNA have been investigated by fluorescence spectroscopy, U...
Source: Journal of Inorganic Biochemistry - July 15, 2014 Category: Biochemistry Authors: Lu XH, Shi S, Yao JL, Gao X, Huang HL, Yao TM Tags: J Inorg Biochem Source Type: research

Antitumor platinum(IV) derivatives of oxaliplatin with axial valproato ligands.
We report new anticancer prodrugs, platinum(IV) derivatives of oxaliplatin conjugated with valproic acid (VPA), a well-known drug having histone deacetylase inhibitory activity. Like most platinum(IV) derivatives, the cytotoxicity of the conjugates was lower in cell culture than that of oxaliplatin, but greater than those of its Pt(IV) derivative containing biologically inactive axial ligands in several cancer cell lines. Notably, these conjugates display activity in both cisplatin sensitive- and resistant tumor cells capable of both markedly enhanced accumulation in tumor cells and acting in a dual threat manner, concurre...
Source: Journal of Inorganic Biochemistry - July 15, 2014 Category: Biochemistry Authors: Novohradsky V, Zerzankova L, Stepankova J, Vrana O, Raveendran R, Gibson D, Kasparkova J, Brabec V Tags: J Inorg Biochem Source Type: research

Cyclopalladated and cycloplatinated benzophenone imines: Antitumor, antibacterial and antioxidant activities, DNA interaction and cathepsin B inhibition.
M, Calvet T Abstract The antitumor, antibacterial and antioxidant activity, DNA interaction and cathepsin B inhibition of cyclo-ortho-palladated and -platinated compounds [Pd(C,N)]2(μ-X)2 [X=OAc (1), X=Cl (2)] and trans-N,P-[M(C,N)X(PPh3)] [M=Pd, X=OAc (3), M=Pd, X=Cl (4), M=Pt, X=Cl (5)] are discussed [(C,N)=cyclo-ortho-metallated benzophenone imine]. The cytotoxicity of compound 5 has been evaluated towards human breast (MDA-MB-231 and MCF-7) and colon (HCT-116) cancer cell lines and that of compounds 1-4 towards the HCT-116 human colon cancer cell line. These cytotoxicities have been compared with those prev...
Source: Journal of Inorganic Biochemistry - July 15, 2014 Category: Biochemistry Authors: Albert J, D'Andrea L, Granell J, Pla-Vilanova P, Quirante J, Khosa MK, Calvis C, Messeguer R, Badía J, Baldomà L, Font-Bardia M, Calvet T Tags: J Inorg Biochem Source Type: research

Light-stable bis(norharmane)silver(I) compounds: Synthesis, characterization and antiproliferative effects in cancer cells.
Abstract Four different-anion Ag(I) compounds with the ligand norharmane (9H-Pyrido[3,4-b]indole; Hnor) and having the general formula [Ag(Hnor)2](anion) (anion=ClO4(-), NO3(-) and BF4(-)) [Ag(Hnor)2(MeCN)](PF6) are reported, and studied in detail regarding their coordination mode and in vitro antiproliferative effects. X-ray structural analysis revealed that the complex with the PF6(-) anion has a MeCN solvent molecule weakly coordinated to Ag(I), making the metal coordination T-shaped, while the other compounds present the classical linear Ag(I) coordination. The compounds showed certain cell growth inhibitory e...
Source: Journal of Inorganic Biochemistry - July 8, 2014 Category: Biochemistry Authors: Ahmad Khan R, Al-Farhan K, de Almeida A, Alsalme A, Casini A, Ghazzali M, Reedijk J Tags: J Inorg Biochem Source Type: research

A rhodium(III) complex inhibits LPS-induced nitric oxide production and angiogenic activity in cellulo.
This study demonstrates that kinetically inert rhodium(III) complexes may be potentially developed as effective anti-angiogenic agents. PMID: 25046384 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - July 8, 2014 Category: Biochemistry Authors: Liu LJ, Lin S, Chan DS, Vong CT, Hoi PM, Wong CY, Ma DL, Leung CH Tags: J Inorg Biochem Source Type: research

