Fe(III) - Sulfide interaction in globins: Characterization and quest for a putative Fe(IV)-sulfide species.
Abstract The present study reports findings regarding the contrast between H2S interaction with bovine hemoglobin (Hb) and horse heart myoglobin (Mb), in terms of binding and dissociation kinetics, affinities, and mechanism. At pH9.5, oxidation of ferric-sulfide adducts in presence of no free sulfide, using hexachloroiridate as oxidant is examined using stopped-flow UV-vis, EPR, vibrational spectroscopy and mass spectrometry. Oxidation of the ferric-sulfide adduct in such conditions occurs with a putative unstable Fe(IV)-sulfide adduct as intermediate that finally leads to a paramagnetic ferric species with distin...
Source: Journal of Inorganic Biochemistry - November 10, 2017 Category: Biochemistry Authors: Mot AC, Bischin C, Damian G, Attia AAA, Gal E, Dina N, Leopold N, Silaghi-Dumitrescu R Tags: J Inorg Biochem Source Type: research

Dietary luteolin protects against HgCl2-induced renal injury via activation of Nrf2-mediated signaling in rat.
Abstract Luteolin (Lut) belongs to the flavonoid family with various beneficial bioactivities. Here, we investigated whether Lut attenuate mercuric chloride (HgCl2)-induced renal injury in rat. We found that oral gavage administration of Lut (80mg/kg) alleviated anemia and renal histology upon HgCl2 treatment (80mg/L). Lut also significantly reduced HgCl2-induced oxidative stress and inflammatory, presenting as the reduced malondialdehyde (MDA) formation, increased glutathione (GSH) level, and inhibited activation of nuclear factor kappa B (NF-κB). Moreover, Lut protected renal cells from HgCl2-induced apopt...
Source: Journal of Inorganic Biochemistry - November 9, 2017 Category: Biochemistry Authors: Tan X, Liu B, Lu J, Li S, Baiyun R, Lv Y, Lu Q, Zhang Z Tags: J Inorg Biochem Source Type: research

Roles of N- and C-terminal domains in the ligand-binding properties of cytoglobin.
In this study, we prepared human Cygb mutants (ΔC, ΔN, and ΔNC) with either one or both terminal domains truncated and investigated the enzymatic functions and structural features by spectroscopic methods. Evaluation of the superoxide-scavenging activity between Cygb variants showed that the ΔC and ΔNC mutants exhibited slightly higher activity involving superoxide scavenging as compared with wild-type Cygb. Subsequent experiments involving ligand titration, flash photolysis, and resonance Raman spectroscopic studies suggested that the truncation of the C- and N-terminal domains resulted in le...
Source: Journal of Inorganic Biochemistry - November 9, 2017 Category: Biochemistry Authors: Hanai S, Tsujino H, Yamashita T, Torii R, Sawai H, Shiro Y, Oohora K, Hayashi T, Uno T Tags: J Inorg Biochem Source Type: research

Improving the safety of metal-based drugs by tuning their metabolism with chemoprotective agents.
Abstract Metal-based drugs remain a tiny minority of all drugs that are on the market. The success story of the quintessential metal-based drug cisplatin (CP), which is intravenously administered to 70% of all cancer patients, however, demonstrates the inherent potential of metal-based drugs. A distinct disadvantage of CP is the dose-limiting severe toxic-side effects that it exerts in patients. To better understand the biomolecular basis for its toxicity, we employed a metallomics method to observe all platinum metabolites that are formed in blood plasma. These investigations revealed that a highly toxic CP-deriv...
Source: Journal of Inorganic Biochemistry - November 8, 2017 Category: Biochemistry Authors: Gailer J Tags: J Inorg Biochem Source Type: research

In vitro effects of benzimidazole/thioether-copper complexes with antitumor activity on human erythrocytes.
vera-Martínez AR Abstract Two cytotoxic copper(II) complexes with N-H and N-methylated benzimidazole-derived ligands (Cu-L(1) and Cu-L(1Me); L(1)=bis(2-methylbenzimidazolyl)(2-methylthioethyl)amine, L(1Me)=bis(1-methyl-2-methylbenzimidazolyl)(2-methylthioethyl)amine) were synthesized and exposed to human erythrocytes and molecular models of its membrane. The latter were bilayers built-up of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), classes of lipids present in the external and internal moieties of the human red cell membrane, respectively. Scanning electron micros...
Source: Journal of Inorganic Biochemistry - November 6, 2017 Category: Biochemistry Authors: Suwalsky M, Castillo I, Sánchez-Eguía BN, Gallardo MJ, Dukes N, Santiago-Osorio E, Aguiñiga I, Rivera-Martínez AR Tags: J Inorg Biochem Source Type: research

The influence of the Cys46/Cys55 disulfide bond on the redox and spectroscopic properties of human neuroglobin.
Abstract Neuroglobin is a monomeric globin containing a six-coordinate heme b, expressed in the nervous system, which exerts an important neuroprotective role. In the human protein (hNgb), Cys46 and Cys55 form an intramolecular disulfide bond under oxidizing conditions, whose cleavage induces a helix-to-strand rearrangement of the CD loop that strengthens the bond between the heme iron and the distal histidine. Hence, it is conceivable that the intramolecular disulfide bridge modulates the functionality of human neuroglobin by controlling exogenous ligand binding. In this work, we investigated the influence of the...
Source: Journal of Inorganic Biochemistry - October 16, 2017 Category: Biochemistry Authors: Bellei M, Bortolotti CA, Di Rocco G, Borsari M, Lancellotti L, Ranieri A, Sola M, Battistuzzi G Tags: J Inorg Biochem Source Type: research

