Copper ion vs copper metal-organic framework catalyzed NO release from bioavailable S-Nitrosoglutathione en route to biomedical applications: Direct 1H NMR monitoring in water allowing identification of the distinct, true reaction stoichiometries and thiol dependencies.
Abstract Copper containing compounds catalyze decomposition of S-Nitrosoglutathione (GSNO) in the presence of glutathione (GSH) yielding glutathione disulfide (GSSG) and nitric oxide (NO). Extended NO generation from an endogenous source is medically desirable to achieve vasodilation, reduction in biofilms on medical devices, and antibacterial activity. Homogeneous and heterogeneous copper species catalyze release of NO from endogenous GSNO. One heterogeneous catalyst used for GSNO decomposition in blood plasma is the metal-organic framework (MOF), H3[(Cu4Cl)3-(BTTri)8, H3BTTri = 1,3,5-tris(1H-1,2,3-triazol-5-...
Source: Journal of Inorganic Biochemistry - July 16, 2019 Category: Biochemistry Authors: Tuttle RR, Rubin HN, Rithner CD, Finke RG, Reynolds MM Tags: J Inorg Biochem Source Type: research

Biophysical approaches for the study of metal-protein interactions.
Abstract Protein-protein interactions play important roles for a variety of cell functions, often involving metal ions; in fact, metal-ion binding mediates and regulates the activity of a wide range of biomolecules. Enlightening all of the specific features of metal-protein and metal-mediated protein-protein interactions can be a very challenging task; a detailed knowledge of the thermodynamic and spectroscopic parameters and the structural changes of the protein is normally required. For this purpose, many experimental techniques are employed, embracing all fields of Analytical and Bioinorganic Chemistry. In addi...
Source: Journal of Inorganic Biochemistry - July 16, 2019 Category: Biochemistry Authors: Witkowska D, Rowińska-Żyrek M Tags: J Inorg Biochem Source Type: research

Pt-Fe ferrocenyl compounds with hydroxyquinoline ligands show selective cytotoxicity on highly proliferative cells.
ino D Abstract Searching for a more effective chemotherapy for the treatment of Human African trypanosomiasis, the disease caused by the parasite Trypanosoma brucei, and cancer, in the current work five new [PtII(L)(dppf)](PF6) compounds, with HL = 8-hydroxyquinoline derivatives and dppf = 1,1'-bis(diphenylphosphino)ferrocene, were synthesized and fully characterized. Crystal structures of three compounds were solved by XRD. The compounds displayed fairly good activity against bloodstream T. brucei, with IC50 values in the submicromolar range (IC50: 0.14-0.93 μM), and good selectivity towards the path...
Source: Journal of Inorganic Biochemistry - July 16, 2019 Category: Biochemistry Authors: Rivas F, Medeiros A, Comini M, Suescun L, Rodríguez Arce E, Martins M, Pinheiro T, Marques F, Gambino D Tags: J Inorg Biochem Source Type: research

Potential role of serotonin as a biological reductant associated with copper transportation.
Abstract Serotonin (5-HT) is a neurotransmitter that is derived from tryptophan. Owing to a hydroxyl group attached to the indole nucleus, 5-HT exhibits a considerably higher redox activity than tryptophan. To gain insight into the biological relevance of the redox activity of 5-HT, the effect of Cu(I)-binding ligands on the 5-HT-mediated copper reduction was investigated. The d-d transition band of Cu(II) complexed with glycine [Cu(II)-Gly2] was not affected by addition of 5-HT alone but was diminished when a thioether-containing compound coexists with 5-HT. Concomitant with disappearance of the d-d transition ba...
Source: Journal of Inorganic Biochemistry - July 11, 2019 Category: Biochemistry Authors: Saito K, Watanabe K, Yanaoka R, Kageyama L, Miura T Tags: J Inorg Biochem Source Type: research

Modulation of ruthenium anticancer drugs analogs with tolfenamic acid: Reactivity, biological interactions and growth inhibition of yeast cell.
Abstract We synthesized two ruthenium(II) complexes: trans,trans-[Ru(im)2(tfa)2] (1) and trans,trans‑[Ru(in)2(tfa)2] (2) where im = 1H‑imidazole, in = 1H‑indazole and tfa = tolfenamic acid, a potential nonsteroidal anti-inflammatory drug (NSAID). The NSAID was opted as bioactive ligand to understand its synergistic therapeutic effect in structurally analogous Ru(II)-compounds with KP418 (imidazolium trans‑[tetrachloridobis(1H‑imidazole)ruthenate(III)]) and KP1019 (indazolium trans‑[tetrachloridobis(1H‑indazole)ruthenate(III)]). The complexes were studied using various analytical methods a...
Source: Journal of Inorganic Biochemistry - July 11, 2019 Category: Biochemistry Authors: Kumar P, Swagatika S, Dasari S, Tomar RS, Patra AK Tags: J Inorg Biochem Source Type: research

Redox thermodynamics of B-class dye-decolorizing peroxidases.
r C Abstract With>5000 annotated genes dye-decolorizing peroxidases (DyPs) represent a heme b peroxidase family of broad functional diversity. Bacterial B-class DyPs are poor peroxidases of unknown physiological function. Hydrogen peroxide efficiently mediates the rapid formation of Compound I in B-class DyPs, which, however, is stable and shows modest reactivity towards organic and inorganic electron donors. To understand these characteristics, we have investigated the redox thermodynamics of the one-electron reduction of the ferric high-spin form of wild-type B-class DyP from the pathogenic bacterium Klebsiel...
Source: Journal of Inorganic Biochemistry - July 11, 2019 Category: Biochemistry Authors: Pfanzagl V, Bellei M, Hofbauer S, Laurent CVFP, Furtmüller PG, Oostenbrink C, Battistuzzi G, Obinger C Tags: J Inorg Biochem Source Type: research

