Redox potentials elucidate the electron transfer pathway of NAD < sup > + < /sup > -dependent formate dehydrogenases
J Inorg Biochem. 2024 Jan 20;253:112487. doi: 10.1016/j.jinorgbio.2024.112487. Online ahead of print.ABSTRACTMetal-dependent, nicotine adenine dinucleotide (NAD+)-dependent formate dehydrogenases (FDHs) are complex metalloenzymes coupling biochemical transformations through intricate electron transfer pathways. Rhodobacter capsulatus FDH is a model enzyme for understanding coupled catalysis, in that reversible CO2 reduction and formate oxidation are linked to a flavin mononuclotide (FMN)-bound diaphorase module via seven iron-sulfur (FeS) clusters as a dimer of heterotetramers. Catalysis occurs at a bis-metal-binding pteri...
Source: Journal of Inorganic Biochemistry - February 2, 2024 Category: Biochemistry Authors: Benjamin R Duffus Marcel Gauglitz Christian Teutloff Silke Leimk ühler Source Type: research
Determination of the role of specific amino acids in the binding of Zn(II), Ni(II), and Cu(II) to the active site of the M10 family metallopeptidase
J Inorg Biochem. 2024 Jan 27;253:112500. doi: 10.1016/j.jinorgbio.2024.112500. Online ahead of print.ABSTRACTMetallopeptidases are a group of metal-dependent enzymes that hydrolyze peptide bonds. These enzymes found in Streptococcus pneumoniae assist the pathogen in infecting the host by breaking down host tissues and extracellular matrix proteins. Considering metallopeptidases' significant role in bacterial virulence, inhibiting this enzyme represents a promising avenue for research. These enzymes are characterized by the presence of Zn(II) in the active site, proper coordination of which is essential for their catalytic ...
Source: Journal of Inorganic Biochemistry - February 1, 2024 Category: Biochemistry Authors: Paulina Potok Martyna Zawada S ławomir Potocki Source Type: research
Monomeric copper(II) complexes with unsymmetrical salen environment: Synthesis, characterization and study of biological activities
J Inorg Biochem. 2024 Jan 24;253:112497. doi: 10.1016/j.jinorgbio.2024.112497. Online ahead of print.ABSTRACTThree new ONNO-donor tetradentate unsymmetrical salen ligands were synthesized by using o-phenyl diamine with substituted salicylaldehydes followed by a two-step reaction methodology. These three ligands by reaction with Cu(OAc)2.4H2O produced three new monomeric Cu(II) complexes, [CuII(L1-3)] (1-3). Elemental analysis, IR, UV-vis, NMR, and HR-ESI-MS techniques were used to analyze and characterize all the synthesized ligands and their corresponding metal complexes. Molecular structures of 1-3 were confirmed by the ...
Source: Journal of Inorganic Biochemistry - January 30, 2024 Category: Biochemistry Authors: Deepika Mohapatra Sushree Aradhana Patra Pratikshya Das Pattanayak Gurunath Sahu Takahiro Sasamori Rupam Dinda Source Type: research
Evaluation of heteroscorpionate ligands as scaffolds for the generation of Ruthenium(II) metallodrugs in breast cancer therapy
J Inorg Biochem. 2024 Jan 18;253:112486. doi: 10.1016/j.jinorgbio.2024.112486. Online ahead of print.ABSTRACTThe modular synthesis of the heteroscorpionate core is explored as a tool for the rapid development of ruthenium-based therapeutic agents. Starting with a series of structurally diverse alcohol-NN ligands, a family of heteroscorpionate-based ruthenium derivatives was synthesized, characterized, and evaluated as an alternative to platinum therapy for breast cancer therapy. In vitro, the antitumoral activity of the novel derivatives was assessed in a series of breast cancer cell lines using UNICAM-1 and cisplatin as m...
Source: Journal of Inorganic Biochemistry - January 24, 2024 Category: Biochemistry Authors: Elena Dom ínguez-Jurado Consuelo Ripoll Agust ín Lara-Sánchez Alberto Oca ña I ñigo J Vitórica-Yrezábal Iv án Bravo Carlos Alonso-Moreno Source Type: research
In Memoriam - Ademir Neves *1951- †2022
J Inorg Biochem. 2024 Jan 18;252:112485. doi: 10.1016/j.jinorgbio.2024.112485. Online ahead of print.NO ABSTRACTPMID:38241908 | DOI:10.1016/j.jinorgbio.2024.112485 (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - January 19, 2024 Category: Biochemistry Authors: Rosely A Peralta Source Type: research
Hole-scavenging in photo-driven N < sub > 2 < /sub > reduction catalyzed by a CdS-nitrogenase MoFe protein biohybrid system
J Inorg Biochem. 2024 Jan 9;253:112484. doi: 10.1016/j.jinorgbio.2024.112484. Online ahead of print.ABSTRACTThe light-driven reduction of dinitrogen (N2) to ammonia (NH3) catalyzed by a cadmium sulfide (CdS) nanocrystal‑nitrogenase MoFe protein biohybrid is dependent on a range of different factors, including an appropriate hole-scavenging sacrificial electron donor (SED). Here, the impact of different SEDs on the overall rate of N2 reduction catalyzed by a CdS quantum dot (QD)-MoFe protein system was determined. The selection of SED was guided by several goals: (i) molecules with standard reduction potentials sufficient...
