Dinuclear copper(II) complexes with a bridging bis(chalcone) ligand reveal considerable in vitro cytotoxicity on human cancer cells and enhanced selectivity
J Inorg Biochem. 2024 Mar;252:112481. doi: 10.1016/j.jinorgbio.2024.112481. Epub 2024 Jan 4.ABSTRACTA bis(chalcone) molecule (H2L) was synthesized via Aldol's condensation from terephthalaldehyde and 2'-hydroxyacetophenone and it was used as bridging ligand for the preparation of five dinuclear copper(II) complexes of the composition [Cu(NN)(μ-L)Cu(NN)](NO3)2⋅nH2O (n = 0-2) (1-5), where NN stands for a bidentate N-donor ligand such as phen (1,10-phenanthroline, 1), bpy (2,2'-bipyridine, 2), mebpy (5,5'-dimethyl-2,2'-dipyridine, 3), bphen (bathophenanthroline, 4) and nphen (5-nitro-1,10-phenanthroline, 5). The compounds ...
Source: Journal of Inorganic Biochemistry - January 12, 2024 Category: Biochemistry Authors: Zden ěk Trávníček J án Vančo Jan Belza Giorgio Zoppellaro Zden ěk Dvořák Source Type: research
Antitumor active trans ‑platinum complexes through metabolic stability and enhanced cellular accumulation
J Inorg Biochem. 2024 Mar;252:112475. doi: 10.1016/j.jinorgbio.2023.112475. Epub 2023 Dec 29.ABSTRACTUtilizing isoquinoline as a carrier ligand, we have evaluated the reactivity of selected trans‑platinum planar amine (TPA) carboxylate compounds by varying the leaving carboxylate group (acetate, hydroxyacetate, and lactate) in an effort to optimize the cytotoxic and metabolic efficiency. To measure the pharmacological properties of these compounds, a combination of systematic biophysical and biological studies were carried out mainly involving substitution reaction with NAM (N-acetyl-methionine), effects on DNA structura...
Source: Journal of Inorganic Biochemistry - January 10, 2024 Category: Biochemistry Authors: Vijay Menon Samantha J Katner Daniel E Lee Erica J Peterson Jennifer E Koblinski Nicholas P Farrell Source Type: research
Decomposition of 2,4-dihalophenols by dehaloperoxidase activity and spontaneous reaction with hydrogen peroxide
J Inorg Biochem. 2024 Mar;252:112473. doi: 10.1016/j.jinorgbio.2023.112473. Epub 2023 Dec 24.ABSTRACTThe enzyme dehaloperoxidase (DHP) found in the marine worm Amphitrite ornata is capable of enzymatic peroxidation of 2,4-dichlorophenol (DCP) and 2,4-dibromophenol (DBP). There is also at least one parallel oxidative pathway and the major products 2-chloro-1,4-benzoquinone (2-ClQ) and 2-bromo-1,4-benzoquinone (2-BrQ) undergo aspontaneous secondary hydroxylation reaction. The oxidation and hydroxylation reactions have been monitored by UV-visible spectroscopy, High Performance Liquid Chromatography (HPLC), and mass spectrome...
Source: Journal of Inorganic Biochemistry - January 10, 2024 Category: Biochemistry Authors: Mst Sharmin Aktar Ransom Hill Wyatt Holbert Stefan Franzen Source Type: research
Chronic nickel exposure alters extracellular vesicles to mediate cancer progression via sustained NUPR1 expression
This study is the first to demonstrate the role of NUPR1 in extracellular vesicle-mediate cancer progression.PMID:38199052 | DOI:10.1016/j.jinorgbio.2023.112477 (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - January 10, 2024 Category: Biochemistry Authors: Shan Liu Max Costa Angelica Ortiz Source Type: research
DNA structural changes (photo)induced by tricarbonyl (pterin)rhenium(I) complex
We report on interactions of different types of DNA molecules including double-stranded and plasmid DNA as well as polynucleotides (poly[dGdC]2 and poly[dAdT]2) with fac-[ReI(CO)3(pterin)(H2O)] (or Reptr) complex. The interaction was characterized spectroscopically and changes in the plasmid structure were verified by both electrophoresis and AFM microscopy. For comparative reasons, two others related tricarbonyl rhenium(I) complexes, fac-[(4,4'-bpy)ReI(CO)3(dppz)]+ (or Redppz) and fac-[(CF3SO3)ReI(CO)3(2,2'-bpy)] (or Rebpy) were also studied to further explore the influence of the different co-ligands on the interaction a...
