Synthesis, characterization and albumin binding capabilities of quinizarin containing ternary cobalt(III) complexes.
cute;A, Buglyó P Abstract Four Co(III) ternary complexes with the composition of [(Co(4 N))2(quin)](ClO4)4 or [(Co(4 N))2(quinS)](ClO4)3, where 4 N = tris(2-aminoethyl)amine (tren) or tris(2-pyridylmethyl)amine (tpa), quinH2 = quinizarin (1,4-dihydroxy-9,10-anthraquinone), quinSH3 = quinizarin-2-sulfonic acid (1,4-dihydroxy-9,10-anthraquinone-2-sulfonic acid), were synthesized, characterized and their human serum albumin (HSA) binding capabilities were also tested. The complexes can be considered as likely chaperons of quinizarins which are structural models for ...
Source: Journal of Inorganic Biochemistry - December 16, 2019 Category: Biochemistry Authors: Kozsup M, Dömötör O, Nagy S, Farkas E, Enyedy ÉA, Buglyó P Tags: J Inorg Biochem Source Type: research

Urease inhibition studies of six Ni(II), Co(II) and Cu(II) complexes with two sexidentate N2O4-donor bis-Schiff base ligands: An experimental and DFT computational study.
Abstract Six novel complexes, [Ni(C36H34N2O10)]·2.25CH3OH·0.5C4H10O (1), [Co(C36H34N2O10)] (2), [Cu(C36H34N2O10)]·2CH3OH (3), [Ni(C36H32N2O8Cl2)]·2CH3OH (4), [Co(C36H32N2O8Cl2)]·4CH3OH (5) and [Cu(C36H32N2O8Cl2)]·2CH3OH (6) with two sexidentate N2O4-donor bis-Schiff base ligands (C36H34N2O10 = 1,2-bis(2-methoxy-6-formylphenoxy)ethane-l-tyrosine; C36H32N2O8Cl2 = 1,2-bis(2-methoxy-6-formylphenoxy)ethane-l-4-chlorophenylalanine) have been synthesized and structurally characterized. Theoretical calculation of the six complexes was carried out by density fun...
Source: Journal of Inorganic Biochemistry - December 15, 2019 Category: Biochemistry Authors: Wang H, Xu C, Zhang X, Zhang D, Jin F, Fan Y Tags: J Inorg Biochem Source Type: research

Aluminium alters mineral composition and polyphenol metabolism in leaves of tea plants (Camellia sinensis).
In conclusion, low pH and enhanced Al availability to tea plants have a strong influence on the polyphenolic pattern of tealeaves and therefore may alter both the leaves' antioxidant properties and their ability to bind Al and Fe in non-toxic form. PMID: 31862583 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - December 11, 2019 Category: Biochemistry Authors: Tolrà R, Martos S, Hajiboland R, Poschenrieder C Tags: J Inorg Biochem Source Type: research

Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles.
Abstract The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV-Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorptio...
Source: Journal of Inorganic Biochemistry - December 10, 2019 Category: Biochemistry Authors: Laban B, Ralević U, Petrović S, Leskovac A, Vasić-Anićijević D, Marković M, Vasić V Tags: J Inorg Biochem Source Type: research

Gallium-68 and scandium-44 labelled radiotracers based on curcumin structure linked to bifunctional chelators: Synthesis and characterization of potential PET radiotracers.
i M Abstract Curcumin metal complexes showed widespread applications in medicine and can be exploited as a lead structure for developing new tracers for nuclear medicine application. Herein, the synthesis, chemical characterization and radiolabelling with gallium-68 and scandium-44 of two new targeting vectors based on curcumin scaffolds and linked to the chelators 1,4,7-triazacyclononane,1-glutaric acid-4,7-acetic acid (NODAGA) and 1,4-bis(carboxymethyl)-6-[bis(carboxymethyl)]amino-6-methylperhydro-1,4-diazepine (AAZTA) are reported. Synthesis of the precursors could be achieved with a 13% and 11% yield and radio...
Source: Journal of Inorganic Biochemistry - December 4, 2019 Category: Biochemistry Authors: Orteca G, Sinnes JP, Rubagotti S, Iori M, Capponi PC, Piel M, Rösch F, Ferrari E, Asti M Tags: J Inorg Biochem Source Type: research

Structural zinc binding sites shaped for greater works: Structure-function relations in classical zinc finger, hook and clasp domains.
Abstract Metal ions are essential elements present in biological systems able to facilitate many cellular processes including proliferation, signaling, DNA synthesis and repair. Zinc ion (Zn(II)) is an important cofactor for numerous biochemical reactions. Commonly, structural zinc sites demonstrate high Zn(II) affinity and compact architecture required for sequence-specific macromolecule binding. However, how Zn(II)-dependent proteins fold, how their dissociation occurs, and which factors modulate zinc protein affinity as well as stability remains not fully understood. The molecular rules governing precise regula...
Source: Journal of Inorganic Biochemistry - December 4, 2019 Category: Biochemistry Authors: Padjasek M, Kocyła A, Kluska K, Kerber O, Tran JB, Krężel A Tags: J Inorg Biochem Source Type: research

