A ruthenium(II) complex containing a p-cresol group induces apoptosis in human cervical carcinoma cells through endoplasmic reticulum stress and reactive oxygen species production.
In this study, three new luminescent Ru (II) complexes, [Ru(bpy)2(HIPMP)](ClO4)2 (Ru1, bpy = 2,2'-bipyridine, HIPMP = 2-(1H-imidazo-[4,5-f] [1,10] phenanthrolin-2-yl)-4-methylphenol), [Ru(phen)2(HIPMP)](ClO4)2 (Ru2, phen = 1,10-phenanthroline), [Ru(dmb)2(HIPMP)](ClO4)2 (Ru3, dmb = 4,4'-dimethyl-2,2'-bipyridine), were synthesized, and their anticancer activities were examined. All three complexes displayed anticancer activities against various cancer cells, with Ru2 exhibiting the highest cytotoxic activities. Ru2 was shown to accumulate specifically in the endoplasmic reticulum (ER) and induce ER stress-med...
Source: Journal of Inorganic Biochemistry - November 28, 2018 Category: Biochemistry Authors: Xu L, Zhang PP, Fang XQ, Liu Y, Wang JQ, Zhou HZ, Chen ST, Chao H Tags: J Inorg Biochem Source Type: research

Engineering of pectin-dopamine nano-conjugates for carrying ruthenium complex: A potential tool for biomedical applications.
Abstract In this work, we designed polysaccharide-metal complex of dopamine-modified pectin linking ruthenium compound, which exhibited a certain inhibition specificity to human renal cell adenocarcinoma cell line 786-O. The chemical structure and physical properties of the polysaccharide-metal complex were well characterized by multiple analysis methods. The multi-spectral results revealed that pectin-dopamine have been successfully coordinated by ruthenium complex to form nano-conjugates, which self-assembled into relatively regular nanospheres of approximately 200 nm. The polysaccharide-metal complex has more...
Source: Journal of Inorganic Biochemistry - November 28, 2018 Category: Biochemistry Authors: Diao J, Bai F, Wang Y, Han Q, Xu X, Zhang H, Luo Q, Wang Y Tags: J Inorg Biochem Source Type: research

Redox dyshomeostasis in the experimental chronic hepatic overloads with iron or copper.
ris A, Repetto MG Abstract Male rats of 80-90 g were overloaded with either Fe(II) or Cu(II) for 42 days by high concentrations of FeCl2 or CuSO4 in the drinking water. The animals were fed with a commercial rodent diet of 2780 kcal/100 g. Both metal treatments led to a liver redox imbalance and dyshomeostasis with oxidative stress and damage and the concomitant enhancement of oxidative processes as indicated by in vivo surface liver chemiluminescence, the sensitive and organ non-invasive assay for oxidative free radical reactions, and by ex vivo determined processes of phospholipid peroxidation and protei...
Source: Journal of Inorganic Biochemistry - November 23, 2018 Category: Biochemistry Authors: Musacco Sebio R, Ferrarotti N, Saporito Magriñá C, Fuda J, Torti H, Lairión F, Boveris A, Repetto MG Tags: J Inorg Biochem Source Type: research

Novel structures of platinum complexes bearing N ‑bisphosphonates and study of their biological properties.
Novel structures of platinum complexes bearing N‑bisphosphonates and study of their biological properties. J Inorg Biochem. 2018 Nov 18;191:112-118 Authors: Alvarez-Valdes A, Matesanz AI, Perles J, Fernandes C, Correia JDG, Mendes F, Quiroga AG Abstract Novel bisphosphonate platinum complexes: [Pt(isopropylamine)2(BP)]NO3 (BP = pamidronate and alendronate) have been synthesized and characterized. Their monomeric structure contains a bisphosphonate acting as chelate ligand through its oxygen atom donors, conferring the compound's cationic structure with a good solubility in water. The study of th...
Source: Journal of Inorganic Biochemistry - November 18, 2018 Category: Biochemistry Authors: Alvarez-Valdes A, Matesanz AI, Perles J, Fernandes C, Correia JDG, Mendes F, Quiroga AG Tags: J Inorg Biochem Source Type: research

Multi-action Pt(IV) anticancer agents; do we understand how they work?
Abstract Pt(IV) complexes act as prodrugs that are activated inside cancer cells releasing cytotoxic Pt(II) drugs such as cisplatin as well as two axial ligands. These ligands can be used to confer favorable pharmacological properties to the prodrug. They can be innocent spectators, targeting agents or bioactive moieties. When the ligands are bioactive moieties such as enzyme inhibitors or antiproliferative agents, the prodrug attacks several cellular targets at the same time acting as a multi-action prodrug. These compounds are very potent and often overcome resistance to cisplatin. Despite solid rationalization ...
Source: Journal of Inorganic Biochemistry - November 15, 2018 Category: Biochemistry Authors: Gibson D Tags: J Inorg Biochem Source Type: research

Interaction of amylin species with transition metals and membranes.
Abstract Islet Amyloid Polypeptide (IAPP), also known as amylin, is a 37-amino-acid peptide hormone that is secreted by pancreatic islet β-cells. Amylin is complementary to insulin in regulating and maintaining blood glucose levels in the human body. The misfolding and aggregation of amylin is primarily associated with type 2 diabetes mellitus, which is classified as an amyloid disease. Recently, the interactions between amylin and specific metal ions, e.g., copper(II), zinc(II), and iron(II), were found to impact its performance and aggregation processes. Therefore, the focus in this review will be on how th...
Source: Journal of Inorganic Biochemistry - November 10, 2018 Category: Biochemistry Authors: Alghrably M, Czaban I, Jaremko Ł, Jaremko M Tags: J Inorg Biochem Source Type: research

