Structural and functional insights into corrinoid iron-sulfur protein from human pathogen Clostridium difficile.
In this study, CoFeSP from Clostridium difficile (CoFeSPCd) was cloned, expressed in E. coli and characterized for the first time. The structure and function of CoFeSPCd were investigated using homology structure modeling, spectroscopy, electrochemistry, steady state/pre-steady state kinetics and molecular docking. The two metal centers of CoFeSPCd, corrinoid cofactor and [4Fe-4S] cluster, were characterized using metal analysis, structural modeling, UV-Vis, EPR and direct electrochemistry. The methyl transfer activity between CH3-H4folate (CH3-THF) and CoFeSPCd catalyzed by methyl transferase (MeTrCd) was determined by ki...
Source: Journal of Inorganic Biochemistry - February 13, 2017 Category: Biochemistry Authors: Wei Y, Zhu X, Zhang S, Tan X Tags: J Inorg Biochem Source Type: research

New Pt-NNSO core anticancer agents: Structural optimization and investigation of their anticancer activity.
Abstract A series of new platinum Pt(II) compounds possessing a bidentate leaving ligand modified from oxaliplatin has been synthesized, with one of the oxygen ligating atom substituted for a sulphur atom (resulting in a Pt-NNSO coordination core structure). The general structures are R,R-diaminocyclohexane (DACH)-Pt-(methylthio)acetic acid (K4) and DACH-Pt-(thiophenylacetic acid) (K4 derivatives). Substitution of an electron donating or withdrawing group at the ortho or para position on the phenyl ring of K4 derivatives was found to affect the complexes' stability, reactivity with the biological molecules (5'-gua...
Source: Journal of Inorganic Biochemistry - February 12, 2017 Category: Biochemistry Authors: Chong SX, Jin Y, Au-Yeung SC, To KK Tags: J Inorg Biochem Source Type: research

The dissociation of the Hsp60/pro-Caspase-3 complex by bis(pyridyl)oxadiazole copper complex (CubipyOXA) leads to cell death in NCI-H292 cancer cells.
Abstract Cell survival and proliferation are central to carcinogenesis, involving various mechanisms among which those that impede apoptosis are important. In this, the role of the molecular chaperone Hsp60 is unclear since it has been reported that it can be both, pro- or anti-apoptotic. A solution to this riddle is crucial to the development of anti-cancer therapies targeting Hsp60. We addressed this question using a tumor cell line, NCI-H292, and [Cu(3,5-bis(2'-pyridyl)-1,2,4-oxadiazole)2(H2O)2](ClO4)2, CubipyOXA, a copper-containing compound with cytotoxic properties. We treated cells with various doses of the...
Source: Journal of Inorganic Biochemistry - February 10, 2017 Category: Biochemistry Authors: Caruso Bavisotto C, Nikolic D, Marino Gammazza A, Barone R, Lo Cascio F, Mocciaro E, Zummo G, Conway de Macario E, Macario AJ, Cappello F, Giacalone V, Pace A, Barone G, Palumbo Piccionello A, Campanella C Tags: J Inorg Biochem Source Type: research

Interplay between autophagy and apoptosis in selenium deficient cardiomyocytes in chicken.
Abstract Dietary selenium (Se) deficiency can cause heart dysfunction, however the exact mechanism remains unclear. To understand this mechanism, 180day-old chicks, divided into two groups, C (control group) and L (low Se group), were fed with either a Se-sufficient (0.23mg/kg) or Se-deficient (0.033mg/kg) diets for 25days, respectively. Heart tissues and blood samples were collected. In L group, the activities of serum creatine kinase (CK) and creatine kinase-myoglobin (CK-MB) increased and typical ultrastructural apoptotic features were observed. Se deficiency up-regulated the mRNA levels of Cysteinyl aspartate ...
Source: Journal of Inorganic Biochemistry - February 10, 2017 Category: Biochemistry Authors: Yang J, Zhang Y, Hamid S, Cai J, Liu Q, Li H, Zhao R, Wang H, Xu S, Zhang Z Tags: J Inorg Biochem Source Type: research

Water-soluble metalloporphyrinates with excellent photo-induced anticancer activity resulting from high tumor accumulation.
Abstract To develop a water-soluble and tumor-targeted photosensitizer for photodynamic therapy (PDT), a porphyrin framework containing the metal ion gallium(III) was combined with platinum(II)-based groups to produce two new pentacationic metalloporphyrinates, Ga-4cisPtTPyP (5,10,15,20-tetrakis{cis-diammine-chloro-platinum(II)}(4-pyridyl)-porphyrinato gallium(III) hydroxide tetranitrate) and Ga-4transPtTPyP (5,10,15,20-tetrakis{trans-diammine-chloro-platinum(II)} (4-pyridyl)-porphyrinato gallium(III) hydroxide tetranitrate). Both complexes exhibited high singlet oxygen quantum yields (Φ∆) and remarkable pho...
Source: Journal of Inorganic Biochemistry - February 4, 2017 Category: Biochemistry Authors: Hu X, Ogawa K, Kiwada T, Odani A Tags: J Inorg Biochem Source Type: research

Probing nitrite coordination in horseradish peroxidase by resonance Raman spectroscopy: Detection of two binding sites.
Abstract Nitrite is a powerful oxidant that affects the activity of peroxidases towards various substrates and leads to heme macrocycle modifications in members of the peroxidase family, such as the horseradish peroxidase (HRP). We have applied resonance Raman spectroscopy to investigate the structural properties of the species formed in the reaction of NO2(-) with the ferric form of HRP. Our data demonstrate that the heme nitrovinyl group is partially formed at near neutral pH, without coordination of NO2(-) to the heme Fe. Nitrite coordinates to the heme Fe at acidic pH in the nitro binding mode, characterized b...
Source: Journal of Inorganic Biochemistry - January 25, 2017 Category: Biochemistry Authors: Ioannou A, Pinakoulaki E Tags: J Inorg Biochem Source Type: research