The copper(II) binding centres of carbonic anhydrase are differently affected by reductants that ensure the redox intracellular environment.
izzarelli E Abstract Copper is involved in several biological processes. The static and labile copper pools are controlled by means of a network of influx and efflux transporters, storage proteins, chaperones, transcription factors and small molecules as glutathione (GSH), which contributes to the cell reducing environment. To follow the fate of intracellular copper labile pool, a variant of human apocarbonic anhydrase has been proposed as fluorescent probe to monitor cytoplasmic Cu2+. Aware that in this cellular compartment copper ion is present as Cu+, electron spin resonance technique (ESR) was used to ascertai...
Source: Journal of Inorganic Biochemistry - July 3, 2019 Category: Biochemistry Authors: Tabbì G, Magrì A, Rizzarelli E Tags: J Inorg Biochem Source Type: research

Pd(II) complexes with N-heteroaromatic hydrazone ligands: Anticancer activity, in silico and experimental target identification.
lipović NR Abstract Anticancer activity of Pd complexes 1-5 with bidentate N-heteroaromatic hydrazone ligands was investigated on human acute monocytic leukemia (THP-1; cells in a suspension) and human mammary adenocarcinoma (MCF-7; two-dimensional layer and three-dimensional spheroid tumor model) cell lines. For the Pd(II) complexes with condensation products of ethyl hydrazainoacetate and quinoline-8-carboxaldehyde (complex 1) and 2-formylpyridine (complex 3), for which apoptosis was determined as a mechanism of anticancer activity, further investigation revealed that they arrest the cell cycle in G0/G1 phase, ...
Source: Journal of Inorganic Biochemistry - June 26, 2019 Category: Biochemistry Authors: Bjelogrlić SK, Todorović TR, Kojić M, Senćanski M, Nikolić M, Višnjevac A, Araškov J, Miljković M, Muller CD, Filipović NR Tags: J Inorg Biochem Source Type: research

New mononuclear and binuclear oxomolybdenum(V) complexes containing NN chelator: Syntheses, DFT calculations, interaction with BSA protein and in vitro cytotoxic activity.
Abstract A neutral bidentate ligand 2-(3-methyl-5-phenyl pyrazol-1-yl) benzthiazole (L) has been synthesized by refluxing equimolar proportions of 2-hydrazino benzthiazole and benzoyl acetone in ethanol. The ligand acts in a NN donor fashion and forms stable mononuclear, MoOX3L [L = Ligand, X = Cl (1), Br (2)] and binuclear Mo2O4X2L2 [L = Ligand, X = Cl (3), Br (4)] complexes with molybdenum(V). The ligand and complexes are thoroughly characterized by elemental analyses, IR, UV-Vis spectroscopy, EPR study, magnetic susceptibility, thermogravimetry and cyclic voltammetry. Magnetic moment measurement...
Source: Journal of Inorganic Biochemistry - June 25, 2019 Category: Biochemistry Authors: Roy M, Biswal D, Sarkar O, Pramanik NR, Drew MGB, Sadhukhan P, Kundu M, Sil PC, Chakrabarti S Tags: J Inorg Biochem Source Type: research

Evaluation of 5-hydroxy-1,4-naphthoquinone-cobalt(III) complexes for hypoxia-activated drug delivery.
anznaster M Abstract Three novel Py2N2-cobalt(III) complexes with the 5-hydroxy-1,4-naphthoquinone nuclei (NQ) were evaluated as potential hypoxia-activated anticancer prodrugs. The complexes were synthesized and fully characterized by IR and UV-Visible spectroscopies, ESI mass spectrometry and CHN elemental analysis. Structural information was obtained from density functional theory (DFT) calculations. Cyclic voltammetry analysis in acetonitrile indicates that the ligand substituents (H, CH3 and p-tolylthio) do not have a relevant effect on the Co3+/Co2+ redox potential. Reactions with ascorbic acid in phosphate ...
Source: Journal of Inorganic Biochemistry - June 25, 2019 Category: Biochemistry Authors: de Mello MVP, Cebrián-Torrejón G, Pereira JR, Dos Santos Moreira C, Gomes CBSMR, da Rocha DR, de Souza Fagundes EM, Ferreira GB, Lanznaster M Tags: J Inorg Biochem Source Type: research

Systematic investigation of the antiproliferative activity of a series of ruthenium terpyridine complexes.
Abstract Due to acquired resistance or limitations of the currently approved drugs against cancer, there is an urgent need for the development of new classes of compounds. Among others, there is an increasing attention towards the use of Ru(II) polypyridyl complexes. Most studies in the literature were made on complexes based on the coordination of N-donating bidentate ligands to the ruthenium core whereas studies on 2,2':6', 2″-terpyridine (terpy) coordinating ligands are relatively scare. However, several studies have shown that [Ru(terpy)2]2+ derivatives are able bind to DNA through various binding modes ...
Source: Journal of Inorganic Biochemistry - June 17, 2019 Category: Biochemistry Authors: Karges J, Blacque O, Jakubaszek M, Goud B, Goldner P, Gasser G Tags: J Inorg Biochem Source Type: research

The solution thermodynamic stability of desferrioxamine B (DFO) with Zr(IV).
Abstract Desferrioxamine B (DFO, [H4L]+, ligand) is currently the preferred chelator for 89Zr(IV), however the biological studies suggest that it releases the metal ion in vivo. Herein, we present the solution thermodynamics of complexes formed between Zr(IV) and this hexadentate chelating agent, the data surprisingly not yet available in the literature. Several techniques including electrospray ionization mass spectrometry (ESI-MS), potentiometry, UV-Vis spectroscopy and isothermal titration calorimetry (ITC) were used to determine the stoichiometry and thermodynamic stability of complexes formed in solution over...
Source: Journal of Inorganic Biochemistry - June 15, 2019 Category: Biochemistry Authors: Toporivska Y, Gumienna-Kontecka E Tags: J Inorg Biochem Source Type: research