Carbon monoxide sensitizes cisplatin-resistant ovarian cancer cell lines toward cisplatin via attenuation of levels of glutathione and nuclear metallothionein.
Abstract Cisplatin resistance remains a major impediment to effective treatment of ovarian cancer. Despite initial platinum responsiveness, thiol-containing peptides and proteins, glutathione (GSH) and metallothionein (MT), bind and inactivate cisplatin in cancer cells. Indeed, high levels of GSH and MT in ovarian cancers impart cisplatin resistance and are predictive of poor prognosis. Cystathionine β-synthase (CBS), an enzyme involved in sulfur metabolism, is overexpressed in ovarian cancer tissues and is itself associated with cisplatin resistance. Treatment with exogenous carbon monoxide (CO), a known inh...
Source: Journal of Inorganic Biochemistry - November 10, 2018 Category: Biochemistry Authors: Kawahara B, Ramadoss S, Chaudhuri G, Janzen C, Sen S, Mascharak PK Tags: J Inorg Biochem Source Type: research

Structure-activity relationships for highly potent half-sandwich organoiridium(III) anticancer complexes with C^N-chelated ligands.
Abstract We herein report the synthesis, characterization, catalytic ability in converting coenzyme NADH to NAD+ and anticancer activity of half-sandwich iridium(III) complexes, [(η5-Cpxbiph)Ir(C^N)Cl]PF6-, where Cpxbiph = tetramethyl(biphenyl)cyclopentadienyl, C^N = varying imine-N-heterocyclic carbene ligands. The molecular structure of [(η5-Cpxbiph)Ir(L6)Cl]PF6 (complex Ir6), exhibiting the familiar "piano-stool" geometry, has been authenticated by X-ray crystallography. The anticancer activities of these complexes can be governed via substituent effects of three tunable domains and th...
Source: Journal of Inorganic Biochemistry - November 10, 2018 Category: Biochemistry Authors: Yang Y, Guo L, Ge X, Shi S, Gong Y, Xu Z, Zheng X, Liu Z Tags: J Inorg Biochem Source Type: research

Chlorogenic acid protects against aluminum toxicity via MAPK/Akt signaling pathway in murine RAW264.7 macrophages.
Abstract Aluminum (Al), which may bring about damage to the macrophages, has been implicated in the development of immunological diseases. It has been reported that chlorogenic acid (CGA, 5‑caffeoylquinic acid, chemical formula: C16H18O9) is a natural antioxidant and chelating agent with the capacity against Al (III)-induced biotoxicity. The present study was carried out to investigate whether CGA could reduce AlCl3-induced cellular damage in RAW264.7 cells. After treatment with AlCl3, the inhibition rate of viability and phagocytic activity of RAW264.7 cells was 54.5% and 27.6%, respectively. Administration of ...
Source: Journal of Inorganic Biochemistry - November 7, 2018 Category: Biochemistry Authors: Cheng D, Zhang X, Tang J, Kong Y, Wang X, Wang S Tags: J Inorg Biochem Source Type: research

The 20th International Conference on Cytochrome P450: Biochemistry, Biophysics and Biotechnology (ICCP450 2017) was held August 27-31, 2017 in D üsseldorf, Germany.
The 20th International Conference on Cytochrome P450: Biochemistry, Biophysics and Biotechnology (ICCP450 2017) was held August 27-31, 2017 in Düsseldorf, Germany. J Inorg Biochem. 2018 Nov;188:A1-A2 Authors: Urlacher VB PMID: 30227953 [PubMed - in process] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - September 21, 2018 Category: Biochemistry Authors: Urlacher VB Tags: J Inorg Biochem Source Type: research

Zeise's salt as powerful platinating agent for proteins investigated by top-down-mass spectrometry.
This study is geared towards shedding light on the biological reactivity of this platinum complex. Mass Spectrometry tools were used to obtain a deeper understanding of its interactions with biomolecules on the molecular level. Angiotensin I and Ubiquitin were chosen as model systems. Comparison to Cisplatin show that Zeise's salt is more reactive towards nucleophilic sites in proteins. Our data indicate that the ethylene ligand remains on the platinum when coordinated to a nitrogen donor in the biomolecule and therefore offers a linkage for the introduction of further functionality. When attached to sulfur donors in the b...
Source: Journal of Inorganic Biochemistry - September 8, 2018 Category: Biochemistry Authors: Cziferszky M, Gust R Tags: J Inorg Biochem Source Type: research

Study on the detoxification mechanisms to 5,10,15,20-tetrakis (4-sulfonatophenyl) porphyrinato iron(III) chloride (FeTPPS), an efficient pro-oxidant of heme water-soluble analogue.
Abstract 5,10,15,20-Tetrakis (4-sulfonatophenyl) porphyrinato iron(III) chloride (FeTPPS) is a water-soluble analog of heme and widely employed as peroxynitrite scavenger in vivo. However, previous studies have showed that like heme, FeTPPS could also act as an effective pro-oxidant towards appreciable substrates in vitro in the presence of oxidant. The reason that FeTPPS did not show any pro-oxidative damage in previous studies when it was used as peroxynitrite decomposition catalyst in vivo, has not been studied. Herein, the effects of two main detoxification mechanisms of heme, i.e., serum albumin (SA) binding ...
Source: Journal of Inorganic Biochemistry - September 6, 2018 Category: Biochemistry Authors: Zhang P, Ma L, Yang Z, Li H, Gao Z Tags: J Inorg Biochem Source Type: research

6th Georgian Bay International Conference on Bioinorganic Chemistry.
PMID: 30078467 [PubMed - in process] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - August 8, 2018 Category: Biochemistry Authors: Stillman M, Kobayashi N, Dawson J, Ding Z Tags: J Inorg Biochem Source Type: research

Mechanism of hydrolysis of a platinum(IV) complex discovered by atomic telemetry.
te; J Abstract Herein we report on the hydrolysis mechanism of [Pt{N(p-HC6F4)CH2}2(NC5H5)2(OH)2], a platinum(IV) complex that exhibits anti-cancer properties. Atomic telemetry, an in situ technique based on electron structure sensitive X-ray spectroscopy, revealed that hydrolysis preceded any reduction of the metal center. The obtained results are complemented with 19F NMR measurements and theoretical calculations and support the observation that this PtIV complex does not reduce spontaneously to PtII in HEPES buffer solution at pH 7.4 and after 24 h incubation. These results are of importance for the design o...
Source: Journal of Inorganic Biochemistry - July 24, 2018 Category: Biochemistry Authors: Czapla-Masztafiak J, Kubas A, Kayser Y, Fernandes DLA, Kwiatek WM, Lipiec E, Deacon GB, Al-Jorani K, Wood BR, Szlachetko J, Sá J Tags: J Inorg Biochem Source Type: research