Triplet of cysteines - Coordinational riddle?
In this study, the interaction of Zn2+, Cd2+ and Ni2+ ions with four peptides containing cysteine triplet motif were studied by potentiometric and spectroscopic methods. The main goal of this research was to answer the question how effectively three thiols, each being next to other, are able to bind single metal ion. Two of peptides contain additional, fourth cysteine residue, separated from triplet by two and three other amino acid residues. As results show, all three cysteine residues in the CCC motif are able to participate in the coordination of the metal ion (Cd2+, Zn2+). Except cysteine thiol groups, amide nitrogen a...
Source: Journal of Inorganic Biochemistry - December 4, 2019 Category: Biochemistry Authors: Krzywoszyńska K, Swiątek-Kozłowska J, Potocki S, Ostrowska M, Kozłowski H Tags: J Inorg Biochem Source Type: research

Ternary copper(II) complex of 5-hydroxytryptophan and 1,10-phenanthroline with several pharmacological properties and an adequate safety profile.
We report the synthesis and biological evaluation of a ternary copper complex, [Cu(5HTP)(phen)(H2O)](NO3).2H2O, with the antioxidant agent 5-hydroxytryptophan (5-HTP) and phenanthroline (phen, added to improve its lipophilicity and membrane transport). The crystal structure of the complex was determined by X-ray diffraction methods. The complex showed antioxidant, antimicrobial, antitumor and antimetastatic properties with an adequate safety profile. The interaction of the metal with phen promotes cellular copper accumulation and cytotoxicity on human lung A549 cell line (IC50 = 3.6 μM). Furthermore, the ...
Source: Journal of Inorganic Biochemistry - December 4, 2019 Category: Biochemistry Authors: Naso LG, Martínez Medina JJ, D'Alessandro F, Rey M, Rizzi A, Piro OE, Echeverría GA, Ferrer EG, Williams PAM Tags: J Inorg Biochem Source Type: research

DFT analysis, spectroscopic study and biological activity of a newly synthesized benzoylhydrazone binuclear Cu(II) complex.
Abstract The current work reports a joint experimental and theoretical study of a novel Cu(II) complex [CuL(μ-CH3COO)]2, based on 2-acetylpyridine-benzoylhydrazone ligand (HL). The two Cu(II) atoms are five-coordinated, consisting of three NNO-donor atoms from the hidrazone ligand connected by acetate bridges. In addition to the structural analysis, the complete characterization includes magnetic susceptibility, elemental analysis, FT-IR and UV-Vis. Two different approaches of Density Functional Theory (DFT) with localized basis set and plane waves were performed. The theoretical calculations were used to optim...
Source: Journal of Inorganic Biochemistry - November 28, 2019 Category: Biochemistry Authors: Santiago PHO, Tiago FS, Castro MS, Souza PEN, Martins JBL, Gatto CC Tags: J Inorg Biochem Source Type: research

Anti-leishmanial activity and cytotoxicity of a series of tris-aryl Sb(V) mandelate cyclometallate complexes.
Abstract A series of ten cyclometallates and two μ2-peroxo bridged tris-aryl Sb(V) complexes derived from R/S-mandelic acid (= R/S-ManH2) were synthesised and characterised. As confirmed by X-ray crystallography the complexes 1Sr/s, [Sb(o-tol)3(man)], 2Sr/s, [Sb(m-tol)3(man)], 4Sr/s, [Sb(o-PhOMe)3(man)], 5Sr/s, [Sb(Mes)3(man)] and 6Sr/s, [Sb(p-tert-BuPh)3(man)] are all cyclometallates. Complexes 3Sr/s, [(Sb(p-tol)3(manH)2O2], contain a bridging O22- anion in the solid-state but convert to the cyclometallates in DMSO solution with concomitant release of H2O2 and formation of complexes [Sb(p-tol)3(man)], 3Sr'/s'....
Source: Journal of Inorganic Biochemistry - November 22, 2019 Category: Biochemistry Authors: Duffin RN, Blair VL, Kedzierski L, Andrews PC Tags: J Inorg Biochem Source Type: research

Influence of synthesis methods on the internalization of fluorescent gold nanoparticles into glioblastoma stem-like cells.
D, Janiak C Abstract Glioblastoma (GBM) is an aggressive disease with currently no satisfying treatment option available. GBM cells with stem cell properties are thought to be responsible for the initiation and propagation of the disease, as well as main contributors to the emergence of therapy resistance. In this work, we developed a novel method to synthesize fluorescent gold nanoparticles as potential drug and gene delivery systems for GBM therapy, able to penetrate three-dimensional stem cell selected patient-derived GBM neurosphere systems in vitro. By using polyethylene imine (PEI) as a stabilizer and reduci...
Source: Journal of Inorganic Biochemistry - November 22, 2019 Category: Biochemistry Authors: Giesen B, Nickel AC, Garzón Manjón A, Vargas Toscano A, Scheu C, Kahlert UD, Janiak C Tags: J Inorg Biochem Source Type: research

