-HH and -HAAAH motifs act as fishing nets for biologically relevant metal ions in metallopeptides
J Inorg Biochem. 2023 Dec 19;252:112456. doi: 10.1016/j.jinorgbio.2023.112456. Online ahead of print.ABSTRACTHistidine are one of the most common residues involved in transition metal ion binding in the active sites of metalloenzymes. In order to mimic enzymatic metal binding sites, it is crucial to understand the basic coordination modes of histidine residues, distributed at different positions in the peptide sequence. We show that: (i) the separation of two histidines has a large effect on complex stability - a sequence with adjusting histidine residues forms more stable complexes with Zn(II) than the one in which the re...
Source: Journal of Inorganic Biochemistry - December 28, 2023 Category: Biochemistry Authors: Aleksandra Hecel Kinga Garstka Henryk Koz łowski Magdalena Rowi ńska-Żyrek Source Type: research
-HH and -HAAAH motifs act as fishing nets for biologically relevant metal ions in metallopeptides
J Inorg Biochem. 2023 Dec 19;252:112456. doi: 10.1016/j.jinorgbio.2023.112456. Online ahead of print.ABSTRACTHistidine are one of the most common residues involved in transition metal ion binding in the active sites of metalloenzymes. In order to mimic enzymatic metal binding sites, it is crucial to understand the basic coordination modes of histidine residues, distributed at different positions in the peptide sequence. We show that: (i) the separation of two histidines has a large effect on complex stability - a sequence with adjusting histidine residues forms more stable complexes with Zn(II) than the one in which the re...
Source: Journal of Inorganic Biochemistry - December 28, 2023 Category: Biochemistry Authors: Aleksandra Hecel Kinga Garstka Henryk Koz łowski Magdalena Rowi ńska-Żyrek Source Type: research
-HH and -HAAAH motifs act as fishing nets for biologically relevant metal ions in metallopeptides
J Inorg Biochem. 2023 Dec 19;252:112456. doi: 10.1016/j.jinorgbio.2023.112456. Online ahead of print.ABSTRACTHistidine are one of the most common residues involved in transition metal ion binding in the active sites of metalloenzymes. In order to mimic enzymatic metal binding sites, it is crucial to understand the basic coordination modes of histidine residues, distributed at different positions in the peptide sequence. We show that: (i) the separation of two histidines has a large effect on complex stability - a sequence with adjusting histidine residues forms more stable complexes with Zn(II) than the one in which the re...
Source: Journal of Inorganic Biochemistry - December 28, 2023 Category: Biochemistry Authors: Aleksandra Hecel Kinga Garstka Henryk Koz łowski Magdalena Rowi ńska-Żyrek Source Type: research
-HH and -HAAAH motifs act as fishing nets for biologically relevant metal ions in metallopeptides
J Inorg Biochem. 2023 Dec 19;252:112456. doi: 10.1016/j.jinorgbio.2023.112456. Online ahead of print.ABSTRACTHistidine are one of the most common residues involved in transition metal ion binding in the active sites of metalloenzymes. In order to mimic enzymatic metal binding sites, it is crucial to understand the basic coordination modes of histidine residues, distributed at different positions in the peptide sequence. We show that: (i) the separation of two histidines has a large effect on complex stability - a sequence with adjusting histidine residues forms more stable complexes with Zn(II) than the one in which the re...
Source: Journal of Inorganic Biochemistry - December 28, 2023 Category: Biochemistry Authors: Aleksandra Hecel Kinga Garstka Henryk Koz łowski Magdalena Rowi ńska-Żyrek Source Type: research
-HH and -HAAAH motifs act as fishing nets for biologically relevant metal ions in metallopeptides
J Inorg Biochem. 2023 Dec 19;252:112456. doi: 10.1016/j.jinorgbio.2023.112456. Online ahead of print.ABSTRACTHistidine are one of the most common residues involved in transition metal ion binding in the active sites of metalloenzymes. In order to mimic enzymatic metal binding sites, it is crucial to understand the basic coordination modes of histidine residues, distributed at different positions in the peptide sequence. We show that: (i) the separation of two histidines has a large effect on complex stability - a sequence with adjusting histidine residues forms more stable complexes with Zn(II) than the one in which the re...
