Interplay between autophagy and apoptosis in lead(II)-induced cytotoxicity of primary rat proximal tubular cells.
Abstract Autophagy and apoptosis are two different biological processes that determine cell fates. We previously reported that autophagy inhibition and apoptosis induction are involved in lead(II)-induced cytotoxicity in primary rat proximal tubular (rPT) cells, but the interplay between them remains to be elucidated. Firstly, data showed that lead(II)-induced elevation of LC3-II protein levels can be significantly modulated by 3-methyladenine or rapamycin; moreover, protein levels of Autophagy-related protein 5 (Atg5) and Beclin-1 were markedly up-regulated by lead(II) treatment, demonstrating that lead(II) could...
Source: Journal of Inorganic Biochemistry - February 24, 2018 Category: Biochemistry Authors: Chu BX, Fan RF, Lin SQ, Yang DB, Wang ZY, Wang L Tags: J Inorg Biochem Source Type: research

Bioactive ruthenium(II)-arene complexes containing modified 18 β-glycyrrhetinic acid ligands.
We report the synthesis, characterization and antiproliferative activity of two new Ru(II) arene complexes with imidazole (dichlorido complex 1) or bipyridyl (chlorido complex 2) ligands conjugated to 18β-glycyrrhetinic acid, an active triterpenoid metabolite of Glycyrrhiza glabra. In general, the conjugated ligands and complexes showed only moderate activity against HeLa (cervical), MCF-7 (breast) and A2780 (ovarian) cancer cells, although the activity of complex 2 in the former two cell lines approached that of the drug cisplatin. Complex 2 (in contrast to complex 1) also exhibited significant activity towards both ...
Source: Journal of Inorganic Biochemistry - February 21, 2018 Category: Biochemistry Authors: Kong Y, Chen F, Su Z, Qian Y, Wang FX, Wang X, Zhao J, Mao ZW, Liu HK Tags: J Inorg Biochem Source Type: research

Development of a novel carboplatin like cytoplasmic trackable near infrared fluorophore conjugate via strain-promoted azide alkyne cycloaddition.
Abstract The successful design and pre-clicked synthesis of a non-toxic and cytosol trackable carboplatin-like near infrared fluorophore conjugate via strain-promoted azide alkyne cycloaddition (SPAAC) is reported. Reaction of cis-[Pt(2-azidopropane-1,3-diamine)(cbdca)] (cbdca = cyclobutane-1,1-dicarboxylato) and a bicyclo[6.1.0]non-4-yne near-infrared (NIR) azadipyrromethene fluorophore gave the corresponding clicked Pt-Fluorophore conjugate. The X-ray crystal structure of cis-[Pt(2-azidopropane-1,3-diamine)(cbdca)] was determined featuring the azide on the bidentate 1,3-diaminopropane ligand. The Pt-fluoroph...
Source: Journal of Inorganic Biochemistry - February 21, 2018 Category: Biochemistry Authors: Kitteringham E, Wu D, Cheung S, Twamley B, O'Shea DF, Griffith DM Tags: J Inorg Biochem Source Type: research

Formation of Cys-heme cross-link in K42C myoglobin under reductive conditions with molecular oxygen.
This study provides valuable insights into the post-translational modification of heme proteins, and also suggests that the Cys-heme cross-link can be induced to form in vitro, making it useful for design of new heme proteins with a non-dissociable heme and improved functions. PMID: 29477977 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - February 19, 2018 Category: Biochemistry Authors: Cheng HM, Yuan H, Wang XJ, Xu JK, Gao SQ, Wen GB, Tan X, Lin YW Tags: J Inorg Biochem Source Type: research

Direct imaging of how lanthanides break the normal evolution of plants.
Abstract After rare earth elements [REE(III)] are anchored outside of the plasma membrane, REE(III) break plant evolution to initiate leaf cell endocytosis, which finally affects plant growth. However, the molecule for anchoring REE(III) in the acidic environment outside of the plasma membrane is not clear, which is crucial for exploring the mechanism of REE(III) breaking plant evolution. Here, lanthanum(III) [La(III)] and terbium(III) [Tb(III)] were respectively served as a representative of REE(III) without and with f electrons, and Arabidopsis was served as a representative of plants, cellular and molecular bas...
Source: Journal of Inorganic Biochemistry - February 10, 2018 Category: Biochemistry Authors: Yang Q, Wang L, He J, Yang Z, Huang X Tags: J Inorg Biochem Source Type: research

The effects of long duration chronic exposure to hexavalent chromium on single live cells interrogated by scanning electrochemical microscopy.
This study explores the effects of long duration chronic exposure to Cr (VI) on live human cells. Herein, scanning electrochemical microscopy (SECM) depth scan imaging was employed to monitor the membrane permeability of single live human bladder cancer (T24) cells following incubation with various Cr (VI) concentration stimuli. SECM was used to provide insights into the long duration effects on membrane homeostasis of individual cells exposed to constant levels of Cr (VI). Further investigation of total population viability was performed by MTT assay. Dependent on the exposure time, transition between three distinct trend...
Source: Journal of Inorganic Biochemistry - February 10, 2018 Category: Biochemistry Authors: Filice FP, Li MSM, Wong JM, Ding Z Tags: J Inorg Biochem Source Type: research

A family of manganese complexes containing heterocyclic-based ligands with cytotoxic properties.
We describe the synthesis of three new manganese (II) complexes containing the bidentate ligands 2-(1-methyl-3-pyrazolyl)pyridine (pypz-Me) and ethyl 2-(3-(pyridine-2-yl)-1H-pyrazol-1-yl)acetate (pypz-CH2COOEt), with formula [MnX2(pypz-Me)2] (X = Cl-1, CF3SO3-2) and [Mn(CF3SO3)2(pypz-CH2COOEt)2] 3. Complexes 1-3 have been characterized through analytical, spectroscopic and electrochemical techniques, as well as by monocrystal X-ray diffraction analysis. The complexes show a six-coordinated Mn(II) ion though different stereoisomers have been isolated for the three compounds. Complexes 1-3, together with the previously d...
Source: Journal of Inorganic Biochemistry - February 10, 2018 Category: Biochemistry Authors: Castro J, Manrique E, Bravo M, Vilanova M, Benito A, Fontrodona X, Rodríguez M, Romero I Tags: J Inorg Biochem Source Type: research

