Genotoxic assessment of the copper chelated compounds Casiopeinas: Clues about their mechanisms of action.
Abstract Casiopeinas is the generic name of a group of copper chelated complexes designed to be used as antineoplastic. Some of these compounds have shown promising results, and in fact, one of them named Casiopeina III-ia has completed preclinical trials and is ready to start clinical phase I in Mexico. As part of the tests that have to be done to every molecule intended to be used in humans, bacterial assays are required because of their sensitivity, speed and reproducibility and among them, Ames test and the SOS Chromotest are widely used to evaluate DNA damage. With the aim to contribute to complete safety inf...
Source: Journal of Inorganic Biochemistry - November 4, 2016 Category: Biochemistry Authors: Serment-Guerrero J, Bravo-Gomez ME, Lara-Rivera E, Ruiz-Azuara L Tags: J Inorg Biochem Source Type: research

Synthesis and antibacterial activity of silver@carbon nanocomposites.
Abstract In this work, hollow multiple-Ag-nanoclustes- C-shell nanocomposites (Ag@C) were synthesized by using silane coupling agent to graft carbon dots (CDs) with silver nanoparticles (AgNPs). CDs act as coating and stabilizing agent, protecting AgNPs from aggregation and oxidation. The resulting Ag@C nanocomposites demonstrate strong bactericidal effect against both gram-negative and gram-positive bacteria in the disk diffusion test. Cellular toxicity evaluation was performed using MTT assay. Meanwhile, the as-prepared Ag@C nanocomposites show a good biocompatibility. PMID: 27835776 [PubMed - as supplied b...
Source: Journal of Inorganic Biochemistry - November 4, 2016 Category: Biochemistry Authors: Wang K, Ji Q, Li H, Guan F, Zhang D, Feng H, Fan H Tags: J Inorg Biochem Source Type: research

Synthesis of Ag(I) camphor sulphonylimine complexes and assessment of their cytotoxic properties against cisplatin-resistant A2780cisR and A2780 cell lines.
ho MF Abstract Camphorsulphonylimine complexes [Ag(NO3)((I)L)2] ((I)L=C12H19N3SO2, 1) and [(AgNO3)2((II)L)] ((II)L=C22H23N3SO2, 2) were synthesized and characterized by elemental analysis, spectroscopy (IR, NMR) and cyclic voltammetry. [Ag(NO3)((I)L)2] crystalizes in the monoclinic C2 space group with a triangular geometry assuming a chalice-type shape. The anti-proliferative properties of the new complexes 1 and 2 and those of the previously reported [Ag(NO3)((III)L)] ((III)L=C16H18N3SO2, 3) were assessed against the human ovarian cancer cells (cisplatin-sensitive A2780, cisplatin-resistant A2780cisR) and the non...
Source: Journal of Inorganic Biochemistry - November 3, 2016 Category: Biochemistry Authors: Cardoso JM, Correia I, Galvão AM, Marques F, Carvalho MF Tags: J Inorg Biochem Source Type: research

Copper (II) complexes possessing alkyl-substituted polypyridyl ligands: Structural characterization and in vitro antitumor activity.
Abstract In an effort to find alternatives to the antitumor drug cisplatin, a series of copper (II) complexes possessing alkyl-substituted polypyridyl ligands have been synthesized. Eight new complexes are reported herein: μ-dichloro-bis{2,9-di-sec-butyl-1,10-phenanthrolinechlorocopper(II)} {[((di-sec-butyl)phen)ClCu(μ-Cl)2CuCl((di-sec-butyl)phen)]}(1), 2-sec-butyl-1,10-phenanthrolinedichlorocopper(II) {([mono-sec-butyl)phen) CuCl2} (2), 2,9-di-n-butyl-1,10-phenanthrolinedichlorocopper(II) {[(di-n-butyl)phen) CuCl2}(3), 2-n-butyl-1,10-phenanthrolinedichlorocopper(II) {[(mono-n-butyl)phen) CuCl2} (4), 2,9-di-...
Source: Journal of Inorganic Biochemistry - October 21, 2016 Category: Biochemistry Authors: Angel NR, Khatib RM, Jenkins J, Smith M, Rubalcava JM, Le BK, Lussier D, Chen ZG, Tham FS, Wilson EH, Eichler JF Tags: J Inorg Biochem Source Type: research

The facial triad in the α-ketoglutarate dependent oxygenase FIH: A role for sterics in linking substrate binding to O2 activation.
The facial triad in the α-ketoglutarate dependent oxygenase FIH: A role for sterics in linking substrate binding to O2 activation. J Inorg Biochem. 2016 Oct 17;166:26-33 Authors: Hangasky JA, Taabazuing CY, Martin CB, Eron SJ, Knapp MJ Abstract The factor inhibiting hypoxia inducible factor-1α (FIH) is a nonheme Fe(II)/αKG oxygenase using a 2-His-1-Asp facial triad. FIH activates O2 via oxidative decarboxylation of α-ketoglutarate (αKG) to generate an enzyme-based oxidant which hydroxylates the Asn(803) residue within the C-terminal transactivation domain (CTAD) of HIF-1&...
Source: Journal of Inorganic Biochemistry - October 16, 2016 Category: Biochemistry Authors: Hangasky JA, Taabazuing CY, Martin CB, Eron SJ, Knapp MJ Tags: J Inorg Biochem Source Type: research

New dibutyltin(IV) ladders: Syntheses, structures and, optimization and evaluation of cytotoxic potential employing A375 (melanoma) and HCT116 (colon carcinoma) cell lines in vitro.
Abstract Synthesis and spectroscopic properties of seven new dibutyltin(IV) compounds of 2-{(E)-4-hydroxy-3-[(E)-4-(aryl)iminomethyl]phenyldiazenyl}benzoic acids (L(n)HH'; n=2-8) with general formula {[Bu2Sn(L(n)H)]2O}2 (1-7) are reported. The compounds were characterized by elemental analysis and by UV-Visible, fluorescence, IR, (1)H, (13)C and (119)Sn NMR spectroscopies. Solid state structures of dibutyltin(IV) compounds 1-3, 6 and 7 were accomplished from single crystal X-ray crystallography which reveal the common ladder-type structure with two endo- and two exo-Sn atoms. The redox properties of L(n)HH' (n=2-4...
Source: Journal of Inorganic Biochemistry - October 14, 2016 Category: Biochemistry Authors: Basu Baul TS, Dutta D, Duthie A, Guchhait N, Rocha BG, Guedes da Silva MF, Mokhamatam RB, Raviprakash N, Manna SK Tags: J Inorg Biochem Source Type: research