Complexes in aqueous cobalt(II)-2-picolinehydroxamic acid system: Formation equilibria, DNA-binding ability, antimicrobial and cytotoxic properties.
Abstract The coordination properties of 2-picolinehydroxamic acid towards cobalt(II) in aqueous solution were determined by a pH-metric method and confirmed by spectroscopic (UV-Vis and ESI-MS) studies. The results show the formation of mononuclear complexes, as well as of metallacrowns (MC). All methods indicate a high tendency of 2-picolinehydroxamic acid to form cobalt(II) metallacrown 12-MC-4. ESI-MS additionally confirms 15-MC-5 and 18-MC-6, stabilized by a sodium ion and methanol. The complexes observed in the speciation model at a pH about 7.2 were studied for their DNA-binding ability. The decrease of abso...
Source: Journal of Inorganic Biochemistry - July 24, 2018 Category: Biochemistry Authors: Woźniczka M, Świątek M, Pająk M, Gądek-Sobczyńska J, Chmiela M, Gonciarz W, Lisiecki P, Pasternak B, Kufelnicki A Tags: J Inorg Biochem Source Type: research

New heterobimetallic ferrocenyl derivatives: Evaluation of their potential as prospective agents against trypanosomatid parasites and Mycobacterium tuberculosis.
ino D Abstract Searching for prospective agents against infectious diseases, four new ferrocenyl derivatives, [M(L)(dppf)4](PF6), with M = Pd(II) or Pt(II), dppf = 1,1'-bis(dipheny1phosphino) ferrocene and HL = tropolone (HTrop) or hinokitiol (HHino), were synthesized and characterized. Complexes and ligands were evaluated against the bloodstream form of T. brucei, L. infantum amastigotes, M. tuberculosis (MTB) sensitive strain and MTB clinical isolates. Complexes showed a significant increase of the anti-T. brucei activity with respect to the free ligands (>28- and>46-fold for Trop and 6- and 22...
Source: Journal of Inorganic Biochemistry - July 23, 2018 Category: Biochemistry Authors: Rivas F, Medeiros A, Rodríguez Arce E, Comini M, Ribeiro CM, Pavan FR, Gambino D Tags: J Inorg Biochem Source Type: research

In vitro and in silico study of the biological activity of manganese(III) inverse-[9-MC-3]-metallacrowns and manganese(II) complexes with the anti-inflammatory drugs diclofenac or indomethacin.
Abstract In the present contribution, the biological properties of four manganese complexes with the non-steroidal anti-inflammatory drugs sodium diclofenac (Nadicl) or indomethacin (Hindo) in the presence or absence of salicylaldoxime (Η2sao), i.e. [Μn6(O)2(dicl)2(sao)6(CH3OH)6] 1, [Μn6(O)2(indo)2(sao)6(H2O)4], 2, [Μn(dicl)2(CH3OH)4], 3, and [Μn(indo)2(CH3OH)4], 4 are presented. More specifically, the in vitro cytotoxic effects of the complexes were evaluated against three cancer cell lines (HeLa, MCF-7 and A549 cells) as well as their combinatory activity with the well-known chemotherapeutic drug...
Source: Journal of Inorganic Biochemistry - July 21, 2018 Category: Biochemistry Authors: Geromichalos GD, Tarushi A, Lafazanis K, Pantazaki AA, Kessissoglou DP, Psomas G Tags: J Inorg Biochem Source Type: research

Syntheses and biological studies of nitroimidazole conjugated heteroscorpionate ligands and related Cu(I) and Cu(II) complexes.
Abstract Copper(I) and copper(II) complexes of 5-nitroimidazole conjugated heteroscorpionate ligands have been synthesized. In particular, the new 2,2-bis(pyrazol-1-yl)-N-(2-(2-methyl-5-nitro-1H-imidazol-1-yl)ethyl)acetamide ligand (LHMN) was synthesized by direct coupling of preformed side chain acid with 5-nitroimidazole and its coordination chemistry was investigated towards Cu(I) and Cu(II) acceptors and compared with that of the related 2,2-bis(3,5-dimethyl-1-H-pyrazol-1-yl)-N-(2-(2-methyl-5-nitro-1H-imidazol-1-yl)ethyl)acetamide ligand (LMeMN). The copper(II) complexes {[(LMeMN)2Cu]Cl2} and {[(LHMN)2Cu]Cl2} ...
Source: Journal of Inorganic Biochemistry - July 19, 2018 Category: Biochemistry Authors: Pellei M, Gandin V, Cimarelli C, Quaglia W, Mosca N, Bagnarelli L, Marzano C, Santini C Tags: J Inorg Biochem Source Type: research

Low Ctr1p, due to lack of Sco1p results in lowered cisplatin uptake and mediates insensitivity of rho0 yeast to cisplatin.
We report here that low copper medium rendered wild type hypersensitive to cisplatin, but failed to sensitize rho0 yeast to cisplatin. Wild type yeast grown in low copper medium exhibited ~2.0 fold enhanced cytotoxicity in survival and colony-forming ability compared to copper adequate wild type cells. The effect of copper restriction on cisplatin sensitivity was associated with upregulation of copper transporter 1 mRNA as well as protein, facilitating enhanced uptake and accumulation of cisplatin. Rho0 yeast also showed increased copper transporter 1 mRNA upon copper restriction, but failed to increase corresponding prote...
Source: Journal of Inorganic Biochemistry - July 19, 2018 Category: Biochemistry Authors: Bodiga S, Vemuri PK, Bodiga VL Tags: J Inorg Biochem Source Type: research