Reactions of ferric hemoglobin and myoglobin with hydrogen sulfide under physiological conditions.
Abstract Ferric hemoglobin (metHb) and myoglobin (metMb), present at low levels in vivo, have been recently found to oxidize hydrogen sulfide (H2S) in excess, thus potentially contributing to removal of toxic H2S in blood and heart, respectively. Here, we present a kinetic and thermodynamic study of the reaction of metHb and metMb with H2S under physiological conditions, i.e. at low H2S concentrations and with protein in excess of H2S. We show here that both proteins react with sub-stoichiometric H2S:heme ratios following two processes: a fast reversible binding of H2S to ferric heme that prevails at high H2S and ...
Source: Journal of Inorganic Biochemistry - February 10, 2018 Category: Biochemistry Authors: Jensen B, Fago A Tags: J Inorg Biochem Source Type: research

Evolution and molecular mechanism of PitAs in iron transport of Streptococcus species.
Abstract Iron is an essential element for almost all bacteria. The iron ATP-binding cassette (ABC) transporters located on the cell membrane affects bacterial virulence and infection. Although a variety of Fe3+-transporters have been found in bacteria, their evolutionary processes are rarely studied. Pneumococcal iron ABC transporter (PitA), a highly conserved Fe3+-transporter in most pathogenic bacteria, influences the capsule formation and virulence of bacteria. However, multiple sequence alignment revealed that PitA is expressed in four different variants in bacteria, and the structural complexity of these vari...
Source: Journal of Inorganic Biochemistry - February 10, 2018 Category: Biochemistry Authors: Cao K, Zhang J, Miao XY, Wei QX, Zhao XL, He QY, Sun X Tags: J Inorg Biochem Source Type: research

Synthesis, characterisation and DNA intercalation studies of regioisomers of ruthenium (II) polypyridyl complexes.
Abstract Regioisomers of the functional group of the main ligand (L) on a series of [Ru(phen)2L]2+and [Ru(bpy)2L]2+ complexes, where phen is 1,10 phenanthroline and bpy is 2,2'-bipyridine, were synthesised to investigate the interaction with deoxyribonucleic acid (DNA) as potential therapeutics. UV-Vis binding titrations, thermal denaturation and circular dichroism were used to evaluate their interaction with DNA. The conclusions indicated the significance of the auxiliary ligand; especially 1,10-phenanthroline has on the binding constants (Kb). The systematic variation of auxiliary ligand(phen or bpy), and polypy...
Source: Journal of Inorganic Biochemistry - February 9, 2018 Category: Biochemistry Authors: Perdisatt L, Moqadasi S, O'Neill L, Hessman G, Ghion A, Warraich MQM, Casey A, O'Connor C Tags: J Inorg Biochem Source Type: research

Thiocarbonyl-bound metallonitrosyl complexes with visible-light induced DNA cleavage and promising vasodilation activity.
s LGF Abstract Nitric oxide has been involved in many key biological processes such as vasodilation, platelet aggregation, apoptosis, memory function, and this has drawn attention to the development of exogenous NO donors. Metallonitrosyl complexes are an important class of these compounds. Here, two new ruthenium nitrosyl complexes containing a thiocarbonyl ligand, with the formula cis-[Ru(phen)2(L)(NO)](PF6)3 (phen = phenantroline, L = thiourea or thiobenzamide), were synthesized and characterized by electronic spectroscopy, FTIR, NMR, mass spectrometry and voltammetric techniques. Theoretical calculatio...
Source: Journal of Inorganic Biochemistry - February 9, 2018 Category: Biochemistry Authors: Silva CDS, Paz IA, Abreu FD, de Sousa AP, Veríssimo CP, Nascimento NRF, Paulo TF, Zampieri D, Eberlin MN, Gondim ACS, Andrade LC, Carvalho IMM, Sousa EHS, Lopes LGF Tags: J Inorg Biochem Source Type: research

Water soluble glucose derivative of thiocarbohydrazone acts as ionophore with cytotoxic effects on tumor cells.
Abstract A novel water-soluble ionophore based on the thiocarbohydrazone moiety conjugated with glucose (GluTch) was synthesized through a simple two-step procedure. Structural elucidation was carried out in water solution by means of various spectroscopic techniques (NMR, UV-Vis, and CD), electrospray ionization mass spectrometry and density functional theory calculations. The flexible nature of the thiocarbohydrazone moiety of the new glycoderivative compound induced both different coordination motifs and stoichiometry towards copper and zinc. Cytotoxicity assays of the ligands on the human normal keratinocyte N...
Source: Journal of Inorganic Biochemistry - February 9, 2018 Category: Biochemistry Authors: Bonaccorso C, Grasso G, Musso N, Barresi V, Condorelli DF, La Mendola D, Rizzarelli E Tags: J Inorg Biochem Source Type: research

Study of manganese binding to the ferroxidase centre of human H-type ferritin.
Abstract Ferritins are ubiquitous and conserved proteins endowed with enzymatic ferroxidase activity, that oxidize Fe(II) ions at the dimetal ferroxidase centre to form a mineralized Fe(III) oxide core deposited within the apo-protein shell. Herein, the in vitro formation of a heterodimetal cofactor constituted by Fe and Mn ions has been investigated in human H ferritin (hHFt). Namely, Mn and Fe binding at the hHFt ferroxidase centre and its effects on Fe(II) oxidation have been investigated by UV-Vis ferroxidation kinetics, fluorimetric titrations, multifrequency EPR, and preliminary Mössbauer spectroscopy. ...
Source: Journal of Inorganic Biochemistry - February 9, 2018 Category: Biochemistry Authors: Ardini M, Howes BD, Fiorillo A, Falvo E, Sottini S, Rovai D, Lantieri M, Ilari A, Gatteschi D, Spina G, Chiancone E, Stefanini S, Fittipaldi M Tags: J Inorg Biochem Source Type: research

