Recent advances in platinum-based chemotherapeutics that exhibit inhibitory and targeted mechanisms of action.
Abstract Current platinum-based drugs used in chemotherapy, like cisplatin and its derivatives, are greatly limited due to side-effects and drug resistance. This has inspired the search for novel platinum-based drugs that deviate from the conventional mechanism of action seen with current chemotherapeutics. This review highlights recent advances in platinum(II) and platinum(IV)-based complexes that have been developed within the past six years. The platinum compounds explored within this review are those that display a more targeted approach by incorporating ligands that act on selected cellular targets within can...
Source: Journal of Inorganic Biochemistry - March 23, 2020 Category: Biochemistry Authors: Khoury A, Deo KM, Aldrich-Wright JR Tags: J Inorg Biochem Source Type: research

Synthesis, characterization, DNA binding and cytotoxicity studies of two novel Cu(II)-2-(2'-pyridyl) quinoxaline complexes.
Abstract Two novel copper (II) complexes, namely [Cu(2,2'-pq)(NO3)](NO3) (1) and [Cu(2,2'-pq)2(NO3)](NO3)·6H2O(2) where 2,2'-pq is 2-(2'-pyridyl quinoxaline) were synthesized and characterized by various spectral methods. Complex 2 characterized by single crystal X-ray diffraction showed a distorted trigonal bipyramidal geometry and crystallized together with an hexamer, (H2O)6 cluster, with R66(12) topology and chair conformation. The interaction of Calf Thymus DNA (CT-DNA) with 1 and 2 was investigated by UV-Visible absorption spectra, Viscosity, Cyclic Voltammetry, Fluorescence and Circular Dichroism (CD...
Source: Journal of Inorganic Biochemistry - March 23, 2020 Category: Biochemistry Authors: Lioli E, Psycharis V, Raptopoulou CP, Efthimiadou EK, Mitsopoulou CA Tags: J Inorg Biochem Source Type: research

Lysosome-targeted chemotherapeutics: Anticancer mechanism of N-heterocyclic carbene iridium(III) complex.
Abstract N-heterocyclic carbenes-modified half-sandwich iridium(III) complex [(η5-C5Me4C6H4C6H5)Ir(C^C)Cl]PF6 (C1) (where C^C is a N-heterocyclic carbene ligand) can effectively prevent the proliferation of human cervical cancer cells. Here, this study aims to investigate the in-deep anticancer effects of this complex on non-small cell lung cancer cells and explore the underlying molecular mechanism. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay showed that iridium(III) complex had potent cytotoxicity studies towards non-small cell lung cancer cells (A549), human lung squamous cells ...
Source: Journal of Inorganic Biochemistry - March 19, 2020 Category: Biochemistry Authors: Zhang J, Liu J, Liu X, Liu B, Song S, He X, Che C, Si M, Yang G, Liu Z Tags: J Inorg Biochem Source Type: research

DNA-binding of zinc(II) and nickel(II) salphen-like complexes extrapolated at 1  M salt concentration: Removing the ionic strength bias in physiological conditions.
DNA-binding of zinc(II) and nickel(II) salphen-like complexes extrapolated at 1 M salt concentration: Removing the ionic strength bias in physiological conditions. J Inorg Biochem. 2020 Mar 17;207:111064 Authors: Bonsignore R, Farine G, Migliore C, Gennaro G, Barone G Abstract The DNA-binding of two salphen-like metal complexes of nickel(II) (1) and zinc(II) (2) was investigated in different ionic strength solutions by absorption spectroscopy. The data analysis allowed us to obtain the values of their extrapolated DNA-binding constant in physiological conditions, with DNA-binding strength in the ...
Source: Journal of Inorganic Biochemistry - March 17, 2020 Category: Biochemistry Authors: Bonsignore R, Farine G, Migliore C, Gennaro G, Barone G Tags: J Inorg Biochem Source Type: research

Alternative modes of O2 activation in P450 and NOS enzymes are clarified by DFT modeling and resonance Raman spectroscopy.
Abstract The functions of heme proteins are modulated by hydrogen bonds (H-bonds) directed at the heme-bound ligands by protein residues. When the gaseous ligands CO, NO, or O2 are bound, their activity is strongly influenced by H-bonds to their atoms. These H-bonds produce characteristic changes in the vibrational frequencies of the heme adduct, which can be monitored by resonance Raman spectroscopy and interpreted with density functional theory (DFT) computations. When the protein employs a cysteinate proximal ligand, bound O2 becomes particularly reactive, the course of the reaction being controlled by H-bondin...
Source: Journal of Inorganic Biochemistry - March 13, 2020 Category: Biochemistry Authors: Soldatova AV, Spiro TG Tags: J Inorg Biochem Source Type: research

Nanostructured polymer assemblies stabilize photoactivatable anticancer ruthenium complexes under physiological conditions.
Abstract Photoactivatable ruthenium (Ru) complexes are promising compounds for anticancer phototherapy. They must be stable under physiological conditions before they are transported to cancer cells. In this paper, we systematically studied the stabilities of two Ru-containing block copolymers (Ru complexes as side group or in main chain, respectively) and their corresponding Ru complexes in different media, including saline, bovine serum albumin (BSA) solution, Dulbecco's Modified Eagle's Medium (DMEM, pH 6.5) and DMEM (pH 5.5) with 1.0 mM glutathione (GSH). Their stabilities were studied by monito...
Source: Journal of Inorganic Biochemistry - March 9, 2020 Category: Biochemistry Authors: Chen M, Sun W, Kretzschmann A, Butt HJ, Wu S Tags: J Inorg Biochem Source Type: research

A detailed analysis of the spin-crossover reaction of H2S binding to heme and the six-coordinated FeP(Im)-HS- porphyrin complex.
Abstract The potential energy surfaces of the H2S binding to iron-porphyrin (FeP) with the imidazole (Im) ligand via intersystem crossings are investigated by using density functional theory. The minimum energy intersystem crossing point (MEISCP) between the quintet and triplet states (MEISCPTQ) for the Fe(II)P(Im)-H2S complex is located at a Fe-S distance of 3.39 Å with only 1.1 kcal/mol above the quintet state minimum. The second spin-crossover point, where a change from the triplet to the singlet state occurs, comes at a much shorter Fe-S distance of 2.79 Å, and the MEISCPST is located at 3.7...
Source: Journal of Inorganic Biochemistry - February 25, 2020 Category: Biochemistry Authors: Ostojić BD, Schwerdtfeger P, Nakayama A, Hasegawa J, Đorđević DS Tags: J Inorg Biochem Source Type: research