Nitrite coordination in myoglobin.
Abstract The coordination of nitrite in myoglobin (Mb) has been characterized by resonance Raman spectroscopy and the frequencies of the nitrite bound to the heme Fe as well to the 2-vinyl have been computed by density functional theory (DFT) calculations. The DFT Natural Bond Orbital (NBO) analysis and the extensive isotope-labeling in the resonance Raman experiments indicate that NO2(-) (O1NO2) is bound to the heme Fe via O1. Based on the vibrational characterization of the reversible transition between low and high spin FeONO/2-nitrovinyl species, we suggest that the key step that triggers the spin-change is th...
Source: Journal of Inorganic Biochemistry - October 13, 2016 Category: Biochemistry Authors: Ioannou A, Lambrou A, Daskalakis V, Pinakoulaki E Tags: J Inorg Biochem Source Type: research

Rat liver mitochondrial dysfunction by addition of copper(II) or iron(III) ions.
Repetto MG Abstract Increased copper (Cu) and iron (Fe) levels in liver and brain are associated to oxidative stress and damage with increased phospholipid oxidation process. The aim of this work was to assess the toxic effects of Cu(2+) and Fe(3+) addition to rat liver mitochondria by determining mitochondrial respiration in states 3 (active respiration) and 4 (resting respiration), and phospholipid peroxidation. Both, Cu(2+) and Fe(3+) produced decreases in O2 consumption in a concentration-dependent manner in active state 3: both ions by 42% with malate-glutamate as complex I substrate (concentration for half ...
Source: Journal of Inorganic Biochemistry - October 13, 2016 Category: Biochemistry Authors: Saporito-Magriñá C, Musacco-Sebio R, Acosta JM, Bajicoff S, Paredes-Fleitas P, Boveris A, Repetto MG Tags: J Inorg Biochem Source Type: research

Synthesis, crystal structures, DFT studies, molecular docking and urease inhibition studies of three Ni(II) complexes with a sexidentate N2O4-donor bis-Schiff base ligand.
Abstract Three Ni(II) complexes with a sexidentate N2O4-donor bis-Schiff base ligand, namely Ni(C36H34N2O8)·2CH3OH (1), Ni(C28H34N2O8S2)·H2O (2) and Ni(C40H36N4O8)·3CH3OH (3) (C36H34N2O8=1,2-bis(2-methoxy-6-formylphenoxy)ethane-l-phenylalanine; C28H34N2O8S2=1,2-bis(2-methoxy-6-formylphenoxy)ethane-l-methionine; C40H36N4O8=1,2-bis(2-methoxy-6-formylphenoxy)ethane-l-tryptophan) were synthesized and structurally characterized. Theoretical studies of the three complexes were carried out by density functional theory (DFT) Becke's three-parameter hybrid (B3LYP) method employing the 6-31G basis set. ...
Source: Journal of Inorganic Biochemistry - October 13, 2016 Category: Biochemistry Authors: Wang H, Lan T, Zhang X, Zhang D, Bi C, Fan Y Tags: J Inorg Biochem Source Type: research

Biochemical characterization and zinc binding group (ZBGs) inhibition studies on the catalytic domain of MMP7 (cdMMP7).
This study describes a well-characterized analog of MMP7 that is available for future inhibitor design efforts. PMID: 27755977 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - October 13, 2016 Category: Biochemistry Authors: Meng F, Yang H, Jack C, Zhang H, Moller A, Spivey D, Page RC, Tierney DL, Crowder MW Tags: J Inorg Biochem Source Type: research

Selective speciation improves efficacy and lowers toxicity of platinum anticancer and vanadium antidiabetic drugs.
Abstract Improving efficacy and lowering resistance to metal-based drugs can be addressed by consideration of the coordination complex speciation and key reactions important to vanadium antidiabetic drugs or platinum anticancer drugs under biological conditions. The methods of analyses vary depending on the specific metal ion chemistry. The vanadium compounds interconvert readily, whereas the reactions of the platinum compounds are much slower and thus much easier to study. However, the vanadium species are readily differentiated due to vanadium complexes differing in color. For both vanadium and platinum systems,...
Source: Journal of Inorganic Biochemistry - October 2, 2016 Category: Biochemistry Authors: Doucette KA, Hassell KN, Crans DC Tags: J Inorg Biochem Source Type: research

Antiproliferative properties and biomolecular interactions of three Pd(II) and Pt(II) complexes.
Abstract Three Pd(II) and Pt(II) complexes with chelating mono(imidazolin-2-imine) and bis(imidazolin-2-imine) ligands i.e. [Pd(DMEAIm(iPr))Cl2] (1) (DMEAIm(iPr), 2-(1,3-diisopropyl-4,5-dimethylimidazolin-2-imine)ethan-1-dimethylamine), [Pd(DACH(Im(iPr))2)Cl2] (2) (DACH(Im(iPr))2, N,N'-(cyclohexane-1,2-diyl)bis(1,3-diisopropyl-4,5-dimethylimidazolin-2-imine)) and [Pt(DMEAIm(iPr))Cl2] (3), are evaluated here as potential cytotoxic and anticancer agents. An acceptable solution behaviour was found for the three study compounds in terms of solubility and stability. Notably, the three metal complexes demonstrated moder...
Source: Journal of Inorganic Biochemistry - September 29, 2016 Category: Biochemistry Authors: Massai L, Pratesi A, Bogojeski J, Banchini M, Pillozzi S, Messori L, Bugarčić ŽD Tags: J Inorg Biochem Source Type: research