The Cu(II) affinity of the N-terminus of human copper transporter CTR1: Comparison of human and mouse sequences.
Abstract Copper Transporter 1 (CTR1) is a homotrimeric membrane protein providing the main route of copper transport into eukaryotic cells from the extracellular milieu. Its N-terminal extracellular domain, rich in His and Met residues, is considered responsible for directing copper into the transmembrane channel. Most of vertebrate CTR1 proteins contain the His residue in position three from N-terminus, creating a well-known Amino Terminal Cu(II)- and Ni(II)-Binding (ATCUN) site. CTR1 from humans, primates and many other species contains the Met-Asp-His (MDH) sequence, while some rodents including mouse have the ...
Source: Journal of Inorganic Biochemistry - February 2, 2018 Category: Biochemistry Authors: Bossak K, Drew SC, Stefaniak E, Płonka D, Bonna A, Bal W Tags: J Inorg Biochem Source Type: research

Tuning the MnII2/MnIII2 redox cycle of a phenoxo-bridged diMn catalase mimic with terminal carboxylate donors.
ignorella S Abstract A new phenoxo-bridged diMnIII complex, Na[Mn2L(OH)2(H2O)2]·5H2O (1), obtained with the ligand L5- = 5‑methyl‑2‑hydroxo‑1,3‑xylene‑α,α‑diamine‑N,N,N',N'‑tetraacetato, has been prepared and characterized. Mass spectrometry, conductivity, UV-visible, EPR and 1H NMR spectroscopic studies showed that the complex exists in solution as a monoanionic diMnIII complex. Complex 1 catalyzes H2O2 disproportionation with second-order rate constant kcat = 305(9) M-1 min-1 and without a time-lag phase. Based on spectroscopic results, the catalase activity of co...
Source: Journal of Inorganic Biochemistry - February 2, 2018 Category: Biochemistry Authors: Solís V, Palopoli C, Daier V, Rivière E, Collin F, Moreno DM, Hureau C, Signorella S Tags: J Inorg Biochem Source Type: research

Carboxyl groups of citric acid in the process of complex formation with bivalent and trivalent metal ions in biological systems.
Abstract Binary complexes of citric acid (H3L - protonated form, H2L and HL - partly protonated forms, L - fully deprotonated) with d- and f-electron metal ions were investigated. The studies have been performed in aqueous solution using the potentiometric method with computer analysis of the data, electron paramagnetic resonance, infrared, visible as well as luminescence spectroscopies. The overall stability constants of the complexes were determined. Analysis of the equilibrium constants of the reactions and spectroscopic data has allowed determination of the type of coordination and effectiveness of the carboxy...
Source: Journal of Inorganic Biochemistry - February 2, 2018 Category: Biochemistry Authors: Zabiszak M, Nowak M, Taras-Goslinska K, Kaczmarek MT, Hnatejko Z, Jastrzab R Tags: J Inorg Biochem Source Type: research

RTHLVFFARK-NH2: A potent and selective modulator on Cu2+-mediated amyloid- β protein aggregation and cytotoxicity.
RTHLVFFARK-NH2: A potent and selective modulator on Cu2+-mediated amyloid-β protein aggregation and cytotoxicity. J Inorg Biochem. 2018 Feb 02;181:56-64 Authors: Meng J, Zhang H, Dong X, Liu F, Sun Y Abstract Dysfunctional accumulation of amyloid-β (Aβ) protein stimulated by Cu2+ is considered as a key process in the pathogenesis of Alzheimer's disease (AD). Thus, bifunctional substances capable of chelating Cu2+ and inhibiting Aβ aggregation are promising therapeutic agents against AD. Herein, a novel bifunctional decapeptide RTHLVFFARK-NH2 (RK10) was developed by integrating a me...
Source: Journal of Inorganic Biochemistry - February 2, 2018 Category: Biochemistry Authors: Meng J, Zhang H, Dong X, Liu F, Sun Y Tags: J Inorg Biochem Source Type: research

An investigation of the pH dependence of copper-substituted anthrax lethal factor and its mechanistic implications.
Abstract Anthrax lethal factor (LF) is a zinc-dependent endopeptidase involved in the cleavage of proteins critical to the maintenance of host signaling pathways during anthrax infections. Although zinc is typically regarded as the native metal ion in vivo, LF is highly tolerant to metal substitution, with its replacement by copper yielding an enzyme (CuLF) 4.5-fold more active than the native zinc protein (at pH 7). The current study demonstrates that by careful choice of the buffer, ionic strength, pH and substrate, the activity ratio of CuLF and native LF can be increased to>40-fold. Using a fluorogenic LF...
Source: Journal of Inorganic Biochemistry - February 1, 2018 Category: Biochemistry Authors: Young CJ, Richard K, Beruar A, Lo SY, Siemann S Tags: J Inorg Biochem Source Type: research

Synthesis, characterization and cytotoxic activity of novel copper(II) complexes with aroylhydrazone derivatives of 2-Oxo-1,2-dihydrobenzo[h]quinoline-3-carbaldehyde.
Abstract Three new 2-oxo-1,2-dihydrobenzo[h]quinoline-3-carbaldehyde terminal substituted aroylhydrazone ligands (2-Oxo-1,2-dihydrobenzo[h]quinoline-3-carbaldehyde(2'-hydroxybenzoyl)hydrazine, H2L1, 1, 2-Oxo-1,2-dihydrobenzo[h]quinoline-3-carbaldehyde(2'-hydroxybenzoyl)hydrazine, H2L2, 2, 2-Oxo-1,2-dihydrobenzo[h]quinoline-3-carbaldehyde(2'-hydroxybenzoyl)hydrazine, H2L3, 3) and the corresponding novel copper(II) complexes [Cu(L)(CH3OH)(NO3)](L = HL1 (4), HL2 (5), HL3 (6-6+), have been synthesized to compare their coordination behaviour and biological activity with respect to the presence of an OH group in dif...
Source: Journal of Inorganic Biochemistry - February 1, 2018 Category: Biochemistry Authors: Ramachandran E, Gandin V, Bertani R, Sgarbossa P, Natarajan K, Bhuvanesh NSP, Venzo A, Zoleo A, Glisenti A, Dolmella A, Albinati A, Marzano C Tags: J Inorg Biochem Source Type: research