Preparation, characterization and activity of CuZn and Cu2 superoxide dismutase mimics encapsulated in mesoporous silica.
ignorella S Abstract Encapsulation of three superoxide dismutase (SOD) functional mimics, [CuZn(dien)2(μ-Im)(ClO4)2]ClO4 (1), [Cu2(dien)2(μ-Im)(ClO4)2]ClO4 (2) (Im = imidazolate, dien = diethylenetriamine), and [CuZn(salpn)Cl2] (3) (H2salpn = 1,3-bis(salicylideneamino)propane) in mesoporous MCM-41 silica afforded three hybrid catalysts 1@MCM-41, 2@MCM-41 and 3@MCM-41. Spectroscopic and magnetic analyses of these materials confirmed the metal centers of the complexes keep the coordination sphere after insertion into the MCM-41 silica matrix. For the imidazolate-bridged complexes ...
Source: Journal of Inorganic Biochemistry - February 24, 2020 Category: Biochemistry Authors: Patriarca M, Daier V, Camí G, Rivière E, Hureau C, Signorella S Tags: J Inorg Biochem Source Type: research

Nitro-imidazole-based ruthenium complexes with antioxidant and anti-inflammatory activities.
, Lopes LGF Abstract Inflammation is a physiological process triggered in response to tissue damage, and involves events related to cell recruitment, cytokines release and reactive oxygen species (ROS) production. Failing to control the process duration lead to chronification and may be associated with the development of various pathologies, including autoimmune diseases and cancer. Considering the pharmacological potential of metal-based compounds, two new ruthenium complexes were synthesized: cis-[Ru(NO2)(bpy)2(5NIM)]PF6 (1) and cis-[RuCl(bpy)2(MTZ)]PF6 (2), where bpy = 2,2'-bipyridine, 5NIM = 5-nitroimi...
Source: Journal of Inorganic Biochemistry - February 24, 2020 Category: Biochemistry Authors: Sasahara GL, Gouveia Júnior FS, Rodrigues RO, Zampieri DS, Fonseca SGDC, Gonçalves RCR, Athaydes BR, Kitagawa RR, Santos FA, Sousa EHS, Nagao-Dias AT, Lopes LGF Tags: J Inorg Biochem Source Type: research

Density functional theory study of gold(III)-dithiocarbamate complexes with characteristic anticancer potentials.
In this study, we have provided a density functional theory (DFT) study of some gold(III)-dithiocarbamate complexes with characteristic anticancer potentials. DFT calculation of the reactivity and selectivity properties of these complexes with an enzyme template of thioredoxin reductase (TrxR) was carried out. The investigation proceeds with theoretical characterization of the selected compounds through spectroscopic analyses. IR and UV-vis analyses were carried out and the calculated values are comparable to experimental results. NMR assignment was determined for the gold compounds and the estimated theoretical chemical s...
Source: Journal of Inorganic Biochemistry - February 24, 2020 Category: Biochemistry Authors: Lawal MM, Lawal IA, Klink MJ, Tolufashe GF, Ndagi U, Kumalo HM Tags: J Inorg Biochem Source Type: research

Synthesis, chemical characterization and DNA interaction study of new diclofenac and ibuprofen zinc (II)-nicotinamide ternary complexes as cyclooxygenase inhibitor prototypes.
Abstract In the search for new drugs, strategies such as bioisosterism have been evidenced, in which the modification of molecules is already known to be active. Thus, metal complexes of known drugs have been highlighted, with examples of significant improvements in therapeutic efficacy. In this way, this work aimed at the synthesis of new zinc complexes with nonsteroidal anti-inflammatory drugs (NSAIDs), as well as the chemical characterization and the previous toxicity by cytotoxicity with Artemia salina, and evaluating the ability of these compounds to interact with DNA. As result, two new zinc II ternary compl...
Source: Journal of Inorganic Biochemistry - February 20, 2020 Category: Biochemistry Authors: Dos Santos PR, Pich CT, Back D, Smiderle F, Dumas F, Moura S Tags: J Inorg Biochem Source Type: research

Bioconjugates of Co(III) complexes with Schiff base ligands and cell penetrating peptides: Solid phase synthesis, characterization and antiproliferative activity.
Abstract In this work we synthesized a chelating Schiff base by a single condensation of salicylaldehyde with 3,4-diamino benzoic acid (1). This ligand was used further for complexation to CoCl2·6H2O under nitrogen. In the next step, three six-coordinate Co(III) complexes were synthesized by coordinating this complex with imidazole (2), 2-methyimidazole (3) and N-Boc-l-histidine methyl ester (4) (Boc: tert.-butoxycarbonyl) in axial positions with simultaneous oxidation of Co(II) to Co(III) under ambient environment. All Co(III) complexes were characterized by multinuclear NMR spectroscopy (1H, 13C and 59Co ...
Source: Journal of Inorganic Biochemistry - February 20, 2020 Category: Biochemistry Authors: Śmiłowicz D, Metzler-Nolte N Tags: J Inorg Biochem Source Type: research

Heteroleptic NiII complexes: Synthesis, structural characterization, computational studies and amoebicidal activity evaluation.
JC, Ruiz-Azuara L Abstract In this work, we present the synthesis, characterization, electrochemical studies, DFT calculations, and in vitro amoebicidal effect of seven new heteroleptic NiII coordination compounds. The crystal structures of [H2(pdto)](NO3)2 and [Ni(pdto)(NO3)]PF6 are presented, pdto = 2,2'-[1,2-ethanediylbis-(sulfanediyl-2,1-ethanediyl)]dipyridine. The rest of the compounds have general formulae: [Ni(pdto)(NN)](PF6) where N-N = 2,2'-bipyridine (bpy), 4,4'-dimethyl-2,2'-bipyridine (44dmbpy), 5,5'-dimethyl-2,2'-bipyridine (55dmbpy), 1,10-phenanthroline (phen), 4,7-dimethyl-1,10-phenanthrolin...
Source: Journal of Inorganic Biochemistry - February 19, 2020 Category: Biochemistry Authors: Hernández-Ayala LF, Toledano-Magaña Y, Ortiz-Frade L, Flores-Alamo M, Galindo-Murillo R, Reina M, García-Ramos JC, Ruiz-Azuara L Tags: J Inorg Biochem Source Type: research