Copper (II) and zinc (II) complexes with flavanone derivatives: Identification of potential cholinesterase inhibitors by on-flow assays.
Abstract Metal chelates strongly influence the nature and magnitude of pharmacological activities in flavonoids. In recent years, studies have shown that a promising class of flavanone-metal ion complexes can act as selective cholinesterase inhibitors (ChEIs), which has led our group to synthesize a new series of flavanone derivatives (hesperidin, hesperetin, naringin, and naringenin) complexed to either copper (II) or zinc (II) and to evaluate their potential use as selective ChEIs. Most of the synthesized complexes exhibited greater inhibitory activity against acetylcholinesterase (AChE) than against butyrylchol...
Source: Journal of Inorganic Biochemistry - September 16, 2016 Category: Biochemistry Authors: Sarria AL, Vilela AF, Frugeri BM, Fernandes JB, Carlos RM, da Silva MF, Cass QB, Cardoso CL Tags: J Inorg Biochem Source Type: research

Role of subcellular calcium redistribution in regulating apoptosis and autophagy in cadmium-exposed primary rat proximal tubular cells.
Abstract Ca(2+) signaling plays a vital role in regulating apoptosis and autophagy. We previously proved that cytosolic Ca(2+) overload is involved in cadmium (Cd)-induced apoptosis in rat proximal tubular (rPT) cells, but the source of elevated cytosolic Ca(2+) concentration ([Ca(2+)]c) and the effect of potential subcellular Ca(2+) redistribution on apoptosis and autophagy remain to be elucidated. Firstly, data showed that Cd-induced elevation of [Ca(2+)]c was primarily generated intracellularly. Moreover, elevations of [Ca(2+)]c and mitochondrial Ca(2+) concentration ([Ca(2+)]mit) with depletion of endoplasmic ...
Source: Journal of Inorganic Biochemistry - September 13, 2016 Category: Biochemistry Authors: Liu F, Li ZF, Wang ZY, Wang L Tags: J Inorg Biochem Source Type: research

Effects of metal ions and cosolutes on G-quadruplex topology.
oto N Abstract Topologies of G-quadruplexes depend on oligonucleotide sequences and on environmental factors, and the diversity of G-quadruplex topologies complicates investigation of functions of these nucleic acid structures. To investigate how metal ions and cosolutes regulate topologies of G-quadruplexes, we stabilized the antiparallel conformation by insertion of 2'-deoxyxanthosine and 8-oxo-2'-deoxyguanosine into selected positions of an oligonucleotide. Thermodynamic analyses of the oligonucleotide revealed that Na(+) stabilized the antiparallel G-quadruplex, whereas K(+) destabilized this topology. This re...
Source: Journal of Inorganic Biochemistry - September 13, 2016 Category: Biochemistry Authors: Fujii T, Podbevšek P, Plavec J, Sugimoto N Tags: J Inorg Biochem Source Type: research

Chemical properties and biotoxicity of several chromium picolinate derivatives.
Abstract As a man-made additive, chromium picolinate Cr(pic)3 has become a popular dietary supplement worldwide. In this paper Cr(pic)3 and its new derivatives Cr(6-CH3-pic)3 (1), [Cr(6-NH2-pic)2(H2O)2]NO3 (2) and Cr(3-NH2-pic)3 (3) were synthesized, and complexes 1 and 2 were characterized by X-ray crystal structure (where pic=2-carboxypyridine). The relationship between the chemical properties and biotoxicity of these complexes was fully discussed: (1) The dynamics stability of chromium picolinate complexes mainly depends on the CrN bonds length. (2) There is a positive correlation between the dynamics stability...
Source: Journal of Inorganic Biochemistry - September 13, 2016 Category: Biochemistry Authors: Liu B, Liu Y, Chai J, Hu X, Wu D, Yang B Tags: J Inorg Biochem Source Type: research

Synthesis and biological evaluation of PEGylated CuO nanoparticles.
Abstract There is a growing field of research into the physicochemical properties of metal oxide nanoparticles (NPs) and their potential use against tumor formation, development and progression. Coated NPs with biocompatible surfactants can be incorporated into the natural metabolic pathway of the body and specifically favor delivery to the targeted cancerous cells versus normal cells. Polyethylene glycol (PEG) is an FDA approved, biocompatible synthetic polymer and PEGylated NPs are regarded as "stealth" nanoparticles, which are not recognized by the immune system. Herein, PEGylated cupric oxide nanopar...
Source: Journal of Inorganic Biochemistry - September 13, 2016 Category: Biochemistry Authors: Giannousi K, Hatzivassiliou E, Mourdikoudis S, Vourlias G, Pantazaki A, Dendrinou-Samara C Tags: J Inorg Biochem Source Type: research

Ru(II) complexes bearing guanidinium ligands as potent anticancer agents.
Abstract Two ruthenium(II) complexes containing guanidinium ligands have been synthesized and characterized for the first time. It was found that the two complexes exhibit moderate antitumor activity in Hela, A549, CNE-2, MCF-7, and HepG2 human tumor cells. Flow cytometric analysis showed that both complexes arrested the cell cycle in the G2/M phase and induced apoptosis in Hela cells. Mechanism studies indicate that both complexes induced apoptosis through caspase- and reactive oxygen species (ROS)-dependent pathways. Additionally, the two complexes displayed higher phototoxicity to tumor cells and almost no infl...
Source: Journal of Inorganic Biochemistry - September 13, 2016 Category: Biochemistry Authors: Chen WX, Song XD, He SF, Sun J, Chen JX, Wu T, Mao ZW Tags: J Inorg Biochem Source Type: research

Volumetric analysis of formation of the complex of G-quadruplex DNA with hemin using high pressure.
Abstract DNA guanine-quadruplexes (G-quadruplexes) complexed with the Fe-containing porphyrin, hemin (iron(III)-protoporphyrin IX), can catalyze oxidation reactions. This so-called DNAzyme has been widely used in the field of DNA nanotechnology. To improve DNAzyme properties, we sought to elucidate the interaction mechanism between G-quadruplex DNA and hemin. Here, we performed volumetric analyses of formation of the complex between an oligonucleotide with the sequence of human telomeric DNA (h-telo) and hemin. The G-quadruplex DNA alone and the G-quadruplex DNA-hemin complex were destabilized with increasing pres...
Source: Journal of Inorganic Biochemistry - September 1, 2016 Category: Biochemistry Authors: Takahashi S, Bhowmik S, Sugimoto N Tags: J Inorg Biochem Source Type: research