Excessive zinc chloride induces murine photoreceptor cell death via reactive oxygen species and mitochondrial signaling pathway.
Abstract Zinc (Zn) is a fundamental trace element for cell viability and physiology, and concentrations above the physiological level may lead to cell damage. In order to explore the effect of zinc chloride (ZnCl2) on murine photoreceptor cells, in this study, we investigated the alterations of murine photoreceptor cell proliferation and cell morphology after exposure to various concentrations of ZnCl2, determined the levels of hydrogen peroxide, hydroxyl radicals, cytochrome c, and ATP before and after cells exposure to different concentrations of ZnCl2, monitored the changes of mitochondrial membrane potential, ...
Source: Journal of Inorganic Biochemistry - July 19, 2018 Category: Biochemistry Authors: Wang H, Liu B, Yin X, Guo L, Jiang W, Bi H, Guo D Tags: J Inorg Biochem Source Type: research

Integrin αvβ3 receptor targeting PET/MRI dual-modal imaging probe based on the 64Cu labeled manganese ferrite nanoparticles.
Integrin αvβ3 receptor targeting PET/MRI dual-modal imaging probe based on the 64Cu labeled manganese ferrite nanoparticles. J Inorg Biochem. 2018 Jul 06;186:257-263 Authors: Shi X, Shen L Abstract With the advent of positron emission tomography/magnetic resonance imaging (PET/MRI) scanner, PET/MRI dual-modal imaging will play more and more important role in the diagnosis of cancers and other diseases. Until now, there is no an approved PET/MRI dual-modal imaging probe. The goal of this work is to design and synthesize potential PET/MRI dual-modal imaging probe based on superparamagnetic ma...
Source: Journal of Inorganic Biochemistry - July 6, 2018 Category: Biochemistry Authors: Shi X, Shen L Tags: J Inorg Biochem Source Type: research

Inhibitory effect of melittin on endonuclease-like activity of centrin.
Abstract The xeroderma pigmentosum group C protein (XPC) and centrin2 are the primary initiators of global genome nucleotide excision repair (NER). Centrin, acts as a member of the EF-hand super family of calcium-binding proteins, playing roles in reconstitution of the vitro NER reaction. To understand the possible molecular and structural properties of the multiprotein process, the interactions of Euplotes octocarinatus centrin (EoCen), melittin, and DNA are described. EoCen shares a sequence identity of 66% with centrin2. Melittin possesses inverse direction hydrophobic triads-leucine-leucine-tryptophan (LLW) wh...
Source: Journal of Inorganic Biochemistry - July 5, 2018 Category: Biochemistry Authors: Zhang W, Shi E, Zhao Y, Yang B Tags: J Inorg Biochem Source Type: research

Cytotoxicity, dual-targeting apoptosis induction evaluation of multinuclear cu complexes based on pyrazine-benzimidazole derivative.
Abstract To investigate the cytotoxicity and mechanism of action of multinuclear Cu complexes against tumor cell lines, two complexes, Cu6(bpbib)4Br8 (1) and Cu2(bpbib)2(BF4)2Cl2 (2) (bpbib = 1,4-bis((2-(pyrazin-2-yl)-1H-benzo[d]imidazol-1-yl)methyl)benzene) were synthesized and characterized. Both Cu complexes showed high selectivity toward cancer and not normal cells, and the SMMC7721 cell line showed most sensitivity toward both complexes. Complex 1 exhibited more potent cytotoxicity and enhanced cellular uptake, and therefore, was comprehensively investigated. Complex 1 exhibited dual effects in the inhibi...
Source: Journal of Inorganic Biochemistry - June 30, 2018 Category: Biochemistry Authors: Hu J, Chen S, Mao R, Liao C, Yang H, Zhao J Tags: J Inorg Biochem Source Type: research

Magnetic nanoparticles as a support for a copper (II) complex with nuclease activity.
s ICN Abstract Magnetic nanoparticles have been extensively explored for the development of platforms for drug delivery and imaging probes. In this work, we have used a modular capping strategy to produce magnetic gold-coated Fe3O4 (Fe3O4@Au) nanoparticles, which have been decorated with a copper (II) complex containing a thioether derivative of clip-phen (Fe3O4@Au@Cu), where the complex [Cu(2CP-Bz-SMe)]2+ has affinity to bind DNA and proven nuclease activity (2CP-Bz-SMe=1,3-bis((1,10-phenanthrolin-2-yl)oxy)-N-(4-(methylthio)benzylidene)propan-2-imine). The functionalization of Fe3O4@Au with the copper complex occ...
Source: Journal of Inorganic Biochemistry - June 30, 2018 Category: Biochemistry Authors: Silva MAS, Romo AIB, Abreu DS, Carepo MSP, Lemus L, Jafelicci M, Paulo TF, Nascimento OR, Vargas E, Denardin JC, Diógenes ICN Tags: J Inorg Biochem Source Type: research

MicroRNA-193b-3p regulates hepatocyte apoptosis in selenium-deficient broilers by targeting MAML1.
Abstract Selenium (Se) is an important nutritional element in the diet. Apoptosis is one of the characteristic pathological changes in liver tissue resulting from Se deficiency. MicroRNA (miRNA) plays an important role in cell proliferation, differentiation, apoptosis and tumorigenesis. However, why apoptosis occurs during Se deficiency and how miRNA regulates hepatocyte apoptosis in broilers requires further study. We used a dual luciferase reporter assay system and quantitative real-time PCR (qPCR) to screen hepatocytes in Se-deficient broilers for the specificity of hepatocyte apoptosis miRNA and its target pro...
Source: Journal of Inorganic Biochemistry - June 28, 2018 Category: Biochemistry Authors: Liu T, Yang T, Xu Z, Tan S, Pan T, Wan N, Li S Tags: J Inorg Biochem Source Type: research