The Met80Ala and Tyr67His/Met80Ala mutants of human cytochrome c shed light on the reciprocal role of Met80 and Tyr67 in regulating ligand access into the heme pocket.
Abstract The spectroscopic and functional properties of the single Met80Ala and double Tyr67His/Met80Ala mutants of human cytochrome c have been investigated in their ferric and ferrous forms, and in the presence of different ligands, in order to clarify the reciprocal effect of these two residues in regulating the access of exogenous molecules into the heme pocket. In the ferric state, both mutants display an aquo high spin and a low spin species. The latter corresponds to an OH(-) ligand in Met80Ala but to a His in the double mutant. The existence of these two species is also reflected in the functional behavior...
Source: Journal of Inorganic Biochemistry - January 24, 2017 Category: Biochemistry Authors: Ciaccio C, Tognaccini L, Battista T, Cervelli M, Howes BD, Santucci R, Coletta M, Mariottini P, Smulevich G, Fiorucci L Tags: J Inorg Biochem Source Type: research

New copper(I) and heteronuclear copper(I)-ruthenium(II) complexes: Synthesis, structural characterization and cytotoxicity.
Abstract A new family of copper(I) complexes of general formula [Cu(dppe)(NN)](+) have been synthesized and fully characterized, with dppe=1.2-bis(diphenylphosphino)ethane and NN representing several bidentate heteroaromatic ligands: 2,2'-bipy=2.2'-bipyridine (1), Me2bpy=4.4'-dimethyl-2,2'-bipyridine (2), dpytz=3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine (3), dpp=2.3-bis(2-pyridyl)pyrazine (4), and the metallaligand [Ru(η(5)-C5H5)(PPh3)(dpp)](+) (5), yielding the bimetallic copper(I)-ruthenium(II) complex [Cu(dppe)(μ-dpp)Ru(η(5)-C5H5)(PPh3)](2+) (6). The single crystal structures of complexes (2) and (4) ...
Source: Journal of Inorganic Biochemistry - January 23, 2017 Category: Biochemistry Authors: Lopes J, Alves D, Morais TS, Costa PJ, Piedade MF, Marques F, Villa de Brito MJ, Helena Garcia M Tags: J Inorg Biochem Source Type: research

Heterologous expression of Halomonas halodenitrificans nitric oxide reductase and its N-terminally truncated NorC subunit in Escherichia coli.
Abstract Halomonas halodenitrificans nitric oxide reductase (NOR) is the membrane-bound heterodimer complex of NorC, which contains a low-spin heme c center, and NorB, which contains a low-spin heme b center, a high-spin heme b3 center, and a non-heme FeB center. The soluble domain of NorC, NorC* (ΔMet1-Val37) was heterologously expressed in Escherichia coli using expression plasmids harboring the truncated norC gene deleted of its 84 5'-terminal nucleotides. Analogous scission of the N-terminal helix as the membrane anchor took place when the whole norC gene was used. NorC* exhibited spectra typical of a lo...
Source: Journal of Inorganic Biochemistry - January 21, 2017 Category: Biochemistry Authors: Sakurai N, Kataoka K, Sugaya N, Shimodaira T, Iwamoto M, Shoda M, Horiuchi H, Kiyono M, Ohta Y, Triwiyono B, Seo D, Sakurai T Tags: J Inorg Biochem Source Type: research

Crystal structure, cytotoxicity and action mechanism of Zn(II)/Mn(II) complexes with isoquinoline ligands.
Abstract Four μ2-Cl bridged dinuclear metal complexes with isoquinoline ligands, (MPDQ)2Zn2Cl4 (1) (MPDQ=4.5-methylenedioxy-1-pyridinedihydroisoquinoline), (PYP)2Zn2Cl4 (2) (PYP=5-pyridin-2-yl-[1,3]dioxolo[4,5-g]isoquinoline), (MPDQ)2Mn2Cl4 (3),and (PYP)2Mn2Cl4 (4) were synthesized and characterized. All complexes exhibited strong proliferation inhibition activity against various cancer cells. The underlying molecular mechanisms through which they caused the cancer cell death were also elucidated. Induction of apoptosis in MGC-803 cells by complex 2 was evidenced by annexin V(+)/PI(-) detection and DiD/DAPI sta...
Source: Journal of Inorganic Biochemistry - January 6, 2017 Category: Biochemistry Authors: Wang FY, Xi QY, Huang KB, Tang XM, Chen ZF, Liu YC, Liang H Tags: J Inorg Biochem Source Type: research

Delineating hierarchy of selenotranscriptome expression and their response to selenium status in chicken central nervous system.
Abstract Selenium (Se) incorporated in selenoproteins as selenocysteine and supports various important cellular and organismal functions. We recently reported that chicken brain exhibited high priority for Se supply and retention under conditions of dietary Se deficiency and supernutrition Li et al. (2012) . However, the selenotranscriptome expressions and their response to Se status in chicken central nervous system (CNS) are unclear. To better understand the relationship of Se homeostasis and selenoproteins expression in chicken CNS, 1day-old HyLine White chickens were fed a low Se diet (Se-L, 0.028mg/g) supplem...
Source: Journal of Inorganic Biochemistry - January 4, 2017 Category: Biochemistry Authors: Jiang XQ, Cao CY, Li ZY, Li W, Zhang C, Lin J, Li XN, Li JL Tags: J Inorg Biochem Source Type: research

Synthesis, characterization, DNA interactions and antiproliferative activity on glioblastoma of iminopyridine platinum(II) chelate complexes.
J, Ceña V Abstract A series of iminopyridine platinum chelate compounds has been prepared and characterized by NMR spectroscopy and single-crystal X-ray diffraction. The complexes were evaluated in C6 tumoral cells as an in vitro model for glioblastoma multiforme. The DNA-binding properties of these complexes were studied by UV-Vis absorption and fluorescence spectroscopy and Density Functional Theory calculations were performed in an effort to rationalize the observed properties at the molecular level. The most promising drug candidate displayed a similar potency in inducing cell death to the clinically u...
Source: Journal of Inorganic Biochemistry - December 19, 2016 Category: Biochemistry Authors: Posadas I, Alonso-Moreno C, Bravo I, Carrillo-Hermosilla F, Garzón A, Villaseca N, López-Solera I, Albaladejo J, Ceña V Tags: J Inorg Biochem Source Type: research