Controlled coordination in vanadium(V) dimethylhydrazido compounds.
Abstract The vanadium(V) dimethylhydrazido compounds were structurally characterized to elucidate the effect of the alkoxide ligands in the coordination environment of vanadium(V) hydrazido center. The single-crystal X-ray structure determination of the vanadium(V) dimethylhydrazido compound with isopropoxide ligands revealed a dimeric structure with the V(1)-N(1) distance of 1.680(5)Å, in which each vanadium atom is coordinated in a distorted trigonal-bipyramidal geometry (τ5=0.81) with the hydrazido and bridging isopropoxide ligands in the apical positions. On the contrary, nearly tetrahedral arrangeme...
Source: Journal of Inorganic Biochemistry - August 28, 2016 Category: Biochemistry Authors: Sakuramoto T, Moriuchi T, Hirao T Tags: J Inorg Biochem Source Type: research

Globin domain interactions control heme pocket conformation and oligomerization of globin coupled sensors.
Abstract Globin coupled sensors (GCS) are O2-sensing proteins used by bacteria to monitor the surrounding gaseous environment. To investigate the biphasic O2 dissociation kinetics observed for full-length GCS proteins, isolated globin domains from Pectobacterium carotovorum ssp. carotovorum (PccGlobin), and Bordetella pertussis (BpeGlobin), have been characterized. PccGlobin is found to be dimeric, while BpeGlobin is monomeric, indicating key differences in the globin domain dimer interface. Through characterization of wild type globin domains and globin variants with mutations at the dimer interface and within th...
Source: Journal of Inorganic Biochemistry - August 26, 2016 Category: Biochemistry Authors: Rivera S, Burns JL, Vansuch GE, Chica B, Weinert EE Tags: J Inorg Biochem Source Type: research

Influence of the N-terminus acetylation of Semax, a synthetic analog of ACTH(4-10), on copper(II) and zinc(II) coordination and biological properties.
Attanasio F Abstract Semax is a heptapeptide (Met-Glu-His-Phe-Pro-Gly-Pro) that encompasses the sequence 4-7 of N-terminal domain of the adrenocorticotropic hormone and a C-terminal Pro-Gly-Pro tripeptide. N-terminal amino group acetylation (Ac-Semax) modulates the chemical and biological properties of parental peptide, modifying the ability of Semax to form complex species with Cu(II) ion. At physiological pH, the main complex species formed by Ac-Semax, [CuLH-2](2-), consists in a distorted CuN3O chromophore with a weak apical interaction of the methionine sulphur. Such a complex differs from the Cu(II)-Semax co...
Source: Journal of Inorganic Biochemistry - August 26, 2016 Category: Biochemistry Authors: Magrì A, Tabbì G, Giuffrida A, Pappalardo G, Satriano C, Naletova I, Nicoletti VG, Attanasio F Tags: J Inorg Biochem Source Type: research

Triphenylphosphane Pt(II) complexes containing biologically active natural polyphenols: Synthesis, crystal structure, molecular modeling and cytotoxic studies.
Abstract Platinum complexes bearing phosphane ligands in cis configuration with deprotonated flavonoids (3-hydroxyflavone, quercetin) and deprotonated ethyl gallate were synthesized starting from cis-[PtCl2(PPh3)2]. In all cases, O,O' chelate structures were obtained. While quercetin and ethyl gallate complexes are quite stable in solution, the 3-hydroxyflavonate complex undergoes a slow aerobic photodegradation in solution with formation of salicylic and benzoic acids. The X-ray diffraction structures of quercetin and ethyl gallate complexes are reported. Cell cycle studies (in the dark) of the complexes in two h...
Source: Journal of Inorganic Biochemistry - August 8, 2016 Category: Biochemistry Authors: Dell'Anna MM, Censi V, Carrozzini B, Caliandro R, Denora N, Franco M, Veclani D, Melchior A, Tolazzi M, Mastrorilli P Tags: J Inorg Biochem Source Type: research

Near-infrared luminescence and RNA cleavage ability of lanthanide Schiff base complexes derived from N,N'-bis(3-methoxysalicylidene)ethylene-1,2-diamine ligands.
Abstract A complete series of lanthanide Schiff base salen-type complexes were prepared with trivalent lanthanide ions (Ln(3+)) and the N,N'-bis-(3-methoxysalicylidene)ethylene-1,2-diamine ligand (Ln(3+)=La(3+), Ce(3+), Pr(3+), Nd(3+), Sm(3+), Eu(3+), Gd(3+), Tb(3+), Dy(3+), Ho(3+), Er(3+), Tm(3+), Yb(3+), Lu(3+)). Three unique crystal structures of La(3+) and Pr(3+)N,N'-bis-(3-methoxysalicylidene)ethylene-1,2-diamine complexes, with the La(3+) complex prepared in two different synthetic approaches, are reported, namely a dimeric [La(H2L)(NO3)3]2 (H2L=N,N'-bis-(3-methoxysalicylidene)ethylene-1,2-diamine) complex, ...
Source: Journal of Inorganic Biochemistry - August 4, 2016 Category: Biochemistry Authors: Kaczmarek AM, Porebski PW, Mortier T, Lynen F, Van Deun R, Van Hecke K Tags: J Inorg Biochem Source Type: research

Visible light-induced formation of corrole-manganese(V)-oxo complexes: Observation of multiple oxidation pathways.
Abstract Two manganese(V)-oxo corroles [Mn(V)(Cor)O] that differ in their electronic environments were produced by visible light irradiation of highly photo-labile corrole-manganese(IV) bromates. The corrole ligands under study include 5,10,15-tris(pentafluorophenyl)corrole (TPFC), and 5,10,15-triphenylcorrole (TPC). The kinetics of oxygen transfer atom (OAT) reactions with various organic reductants by these photo-generated Mn(V)(Cor)O were also studied in CH3CN and CH2Cl2 solutions. Mn(V)(Cor)O exhibits remarkable solvent and ligand effect on its reactivity and spectral behavior. In the more electron-deficient T...
Source: Journal of Inorganic Biochemistry - August 4, 2016 Category: Biochemistry Authors: Ka WK, Ngo FL, Ranburger D, Malone J, Zhang R Tags: J Inorg Biochem Source Type: research