Magnetic resonance imaging contrast enhancement in vitro and in vivo by octanuclear iron-oxo cluster-based agents.
acute;ndez R, Raptis RG Abstract A water-soluble octanuclear cluster, [Fe8], was studied with regard to its properties as a potential contrast enhancing agent in magnetic resonance imaging (MRI) in magnetic fields of 1.3, 7.2 and 11.9 T and was shown to have transverse relaxivities r2 = 4.01, 10.09 and 15.83 mM s-1, respectively. A related hydrophobic [Fe8] cluster conjugated with 5 kDa hyaluronic acid (HA) was characterized by 57Fe-Mössbauer and MALDI-TOF mass spectroscopy, and was evaluated in aqueous solutions in vitro with regard to its contrast enhancing properties [r2 = 3.65 mM s-1 (...
Source: Journal of Inorganic Biochemistry - June 24, 2018 Category: Biochemistry Authors: Das S, Parga K, Chakraborty I, Tinoco AD, Delgado Y, López PM, Fernández Vega L, Sanakis Y, Ghosh S, Bankson J, Klostergaard J, González-Méndez R, Raptis RG Tags: J Inorg Biochem Source Type: research

Hydrolysis reaction promotes changes in coordination mode of Ru(II)/acylthiourea organometallic complexes with cytotoxicity against human lung tumor cell lines.
In this study, Ru(II)-arene complexes with acylthiourea ligands of the type [Ru(η6‑p‑cymene)(PPh3)(T)Cl]PF6(1-5) and [Ru(η6‑p‑cymene)(PPh3)(T)]PF6(1a, 4a), where PPh3 = triphenylphosphine and T = N‑acyl‑N'(monosubstituted)thiourea, were synthesized and characterized, and their cytotoxic properties were also evaluated. 1a and 4a were obtained from the hydrolysis reaction of 1 and 4. All complexes showed unusual coordination modes for acylthiourea ligands, which are coordinated in a monodentate fashion (S) in 1-5, while they found to be bidentate (S,N), in 1a and 4a. To the best of our knowledge, ...
Source: Journal of Inorganic Biochemistry - June 18, 2018 Category: Biochemistry Authors: Cunha BN, Colina-Vegas L, Plutín AM, Silveira RG, Honorato J, Oliveira KM, Cominetti MR, Ferreira AG, Castellano EE, Batista AA Tags: J Inorg Biochem Source Type: research

Selective Cu(I) complex with phosphine-peptide (SarGly) conjugate contra breast cancer: Synthesis, spectroscopic characterization and insight into cytotoxic action.
Abstract The main disadvantage of conventional anticancer chemotherapy is the inability to deliver the correct amount of drug directly to cancer. Those molecular delivering systems are very important to destroy cancer cells selectively. Herein we report synthesis of phosphine-peptide conjugate (Ph2PCH2-Sar-Gly-OH, PSG) derived from SarGly (sarcosine-glycine), which can be easily exchanged to other peptide carriers, its oxide (OPh2PCH2-Sar-Gly-OH, OPSG) and the first copper(I) complex ([CuI(dmp)(P(Ph)2CH2-Sar-Gly-OH)], 1-PSG, where dmp stands for 2,9-dimethyl-1,10-phenanthroline). The compounds were characterized b...
Source: Journal of Inorganic Biochemistry - June 18, 2018 Category: Biochemistry Authors: Komarnicka UK, Kozieł S, Starosta R, Kyzioł A Tags: J Inorg Biochem Source Type: research

Interactions between metal binding viral proteins and human targets as revealed by network-based bioinformatics.
Abstract The role of metal ions in virus-host interactions is largely unknown. In the present computational study, the putative zinc, manganese, magnesium, and iron metalloproteomes of 226 different viruses were identified and then the human proteins interacting the viral metalloproteomes were retrieved through network-based bioinformatics. Protein features such as abundance, function and structurally disorder were classified according to the metal type bound to viral proteins. Likely perturbations of host cellular metal homeostasis by viral metal binders are also discussed. Furthermore, possible molecular linkage...
Source: Journal of Inorganic Biochemistry - June 15, 2018 Category: Biochemistry Authors: Chasapis CT Tags: J Inorg Biochem Source Type: research

Cross-linked cytochrome P450 BM3 aggregates promoted by Ru(II)-diimine complexes bearing aldehyde groups.
Abstract Cross-linked enzyme aggregate (CLEA) methodology has been applied to immobilize cytochrome P450 BM3 variants (F87A and 21B3) with peroxygenase activity. Several Ru(II)-diimine complexes were found to be suitable cross-linking agents, surpassing the traditional glutaraldehyde and dextran aldehyde. They offer modular numbers of aldehyde functionalities and a more rigid framework than their organic counterparts. The F87A CLEAs display significant activity loss compared to the protein in solution. Meanwhile, for the 21B3 CLEAs, high activity recovery (up to 95%) is obtained. In order to minimize enzyme leachi...
Source: Journal of Inorganic Biochemistry - June 5, 2018 Category: Biochemistry Authors: Do MQ, Henry E, Kato M, Cheruzel L Tags: J Inorg Biochem Source Type: research

Bifunctional 3-hydroxy-4-pyridinones as effective aluminium chelators: synthesis, solution equilibrium studies and in vivo evaluation.
Abstract This paper reports the results on the study of a set of synthesized bifunctional 3-hydroxy-4-pyridinones chelators as potential aluminium sequestering agents. They were N-functionalized with alkyl-amino, -carboxylic and -(amino-carboxylic) groups, envisaging the improvement of the Al3+ sequestering capacity, in comparison with the marketed chelating drug deferiprone. The main focus of this work was given to the assessment of their binding ability towards Al3+, which was studied by potentiometric and UV-Vis spectrophotometric measurements carried out at T = 298.15 K. The speciation models were charac...
Source: Journal of Inorganic Biochemistry - May 30, 2018 Category: Biochemistry Authors: Irto A, Cardiano P, Chand K, Cigala RM, Crea F, De Stefano C, Gano L, Sammartano S, Santos MA Tags: J Inorg Biochem Source Type: research

Determining the glycation site specificity of human holo-transferrin.
, Rangel M Abstract Understanding the effect of glycation on the function of transferrin, the systemic iron transporter, is fundamental to fully grasp the mechanisms leading to the loss of iron homeostasis observed in diabetes mellitus (DM). The spontaneous reaction with protein amino groups is one of the main causes of glucose toxicity, but the site specificity of this reaction is still poorly understood. Here in, an in vitro approach was used to study human holo-transferrin glycation in detail. Lysine residues 103, 312 and 380 proved to be the most reactive sites, and overall glycation specificity was found to b...
Source: Journal of Inorganic Biochemistry - May 26, 2018 Category: Biochemistry Authors: Silva AMN, Coimbra JTS, Castro MM, Oliveira Â, Brás NF, Fernandes PA, Ramos MJ, Rangel M Tags: J Inorg Biochem Source Type: research