Source: Journal of Inorganic Biochemistry - January 5, 2024 Category: Biochemistry Authors: F Ragone J G Ya ñuk F M Cabrerizo E Prieto E Wolcan G T Ruiz Source Type: research
Intramolecular C-H bond amination catalyzed by myoglobin reconstituted with iron porphycene
J Inorg Biochem. 2024 Mar;252:112459. doi: 10.1016/j.jinorgbio.2023.112459. Epub 2023 Dec 21.ABSTRACTC-H bond amination is an effective way to obtain nitrogen-containing products. In this work, we demonstrate that myoglobin reconstituted with iron porphycene (rMb(FePc)) catalyzes intramolecular C(sp3)-H bond amination of arylsulfonyl azides to yield corresponding sultam analogs. The total turnover number of rMb(FePc) is up to 5.7 × 104 for the C-H bond amination of 2,4,6-triisopropylbenzenesulfonyl azide. Moreover, rMb(FePc) exhibits higher selectivity for the desired C-H bond amination than the competing azide reduction ...
Source: Journal of Inorganic Biochemistry - January 5, 2024 Category: Biochemistry Authors: Yoshiyuki Kagawa Koji Oohora Takashi Hayashi Source Type: research
DNA structural changes (photo)induced by tricarbonyl (pterin)rhenium(I) complex
We report on interactions of different types of DNA molecules including double-stranded and plasmid DNA as well as polynucleotides (poly[dGdC]2 and poly[dAdT]2) with fac-[ReI(CO)3(pterin)(H2O)] (or Reptr) complex. The interaction was characterized spectroscopically and changes in the plasmid structure were verified by both electrophoresis and AFM microscopy. For comparative reasons, two others related tricarbonyl rhenium(I) complexes, fac-[(4,4'-bpy)ReI(CO)3(dppz)]+ (or Redppz) and fac-[(CF3SO3)ReI(CO)3(2,2'-bpy)] (or Rebpy) were also studied to further explore the influence of the different co-ligands on the interaction a...
Source: Journal of Inorganic Biochemistry - January 5, 2024 Category: Biochemistry Authors: F Ragone J G Ya ñuk F M Cabrerizo E Prieto E Wolcan G T Ruiz Source Type: research
Intramolecular C-H bond amination catalyzed by myoglobin reconstituted with iron porphycene
J Inorg Biochem. 2023 Dec 21;252:112459. doi: 10.1016/j.jinorgbio.2023.112459. Online ahead of print.ABSTRACTC-H bond amination is an effective way to obtain nitrogen-containing products. In this work, we demonstrate that myoglobin reconstituted with iron porphycene (rMb(FePc)) catalyzes intramolecular C(sp3)-H bond amination of arylsulfonyl azides to yield corresponding sultam analogs. The total turnover number of rMb(FePc) is up to 5.7 × 104 for the C-H bond amination of 2,4,6-triisopropylbenzenesulfonyl azide. Moreover, rMb(FePc) exhibits higher selectivity for the desired C-H bond amination than the competing azide re...
Source: Journal of Inorganic Biochemistry - January 5, 2024 Category: Biochemistry Authors: Yoshiyuki Kagawa Koji Oohora Takashi Hayashi Source Type: research
DNA structural changes (photo)induced by tricarbonyl (pterin)rhenium(I) complex
We report on interactions of different types of DNA molecules including double-stranded and plasmid DNA as well as polynucleotides (poly[dGdC]2 and poly[dAdT]2) with fac-[ReI(CO)3(pterin)(H2O)] (or Reptr) complex. The interaction was characterized spectroscopically and changes in the plasmid structure were verified by both electrophoresis and AFM microscopy. For comparative reasons, two others related tricarbonyl rhenium(I) complexes, fac-[(4,4'-bpy)ReI(CO)3(dppz)]+ (or Redppz) and fac-[(CF3SO3)ReI(CO)3(2,2'-bpy)] (or Rebpy) were also studied to further explore the influence of the different co-ligands on the interaction a...
Source: Journal of Inorganic Biochemistry - January 5, 2024 Category: Biochemistry Authors: F Ragone J G Ya ñuk F M Cabrerizo E Prieto E Wolcan G T Ruiz Source Type: research
Intramolecular C-H bond amination catalyzed by myoglobin reconstituted with iron porphycene
J Inorg Biochem. 2023 Dec 21;252:112459. doi: 10.1016/j.jinorgbio.2023.112459. Online ahead of print.ABSTRACTC-H bond amination is an effective way to obtain nitrogen-containing products. In this work, we demonstrate that myoglobin reconstituted with iron porphycene (rMb(FePc)) catalyzes intramolecular C(sp3)-H bond amination of arylsulfonyl azides to yield corresponding sultam analogs. The total turnover number of rMb(FePc) is up to 5.7 × 104 for the C-H bond amination of 2,4,6-triisopropylbenzenesulfonyl azide. Moreover, rMb(FePc) exhibits higher selectivity for the desired C-H bond amination than the competing azide re...