Ru(II) complexes containing uracil nucleobase analogs with cytotoxicity against tumor cells.
We report on chemistry and cytotoxic studies of four new ruthenium (II) complexes containing uracil derivatives. All compounds are neutral, presenting the formula [Ru(PPh3)2(2TU)2] (1), [Ru(PPh3)2(6m2TU)2] (2), [Ru(dppb)(2TU)2] (3) and [Ru(dppb)(6m2TU)2] (4), where PPh3 = triphenylphosphine; dppb = 1,4-bis(diphenylphosphino)butane, 2TU = 2-thiouracil and 6m2TU = 6-methyl-2-thiouracil. They were characterized using NMR, UV-vis and IR spectroscopies, microanalytical analysis and mass spectrometry. Furthermore, the crystal structures of 1-4 were determined by single-crystal X-ray diffraction. The coordination ...
Source: Journal of Inorganic Biochemistry - June 9, 2019 Category: Biochemistry Authors: Correa RS, Bomfim LM, Oliveira KM, Moreira DRM, Soares MBP, Ellena J, Bezerra DP, Batista AA Tags: J Inorg Biochem Source Type: research

Bi- and trinuclear copper(I) compounds of 2,2,5,5-tetramethyl-imidazolidine-4-thione and 1,2-bis(diphenylphosphano)ethane: Synthesis, crystal structures, in vitro and in silico study of antibacterial activity and interaction with DNA and albumins.
Abstract Herein we report on the synthesis, molecular structures, DNA-binding properties and antibacterial activity of four new copper(I) mixed-ligand complexes obtained by reacting copper(I) halides or [Cu(CH3CN)4](BF4) with 1,2-bis(diphenylphosphano)ethane (dppe) and 2,2,5,5-tetramethylimidazolidine-4-thione (tmimdtH). Depending on the nature of the halide, the resulting compounds adopt two different structural motifs. Thus, using CuCl or CuBr, doubly dppe-bridged symmetrical dimmers of type [(κ-S-tmimdtH)XCu(μ-dppe)2CuX(κ-S-tmimdtH)] are formed, while in the case of CuI, a rare example of a trinu...
Source: Journal of Inorganic Biochemistry - June 8, 2019 Category: Biochemistry Authors: Anastasiadou D, Psomas G, Kalogiannis S, Geromichalos G, Hatzidimitriou AG, Aslanidis P Tags: J Inorg Biochem Source Type: research

DNA-binding and in vitro cytotoxic activity of platinum(II) complexes of curcumin and caffeine.
na MM, Mastrorilli P, Gamez P Abstract Three Pt(II) complexes containing the natural ligands curcumin and caffeine, namely [Pt(curc)(PPh3)2]Cl (1), [PtCl(curc)(DMSO)] (2) (curc = deprotonated curcumin) and trans-[Pt(caffeine)Cl2(DMSO)] (3), were synthesized and fully characterized. The data obtained suggest that, for both 1 and 2, the anion of curcumin is coordinated to the platinum ion via the oxygen atoms of the β-diketonate moiety. Spectroscopic features reveal that in 2 and 3, a DMSO molecule is S-bonded to the metal centre. For 3, all data indicate a square-planar geometry formed by a 9-N bonded caff...
Source: Journal of Inorganic Biochemistry - June 7, 2019 Category: Biochemistry Authors: Censi V, Caballero AB, Pérez-Hernández M, Soto-Cerrato V, Korrodi-Gregório L, Pérez-Tomás R, Dell'Anna MM, Mastrorilli P, Gamez P Tags: J Inorg Biochem Source Type: research

Exploring the cytotoxic activity of new phenanthroline salicylaldimine Zn(II) complexes.
Abstract Zinc(II) complexes bearing N-salicylideneglycinate (Sal-Gly) and 1,10-phenanthroline (phen) or phenanthroline derivatives [NN = 5-chloro-1,10-phenanthroline, 5-amine-1,10-phenanthroline (amphen), 4,7-diphenyl-1,10-phenanthroline (Bphen) and 5,6-epoxy-5,6-dihydro-1,10-phenanthroline] are synthesized. Complexes formulated as [Zn(NN)2(H2O)2]2+(NN = phen and amphen), are also prepared. The cytotoxicity of the compounds is evaluated towards a panel of human cancer cells: ovarian (A2780), breast (MCF7) and cervical (HeLa), as well as non-tumoral V79 fibroblasts. All compounds display higher cytotoxicity...
Source: Journal of Inorganic Biochemistry - June 1, 2019 Category: Biochemistry Authors: Matos CP, Addis Y, Nunes P, Barroso S, Alho I, Martins M, Matos APA, Marques F, Cavaco I, Costa Pessoa J, Correia I Tags: J Inorg Biochem Source Type: research

Stability of antibacterial Te(IV) compounds: A combined experimental and computational study.
Abstract Inorganic Te(IV) compounds are important cysteine protease inhibitors and antimicrobial agents; AS-101 [ammonium trichloro (dioxoethylene-O,O')tellurate] is the first compound of a family with formula NH4[C2H4Cl3O2Te], where a Te(IV) centre is bound to a chelate ethylene glycol, and showed several protective therapeutic applications. This compound is lacking in stability performance and is subjected to hydrolysis reaction with displacement of the diol ligand. In this paper, we report the stability trend of a series of analogues complexes of AS-101 with generic formula NH4[(RC2H3O2)Cl3Te], where R is an al...
Source: Journal of Inorganic Biochemistry - May 28, 2019 Category: Biochemistry Authors: D'Arcy K, Doyle AP, Kavanagh K, Ronconi L, Fresch B, Montagner D Tags: J Inorg Biochem Source Type: research

The electrochemical profiles of Auranofin and Aubipyc, two representative medicinal gold compounds: A comparative study.
lak K Abstract A micro-electrochemical reaction cell was coupled to an electrospray mass spectrometer in order to track redox transformations for two representative medicinal gold compounds - i.e. [(2,3,4,6-tetra-O-acetyl-1-thio-β-D-glucopyranosato-S)(triethylphosphine)gold(I)] and [Au(bipydmb-H)(OH)][PF6] (where bipydmb-H is deprotonated 6-(1,1-dimethylbenzyl)-2,2'-bipyridine), known as Auranofin and Aubipyc respectively - in parallel to square wave voltammetry (SWV) measurements. Irreversible oxidation of thio-glucose tetraacetate was the dominant reaction for the gold(I) compound Auranofin; oxidation was a...
Source: Journal of Inorganic Biochemistry - May 28, 2019 Category: Biochemistry Authors: Kupiec M, Ziółkowski R, Massai L, Messori L, Pawlak K Tags: J Inorg Biochem Source Type: research