Source: Journal of Inorganic Biochemistry - January 14, 2024 Category: Biochemistry Authors: Andrew Clinger Zhi-Yong Yang Lauren M Pellows Paul King Florence Mus John W Peters Gordana Dukovic Lance C Seefeldt Source Type: research
JIB Ademir Neves Special Issue Preface/Editorial
J Inorg Biochem. 2024 Mar;252:112483. doi: 10.1016/j.jinorgbio.2024.112483. Epub 2024 Jan 10.NO ABSTRACTPMID:38219444 | DOI:10.1016/j.jinorgbio.2024.112483 (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - January 14, 2024 Category: Biochemistry Authors: Cl áudio N Verani Rosely A Peralta Source Type: research
Soluble meso and deuteroporphyrin analogs of the malaria pigment hematin anhydride
J Inorg Biochem. 2024 Mar;252:112470. doi: 10.1016/j.jinorgbio.2023.112470. Epub 2023 Dec 22.ABSTRACTTwo soluble heme analogs of the insoluble malaria pigment hematin anhydride (HA, or β-hematin), [Fe(III)(protoporphyrin)]2, with either mesoporphyrin (MHA) or deuteroporphyrin (DHA) are characterized by elemental analysis, SEM, IR spectroscopy, electronic spectroscopy, paramagnetic 1H NMR spectroscopy and solution magnetic susceptibility. While prior single crystal and X-ray powder diffraction results indicate all three have a common propionate linked dimer motif, there is considerable solid state variation in the conforma...
Source: Journal of Inorganic Biochemistry - January 13, 2024 Category: Biochemistry Authors: Liliana Su árez Aaron J Kosar Erin L Dodd Dagobert Tazoo Ambre Charlotte Lambert D Scott Bohle Source Type: research
Oxygen-selective regulation of cyclic di-GMP synthesis by a globin coupled sensor with a shortened linking domain modulates Shewanella sp. ANA-3 biofilm
J Inorg Biochem. 2024 Mar;252:112482. doi: 10.1016/j.jinorgbio.2024.112482. Epub 2024 Jan 8.ABSTRACTBacteria utilize heme proteins, such as globin coupled sensors (GCSs), to sense and respond to oxygen levels. GCSs are predicted in almost 2000 bacterial species and consist of a globin domain linked by a central domain to a variety of output domains, including diguanylate cyclase domains that synthesize c-di-GMP, a major regulator of biofilm formation. To investigate the effects of middle domain length and heme edge residues on GCS diguanylate cyclase activity and cellular function, a putative diguanylate cyclase-containing...
Source: Journal of Inorganic Biochemistry - January 13, 2024 Category: Biochemistry Authors: Ariel Schuelke-Sanchez Neela H Yennawar Emily E Weinert Source Type: research
Mercury binding to proteins disclosed by ESI MS experiments: The case of three organomercurials
J Inorg Biochem. 2024 Mar;252:112479. doi: 10.1016/j.jinorgbio.2024.112479. Epub 2024 Jan 6.ABSTRACTSolution interactions of three organomercury compounds, i.e., methylmercury chloride, thimerosal and phenylmercury acetate, with a group of biochemically relevant proteins, namely cytochrome c (Cyt c), ribonuclease A (RNase A), carbonic anhydrase I (hCA I), superoxide dismutase (SOD), and serum albumin (HSA), were investigated using an established ESI MS approach. Temporal analysis of sample aliquots provided insight into the binding kinetics, while comparative analysis of the obtained mass spectra disclosed adduct formation...
Source: Journal of Inorganic Biochemistry - January 13, 2024 Category: Biochemistry Authors: Andrea Geri Stefano Zineddu Lara Massai Luisa Ronga Ryszard Lobinski J ürgen Gailer Luigi Messori Source Type: research
Zinc complexes of chloroquine and hydroxychloroquine versus the mixtures of their components: Structures, solution equilibria/speciation and cellular zinc uptake
J Inorg Biochem. 2024 Mar;252:112478. doi: 10.1016/j.jinorgbio.2024.112478. Epub 2024 Jan 3.ABSTRACTThe zinc complexes of chloroquine (CQ; [Zn(CQH+)Cl3]) and hydroxychloroquine (HO-CQ; [Zn(HO-CQH+)Cl3]) were synthesized and characterized by X-Ray structure analysis, FT-IR, NMR, UV-Vis spectroscopy, and cryo-spray mass spectrometry in solid state as well as in aqueous and organic solvent solutions, respectively. In acetonitrile, up to two Zn2+ ions bind to CQ and HO-CQ through the tertiary amine and aromatic nitrogen atoms (KN-aminCQ = (3.8 ± 0.5) x 104 M-1 and KN-aromCQ = (9.0 ± 0.7) x 103 M-1 for CQ, and KN-aminHO-CQ = ...