Antileukemic activity of an arsenomolybdate in the human HL-60 and U937 leukemia cells.
Abstract The antileukemic activity, mechanisms and serum albumin interactions of an arsenomolybdate, K2Na[AsMo6O21(O2CCH2NH3)3]·6H2O (1), was evaluated in the human leukemia HL-60 and U937 cells. The results indicated that 1 could inhibit the proliferation of both leukemia cell lines in a dose-dependent manner with the 50% lethal concentration (IC50) value of 8.61μM for HL-60 and 14.50μM for U937 at 24h, compare to the positive controls, all-trans retinoic acid (ATRA) with IC50 value of 20.76μM and 14.85μM,and As2O3 with IC50 value of 6.40μM and 8.75μM at 24h, respectively (P
Source: Journal of Inorganic Biochemistry - December 16, 2016 Category: Biochemistry Authors: Li C, Cao H, Sun J, Tian R, Li D, Qi Y, Yang W, Li J Tags: J Inorg Biochem Source Type: research

Synthesis, photophysical properties and structures of organotin-Schiff bases utilizing aromatic amino acid from the chiral pool and evaluation of the biological perspective of a triphenyltin compound.
Abstract Five new organotin(IV) complexes of compositions [Me2SnL(1)] (1), [Me2SnL(2)]n (2), [Me2SnL(3)] (3), [Ph3SnL(1)H]n (4) and [Ph3SnL(3)H] (5) (where L(1)=(2S)-2-((E)-((Z)-4-hydroxypent-3-en-2-ylidene)amino)-3-(1H-indol-3-yl)propanoate, L(2)=(2S)-(E)-2-((2-hydroxybenzylidene)amino)-3-(1H-indol-3-yl)propanoate and L(3)=(2S)-(E)-2-((1-(2-hydroxyphenyl)ethylidene)amino)-3-(1H-indol-3-yl)propanoate were synthesized and spectroscopically characterized. The crystal structures of 1-4 were determined. For the dimethyltin derivative 2, a polymeric chain structure was observed as a result of a long Sn∙∙∙O contac...
Source: Journal of Inorganic Biochemistry - December 16, 2016 Category: Biochemistry Authors: Basu Baul TS, Kehie P, Duthie A, Guchhait N, Raviprakash N, Mokhamatam RB, Manna SK, Armata N, Scopelliti M, Wang R, Englert U Tags: J Inorg Biochem Source Type: research

An unlikely DNA cleaving agent: A photo-active trinuclear Cu(II) complex based on hexaazatriphenylene.
nt KB Abstract This paper describes the synthesis of a trinuclear Cu(II) complex (4) containing a central 1,4,5,8,9,12-hexaazatriphenylene-hexacarboxylate (hat) core (3). Low, micromolar concentrations of the negatively charged parent ligand 3 and the neutral trinuclear complex 4 were found to photocleave negatively charged pUC19 plasmid DNA with high efficiency at neutral pH (350nm, 50min, 22°C). The interactions of complex 4 with double-helical DNA were studied in detail. Scavenger and colorimetric assays pointed to the formation of Cu(I), superoxide anion radicals, hydrogen peroxide, and hydroxyl radicals d...
Source: Journal of Inorganic Biochemistry - December 15, 2016 Category: Biochemistry Authors: Williams DE, Fischer CM, Kassai M, Gude L, Fernández MJ, Lorente A, Grant KB Tags: J Inorg Biochem Source Type: research

New silver complexes with bioactive glycine and nicotinamide molecules - Characterization, DNA binding, antimicrobial and anticancer evaluation.
This study introduces a pair of newly synthesized silver complexes, [Ag2(HGly)2]n(NO3)2n (1) and [Ag(Nam)2]NO3·H2O (2) (Gly - glycine, Nam - nicotinamide), that were prepared and characterized by relevant methods in solid state (elemental, spectral, thermal and structural analysis) and their stability in solution was verified by (1)H NMR measurements. Moreover, suitable reaction conditions were observed by potentiometry depending on pH in case of binary system Ag-Gly. X-ray analysis confirmed argentophilic interactions in complex 1 with an Ag1-Ag2 distance of 2.8018(6) Å. Antimicrobial testing indicates higher...
Source: Journal of Inorganic Biochemistry - December 15, 2016 Category: Biochemistry Authors: Rendošová M, Vargová Z, Kuchár J, Sabolová D, Levoča Š, Kudláčová J, Paulíková H, Hudecová D, Helebrandtová V, Almáši M, Vilková M, Dušek M, Bobáľová D Tags: J Inorg Biochem Source Type: research

Silver complexes of ligands derived from adamantylamines: Water-soluble silver-donating compounds with antibacterial properties.
Abstract Two new silver(I) complexes, namely [Ag(qyAm)2](CF3SO3) (1) and [Ag(qyTAm)2](CF3SO3) (2), (qyAm=2-(quinonyl)iminoadamantane, qyTAm=2-(quinonyl)iminotriazaadamantane) have been synthesized and characterized by elemental analyses, (1)H NMR, IR, electronic absorption spectroscopy, and X-ray diffraction. The coordination geometry of the silver center in both complexes is distorted tetrahedral where their respective qyAm and qyTAm ligand bind in a bidentate fashion using the imine and quinoline nitrogen atoms. Complex 2 is soluble in water and exhibits strong antimicrobial actions on both Gram-negative (E. col...
Source: Journal of Inorganic Biochemistry - December 10, 2016 Category: Biochemistry Authors: Jimenez J, Chakraborty I, Rojas-Andrade M, Mascharak PK Tags: J Inorg Biochem Source Type: research