Synergistic effect of copper and arsenic upon oxidative stress, inflammation and autophagy alterations in brain tissues of Gallus gallus.
Abstract Arsenic or copper is one of the most highly toxic pollution that can cause dysfunction to brains, however, the exact mechanism remains unclear. The aim of the study is to investigate the mechanisms of arsenic or/and copper-induced oxidative stress, inflammation and autophagy in chicken brains and elucidate the interactions between arsenic and copper. A total of 72 1-day-old Hy-line chickens were divided into four groups (18 chickens per group) treated with 30mg/kg arsenic trioxide (As2O3) or/and 300mg/kg copper sulfate (CuSO4) for 12weeks. Histological signs of inflammation were found in the cerebrum, cer...
Source: Journal of Inorganic Biochemistry - October 12, 2017 Category: Biochemistry Authors: Sun X, Li J, Zhao H, Wang Y, Liu J, Shao Y, Xue Y, Xing M Tags: J Inorg Biochem Source Type: research

Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine.
Abstract In this work we developed new antibacterial composite materials using polydopamine (PDA) to trigger the deposition of silver nanoparticles (AgNPs) onto calcium phosphates, namely octacalcium phosphate (OCP) and α-tricalcium phosphate (αTCP). Functionalization of OCP and αTCP with a self-polymerized polydopamine layer was obtained by soaking the calcium phosphates in dopamine solution. The PDA surface of functionalized calcium phosphates (OCPd and αTCPd) promoted the deposition of AgNPs by reducing silver ions when soaked in a silver nitrate solution. The amount of deposited AgNPs c...
Source: Journal of Inorganic Biochemistry - October 10, 2017 Category: Biochemistry Authors: Forte L, Torricelli P, Bonvicini F, Boanini E, Gentilomi GA, Lusvardi G, Della Bella E, Fini M, Vecchio Nepita E, Bigi A Tags: J Inorg Biochem Source Type: research

Synthesis, photophysical and cellular characterisation of folate and methotrexate labelled luminescent lanthanide complexes.
Abstract In this work we have developed a series of highly emissive europium(III) and terbium(III) complexes tethered to either folic acid (FA) or methotrexate (MTX), with the aim of developing visual probes that enable the imaging of folate receptors in cancer cells. The synthesis, photophysical properties and cellular behaviour are reported for four new lanthanide Ln(III) complexes, where either FA or MTX are tethered to 1,4,7-tris(carbonylmethyl)-10-(4'-quinolineacetic acid, (7'-acetamido)-1',2'-dihydro-2'-oxo)-1,4,7,10-tetraazacyclododecane Ln(III) complex, and Ln(III)=Eu(III) or Tb(III); herein referred to as...
Source: Journal of Inorganic Biochemistry - October 10, 2017 Category: Biochemistry Authors: Du Z, Sun J, Bader CA, Brooks DA, Li M, Li X, Plush SE Tags: J Inorg Biochem Source Type: research

Synthesis, structures, and ESI-mass studies of silver(I) derivatives of imidazolidine-2-thiones: Antimicrobial potential and biosafety evaluation.
Abstract The basic objective of this investigation is to explore potential metallo-organic antimicrobial agents based on silver-heterocyclic-2-thiones. In this respect, a series of silver(I) halide complexes with imidazolidine-2-thiones (L-NR, R=H, Me, Et, Pr(n), Bu(n), Ph), namely, mononuclear [AgX(L-NR)(PPh3)2] (X, R: Cl, Bu, 1; Br, Ph, 7); [AgX(L-NR)3] (Br, Bu, 5; Br, Pr(n), 8) and halogen bridged dinuclear [Ag2(μ-X)2(L-NR)2(PPh3)2] (Cl, Bu(n), 2; Cl, Ph, 3; Cl, Pr(n), 4; Br, Ph, 6) have been synthesized and characterized using modern techniques. The thio-ligands are terminally S-bonded in all the complexes....
Source: Journal of Inorganic Biochemistry - October 7, 2017 Category: Biochemistry Authors: Aulakh JK, Lobana TS, Sood H, Arora DS, Smolinski VA, Duff CE, Jasinski JP Tags: J Inorg Biochem Source Type: research

Coordination, redox properties and SOD activity of Cu(II) complexes of multihistidine peptides.
Várnagy K Abstract The results of electrochemical and SOD activity measurements of such copper(II) complexes of terminally protected multihistidine peptides that may mimic the active site of CuZnSOD enzyme are submitted and completed with solution equilibrium studies of some copper(II)-ligand systems. The equilibrium data confirm that the thermodynamic stabilities increase with the increasing number of histidyl residues in the amino acid sequence, the stability order, however, can be finely tuned by the number and quality of amino acids between histidine residues. Based on the cyclic voltammetric studies w...
Source: Journal of Inorganic Biochemistry - September 30, 2017 Category: Biochemistry Authors: Csire G, Timári S, Asztalos J, Király JM, Kiss M, Várnagy K Tags: J Inorg Biochem Source Type: research

Elucidation of one step synthesis of PEGylated CuFe bimetallic nanoparticles. Antimicrobial activity of CuFe@PEG vs Cu@PEG.
Abstract There is a growing field of research on the physicochemical properties of bimetallic nanoparticles (BMNPs) and their potential use in different applications. Meanwhile, their antimicrobial activity is scarcely reported, although BMNPs can potentially achieve unique chemical transformations and synergetic effects can be presented. Towards this direction a reproducible simple hybrid polyol process under moderate temperature solvothermal conditions has been applied for the isolation of non-oxide contaminated bimetallic CuFe nanoparticles (NPs). 1,2-propylene glycol (PG), tetraethylene glycol (TEG) and polyet...
Source: Journal of Inorganic Biochemistry - September 23, 2017 Category: Biochemistry Authors: Antonoglou O, Giannousi K, Arvanitidis J, Mourdikoudis S, Pantazaki A, Dendrinou-Samara C Tags: J Inorg Biochem Source Type: research

Synthesis, structural characterization and biological properties of phosphorescent iridium(III) complexes.
Abstract Two phosphorescent cyclometalated iridium(III)-triptycenyl-1,10-phenanthroline complexes [Ir(ppy)2(tpt-phen)](+) (1) and [Ir(bhq)2(tpt-phen)](+) (2) {ppy=2-phenylpyridine, bhq=Benzo[h]quinoline, tpt-phen=triptycenyl-1,10-phenanthroline} have been synthesized and structurally characterized. The structure of complex 2 has been studied by single crystal X-ray crystallography. The photophysical properties of complexes in a different solvent have also been investigated. The binding of complexes to the double stranded calf thymus (CT-DNA) has been investigated by spectroscopic techniques. These complexes conden...
Source: Journal of Inorganic Biochemistry - September 22, 2017 Category: Biochemistry Authors: Bhat SS, Shivalingegowda N, Revankar VK, Lokanath NK, Kugaji MS, Kumbar V, Bhat K Tags: J Inorg Biochem Source Type: research