Modified EDTA selectively recognized Cu2+ and its application in the disaggregation of β-amyloid-Cu (II)/Zn (II) aggregates.
Modified EDTA selectively recognized Cu2+ and its application in the disaggregation of β-amyloid-Cu (II)/Zn (II) aggregates. J Inorg Biochem. 2019 Nov 23;203:110929 Authors: Chang R, Chen X, Yu H, Tan G, Wen H, Huang J, Hao Z Abstract The accumulation of the β-amyloid (Aβ) aggregates induced by Cu2+/Zn2+ in conjunction with toxicity is closely related to Alzheimer's disease (AD). Herein, we intended to improve the efficiency and selectivity of traditional chelator ethylenediaminetetraacetic acid (EDTA) combined with a fluorescent group 4-aminosalicylic acid (4-ASA)to acquire a novel pot...
Source: Journal of Inorganic Biochemistry - November 22, 2019 Category: Biochemistry Authors: Chang R, Chen X, Yu H, Tan G, Wen H, Huang J, Hao Z Tags: J Inorg Biochem Source Type: research

Comparative study of the antitumoral activity of phosphine-thiosemicarbazone gold(I) complexes obtained by different methodologies.
aacute;lez-Noya AM, Pedrido R Abstract A series of phosphino-thiosemicarbazone gold(I) dinuclear complexes obtained by two different synthetic procedures have been prepared. All the compounds have been spectroscopically characterized including single crystal X ray diffraction analysis in some of cases. [Au2(HL1)Cl2] (1), [Au2(HL2)2]Cl2 (2) and [Au2(HL3)2]Cl2 (3) have been prepared by chemical synthesis using a gold(III) salt as precursor; while [Au2(L1)2] (4), [Au2(L2)2]∙2CH3CN (5) and [Au2(L3)2] (6) have been isolated from an electrochemical synthesis (HLn = 2-[2-(diphenylphosphanyl)-benzylidene]-N-R-thiose...
Source: Journal of Inorganic Biochemistry - November 21, 2019 Category: Biochemistry Authors: González-Barcia LM, Fernández-Fariña S, Rodríguez-Silva L, Bermejo MR, González-Noya AM, Pedrido R Tags: J Inorg Biochem Source Type: research

Coordination chemistry and catalytic applications of Pd(II)-, and Ni(II)-sulfosalan complexes in aqueous media.
te; F Abstract With the aim of identifying new types of water-soluble catalyst precursors for modification of biological membranes by homogeneous hydrogenation in aqueous solution and under mild conditions, we have performed detailed equilibrium and spectroscopic characterization of complex formation between nickel(II) or palladium(II) and salan-type ligands sulfonated in their aromatic rings (N,N'-bis(2-hydroxy-5-sulfonatobenzyl)-1,4-diaminoethane (HSS), N,N'-bis(2-hydroxy-5-sulfonatobenzyl)-1,4-diaminopropane (PrHSS) and N,N'-bis(2-hydroxy-5-sulfonatobenzyl)-1,4-diaminobutane (BuHSS)) in the slightly acidic-alka...
Source: Journal of Inorganic Biochemistry - November 21, 2019 Category: Biochemistry Authors: Lihi N, Bunda S, Udvardy A, Joó F Tags: J Inorg Biochem Source Type: research

Cognition and behavior in sheep repetitively inoculated with aluminum adjuvant-containing vaccines or aluminum adjuvant only.
This study is the first to describe behavioral changes in sheep after having received repetitive injections of Al-containing products, and may explain some of the clinical signs observed in ovine ASIA syndrome. PMID: 31783216 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - November 19, 2019 Category: Biochemistry Authors: Asín J, Pascual-Alonso M, Pinczowski P, Gimeno M, Pérez M, Muniesa A, de Pablo-Maiso L, de Blas I, Lacasta D, Fernández A, de Andrés D, Reina R, Luján L Tags: J Inorg Biochem Source Type: research

Importance of the iron-sulfur component and of the siroheme modification in the resting state of sulfite reductase.
cu R Abstract The active site of sulfite reductase (SiR) consists of an unusual siroheme-Fe4S4 assembly coupled via a cysteinate sulfur, and serves for multi-electron reduction reactions. Clear explanations have not been demonstrated for the reasons behind the choice of siroheme (vs. other types of heme) or for the single-atom coupling to an Fe4S4 center (as opposed to simple adjacency or to coupling via chains consisting of more than one atom). Possible explanations for these choices have previously been invoked, relating to the control of the spin state of the substrate-binding (siro)heme iron, modulation of the...
Source: Journal of Inorganic Biochemistry - November 17, 2019 Category: Biochemistry Authors: Brânzanic AMV, Ryde U, Silaghi-Dumitrescu R Tags: J Inorg Biochem Source Type: research