Source: Journal of Inorganic Biochemistry - January 13, 2024 Category: Biochemistry Authors: Andrea Squarcina Alicja Franke Laura Senft Constantin Onderka Jens Langer Thibaut Vignane Milos R Filipovic Peter Grill Bernhard Michalke Ivana Ivanovi ć-Burmazović Source Type: research
N-heterocyclic-carbene vs diphosphine auxiliary ligands in thioamidato Cu(I) and Ag(I) complexes towards the development of potent and dual-activity antibacterial and apoptosis-inducing anticancer agents
J Inorg Biochem. 2024 Mar;252:112472. doi: 10.1016/j.jinorgbio.2023.112472. Epub 2023 Dec 23.ABSTRACTGroup 11 metal complexes exhibit promising antibacterial and anticancer properties which can be further enhanced by appropriate ligands. Herein, a series of mononuclear thioamidato Cu(I) and Ag(I) complexes bearing either a diphosphine (P^P) or a N-heterocyclic carbene (NHC) auxiliary ligand (L) was synthesized, and the impact of the co-ligand L on the in vitro antibacterial and anticancer properties of their complexes was assessed. All complexes effectively inhibited the growth of various bacterial strains, with the NHC-Cu...
Source: Journal of Inorganic Biochemistry - January 12, 2024 Category: Biochemistry Authors: Despoina Varna George Geromichalos Dimitra K Gioftsidou Demetrios Tzimopoulos Antonios G Hatzidimitriou Panagiotis Dalezis Rigini Papi Dimitrios Trafalis Panagiotis A Angaridis Source Type: research
Dinuclear copper(II) complexes with a bridging bis(chalcone) ligand reveal considerable in vitro cytotoxicity on human cancer cells and enhanced selectivity
J Inorg Biochem. 2024 Mar;252:112481. doi: 10.1016/j.jinorgbio.2024.112481. Epub 2024 Jan 4.ABSTRACTA bis(chalcone) molecule (H2L) was synthesized via Aldol's condensation from terephthalaldehyde and 2'-hydroxyacetophenone and it was used as bridging ligand for the preparation of five dinuclear copper(II) complexes of the composition [Cu(NN)(μ-L)Cu(NN)](NO3)2⋅nH2O (n = 0-2) (1-5), where NN stands for a bidentate N-donor ligand such as phen (1,10-phenanthroline, 1), bpy (2,2'-bipyridine, 2), mebpy (5,5'-dimethyl-2,2'-dipyridine, 3), bphen (bathophenanthroline, 4) and nphen (5-nitro-1,10-phenanthroline, 5). The compounds ...
Source: Journal of Inorganic Biochemistry - January 12, 2024 Category: Biochemistry Authors: Zden ěk Trávníček J án Vančo Jan Belza Giorgio Zoppellaro Zden ěk Dvořák Source Type: research
Antitumor active trans ‑platinum complexes through metabolic stability and enhanced cellular accumulation
J Inorg Biochem. 2024 Mar;252:112475. doi: 10.1016/j.jinorgbio.2023.112475. Epub 2023 Dec 29.ABSTRACTUtilizing isoquinoline as a carrier ligand, we have evaluated the reactivity of selected trans‑platinum planar amine (TPA) carboxylate compounds by varying the leaving carboxylate group (acetate, hydroxyacetate, and lactate) in an effort to optimize the cytotoxic and metabolic efficiency. To measure the pharmacological properties of these compounds, a combination of systematic biophysical and biological studies were carried out mainly involving substitution reaction with NAM (N-acetyl-methionine), effects on DNA structura...
Source: Journal of Inorganic Biochemistry - January 10, 2024 Category: Biochemistry Authors: Vijay Menon Samantha J Katner Daniel E Lee Erica J Peterson Jennifer E Koblinski Nicholas P Farrell Source Type: research
Decomposition of 2,4-dihalophenols by dehaloperoxidase activity and spontaneous reaction with hydrogen peroxide
J Inorg Biochem. 2024 Mar;252:112473. doi: 10.1016/j.jinorgbio.2023.112473. Epub 2023 Dec 24.ABSTRACTThe enzyme dehaloperoxidase (DHP) found in the marine worm Amphitrite ornata is capable of enzymatic peroxidation of 2,4-dichlorophenol (DCP) and 2,4-dibromophenol (DBP). There is also at least one parallel oxidative pathway and the major products 2-chloro-1,4-benzoquinone (2-ClQ) and 2-bromo-1,4-benzoquinone (2-BrQ) undergo aspontaneous secondary hydroxylation reaction. The oxidation and hydroxylation reactions have been monitored by UV-visible spectroscopy, High Performance Liquid Chromatography (HPLC), and mass spectrome...
Source: Journal of Inorganic Biochemistry - January 10, 2024 Category: Biochemistry Authors: Mst Sharmin Aktar Ransom Hill Wyatt Holbert Stefan Franzen Source Type: research