Sequestering ability to Cu(2+) of a new bodipy-based dye and its behavior as in vitro fluorescent sensor.
oti C Abstract A Bodipy (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) derivative has been conceived and synthesized starting from l-aspartic acid, as a selective turn-off sensor of Cu(2+) ions. Its acid-base properties were determined to study the formation of metal/sensor complex species by titration of solutions each containing a different metal ion, such as Cu(2+), Ca(2+), Zn(2+), Pb(2+) and Hg(2+) and different metal/sensor ratios. The speciation models allowed us to simulate the distribution of the metal/sensor complex species at the normal concentrations of the corresponding metals present in biological fluid...
Source: Journal of Inorganic Biochemistry - December 2, 2016 Category: Biochemistry Authors: Papalia T, Barattucci A, Barreca D, Bellocco E, Bonaccorsi P, Minuti L, Nicolò MS, Temperini A, Foti C Tags: J Inorg Biochem Source Type: research

Preface.
PMID: 27938735 [PubMed - in process] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - December 1, 2016 Category: Biochemistry Authors: Casini A, Contel M Tags: J Inorg Biochem Source Type: research

Challenges in assignment of allosteric effects in cytochrome P450-catalyzed substrate oxidations to structural dynamics in the hemoprotein architecture.
Abstract Cytochrome P450s (CYP) represent a superfamily of b-type hemoproteins catalyzing NAD(P)H-dependent oxidative biotransformation of a vast array of natural and xenobiotic compounds. Many eu- and prokaryotic members of this class of monooxygenases display complex non-Michaelis-Menten saturation kinetics, suggestive of homo-/heterotropic cooperativity arising from substrate-/effector-induced allosteric interactions. Here, the paradigm of multiple-ligand occupancy of the catalytic pocket in combination with enzyme oligomerization provides the most favored explanations for the atypical kinetic patterns. Making ...
Source: Journal of Inorganic Biochemistry - November 25, 2016 Category: Biochemistry Authors: Hlavica P Tags: J Inorg Biochem Source Type: research

Biophysical characterization and antineoplastic activity of new bis(thiosemicarbazonato) Cu(II) complexes.
Abstract Aiming to explore alternative mechanisms of cellular uptake and cytotoxicity, we have studied a new family of copper(II) complexes (CuL(1)-CuL(4)) with bis(thiosemicarbazone) (BTSC) ligands containing pendant protonable cyclic amines (morpholine and piperidine). Herein, we report on the synthesis and characterization of these new complexes, as well as on their biological performance (cytotoxic activity, cellular uptake, protein and DNA binding), in comparison with the parental Cu(II)ATSM (ATSM=diacetyl-bis(N4-methylthiosemicarbazonate) complex without pendant cyclic amines. The new compounds have been cha...
Source: Journal of Inorganic Biochemistry - November 23, 2016 Category: Biochemistry Authors: Palma E, Mendes F, Morais GR, Rodrigues I, Santos IC, Campello MP, Raposinho P, Correia I, Gama S, Belo D, Alves V, Abrunhosa AJ, Santos I, Paulo A Tags: J Inorg Biochem Source Type: research

Drug discovery targeting heme-based sensors and their coupled activities.
Abstract Heme-based sensors have emerged during the last 20years as being a large family of proteins that occur in all kingdoms of life. A myriad of biological adaptations are associated with these sensors, which include vasodilation, bacterial virulence, dormancy, chemotaxis, biofilm formation, among others. Due to the key activities regulated by these proteins along with many other systems that use similar output domains, there is a growing interest in developing small molecules as their regulators. Here, we review the development of potential activators and inhibitors for many of these systems, including human ...
Source: Journal of Inorganic Biochemistry - November 20, 2016 Category: Biochemistry Authors: Sousa EH, Lopes LG, Gonzalez G, Gilles-Gonzalez MA Tags: J Inorg Biochem Source Type: research

Ferrocenyl naphthalene diimides as tetraplex DNA binders.
Abstract Seven ferrocenyl naphthalene diimide (FND) ligands were synthesized. Each had a higher affinity for tetraplex DNA than for either single- or double-stranded DNA. The FND binding affinities were>10(5)M(-1) in 0.10M AcOH-AcONa or AcOH-AcOK (pH5.5) containing 0.10M NaCl or KCl. The FNDs with the highest binding affinities for tetraplex DNA showed 23- or 8-times higher preference for tetraplex DNA than for single- or double-stranded DNA, respectively. The current signals generated from the seven FNDs bound to the tetraplex DNA immobilized on the electrode were found to correlate with the binding affinities...
Source: Journal of Inorganic Biochemistry - November 17, 2016 Category: Biochemistry Authors: Sato S, Takenaka S Tags: J Inorg Biochem Source Type: research

May glutamine addiction drive the delivery of antitumor cisplatin-based Pt(IV) prodrugs?
Abstract A small series of Pt(IV) prodrugs containing Gln-like (Gln=glutamine) axial ligands has been designed with the aim to take advantage of the increased demand of Gln showed by some cancer cells (glutamine addiction). In complex 4 the Gln, linked through the α-carboxylic group is recognized by the Gln transporters, in particular by the solute carrier transporter SLC1A5. All compounds showed cellular accumulation, as well as antiproliferative activity, related to their lipophilicity, as already demonstrated for the majority of Pt(IV) prodrugs, that enter cells mainly by passive diffusion. On the contrar...
Source: Journal of Inorganic Biochemistry - November 17, 2016 Category: Biochemistry Authors: Ravera M, Gabano E, Tinello S, Zanellato I, Osella D Tags: J Inorg Biochem Source Type: research

Quercetin loading CdSe/ZnS nanoparticles as efficient antibacterial and anticancer materials.
In this study, we described a simple method for synthesis of Qe/CdSe/ZnS nanoparticles (QCZ NPs). The QCZ NPs had an average diameter of 10nm and prominent yellow emission under UV irradiation. We investigated the antibacterial activity of QCZ NPs against drug-resistant Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis) in vitro. Results showed that QCZ NPs had considerably more effective antibacterial activities than Qe or CdSe nanoparticles (CdSe NPs). Antibacterial experiment results showed that QCZ NPs acted against E. coli and B. subtilis by disrupting the bacterial cell wall and membrane. In vivo study, t...
Source: Journal of Inorganic Biochemistry - November 17, 2016 Category: Biochemistry Authors: Yang X, Zhang W, Zhao Z, Li N, Mou Z, Sun D, Cai Y, Wang W, Lin Y Tags: J Inorg Biochem Source Type: research