Synthesis, crystal structure and antiproliferative mechanisms of 2-acetylpyridine-thiosemicarbazones Ga(III) with a greater selectivity against tumor cells.
Abstract Thiosemicarbazone Ga(III) complexes (C3-C5) were synthesized and characterized by X-ray single crystal diffraction, and they were all 1:1 ligand/Ga(III) complexes. The antiproliferative activity of these Ga(III) complexes was tested against three cancer cell lines, demonstrating that Ga(III) complexes showed about 3-10 folds more anticancer activity than their ligands alone. Importantly, thiosemicarbazones and Ga(III) complexes have a low toxicity to human fetal lung fibroblast cells (MRC-5) and exhibit a high therapeutic index for tumor cells. The results of UV-visible spectroscopy showed that the bindin...
Source: Journal of Inorganic Biochemistry - September 19, 2017 Category: Biochemistry Authors: Qi J, Zheng Y, Qian K, Tian L, Zhang GX, Cheng Z, Wang Y Tags: J Inorg Biochem Source Type: research

Novel coumarins and related copper complexes with biological activity: DNA binding, molecular docking and in vitro antiproliferative activity.
Abstract Coumarins show biological activity and are widely exploited for their therapeutic effects. Although a great number of coumarins substituted by heterocyclic moieties have been prepared, few studies have been carried out on coumarins containing pyridine heterocycle, which is known to modulate their physiological activities. We prepared and characterized three novel 3-(pyridin-2-yl)coumarins and their corresponding copper(II) complexes. We extended our investigations also to three known similar coumarins, since no data about their biochemical activity was previously been reported. The antiproliferative activ...
Source: Journal of Inorganic Biochemistry - September 18, 2017 Category: Biochemistry Authors: Pivetta T, Valletta E, Ferino G, Isaia F, Pani A, Vascellari S, Castellano C, Demartin F, Cabiddu MG, Cadoni E Tags: J Inorg Biochem Source Type: research

Protein oxidation involved in Cys-Tyr post-translational modification.
Abstract Some post-translationally modified tyrosines can perform reversible redox chemistry similar to metal cofactors. The most studied of these tyrosine modifications is the intramolecular thioether-crosslinked 3'-(S-cysteinyl)-tyrosine (Cys-Tyr) in galactose oxidase. This Cu-mediated tyrosine modification in galactose oxidase involves direct electron transfer (inner-sphere) to the coordinated tyrosine. Mammalian cysteine dioxygenase enzymes also contain a Cys-Tyr that is formed, presumably, through outer-sphere electron transfer from a non-heme iron center ~6Å away from the parent residues. An orphan pro...
Source: Journal of Inorganic Biochemistry - September 14, 2017 Category: Biochemistry Authors: Hromada SE, Hilbrands AM, Wolf EM, Ross JL, Hegg TR, Roth AG, Hollowell MT, Anderson CE, Benson DE Tags: J Inorg Biochem Source Type: research

Antitumor platinum(IV) derivatives of carboplatin and the histone deacetylase inhibitor 4-phenylbutyric acid.
Abstract Five new platinum(IV) derivatives of carboplatin each incorporating the histone deacetylase inhibitor 4-phenylbutyrate in axial position were synthesized and characterized by (1)H and (195)Pt NMR spectroscopy, electrospray ionization mass spectrometry and elemental analysis, namely cis,trans-[Pt(CBDCA)(NH3)2(PBA)(OH)] (1), cis,trans-[Pt(CBDCA)(NH3)2(PBA)2] (2), cis,trans-[Pt(CBDCA)(NH3)2(PBA)(bz)] (3), cis,trans-[Pt(CBDCA)(NH3)2(PBA)(suc)] (4) and cis,trans-[Pt(CBDCA)(NH3)2)(PBA)(ac)] (5) (PBA=4-phenylbutyrate, CBDCA=1,1-cyclobutane dicarboxylate, bz=benzoate, suc=succinate and ac=acetate). The reduction ...
Source: Journal of Inorganic Biochemistry - September 10, 2017 Category: Biochemistry Authors: Almotairy ARZ, Gandin V, Morrison L, Marzano C, Montagner D, Erxleben A Tags: J Inorg Biochem Source Type: research

Crystal structure of a novel RNA motif that allows for precise positioning of a single metal ion.
Abstract We have determined a crystal structure of an RNA duplex containing a novel metal-binding motif. The motif is composed of two sheared G○A base pairs, two unpaired A residues and four phosphate groups in close proximity. Four A residues make an A-A-A-A stacking column at the minor groove side and two G bases are highly inclined, thereby forming the pocket-shaped motif at the major groove side. In the present structure, a hydrated Sr(2+) ion exists in the pocket and binds to the O6 and N7 atoms of the two G bases and four phosphate groups. According to the previously-reported metal-binding properties to RN...
Source: Journal of Inorganic Biochemistry - September 9, 2017 Category: Biochemistry Authors: Kanazawa H, Kondo J Tags: J Inorg Biochem Source Type: research

A small-molecule acts as a 'roadblock' on DNA, hampering its fundamental processes.
Abstract DNA replication, RNA and protein synthesis are the most fundamental housekeeping processes involved in an organism's growth. Failure or dysregulation of these pathways are often deleterious to life. Therefore, selective inhibition of such processes can be crucial for the inhibition of the growth of any cell, including cancer cells, pathogenic bacteria or other deadly microbes. In the present study, a Zn(2+) complex is shown to act as a roadblock of DNA. The Zn(2+) complex inhibited DNA taq polymerase activity under the in vitro conditions of polymerase chain reaction (PCR). Under in vivo conditions, it re...
Source: Journal of Inorganic Biochemistry - September 6, 2017 Category: Biochemistry Authors: Kumar A Tags: J Inorg Biochem Source Type: research