Significance of conformation changes during the binding and release of chromium(III) from human serum transferrin.
Abstract Trivalent chromium has been proposed to be transported in vivo from the bloodstream to the tissues via endocytosis by transferrin (Tf), the major iron transport protein in the blood. While both Cr(III) binding and release from Tf have been proposed to be too slow to be physiologically relevant, recent kinetic studies under physiological conditions demonstrate that Cr(III) binding and release are sufficiently fast to occur during the time of the endocytosis cycle (circa 15 min). Consequently, the release of Cr(III) from human and bovine serum Tf has been examined under conditions mimicking an endosome...
Source: Journal of Inorganic Biochemistry - February 15, 2020 Category: Biochemistry Authors: Edwards KC, Kim H, Ferguson R, Lockart MM, Vincent JB Tags: J Inorg Biochem Source Type: research

Anionic versus neutral Pt(II) complexes: The relevance of the charge for human serum albumin binding.
Abstract The focus of this work is pointing out the different behavior of two structurally related Pt(II) complexes, the anionic cyclometalated NBu4[(Bzq)Pt(Thio)], 1 and the neutral [(Phen)Pt(Thio)], 2, (Bzq = benzo[h]quinoline, Phen = 1,10-phenantroline, Thio = 1,2-benzenedithiolate), on the interaction with human serum albumin (HSA), a key drug-delivery protein in the bloodstream. Being very limited the number of anionic Pt(II) complexes reported to date, this is a pioneering example of report on a protein-ligand interaction involving a negatively charged platinum compound. The stu...
Source: Journal of Inorganic Biochemistry - February 11, 2020 Category: Biochemistry Authors: Ricciardi L, Guzzi R, Rizzuti B, Ionescu A, Aiello I, Ghedini M, La Deda M Tags: J Inorg Biochem Source Type: research

Novel triorganotin complexes based on phosphonic acid ligands: Syntheses, structures and in vitro cytostatic activity.
Abstract Six novel organotin phosphonate complexes, [(Me3Sn)4(HL1)4]n1, [(Me3Sn)2(HL2)2]n2, [(Me3Sn)2L3(H2O)]n3, [(Ph3Sn)(HL1)]64, [(Ph3Sn)2L2]n5 and [(Ph3Sn)2L3]66, derived from phosphonic acid ligands [NaHL1 = 1-C10H7OPO2(OH)Na, H2L2 = 1-C10H7PO(OH)2, H2L3 = 2-C10H7PO(OH)2], have been synthesized and characterized by elemental analysis, FT-IR, NMR (1H, 13C, 31P and 119Sn) spectroscopy and X-ray crystallography. The structural analysis reveals that complexes 1 and 5 display 1D infinite zig-zag chain structures, and complex 2 shows 1D right-handed helical chain structure, while comple...
Source: Journal of Inorganic Biochemistry - February 8, 2020 Category: Biochemistry Authors: An BH, Zhang RF, Du XM, Li QL, Cheng S, Huang HL, Ma CL Tags: J Inorg Biochem Source Type: research

The structure of the oxidized state of cytochrome c oxidase - experiments and theory compared.
Abstract Cytochrome c oxidase (CcO), the terminal enzyme in the respiratory chain, reduces molecular oxygen to water. Experimental data on the midpoint potentials of the heme iron/copper active site cofactors do not match the overall reaction energetics, and are also in conflict with the observed efficiency of energy conservation in CcO. Therefore it has been postulated that the ferric/cupric intermediate (the oxidized state) exists in two forms. One form, labelled OH, is presumably involved during catalytic turnover, and should have a high CuB midpoint potential due to a metastable high energy structure. When no ...
Source: Journal of Inorganic Biochemistry - February 8, 2020 Category: Biochemistry Authors: Blomberg MRA Tags: J Inorg Biochem Source Type: research

The FeII(citrate) Fenton reaction under physiological conditions.
ein D Abstract The Fenton reaction of FeII(citrate) in the presence and absence of bicarbonate (HCO3-) is studied. It is found that the rate constant of the Fenton reaction (kobs) increases with increasing [citrate]. kobs also increase with increasing [HCO3-]; this effect is most significant at biological citrate concentrations. Methane and ethane gases are formed from (CH3)2SO when the Fenton reaction is carried out in the presence of large [citrate] due to the reaction of the citrate radical, (-2OC)CH2C(OH)(CO2-)CH(CO2-)/(-2OC)CH2C(O)(CO2-)CH2(CO2-) with (CH3)2SO. In the absence of citrate (CH3)2SO2 is the main ...
Source: Journal of Inorganic Biochemistry - February 4, 2020 Category: Biochemistry Authors: Illés E, Patra SG, Marks V, Mizrahi A, Meyerstein D Tags: J Inorg Biochem Source Type: research

Liposomes encapsulated iridium(III) polypyridyl complexes enhance anticancer activity in vitro and in vivo.
Abstract Three iridium(III) complexes [Ir(ppy)2(CPIP)](PF6) (Ir-1, ppy = 2-phenylpyridine, CPIP = 2-(4-chlorophenyl)-1H-imidazo[4,5-f][1,10]phenanthroline), [Ir(ppy)2(DCPIP)](PF6) (Ir-2, DCPIP = 2-(3,4-dichlorophenyl)-1H-imidazo[4,5-f][1,10]phenanthroline) and [Ir(ppy)2(TCPIP)](PF6) (Ir-3, TCPIP = 2,3,5-trichlorophenyl)-1H-imidazo[4,5-f][1,10]phenanthroline) were synthesized and characterized. The complexes Ir-1, Ir-2 and Ir-3 were encapsulated in liposomes to form Ir-1-Lipo, Ir-2-Lipo and Ir-3-Lipo. Morphology, size distribution, and zeta potential of liposomes were examine...
Source: Journal of Inorganic Biochemistry - January 31, 2020 Category: Biochemistry Authors: Bai L, Fei WD, Gu YY, He M, Du F, Zhang WY, Yang LL, Liu YJ Tags: J Inorg Biochem Source Type: research