Ru(III) anticancer agents with aromatic and non-aromatic dithiocarbamates as ligands: Loading into nanocarriers and preliminary biological studies.
Abstract Since the discovery of cisplatin in the 1960s, other metal complexes have been investigated as potential antitumor agents to overcome the side-effects associated with the administration of the Pt-based drug. In line with our previous research, in this work we report the synthesis and characterization of mono- and dinuclear Ru(III) complexes with the pyrrolidinedithiocarbamate (PDT) ligand and the more sterically-hindered carbazole-dithiocarbamato ligand (CDT), to compare their properties (both at the chemical and antiproliferative level), in an attempt to assess a structure-activity rationale. Moreover, t...
Source: Journal of Inorganic Biochemistry - November 16, 2016 Category: Biochemistry Authors: Scintilla S, Brustolin L, Gambalunga A, Chiara F, Trevisan A, Nardon C, Fregona D Tags: J Inorg Biochem Source Type: research

Metagenomics analysis reveals a new metallothionein family: Sequence and metal-binding features of new environmental cysteine-rich proteins.
het L Abstract Metallothioneins are cysteine-rich proteins, which function as (i) metal carriers in basal cell metabolism and (ii) protective metal chelators in conditions of metal excess. Metallothioneins have been characterized from different eukaryotic model and cultivable species. Presently, they are categorized in 15 families but evolutionary relationships between these metallothionein families remain unresolved. Several cysteine-rich protein encoding genes that conferred Cd-tolerance in Cd-sensitive yeast mutants have previously been isolated from soil eukaryotic metatranscriptomes. They were called CRPs for...
Source: Journal of Inorganic Biochemistry - November 12, 2016 Category: Biochemistry Authors: Ziller A, Yadav RK, Capdevila M, Reddy MS, Vallon L, Marmeisse R, Atrian S, Palacios Ò, Fraissinet-Tachet L Tags: J Inorg Biochem Source Type: research

Inactivation of urease by catechol: Kinetics and structure.
In this study, we describe a molecular characterization of the interaction between urease from Sporosarcina pasteurii (SPU) and Canavalia ensiformis (jack bean, JBU) with catechol, a model polyphenol. In particular, catechol irreversibly inactivates both SPU and JBU with a complex radical-based autocatalytic multistep mechanism. The crystal structure of the SPU-catechol complex, determined at 1.50Å resolution, reveals the structural details of the enzyme inhibition. PMID: 27888701 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - November 9, 2016 Category: Biochemistry Authors: Mazzei L, Cianci M, Musiani F, Lente G, Palombo M, Ciurli S Tags: J Inorg Biochem Source Type: research

Evaluation of cellular uptake, cytotoxicity and cellular ultrastructural effects of heteroleptic oxidovanadium(IV) complexes of salicylaldimines and polypyridyl ligands.
, Gambino D Abstract Searching for prospective vanadium-based drugs for cancer treatment, a new series of structurally related [V(IV)O(L-2H)(NN)] compounds (1-8) was developed. They include a double deprotonated salicylaldimine Schiff base ligand (L-2H) and different NN-polypyridyl co-ligands having DNA intercalating capacity. Compounds were characterized in solid state and in solution. EPR spectroscopy suggests that the NN ligands act as bidentate and bind through both nitrogen donor atoms in an axial-equatorial mode. The cytotoxicity was evaluated in human tumoral cells (ovarian A2780, breast MCF7, prostate PC3)...
Source: Journal of Inorganic Biochemistry - November 9, 2016 Category: Biochemistry Authors: Scalese G, Correia I, Benítez J, Rostán S, Marques F, Mendes F, Matos AP, Costa Pessoa J, Gambino D Tags: J Inorg Biochem Source Type: research

Mammalian agmatinases constitute unusual members in the family of Mn(2+)-dependent ureahydrolases.
G, Carvajal N, Uribe E Abstract Agmatine (1-amino-4-guanidinobutane) plays an important role in a range of metabolic functions, in particular in the brain. Agmatinases (AGMs) are enzymes capable of converting agmatine to the polyamine putrescine and urea. AGMs belong to the family of Mn(2+)-dependent ureahydrolases. However, no AGM from a mammalian source has yet been extracted in catalytically active form. While in human AGM the six amino acid ligands that coordinate the two Mn(2+) ions in the active site are conserved, four mutations are observed in the murine enzyme. Here, we demonstrate that similar to its hu...
Source: Journal of Inorganic Biochemistry - November 9, 2016 Category: Biochemistry Authors: Romero N, Benítez J, Garcia D, González A, Bennun L, García-Robles MA, López V, Wilson LA, Schenk G, Carvajal N, Uribe E Tags: J Inorg Biochem Source Type: research

Targeting human telomeric and c-myc G-quadruplexes with alkynylplatinum(II) terpyridine complexes under molecular crowding conditions.
Abstract The interactions between alkynylplatinum(II) terpyridine complexes 1-3 and the G-quadruplex DNA, including c-myc and telomeric quadruplex DNA, are investigated both in dilute solution and under molecular crowding conditions. The UV-vis absorption spectroscopy, circular dichroism and molecular docking studies suggest that 1-3 associate with telomeric and c-myc G-quadruplexes via groove binding, and electrostatic interactions. Experimental studies indicate that under molecular crowding conditions (in the presence of 40wt% PEG 200), 1-2 show weak affinity for c-myc, while 3 still displays high affinity and s...
Source: Journal of Inorganic Biochemistry - November 9, 2016 Category: Biochemistry Authors: Ou Z, Wang Y, Gao Y, Wang X, Qian Y, Li Y, Wang X Tags: J Inorg Biochem Source Type: research

Aza-bridged bisphenanthrolinyl Pt(II) complexes: Efficient stabilization and topological selectivity on telomeric G-quadruplexes.
This study paved the way to develop Pt complexes as GQ stabilizers for specific folding topology and the applications to disease and/or personalized anticancer medicine/therapy. PMID: 27852005 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - November 9, 2016 Category: Biochemistry Authors: He L, Meng Z, Xie YQ, Chen X, Li T, Shao F Tags: J Inorg Biochem Source Type: research