J Inorg Biochem; +112 new citations
112 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: J Inorg Biochem These pubmed results were generated on 2017/09/05PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - September 6, 2017 Category: Biochemistry Tags: Report Source Type: research

Selective Ru(II)/lawsone complexes inhibiting tumor cell growth by apoptosis.
a AA Abstract New Ru(II) complexes with lawsone (law) characterized as trans-[Ru(law)(PPh3)2(N-N)]PF6, where PPh3 means triphenylphosphine and N-N is 2,2'-bipyridine (1), 4,4'-dimethyl-2,2'-bipyridine (2), 4,4'-dimethoxy-2,2'-bipyridine (3), 1,10-phenanthroline (4) or 4,7-diphenyl-1,10-phenanthroline (5), induce apoptosis in tumor cells. Cytotoxicity of the complexes against the tumor cell lines DU-145 (prostate cancer cells), MCF-7 (breast cancer cells), A549 (lung cancer cells) and lung non-tumor cell line MRC-5 demonstrated promising IC50 values, lower than those found for the cisplatin, a drug used as a refere...
Source: Journal of Inorganic Biochemistry - August 26, 2017 Category: Biochemistry Authors: Oliveira KM, Liany LD, Corrêa RS, Deflon VM, Cominetti MR, Batista AA Tags: J Inorg Biochem Source Type: research

Aluminum exposure for 60days at an equivalent human dietary level promotes peripheral dysfunction in rats.
rs GA Abstract Aluminum (Al) is a neurotoxic associated with a number of chronic human diseases. We investigated the effects of Al exposure at doses similar to human dietary levels and at a high level exposure to Al on the peripheral nervous system. Wistar male rats were divided into two major groups and received orally: 1) First group - Low level - rats were subdivided and treated for 60days: a) Control - received ultrapure water; b) AlCl3 - received Al at 8.3mg/kg body weight (bw) for 60days; and 2) Second group - High level - rats were subdivided and treated for 42days: C) Control - received ultrapure water thr...
Source: Journal of Inorganic Biochemistry - August 25, 2017 Category: Biochemistry Authors: Martinez CS, Vera G, Ocio JAU, Peçanha FM, Vassallo DV, Miguel M, Wiggers GA Tags: J Inorg Biochem Source Type: research

The crystal structures of a copper-bound metallochaperone from Saccharomyces cerevisiae.
Abstract Atx1 is a metallochaperone protein from the yeast Saccharomyces cerevisiae (yAtx1) that plays a major role in copper homeostasis in this organism. yAtx1 functions as a copper transfer protein by shuttling copper to the secretory pathway to control intracellular copper levels. Here we describe the first crystal structures of yAtx1 that have been determined in the presence of Cu(I). The structures from two different crystal forms have been solved and refined to resolutions of 1.65 and 1.93Å. In contrast to the previous metallated crystal structure of yAtx1 where a single Hg(II) atom was coordinated by...
Source: Journal of Inorganic Biochemistry - August 24, 2017 Category: Biochemistry Authors: Lee M, Cooray NDG, Maher MJ Tags: J Inorg Biochem Source Type: research

Influence of ancillary ligands on preferential geometry and biomimetic catalytic activity in manganese(III)-catecholate systems: A combined experimental and theoretical study.
A, Panja A Abstract The present report describes the synthesis and structural characterizations of six new manganese(III) complexes with redox-active tetrachlorocatecholate ligand in the presence of different ancillary ligands (pyridines and imidazole). X-ray crystal structure analysis reveals that the geometry of manganese(III) centres in 1 and 2 is essentially square pyramidal, while it is discrete octahedron in compounds 3-6. These preferential structural diversities in these systems have been critically analysed by theoretical calculations. Remarkably, the characterization of both π⋯π stacking interac...
Source: Journal of Inorganic Biochemistry - August 24, 2017 Category: Biochemistry Authors: Jana NC, Brandão P, Bauzá A, Frontera A, Panja A Tags: J Inorg Biochem Source Type: research

Molar absorption coefficients and stability constants of Zincon metal complexes for determination of metal ions and bioinorganic applications.
Abstract Zincon (ZI) is one of the most common chromophoric chelating probes for the determination of Zn(2+) and Cu(2+) ions. It is also known to bind other metal ions. However, literature data on its binding properties and molar absorption coefficients are rather poor, varying among publications or determined only in certain conditions. There are no systematic studies on Zn(2+) and Cu(2+) affinities towards ZI performed under various conditions. However, this widely commercially available and inexpensive agent is frequently the first choice probe for the measurement of metal binding and release as well as determi...
Source: Journal of Inorganic Biochemistry - August 24, 2017 Category: Biochemistry Authors: Kocyła A, Pomorski A, Krężel A Tags: J Inorg Biochem Source Type: research

An XAS investigation of the nickel site structure in the transcriptional regulator InrS.
Abstract InrS (Internal nickel-responsive Sensor) is a transcriptional repressor of the nickel exporter NrsD and de-represses expression of the exporter upon binding Ni(II) ions. Although a crystal structure of apo-InrS has been reported, no structure of the protein with metal ions bound is available. Herein we report the results of metal site structural investigations of Ni(II) and Cu(II) complexes of InrS using X-ray absorption spectroscopy (XAS) that are complementary to data available from the apo-InrS crystal structure, and are consistent with a planar four-coordinate [Ni(His)2(Cys)2] structure, where the lig...
Source: Journal of Inorganic Biochemistry - August 10, 2017 Category: Biochemistry Authors: Carr CE, Foster AW, Maroney MJ Tags: J Inorg Biochem Source Type: research