Proton pumping by an inactive structural variant of cytochrome c oxidase.
ski P Abstract The aa3-type cytochrome c oxidases (CytcOs) from e.g. Rhodobacter sphaeroides and Paracoccus denitrificans harbor two proton-transfer pathways. The K pathway is used for proton uptake upon reduction of the CytcO, while the D pathway is used after binding of O2 to the catalytic site. The aim of the present study was to determine whether or not CytcO in which the K pathway is blocked (by e.g. the Lys362Met replacement) is capable of pumping protons. The process can not be studied using conventional assays because the O2-reduction activity is too low when the K pathway is blocked. Consequently, proton ...
Source: Journal of Inorganic Biochemistry - July 1, 2014 Category: Biochemistry Authors: Svahn E, Faxén K, Gennis RB, Brzezinski P Tags: J Inorg Biochem Source Type: research

4th georgian bay international conference on bioinorganic chemistry.
PMID: 24928716 [PubMed - in process] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - June 20, 2014 Category: Biochemistry Authors: Stillman M, Kobayashi N, Frank Shaw C Tags: J Inorg Biochem Source Type: research

Copper(II) complexes of alloferon 1 with point mutations (H1A) and (H9A) stability structure and biological activity.
Abstract Mono- and polynuclear copper(II) complexes of the alloferon 1 with point mutations (H1A) A(1)GVSGH(6)GQH(9)GVH(12)G (Allo1A) and (H9A) H(1)GVSGH(6)GQA(9)GVH(12)G (Allo9A) have been studied by potentiometric, UV-visible, CD, EPR spectroscopic and mass spectrometry (MS) methods. To obtain a complete complex speciation different metal-to-ligand molar ratios ranging from 1:1 to 4:1 for Allo1A and to 3:1 for Allo9A were studied. The presence of the His residue in first position of the peptide chain changes the coordination abilities of the Allo9A peptide in comparison to that of the Allo1A. Imidazole-N3 atom o...
Source: Journal of Inorganic Biochemistry - June 2, 2014 Category: Biochemistry Authors: Matusiak A, Kuczer M, Czarniewska E, Rosiński G, Kowalik-Jankowska T Tags: J Inorg Biochem Source Type: research

Auranofin and related heterometallic gold(I)-thiolates as potent inhibitors of methicillin-resistant Staphylococcus aureus bacterial strains.
R, Contel M Abstract A series of new heterometallic gold(I) thiolates containing ferrocenyl-phoshines were synthesized. Their antimicrobial properties were studied and compared to that of FDA-approved drug, auranofin (Ridaura), prescribed for the treatment of rheumatoid arthritis. MIC in the order of one digit micromolar were found for most of the compounds against Gram-positive bacteria Staphylococcus aureus and CA MRSA strains US300 and US400. Remarkably, auranofin inhibited S. aureus, US300 and US400 in the order of 150-300nM. This is the first time that the potent inhibitory effect of auranofin on MRSA strains...
Source: Journal of Inorganic Biochemistry - May 28, 2014 Category: Biochemistry Authors: Hokai Y, Jurkowicz B, Fernández-Gallardo J, Zakirkhodjaev N, Sanaú M, Muth TR, Contel M Tags: J Inorg Biochem Source Type: research

Mono and dinuclear phosphinegold(I) sulfanylcarboxylates: Influence of nuclearity and substitution of PPh3 for PEt3 on cytotoxicity.
e;zquez-López EM Abstract Gold complexes of the type [Au(PEt3)(Hxspa)] were prepared by reacting triethylphosphinegold(I) chloride in ethanol/water (8:1) with the 3-(aryl)-2-sulfanylpropenoic acids H2xspa [x=p=3-phenyl-; f=3-(2-furyl)-; t=3-(2-thienyl)-; py=3-(2-pyridyl); Clp=3-(2-Chlorophenyl)-; -o-mp=3-(2-methoxyphenyl)-; -p-mp=3-(4-methoxyphenyl)-; -o-hp=3-(2-hydroxyphenyl)-; -p-hp=3-(4-hydroxyphenyl)-; -diBr-o-hp=3-(3,5-dibromo-2-hidroxyphenyl-); spa=2-sulfanylpropenoato] or 2-cyclopentylidene-2-sulfanylacetic acid (H2cpa) and KOH in a 1:1:1 mole ratio. The compounds were characterized by IR spectroscop...
Source: Journal of Inorganic Biochemistry - May 28, 2014 Category: Biochemistry Authors: Barreiro E, Casas JS, Couce MD, Sánchez A, Sánchez-Gonzalez A, Sordo J, Vázquez-López EM Tags: J Inorg Biochem Source Type: research