Design, syntheses, characterization, and cytotoxicity studies of novel heterobinuclear oxindolimine copper(II)-platinum(II) complexes.
Abstract Herein, the design and syntheses of two new mononuclear oxindolimine-copper(II) (1 and 2) and corresponding heterobinuclear oxindolimine Cu(II)Pt(II) complexes (3 and 4), are described. All the isolated complexes were characterized by spectroscopic techniques (UV/Vis, IR, EPR), in addition to elemental analysis and mass spectrometry. Cyclic voltammetry (CV) measurements showed that in all cases, one-electron quasi-reversible waves were observed, and ascribed to the formation of corresponding copper(I) complexes. Additionally, waves related to oxindolimine ligand reduction was verified, and confirmed using...
Source: Journal of Inorganic Biochemistry - August 2, 2016 Category: Biochemistry Authors: Aranda EE, Matias TA, Araki K, Vieira AP, de Mattos EA, Colepicolo P, Luz CP, Marques FL, da Costa Ferreira AM Tags: J Inorg Biochem Source Type: research

Cobalt(II) complexes of sparfloxacin: Characterization, structure, antimicrobial activity and interaction with DNA and albumins.
Abstract The cobalt(II) complexes with the quinolone sparfloxacin (Hsf) in the absence or presence of the nitrogen-donor heterocyclic ligands 2,2'-bipyridine (bipy), 1,10-phenanthroline (phen) or 2,2'-bipyridylamine (bipyam) were prepared and characterized physicochemically and spectroscopically. The crystal structures of complexes [Co(sf)2(bipy)]∙3MeOH∙2H2O and [Co(sf)2(phen)]∙4MeOH were determined by X-ray crystallography. The antimicrobial activity of the complexes was tested against four different microorganisms (Escherichia coli, Xanthomonas campestris, Staphylococcus aureus and Bacillus subtilis) and w...
Source: Journal of Inorganic Biochemistry - July 27, 2016 Category: Biochemistry Authors: Kouris E, Kalogiannis S, Perdih F, Turel I, Psomas G Tags: J Inorg Biochem Source Type: research

Design and cellular studies of a carbon nanotube-based delivery system for a hybrid platinum-acridine anticancer agent.
Abstract A three-component drug-delivery system has been developed consisting of multi-walled carbon nanotubes (MWCNTs) coated with a non-classical platinum chemotherapeutic agent ([PtCl(NH3)2(L)]Cl (P3A1; L=N-(2-(acridin-9-ylamino)ethyl)-N-methylproprionimidamide) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-5000] (DSPE-mPEG). The optimized P3A1-MWCNTs are colloidally stable in physiological solution and deliver more P3A1 into breast cancer cells than treatment with the free drug. Furthermore, P3A1-MWCNTs are cytotoxic to several cell models of breast cancer and induce S-phase...
Source: Journal of Inorganic Biochemistry - July 26, 2016 Category: Biochemistry Authors: Fahrenholtz CD, Ding S, Bernish BW, Wright ML, Zheng Y, Yang M, Yao X, Donati GL, Gross MD, Bierbach U, Singh R Tags: J Inorg Biochem Source Type: research

Effects of histidin-2-ylidene vs. imidazol-2-ylidene ligands on the anticancer and antivascular activity of complexes of ruthenium, iridium, platinum, and gold.
Abstract Couples of N-heterocyclic carbene complexes of ruthenium, iridium, platinum, and gold, each differing only in the carbene ligand being either 1,3-dimethylimidazol-2-ylidene (IM) or 1,3-dimethyl-N-boc-O-methylhistidin-2-ylidene (HIS), were assessed for their antiproliferative effect on seven cancer cell lines, their interaction with DNA, their cell cycle interference, and their vascular disrupting properties. In MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays only the platinum complexes were cytotoxic at single-digit micromolar IC50 concentrations with the (HIS)Pt complex being on...
Source: Journal of Inorganic Biochemistry - July 26, 2016 Category: Biochemistry Authors: Schmitt F, Donnelly K, Muenzner JK, Rehm T, Novohradsky V, Brabec V, Kasparkova J, Albrecht M, Schobert R, Mueller T Tags: J Inorg Biochem Source Type: research

EPR and DFT analysis of biologically relevant chromium(V) complexes with d-glucitol and d-glucose.
ez JC Abstract 1,2-diolato ligands, such as carbohydrates and glycoproteins, tend to stabilize chromium(V), thus forming important intermediates that have been implicated in the genotoxicity of Cr(VI). Since many years, room-temperature continuous-wave electron paramagnetic resonance (EPR) at X-band microwave frequencies has been used as a standard characterization tool to study chromium(V) intermediates formed during the reduction of Cr(VI) in the presence of biomolecules. In this work, the added value is tested of using a combination of pulsed and high-field EPR techniques with density functional theory computat...
Source: Journal of Inorganic Biochemistry - July 19, 2016 Category: Biochemistry Authors: Van Doorslaer S, Beirinckx Q, Nys K, Mangiameli MF, Cuypers B, Callens F, Vrielinck H, González JC Tags: J Inorg Biochem Source Type: research

High stability and biological activity of the copper(II) complexes of alloferon 1 analogues containing tryptophan.
Abstract Copper(II) complex formation processes between the alloferon 1 (Allo1) (HGVSGHGQHGVHG) analogues where the tryptophan residue is introducing in the place His residue H1W, H6W, H9W and H12W have been studied by potentiometric, UV-visible, CD and EPR spectroscopic, and MS methods. For all analogues of alloferon 1 complex speciation have been obtained for a 1:1 metal-to-ligand molar ratio and 2:1 of H1W because of precipitation at higher (2:1, 3:1 and 4:1) ratios. At physiological pH7.4 and a 1:1 metal-to-ligand molar ratio the tryptophan analogues of alloferon 1 form the CuH-1L and/or CuH-2L complexes with ...
Source: Journal of Inorganic Biochemistry - July 8, 2016 Category: Biochemistry Authors: Kadej A, Kuczer M, Czarniewska E, Urbański A, Rosiński G, Kowalik-Jankowska T Tags: J Inorg Biochem Source Type: research