Synthesis, structural studies and biological activity of novel Cu(II) complexes with thiourea derivatives of 4-azatricyclo[5.2.1.0(2,6)]dec-8-ene-3,5-dione.
Abstract The new Cu(II) complexes of 1/2/3-(bromophenyl)-3-(1,7,8,9-tetramethyl-3,5-dioxo-4-azatricyclo[5.2.1.0(2,6)]dec-8-en-4-yl)thiourea derivatives have been synthesized. The spectroscopic studies together with density functional theory calculations of Cu(II) complexes revealed that two parent ligands coordinate to the copper cation in bidentate fashion via thiocarbonyl S and deprotonated N atoms forming rarely observed four-membered chelate ring, with nearly planar [CuN2S2] moiety. In solid state, the mononuclear complex is formed for thiourea derivative with 3-bromophenyl, whereas for Cu(II) connection with ...
Source: Journal of Inorganic Biochemistry - August 9, 2017 Category: Biochemistry Authors: Drzewiecka-Antonik A, Rejmak P, Klepka MT, Wolska A, Pietrzyk P, Stępień K, Sanna G, Struga M Tags: J Inorg Biochem Source Type: research

para-Sulfonatocalix[4]arene and polyamidoamine dendrimer nanocomplexes as delivery vehicles for a novel platinum anticancer agent.
Abstract Novel para-sulfonatocalix[4]arene (sCX[4]) and polyamidoamine (PAMAM) dendrimer nanocomplexes were evaluated as delivery vehicles for the platinum anticancer agent [(1,10-phenanthroline)(1S,2S-diaminocyclohexane)platinum(II)] chloride (PHENSS). Different ratios of sCX[4] to PHENSS were tested for their compatibility, with a ratio of 6:1 sCX[4]:PHENSS having the best solubility. The loading of sCX[4], and sCX[4]-bound PHENSS, onto three different generations of PAMAM dendrimers (G3.0-5.0) was examined using UV-visible spectrophotometry. The quantity of sCX[4] bound was found to increase exponentially with ...
Source: Journal of Inorganic Biochemistry - August 8, 2017 Category: Biochemistry Authors: Pang CT, Ammit AJ, Ong YQE, Wheate NJ Tags: J Inorg Biochem Source Type: research

Effects of the fluorine substituent positions of the intercalating ligands on the binding behavior and third-strand stabilization of two Ru(II) complexes toward poly(U) •poly(A)*poly(U) triplex RNA.
Effects of the fluorine substituent positions of the intercalating ligands on the binding behavior and third-strand stabilization of two Ru(II) complexes toward poly(U)•poly(A)*poly(U) triplex RNA. J Inorg Biochem. 2017 Aug 08;175:276-283 Authors: Peng M, Ni W, Tan L Abstract Two new Ru(II) polypyridyl complexes containing fluorine substituents, [Ru(bpy)2(o-fpip)](2+) (Ru1, bpy=2,2'-bipyridine, o-fpip=2-(2-fluorophenyl)imidazo[4,5-f] [1,10]phenanthroline) and [Ru(bpy)2(p-fpip)](2+) (Ru2, p-fpip=2-(4-fluorophenyl)imidazo[4,5-f] [1,10]phenanthroline) have been synthesized as binders for poly(U)&bul...
Source: Journal of Inorganic Biochemistry - August 8, 2017 Category: Biochemistry Authors: Peng M, Ni W, Tan L Tags: J Inorg Biochem Source Type: research

Reprint of "Anticancer activity of hydroxy- and sulfonamide-azobenzene platinum(II) complexes in cisplatin-resistant ovarian cancer cells".
Reprint of "Anticancer activity of hydroxy- and sulfonamide-azobenzene platinum(II) complexes in cisplatin-resistant ovarian cancer cells". J Inorg Biochem. 2017 Aug 08;: Authors: Samper KG, Marker SC, Bayón P, MacMillan SN, Keresztes I, Palacios Ò, Wilson JJ Abstract The syntheses of three platinum(II) complexes bearing sulfonamide-((E)-2-(4-methylphenylsulfonamido)-2',6'-difluoroazobenzene, HL1) and hydroxy-azo-2,6-difluorobenzene ((E)-2-((2,6-difluorophenyl)diazenyl)phenol, HL2) bidentate ligands is described. These complexes, [Pt(L1)(DMSO)Cl] (1), [Pt(L2)(DMSO)Cl] (2), and ...
Source: Journal of Inorganic Biochemistry - August 8, 2017 Category: Biochemistry Authors: Samper KG, Marker SC, Bayón P, MacMillan SN, Keresztes I, Palacios Ò, Wilson JJ Tags: J Inorg Biochem Source Type: research

Speciation of the trivalent f-elements Eu(III) and Cm(III) in digestive media.
This study, therefore, focuses on the chemical speciation of trivalent f-elements in the human gastrointestinal tract. To simulate the digestive system artificial digestive juices (saliva, gastric juice, pancreatic juice and bile fluid) were prepared. The chemical speciation of lanthanides (as Eu(III)) and actinides (as Cm(III)) was determined experimentally by time-resolved laser-induced fluorescence spectroscopy (TRLFS) and the results were compared with thermodynamic modeling. The results indicate a dominant inorganic species with phosphate/carbonate in the mouth, while the aquo ion is predominantly formed with a minor ...
Source: Journal of Inorganic Biochemistry - August 4, 2017 Category: Biochemistry Authors: Wilke C, Barkleit A, Stumpf T, Ikeda-Ohno A Tags: J Inorg Biochem Source Type: research

Dinuclear silver(I)-N-heterocyclic carbene complexes: Synthesis, characterization and larvicidal activity of bis-imidazolium dinuclear silver(I)-N-heterocyclic carbene complexes.
Abstract New synthesized bis-imidazolium salts that are linked by xylyl derivatives moiety, 1-4 was reacted with Ag2O to facilitate the formation of dinuclear Ag(I)-N-heterocyclic carbene (NHC) complexes, 5-8, respectively. All the synthesized ligand salts and complexes were characterized by (1)H and (13)C NMR, FTIR spectroscopy and elemental analysis. Molecular structures of compounds 3, 5, and 7 were elucidated by single crystal X-ray diffraction analyses. Larvicidal studies against the Aedes aegypti and Culex quinquefasciatus were carried out on all synthesized compounds following the World Health Organization ...
Source: Journal of Inorganic Biochemistry - August 4, 2017 Category: Biochemistry Authors: Selvarajoo PD, Haque RA, Haziz UFM, Avicor SW, Wajidi MFF, Razali MR Tags: J Inorg Biochem Source Type: research