Nanosized mesoporous metal-organic framework MIL-101 as a nanocarrier for photoactive hexamolybdenum cluster compounds.
Abstract Inclusion compounds of photoluminescent hexamolybdenum cluster complexes in the chromium terephthalate metal-organic framework, MIL-101 (MIL, Matérial Institut Lavoisier) were successfully synthesized in two different ways and characterized by means of powder X-Ray diffraction, chemical analysis and nitrogen sorption. Some important functional properties of hexamolybdenum cluster complexes for biological and medical applications, in particular singlet oxygen generation ability, luminescence properties, cellular uptake behavior and cytotoxicity were studied. It was revealed that the inclusion compou...
Source: Journal of Inorganic Biochemistry - November 9, 2016 Category: Biochemistry Authors: Cheplakova AM, Solovieva AO, Pozmogova TN, Vorotnikov YA, Brylev KA, Vorotnikova NA, Vorontsova EV, Mironov YV, Poveshchenko AF, Kovalenko KA, Shestopalov MA Tags: J Inorg Biochem Source Type: research

Inorganic mesoporous silicas as vehicles of two novel anthracene-based ruthenium metalloarenes.
Abstract Two novel anthracene-based half-sandwich organometallic Ru(II) compounds, namely, [Ru(p-cymene)(L1)Cl2] (1) and [Ru(p-cymene)(L2)Cl2] (2) (L1=1-(anthracen-9-yl)-N-(pyridin-3-ylmethyl)methanamine; L2=1-(anthracen-9-yl)-N-(pyridin-4-ylmethyl)methanamine) have been synthesized and characterized. We demonstrate that the fluorescence properties of these complexes are highly affected by the linking position of the anthracene unit, as only 2 shows fluorescence emission in the blue region. Regarding their biological activity, both ruthenium metallodrugs show interaction with different biological targets such as S...
Source: Journal of Inorganic Biochemistry - November 7, 2016 Category: Biochemistry Authors: Rojas S, Carmona FJ, Barea E, Maldonado CR Tags: J Inorg Biochem Source Type: research

Genotoxic assessment of the copper chelated compounds Casiopeinas: Clues about their mechanisms of action.
Abstract Casiopeinas is the generic name of a group of copper chelated complexes designed to be used as antineoplastic. Some of these compounds have shown promising results, and in fact, one of them named Casiopeina III-ia has completed preclinical trials and is ready to start clinical phase I in Mexico. As part of the tests that have to be done to every molecule intended to be used in humans, bacterial assays are required because of their sensitivity, speed and reproducibility and among them, Ames test and the SOS Chromotest are widely used to evaluate DNA damage. With the aim to contribute to complete safety inf...
Source: Journal of Inorganic Biochemistry - November 5, 2016 Category: Biochemistry Authors: Serment-Guerrero J, Bravo-Gomez ME, Lara-Rivera E, Ruiz-Azuara L Tags: J Inorg Biochem Source Type: research

Synthesis and antibacterial activity of silver@carbon nanocomposites.
Abstract In this work, hollow multiple-Ag-nanoclustes- C-shell nanocomposites (Ag@C) were synthesized by using silane coupling agent to graft carbon dots (CDs) with silver nanoparticles (AgNPs). CDs act as coating and stabilizing agent, protecting AgNPs from aggregation and oxidation. The resulting Ag@C nanocomposites demonstrate strong bactericidal effect against both gram-negative and gram-positive bacteria in the disk diffusion test. Cellular toxicity evaluation was performed using MTT assay. Meanwhile, the as-prepared Ag@C nanocomposites show a good biocompatibility. PMID: 27835776 [PubMed - as supplied b...
Source: Journal of Inorganic Biochemistry - November 5, 2016 Category: Biochemistry Authors: Wang K, Ji Q, Li H, Guan F, Zhang D, Feng H, Fan H Tags: J Inorg Biochem Source Type: research

Synthesis of Ag(I) camphor sulphonylimine complexes and assessment of their cytotoxic properties against cisplatin-resistant A2780cisR and A2780 cell lines.
ho MF Abstract Camphorsulphonylimine complexes [Ag(NO3)((I)L)2] ((I)L=C12H19N3SO2, 1) and [(AgNO3)2((II)L)] ((II)L=C22H23N3SO2, 2) were synthesized and characterized by elemental analysis, spectroscopy (IR, NMR) and cyclic voltammetry. [Ag(NO3)((I)L)2] crystalizes in the monoclinic C2 space group with a triangular geometry assuming a chalice-type shape. The anti-proliferative properties of the new complexes 1 and 2 and those of the previously reported [Ag(NO3)((III)L)] ((III)L=C16H18N3SO2, 3) were assessed against the human ovarian cancer cells (cisplatin-sensitive A2780, cisplatin-resistant A2780cisR) and the non...
Source: Journal of Inorganic Biochemistry - November 4, 2016 Category: Biochemistry Authors: Cardoso JM, Correia I, Galvão AM, Marques F, Carvalho MF Tags: J Inorg Biochem Source Type: research

Copper (II) complexes possessing alkyl-substituted polypyridyl ligands: Structural characterization and in vitro antitumor activity.
Abstract In an effort to find alternatives to the antitumor drug cisplatin, a series of copper (II) complexes possessing alkyl-substituted polypyridyl ligands have been synthesized. Eight new complexes are reported herein: μ-dichloro-bis{2,9-di-sec-butyl-1,10-phenanthrolinechlorocopper(II)} {[((di-sec-butyl)phen)ClCu(μ-Cl)2CuCl((di-sec-butyl)phen)]}(1), 2-sec-butyl-1,10-phenanthrolinedichlorocopper(II) {([mono-sec-butyl)phen) CuCl2} (2), 2,9-di-n-butyl-1,10-phenanthrolinedichlorocopper(II) {[(di-n-butyl)phen) CuCl2}(3), 2-n-butyl-1,10-phenanthrolinedichlorocopper(II) {[(mono-n-butyl)phen) CuCl2} (4), 2,9-di-...
Source: Journal of Inorganic Biochemistry - October 22, 2016 Category: Biochemistry Authors: Angel NR, Khatib RM, Jenkins J, Smith M, Rubalcava JM, Le BK, Lussier D, Chen ZG, Tham FS, Wilson EH, Eichler JF Tags: J Inorg Biochem Source Type: research