Source: Journal of Inorganic Biochemistry - January 5, 2024 Category: Biochemistry Authors: Yoshiyuki Kagawa Koji Oohora Takashi Hayashi Source Type: research
DNA structural changes (photo)induced by tricarbonyl (pterin)rhenium(I) complex
We report on interactions of different types of DNA molecules including double-stranded and plasmid DNA as well as polynucleotides (poly[dGdC]2 and poly[dAdT]2) with fac-[ReI(CO)3(pterin)(H2O)] (or Reptr) complex. The interaction was characterized spectroscopically and changes in the plasmid structure were verified by both electrophoresis and AFM microscopy. For comparative reasons, two others related tricarbonyl rhenium(I) complexes, fac-[(4,4'-bpy)ReI(CO)3(dppz)]+ (or Redppz) and fac-[(CF3SO3)ReI(CO)3(2,2'-bpy)] (or Rebpy) were also studied to further explore the influence of the different co-ligands on the interaction a...
Source: Journal of Inorganic Biochemistry - January 5, 2024 Category: Biochemistry Authors: F Ragone J G Ya ñuk F M Cabrerizo E Prieto E Wolcan G T Ruiz Source Type: research
Intramolecular C-H bond amination catalyzed by myoglobin reconstituted with iron porphycene
J Inorg Biochem. 2023 Dec 21;252:112459. doi: 10.1016/j.jinorgbio.2023.112459. Online ahead of print.ABSTRACTC-H bond amination is an effective way to obtain nitrogen-containing products. In this work, we demonstrate that myoglobin reconstituted with iron porphycene (rMb(FePc)) catalyzes intramolecular C(sp3)-H bond amination of arylsulfonyl azides to yield corresponding sultam analogs. The total turnover number of rMb(FePc) is up to 5.7 × 104 for the C-H bond amination of 2,4,6-triisopropylbenzenesulfonyl azide. Moreover, rMb(FePc) exhibits higher selectivity for the desired C-H bond amination than the competing azide re...
Source: Journal of Inorganic Biochemistry - January 5, 2024 Category: Biochemistry Authors: Yoshiyuki Kagawa Koji Oohora Takashi Hayashi Source Type: research
Effect of proline content and histidine ligation on the dynamics of Ω-loop D and the peroxidase activity of iso-1-cytochrome c
J Inorg Biochem. 2023 Dec 28;252:112474. doi: 10.1016/j.jinorgbio.2023.112474. Online ahead of print.ABSTRACTTo study how proline residues affect the dynamics of Ω-loop D (residues 70 to 85) of cytochrome c, we prepared G83P and G83A variants of yeast iso-1-cytochrome c (iso-1-Cytc) in the presence and absence of a K73H mutation. Ω-loop D is important in controlling both the electron transfer function of Cytc and the peroxidase activity of Cytc used in apoptosis because it provides the Met80 heme ligand. The G83P and G83A mutations have no effect on the global stability of iso-1-Cytc in presence or absence of the K73H mu...
Source: Journal of Inorganic Biochemistry - January 4, 2024 Category: Biochemistry Authors: William J Martin Levi J McClelland Shiloh M Nold Kassandra L Boshae Bruce E Bowler Source Type: research
Probing the mechanism of the dedicated NO sensor [4Fe-4S] NsrR: the effect of cluster ligand environment
We report here the mechanistic details of sequential steps of nitrosylation as observed by ESI MS and IR spectroscopy. Parallel non-denaturing mass spectrometry analyses were performed using site-differentiated variants of NsrR with the native aspartic acid, cysteine, or alanine in the position of the forth ligand to the cluster. A mono-nitrosylated synthetic [4Fe-4S] cluster was observed for the first time in a biologically-relevant thiolate-based coordination environment. Combined synthetic and protein data give unprecedented clarity in the modulation of nitrosylation of a [4Fe-4S] cluster.PMID:38176366 | DOI:10.1016/j.j...
Source: Journal of Inorganic Biochemistry - January 4, 2024 Category: Biochemistry Authors: Erin L Dodd Nick E Le Brun Source Type: research
Effect of proline content and histidine ligation on the dynamics of Ω-loop D and the peroxidase activity of iso-1-cytochrome c
J Inorg Biochem. 2023 Dec 28;252:112474. doi: 10.1016/j.jinorgbio.2023.112474. Online ahead of print.ABSTRACTTo study how proline residues affect the dynamics of Ω-loop D (residues 70 to 85) of cytochrome c, we prepared G83P and G83A variants of yeast iso-1-cytochrome c (iso-1-Cytc) in the presence and absence of a K73H mutation. Ω-loop D is important in controlling both the electron transfer function of Cytc and the peroxidase activity of Cytc used in apoptosis because it provides the Met80 heme ligand. The G83P and G83A mutations have no effect on the global stability of iso-1-Cytc in presence or absence of the K73H mu...
Source: Journal of Inorganic Biochemistry - January 4, 2024 Category: Biochemistry Authors: William J Martin Levi J McClelland Shiloh M Nold Kassandra L Boshae Bruce E Bowler Source Type: research