Towards the functional high-resolution coordination chemistry of blood plasma human serum albumin.
Abstract Human serum albumin (HSA) is a monomeric, globular, multi-carrier and the most abundant protein in the blood. HSA displays multiple ligand binding sites with extraordinary binding capacity for a wide range of ions and molecules. For decades, HSA's ability to bind to various ligands has led many scientists to study its physiological properties and protein structure; indeed, a better understanding of HSA-ligand interactions in human blood, at the atomic level, will likely foster the development of more potent, and overall more performant, diagnostic and therapeutic tools against serious human disorders such...
Source: Journal of Inorganic Biochemistry - May 20, 2019 Category: Biochemistry Authors: Al-Harthi S, Lachowicz JI, Nowakowski ME, Jaremko M, Jaremko Ł Tags: J Inorg Biochem Source Type: research

Determination of the magnetic properties and orientation of the heme axial ligands of PpcA from Geobacter metallireducens by paramagnetic NMR.
Abstract The rising interest in the use of Geobacter bacteria for biotechnological applications demands a deep understanding of how these bacteria are able to thrive in a variety of environments and perform extracellular electron transfer. The Geobacter metallireducens bacterium can couple the oxidation of a wide range of compounds to the reduction of several extracellular acceptors, including heavy metals, toxic organic compounds or electrode surfaces. The periplasmic c-type cytochrome PpcA from this bacterium is a member of a family composed of five periplasmic triheme cytochromes, which are important to bridge ...
Source: Journal of Inorganic Biochemistry - May 17, 2019 Category: Biochemistry Authors: Fernandes TM, Morgado L, Salgueiro CA, Turner DL Tags: J Inorg Biochem Source Type: research

Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays.
ré A Abstract The influence of three functionalized hexavanadates (V6): Na2 [V6O13{(OCH2)3CCH3}2], [H2]2 [V6O13{(OCH2)3CCH2OCOCH2CH3}2] and [(C4H9)4N]2 [V6O13{(OCH2)3CCH2OOC(CH3)2-COOH}2 on Na+/K+-ATPase activity, was investigated in vitro. Including compounds already tested by Xu et al. (Journal of Inorganic Biochemistry 161 (2016) 27-36), all functionalized hexavanadates inhibit the activity of Na+/K+-ATPase in a dose-dependent manner but with different inhibitory potencies. Na2 [V6O13{(OCH2)3CCH3}2] was found to have the best inhibition properties - showing 50% inhibition IC50 = 5.50 × 10-5...
Source: Journal of Inorganic Biochemistry - May 16, 2019 Category: Biochemistry Authors: Bošnjaković-Pavlović N, Xu X, Krstić D, Gillet JM, Wei Y, Wu P, Čolović M, Spasojević-de Biré A Tags: J Inorg Biochem Source Type: research

Inhibitory effects of oxidovanadium complexes on the aggregation of human islet amyloid polypeptide and its fragments.
Abstract Human islet amyloid polypeptide (hIAPP) is synthesized by pancreatic β-cells and co-secreted with insulin. Misfolding and amyloidosis of hIAPP induce β-cell dysfunction in type II diabetes mellitus. Numerous small organic molecules and metal complexes act as inhibitors against amyloid-related diseases, justifying the need to explore the inhibitory mechanism of these compounds. In this work, three oxidovanadium complexes, namely, (NH4)[VO(O2)2(bipy)]·4H2O (1) (bipy = 2,2' bipyridine), bis(ethyl-maltolato, O,O)oxido-vanadium(IV) (2), and (bipyH2)H2[O{VO(O2)(bipy)}2]·5H2O (3), wer...
Source: Journal of Inorganic Biochemistry - May 16, 2019 Category: Biochemistry Authors: Xu J, Zhang B, Gong G, Huang X, Du W Tags: J Inorg Biochem Source Type: research

Novel binuclear and trinuclear metal (II) complexes: DNA interactions and in vitro anticancer activity through apoptosis.
Abstract A water soluble trinuclear copper(II) complex and a binuclear cobalt(II) complex, namely Cu3(ppbm)2(SO4)3 (1) and Co2(ppbm)2(NO3)4 (2) (ppbm = 2-(pyridin-2-yl)-1-(pyridin-3-ylmethyl)- 1H-benzo[d]imidazole), have been successfully synthesized and characterized by elemental analysis, IR Spectroscopy, electrospray ionization mass spectra (ESI-MS). The interaction of the new complexes with DNA has been explored using spectroscopy methods, indicating that the complexes 1 and 2 bind to DNA via noncovalent interactions. DNA cleavage experiment suggested that the complex 1 exhibits efficient DNA cleavage acti...
Source: Journal of Inorganic Biochemistry - May 10, 2019 Category: Biochemistry Authors: Zhao Z, Zhang J, Zhi S, Song W, Zhao J Tags: J Inorg Biochem Source Type: research

Effect of the point mutation H54N on the ferroxidase process of Rana catesbeiana H' ferritin.
Abstract Human H and Rana catesbeiana H' subunits in vertebrate ferritin protein cages catalyze the Fe(II) oxidation by molecular oxygen and promote the ferric oxide biomineral synthesis. By depositing iron biomineral, ferritins also prevent potentially toxic reactions products from Fe(II)-based Fenton chemistry. Recent work from our laboratory was aimed to describe the iron pathways within ferritin, from entrance into the cage to the ferroxidase site, and to understand the role played by amino-acid residues in iron trafficking and catalysis. Our approach exploits anomalous X-ray diffraction from ferritin crystals...
Source: Journal of Inorganic Biochemistry - May 7, 2019 Category: Biochemistry Authors: Pozzi C, Di Pisa F, Lalli D, Rosa C, Turano P, Mangani S Tags: J Inorg Biochem Source Type: research