Europium(III)-doped yttrium vanadate nanoparticles reduce the toxicity of cisplatin.
es DC Abstract The aim of this study was to evaluate the antitumor efficiency of chemotherapy with cisplatin alone and incorporated into europium(III)-doped yttrium vanadate nanoparticles functionalized with 3‑chloropropyltrimethoxysilane with folic acid and without folic acid in a syngeneic mouse melanoma model. Histopathological, biochemical and genotoxic analyses of treated animals were performed to assess the toxicity of treatments. The treatment of the animals with cisplatin alone and the nanoparticles functionalized with cisplatin at a dose of 5 mg/kg b.w. for 5 days reduced tumor weight about 86% and ...
Source: Journal of Inorganic Biochemistry - February 1, 2018 Category: Biochemistry Authors: Ferreira NH, Furtado RA, Ribeiro AB, de Oliveira PF, Ozelin SD, de Souza LDR, Neto FR, Miura BA, Magalhães GM, Nassar EJ, Tavares DC Tags: J Inorg Biochem Source Type: research

Silver complex of salicylic acid and its hydrogel-cream in wound healing chemotherapy.
Abstract The known metallotherapeutic [Ag(salH)]2 (AGSAL-1) of salicylic acid (salH2), was used for the development of new efficient silver based material for wounds healing. AGSAL-1 was characterized by spectroscopic techniques and X-ray crystallography. The wound healing epithelialization of AGSAL-1 was investigated by the means of scratch assay against immortalized human keratinocytes (HaCaT) cells. The anti-inflammatory activity of AGSAL-1 was evaluated by monitoring the catalytic peroxidation of linoleic acid to hydroperoxylinoleic acid by the enzyme lipoxygenase (LOX). The antibacterial activity of AGSAL-1 w...
Source: Journal of Inorganic Biochemistry - February 1, 2018 Category: Biochemistry Authors: Stathopoulou MK, Banti CN, Kourkoumelis N, Hatzidimitriou AG, Kalampounias AG, Hadjikakou SK Tags: J Inorg Biochem Source Type: research

Dynamics of dehaloperoxidase-hemoglobin A derived from NMR relaxation spectroscopy and molecular dynamics simulation.
Abstract Dehaloperoxidase-hemoglobin is the first hemoglobin identified with biologically-relevant oxidative functions, which include peroxidase, peroxygenase and oxidase activities. Herein we report a study of the protein backbone dynamics of DHP using heteronuclear NMR relaxation methods and molecular dynamics (MD) simulations to address the role of protein dynamics in switching from one function to another. The results show that DHP's backbone helical regions and turns have average order parameters of S2 = 0.87 ± 0.03 and S2 = 0.76 ± 0.08, respectively. Furthermore, DHP is primaril...
Source: Journal of Inorganic Biochemistry - January 12, 2018 Category: Biochemistry Authors: Zhao J, Xue M, Gudanis D, Gracz H, Findenegg GH, Gdaniec Z, Franzen S Tags: J Inorg Biochem Source Type: research

Ruthenium dendrimers as carriers for anticancer siRNA.
This article is devoted to study interactions between new carbosilane-based metallodendrimers containing ruthenium and anti-cancer small interfering RNA (siRNA). Formation of complexes between anti-cancer siRNAs and Ru-based carbosilane dendrimers was evaluated by transmission electron microscopy, circular dichroism and fluorescence. The zeta-potential and the size of dendriplexes were determined by dynamic light scattering. The internalization of dendriplexes were estimated using HL-60 cells. Results show that ruthenium dendrimers associated with anticancer siRNA have the ability to deliver siRNA as non-viral vectors into...
Source: Journal of Inorganic Biochemistry - January 12, 2018 Category: Biochemistry Authors: Michlewska S, Ionov M, Maroto-Díaz M, Szwed A, Ihnatsyeu-Kachan A, Loznikova S, Shcharbin D, Maly M, Ramirez RG, de la Mata FJ, Bryszewska M Tags: J Inorg Biochem Source Type: research

Potential drug targets in the Mycobacterium tuberculosis cytochrome P450 system.
Abstract The Mycobacterium tuberculosis genome encodes twenty cytochrome P450 enzymes, most or all of which appear to have specific physiological functions rather than being devoted to the removal of xenobiotics. However, in many cases their specific functions remain obscure. Considerable spectroscopic, biophysical, crystallographic, and catalytic information is available on nine of these cytochrome P450 enzymes, although gaps exist in our knowledge of even these enzymes. The available evidence indicates that at least three of the better-characterized enzymes are promising targets for antituberculosis drug discove...
Source: Journal of Inorganic Biochemistry - January 12, 2018 Category: Biochemistry Authors: Ortiz de Montellano PR Tags: J Inorg Biochem Source Type: research

CuI and CuII complexes with phosphine derivatives of fluoroquinolone antibiotics - A comparative study on the cytotoxic mode of action.
Abstract In this paper, we present a comparative study on the cytotoxic mode of action of copper(I) and copper(II) complexes with phosphine derivatives of fluoroquinolone antibiotics (ciprofloxacin HCp and norfloxacin HNr). The in vitro cytotoxic activity of four new compounds was tested against two selected cancer cell lines. All complexes exhibited much better cytotoxicity against both cell lines than unmodified fluoroquinolone antibiotics, their phosphines (PCp, PNr), chalcogenide derivatives (oxides: OPCp, OPNr; sulfides: SPCp, SPNr and selenides: SePCp, SePNr) and previously described by us complexes with pho...
Source: Journal of Inorganic Biochemistry - January 9, 2018 Category: Biochemistry Authors: Bykowska A, Komarnicka UK, Jeżowska-Bojczuk M, Kyzioł A Tags: J Inorg Biochem Source Type: research