Novel nickel(II), palladium(II), and platinum(II) complexes having a pyrrolyl-iminophosphine (PNN) pincer: Synthesis, crystal structures, and cytotoxic activity.
Abstract A pyrrolyl-iminophosphine (PNNH) which would act as a potential terdentate ligand has been prepared by Schiff base reaction. Complexes [M(PNN)X] (M = Ni; X = Cl (1), Pd; X = Cl (2), Br (3), I (4), M = Pt; X = Cl (5)) were prepared. The title complexes were characterized by various spectroscopic (IR, 1H, 13C, and 31P NMR) and elemental analyses. The molecular structures of 1, 2, and 5 have been established by single-crystal X-ray crystallography, demonstrating a distorted square planar geometry comprising two 5-membered metallacyclic rings. Complexes 1 and ...
Source: Journal of Inorganic Biochemistry - January 30, 2020 Category: Biochemistry Authors: Kim Y, Lee J, Son YH, Choi SU, Alam M, Park S Tags: J Inorg Biochem Source Type: research

The assembly and antitumor activity of lycium barbarum polysaccharide-platinum-based conjugates.
Abstract In this work, the new polysaccharide-platinum conjugates of 5-aminosalicylic acid modified lycium barbarum polysaccharide linking platinum compounds were designed in order to construct an anticancer metal drug delivery system. The multiple analysis methods were used to describe the chemical structure and physical properties of the polysaccharide-metal conjugates. The results showed that 5-aminosalicylic acid successfully acted as linker which was covalently bound between polysaccharide and platinum compound. The morphology and rheological properties of polysaccharide have been changed by the formation of ...
Source: Journal of Inorganic Biochemistry - January 24, 2020 Category: Biochemistry Authors: Wang Y, Han Q, Bai F, Luo Q, Wu M, Song G, Zhang H, Wang Y Tags: J Inorg Biochem Source Type: research

Synthesis, crystal structure and leishmanicidal activity of new trimethoprim Ru(III), Cu(II) and Pt(II) metal complexes.
riguetto AC Abstract Leishmaniasis is a parasitic disease caused by protozoa of the genus Leishmania, which has very limited treatment options and affects poor and underdeveloped populations. The current treatment is plagued by many complications, such as high toxicity, high cost and resistance to parasites; therefore, novel therapeutic agents are urgently needed. Herein, the synthesis, characterization and in vitro leishmanicidal potential of new complexes with the general formula [RuCl3(TMP)(dppb)] (1), [PtCl(TMP)(PPh3)2]PF6 (2) and [Cu(CH3COO)2(TMP)2]·DMF (3) (dppb = 1,4-bis(diphenylphosphino)b...
Source: Journal of Inorganic Biochemistry - January 23, 2020 Category: Biochemistry Authors: Silva GL, Dias JSM, Silva HVR, Teixeira JDS, De Souza IRB, Guimarães ET, de Magalhães Moreira DR, Soares MBP, Barbosa MIF, Doriguetto AC Tags: J Inorg Biochem Source Type: research

Iridium(III) coordination of N(6) modified adenine derivatives with aminoacid chains.
cute;zquez-López EM, Fiol JJ Abstract In this manuscript we report the preparation of three N6-aminoacid-adenine-derivatives: N-(7H-purin-6-yl)glycine·0.5H2O (N6-GlyAde), N-(7H-purin-6-yl)-β-alanine·1.5H2O (N6-β-AlaAde) and N-(7H-purin-6-yl)-γ-aminobutyric·2H2O (N6-GabaAde) and the synthesis and X-ray characterization of three Ir(III) NAMI-A derivatives (NAMI-A is [imidazoleH][trans-RuIIICl4(DMSO-κS)(imidazole)]) [trans-IrIIICl4(DMSO-κS)(N3-H)-(7H-purin-6-yl)glycine-κN9] (1), [trans-IrIIICl4(DMSO-κS)(N3-H)-(7H-purin-6-yl)-β-alanine-κN9]...
Source: Journal of Inorganic Biochemistry - January 18, 2020 Category: Biochemistry Authors: García-Raso A, Terrón A, Ortega-Castro J, Barceló-Oliver M, Lorenzo J, Rodríguez-Calado S, Franconetti A, Frontera A, Vázquez-López EM, Fiol JJ Tags: J Inorg Biochem Source Type: research

Cytotoxic Ag(I) and Au(I) NHC-carbenes bind DNA and show TrxR inhibition.
ani C Abstract A silver(I) and a gold(I) complex of the fluorescent N-heterocyclic carbenic (NHC) ligand 1-(9-anthracenylmethyl)-3-(3-trimethylsilyl-2-propynil)-benzimidazol-2-ylidene have been synthesized and characterized. These compounds show cytotoxicity in the micromolar range and higher antiproliferative properties than cisplatin (CDDP) against several tumour cell lines such as SW480 (colon), A549 (lung) and HepG2 (liver). Both metal complexes are successfully internalized by SW480 cells being the silver compound the most accumulated. Subsequently, they were evaluated as inhibitors of the selenoenzyme Thiore...
Source: Journal of Inorganic Biochemistry - January 15, 2020 Category: Biochemistry Authors: Guarra F, Busto N, Guerri A, Marchetti L, Marzo T, García B, Biver T, Gabbiani C Tags: J Inorg Biochem Source Type: research