Binding propterties of two Ru(II) polypyridyl complexes containing dppz units and fluorine groups with poly(U) ·poly(A) ∗ poly(U) triplex.
Binding propterties of two Ru(II) polypyridyl complexes containing dppz units and fluorine groups with poly(U)·poly(A) ∗ poly(U) triplex. J Inorg Biochem. 2019 May 02;197:110705 Authors: Wang F, Ma S, Feng Y, Liu X, Tan L Abstract In this work, two Ru(II)-dppz (dppz = dipyrido[3,2-a:2',3'-c]phenazine) complexes containing fluorine substituents, [Ru(bpy)2(7-F-dppz)]2+ (Ru1, bpy = 2,2'-bipyridine, 7-F-dppz = 7-fluorodipyrido[3,2-a:2',3'-c]phenazine) and [Ru(phen)2(7-F-dppz)]2+ (Ru2, phen = 1,10-phenanthroline), have been synthesized and characterized. Binding properties...
Source: Journal of Inorganic Biochemistry - May 2, 2019 Category: Biochemistry Authors: Wang F, Ma S, Feng Y, Liu X, Tan L Tags: J Inorg Biochem Source Type: research

Synthesis of a novel platinum(II) complex with 6,7-dichloro-5,8-quinolinedione and the study of its antitumor mechanism in testicular seminoma.
In conclusion, we observed that complex 1 can regulate the viability of testicular seminoma cells through the PI3K/Akt/GSK3β signaling pathway and the mitochondria-mediated apoptotic pathway in vitro, and thus, complex 1 may have potential for use as a drug in the treatment of testicular germ cell tumors. PMID: 31055215 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - April 27, 2019 Category: Biochemistry Authors: Li Z, Zhou J, Gan Y, Yin Y, Zhang W, Yang J, Tang Y, Dai Y Tags: J Inorg Biochem Source Type: research

The role of catalases in the prevention/promotion of oxidative stress.
Abstract Catalases, heme enzymes which catalyze decomposition of hydrogen peroxide to water and molecular oxygen, are important members of the antioxidant defense system of cells of almost all aerobic organisms. However, recent studies suggest that catalase may be involved in various other processes in the cell. The paper provides a review of reactions of catalases with their main substrate, hydrogen peroxide, and with oxidizing species such as hydroxyl radical, superoxide, nitric oxide, peroxynitrite, hypochlorous acid, and singlet oxygen. A number of these individuals are formed under oxidative eustress (good st...
Source: Journal of Inorganic Biochemistry - April 26, 2019 Category: Biochemistry Authors: Gebicka L, Krych-Madej J Tags: J Inorg Biochem Source Type: research

Melatonin protects against chromium(VI)-induced cardiac injury via activating the AMPK/Nrf2 pathway.
Abstract Chromium (Cr) threatens health by causing oxidative stress. However, effective therapy for cardiac damage mediated by potassium dichromate (K2Cr2O7) still has not been defined. Melatonin (MT) possesses a number of biological activities. Our study was performed to explore the effect and mechanism of MT on Cr(VI)-induced cardiac damage by conducting both in vitro and in vivo studies. Twenty eight male Wistar rats were randomly assigned to four groups: control, MT (20 mg/kg subcutaneously), K2Cr2O7 (4 mg/kg intraperitoneally), and K2Cr2O7 + MT. We measured biomarkers of oxidative stress and cardiac f...
Source: Journal of Inorganic Biochemistry - April 25, 2019 Category: Biochemistry Authors: Li J, Zheng X, Ma X, Xu X, Du Y, Lv Q, Li X, Wu Y, Sun H, Yu L, Zhang Z Tags: J Inorg Biochem Source Type: research

Azaindoles: Suitable ligands of cytotoxic transition metal complexes.
;vníček Z Abstract This minireview is devoted to the complexes of various transition metals, which contain azaindole ring coordinated to the metal centre, and whose cytotoxicity was studied. We decided to overview this interesting group of coordination compounds with the aim to highlight various structural types of complexes depending on the metal centre (i.e., Pt, Pd, Ru, Ir or Au) and type of the used co-ligand(s). The presented complexes are also reviewed in context of their toxicity, selectivity and processes connected with their mechanism of action. Some of complexes were also studied on in vivo model...
Source: Journal of Inorganic Biochemistry - April 24, 2019 Category: Biochemistry Authors: Štarha P, Trávníček Z Tags: J Inorg Biochem Source Type: research

Direct electrochemical reduction of carbon dioxide by a molybdenum-containing formate dehydrogenase.
a JJG Abstract Formate dehydrogenase enzymes catalyse the reversible two-electron oxidation of formate to carbon dioxide. The class of metal-dependent formate dehydrogenases comprises prokaryotic enzymes holding redox-active centres and a catalytic site, containing either molybdenum or tungsten ion, that mediates the formate/carbon dioxide interconversion. The carbon dioxide reduction is of a particular interest, since it may be a route for its atmospheric mitigation with the simultaneous production of added-value products, as formate-derived compounds. Recently, the periplasmic formate dehydrogenase from Desulfov...
Source: Journal of Inorganic Biochemistry - April 13, 2019 Category: Biochemistry Authors: Cordas CM, Campaniço M, Baptista R, Maia LB, Moura I, Moura JJG Tags: J Inorg Biochem Source Type: research

Metal-dependent interactions of metallothionein-3 β-domain with amyloid-β peptide and related physiological implications.
Metal-dependent interactions of metallothionein-3 β-domain with amyloid-β peptide and related physiological implications. J Inorg Biochem. 2019 Apr 13;196:110693 Authors: Jiang Z, Shen B, Xiang J Abstract Aberrant interactions of metal ions with amyloid-β peptide (Aβ) can potentiate Alzheimer's disease (AD) by participating in the aggregation process of Aβ and in the generation of reactive oxygen species (ROS). Metallothionein-3 (MT3), which is aberrantly expressed in AD brains, is believed to play an important role in the AD progression due to its ability of maintaining metal...
Source: Journal of Inorganic Biochemistry - April 13, 2019 Category: Biochemistry Authors: Jiang Z, Shen B, Xiang J Tags: J Inorg Biochem Source Type: research