Synthesis, characterization and activity of imidazolate-bridged and Schiff-base dinuclear complexes as models of Cu,Zn-SOD. A comparative study.
la SR Abstract Two imidazolate-bridged diCu(II) and Cu(II)Zn(II) complexes, [CuZn(dien)2(μ-Im)](ClO4)3·MeOH (1) and [Cu2(dien)2(μ-Im)](ClO4)3 (2) (Im = imidazole, dien=diethylenetriamine), and two complexes formed with Schiff base ligands, [CuZn(salpn)Cl2] (3) and [Cu2(salbutO)ClO4] (4) (H2salpn=1,3-bis(salicylidenamino)propane, H3salbutO=1,4-bis(salicylidenamino)butan-2-ol) have been prepared and characterized. The reaction of [Cu(dien)(ImH)](ClO4)2 with [Zn(dien)(H2O)](ClO4)2 at pH≥11 yields complex 1; at lower pH, the Cu3Zn tetranuclear complex [{(dien)Cu(μ-Im)}3Zn(OH2)(ClO4)2](ClO4)3 (1a) fo...
Source: Journal of Inorganic Biochemistry - July 8, 2016 Category: Biochemistry Authors: Daier VA, Rivière E, Mallet-Ladeira S, Moreno DM, Hureau C, Signorella SR Tags: J Inorg Biochem Source Type: research

Synthesis and antimicrobial activity of tetradentate ligands bearing hydrazone and/or thiosemicarbazone motifs and their diorganotin(IV) complexes.
cute;pez-Torres E Abstract Four novel ligands derived from 2,3-butanedione have been synthesized, two dissymmetric thiosemicarbazone/3-hydroxy-2-naphthohydrazone ligands, H2L(1) (bearing 4-isopropyl-3-thiosemicarbazone) and H2L(2) (containing 4-cyclohexyl-3-thiosemicarbazone) and the symmetric H2L(3), diacetyl bis(3-hydroxy-2-naphthohydrazone), and H2L(4), diacetyl bis(4-cyclohexyl-3-thiosemicarbazone). Their reactivity with SnR2Cl2 (R=methyl, n-butyl and phenyl) was explored and the resulting complexes were characterized by elemental analysis, molar conductivity, mass spectrometry, IR, (1)H, (13)C and (119)Sn NMR...
Source: Journal of Inorganic Biochemistry - July 7, 2016 Category: Biochemistry Authors: González-García C, Mata A, Zani F, Mendiola MA, López-Torres E Tags: J Inorg Biochem Source Type: research

Preface for the COST Action Special Issue.
r J PMID: 27325582 [PubMed - in process] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - June 23, 2016 Category: Biochemistry Authors: Barea E, Müller J Tags: J Inorg Biochem Source Type: research

Covalent binding and non-covalent binding of metal complexes to RNA.
Abstract Targeting nucleic acids with metal complexes is an exciting and widely explored field of research. Following the discovery of the anticancer drug cisplatin, a number of metal complexes have been designed, synthesised, and tested for their DNA binding properties. On the contrary, the interaction of metal complexes with RNA has been much less investigated. RNA is an essential biomolecule, involved in a variety of crucial cellular functions, which offers a much wider structural diversity than DNA. As such, RNA represents an attractive target for the design and the development of structure-selective therapeut...
Source: Journal of Inorganic Biochemistry - June 7, 2016 Category: Biochemistry Authors: Alberti E, Zampakou M, Donghi D Tags: J Inorg Biochem Source Type: research

Interactions of diamines with adenosine-5'-triphosphate (ATP) in the systems including copper(II) ions.
Abstract Interactions were studied in the systems ATP/tn and ATP/Put (tn=1,3-diaminopropane, Put=putrescine) whereas the complexation reactions in ternary systems Cu(II)/ATP/tn and Cu(II)/ATP/Put. Results of the potentiometric and spectroscopic studies evidenced the formation of adducts of the type (ATP)Hx(PA), where PA=diamine. The thermodynamic stability of the complexes and the mode of interactions were determined. On the basis of analysis of changes in the positions of NMR signals, in the pH range of (ATP)H3(Put) formation, the preferred centres of the interaction between ATP and Put are the endocyclic nitroge...
Source: Journal of Inorganic Biochemistry - June 3, 2016 Category: Biochemistry Authors: Bregier-Jarzebowska R, Gasowska A, Hoffmann SK, Lomozik L Tags: J Inorg Biochem Source Type: research

High kinetic stability of Zn(II) coordinated by the tris(histidine) unit of carbonic anhydrase towards solvolytic dissociation studied by affinity capillary electrophoresis.
Abstract Solvolytic dissociation rate constants (kd) of bovine carbonic anhydrase II (CA) and its metallovariants (M-CAs, M=Co(II), Ni(II), Cu(II), Zn(II), and Cd(II)) were estimated by a ligand substitution reaction, which was monitored by affinity capillary electrophoresis to selectively detect the undissociated CAs in the reaction mixture. Using EDTA as the competing ligand for Zn-CA, the dissociation followed the unimolecular nucleophilic substitution (SN1) mechanism with kd=1.0×10(-7)s(-1) (pH7.4, 25°C). The corresponding solvolysis half-life (t1/2) was 80days, showing the exceptionally high kinetic...
Source: Journal of Inorganic Biochemistry - May 16, 2016 Category: Biochemistry Authors: Sato Y, Hoshino H, Iki N Tags: J Inorg Biochem Source Type: research