Multiple drug binding modes in Mycobacterium tuberculosis CYP51B1.
Abstract The Mycobacterium tuberculosis (Mtb) genome encodes 20 different cytochrome P450 enzymes (CYPs), many of which serve essential biosynthetic roles. CYP51B1, the Mtb version of eukaryotic sterol demethylase, remains a potential therapeutic target. The binding of three drug fragments containing nitrogen heterocycles to CYP51B1 is studied here by continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) techniques to determine how each drug fragment binds to the heme active-site. All three drug fragments form a mixture of complexes, some of which retain the axial water ligand from the resting stat...
Source: Journal of Inorganic Biochemistry - January 13, 2020 Category: Biochemistry Authors: Lockart MM, Butler JT, Mize CJ, Fair MN, Cruce AA, Conner KP, Atkins WM, Bowman MK Tags: J Inorg Biochem Source Type: research

Tau/A β chimera peptides: Evaluating the dual function of metal coordination and membrane interaction in one sequence.
In this study, we assess the amyloidogenic propensity and membrane-damage effects, either in the presence or in absence of metal ions, of two newly synthesized bifunctional peptides. These were designed to comprise a metal chelating N-terminus region derived from Tau protein namely the Tau9-16 (EVMEDHAG) or Tau26-33 (QGGYTMHQ) sequences, merged with the C-terminal hydrophobic region analogous to the Amyloid beta (Aβ) 16-20 aminoacid sequence KLVFF (KL). Comparative circular dichroism or fluorescence experiments were carried out to look at the peptide conformation, fibril formation and membrane affinity of Tau9-16KL an...
Source: Journal of Inorganic Biochemistry - January 11, 2020 Category: Biochemistry Authors: Sciacca MFM, Di Natale G, Tosto R, Milardi D, Pappalardo G Tags: J Inorg Biochem Source Type: research

Ditopic binuclear copper(II) complexes for DNA cleavage.
an-Gómez D Abstract Herein we present the synthesis of two ligands containing two di(2-picolyl)amine (DPA) units linked by either a 1,1'-(pyridine-2,6-diyl)bis(3-ethylurea) (L1) or a 1,1'-(1,3-phenylene)bis(3-ethylurea) (L2) spacer. The corresponding binuclear CuII and ZnII complexes were prepared and isolated. The X-ray structures of the L1 ligand and the [Cu2L1Cl2]2+ complex evidence an unusual cis/trans conformation of one of the urea groups stabilized by an intramolecular hydrogen bond with the nitrogen atom of the pyridyl spacer. The CuII complexes form rather strong ternary complexes with phosphorylat...
Source: Journal of Inorganic Biochemistry - January 11, 2020 Category: Biochemistry Authors: Carreira-Barral I, Riopedre-Fernández M, de Blas A, Mosquera J, Vázquez ME, Platas-Iglesias C, Esteban-Gómez D Tags: J Inorg Biochem Source Type: research

Dual functions of iridium(III) 2-phenylpyridine complexes: Metastasis inhibition and lysosomal damage.
Abstract Six N-phenylcarbazole/triphenylamine-appended half-sandwich iridium(III) 2-phenylpyridine complexes ([(η5-Cp*)Ir(C^N)Cl]) were prepared and characterized. Compared with cisplatin, these complexes exhibited potential antitumor activity against A549 and HeLa tumor cells, with IC50 values (half-maximum inhibitory concentration) that changed from 2.8 ± 0.8 μM to 39.5 ± 2.7 μM, and could block the migration of tumor cells. These complexes also effectively bound to protein (binding constant: ~104 M-1) and were transported through serum proteins, catalyz...
Source: Journal of Inorganic Biochemistry - January 8, 2020 Category: Biochemistry Authors: Liu X, Chen S, Ge X, Zhang Y, Xie Y, Hao Y, Wu D, Zhao J, Yuan XA, Tian L, Liu Z Tags: J Inorg Biochem Source Type: research

Biological sulfur in the blood cells of Ascidia ceratodes: XAS spectroscopy and a cellular-enzymatic hypothesis for vanadium reduction in the ascidians.
Abstract Two samples of living blood cells and of cleared blood plasma from the Phlebobranch tunicate Ascidia ceratodes from Bodega Bay, California, and one of fresh Henze solution from A. ceratodes of Monterey Bay, California, have been examined using sulfur K-edge x-ray absorption spectroscopy (XAS). Biological sulfur included sulfate esters, sulfate and bisulfate ions, benzothiazole, thianthrene, epi-sulfide, thiol and disulfide. Glutathione dominated reduced sulfur, from which an average intracellular Voltage of -0.21 V was calculated. Sulfate-bisulfate ratios yielded blood cell pH values of 2.0 and 2.8. Total...
Source: Journal of Inorganic Biochemistry - January 7, 2020 Category: Biochemistry Authors: Frank P, Carlson RMK, Carlson EJ, Hedman B, Hodgson KO Tags: J Inorg Biochem Source Type: research

Oxidative damage under As3+ and/or Cu2+ stress leads to apoptosis and autophagy and may be cross-talking with mitochondrial disorders in bursa of Fabricius.
In conclusion, apoptosis and autophagy of BF induced by As3+ and Cu2+ and mitochondrial disorder are closely related to the collapse of antioxidant system, and their connections are inseparable. Our results provide a reference for environmental risk prevention and selection of poultry feed additives and pesticides to avoid the health risks caused by As3+ and Cu2+ exposure. PMID: 31945648 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - January 7, 2020 Category: Biochemistry Authors: Guo M, Wang Y, Zhao H, Mu M, Yang X, Fei D, Liu Y, Zong H, Xing M Tags: J Inorg Biochem Source Type: research

Cu(II) coordination to His-containing linear peptides and related branched ones: Equalities and diversities.
Abstract The two branched peptides (AAHAWG)4-PWT2 and (HAWG)4-PWT2 where synthesized by mounting linear peptides on a cyclam-based scaffold (PWT2), provided with four maleimide chains, through a thio-Michael reaction. The purpose of this study was primarily to verify if the two branched ligands had a Cu(II) coordination behavior reproducing that of the single-chain peptides, namely AAHAWG-NH2, which bears an Amino Terminal Cu(II)- and Ni(II)-Binding (ATCUN) Motif, and HAWG-NH2, which presents a His residue as the N-terminal amino acid, in a wide pH range. The study of Cu(II) binding was performed by potentiometric...
Source: Journal of Inorganic Biochemistry - January 3, 2020 Category: Biochemistry Authors: Perinelli M, Guerrini R, Albanese V, Marchetti N, Bellotti D, Gentili S, Tegoni M, Remelli M Tags: J Inorg Biochem Source Type: research