Source: Journal of Inorganic Biochemistry - December 28, 2023 Category: Biochemistry Authors: Aleksandra Hecel Kinga Garstka Henryk Koz łowski Magdalena Rowi ńska-Żyrek Source Type: research
Enhancing ferryl accumulation in H < sub > 2 < /sub > O < sub > 2 < /sub > -dependent cytochrome P450s
We report the characterization of a highly chemoselective CYP decarboxylase from Staphylococcus aureus (OleTSA) that is soluble at high concentrations. Examination of OleTSA Compound I (CpdI) accumulation with a variety of fatty acid substrates reveals a dependence on resting spin-state equilibrium. Alteration of this equilibrium through targeted mutagenesis of the proximal pocket favors the high-spin form, and as a result, enhances Cpd-I accumulation to nearly stoichiometric yields.PMID:38141432 | DOI:10.1016/j.jinorgbio.2023.112458 (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - December 23, 2023 Category: Biochemistry Authors: Jose A Amaya Olivia M Manley Julia C Bian Cooper D Rutland Nicholas Leschinsky Steven C Ratigan Thomas M Makris Source Type: research
Effects of removal of the axial methionine heme ligand on the binding of S. cerevisiae iso-1 cytochrome c to cardiolipin
J Inorg Biochem. 2023 Dec 18;252:112455. doi: 10.1016/j.jinorgbio.2023.112455. Online ahead of print.ABSTRACTThe cleavage of the axial S(Met) - Fe bond in cytochrome c (cytc) upon binding to cardiolipin (CL), a glycerophospholipid of the inner mitochondrial membrane, is one of the key molecular changes that impart cytc with (lipo)peroxidase activity essential to its pro-apoptotic function. In this work, UV - VIS, CD, MCD and fluorescence spectroscopies were used to address the role of the Fe - M80 bond in controlling the cytc-CL interaction, by studying the binding of the Met80Ala (M80A) variant of S. cerevisiae iso-1 cytc...
Source: Journal of Inorganic Biochemistry - December 23, 2023 Category: Biochemistry Authors: Alessandro Paradisi Marzia Bellei Carlo Augusto Bortolotti Giulia Di Rocco Antonio Ranieri Marco Borsari Marco Sola Gianantonio Battistuzzi Source Type: research
Enhancing ferryl accumulation in H < sub > 2 < /sub > O < sub > 2 < /sub > -dependent cytochrome P450s
We report the characterization of a highly chemoselective CYP decarboxylase from Staphylococcus aureus (OleTSA) that is soluble at high concentrations. Examination of OleTSA Compound I (CpdI) accumulation with a variety of fatty acid substrates reveals a dependence on resting spin-state equilibrium. Alteration of this equilibrium through targeted mutagenesis of the proximal pocket favors the high-spin form, and as a result, enhances Cpd-I accumulation to nearly stoichiometric yields.PMID:38141432 | DOI:10.1016/j.jinorgbio.2023.112458 (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - December 23, 2023 Category: Biochemistry Authors: Jose A Amaya Olivia M Manley Julia C Bian Cooper D Rutland Nicholas Leschinsky Steven C Ratigan Thomas M Makris Source Type: research
Effects of removal of the axial methionine heme ligand on the binding of S. cerevisiae iso-1 cytochrome c to cardiolipin
J Inorg Biochem. 2023 Dec 18;252:112455. doi: 10.1016/j.jinorgbio.2023.112455. Online ahead of print.ABSTRACTThe cleavage of the axial S(Met) - Fe bond in cytochrome c (cytc) upon binding to cardiolipin (CL), a glycerophospholipid of the inner mitochondrial membrane, is one of the key molecular changes that impart cytc with (lipo)peroxidase activity essential to its pro-apoptotic function. In this work, UV - VIS, CD, MCD and fluorescence spectroscopies were used to address the role of the Fe - M80 bond in controlling the cytc-CL interaction, by studying the binding of the Met80Ala (M80A) variant of S. cerevisiae iso-1 cytc...
Source: Journal of Inorganic Biochemistry - December 23, 2023 Category: Biochemistry Authors: Alessandro Paradisi Marzia Bellei Carlo Augusto Bortolotti Giulia Di Rocco Antonio Ranieri Marco Borsari Marco Sola Gianantonio Battistuzzi Source Type: research
Enhancing ferryl accumulation in H < sub > 2 < /sub > O < sub > 2 < /sub > -dependent cytochrome P450s
We report the characterization of a highly chemoselective CYP decarboxylase from Staphylococcus aureus (OleTSA) that is soluble at high concentrations. Examination of OleTSA Compound I (CpdI) accumulation with a variety of fatty acid substrates reveals a dependence on resting spin-state equilibrium. Alteration of this equilibrium through targeted mutagenesis of the proximal pocket favors the high-spin form, and as a result, enhances Cpd-I accumulation to nearly stoichiometric yields.PMID:38141432 | DOI:10.1016/j.jinorgbio.2023.112458 (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - December 23, 2023 Category: Biochemistry Authors: Jose A Amaya Olivia M Manley Julia C Bian Cooper D Rutland Nicholas Leschinsky Steven C Ratigan Thomas M Makris Source Type: research
Effects of removal of the axial methionine heme ligand on the binding of S. cerevisiae iso-1 cytochrome c to cardiolipin
J Inorg Biochem. 2023 Dec 18;252:112455. doi: 10.1016/j.jinorgbio.2023.112455. Online ahead of print.ABSTRACTThe cleavage of the axial S(Met) - Fe bond in cytochrome c (cytc) upon binding to cardiolipin (CL), a glycerophospholipid of the inner mitochondrial membrane, is one of the key molecular changes that impart cytc with (lipo)peroxidase activity essential to its pro-apoptotic function. In this work, UV - VIS, CD, MCD and fluorescence spectroscopies were used to address the role of the Fe - M80 bond in controlling the cytc-CL interaction, by studying the binding of the Met80Ala (M80A) variant of S. cerevisiae iso-1 cytc...