Cytotoxic and anticancer properties of new ruthenium polypyridyl complexes with different lipophilicities.
Abstract Three ruthenium complexes containing a bidentate piq ligand, [(piq)Ru(bpy)2]2+ (1), [(piq)Ru(phen)2]2+ (2), and [(piq)Ru(DIP)2]2+ (3) (piq = phenylisoquinolinate, bpy = 2,2'-bipyridine, phen = 1,10-phenanthroline, DIP = 4,7-diphenyl-1,10-phenanthroline), were prepared. The DNA binding properties of complexes 1-3 to double-stranded DNA were studied. The binding of 1-3 to calf-thymus DNA (ct-DNA) yielded lower emission intensities than those observed with the corresponding Ru complexes alone. To explore potential interactions of complexes 1-3 with lipid-rich organs in live cells, the emissio...
Source: Journal of Inorganic Biochemistry - January 9, 2018 Category: Biochemistry Authors: Florence Tikum A, Jeon YJ, Lee JH, Park MH, Bae IY, Kim SH, Lee HJ, Kim J Tags: J Inorg Biochem Source Type: research

Disulfide-masked iron prochelators: Effects on cell death, proliferation, and hemoglobin production.
This study provides new information on the intracellular effects of disulfide-based prochelators and indicates aroylhydrazone (AH1-S)2 as a promising prototype of a new class of antiproliferative prochelator systems. PMID: 29324291 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - January 4, 2018 Category: Biochemistry Authors: Akam EA, Utterback RD, Marcero JR, Dailey HA, Tomat E Tags: J Inorg Biochem Source Type: research

The role of Ala134 in controlling substrate binding and reactivity in ascorbate peroxidase.
Abstract Ascorbate peroxidase (APX) is a class I heme peroxidase. It has two sites for binding of substrates. One is close to the γ-heme edge and is used for oxidation of ascorbate; the other is at the δ-heme edge and is used for binding of aromatic substrates [Gumiero et al., (2010) Arch. Biochem. Biophys. 500, 13-20]. In this work, we have examined the structural factors that control binding at the δ-heme edge by replacement of Ala134 in APX with a proline residue that is more commonly found in other class II and III peroxidases. Kinetic data indicate that replacement of Ala134 by proline has o...
Source: Journal of Inorganic Biochemistry - December 29, 2017 Category: Biochemistry Authors: Turner DD, Lad L, Kwon H, Basran J, Carr KH, Moody PCE, Raven EL Tags: J Inorg Biochem Source Type: research

Imaging of a clickable anticancer iridium catalyst.
Abstract Iridium-based anticancer reagents are receiving increasing attention for their high cytotoxicity. Herein, by activating CH bonds in the well-known antioxidant α-phenyl-N-tert-butylnitrone (PBN), we synthesized and characterized a series of new iridium complexes. Complex 1-AMP exhibited the best antiproliferation activity towards human ovarian cancer A2780 cells. The azide group in complex 1-AMP underwent the Cu(I)-catalysed azide-alkyne cycloaddition (CuAAC) reaction and the resulting fluorescent imaging in cells suggested it mainly accumulated in mitochondria. In comparison, to eliminate cytotoxici...
Source: Journal of Inorganic Biochemistry - December 29, 2017 Category: Biochemistry Authors: Wang X, Zhu M, Gao F, Wei W, Qian Y, Liu HK, Zhao J Tags: J Inorg Biochem Source Type: research

Incorporation of the chemotherapy medication cisplatin into polyamide membrane.
ia IA Abstract The search for more efficient and less aggressive cancer treatment methods has intensified over the last decades and has involved many scientific areas. To provide skin cancer patients with better quality of life, this work aims to incorporate chemotherapy into polyamide membranes, functionalized by the sol-gel methodology, for controlled drug release at the target tissue. A 200-micrometer-thick flexible polyamide membrane prepared by Additive Manufacture was activated and functionalized with the alkoxide 3-chloropropyltriethoxysilane, which was followed by incorporation of the antitumor agent cispl...
Source: Journal of Inorganic Biochemistry - December 24, 2017 Category: Biochemistry Authors: de Souza ÉA, Rocha LA, de Faria EH, Ciuffi KJ, Nassar EJ, Silva JVL, Oliveira MF, Maia IA Tags: J Inorg Biochem Source Type: research

Amphoteric phosphorous(V)-phthalocyanines as proton-driven switchable fluorescers toward deep-tissue bio-imaging.
Abstract Spectral (optical absorption and emission) properties of three amphoteric phosphorous(V)-phthalocyanine derivatives, [P(Pc)(O)OH], where Pc=tetra(tert-butyl)phthalocyaninate (tbpc), tetrakis(2',6'-dimethylphenoxy)phthalocyaninate (tppc), and octakis(4'-tert-butylphenoxy)phthalocyaninate (obppc), have been investigated in ethanolic solutions. Spectral changes upon protonation/deprotonation (the reaction sites have been determined to be their axial ligands by magnetic circular dichroism study) are drastic and rapid. All the initial ([P(Pc)(O)OH]), protonated ([P(Pc)(OH)2]+), and deprotonated ([P(Pc)(O)2]-) ...
Source: Journal of Inorganic Biochemistry - December 23, 2017 Category: Biochemistry Authors: Isago H, Fujita H, Sugimori T Tags: J Inorg Biochem Source Type: research

Delivery of [Ru( η6-p-cymene)Cl2{Ph2P(CH2)3SPh-κP}] using unfunctionalized and mercapto functionalized SBA-15 mesoporous silica: Preparation, characterization and in vitro study.
Delivery of [Ru(η6-p-cymene)Cl2{Ph2P(CH2)3SPh-κP}] using unfunctionalized and mercapto functionalized SBA-15 mesoporous silica: Preparation, characterization and in vitro study. J Inorg Biochem. 2017 Dec 22;180:155-162 Authors: Edeler D, Arlt S, Petković V, Ludwig G, Drača D, Maksimović-Ivanić D, Mijatović S, Kaluđerović GN Abstract SBA-15 (Santa Barbara Amorphous 15) mesoporous silica and its functionalized form (with 3-mercaptopropyltriethoxysilane) SBA-15~SH were used as carriers for [Ru(η6-p-cymene)Cl2{Ph2P(CH2)3SPh-κP}] complex, denoted as [Ru]. Prepared mesoporous sili...
Source: Journal of Inorganic Biochemistry - December 22, 2017 Category: Biochemistry Authors: Edeler D, Arlt S, Petković V, Ludwig G, Drača D, Maksimović-Ivanić D, Mijatović S, Kaluđerović GN Tags: J Inorg Biochem Source Type: research