A comparative study on the interactions of human copper chaperone Cox17 with anticancer organoruthenium(II) complexes and cisplatin by mass spectrometry.
Abstract Herein we report investigation of the interactions between anticancer organoruthenium complexes, [(η(6)-arene)Ru(en)(Cl)]PF6 (en=ethylenediamine, arene=p-cymene (1) or biphenyl (2)), and the human copper chaperone protein Cox17 by mass spectrometry with cisplatin as a reference. The electrospray ionization mass spectrometry (ESI-MS) results indicate much weaker binding of the ruthenium complexes than that of cisplatin to apo-Cox172s-s, the functional state of Cox17. Up to tetra-platinated Cox17 adducts were identified while only mono-ruthenated and a little amount of di-ruthenated Cox17 adducts were d...
Source: Journal of Inorganic Biochemistry - May 14, 2016 Category: Biochemistry Authors: Li L, Guo W, Wu K, Wu X, Zhao L, Zhao Y, Luo Q, Wang Y, Liu Y, Zhang Q, Wang F Tags: J Inorg Biochem Source Type: research

G-quadruplex vs. duplex-DNA binding of nickel(II) and zinc(II) Schiff base complexes.
Abstract Novel nickel(II) (1) and zinc(II) (2) complexes of a Salen-like ligand, carrying a pyrimidine ring on the N,N' bridge, were synthesized and characterized. Their interaction with duplex and G-quadruplex DNA was investigated in aqueous solution through UV-visible absorption, circular dichroism and viscometry measurements. The results obtained point out that, while the zinc(II) complex does not interact with both duplex and G-quadruplex DNA, the nickel(II) complex 1 binds preferentially to G-quadruplex respect to duplex-DNA, with values of the DNA-binding constants, Kb, 2.6×10(5)M(-1) and 3.5×10(...
Source: Journal of Inorganic Biochemistry - May 13, 2016 Category: Biochemistry Authors: Bonsignore R, Terenzi A, Spinello A, Martorana A, Lauria A, Almerico AM, Keppler BK, Barone G Tags: J Inorg Biochem Source Type: research

Revised stability constant, spectroscopic properties and binding mode of Zn(II) to FluoZin-3, the most common zinc probe in life sciences.
Abstract 2-[2-[2-[2-[bis(carboxylatomethyl)amino]-5-methoxyphenoxy]ethoxy]-4-(2,7-difluoro-3-oxido-6-oxo-4a,9a-dihydroxanthen-9-yl)anilino]acetate (FluoZin-3) is used very broadly in life sciences as intra- and extracellular Zn(II) sensor selective for Zn(II) over Co(II), Ca(II) and Mg(II) ions at their physiological concentrations. It has been used for determination of relative and absolute levels of exchangeable Zn(II) in cells and extracellular fluids. Despite its popularity, the knowledge of its acid/base and Zn(II) coordination abilities and of its spectroscopic properties remained very limited. Also the publ...
Source: Journal of Inorganic Biochemistry - May 12, 2016 Category: Biochemistry Authors: Marszałek I, Krężel A, Goch W, Zhukov I, Paczkowska I, Bal W Tags: J Inorg Biochem Source Type: research

Surface complex of ZnTMPyP4 metalloporphyrin with double-stranded Poly(A)-Poly(U).
Abstract This communication presents synthesis and spectral characterization of metalloporphyrin [Zn(X)TMPyP4] (TMPyP4 is 5,10,15,20-tetrakis (N-methylpyridinium-4-yl)porphyrin), and studies its binding onto anionic surface sites of synthetic double stranded polynucleotide Poly(A)-Poly(U). [Zn(X)TMPyP4] binding with Poly(A)-Poly(U) was monitored by UV-Vis absorbance spectroscopy, two fluorescence spectroscopies and 1H NMR in a working aqueous medium of 0.15M ionic strength, pH7.0 and at 25°C. The evidence provided by spectroscopic measurements and multivariate data analysis suggests the use of this metalloporp...
Source: Journal of Inorganic Biochemistry - May 5, 2016 Category: Biochemistry Authors: Tolstykh G, Sizov V, Kudrev A Tags: J Inorg Biochem Source Type: research

Effects of Cu(II) and cisplatin on the stability of Specific protein 1 (Sp1)-DNA binding: Insights into the regulation of copper homeostasis and platinum drug transport.
Abstract The human high-affinity copper transporter 1 (hCtr1) transports both Cu(I) and cisplatin (cDDP). Because Cu deficiency is lethal yet Cu overload is poisonous, hCtr1 expression is transcriptionally upregulated in response to Cu deficiency but is downregulated under Cu replete conditions in controlling Cu homeostasis. The up- and down-regulation of hCtr1 is regulated by Specific protein 1 (Sp1), which itself is also correspondingly regulated under these Cu conditions. hCtr1 expression is also upregulated by cDDP via upregulation of Sp1. The underlying mechanisms of these regulations are unknown. Using gel-e...
Source: Journal of Inorganic Biochemistry - April 26, 2016 Category: Biochemistry Authors: Yan D, Aiba I, Chen HH, Kuo MT Tags: J Inorg Biochem Source Type: research

Zinc complexes of flufenamic acid: Characterization and biological evaluation.
Abstract The reaction of ZnCl2 with the non-steroidal anti-inflammatory drug flufenamic acid (Hfluf) led to the formation of complex [Zn(fluf-O)2(MeOH)4], 1. When the reaction takes places in the presence of a N,N'-donor heterocyclic ligand such as 2.2'-bipyridylamine (bipyam), 2.2'-bipyridine (bipy), 1.10-phenanthroline (phen) and 2.2'-dipyridylketone oxime (Hpko), the complexes [Zn(fluf)2(bipyam)], 2, [Zn(fluf)2(bipy)], 3, [Zn(fluf)(phen)2(H2O)](fluf)·0.2MeOH, 4·0.2MeOH and [Zn(fluf)2(Hpko)2], 5 were isolated, respectively. The complexes were characterized by physicochemical and spectroscopic techn...
Source: Journal of Inorganic Biochemistry - April 21, 2016 Category: Biochemistry Authors: Tarushi A, Kastanias P, Raptopoulou CP, Psycharis V, Kessissoglou DP, Papadopoulos AN, Psomas G Tags: J Inorg Biochem Source Type: research