Dimeric and trimeric homo- and heteroleptic hydroxamic acid macrocycles formed using mixed-ligand Fe(III)-based metal-templated synthesis.
Abstract Macrocyclic hydroxamic acids coordinate Fe(III) with high affinity as part of siderophore-mediated bacterial iron acquisition. Trimeric hydroxamic acid macrocycles, such as desferrioxamine E (DFOE), are prevalent in nature, with fewer dimeric macrocycles identified, including putrebactin (pbH2), avaroferrin (avH2), bisucaberin (bsH2) and alcaligin (alH2). This work used metal-templated synthesis (MTS) to pre-assemble complexes between one equivalent of Fe(III) and two equivalents of 4-((4-aminobutyl)(hydroxy)amino)-4-oxobutanoic acid (BBH) or 4-((5-aminopentyl)(hydroxy)amino)-4-oxobutanoic acid (PBH). Fol...
Source: Journal of Inorganic Biochemistry - August 1, 2017 Category: Biochemistry Authors: Sresutharsan A, Tieu W, Richardson-Sanchez T, Soe CZ, Codd R Tags: J Inorg Biochem Source Type: research

An essential role of N-terminal domain of copper chaperone in the enzymatic activation of Cu/Zn-superoxide dismutase.
Abstract Cu/Zn-superoxide dismutase (SOD1) is an enzyme that disproportionates superoxide anion into hydrogen peroxide and molecular oxygen. The enzymatic activity of SOD1 requires the binding of copper and zinc ions and also the formation of a conserved intramolecular disulfide bond. In a eukaryotic cell, a copper chaperone for SOD1 (CCS) has been known to supply a copper ion and also introduce the disulfide bond into SOD1; however, a mechanism controlling the CCS-dependent activation of SOD1 remains obscure. Here, we characterized CCS isolated from a human liver fluke, Clonorchis sinensis, and found that an N-te...
Source: Journal of Inorganic Biochemistry - July 31, 2017 Category: Biochemistry Authors: Fukuoka M, Tokuda E, Nakagome K, Wu Z, Nagano I, Furukawa Y Tags: J Inorg Biochem Source Type: research

Antiparasitic activity against trypanosomatid diseases and novel metal complexes derived from the first time characterized 5-phenyl-1,2,4-triazolo[1,5-a]pyrimidi-7(4H)-one.
o M, Isac-García J, Salas JM Abstract A serie of isostructural complexes with general formula [M(ftpO)2(H2O)4] have been obtained from reaction between the first time characterized triazolopyrimidine derivative 5-phenyl-1,2,4-triazolo[1,5-a]pyrimidi-7(4H)-one (HftpO) (1) and first row transition nitrates (M=Cu (2), Co (3), Ni (4) and Zn (5)). A copper complex with formula [Cu(HftpO)2(NO3)2(H2O)2]·H2O (6) was also isolated. HftpO and their metal complexes have been characterized by spectroscopic and thermal analysis and their crystal structures have been solved by X-ray diffraction methods. The isostr...
Source: Journal of Inorganic Biochemistry - July 29, 2017 Category: Biochemistry Authors: Méndez-Arriaga JM, Esteban-Parra GM, Juárez MJ, Rodríguez-Diéguez A, Sánchez-Moreno M, Isac-García J, Salas JM Tags: J Inorg Biochem Source Type: research

Varying the metal to ethacrynic acid ratio in ruthenium(ii)/osmium(ii)-p-cymene conjugates.
Abstract Following the identification of a ruthenium(II)-arene complex with an ethacrynic acid-modified imidazole ligand, which inhibits glutathione transferase (GST) and is cytotoxic to chemo-resistant cancer cells, a series of structurally related ruthenium(II)- and osmium(II)-p-cymene compounds have been prepared. In these complexes the ethacrynic acid is linked to the metals via appropriately modified pyridine ligands. The influence of the metal center and the metal:ethacrynic acid ratio on the cytotoxicity of the compounds was evaluated with the derivatives with one metal center and two ethacrynic acid moieti...
Source: Journal of Inorganic Biochemistry - July 28, 2017 Category: Biochemistry Authors: Păunescu E, Soudani M, Clavel CM, Dyson PJ Tags: J Inorg Biochem Source Type: research

Charge-transfer interactions of Cr species with DNA.
Abstract Interactions of Cr species with nucleic acids in living organisms depend strongly on Cr oxidation state and the environmental conditions. As the effects of these interactions range from benign to pre-mutagenic to carcinogenic, careful assessment of the hazard they pose to human health is necessary. We have investigated methods that would enable quantifying the DNA damage caused by Cr species under varying environmental conditions, including UV, O2, and redox potential, using simple instrumental techniques which could be in future combined into a field-deployable instrumentation. We have employed electroch...
Source: Journal of Inorganic Biochemistry - July 24, 2017 Category: Biochemistry Authors: Nowicka AM, Matysiak-Brynda E, Hepel M Tags: J Inorg Biochem Source Type: research

Aryl-1H-imidazole[4,5f][1,10]phenanthroline Cu(II) complexes: Electrochemical and DNA interaction studies.
Abstract The reaction of aryl imidazo[4,5f] [1,10]phenanthrolines with Cu(NO3)2 lead to the formation of Cu(II) complexes of the type [Cu(L)(NO3)2] where L=PIP, 2-(phenyl) [4,5f] imidazo phenanthroline; HPIP=2-(2-hydroxyphenyl)imidazo [4,5f] phenanthroline and NIP=2-(naphthyl) [4,5f] imidazo phenanthroline. The interaction of these complexes with calf thymus DNA has been studied using viscosity measurements, UV-visible and fluorescence spectroscopy. Chemical nuclease activity of these complexes has also been investigated. All complexes cleave DNA via oxidative pathway involving singlet oxygen. Molecular docking st...
Source: Journal of Inorganic Biochemistry - July 23, 2017 Category: Biochemistry Authors: Rajebhosale BS, Dongre SN, Deshpande SS, Kate AN, Kumbhar AA Tags: J Inorg Biochem Source Type: research