Copper(I) complexes with phosphines P(p-OCH3-Ph)2CH2OH and P(p-OCH3-Ph)2CH2SarGly. Synthesis, multimodal DNA interactions, and prooxidative and in vitro antiproliferative activity.
oł A Abstract Phosphonium salt (p-OCH3-Ph)2P(CH2OH)2Cl (MPOHC), derived phosphine ligands without and with SarGly (Sarcosine-Glycine) peptide carrier P(p-OCH3-Ph)2CH2OH (MPOH) and P(p-OCH3-Ph)2CH2SarGly (MPSG), respectively, and two copper(I) complexes [Cu(I)(dmp)(MPOH)] (1-MPOH; dmp = (2,9-dimethyl-1,10-phenanthroline)) and [Cu(I)(dmp)(MPSG)] (1-MPSG) were synthesized. The resulting compounds were characterized by elemental analysis, 1D and 2D NMR and UV-Vis absorption spectroscopies, mass spectrometry, cyclic voltammetry and by X-ray diffraction analysis. Cytotoxicity of all compounds was evaluated in...
Source: Journal of Inorganic Biochemistry - November 14, 2019 Category: Biochemistry Authors: Komarnicka UK, Kozieł S, Zabierowski P, Kruszyński R, Lesiów MK, Tisato F, Porchia M, Kyzioł A Tags: J Inorg Biochem Source Type: research

Salen ‑manganese complexes for controlling ROS damage: Neuroprotective effects, antioxidant activity and kinetic studies.
Salen‑manganese complexes for controlling ROS damage: Neuroprotective effects, antioxidant activity and kinetic studies. J Inorg Biochem. 2019 Nov 14;203:110918 Authors: Rouco L, Liberato A, Fernández-Trujillo MJ, Máñez A, Basallote MG, Alvariño R, Alfonso A, Botana LM, Maneiro M Abstract A new manganese(III) complex [MnL1(DCA)(H2O)](H2O),1 [H2L1 is the chelating ligand N,N'-bis(2-hydroxy-3-methoxybenzylidene)-1,2-diaminopropane, and DCA is dicyanamide], has been prepared and characterized by different analytical and spectroscopic techniques. The tetragonally elongated oc...
Source: Journal of Inorganic Biochemistry - November 13, 2019 Category: Biochemistry Authors: Rouco L, Liberato A, Fernández-Trujillo MJ, Máñez A, Basallote MG, Alvariño R, Alfonso A, Botana LM, Maneiro M Tags: J Inorg Biochem Source Type: research

Investigation of the requirements for efficient and selective cytochrome P450 monooxygenase catalysis across different reactions.
Abstract The cytochrome P450 metalloenzyme (CYP) CYP199A4 from Rhodopseudomonas palustris HaA2 catalyzes the highly efficient oxidation of para-substituted benzoic acids. Here we determined crystal structures of CYP199A4, and the binding and turnover parameters, with different meta-substituted benzoic acids in order to establish which criteria are important for efficient catalysis. When compared to the para isomers, the meta-substituted benzoic acids were less efficiently oxidized. For example, 3-formylbenzoic acid was oxidized with lower activity than the equivalent para isomer and 3-methoxybenzoic acid did not u...
Source: Journal of Inorganic Biochemistry - November 12, 2019 Category: Biochemistry Authors: Podgorski MN, Coleman T, Chao RR, De Voss JJ, Bruning JB, Bell SG Tags: J Inorg Biochem Source Type: research

The interaction of aluminium-based adjuvants with THP-1 macrophages in vitro: Implications for cellular survival and systemic translocation.
Abstract Within clinical vaccinations, recombinant antigens are routinely entrapped inside or adsorbed onto the surface of aluminium salts in order to increase their immunological potency in vivo. The efficacy of these immunisations is highly dependent upon the recognition and uptake of these complexes by professional phagocytes and their subsequent delivery to the draining lymph nodes for further immunological processing. While monocytes have been shown to internalise aluminium adjuvants and their adsorbates, the role of macrophages in this respect has not been fully established. Furthermore, this study explored ...
Source: Journal of Inorganic Biochemistry - November 11, 2019 Category: Biochemistry Authors: Shardlow E, Mold M, Exley C Tags: J Inorg Biochem Source Type: research

Molecular docking of polyoxometalates as potential α-glucosidase inhibitors.
Molecular docking of polyoxometalates as potential α-glucosidase inhibitors. J Inorg Biochem. 2019 Nov 12;203:110914 Authors: Hu J, Wang L, Wang F, Chi G, Liu G, Sun L Abstract α-Glucosidase is an important target enzyme for the treatment of type 2 diabetes in humans. In our previous studies, it was found that polyoxometalates exhibited an effective inhibitory effect on the activity of α-glucosidase, while polyoxometalates have the characteristics of structural diversity and unique properties. Herein, we investigated the inhibition of two different series of polyoxometalates on &alph...
Source: Journal of Inorganic Biochemistry - November 11, 2019 Category: Biochemistry Authors: Hu J, Wang L, Wang F, Chi G, Liu G, Sun L Tags: J Inorg Biochem Source Type: research