Structural characterization of the nitrogenase molybdenum-iron protein with the substrate acetylene trapped near the active site.
Abstract The biological reduction of dinitrogen (N2) to ammonia is catalyzed by the complex metalloenzyme nitrogenase. Structures of the nitrogenase component proteins, Iron (Fe) protein and Molybdenum‑iron (MoFe) protein, and the stabilized complexes these component proteins, have been determined, providing a foundation for a number of fundamental aspects of the complicated catalytic mechanism. The reduction of dinitrogen to ammonia is a complex process that involves the binding of N2 followed by reduction with multiple electrons and protons. Electron transfer into nitrogenase is typically constrained to the un...
Source: Journal of Inorganic Biochemistry - December 13, 2017 Category: Biochemistry Authors: Keable SM, Vertemara J, Zadvornyy OA, Eilers BJ, Danyal K, Rasmussen AJ, De Gioia L, Zampella G, Seefeldt LC, Peters JW Tags: J Inorg Biochem Source Type: research

Physicochemical, antioxidant, DNA cleaving properties and antimicrobial activity of fisetin-copper chelates.
Abstract Fisetin (3,3',4',7-tetrahydroxyflavone) metal chelates are of interest as this plant polyphenol has revealed broad prospects for its use as natural medicine in the treatment of various diseases. Metal interactions may change or enhance fisetin biological properties so understanding fisetin metal chelation is important for its application not only in medicine but also as a food additive in nutritional supplements. This work was aimed to determine and characterize copper complexes formed in different pH range at applying various metal/ligand ratios. Fisetin and Cu(II)-fisetin complexes were characterized by...
Source: Journal of Inorganic Biochemistry - December 11, 2017 Category: Biochemistry Authors: Łodyga-Chruscińska E, Pilo M, Zucca A, Garribba E, Klewicka E, Rowińska-Żyrek M, Symonowicz M, Chrusciński L, Cheshchevik VT Tags: J Inorg Biochem Source Type: research

Revisiting the thiosemicarbazonecopper(II) reaction with glutathione. Activity against colorectal carcinoma cell lines.
z P, Palacios Ò, Samper KG, Rojo T Abstract Thiosemicarbazones (TSCs), and their copper derivatives, have been extensively studied mainly due to the potential applications as antitumor compounds. A part of the biological activity of the TSC-CuII complexes rests on their reactivity against cell reductants, as glutathione (GSH). The present paper describes the structure of the [Cu(PTSC)(ONO2)]n compound (1) (HPTSC=pyridine-2-carbaldehyde thiosemicarbazone) and its spectroscopic and magnetic properties. ESI studies performed on the reaction of GSH with 1 and the analogous [{Cu(PTSC*)(ONO2)}2] derivative (2, HP...
Source: Journal of Inorganic Biochemistry - December 8, 2017 Category: Biochemistry Authors: García-Tojal J, Gil-García R, Fouz VI, Madariaga G, Lezama L, Galletero MS, Borrás J, Nollmann FI, García-Girón C, Alcaraz R, Cavia-Saiz M, Muñiz P, Palacios Ò, Samper KG, Rojo T Tags: J Inorg Biochem Source Type: research

Synthesis and evaluation of the antibiotic and adjuvant antibiotic potential of organotin(IV) derivatives.
-Junior FJB Abstract A series of organotin(IV) derivatives was investigated in vitro for their antibiotic and adjuvant antibiotic properties (efflux pump inhibitors) against Staphylococcus aureus strains that overexpress efflux pump proteins for norfloxacin (SA-1199B), erythromycin (RN-4220) and tetracycline (IS-58). Most organotin(IV) compounds showed significant antibacterial activity with small Minimum Inhibitory Concentration (MIC) values, some of which were close to 1.0μg/mL (3.1μM), but this feature was also associated with substantial cytotoxicity. Nevertheless, the cytotoxicity of these organotin(IV)...
Source: Journal of Inorganic Biochemistry - December 8, 2017 Category: Biochemistry Authors: Barbosa ASL, Guedes JS, da Silva DR, Meneghetti SMP, Meneghetti MR, da Silva AE, de Araujo MV, Alexandre-Moreira MS, de Aquino TM, de Siqueira Junior JP, de Araújo RSA, da Cruz RMD, Mendonça-Junior FJB Tags: J Inorg Biochem Source Type: research

Simple and mixed complexes of copper(II) with 8-hydroxyquinoline derivatives and amino acids: Characterization in solution and potential biological implications.
ve; G Abstract Copper(II) complexes with 8-hydroxyquinoline (8-HQ) and two 8-HQ derivatives, namely clioquinol (CQ) and 5,7-dichloro-2-[(dimethylamino)methyl]quinolin-8-ol (PBT2), were investigated in organic and, where feasible, in aqueous solutions. This class of compounds is of particular interest in neurological disorders since they may act as metal-protein attenuating compounds and may help redistributing metal ions and restoring intracellular metal reserves, which are often perturbed in neurological patients. Several techniques, like potentiometry, UV-Vis absorption, electron paramagnetic resonance (EPR), cy...
Source: Journal of Inorganic Biochemistry - December 8, 2017 Category: Biochemistry Authors: Sgarlata C, Arena G, Bonomo RP, Giuffrida A, Tabbì G Tags: J Inorg Biochem Source Type: research

Engineering a self-sufficient Mycobacterium tuberculosis CYP130 by gene fusion with the reductase-domain of CYP102A1 from Bacillus megaterium.
Abstract CYP130 belongs to the subset of cytochrome P450s from Mycobacterium tuberculosis (Mtb) that have been structurally characterized. Despite several efforts for its functional characterization, CYP130 is still considered an orphan enzyme for which no endogenous or exogenous substrate has been identified. In addition, functional redox-partners for CYP130 have not been clearly established yet, hampering the elucidation of its physiological role. In the present study, a catalytically active fusion protein involving CYP130 and the NADPH reductase-domain of CYP102A1 from Bacillus megaterium was created. By screen...
Source: Journal of Inorganic Biochemistry - December 7, 2017 Category: Biochemistry Authors: Ortega Ugalde S, Luirink RA, Geerke DP, Vermeulen NPE, Bitter W, Commandeur JNM Tags: J Inorg Biochem Source Type: research