The influence of functional groups on the permeation and distribution of antimycobacterial rhodamine chelators.
Abstract We formerly hypothesized a mechanism whereby the antimycobacterial efficiency of a set of rhodamine labelled iron chelators is improved via the rhodamine fluorophore which enhances the chelators' permeation properties through membranes. To validate our hypothesis in a cellular context and to understand the influence of the structure of the fluorophore on the chelator's uptake and distribution within macrophages we now report comparative confocal microscopy studies performed with a set of rhodamine labelled chelators. We identify the functional groups of the chelator's framework that favor uptake by macrop...
Source: Journal of Inorganic Biochemistry - July 22, 2017 Category: Biochemistry Authors: Moniz T, Leite A, Silva T, Gameiro P, Gomes MS, de Castro B, Rangel M Tags: J Inorg Biochem Source Type: research

PML-RAR α stabilized by zinc in human acute promyelocytic leukemia NB4 cells.
PML-RARα stabilized by zinc in human acute promyelocytic leukemia NB4 cells. J Inorg Biochem. 2017 Jul 19;175:92-100 Authors: Zhu B, Wang JY, Zhou JJ, Zhou F, Cheng W, Liu YT, Wang J, Chen X, Chen DH, Luo L, Hua ZC Abstract Acute promyelocytic leukemia (APL) is characterized and driven by the promyelocytic leukemia protein-retinoic acid receptor alpha (PML-RARα) fusion gene. Previous studies have highlighted the importance of PML-RARα degradation in the treatment against APL. Considering the presence of two zinc fingers in the PML-RARα fusion protein, we explored the function o...
Source: Journal of Inorganic Biochemistry - July 19, 2017 Category: Biochemistry Authors: Zhu B, Wang JY, Zhou JJ, Zhou F, Cheng W, Liu YT, Wang J, Chen X, Chen DH, Luo L, Hua ZC Tags: J Inorg Biochem Source Type: research

Characterizing activation mechanisms and binding preferences of ruthenium metallo-prodrugs by a competitive binding assay.
Abstract The activation mechanisms and reactivity of ruthenium metallo-prodrug lead structures were investigated in detail using capillary zone electrophoresis mass spectrometry (CZE-MS) in a time-dependent manner and by exposing to a protein/oligonucleotide mixture. The competitive assays were performed with sodium trans-[RuCl4(HInd)2] where Hind=indazole (NKP-1339), [(η(6)-p-cymene)RuCl2(pta)], where pta=1,3,5-triaza-7-phosphaadamantane (RAPTA-C) and [(η(6)-biphenyl)RuCl(1,2-ethylenediamine)]PF6 (RM175). Molecular and quantitative information on binding preferences was obtained by coupling CZE to electro...
Source: Journal of Inorganic Biochemistry - July 16, 2017 Category: Biochemistry Authors: Artner C, Holtkamp HU, Hartinger CG, Meier-Menches SM Tags: J Inorg Biochem Source Type: research

New dinuclear palladium(II) complexes: Studies of the nucleophilic substitution reactions, DNA/BSA interactions and cytotoxic activity.
ić B Abstract Six new dinuclear Pd(II) complexes, [{Pd(2,2'-bipy)Cl}2(μ-pz)](ClO4)2 (Pd1), [{Pd(dach)Cl}2(μ-pz)](ClO4)2 (Pd2), [{Pd(en)Cl}2(μ-pz)](ClO4)2 (Pd3), [{Pd(2,2'-bipy)Cl}2(μ-4,4'-bipy)](ClO4)2 (Pd4), [{Pd(dach)Cl}2(μ-4,4'-bipy)](ClO4)2 (Pd5) and [{Pd(en)Cl}2(μ-4,4'-bipy)](ClO4)2 (Pd6) (where 2,2'-bipy=2,2'-bipyridyl, pz=pyrazine, dach=trans-(±)-1,2-diaminocyclohexane, en=ethylenediamine, 4,4'-bipy=4,4'-bipyridyl) have been synthesized and characterized by elemental microanalysis, IR, (1)H NMR and MALDI-TOF mass spectrometry. The pKa values of corresponding diaqua complexes were d...
Source: Journal of Inorganic Biochemistry - July 16, 2017 Category: Biochemistry Authors: Ćoćić D, Jovanović S, Nišavić M, Baskić D, Todorović D, Popović S, Bugarčić ŽD, Petrović B Tags: J Inorg Biochem Source Type: research

Synthesis and anti-diabetic activity of new N,N-dimethylphenylenediamine-derivatized nitrilotriacetic acid vanadyl complexes.
Abstract Vanadium compounds are promising anti-diabetic agents. However, reducing the metal toxicity while keeping/improving the hypoglycemic effect is still a big challenge towards the success of anti-diabetic vanadium drugs. To improve the therapeutic potency using the anti-oxidative strategy, we synthesized new N,N-dimethylphenylenediamine (DMPD)-derivatized nitrilotriacetic acid vanadyl complexes ([VO(dmada)]). The in vitro biological evaluations revealed that the DMPD-derivatized complexes showed improved antioxidant capacity and lowered cytotoxicity on HK-2 cells than bis(maltolato)oxidovanadium (IV) (BMOV)....
Source: Journal of Inorganic Biochemistry - July 8, 2017 Category: Biochemistry Authors: Niu X, Yang J, Yang X Tags: J Inorg Biochem Source Type: research