The inhibition of lactoperoxidase catalytic activity through mesna (2-mercaptoethane sodium sulfonate).
Abstract Here, we show that mesna (sodium-2-mercaptoethane sulfonate), primarily used to prevent nephrotoxicity and urinary tract toxicity caused by chemotherapeutic agents such as cyclophosphamide and ifosfamide, modulates the catalytic activity of lactoperoxidase (LPO) by binding tightly to the enzyme, functioning either as a one electron substrate for LPO Compounds I and II, destabilizing Compound III. Lactoperoxidase is a hemoprotein that utilizes hydrogen peroxide (H2O2) and thiocyanate (SCN-) to produce hypothiocyanous acid (HOSCN), an antimicrobial agent also thought to be associated with carcinogenesis. Ou...
Source: Journal of Inorganic Biochemistry - November 11, 2019 Category: Biochemistry Authors: Jahanbakhsh S, Dekhne MS, Kohan-Ghadr HR, Bai D, Awonuga A, Morris RT, Yang Z, Abu-Soud HM Tags: J Inorg Biochem Source Type: research

Exploring DNA binding ability of two novel α-N-heterocyclic thiosemicarbazone palladium(II) complexes.
Exploring DNA binding ability of two novel α-N-heterocyclic thiosemicarbazone palladium(II) complexes. J Inorg Biochem. 2019 Nov 06;203:110875 Authors: Fuentes L, Quiroga AG, Organero JA, Matesanz AI Abstract One mononuclear and another dinuclear Pd(II) complexes bearing a α-N-heterocyclic thiosemicarbazone ligand have been synthesized, fully characterized and studied as biological agents. In both complexes, the palladium center is coordinated to 3,5-diacetyl-1,2,4-triazol bis(N4,N4-dimethylthiosemicarbazone) via three sites (N, N and S). Their binding ability to DNA has been evaluated usi...
Source: Journal of Inorganic Biochemistry - November 5, 2019 Category: Biochemistry Authors: Fuentes L, Quiroga AG, Organero JA, Matesanz AI Tags: J Inorg Biochem Source Type: research

The mechanism for oxygen reduction in the C family cbb3 cytochrome c oxidases - Implications for the proton pumping stoichiometry.
Abstract Cytochrome c oxidases (CcOs) couple the exergonic reduction of molecular oxygen to proton pumping across the membrane in which they are embedded, thereby conserving a significant part of the free energy. The A family CcOs are known to pump four protons per oxygen molecule, while there is no consensus regarding the proton pumping stoichiometry for the C family cbb3 oxidases. Hybrid density functional theory is used here to investigate the catalytic mechanism for oxygen reduction in cbb3 oxidases. A surprising result is that the barrier for O O bond cleavage at the mixed valence reduction level seems to be ...
Source: Journal of Inorganic Biochemistry - October 30, 2019 Category: Biochemistry Authors: Blomberg MRA Tags: J Inorg Biochem Source Type: research

Polynuclear zinc(II) complexes of thiosemicarbazone: Synthesis, X-ray structure and biological evaluation.
Abstract Two new dimeric Zn(II) ([{ZnL1(DMSO2)}2]·DMSO (1), [{ZnL2Cl}2] (2)) and a novel tetrameric Zn(II) complex ([(Zn2L3)2(μ-OAc)2(μ3-O)2] (3)), where H2L1 = 4-(p-methoxyphenyl) thiosemicarbazone of o-hydroxynapthaldehyde, HL2 = 4-(p-methoxyphenyl)thiosemicarbazone of benzoyl pyridine and H2L3 = 4-(p-chlorophenyl)thiosemicarbazone of o-vanillin are reported. Ligands and their complexes were characterized by spectroscopic and single crystal X-ray diffraction techniques. In addition, the complexes exhibited good binding affinity towards HSA (1012 M-1), which is supported by their abilit...
Source: Journal of Inorganic Biochemistry - October 30, 2019 Category: Biochemistry Authors: Saswati, Mohanty M, Banerjee A, Biswal S, Horn A, Schenk G, Brzezinski K, Sinn E, Reuter H, Dinda R Tags: J Inorg Biochem Source Type: research

An Erlotinib gold(I) conjugate for combating triple-negative breast cancer.
uiz J Abstract An Erlotinib triphenylphosphane gold(I) conjugate has been prepared from AuCl(PPh3) and its crystal structure has been established by X-ray diffraction, showing a metallo-helicate formation. IC50 values of the new gold conjugate were calculated towards a panel of human tumor cell lines representative of breast (MCF-7, MDA-MB-231) and colon (HT-29) cancer cells. Overall, the gold conjugate exhibited higher cytotoxic activity than that of Erlotinib against the cancer cells studied. Particularly, the antiproliferative effect of the conjugate demonstrated to be 68-fold higher than Erlotinib in highly me...
Source: Journal of Inorganic Biochemistry - October 29, 2019 Category: Biochemistry Authors: Ortega E, Zamora A, Basu U, Lippmann P, Rodríguez V, Janiak C, Ott I, Ruiz J Tags: J Inorg Biochem Source Type: research