Copper(II) complexes based on tripodal pyrazolyl amines: Synthesis, structure, magnetic properties and anticancer activity.
;vníček Z Abstract The Cu(II) complexes [Cu(bpdmpz)Cl]ClO4 (1), [Cu(bdmpzp)Cl]ClO4 (2-ClO4), [Cu(bdmpzp)Cl]PF6 (2-PF6) and [Cu(tdmpza)Cl]ClO4 (3), bpdmpzp=[bis[((2-pyridylmethyl)-di(3,5-dimethyl-1H-pyrazolyl)methyl)]amine, bdmpzp=[bis((di(3,5-dimethyl-1H-pyrazolyl)methyl)-(2-pyridylmethyl)]amine and tdmpza=tris[di(3,5-dimethyl-1H-pyrazolyl)-methyl)]amine were synthesized and characterized by elemental analysis, magnetic and conductivity measurements, electrospray-ionization mass spectrometry, infrared and electronic spectroscopy, and X-ray crystallography. The magnetic properties of the complexes, measured...
Source: Journal of Inorganic Biochemistry - December 5, 2017 Category: Biochemistry Authors: Massoud SS, Louka FR, Ducharme GT, Fischer RC, Mautner FA, Vančo J, Herchel R, Dvořák Z, Trávníček Z Tags: J Inorg Biochem Source Type: research

Modulation effect of double strand DNA on the self-assembly of N-terminal domain of Euplotes octocarinatus centrin.
Abstract Centrin is a member of the EF-hand super family of calcium-binding proteins, which can behave as a part of damage detector initiated the initiation of nucleotide excision repair (NER). Its self-assembly plays a causative role in fiber contraction associated with the cell division cycle and ciliogenesis. To explore the possible role of DNA in the process of centrin self-assembly, the aggregation properties of N-terminal domain of Euplotes octocarinatus centrin (N-EoCen) in the presence of DNA with or without metal ions are investigated. It is verified that metal ions, such as Ca2+ and Tb3+, can bind to N-E...
Source: Journal of Inorganic Biochemistry - December 5, 2017 Category: Biochemistry Authors: Zhang W, Shi E, Zhao Y, Yang B Tags: J Inorg Biochem Source Type: research

Theranostic liposomes as a bimodal carrier for magnetic resonance imaging contrast agent and photosensitizer.
Abstract The present study is focused on the development of liposomes bearing gadolinium chelate (GdLip) providing two functionalities for magnetic resonance imaging (MRI) and photodynamic therapy of cancer. A lipid derivative of gadolinium(III) diethylenetriamine pentaacetic acid salt (GdDTPA1) was inserted in the liposomal membrane and served as MRI contrast agent whereas a zinc phthalocyanine (ZnPc) was used as a model photosensitizer. In addition to conventional liposomes, pegylated lipids were used for the preparation of "stealth" liposomes. The characterization of different GdLip formulations invol...
Source: Journal of Inorganic Biochemistry - December 2, 2017 Category: Biochemistry Authors: Skupin-Mrugalska P, Sobotta L, Warowicka A, Wereszczynska B, Zalewski T, Gierlich P, Jarek M, Nowaczyk G, Kempka M, Gapinski J, Jurga S, Mielcarek J Tags: J Inorg Biochem Source Type: research

The in vitro renal cell toxicity of some unconventional anticancer phenanthroline-based platinum(II) complexes.
Abstract The cytotoxicity of platinum(II) complexes coordinated to a chiral diamine, 1S,2S-diaminocyclohexane or 1R,2R-diaminocyclohexane and 1,10-phenanthroline or 3,4,7,8-tetramethyl-1,10-phenanthroline has been investigated in the renal proximal tubule HK-2 cell line. All platinum(II) complexes exhibited lower cytotoxicity in HK-2 cells (IC50 1.7-25μM) than in A2780 ovarian cancer cells or cisplatin-resistant A2780cisR cells (IC50 0.2-2.1μM) (at 48h). PHENSS ([Pt(1,10-phenanthroline)(1S,2S-dach)]2+) induced apoptosis and necrosis in ovarian cancer cells at concentrations that are relatively cytostatic to ...
Source: Journal of Inorganic Biochemistry - November 30, 2017 Category: Biochemistry Authors: Ng NS, Wu MJ, Myers SJ, Aldrich-Wright JR Tags: J Inorg Biochem Source Type: research

Photolabile ruthenium complexes to cage and release a highly cytotoxic anticancer agent.
Abstract CHS-828 (N-(6-(4-chlorophenoxy)hexyl)-N'-cyano-N″-4-pyridyl guanidine) is an anticancer agent with low bioavailability and high systemic toxicity. Here we present an approach to improve the therapeutic profile of the drug using photolabile ruthenium complexes to generate light-activated prodrugs of CHS-828. Both prodrug complexes are stable in the dark but release CHS-828 when irradiated with visible light. The complexes are water-soluble and accumulate in tumour cells in very high concentrations, predominantly in the mitochondria. Both prodrug complexes are significantly less cyototoxic than free C...
Source: Journal of Inorganic Biochemistry - November 22, 2017 Category: Biochemistry Authors: Wei J, Renfrew AK Tags: J Inorg Biochem Source Type: research

Hydroxypyridinone-benzofuran hybrids with potential protective roles for Alzheimer ´s disease therapy.
Hydroxypyridinone-benzofuran hybrids with potential protective roles for Alzheimer´s disease therapy. J Inorg Biochem. 2017 Nov 21;179:82-96 Authors: Hiremathad A, Chand K, Tolayan L, Rajeshwari, Keri RS, Esteves AR, Cardoso SM, Chaves S, Santos MA Abstract A series of (3-hydroxy-4-pyridinone)-benzofuran hybrids have been developed and studied as potential multitargeting drugs for Alzheimer's disease (AD). Their design envisaged mainly to mimic the donepezil drug, a marketed inhibitor of acetylcholinesterase (AChE), and to endow the conjugate molecules with extra-properties such as metal chelati...
Source: Journal of Inorganic Biochemistry - November 21, 2017 Category: Biochemistry Authors: Hiremathad A, Chand K, Tolayan L, Rajeshwari, Keri RS, Esteves AR, Cardoso SM, Chaves S, Santos MA Tags: J Inorg Biochem Source Type: research