Corrigendum to "Nuclear targeting terpyridine iron(II) complexes for cellular imaging and remarkable photocytotoxicity" [J. Inorg. Biochem. 116 (2012) 77-87].
Corrigendum to "Nuclear targeting terpyridine iron(II) complexes for cellular imaging and remarkable photocytotoxicity" [J. Inorg. Biochem. 116 (2012) 77-87]. J Inorg Biochem. 2019 Apr 13;:110692 Authors: Basu U, Khan I, Koley D, Saha S, Kondaiah P, Chakravarty AR PMID: 30992146 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - April 13, 2019 Category: Biochemistry Authors: Basu U, Khan I, Koley D, Saha S, Kondaiah P, Chakravarty AR Tags: J Inorg Biochem Source Type: research

Mannich base Cu(II) complexes as biomimetic oxidative catalyst.
Abstract Galactose Oxidase (GOase) and catechol oxidase (COase) are the metalloenzymes of copper having monomeric and dimeric sites of coordination, respectively. This paper summarizes the results of our studies on the structural, spectral and catalytic properties of new mononuclear copper (II) complexes [CuL(OAc)] (1), and [CuL2] (2), (HL = 2,4‑dichloro‑6‑{[(2'‑dimethyl‑aminoethyl)methylamino]methyl}‑phenol) which can mimic the functionalities of the metalloenzymes GOase and COase. The structure of the compounds has been elucidated by X-ray crystallography and the mimicked Cu(II) catalysts were fu...
Source: Journal of Inorganic Biochemistry - March 30, 2019 Category: Biochemistry Authors: Kundu BK, Ranjan R, Mukherjee A, Mobin SM, Mukhopadhyay S Tags: J Inorg Biochem Source Type: research

Design, synthesis and biological evaluation of cobalt(II)-Schiff base complexes as ATP-noncompetitive MEK1 inhibitors.
In this report, we designed and synthesized a series of cobalt(II)-Schiff base complexes (CoSBC) with competent MEK1 (mitogen-activated protein kinase kinase-1) inhibitory activity. Based on our previous report, the CoSBC exhibited high binding affinity with MEK1 protein. To further explore metal complexes as MEK1 inhibitors, a series of transition metals and ligands were employed to build a library of various metal Schiff base complexes. The MEK inhibition assays revealed that only CoSBC exhibited obvious inhibitory activity, complex 2b showed the best inhibition both in BRaf (B-rapidly accelerated fibrosarcoma)/MEK1 and ...
Source: Journal of Inorganic Biochemistry - March 30, 2019 Category: Biochemistry Authors: Li H, Xi D, Niu Y, Wang C, Xu F, Liang L, Xu P Tags: J Inorg Biochem Source Type: research

Silver(I) complexes with 4,7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection.
an MI Abstract Five novel silver(I) complexes with 4,7-phenanthroline (4,7-phen), [Ag(NO3-O)(4,7-phen-μ-N4,N7)]n (1), [Ag(ClO4-О)(4,7-phen-μ-N4,N7)]n (2), [Ag(CF3COO-O)(4,7-phen-μ-N4,N7)]n (3), [Ag2(H2O)0.58(4,7-phen)3](SbF6)2 (4) and {[Ag2(H2O)(4,7-phen-μ-N4,N7)2](BF4)2}n (5) were synthesized, structurally elucidated and biologically evaluated. These complexes showed selectivity towards Candida spp. in comparison to the tested bacteria and effectively inhibited the growth of four different Candida species, particularly of C. albicans strains, with minimal inhibitory concentrations (MICs) in the range...
Source: Journal of Inorganic Biochemistry - March 23, 2019 Category: Biochemistry Authors: Pavic A, Savić ND, Glišić BĐ, Crochet A, Vojnovic S, Kurutos A, Stanković DM, Fromm KM, Nikodinovic-Runic J, Djuran MI Tags: J Inorg Biochem Source Type: research

Insights on alpha lipoic and dihydrolipoic acids as promising scavengers of oxidative stress and possible chelators in mercury toxicology.
olo S Abstract Alpha lipoic acid (α-LA) and its reduced form dihydrolipoic acid (DHLA) have been historically considered as excellent anti-oxidants and oxidative stress scavengers. Upon oxidation with reactive oxygen species (ROS) and pro-oxidants, α-LA may be reconstituted from DHLA and other reduced forms. Oxidative stress is one of the fundamental causes of functional degeneration, autophagy and apoptosis leading to cytotoxicity and loss of cell survival, often due to exposure to xenobiotics, pollutants, heavy metals, and other environmental and endogenous toxicants. α-LA and DHLA can react wi...
Source: Journal of Inorganic Biochemistry - March 23, 2019 Category: Biochemistry Authors: Bjørklund G, Aaseth J, Crisponi G, Rahman MM, Chirumbolo S Tags: J Inorg Biochem Source Type: research

The essential metals for humans: a brief overview.
Abstract The human body needs about 20 essential elements in order to function properly and among them, for certain, 10 are metal elements, though for every metal we do need, there is another one in our body we could do without it. Until about 1950 poor attention was given to the so-called "inorganic elements" and while researches on "organic elements" (C, N, O and H) and organic compounds were given high priority, studies on essential inorganic elements were left aside. Base on current knowledge it is ascertained today that metals such as Na, K, Mg, Ca, Fe, Mn, Co, Cu, Zn and Mo are essential ...
Source: Journal of Inorganic Biochemistry - March 22, 2019 Category: Biochemistry Authors: Zoroddu MA, Aaseth J, Crisponi G, Medici S, Peana M, Nurchi VM Tags: J Inorg Biochem Source Type: research