Binding properties of chiral ruthenium(II) complexes Λ- and Δ-[Ru(bpy)2dppz-11-CO2Me]2+ toward the triplex RNA poly(U)•poly(A)*poly(U).
Binding properties of chiral ruthenium(II) complexes Λ- and Δ-[Ru(bpy)2dppz-11-CO2Me]2+ toward the triplex RNA poly(U)•poly(A)*poly(U). J Inorg Biochem. 2018 May 24;186:51-59 Authors: Ni W, Liu X, Tan L Abstract Two chiral ruthenium(II) complexes containing ligand dppz-CO2Me (dppz-11-CO2Me = dipyrido[3,2-a,2',3'-c]phenazine-11-carboxylic acid methyl ester), Δ-[Ru(bpy)2dppz-11-CO2Me]2+ (bpy = 2,2'-bipyridine; Δ-1) and Λ-[Ru(bpy)2dppz-11-CO2Me]2+ (Λ-1), were synthesized and characterized. The binding of the two enantiomers with the triplex RNA poly(U)&...
Source: Journal of Inorganic Biochemistry - May 24, 2018 Category: Biochemistry Authors: Ni W, Liu X, Tan L Tags: J Inorg Biochem Source Type: research

The trans-[Ru(PPh3)2(N,N-dimethyl-N'-thiophenylthioureato-k2O,S)(bipy)]PF6 complex has pro-apoptotic effects on triple negative breast cancer cells and presents low toxicity in vivo.
Abstract Triple negative breast cancer (TNBC) is a heterogeneous subtype of breast tumors that does not exhibit the expression of estrogen and progesterone receptors, neither the amplification of the human epidermal growth factor receptor 2 (HER-2) gene. Despite all the advances in cancer treatments, the development of new anticancer drugs for TNBC tumors is still a challenge. There is an increasing interest in new agents to be used in cancer treatment. Ruthenium is a metal that has unique characteristics and important in vivo and in vitro results achieved for cancer treatment. Thus, in this work, with the aim to ...
Source: Journal of Inorganic Biochemistry - May 24, 2018 Category: Biochemistry Authors: Becceneri AB, Popolin CP, Plutin AM, Maistro EL, Castellano EE, Batista AA, Cominetti MR Tags: J Inorg Biochem Source Type: research

Gallium(III) complexes of α-N-heterocyclic piperidylthiosemicarbazones: Synthesis, structure-activity relationship, cellular uptake and activation of caspases-3/7/9.
Gallium(III) complexes of α-N-heterocyclic piperidylthiosemicarbazones: Synthesis, structure-activity relationship, cellular uptake and activation of caspases-3/7/9. J Inorg Biochem. 2018 May 23;186:42-50 Authors: Qi J, Yao Q, Qian K, Tian L, Cheng Z, Wang Y Abstract Two types of α-N-heterocyclic piperidylthiosemicarbazone ligands and related Ga(III) complexes were synthesized. The structure of Ga4 and Ga5 were characterized by X-ray single crystal diffraction. We generated the related α-N-heterocycliperidinylthiosemicarbazone analogs to examine the effect of aldehydes or ketones in ...
Source: Journal of Inorganic Biochemistry - May 23, 2018 Category: Biochemistry Authors: Qi J, Yao Q, Qian K, Tian L, Cheng Z, Wang Y Tags: J Inorg Biochem Source Type: research

Combination of 7-hydroxycoumarin in a platinum(IV) complex derived from cisplatin enhanced cytotoxicity with multiple mechanisms of action.
Abstract A novel compound, Cou-platin, composed of 7-hydroxycoumarin and a platinum(IV) moiety derived from cisplatin was designed and synthesized. Significantly, Cou-platin exhibited more potent in vitro antitumor activity against all tested cancer cell lines than that of cisplatin, which was mainly attributed to the liberation of cisplatin and 7-hydroxycoumarin upon reduction with a biomolecular agent. Besides, cellular accumulation of Cou-platin was dramatically increased among several cancer cells in contrast to cisplatin. Flow cytometry study revealed that Cou-platin arrested cell cycle at G2 phase and induce...
Source: Journal of Inorganic Biochemistry - May 23, 2018 Category: Biochemistry Authors: Hua W, Zhao J, Hu W, Gou S Tags: J Inorg Biochem Source Type: research

Interaction of 17 α-hydroxylase, 17(20)-lyase (CYP17A1) inhibitors - abiraterone and galeterone - with human sterol 14α-demethylase (CYP51A1).
Interaction of 17α-hydroxylase, 17(20)-lyase (CYP17A1) inhibitors - abiraterone and galeterone - with human sterol 14α-demethylase (CYP51A1). J Inorg Biochem. 2018 May 21;186:24-33 Authors: Masamrekh R, Kuzikov A, Veselovsky A, Toropygin I, Shkel T, Strushkevich N, Gilep A, Usanov S, Archakov A, Shumyantseva V Abstract Abiraterone and galeterone induce type I differential spectral changes in human sterol 14α-demethylase (cytochrome P450 51A1, CYP51A1) with the sigmoidal shape of the binding curve. After approximation of the data by Hill model, the half-saturation concentrations (K0.5...
Source: Journal of Inorganic Biochemistry - May 21, 2018 Category: Biochemistry Authors: Masamrekh R, Kuzikov A, Veselovsky A, Toropygin I, Shkel T, Strushkevich N, Gilep A, Usanov S, Archakov A, Shumyantseva V Tags: J Inorg Biochem Source Type: research