Structural diversity of polyoxomolybdate clusters along the three-fold axis of the molybdenum storage protein.
Abstract The molybdenum storage protein (MoSto) can store more than 100 Mo or W atoms as discrete polyoxometalate (POM) clusters. Here, we describe the three POM cluster sites along the threefold axis of the protein complex based on four X-ray structures with slightly different polyoxomolybdate compositions between 1.35 and 2Å resolution. In contrast to the Moα-out binding site occupied by an Mo3 cluster, the Moα-in and Moβ binding sites contain rather weak and non-uniform electron density for the Mo atoms (but clearly identifiable by anomalous data), suggesting the presence of POM cluster e...
Source: Journal of Inorganic Biochemistry - May 28, 2014 Category: Biochemistry Authors: Poppe J, Warkentin E, Demmer U, Kowalewski B, Dierks T, Schneider K, Ermler U Tags: J Inorg Biochem Source Type: research

Silver carboxylate metal-organic frameworks with highly antibacterial activity and biocompatibility.
Abstract Two novel Ag-based metal-organic frameworks (MOFs) [Ag2(O-IPA)(H2O)·(H3O)] (1) and [Ag5(PYDC)2(OH)] (2) were synthesized under the hydrothermal conditions using aromatic-carboxylic acids containing hydroxyl and pyridyl groups as ligands (HO-H2IPA=5-hydroxyisophthalic acid and H2PYDC=pyridine-3, 5-dicarboxylic acid). Single crystal X-ray diffraction indicated that two compounds exhibit three-dimensional frameworks constructed from different rod-shaped molecular building blocks. Both compounds favor slow release of Ag(+) ions leading to excellent and long-term antimicrobial activities towards Gram-ne...
Source: Journal of Inorganic Biochemistry - May 26, 2014 Category: Biochemistry Authors: Lu X, Ye J, Zhang D, Xie R, Bogale RF, Sun Y, Zhao L, Zhao Q, Ning G Tags: J Inorg Biochem Source Type: research

Ferrocene and (arene)ruthenium(II) complexes of the natural anticancer naphthoquinone plumbagin with enhanced efficacy against resistant cancer cells and a genuine mode of action.
Abstract A series of ferrocene and (arene)ruthenium(II) complexes attached to the naturally occurring anticancer naphthoquinones plumbagin and juglone was tested for efficacy against various cancer cell lines and for alterations in the mode of action. The plumbagin ferrocene and (p-cymene)Ru(II) conjugates 1c and 2a overcame the multi-drug drug resistance of KB-V1/Vbl cervix carcinoma cells and showed IC50 (72h) values around 1μM in growth inhibition assays using 3-(4,5-dimethyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT). They were further investigated for their influence on the cell cycle of KB-V1/Vbl and HCT...
Source: Journal of Inorganic Biochemistry - May 21, 2014 Category: Biochemistry Authors: Spoerlein-Guettler C, Mahal K, Schobert R, Biersack B Tags: J Inorg Biochem Source Type: research

Silver(I)/6-hydroxyiminolumazine compounds differently modify renin-angiotensin system-regulating aminopeptidases A and N in human neuroblastoma and glioma cells.
e;a F, Martínez-Martos JM, Moreno-Carretero MN Abstract We have described that local tissue renin-angiotensin-system (RAS) is involved in tumor growth in a rat model of experimental glioma in vivo, through the modification of their corresponding local proteolytic regulatory enzymes. Thus, we have found a time-dependent significant decrease in aminopeptidase N (APN) and a significant increase in aminopeptidase A (APA) activities concomitantly with tumor growth in tumor tissue whereas no changes were found in circulating aminopeptidase activities; we suggested that angiotensin peptides may play an essential s...
Source: Journal of Inorganic Biochemistry - May 21, 2014 Category: Biochemistry Authors: Ramírez-Expósito MJ, Mayas-Torres MD, Carrera-González MP, Jiménez-Pulido SB, Illán-Cabeza NA, Sánchez-Sánchez P, Hueso-Ureña F, Martínez-Martos JM, Moreno-Carretero MN Tags: J Inorg Biochem Source Type: research