Zinc-oxaprozin compounds: Synthesis, structure and biological activity.
Abstract Four novel zinc complexes, namely [Zn(oxa)2(MeOH)4] (1), [Zn(oxa)2(H2O)(bipy)]·MeOH·2.5H2O (2·MeOH·2.5H2O), [Zn(oxa)2(bipyam)]·1.25MeOH (3·1.25MeOH) and [Zn(oxa)2(phen)] (4), with the non-steroidal anti-inflammatory drug oxaprozin (Hoxa) and a N,N'-donor heterocyclic ligand, such as 2,2'‑bipyridylamine (bipyam), 1,10‑phenanthroline (phen) or 2,2'‑bipyridine (bipy), were characterized with physicochemical techniques, various spectroscopies and single-crystal X-ray crystallography. In these coordination compounds, the oxaprozin ligands are coordinated to zinc ...
Source: Journal of Inorganic Biochemistry - March 21, 2019 Category: Biochemistry Authors: Lazou M, Hatzidimitriou AG, Papadopoulos AN, Psomas G Tags: J Inorg Biochem Source Type: research

Antigiardiasic activity of Cu(II) coordination compounds: Redox imbalance and membrane damage after a short exposure time.
We present the antigiardiasic effect of eight Cu(II) coordination compounds, which belong to the family Casiopeínas. Two of them, 4,7-diphenyl-1,10-phenanthroline(acetylacetonato)copper(II) nitrate (CasIII-Ha,36 μM) and 4,7-diphenyl-1,10-phenanthroline(glycinato)copper(II) nitrate (CasI-gly,36 μM) have shown the best antiproliferative effect in Giardia intestinalis trophozoite cultures, both with the higher lipophilic character of the series. The antiproliferative effect of these coordination compounds is attributable to its capacity to interact with the cellular membrane and to increase reactive oxygen sp...
Source: Journal of Inorganic Biochemistry - March 21, 2019 Category: Biochemistry Authors: Rufino-González Y, Ponce-Macotela M, García-Ramos JC, Martínez-Gordillo MN, Galindo-Murillo R, González-Maciel A, Reynoso-Robles R, Tovar-Tovar A, Flores-Alamo M, Toledano-Magaña Y, Ruiz-Azuara L Tags: J Inorg Biochem Source Type: research

The hydrogen bonding network of coproheme in coproheme decarboxylase from Listeria monocytogenes: Effect on structure and catalysis.
h G Abstract Coproheme decarboxylase (ChdC) catalyzes the oxidative decarboxylation of coproheme to heme b, i.e. the last step in the recently described coproporphyrin-dependent pathway. Coproheme decarboxylation from Listeria monocytogenes is a robust enzymatic reaction of low catalytic efficiency. Coproheme acts as both substrate and redox cofactor activated by H2O2. It fully depends on the catalytic Y147 close to the propionyl group at position 2. In the present study we have investigated the effect of disruption of the comprehensive and conserved hydrogen bonding network between the four propionates and heme c...
Source: Journal of Inorganic Biochemistry - March 21, 2019 Category: Biochemistry Authors: Milazzo L, Gabler T, Pfanzagl V, Michlits H, Furtmüller PG, Obinger C, Hofbauer S, Smulevich G Tags: J Inorg Biochem Source Type: research

Phleomycin complex - Coordination mode and in vitro cleavage of DNA.
Abstract Phleomycin is one of the anticancer glycopeptide antibiotics which cause DNA cleavage. It is commonly used as a copper(II) complex. Therefore, it is important to study the metal ion binding process and to define the coordination mode. In this paper, we describe the acid-base properties of phleomycin and the coordination sphere of the Cu(II) cation. In the metal binding process up to five nitrogen donor atoms can be involved. Four of them in the same plane deriving from: the pyrimidine ring, secondary amine of β-aminoalanine, imidazole and amide of the nearest peptide bond (from β-hydroxyhistidin...
Source: Journal of Inorganic Biochemistry - March 21, 2019 Category: Biochemistry Authors: Stokowa-Sołtys K, Dzyhovskyi V, Wieczorek R, Jeżowska-Bojczuk M Tags: J Inorg Biochem Source Type: research

Studying the reactivity of "old" Cu(II) complexes for "novel" anticancer purposes.
Studying the reactivity of "old" Cu(II) complexes for "novel" anticancer purposes. J Inorg Biochem. 2019 Mar 18;195:51-60 Authors: Peña Q, Lorenzo J, Sciortino G, Rodríguez-Calado S, Maréchal JD, Bayón P, Simaan AJ, Iranzo O, Capdevila M, Palacios Ò Abstract Reactive oxygen species (ROS) formation appears as one of the most promising pathways to induce cell death. The interesting Cu(II)/Cu(I) redox pair has been reported to biologically generate ROS and induce cell damage. Simple metal complexes, such as cisplatin, sometimes offer even better prop...
Source: Journal of Inorganic Biochemistry - March 18, 2019 Category: Biochemistry Authors: Peña Q, Lorenzo J, Sciortino G, Rodríguez-Calado S, Maréchal JD, Bayón P, Simaan AJ, Iranzo O, Capdevila M, Palacios Ò Tags: J Inorg Biochem Source Type: research

Structures and anticancer activity of chlorido platinum(II) saccharinate complexes with mono- and dialkylphenylphosphines.
Abstract cis-[PtCl(sac)(PPh2Me)2] (1), cis-[PtCl(sac)(PPhMe2)2] (2), trans-[PtCl(sac)(PPh2Et)2] (3) and trans-[PtCl(sac)(PPhEt2)2] (4) complexes (sac = saccharinate) were synthesized and characterized by elemental analysis and spectroscopic methods. The structures of 2-4 were determined by X-ray single-crystal diffraction. The interaction of the complexes with DNA was studied various biochemical, biophysical and molecular docking methods. Only the cis-configured complexes (1 and 2) showed nuclease activity and their binding affinity towards DNA was considerably higher than those of their trans-congeners (3 and...
Source: Journal of Inorganic Biochemistry - March 14, 2019 Category: Biochemistry Authors: Icsel C, Yilmaz VT, Cevatemre B, Aygun M, Ulukaya E Tags: J Inorg Biochem Source Type: research

Structural analysis of copper(I) interaction with amyloid β peptide.
In this study we have analyzed Cu(I) interaction with the Amyloid β fragment encompassing the first 16 amino-acids. Our data were obtained by means of NMR spectroscopy which provided relevant structural details of the metal complexes. Our findings are consistent with the involvement of two or three His in the Cu(I) coordination sphere and indicate that His6 effectively participates to the metal binding. PMID: 30884319 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - March 7, 2019 Category: Biochemistry Authors: De Gregorio G, Biasotto F, Hecel A, Luczkowski M, Kozlowski H, Valensin D Tags: J Inorg Biochem Source Type: research