Refinement of protein Fe(II) binding characteristics utilizing a competition assay exploiting small molecule ferrous chelators.
Abstract Iron is the most prevalent metal in biology. Its chemical and redox versatility allows it to direct activity of many Fe binding proteins. While iron's biological applications are diverse, challenges inherent in having Fe(II) present at high abundance means cells must ensure delivery to the correct recipient, while also ensuring its chemistry is regulated. Having a detailed understanding of the biophysical characteristics of a protein's iron binding characteristics allows us to understand general cellular metal homeostasis events. Unfortunately, most spectroscopic techniques available to measure metal bind...
Source: Journal of Inorganic Biochemistry - October 28, 2019 Category: Biochemistry Authors: Koebke KJ, Batelu S, Kandegedara A, Smith SR, Stemmler TL Tags: J Inorg Biochem Source Type: research

Heterobinuclear copper(II) ‑platinum(II) complexes with oxindolimine ligands: Interactions with DNA, and inhibition of kinase and alkaline phosphatase proteins.
Heterobinuclear copper(II)‑platinum(II) complexes with oxindolimine ligands: Interactions with DNA, and inhibition of kinase and alkaline phosphatase proteins. J Inorg Biochem. 2019 Oct 28;203:110863 Authors: Aranda EE, da Luz JS, Oliveira CC, Divina Petersen PA, Petrilli HM, da Costa Ferreira AM Abstract Two mononuclear copper(II) compounds, [Cu(isad)(H2O)Cl]Cl 1 and [Cu(isah)(H2O)Cl]Cl 2, and its corresponding heterobinuclear species containing also platinum(II), [CuCl(isad)Pt(NH3)Cl2] 3 and [CuCl(isah)Pt(NH3)Cl2] 4 (where isad and isah are oxindolimine ligands, (E)-3-(2-(3-aminopropylamino)ethyli...
Source: Journal of Inorganic Biochemistry - October 27, 2019 Category: Biochemistry Authors: Aranda EE, da Luz JS, Oliveira CC, Divina Petersen PA, Petrilli HM, da Costa Ferreira AM Tags: J Inorg Biochem Source Type: research

Synthesis, reactivity, X-ray characterization and docking studies of N7/N9-(2-pyrimidyl)-adenine derivatives.
M, Ortega-Castro J, Molins E, Frontera A Abstract The reaction of adenine with 2-chloropyrimidine yields as a major product the unexpected N7-(2-pyrimidyl)-adenine (1) and as a minor one N9-(2-pyrimidyl)-adenine (2). Both compounds have been characterized by X-ray diffraction analysis. Moreover, we report the formation of a 1:1 co-crystal (3) composed by compound (1) and adenine that was formed serendipitously during the synthesis of (1). Unexpectedly, the treatment of (1) with Brönsted acids like HCl or HNO3 causes the opening of the imidazole ring of the N7-substituted adenine, yielding N5-(pyrimidin-2-yl)...
Source: Journal of Inorganic Biochemistry - October 21, 2019 Category: Biochemistry Authors: Martínez D, Pérez A, Cañellas S, Silió I, Lancho A, García-Raso A, Fiol JJ, Terrón A, Barceló-Oliver M, Ortega-Castro J, Molins E, Frontera A Tags: J Inorg Biochem Source Type: research

Structural modification of nanohydroxyapatite Ca10(PO4)6(OH)2 related to Eu3+ and Sr2+ ions doping and its spectroscopic and antimicrobial properties.
Abstract The Eu3+ and Sr2+ ions co-doped hydroxyapatite nanopowders (Ca10(PO4)6(OH)2) were synthesized via a precipitation method and post heat-treated at 500 °C. The concentration of Eu3+ ions was established in the range of 0.5-5 mol% to investigate the site occupancy preference. The concentration of Sr2+ ions was set at 5 mol%. The structural and morphological properties of the obtained materials were studied by an X-ray powder diffraction, a transmission electron microscopy techniques and infrared spectroscopy. As synthesized nanoparticles were in the range of 11-17 nm and annealed particles were i...
Source: Journal of Inorganic Biochemistry - October 21, 2019 Category: Biochemistry Authors: Szyszka K, Rewak-Soroczynska J, Dorotkiewicz-Jach A, Ledwa KA, Piecuch A, Giersig M, Drulis-Kawa Z, Wiglusz RJ Tags: J Inorg Biochem Source Type: research

Fabrication of biodegradable Mn-doped mesoporous silica nanoparticles for pH/redox dual response drug delivery.
Abstract Mn-doped mesoporous silica (MMS) nanocarriers were prepared by in situ doping Mn component for drug delivery. In the acidic or reductive microenvironment of tumor, the MnO bond can be broken up, and Mn components would be released from the silica framework, thus accelerating the degradation of the silica nanocarrier. Due to the mesoporous structure, MMS nanocarriers have a high specific surface area of 1125.56 m2/g, which is suitable for loading and controlled release of drug. And the encapsulation ratio of Dox and drug loading of MMS and MMS/PDA is 98.08%, 39.54% and 38.81% respectively. After loading ...
Source: Journal of Inorganic Biochemistry - October 21, 2019 Category: Biochemistry Authors: Li X, Zhang X, Zhao Y, Sun L Tags: J Inorg Biochem Source Type: research