The facial triad in the α-ketoglutarate dependent oxygenase FIH: A role for sterics in linking substrate binding to O2 activation.
The facial triad in the α-ketoglutarate dependent oxygenase FIH: A role for sterics in linking substrate binding to O2 activation. J Inorg Biochem. 2016 Oct 17;166:26-33 Authors: Hangasky JA, Taabazuing CY, Martin CB, Eron SJ, Knapp MJ Abstract The factor inhibiting hypoxia inducible factor-1α (FIH) is a nonheme Fe(II)/αKG oxygenase using a 2-His-1-Asp facial triad. FIH activates O2 via oxidative decarboxylation of α-ketoglutarate (αKG) to generate an enzyme-based oxidant which hydroxylates the Asn(803) residue within the C-terminal transactivation domain (CTAD) of HIF-1&...
Source: Journal of Inorganic Biochemistry - October 17, 2016 Category: Biochemistry Authors: Hangasky JA, Taabazuing CY, Martin CB, Eron SJ, Knapp MJ Tags: J Inorg Biochem Source Type: research

New dibutyltin(IV) ladders: Syntheses, structures and, optimization and evaluation of cytotoxic potential employing A375 (melanoma) and HCT116 (colon carcinoma) cell lines in vitro.
Abstract Synthesis and spectroscopic properties of seven new dibutyltin(IV) compounds of 2-{(E)-4-hydroxy-3-[(E)-4-(aryl)iminomethyl]phenyldiazenyl}benzoic acids (L(n)HH'; n=2-8) with general formula {[Bu2Sn(L(n)H)]2O}2 (1-7) are reported. The compounds were characterized by elemental analysis and by UV-Visible, fluorescence, IR, (1)H, (13)C and (119)Sn NMR spectroscopies. Solid state structures of dibutyltin(IV) compounds 1-3, 6 and 7 were accomplished from single crystal X-ray crystallography which reveal the common ladder-type structure with two endo- and two exo-Sn atoms. The redox properties of L(n)HH' (n=2-4...
Source: Journal of Inorganic Biochemistry - October 15, 2016 Category: Biochemistry Authors: Basu Baul TS, Dutta D, Duthie A, Guchhait N, Rocha BG, Guedes da Silva MF, Mokhamatam RB, Raviprakash N, Manna SK Tags: J Inorg Biochem Source Type: research

Nitrite coordination in myoglobin.
Abstract The coordination of nitrite in myoglobin (Mb) has been characterized by resonance Raman spectroscopy and the frequencies of the nitrite bound to the heme Fe as well to the 2-vinyl have been computed by density functional theory (DFT) calculations. The DFT Natural Bond Orbital (NBO) analysis and the extensive isotope-labeling in the resonance Raman experiments indicate that NO2(-) (O1NO2) is bound to the heme Fe via O1. Based on the vibrational characterization of the reversible transition between low and high spin FeONO/2-nitrovinyl species, we suggest that the key step that triggers the spin-change is th...
Source: Journal of Inorganic Biochemistry - October 14, 2016 Category: Biochemistry Authors: Ioannou A, Lambrou A, Daskalakis V, Pinakoulaki E Tags: J Inorg Biochem Source Type: research

Rat liver mitochondrial dysfunction by addition of copper(II) or iron(III) ions.
Repetto MG Abstract Increased copper (Cu) and iron (Fe) levels in liver and brain are associated to oxidative stress and damage with increased phospholipid oxidation process. The aim of this work was to assess the toxic effects of Cu(2+) and Fe(3+) addition to rat liver mitochondria by determining mitochondrial respiration in states 3 (active respiration) and 4 (resting respiration), and phospholipid peroxidation. Both, Cu(2+) and Fe(3+) produced decreases in O2 consumption in a concentration-dependent manner in active state 3: both ions by 42% with malate-glutamate as complex I substrate (concentration for half ...
Source: Journal of Inorganic Biochemistry - October 14, 2016 Category: Biochemistry Authors: Saporito-Magriñá C, Musacco-Sebio R, Acosta JM, Bajicoff S, Paredes-Fleitas P, Boveris A, Repetto MG Tags: J Inorg Biochem Source Type: research

Synthesis, crystal structures, DFT studies, molecular docking and urease inhibition studies of three Ni(II) complexes with a sexidentate N2O4-donor bis-Schiff base ligand.
Abstract Three Ni(II) complexes with a sexidentate N2O4-donor bis-Schiff base ligand, namely Ni(C36H34N2O8)·2CH3OH (1), Ni(C28H34N2O8S2)·H2O (2) and Ni(C40H36N4O8)·3CH3OH (3) (C36H34N2O8=1,2-bis(2-methoxy-6-formylphenoxy)ethane-l-phenylalanine; C28H34N2O8S2=1,2-bis(2-methoxy-6-formylphenoxy)ethane-l-methionine; C40H36N4O8=1,2-bis(2-methoxy-6-formylphenoxy)ethane-l-tryptophan) were synthesized and structurally characterized. Theoretical studies of the three complexes were carried out by density functional theory (DFT) Becke's three-parameter hybrid (B3LYP) method employing the 6-31G basis set. ...
Source: Journal of Inorganic Biochemistry - October 14, 2016 Category: Biochemistry Authors: Wang H, Lan T, Zhang X, Zhang D, Bi C, Fan Y Tags: J Inorg Biochem Source Type: research