Decavanadate in vitro and in vivo effects: facts and opinions.
Abstract This review covers recent advances in the understanding of the in vitro and in vivo effects of decavanadate, (V10O28)(6-), particularly in mitochondria. In vivo toxicological studies involving vanadium rarely account for the fact that under physiological conditions some vanadium may be present in the form of the decavanadate ion, which may behave differently from ortho- and metavanadates. It has for example been demonstrated that vanadium levels in heart or liver mitochondria are increased upon decavanadate exposure. Additionally, in vitro studies have shown that mitochondrial depolarization (IC50, 40 nM)...
Source: Journal of Inorganic Biochemistry - May 14, 2014 Category: Biochemistry Authors: Aureliano M, Ohlin CA Tags: J Inorg Biochem Source Type: research

New bioactive 2,6-diacetylpyridine bis(p-chlorophenylthiosemicarbazone) ligand and its Pd(II) and Pt(II) complexes: Synthesis, characterization, cytotoxic activity and DNA binding ability.
uza P Abstract Preparation and characterization of 2,6-diacetylpyridine bis((4)N-p-chlorophenylthiosemicarbazone) ligand, H2L, and its palladium(II) and platinum(II) complexes [PdL] and [PtL], is described. The molecular structure of the two new complexes has been determined by single crystal X-ray diffraction. The ligand acts as dianionic tetradentate donor coordinating to the metal center in a square planar geometry through the pyridine nitrogen atom and the azomethine nitrogen and thione sulfur atoms from one thiosemicarbazone arm, the fourth coordination position is occupied by the hydrazine nitrogen atom of t...
Source: Journal of Inorganic Biochemistry - May 9, 2014 Category: Biochemistry Authors: Matesanz AI, Hernández C, Souza P Tags: J Inorg Biochem Source Type: research

Tyrosine nitration in peptides by peroxynitrite generated in situ in a light-controlled platform: Effects of pH and thiols.
Abstract Peroxynitrite has been shown to play a critical role in inflammation and affords 3-nitrotyrosine as the hallmark product. The reported methods of generating this reactive nitrogen species in situ often fails to provide a high and steady flux of peroxynitrite resulting in poor yields of 3-nitrotyrosine. Herein we report a two-component peroxynitrite-generating platform in which this anion is produced in a biomimetic fashion and under the control of visible light. Incorporation of the nitric oxide- and superoxide-generating components in polymer matrices allows easy alterations of pH in the reaction wells o...
Source: Journal of Inorganic Biochemistry - May 9, 2014 Category: Biochemistry Authors: deBoer TR, Palomino RI, Idiga SO, Millhauser GL, Mascharak PK Tags: J Inorg Biochem Source Type: research

Complexing ability of (-)-cytisine - Synthesis, spectroscopy and crystal structures of the new copper and zinc complexes.
Abstract For the first time the NMR spectra of quinolizidine alkaloid with Cu(II) are studied. Structures of new complexes of (-)-cytisine with Cu(+2) and Zn(+2) cations are visualized, discussed in detail and characterized by spectroscopic methods: ESI-MS, NMR, UV-vis, EPR and crystallographic methods. In solution metal coordinates through the protonated nitrogen atoms of secondary amino groups (in piperidine ring) of cytisine molecule. While in solid state the most stable structures of the complexes are those in which the coordination of Cu(II) and Zn(II) salts is realized solely through the lactam carbonyl oxyg...
Source: Journal of Inorganic Biochemistry - May 9, 2014 Category: Biochemistry Authors: Przybył AK, Kubicki M, Jastrzab R Tags: J Inorg Biochem Source Type: research