Biotin-Pt (IV)-indomethacin hybrid: A targeting anticancer prodrug providing enhanced cancer cellular uptake and reversing cisplatin resistance.
Abstract A Pt(IV) prodrug (2) composed of cancer-targeting biotin and nonsteroidal anti-inflammatory drug indomethacin in the axial positions of the six-coordinated octahedral geometry derived from cisplatin was developed, which could be highly accumulated in cancer cells more than normal ones and activated by endogenous reducing molecules to release cisplatin and indomethacin moieties simultaneously to inhibit tumor progression synergistically. In vitro assays revealed that 2 exhibited significantly selective inhibition to the tested cancer cell lines and sensitivity to cisplatin resistant cancer cells. Moreover,...
Source: Journal of Inorganic Biochemistry - July 4, 2017 Category: Biochemistry Authors: Hu W, Fang L, Hua W, Gou S Tags: J Inorg Biochem Source Type: research

Improve the anticancer potency of the platinum(II) complexes through functionalized leaving group.
Abstract Two platinum(II) complexes with 3,3-dimethoxycyclobutane-1,1-dicarboxylate as a leaving group were synthesized and spectrally characterized. In vitro cytotoxicity study on these complexes indicated that complex 2 showed considerable cytotoxicity against the tested cell lines. Notably, the higher antiproliferative activity of complex 2 relative to the corresponding parent compound [Pt(dach)(CBDCA)] demonstrated that the introduction of two methoxy groups in the 1,1-cyclobutanedicarboxylate (CBDCA) can improve the anticancer activity of the resulting platinum(II) complexes. Moreover, cellular accumulations ...
Source: Journal of Inorganic Biochemistry - July 2, 2017 Category: Biochemistry Authors: Zhao J, Wang D, Xu G, Gou S Tags: J Inorg Biochem Source Type: research

Roles of a mitochondrial AccSCO2 gene from Apis cerana cerana in oxidative stress responses.
Abstract In eukaryotes, cytochrome c oxidase (COX) is a multimeric protein complex that is the last enzyme in the respiratory electron transport chain of mitochondria. Syntheses of cytochrome c oxidase (SCO) proteins are copper-donor chaperones involved in metalation of the CuA redox center of COX. However, its other precise actions are not yet understood. Here, we report the characterization of AccSCO2 from Apis cerana cerana (Acc). Our data showed that AccSCO2 expression was induced by cold (4°C), CdCl2, HgCl2, ultraviolet (UV) light, and H2O2 and was inhibited by different pesticide treatments. In addition,...
Source: Journal of Inorganic Biochemistry - July 2, 2017 Category: Biochemistry Authors: Jia H, Ma M, Zhai N, Liu Z, Wang H, Guo X, Xu B Tags: J Inorg Biochem Source Type: research

Anti-cancer gold(I) phosphine complexes: Cyclic trimers and tetramers containing the P-Au-P moiety.
We report the application of cationic tri- and tetra-nuclear gold(I) phosphine complexes [Au3(μ-dppen)3]X3 and [Au4(μ-dppa)4]X4 (X=OTf, PF6) [OTf=trifluoromethanesulfonate, dppen=trans-1,2-bis(diphenylphosphino)ethene, dppa=bis(diphenylphosphino)acetylene] for cancer treatment. The results of cytotoxicity tests on four different cancer cells [prostate (DU145), cervical (HeLa), breast (MDAMB-231) and fibro sarcoma (HT1080)] indicate these complexes possess remarkable tumor cell growth inhibitory effects and high selectivity towards cancer cells. The anti-tumor mechanism of the tri- and tetra-nuclear gold(I) complexes ...
Source: Journal of Inorganic Biochemistry - June 28, 2017 Category: Biochemistry Authors: Reddy TS, Privér SH, Mirzadeh N, Bhargava SK Tags: J Inorg Biochem Source Type: research

Controlling gold nanoparticle seeded growth in thermophilic ferritin protein templates.
In this study, we explore whether using a gold complex with electrostatic complementarity to the anionic ferritin cavity can promote efficient seeded nanoparticle growth. We also compare wt AfFtn and a closed pore mutant AfFtn to explore whether the ferritin pores influence final AuNP size. PMID: 28683348 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - June 27, 2017 Category: Biochemistry Authors: Pulsipher KW, Honig S, Deng S, Dmochowski IJ Tags: J Inorg Biochem Source Type: research

High resolution crystal structure of a fluoride-inhibited organophosphate-degrading metallohydrolase.
Abstract Metal ion-dependent, organophosphate-degrading enzymes (OP hydrolases) have received increasing attention due to their ability to degrade and thus detoxify commonly used pesticides and nerve agents such as sarin and VX. These enzymes thus garner strong potential as bioremediators. The OP hydrolase from Agrobacterium radiobacter (OpdA) is one of the most efficient members of this group of enzymes. Previous studies have indicated that the choice of the hydrolysis-initiating nucleophile may depend on the pH of the reaction, with a metal ion-bridging hydroxide being preferred at lower pH (i.e. pH≤8.5), and...
Source: Journal of Inorganic Biochemistry - June 27, 2017 Category: Biochemistry Authors: Selleck C, Guddat LW, Ollis DL, Schenk G, Pedroso MM Tags: J Inorg Biochem Source Type: research

Mononuclear gold(III) complexes with phenanthroline ligands as efficient inhibitors of angiogenesis: A comparative study with auranofin and sunitinib.
an MI Abstract Gold(III) complexes with 1,7- and 4,7-phenanthroline ligands, [AuCl3(1,7-phen-κN7)] (1) and [AuCl3(4,7-phen-κN4)] (2) were synthesized and structurally characterized by spectroscopic (NMR, IR and UV-vis) and single-crystal X-ray diffraction techniques. In these complexes, 1,7- and 4,7-phenanthrolines are monodentatedly coordinated to the Au(III) ion through the N7 and N4 nitrogen atoms, respectively. In comparison to the clinically relevant anti-angiogenic compounds auranofin and sunitinib, gold(III)-phenanthroline complexes showed from 1.5- to 20-fold higher anti-angiogenic potential, a...
Source: Journal of Inorganic Biochemistry - June 27, 2017 Category: Biochemistry Authors: Pavic A, Glišić BĐ, Vojnovic S, Warżajtis B, Savić ND, Antić M, Radenković S, Janjić GV, Nikodinovic-Runic J, Rychlewska U, Djuran MI Tags: J Inorg Biochem Source Type: research