Collagen promotes matrix vesicle-mediated mineralization by vascular smooth muscle cells.
Abstract Vascular calcification (VC) is a hallmark of atherosclerotic plaques. Calcification of advanced plaques shares common features with endochondral ossification of long bones and appears to be protective. On the other hand, microcalcification of early plaques, which is poorly understood, is thought to be harmful. Tissue-nonspecific alkaline phosphatase (TNAP) and collagen are the two proteins necessary for physiological mineralization. Here, we demonstrate the presence of membrane-bound TNAP, detected by immunofluorescence, that seems to form clusters on the plasma membrane of vascular smooth muscle cells (V...
Source: Journal of Inorganic Biochemistry - May 18, 2018 Category: Biochemistry Authors: Roszkowska M, Strzelecka-Kiliszek A, Bessueille L, Buchet R, Magne D, Pikula S Tags: J Inorg Biochem Source Type: research

Functional modeling of the MnCAT active site with a dimanganese(III) complex of an unsymmetrical polydentate N3O3 ligand.
gnorella SR Abstract A new diMnIII complex, [Mn2L(OAc)2(H2O)](BPh4)·3H2O (1), obtained with the unsymmetrical N3O3-ligand H3L = 1-[N-(2-pyridylmethyl),N-(2-hydroxybenzyl)amino]-3-[N'-(2-hydroxybenzyl),N'-(benzyl)amino]propan-2-ol, has been prepared and characterized. The unsymmetrical hexadentate ligand L3- leads to coordination dissymmetry (dissimilar donor atoms) around each Mn ion (N2O4 and NO4(solvent), respectively) leaving one labile site on one of the two Mn ions that facilitates interaction of the metal center with H2O2, as in Mn catalase. 1 is able to catalyze H2O2 disproportionation in acetoni...
Source: Journal of Inorganic Biochemistry - May 18, 2018 Category: Biochemistry Authors: Ledesma GN, Anxolabéhère-Mallart E, Sabater L, Hureau C, Signorella SR Tags: J Inorg Biochem Source Type: research

A survey of methionine-aromatic interaction geometries in the oxidoreductase class of enzymes: What could Met-aromatic interactions be doing near metal sites?
Abstract Redox reactions of the aromatic amino acids tyrosine (Tyr) and tryptophan (Trp) are crucial for the biological functions of many metalloproteins. An important question is how biological systems can use the protein environment to move electrons through proteins in a controlled manner. Methionine (Met)-aromatic interactions are common in proteins, but little is known about redox reactions of such motifs. Here, we explore methionine sulfur-aromatic interactions in the oxidoreductase (EC 1) class of proteins and their proximity to metal sites. We also propose a new metric for classifying Met-aromatic interact...
Source: Journal of Inorganic Biochemistry - May 18, 2018 Category: Biochemistry Authors: Weber DS, Warren JJ Tags: J Inorg Biochem Source Type: research

Cationic Au(I) complexes with aryl-benzothiazoles and their antibacterial activity.
Abstract Two cationic Au(I) complexes derived from aryl-benzothiazoles, namely [(PPh3)Au(pbt)](OTf) (1) and [(PPh3)Au(qbt)](OTf) (2) (where pbt = 2‑(pyridyl)benzothiazole and qbt = (quinolyl)benzothiazole, and OTf- = trifluoromethanesulfonate anion), have been synthesized and structurally characterized by X-ray crystallography. Both complexes exhibit strong antibacterial effects against Gram-negative bacteria such as Acinetobacter baumannii and Pseudomonas Aeruginosa. Results of examination of the reactions of 1 and 2 indicate that these cationic Au(I) complexes rapidly cross the bacterial membrane a...
Source: Journal of Inorganic Biochemistry - May 11, 2018 Category: Biochemistry Authors: Stenger-Smith J, Chakraborty I, Mascharak PK Tags: J Inorg Biochem Source Type: research

How does binuclear zinc amidohydrolase FwdA work in the initial step of methanogenesis: From formate to formyl-methanofuran.
Abstract The initial step of methanogenesis is the fixation of CO2 to formyl-methanofuran (formyl-MFR) catalyzed by formyl-MFR dehydrogenase, which can be divided into two half reactions. Herein, the second half reaction catalyzed by FwdA (formyl-methanofuran dehydrogenase subunit A), i.e., from formate to formyl-methanofuran, has been investigated using density functional theory and a chemical model based on the X-ray crystal structure. The calculations indicate that, compared with other well-known di-zinc hydrolases, the FwdA reaction employs a reverse mechanism, including the nucleophilic attack of MFR amine on...
Source: Journal of Inorganic Biochemistry - May 11, 2018 Category: Biochemistry Authors: Zhang XW, Chen SL Tags: J Inorg Biochem Source Type: research

Effects on estrogen-dependent and triple negative breast cancer cells growth of Ni(II), Zn(II) and Cd(II) complexes with the Schiff base derived from pyridine-2-carboxaldehyde and 5,6-diamino-1,3-dimethyluracil explored through the renin-angiotensin system (RAS)-regulating aminopeptidases.
Martínez-Martos JM, Moreno-Carretero MN Abstract A series of Ni(II), Zn(II) and Cd(II) complexes with the Schiff base derived from the condensation 1:1 from pyridine-2-carboxaldehyde and 5,6-diamino-1,3-dimethyluracil (6-amino-1,3-dimethyl-5-[(pyridin-2-ylmethylidene)-amino]pyrimidine-2,4(1H,3H)-dione, DAAUPic) were synthesized and subsequently characterized by means of elemental analysis, FT-IR, NMR and nine of them by X-ray diffraction. Except the [Zn(μ-O,O'-AcO)(N5,N6,N1F-DAAUPicH-1)]2 and [Cd(O,O'-NO3)(μ-O4,(N5,N6,N1F)-DAAUPicH-1)(H2O)]2·2H2O dimers and the [Cd(μ-S,N-SCN)(N5,N6,N1F-DAAU...
Source: Journal of Inorganic Biochemistry - May 9, 2018 Category: Biochemistry Authors: Illán-Cabeza NA, Jiménez-Pulido SB, Hueso-Ureña F, Ramírez-Expósito MJ, Sánchez-Sánchez P, Martínez-Martos JM, Moreno-Carretero MN Tags: J Inorg Biochem Source Type: research