Biochemical characterization and zinc binding group (ZBGs) inhibition studies on the catalytic domain of MMP7 (cdMMP7).
This study describes a well-characterized analog of MMP7 that is available for future inhibitor design efforts. PMID: 27755977 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - October 14, 2016 Category: Biochemistry Authors: Meng F, Yang H, Jack C, Zhang H, Moller A, Spivey D, Page RC, Tierney DL, Crowder MW Tags: J Inorg Biochem Source Type: research

Selective speciation improves efficacy and lowers toxicity of platinum anticancer and vanadium antidiabetic drugs.
Abstract Improving efficacy and lowering resistance to metal-based drugs can be addressed by consideration of the coordination complex speciation and key reactions important to vanadium antidiabetic drugs or platinum anticancer drugs under biological conditions. The methods of analyses vary depending on the specific metal ion chemistry. The vanadium compounds interconvert readily, whereas the reactions of the platinum compounds are much slower and thus much easier to study. However, the vanadium species are readily differentiated due to vanadium complexes differing in color. For both vanadium and platinum systems,...
Source: Journal of Inorganic Biochemistry - October 3, 2016 Category: Biochemistry Authors: Doucette KA, Hassell KN, Crans DC Tags: J Inorg Biochem Source Type: research

Antiproliferative properties and biomolecular interactions of three Pd(II) and Pt(II) complexes.
Abstract Three Pd(II) and Pt(II) complexes with chelating mono(imidazolin-2-imine) and bis(imidazolin-2-imine) ligands i.e. [Pd(DMEAIm(iPr))Cl2] (1) (DMEAIm(iPr), 2-(1,3-diisopropyl-4,5-dimethylimidazolin-2-imine)ethan-1-dimethylamine), [Pd(DACH(Im(iPr))2)Cl2] (2) (DACH(Im(iPr))2, N,N'-(cyclohexane-1,2-diyl)bis(1,3-diisopropyl-4,5-dimethylimidazolin-2-imine)) and [Pt(DMEAIm(iPr))Cl2] (3), are evaluated here as potential cytotoxic and anticancer agents. An acceptable solution behaviour was found for the three study compounds in terms of solubility and stability. Notably, the three metal complexes demonstrated moder...
Source: Journal of Inorganic Biochemistry - September 30, 2016 Category: Biochemistry Authors: Massai L, Pratesi A, Bogojeski J, Banchini M, Pillozzi S, Messori L, Bugarčić ŽD Tags: J Inorg Biochem Source Type: research

Copper (II) and zinc (II) complexes with flavanone derivatives: Identification of potential cholinesterase inhibitors by on-flow assays.
Abstract Metal chelates strongly influence the nature and magnitude of pharmacological activities in flavonoids. In recent years, studies have shown that a promising class of flavanone-metal ion complexes can act as selective cholinesterase inhibitors (ChEIs), which has led our group to synthesize a new series of flavanone derivatives (hesperidin, hesperetin, naringin, and naringenin) complexed to either copper (II) or zinc (II) and to evaluate their potential use as selective ChEIs. Most of the synthesized complexes exhibited greater inhibitory activity against acetylcholinesterase (AChE) than against butyrylchol...
Source: Journal of Inorganic Biochemistry - September 17, 2016 Category: Biochemistry Authors: Sarria AL, Vilela AF, Frugeri BM, Fernandes JB, Carlos RM, da Silva MF, Cass QB, Cardoso CL Tags: J Inorg Biochem Source Type: research

Role of subcellular calcium redistribution in regulating apoptosis and autophagy in cadmium-exposed primary rat proximal tubular cells.
Abstract Ca(2+) signaling plays a vital role in regulating apoptosis and autophagy. We previously proved that cytosolic Ca(2+) overload is involved in cadmium (Cd)-induced apoptosis in rat proximal tubular (rPT) cells, but the source of elevated cytosolic Ca(2+) concentration ([Ca(2+)]c) and the effect of potential subcellular Ca(2+) redistribution on apoptosis and autophagy remain to be elucidated. Firstly, data showed that Cd-induced elevation of [Ca(2+)]c was primarily generated intracellularly. Moreover, elevations of [Ca(2+)]c and mitochondrial Ca(2+) concentration ([Ca(2+)]mit) with depletion of endoplasmic ...
Source: Journal of Inorganic Biochemistry - September 14, 2016 Category: Biochemistry Authors: Liu F, Li ZF, Wang ZY, Wang L Tags: J Inorg Biochem Source Type: research

Effects of metal ions and cosolutes on G-quadruplex topology.
oto N Abstract Topologies of G-quadruplexes depend on oligonucleotide sequences and on environmental factors, and the diversity of G-quadruplex topologies complicates investigation of functions of these nucleic acid structures. To investigate how metal ions and cosolutes regulate topologies of G-quadruplexes, we stabilized the antiparallel conformation by insertion of 2'-deoxyxanthosine and 8-oxo-2'-deoxyguanosine into selected positions of an oligonucleotide. Thermodynamic analyses of the oligonucleotide revealed that Na(+) stabilized the antiparallel G-quadruplex, whereas K(+) destabilized this topology. This re...
Source: Journal of Inorganic Biochemistry - September 14, 2016 Category: Biochemistry Authors: Fujii T, Podbevšek P, Plavec J, Sugimoto N Tags: J Inorg Biochem Source Type: research

Chemical properties and biotoxicity of several chromium picolinate derivatives.
Abstract As a man-made additive, chromium picolinate Cr(pic)3 has become a popular dietary supplement worldwide. In this paper Cr(pic)3 and its new derivatives Cr(6-CH3-pic)3 (1), [Cr(6-NH2-pic)2(H2O)2]NO3 (2) and Cr(3-NH2-pic)3 (3) were synthesized, and complexes 1 and 2 were characterized by X-ray crystal structure (where pic=2-carboxypyridine). The relationship between the chemical properties and biotoxicity of these complexes was fully discussed: (1) The dynamics stability of chromium picolinate complexes mainly depends on the CrN bonds length. (2) There is a positive correlation between the dynamics stability...
Source: Journal of Inorganic Biochemistry - September 14, 2016 Category: Biochemistry Authors: Liu B, Liu Y, Chai J, Hu X, Wu D, Yang B Tags: J Inorg Biochem Source Type: research