Exploring the interactions of iron and zinc with the microtubule binding repeats R1 and R4.
Abstract The dyshomeostasis of copper, iron and zinc ions in pathological conditions, which are critically involved in many brain activities, may result in an accumulation of them in the brain that has been reported for the patients with Alzheimer's disease. Conformational change is one of the consequences of metal-peptide interaction as we observed for the interaction of the Cu2+ with microtubule binding repeats of tau protein, which ultimately cause peptide aggregation. Herein, we show that interaction of Zn2+, Fe2+, and Fe3+ with full-length tau peptide R1 (tau244-274) and R4 (tau337-368), the first and fourth ...
Source: Journal of Inorganic Biochemistry - December 31, 2019 Category: Biochemistry Authors: Ahmadi S, Wu B, Song R, Zhu S, Simpson A, Wilson DJ, Kraatz HB Tags: J Inorg Biochem Source Type: research

Formation and kinetic studies of manganese(IV)-oxo porphyrins: Oxygen atom transfer mechanism of sulfide oxidations.
Abstract Visible light irradiation of photo-labile porphyrin-manganese(III) chlorates or bromates (2) produced manganese(IV)-oxo porphyrins [MnIV(Por)(O)] (Por = porphyrin) (3) in three porphyrin ligands. The same oxo species 3 were also formed by chemical oxidation of the corresponding manganese(III) precursors (1) with iodobenzene diacetate, i.e. PhI(OAc)2. The systems under study include 5,10,15,20-tetra(pentafluorophenyl)porphyrin‑manganese(IV)-oxo (3a), 5,10,15,20-tetra(2,6-difluorophenyl)porphyrin‑manganese(IV)-oxo (3b), and 5,10,15,20-tetramesitylporphyrin‑manganese(IV)-oxo (3c). As expected...
Source: Journal of Inorganic Biochemistry - December 31, 2019 Category: Biochemistry Authors: Klaine S, Bratcher F, Winchester CM, Zhang R Tags: J Inorg Biochem Source Type: research

Metal complexes for mitochondrial bioimaging.
Abstract Mitochondria are essential organelles in eukaryotic cells, containing various signaling molecules and important enzymes associated with cell growth, death, and proliferation. The visualization of mitochondria and their biochemistry with confocal microscopy, fluorescence (phosphorescence) lifetime microscopy (FLIM, PLIM), and super-resolution microscopy has therefore been of great interest in recent years. In particular, transition metal complexes have emerged as excellent mitochondria-targeting probes, due to their high photostabilities, large Stokes shifts, tunable chemical structures and long luminescen...
Source: Journal of Inorganic Biochemistry - December 30, 2019 Category: Biochemistry Authors: Guan R, Xie L, Rees TW, Ji L, Chao H Tags: J Inorg Biochem Source Type: research

A trans-dichloridoplatinum(II) complex of a monodentate nitrogen mustard: Synthesis, stability and cytotoxicity studies.
Abstract A trans-dichloridoplatinum(II) complex, trans-[PtIICl2(L)(DMSO)] (1) of a monodentate nitrogen mustard, bis(2-chloroethyl)amine (L), was synthesized by the reaction of cis-[PtIICl2(DMSO)2] &L.HCl in presence of Et3N. 1 was characterised by NMR, FT-IR and elemental analysis. L is unstable in aqueous solution while 1 displayed moderate stability. In aqueous buffer solution of pD 7.4, 1 starts to loose L slowly upon dissolution and even after 48 h there is still intact/aquated complex present in solution. 1 interacts with the model nucleobase 9-ethyl guanine. The ligand L was non-toxic against MCF-7...
Source: Journal of Inorganic Biochemistry - December 28, 2019 Category: Biochemistry Authors: Karmakar S, Chatterjee S, Purkait K, Mukherjee A Tags: J Inorg Biochem Source Type: research

Anticancer and antimetastatic activity of copper(II)-tropolone complex against human breast cancer cells, breast multicellular spheroids and mammospheres.
;n IE Abstract The goal of this work was to display the anticancer and antimetastatic activity of a copper(II) with tropolone (trp), complex [Cu(trp)2] toward human breast cancer cells in monolayer (2D) and spheroids (3D). Cytotoxicity assays against MCF7 (IC50(complex) = 5.2 ± 1.8 μM, IC50(CDDP) = 19.3 ± 2.1 μM) and MDA-MB-231 (IC50(complex) = 4.0 ± 0.2 μM, IC50(CDDP) = 27.0 ± 1.9 μM) demonstrate that [Cu(trp)2] exert greater antitumor potency than cisplatin (CDDP) on 2D and ...
Source: Journal of Inorganic Biochemistry - December 26, 2019 Category: Biochemistry Authors: Balsa LM, Ruiz MC, Santa Maria de la Parra L, Baran EJ, León IE Tags: J Inorg Biochem Source Type: research

The influence of land-use on tropical soil chemical characteristics with emphasis on aluminium.
PD, Lojka B, Němeček K, Borůvka L, Drábek O Abstract Composition of soil vegetation cover and land management directly influences the cycling of chemical elements and is a key factor for soil biogeochemistry and also Al behaviour. Moreover, Al is an important factor limiting the growth of cultural plants. Our results are based on long-term observations of soils translocated from selected small areas of eight 1 ha plots of different land-use gradient, with identical geological, climatic and geographical conditions, located in the North of Congo Basin (near Mbalmayo, Cameroon). The plots are established ...
Source: Journal of Inorganic Biochemistry - December 23, 2019 Category: Biochemistry Authors: Tejnecký V, Křížová P, Penížek V, Maňourová A, Sillam-Dussès D, Šobotník J, Akama PD, Lojka B, Němeček K, Borůvka L, Drábek O Tags: J Inorg Biochem Source Type: research