Investigating Cytochrome P450 specificity during glycopeptide antibiotic biosynthesis through a homologue hybridization approach.
Abstract Cytochrome P450 enzymes perform an impressive range of oxidation reactions against diverse substrate scaffolds whilst generally maintaining a conserved tertiary structure and active site chemistry. Within secondary metabolism, P450 enzymes play widespread and important roles in performing crucial modifications of precursor molecules, with one example of the importance of such reactions being found in the biosynthesis of the glycopeptide antibiotics (GPAs). In GPA biosynthesis P450s, known as Oxy enzymes, are key players in the cyclization of the linear GPA peptide precursor, which is a process that is bot...
Source: Journal of Inorganic Biochemistry - May 3, 2018 Category: Biochemistry Authors: Brieke C, Tarnawski M, Greule A, Cryle MJ Tags: J Inorg Biochem Source Type: research

Novel polypyridyl ruthenium complexes acting as high affinity DNA intercalators, potent transcription inhibitors and antitumor reagents.
Abstract Six novel polypyridyl ruthenium complexes with (E)-2-styryl-1H- imidazo[4,5-f][1,10]phenanthroline ligand and its analogues have been designed to enhance the DNA intercalation ability of their model compound [Ru(bpy)2(pip)]2+ (bpy = 2,2'-bipyridine, pip = 2-phenyl-1H-imidazo[4,5-f][1,10]phenanthroline). As shown in the optimized geometry of the complexes, the introduction of styryl group not only extended the conjugated area of the intercalative ligand, but also retained the excellent planarity. These two merits have been proven to be beneficial for their DNA intercalation, thus greatly improved t...
Source: Journal of Inorganic Biochemistry - May 2, 2018 Category: Biochemistry Authors: Ma GL, Bi XD, Gao F, Feng Z, Zhao DC, Lin FJ, Yan R, Liu D, Liu P, Chen J, Zhang H Tags: J Inorg Biochem Source Type: research

Structure of cytochrome P450 2B4 with an acetate ligand and an active site hydrogen bond network similar to oxyferrous P450cam.
PMID: 29730233 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - April 30, 2018 Category: Biochemistry Authors: Yang Y, Bu W, Im S, Meagher J, Stuckey J, Waskell L Tags: J Inorg Biochem Source Type: research

Conserved cysteine residues are necessary for nickel-induced allosteric regulation of the metalloregulatory protein YqjI (NfeR) in E. coli.
Abstract Transition metal homeostasis is necessary to sustain life. First row transition metals act as cofactors within the cell, performing vital functions ranging from DNA repair to respiration. However, intracellular metal concentrations exceeding physiological requirements may be toxic. In E. coli, the YqjH flavoprotein is thought to play a role in iron homeostasis. YqjH is transcriptionally regulated by the ferric uptake regulator and a newly discovered regulator encoded by yqjI. The apo-form of YqjI is a transcriptional repressor of both the yqjH and yqjI genes. YqjI repressor function is disrupted upon bind...
Source: Journal of Inorganic Biochemistry - April 26, 2018 Category: Biochemistry Authors: Blahut M, Dzul S, Wang S, Kandegedara A, Grossoehme NE, Stemmler T, Outten FW Tags: J Inorg Biochem Source Type: research

A combined computational and experimental study on selective flucloxacillin hydroxylation by cytochrome P450 BM3 variants.
Abstract The 5'-hydroxymethyl metabolite of the penicillin based antibiotic flucloxacillin (FLX) is considered to be involved in bile duct damage occurring in a small number of patients. Because 5'-hydroxymethyl FLX is difficult to obtain by organic synthesis, biosynthesis using highly active and regioselective biocatalysts would be an alternative approach. By screening an in-house library of Cytochrome P450 (CYP) BM3 mutants, mutant M11 L437E was identified as a regioselective enzyme with relatively high activity in production of 5'-hydroxymethyl FLX as was confirmed by mass spectrometry and NMR. In contrast, inc...
Source: Journal of Inorganic Biochemistry - April 24, 2018 Category: Biochemistry Authors: Luirink RA, Dekker SJ, Capoferri L, Janssen LFH, Kuiper CL, Ari ME, Vermeulen NPE, Vos JC, Commandeur JNM, Geerke DP Tags: J Inorg Biochem Source Type: research

Modeling the hydrogen sulfide binding to heme.
Abstract The binding of hydrogen sulfide to a model heme compound is investigated by coupled-cluster singles-doubles augmented by a perturbative triple excitations, CCSD(T), and density functional theory, DFT. The minimum energy path for the H2S addition to an isolated heme center of the heme protein is evaluated by adopting as a model the heme compound FeP(Im) (P = porphyrin; Im = imidazole). The FeP(Im)-H2S aduct is bound by 13.7 kcal/mol at the CCSD(T) level of theory. Relaxed potential energy curves for the lowest lying spin states of the H2S to FeP(Im) binding using DFT reveal that the binding proce...
Source: Journal of Inorganic Biochemistry - April 21, 2018 Category: Biochemistry Authors: Ostojić BD, Schwerdtfeger P, Đorđević DS Tags: J Inorg Biochem Source Type: research

Role of the HSPA9/HSC20 chaperone pair in promoting directional human iron-sulfur cluster exchange involving monothiol glutaredoxin 5.
Abstract Iron‑sulfur clusters are essential cofactors found across all domains of life. Their assembly and transfer are accomplished by highly conserved protein complexes and partners. In eukaryotes a [2Fe-2S] cluster is first assembled in the mitochondria on the iron‑sulfur cluster scaffold protein ISCU in tandem with iron, sulfide, and electron donors. Current models suggest that a chaperone pair interacts with a cluster-bound ISCU to facilitate cluster transfer to a monothiol glutaredoxin. In humans this protein is glutaredoxin 5 (GLRX5) and the cluster can then be exchanged with a variety of target apo pro...
Source: Journal of Inorganic Biochemistry - April 11, 2018 Category: Biochemistry Authors: Olive JA, Cowan JA Tags: J Inorg Biochem Source Type: research