On the path to gold: Monoanionic Au bisdithiolate complexes with antimicrobial and antitumor activities.
Marques F Abstract The emergence of resistance to antimicrobial and anticancer drugs poses severe threats to public health worldwide, highlighting the need for more efficient treatments. Here, four monoanionic Au bisdithiolate complexes [Au(mnt)2]- (where mnt = 1,1-dicyanoethylene-2,2-dithiolate)(1), [Au(i-mnt)2]- (where i-mnt = 2,2-dicyanoethylene-1,1-dithiolate)(2), [Au(cdc)2]- (where cdc = cyanodithioimido carbonate)(3), and [Au(qdt)2]- (where qdt = quinoxaline-2,3-dithiolate)(4) were screened for their antimicrobial and antitumor activities. Complexes 3 and 4 showed antibacterial activity agai...
Source: Journal of Inorganic Biochemistry - October 21, 2019 Category: Biochemistry Authors: Sousa SA, Leitão JH, Silva RAL, Belo D, Santos IC, Guerreiro JF, Martins M, Fontinha D, Prudêncio M, Almeida M, Lorcy D, Marques F Tags: J Inorg Biochem Source Type: research

Release of trivalent chromium from serum transferrin is sufficiently rapid to be physiologically relevant.
Abstract Transferrin, the major iron transport protein in the blood, apparently also transports trivalent chromium in vivo via endocytosis. Recent in vitro studies have, however, suggested that the release of chromic ions from serum transferrin is too slow to be biologically relevant. Consequently, the release of chromium(III) from human serum transferrin has been examined under conditions mimicking an endosome during endocytosis. At pH 4.5 and 5.5, the release of Cr(III) from transferrin occurs rapidly from the weak binding site. While appreciably slower, the release of Cr(III) from the tighter site in the pres...
Source: Journal of Inorganic Biochemistry - October 19, 2019 Category: Biochemistry Authors: Edwards KC, Kim H, Vincent JB Tags: J Inorg Biochem Source Type: research

Antitumor activity of organoruthenium complexes with chelate aromatic ligands, derived from 1,10-phenantroline: Synthesis and biological activity.
Abstract The monocationic chloro complexes containing chelating N∩N ligands: [(η6-p-cymene)Ru(L1-4)Cl]+ (1-4), where L1 = 4-methyl-1,10-phenantroline, L2 = dipyrido[3,2-a:2',3'-c]phenazine, L3 = 11-chloro-dipyrido[3,2-a:2',3'-c]phenazine, L4 = 11-nitro-dipyrido[3,2-a:2',3'-c]phenazine; p-cymene = 1-methyl-4-isopropylbenzene) have been prepared and characterized as the hexafluorophosphate salts. The biological activity of 1-4 has been investigated in selected 2D monolayer cell cultures (A549, PANC-1, MDA-MB-231, MRC-5). All investigated ruthenium complexes showed similar or even better c...
Source: Journal of Inorganic Biochemistry - October 19, 2019 Category: Biochemistry Authors: Savić A, Gligorijević N, Aranđelović S, Dojčinović B, Kaczmarek AM, Radulović S, Van Deun R, Van Hecke K Tags: J Inorg Biochem Source Type: research

Adenine nucleobase directed supramolecular architectures based on ferrimagnetic heptanuclear copper(II) entities and benzenecarboxylate anions.
án P, Velasco LF Abstract Two planar organic anions, benzoate and benzene-1,4-dicarboxylate (terephthalate), have been selected as potential π-stacking intercalators among ferrimagnetic [Cu7(μ-adeninato)6(μ3-OH)6(μ-H2O)6]2+ heptameric discrete entities. The resulting supramolecular architecture is highly dependent on the negative charge density distribution, mainly located in the carboxylate groups of the organic anions. In this sense, the benzoate anion, with just one carboxylate group, does not allow its intercalation between the adeninato ligands as it would imply a high steric hindrance amon...
Source: Journal of Inorganic Biochemistry - October 4, 2019 Category: Biochemistry Authors: Pascual-Colino J, Beobide G, Castillo O, Lodewyckx P, Luque A, Pérez-Yáñez S, Román P, Velasco LF Tags: J Inorg Biochem Source Type: research