Increased ROS generation causes apoptosis-like death: Mechanistic insights into the anti-Leishmania activity of a potent ruthenium(II) complex.
We report, for the first time, that hmxbato and precursor promoted an increase in reactive oxygen species production, depolarization of the mitochondrial membrane, DNA fragmentation, formation of a pre-apoptotic peak, alterations in parasite morphology and formation of autophagic vacuoles. Taken together, our results suggest that these ruthenium complexes cause parasite death by apoptosis. Thus, this work provides relevant knowledge on the activity of ruthenium(II) complexes against L. (L.) amazonensis. Such information will be essential for the exploitation of these complexes as future candidates for cutaneous leishmanias...
Source: Journal of Inorganic Biochemistry - March 6, 2019 Category: Biochemistry Authors: Costa MS, Gonçalves YG, Teixeira SC, Nunes DCO, Lopes DS, da Silva CV, da Silva MS, Borges BC, Silva MJB, Rodrigues RS, Rodrigues VM, Von Poelhsitz G, Yoneyama KAG Tags: J Inorg Biochem Source Type: research

Interactions of acetamide and acrylamide with heme models: Synthesis, infrared spectra, and solid state molecular structures of five- and six-coordinate ferric porphyrin derivatives.
Abstract The amide functional group is a fundamental building block of proteins, but is also present in several industrial chemicals such as acetamide and acrylamide. Some acetamide derivatives are known to deplete cytoplasmic heme, and some acrylamide derivatives are known to cause porphyria and may activate soluble guanylyl cyclase through a heme-dependent mechanism. We have prepared a representative set of six-coordinate acetamide and acrylamide (L) complexes of iron porphyrins of the form [(por)Fe(L)2]ClO4 (por = TPP (tetraphenylporphyrinato dianion), T(p-OMe)PP (tetrakis(p-methoxyphenyl)porphyrinato diani...
Source: Journal of Inorganic Biochemistry - March 4, 2019 Category: Biochemistry Authors: Xu N, Guan Y, Nguyen N, Lingafelt C, Powell DR, Richter-Addo GB Tags: J Inorg Biochem Source Type: research

Oligomerization of cytochrome c, myoglobin, and related heme proteins by 3D domain swapping.
Abstract Oligomerization of heme proteins is useful for construction of new materials with cooperative and systematic functions; thus, diverse methods have been applied for construction of artificial heme protein oligomers. Three-dimensional (3D) domain swapping is a protein oligomerization phenomenon that exchanges the same domain or secondary structural element between molecules. 3D domain swapping was first reported in 1994; since then many proteins have been reported to domain swap. Our research group has been showing that various heme proteins domain swap. We also found that domain swapping of heme proteins o...
Source: Journal of Inorganic Biochemistry - March 3, 2019 Category: Biochemistry Authors: Hirota S Tags: J Inorg Biochem Source Type: research

Synthesis, characterization and antibacterial activity of Zn(II) coordination polymer.
In this study, the zinc(II)-based coordination polymer, [Zn(CPDA)(NO3)2)](CPDA = 1,2-cyclopentanedicarboxylic acid) (1), had been successfully synthesized according to the hydrothermal method. Afterwards, 1 had been characterized by means of single crystal and power X-ray diffraction, elemental analysis, thermogravimetric analysis and infrared spectrum techniques. In addition, the antibacterial activities in vitro had been evaluated towards Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), respectively, through the growth inhibition and inhibition zone experimental methods. Our results indicated that 1 ...
Source: Journal of Inorganic Biochemistry - February 27, 2019 Category: Biochemistry Authors: Wang D, Xing X, Ye X, Chen Z, Gou Z, Wu D Tags: J Inorg Biochem Source Type: research

Palladium(II) complexes with salicylaldehyde ligands: Synthesis, characterization, structure, in vitro and in silico study of the interaction with calf-thymus DNA and albumins.
Abstract The synthesis and characterization of four palladium(II) complexes with substituted salicylaldehydes (X-saloH) having the general formula [Pd(X-salo)2] was undertaken. The complexes are formulated as [Pd(3-OCH3-salo)2] 1, [Pd(5-NO2-salo)2] 2, [Pd(5-Cl-salo)2] 3, and [Pd(5-Br-salo)2] 4. The structure of complex 1 was verified by single-crystal X-ray crystallography. Spectroscopic (UV-vis), and physicochemical (viscosity measurements) techniques were employed in order to study the binding of the complexes with calf-thymus (CT) DNA, while ethidium bromide (EB) displacement studies, performed by fluorescence ...
Source: Journal of Inorganic Biochemistry - February 24, 2019 Category: Biochemistry Authors: Zianna A, Geromichalos GD, Hatzidimitriou AG, Coutouli-Argyropoulou E, Lalia-Kantouri M, Psomas G Tags: J Inorg Biochem Source Type: research

Anticancer activity and mechanism of bis-pyrimidine based dimetallic Ru(II)( η6-p-cymene) complex in human non-small cell lung cancer via p53-dependent pathway.
Anticancer activity and mechanism of bis-pyrimidine based dimetallic Ru(II)(η6-p-cymene) complex in human non-small cell lung cancer via p53-dependent pathway. J Inorg Biochem. 2019 Feb 23;194:52-64 Authors: Bhatti MZ, Ali A, Duong HQ, Chen J, Rahman FU Abstract Non-small cell lung cancer (NSCLC) is the most common cancer worldwide, which is related with poor prognosis and resistance to chemotherapy. Notably, ruthenium-based complexes have emerged as good alternative to the currently used platinum-based drugs for cancer therapy. In the present study, we synthesized a novel bis-pyrimidine based lig...
Source: Journal of Inorganic Biochemistry - February 23, 2019 Category: Biochemistry Authors: Bhatti MZ, Ali A, Duong HQ, Chen J, Rahman FU Tags: J Inorg Biochem Source Type: research