Source: Journal of Inorganic Biochemistry - December 23, 2023 Category: Biochemistry Authors: Alessandro Paradisi Marzia Bellei Carlo Augusto Bortolotti Giulia Di Rocco Antonio Ranieri Marco Borsari Marco Sola Gianantonio Battistuzzi Source Type: research
Enhancing ferryl accumulation in H < sub > 2 < /sub > O < sub > 2 < /sub > -dependent cytochrome P450s
We report the characterization of a highly chemoselective CYP decarboxylase from Staphylococcus aureus (OleTSA) that is soluble at high concentrations. Examination of OleTSA Compound I (CpdI) accumulation with a variety of fatty acid substrates reveals a dependence on resting spin-state equilibrium. Alteration of this equilibrium through targeted mutagenesis of the proximal pocket favors the high-spin form, and as a result, enhances Cpd-I accumulation to nearly stoichiometric yields.PMID:38141432 | DOI:10.1016/j.jinorgbio.2023.112458 (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - December 23, 2023 Category: Biochemistry Authors: Jose A Amaya Olivia M Manley Julia C Bian Cooper D Rutland Nicholas Leschinsky Steven C Ratigan Thomas M Makris Source Type: research
Effects of removal of the axial methionine heme ligand on the binding of S. cerevisiae iso-1 cytochrome c to cardiolipin
J Inorg Biochem. 2023 Dec 18;252:112455. doi: 10.1016/j.jinorgbio.2023.112455. Online ahead of print.ABSTRACTThe cleavage of the axial S(Met) - Fe bond in cytochrome c (cytc) upon binding to cardiolipin (CL), a glycerophospholipid of the inner mitochondrial membrane, is one of the key molecular changes that impart cytc with (lipo)peroxidase activity essential to its pro-apoptotic function. In this work, UV - VIS, CD, MCD and fluorescence spectroscopies were used to address the role of the Fe - M80 bond in controlling the cytc-CL interaction, by studying the binding of the Met80Ala (M80A) variant of S. cerevisiae iso-1 cytc...
Source: Journal of Inorganic Biochemistry - December 23, 2023 Category: Biochemistry Authors: Alessandro Paradisi Marzia Bellei Carlo Augusto Bortolotti Giulia Di Rocco Antonio Ranieri Marco Borsari Marco Sola Gianantonio Battistuzzi Source Type: research
A voltammetric peptide biosensor for Cu < sup > 2+ < /sup > metal ion quantification in coffee seeds
J Inorg Biochem. 2024 Feb;251:112441. doi: 10.1016/j.jinorgbio.2023.112441. Epub 2023 Nov 29.ABSTRACTA prion-derived copper(II)-binding peptide was assembled onto a gold electrode for the building of a voltammetric biosensor for measuring the Cu2+ metal ion in biological samples. The chosen sequence was H-CVNITKQHTVTTTT-NH2, with an appended cysteine residue for binding to the gold surface as a self-assembled monolayer and a histidine residue as the anchorage point for copper(II) complexation. The biosensor showed a linear range of 10-7 to 10-6 M with an 8.0 × 10-8 M detection limit and a 1.0 × 10-7 M quantification limi...
Source: Journal of Inorganic Biochemistry - December 16, 2023 Category: Biochemistry Authors: Gabriella Magarelli Jonatas Gomes da Silva Caroline Luchtenberg Ribeiro Thiago Viana de Freitas Magali Aparecida Rodrigues Eric de Souza Gil Pierre Marraccini Jurandir Rodrigues de Souza Clarissa Silva Pires de Castro Marcelo Porto Bemquerer Source Type: research
A voltammetric peptide biosensor for Cu < sup > 2+ < /sup > metal ion quantification in coffee seeds
J Inorg Biochem. 2023 Nov 29;251:112441. doi: 10.1016/j.jinorgbio.2023.112441. Online ahead of print.ABSTRACTA prion-derived copper(II)-binding peptide was assembled onto a gold electrode for the building of a voltammetric biosensor for measuring the Cu2+ metal ion in biological samples. The chosen sequence was H-CVNITKQHTVTTTT-NH2, with an appended cysteine residue for binding to the gold surface as a self-assembled monolayer and a histidine residue as the anchorage point for copper(II) complexation. The biosensor showed a linear range of 10-7 to 10-6 M with an 8.0 × 10-8 M detection limit and a 1.0 × 10-7 M quantificat...
Source: Journal of Inorganic Biochemistry - December 16, 2023 Category: Biochemistry Authors: Gabriella Magarelli Jonatas Gomes da Silva Caroline Luchtenberg Ribeiro Thiago Viana de Freitas Magali Aparecida Rodrigues Eric de Souza Gil Pierre Marraccini Jurandir Rodrigues de Souza Clarissa Silva Pires de Castro Marcelo Porto Bemquerer Source Type: research