Synthesis, structural characterization, cytotoxic properties and DNA binding of a dinuclear copper(II) complex.
In this study a novel dinuclear copper(II) complex with adenine and phenanthroline has been synthesized and its structure determined by single crystal X-ray diffraction. In the dinuclear complex [Cu₂(μ-adenine)₂(phen)₂(H2O)2](NO3)4·0.5H2O (phen=1,10-phenanthroline) (1) the two Cu(II) centres exhibit a distorted square pyramidal coordination geometry linked by two nitrogen donors from adenine bridges leading to a Cu-Cu distance of 3.242(3)Å. Intramolecular and intermolecular π⋯π interactions as well as an H-bonding network were observed. The antitumor capacity of the complex has been tested in ...
Source: Journal of Inorganic Biochemistry - April 20, 2016 Category: Biochemistry Authors: Ferreira BJ, Brandão P, Meireles M, Martel F, Correia-Branco A, Fernandes DM, Santos TM, Félix V Tags: J Inorg Biochem Source Type: research

Multifunctional quinoline-triazole derivatives as potential modulators of amyloid-β peptide aggregation.
Multifunctional quinoline-triazole derivatives as potential modulators of amyloid-β peptide aggregation. J Inorg Biochem. 2016 Apr 16; Authors: Jones MR, Dyrager C, Hoarau M, Korshavn KJ, Lim MH, Ramamoorthy A, Storr T PMID: 27133802 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - April 15, 2016 Category: Biochemistry Authors: Jones MR, Dyrager C, Hoarau M, Korshavn KJ, Lim MH, Ramamoorthy A, Storr T Tags: J Inorg Biochem Source Type: research

Oxidative stress protection by manganese complexes of tail-tied aza-scorpiand ligands.
iansó-Llort L, Serena C, Trefler S, Garcia-España A Abstract The Mn(2+) coordination chemistry of double scorpiand ligands in which two polyazacyclophane macrocycles have been connected by pyridine, phenanthroline and bipyridine spacers has been studied by potentiometry, paramagnetic NMR and electrochemistry. All ligands show high stability with Mn(2+) and the complexes were formed in a wide pH range. DFT calculations support the structures and coordination geometries derived from the study. A remarkable antioxidant activity was evidenced for these systems by the McCord-Fridovich assay and in Escheri...
Source: Journal of Inorganic Biochemistry - April 15, 2016 Category: Biochemistry Authors: González-García J, Martínez-Camarena À, Verdejo B, Clares MP, Soriano C, García-España E, Jiménez HR, Doménech-Carbó A, Tejero R, Calvo E, Briansó-Llort L, Serena C, Trefler S, Garcia-España A Tags: J Inorg Biochem Source Type: research

Divergent assembly mechanisms of the manganese/iron cofactors in R2lox and R2c proteins.
x N Abstract A manganese/iron cofactor which performs multi-electron oxidative chemistry is found in two classes of ferritin-like proteins, the small subunit (R2) of class Ic ribonucleotide reductase (R2c) and the R2-like ligand-binding oxidase (R2lox). It is unclear how a heterodimeric Mn/Fe metallocofactor is assembled in these two related proteins as opposed to a homodimeric Fe/Fe cofactor, especially considering the structural similarity and proximity of the two metal-binding sites in both protein scaffolds and the similar first coordination sphere ligand preferences of Mn(II) and Fe(II). Using EPR and Mö...
Source: Journal of Inorganic Biochemistry - April 15, 2016 Category: Biochemistry Authors: Kutin Y, Srinivas V, Fritz M, Kositzki R, Shafaat HS, Birrell J, Bill E, Haumann M, Lubitz W, Högbom M, Griese JJ, Cox N Tags: J Inorg Biochem Source Type: research

High-frequency electron paramagnetic resonance of metal-containing porphyrin compounds using a microcantilever.
In this study, we applied this technique to multi-frequency HFEPR measurements of metal-containing porphyrin compounds, which are an important composing element of metal-containing proteins and coenzymes such as hemoglobin and cyanocobalamin. PMID: 27112897 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - April 15, 2016 Category: Biochemistry Authors: Ohmichi E, Okamoto T, Mitani M, Takahashi H, Ohta H Tags: J Inorg Biochem Source Type: research

Synthesis of novel Iron(III) chelators based on triaza macrocycle backbone and 1-hydroxy-2(H)-pyridin-2-one coordinating groups and their evaluation as antimicrobial agents.
ard D Abstract Several novel chelators based on 1-hydroxy-2(1H)-pyridinone coordinating groups decorating a triaza macrocyclic backbone scaffold were synthesised as potential powerful Fe(3+) chelators capable of competing with bacterial siderophores. In particular, a novel chloromethyl derivative of 1-hydroxy-2(1H)-pyridinone exploiting a novel protective group for this family of coordinating groups was developed. These are the first examples of hexadentate chelators based on 1-hydroxy-2(1H)-pyridinone to be shown to have a biostatic activity against a range of pathogenic bacteria. Their efficacy as biostatic agen...
Source: Journal of Inorganic Biochemistry - April 15, 2016 Category: Biochemistry Authors: Workman DG, Hunter M, Dover LG, Tétard D Tags: J Inorg Biochem Source Type: research

Effects of mutations in active site heme ligands on the spectroscopic and catalytic properties of SoxAX cytochromes.
Abstract By attaching a sulfur substrate to a conserved cysteine of the SoxYZ carrier protein SoxAX cytochromes initiate the reaction cycle of the Sox (sulfur oxidation) multienzyme complex, which is the major pathway for microbial reoxidation of sulfur compounds in the environment. Despite their important role in this process, the reaction mechanism of the SoxAX cytochromes has not been fully elucidated. Here we report the effects of several active site mutations on the spectroscopic and enzymatic properties of the type II SoxAX protein from Starkeya novella, which in addition to two heme groups also contains a C...
Source: Journal of Inorganic Biochemistry - April 14, 2016 Category: Biochemistry Authors: Kilmartin JR, Bernhardt PV, Dhouib R, Hanson GR, Riley MJ, Kappler U Tags: J Inorg Biochem Source Type: research