Neuronal death/apoptosis induced by intracellular zinc deficiency associated with changes in amino-acid neurotransmitters and glutamate receptor subtypes.
Abstract In the present study, a model of zinc deficiency was developed by exposing primary neurons to an N,N,N',N'-Tetrakis (2-pyridylmethyl) ethylenediamine (TPEN)-containing medium. The cell survival rate, apoptosis rate, intracellular and extracellular concentrations of 4 amino acids, and the expression of 2 glutamate receptor subtypes α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor (GluR2)and N-methyl-d-aspartate receptor subtype 2B (NR2B) were evaluated in zinc-deficient cells. The results revealed that zinc deficiency led to a decrease in cell viability and an increase in the apoptosis rate....
Source: Journal of Inorganic Biochemistry - November 21, 2017 Category: Biochemistry Authors: Tian K, Wang YX, Li LX, Liu YQ Tags: J Inorg Biochem Source Type: research

Effects of polar substituents on the biological activity of thiosemicarbazone metal complexes.
Abstract In this paper, citronellal, vanillin and pyridoxal thiosemicarbazones were modified with polar substituents, namely ethylmorpholine and glucose, to increase their polarity and compare the effects of these moieties on their biological activity. Altogether, nine ligands were synthesized and for each of them also their copper(II) and nickel(II) complexes were prepared and used for the biological tests. Eventually, assays on proliferation inhibition were conducted using leukemic cell line U937, already used as a model for previous citronellal thiosemicarbazone tests. Biological tests were also performed on so...
Source: Journal of Inorganic Biochemistry - November 21, 2017 Category: Biochemistry Authors: Bisceglie F, Tavone M, Mussi F, Azzoni S, Montalbano S, Franzoni S, Tarasconi P, Buschini A, Pelosi G Tags: J Inorg Biochem Source Type: research

Synthesis and mechanistic investigation of iron(II) complexes of isoniazid and derivatives as a redox-mediated activation strategy for anti-tuberculosis therapy.
te;nisson V Abstract The emergence of multidrug-resistant strains of Mycobacterium tuberculosis (MTB) represents a major threat to global health. Isoniazid (INH) is a prodrug used in the first-line treatment of tuberculosis. It undergoes oxidation by a catalase-peroxidase KatG, leading to generation of an isonicotinoyl radical that reacts with NAD(H) forming the INH-NADH adduct as the active metabolite. A redox-mediated activation of isoniazid using an iron metal complex was previously proposed as a strategy to overcome isoniazid resistance due to KatG mutations. Here, we have prepared a series of iron metal compl...
Source: Journal of Inorganic Biochemistry - November 21, 2017 Category: Biochemistry Authors: Laborde J, Deraeve C, de Mesquita Vieira FG, Sournia-Saquet A, Rechignat L, Villela AD, Abbadi BL, Macchi FS, Pissinati K, Bizarro CV, Machado P, Basso LA, Pratviel G, de França Lopes LG, Sousa EHS, Bernardes-Génisson V Tags: J Inorg Biochem Source Type: research

HOCl and the control of oncogenesis.
Abstract Sustained generation of extracellular superoxide anions by membrane-associated NADPH oxidase-1 is a hallmark of malignant transformation. The resulting H2O2 drives the proliferation of malignant cells and is converted to HOCl by a Dual oxidase-related peroxidase domain that acts analogously to myeloperoxidase. Whereas H2O2 induces apoptosis nonselectively in nontransformed and transformed cells, HOCl selectively affects malignant cells, as the interaction between HOCl and extracellular superoxide anions allows for site-specific generation of apoptosis-inducing hydroxyl radicals. Transformed cells (early s...
Source: Journal of Inorganic Biochemistry - November 10, 2017 Category: Biochemistry Authors: Bauer G Tags: J Inorg Biochem Source Type: research

Fe(III) - Sulfide interaction in globins: Characterization and quest for a putative Fe(IV)-sulfide species.
Abstract The present study reports findings regarding the contrast between H2S interaction with bovine hemoglobin (Hb) and horse heart myoglobin (Mb), in terms of binding and dissociation kinetics, affinities, and mechanism. At pH9.5, oxidation of ferric-sulfide adducts in presence of no free sulfide, using hexachloroiridate as oxidant is examined using stopped-flow UV-vis, EPR, vibrational spectroscopy and mass spectrometry. Oxidation of the ferric-sulfide adduct in such conditions occurs with a putative unstable Fe(IV)-sulfide adduct as intermediate that finally leads to a paramagnetic ferric species with distin...
Source: Journal of Inorganic Biochemistry - November 10, 2017 Category: Biochemistry Authors: Mot AC, Bischin C, Damian G, Attia AAA, Gal E, Dina N, Leopold N, Silaghi-Dumitrescu R Tags: J Inorg Biochem Source Type: research

Dietary luteolin protects against HgCl2-induced renal injury via activation of Nrf2-mediated signaling in rat.
Abstract Luteolin (Lut) belongs to the flavonoid family with various beneficial bioactivities. Here, we investigated whether Lut attenuate mercuric chloride (HgCl2)-induced renal injury in rat. We found that oral gavage administration of Lut (80mg/kg) alleviated anemia and renal histology upon HgCl2 treatment (80mg/L). Lut also significantly reduced HgCl2-induced oxidative stress and inflammatory, presenting as the reduced malondialdehyde (MDA) formation, increased glutathione (GSH) level, and inhibited activation of nuclear factor kappa B (NF-κB). Moreover, Lut protected renal cells from HgCl2-induced apopt...
Source: Journal of Inorganic Biochemistry - November 9, 2017 Category: Biochemistry Authors: Tan X, Liu B, Lu J, Li S, Baiyun R, Lv Y, Lu Q, Zhang Z Tags: J Inorg Biochem Source Type: research