Synthesis, characterization and albumin binding capabilities of quinizarin containing ternary cobalt(III) complexes.
cute;A, Buglyó P Abstract Four Co(III) ternary complexes with the composition of [(Co(4 N))2(quin)](ClO4)4 or [(Co(4 N))2(quinS)](ClO4)3, where 4 N = tris(2-aminoethyl)amine (tren) or tris(2-pyridylmethyl)amine (tpa), quinH2 = quinizarin (1,4-dihydroxy-9,10-anthraquinone), quinSH3 = quinizarin-2-sulfonic acid (1,4-dihydroxy-9,10-anthraquinone-2-sulfonic acid), were synthesized, characterized and their human serum albumin (HSA) binding capabilities were also tested. The complexes can be considered as likely chaperons of quinizarins which are structural models for ...
Source: Journal of Inorganic Biochemistry - December 17, 2019 Category: Biochemistry Authors: Kozsup M, Dömötör O, Nagy S, Farkas E, Enyedy ÉA, Buglyó P Tags: J Inorg Biochem Source Type: research

Urease inhibition studies of six Ni(II), Co(II) and Cu(II) complexes with two sexidentate N2O4-donor bis-Schiff base ligands: An experimental and DFT computational study.
Abstract Six novel complexes, [Ni(C36H34N2O10)]·2.25CH3OH·0.5C4H10O (1), [Co(C36H34N2O10)] (2), [Cu(C36H34N2O10)]·2CH3OH (3), [Ni(C36H32N2O8Cl2)]·2CH3OH (4), [Co(C36H32N2O8Cl2)]·4CH3OH (5) and [Cu(C36H32N2O8Cl2)]·2CH3OH (6) with two sexidentate N2O4-donor bis-Schiff base ligands (C36H34N2O10 = 1,2-bis(2-methoxy-6-formylphenoxy)ethane-l-tyrosine; C36H32N2O8Cl2 = 1,2-bis(2-methoxy-6-formylphenoxy)ethane-l-4-chlorophenylalanine) have been synthesized and structurally characterized. Theoretical calculation of the six complexes was carried out by density fun...
Source: Journal of Inorganic Biochemistry - December 16, 2019 Category: Biochemistry Authors: Wang H, Xu C, Zhang X, Zhang D, Jin F, Fan Y Tags: J Inorg Biochem Source Type: research

Aluminium alters mineral composition and polyphenol metabolism in leaves of tea plants (Camellia sinensis).
In conclusion, low pH and enhanced Al availability to tea plants have a strong influence on the polyphenolic pattern of tealeaves and therefore may alter both the leaves' antioxidant properties and their ability to bind Al and Fe in non-toxic form. PMID: 31862583 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - December 12, 2019 Category: Biochemistry Authors: Tolrà R, Martos S, Hajiboland R, Poschenrieder C Tags: J Inorg Biochem Source Type: research

Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles.
Abstract The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV-Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorptio...
Source: Journal of Inorganic Biochemistry - December 11, 2019 Category: Biochemistry Authors: Laban B, Ralević U, Petrović S, Leskovac A, Vasić-Anićijević D, Marković M, Vasić V Tags: J Inorg Biochem Source Type: research

Gallium-68 and scandium-44 labelled radiotracers based on curcumin structure linked to bifunctional chelators: Synthesis and characterization of potential PET radiotracers.
i M Abstract Curcumin metal complexes showed widespread applications in medicine and can be exploited as a lead structure for developing new tracers for nuclear medicine application. Herein, the synthesis, chemical characterization and radiolabelling with gallium-68 and scandium-44 of two new targeting vectors based on curcumin scaffolds and linked to the chelators 1,4,7-triazacyclononane,1-glutaric acid-4,7-acetic acid (NODAGA) and 1,4-bis(carboxymethyl)-6-[bis(carboxymethyl)]amino-6-methylperhydro-1,4-diazepine (AAZTA) are reported. Synthesis of the precursors could be achieved with a 13% and 11% yield and radio...
Source: Journal of Inorganic Biochemistry - December 5, 2019 Category: Biochemistry Authors: Orteca G, Sinnes JP, Rubagotti S, Iori M, Capponi PC, Piel M, Rösch F, Ferrari E, Asti M Tags: J Inorg Biochem Source Type: research

Structural zinc binding sites shaped for greater works: Structure-function relations in classical zinc finger, hook and clasp domains.
Abstract Metal ions are essential elements present in biological systems able to facilitate many cellular processes including proliferation, signaling, DNA synthesis and repair. Zinc ion (Zn(II)) is an important cofactor for numerous biochemical reactions. Commonly, structural zinc sites demonstrate high Zn(II) affinity and compact architecture required for sequence-specific macromolecule binding. However, how Zn(II)-dependent proteins fold, how their dissociation occurs, and which factors modulate zinc protein affinity as well as stability remains not fully understood. The molecular rules governing precise regula...
Source: Journal of Inorganic Biochemistry - December 5, 2019 Category: Biochemistry Authors: Padjasek M, Kocyła A, Kluska K, Kerber O, Tran JB, Krężel A Tags: J Inorg Biochem Source Type: research

Triplet of cysteines - Coordinational riddle?
In this study, the interaction of Zn2+, Cd2+ and Ni2+ ions with four peptides containing cysteine triplet motif were studied by potentiometric and spectroscopic methods. The main goal of this research was to answer the question how effectively three thiols, each being next to other, are able to bind single metal ion. Two of peptides contain additional, fourth cysteine residue, separated from triplet by two and three other amino acid residues. As results show, all three cysteine residues in the CCC motif are able to participate in the coordination of the metal ion (Cd2+, Zn2+). Except cysteine thiol groups, amide nitrogen a...
Source: Journal of Inorganic Biochemistry - December 5, 2019 Category: Biochemistry Authors: Krzywoszyńska K, Swiątek-Kozłowska J, Potocki S, Ostrowska M, Kozłowski H Tags: J Inorg Biochem Source Type: research

Ternary copper(II) complex of 5-hydroxytryptophan and 1,10-phenanthroline with several pharmacological properties and an adequate safety profile.
We report the synthesis and biological evaluation of a ternary copper complex, [Cu(5HTP)(phen)(H2O)](NO3).2H2O, with the antioxidant agent 5-hydroxytryptophan (5-HTP) and phenanthroline (phen, added to improve its lipophilicity and membrane transport). The crystal structure of the complex was determined by X-ray diffraction methods. The complex showed antioxidant, antimicrobial, antitumor and antimetastatic properties with an adequate safety profile. The interaction of the metal with phen promotes cellular copper accumulation and cytotoxicity on human lung A549 cell line (IC50 = 3.6 μM). Furthermore, the ...
Source: Journal of Inorganic Biochemistry - December 5, 2019 Category: Biochemistry Authors: Naso LG, Martínez Medina JJ, D'Alessandro F, Rey M, Rizzi A, Piro OE, Echeverría GA, Ferrer EG, Williams PAM Tags: J Inorg Biochem Source Type: research

DFT analysis, spectroscopic study and biological activity of a newly synthesized benzoylhydrazone binuclear Cu(II) complex.
Abstract The current work reports a joint experimental and theoretical study of a novel Cu(II) complex [CuL(μ-CH3COO)]2, based on 2-acetylpyridine-benzoylhydrazone ligand (HL). The two Cu(II) atoms are five-coordinated, consisting of three NNO-donor atoms from the hidrazone ligand connected by acetate bridges. In addition to the structural analysis, the complete characterization includes magnetic susceptibility, elemental analysis, FT-IR and UV-Vis. Two different approaches of Density Functional Theory (DFT) with localized basis set and plane waves were performed. The theoretical calculations were used to optim...
Source: Journal of Inorganic Biochemistry - November 29, 2019 Category: Biochemistry Authors: Santiago PHO, Tiago FS, Castro MS, Souza PEN, Martins JBL, Gatto CC Tags: J Inorg Biochem Source Type: research

Anti-leishmanial activity and cytotoxicity of a series of tris-aryl Sb(V) mandelate cyclometallate complexes.
Abstract A series of ten cyclometallates and two μ2-peroxo bridged tris-aryl Sb(V) complexes derived from R/S-mandelic acid (= R/S-ManH2) were synthesised and characterised. As confirmed by X-ray crystallography the complexes 1Sr/s, [Sb(o-tol)3(man)], 2Sr/s, [Sb(m-tol)3(man)], 4Sr/s, [Sb(o-PhOMe)3(man)], 5Sr/s, [Sb(Mes)3(man)] and 6Sr/s, [Sb(p-tert-BuPh)3(man)] are all cyclometallates. Complexes 3Sr/s, [(Sb(p-tol)3(manH)2O2], contain a bridging O22- anion in the solid-state but convert to the cyclometallates in DMSO solution with concomitant release of H2O2 and formation of complexes [Sb(p-tol)3(man)], 3Sr'/s'....
Source: Journal of Inorganic Biochemistry - November 23, 2019 Category: Biochemistry Authors: Duffin RN, Blair VL, Kedzierski L, Andrews PC Tags: J Inorg Biochem Source Type: research

Influence of synthesis methods on the internalization of fluorescent gold nanoparticles into glioblastoma stem-like cells.
D, Janiak C Abstract Glioblastoma (GBM) is an aggressive disease with currently no satisfying treatment option available. GBM cells with stem cell properties are thought to be responsible for the initiation and propagation of the disease, as well as main contributors to the emergence of therapy resistance. In this work, we developed a novel method to synthesize fluorescent gold nanoparticles as potential drug and gene delivery systems for GBM therapy, able to penetrate three-dimensional stem cell selected patient-derived GBM neurosphere systems in vitro. By using polyethylene imine (PEI) as a stabilizer and reduci...
Source: Journal of Inorganic Biochemistry - November 23, 2019 Category: Biochemistry Authors: Giesen B, Nickel AC, Garzón Manjón A, Vargas Toscano A, Scheu C, Kahlert UD, Janiak C Tags: J Inorg Biochem Source Type: research

Modified EDTA selectively recognized Cu2+ and its application in the disaggregation of β-amyloid-Cu (II)/Zn (II) aggregates.
Modified EDTA selectively recognized Cu2+ and its application in the disaggregation of β-amyloid-Cu (II)/Zn (II) aggregates. J Inorg Biochem. 2019 Nov 23;203:110929 Authors: Chang R, Chen X, Yu H, Tan G, Wen H, Huang J, Hao Z Abstract The accumulation of the β-amyloid (Aβ) aggregates induced by Cu2+/Zn2+ in conjunction with toxicity is closely related to Alzheimer's disease (AD). Herein, we intended to improve the efficiency and selectivity of traditional chelator ethylenediaminetetraacetic acid (EDTA) combined with a fluorescent group 4-aminosalicylic acid (4-ASA)to acquire a novel pot...
Source: Journal of Inorganic Biochemistry - November 23, 2019 Category: Biochemistry Authors: Chang R, Chen X, Yu H, Tan G, Wen H, Huang J, Hao Z Tags: J Inorg Biochem Source Type: research

Comparative study of the antitumoral activity of phosphine-thiosemicarbazone gold(I) complexes obtained by different methodologies.
aacute;lez-Noya AM, Pedrido R Abstract A series of phosphino-thiosemicarbazone gold(I) dinuclear complexes obtained by two different synthetic procedures have been prepared. All the compounds have been spectroscopically characterized including single crystal X ray diffraction analysis in some of cases. [Au2(HL1)Cl2] (1), [Au2(HL2)2]Cl2 (2) and [Au2(HL3)2]Cl2 (3) have been prepared by chemical synthesis using a gold(III) salt as precursor; while [Au2(L1)2] (4), [Au2(L2)2]∙2CH3CN (5) and [Au2(L3)2] (6) have been isolated from an electrochemical synthesis (HLn = 2-[2-(diphenylphosphanyl)-benzylidene]-N-R-thiose...
Source: Journal of Inorganic Biochemistry - November 22, 2019 Category: Biochemistry Authors: González-Barcia LM, Fernández-Fariña S, Rodríguez-Silva L, Bermejo MR, González-Noya AM, Pedrido R Tags: J Inorg Biochem Source Type: research