Synthesis, characterization, DNA/BSA interactions and in vitro cytotoxicity study of palladium(II) complexes of hispolon derivatives.
Abstract Thirteen novel palladium(II) complexes of the general formula [Pd(bipy)(O,O'-dkt)](PF6), (where bipy is 2,2'-bipyridine and O,O'-dkt is β-diketonate ligand hispolon or its derivative) have been prepared through a metal-ligand coordination method that involves spontaneous formation of the corresponding diketonate scaffold. The obtained palladium(II) complexes have been characterized by NMR spectroscopy, ESI-mass spectrometry as well as elemental analysis. The cytotoxicity analysis indicates that most of the obtained palladium(II) complexes show promising growth inhibition in three human cancer cell li...
Source: Journal of Inorganic Biochemistry - October 4, 2019 Category: Biochemistry Authors: Wei X, Yang Y, Ge J, Lin X, Liu D, Wang S, Zhang J, Zhou G, Li S Tags: J Inorg Biochem Source Type: research

Complexes containing benzimidazolyl-phenol ligands and Ln(III) ions: Synthesis, spectroscopic studies and preliminary cytotoxicity evaluation.
ranados O, Colorado-Peralta R Abstract Fourteen new complexes were obtained from Ln(III)(NO3)3∙n-H2O and the chromophores 2-(1H-benzo[d]imidazol-2-yl)-phenol (Bzp1) or 2-(5-methyl-1H-benzo[d]imidazol-2-yl)-phenol (Bzp2). The complete characterization allowed us to assign unequivocally the structures of all the complexes. The techniques used for this purpose were Ultraviolet-Visible (UV-Vis) and Fourier-Transform Infrared (FT-IR) spectroscopies, High-Resolution Mass Spectrometry (HRMS), Magnetic Susceptibility (MS), Elemental Analysis (EA) and Molar Conductivity (MC). HRMS allowed us to find the molecular ion and...
Source: Journal of Inorganic Biochemistry - September 10, 2019 Category: Biochemistry Authors: Hernández-Morales A, Rivera JM, López-Monteon A, Lagunes-Castro S, Castillo-Blum S, Cureño-Hernández K, Flores-Parra A, Villaseñor-Granados O, Colorado-Peralta R Tags: J Inorg Biochem Source Type: research

Correlation between the T1 copper reduction potential and catalytic activity of a small laccase.
Abstract Laccases are multicopper enzymes that catalyze oxidation of electron-rich substrates coupled to reduction of molecular oxygen to water. Since the Type 1 copper (T1 Cu) is the site where electrons are withdrawn from the substrate, it is assumed that the reduction potential of this copper correlates with enzyme activity. Herein, we studied the correlation of the T1 Cu reduction potential and the enzymatic activity of the small two-domain laccase Ssl1 from Streptomyces sviceus. For a systematic approach, we aimed to minimize any effects other than the reduction potential difference. To this end, we construct...
Source: Journal of Inorganic Biochemistry - September 8, 2019 Category: Biochemistry Authors: Olbrich AC, Schild JN, Urlacher VB Tags: J Inorg Biochem Source Type: research

MRI and fluorescence studies of Saccharomyces cerevisiae loaded with a bimodal Fe(III) T1 contrast agent.
Abstract Labeling of cells with paramagnetic metal complexes produces changes in MRI properties that have applications in cell tracking and identification. Here we show that fungi, specifically the budding yeast Saccharomyces cerevisiae, can be loaded with Fe(III) T1 contrast agents. Two Fe(III) macrocyclic complexes based on 1,4,7-triazacyclononane, with two pendant alcohol groups are prepared and studied as T1 relaxation MRI probes. To better visualize uptake and localization in the yeast cells, Fe(III) complexes have a fluorescent tag, consisting of either carbostyril or fluoromethyl coumarin. The Fe(III) compl...
Source: Journal of Inorganic Biochemistry - September 5, 2019 Category: Biochemistry Authors: Patel A, Asik D, Spernyak JA, Cullen PJ, Morrow JR Tags: J Inorg Biochem Source Type: research

Synthesis and anticancer evaluation of benzo-N-heterocycles transition metal complexes against esophageal cancer cell lines.
Abstract Three novel transition metal complexes, Cu(p-2-bmq)Cl2 (1), Zn(p-2-bmq)Cl2 (2) and [Co(p-2-bmq)Cl2]2 (3) (where p-2-bmq = 2-((1-(pyridin-2-yl)-1H-benzoimidazol-2-yl)methyl) quinolone, have been synthesized. The complexes were detected for their cytotoxicity in vitro against four human esophageal cancer cell lines (SMMC7721, BGC823, HCT116 and HT29) by MTT assay. The results showed that they all have anti-tumor cell proliferation activity. E specially, complex 1 exhibited significant cytotoxicity with IC50 value of 15.89 μM against SMMC7721 cells for 72 h. The morphological changes of nuclei by ...
Source: Journal of Inorganic Biochemistry - September 5, 2019 Category: Biochemistry Authors: Zhi S, Li Y, Qiang J, Hu J, Song W, Zhao J Tags: J Inorg Biochem Source Type: research

Kinetic analysis of a globin-coupled diguanylate cyclase, YddV: Effects of heme iron redox state, axial ligands, and heme distal mutations on catalysis.
ova M Abstract Heme-based oxygen sensors allow bacteria to regulate their activity based on local oxygen levels. YddV, a globin-coupled oxygen sensor with diguanylate cyclase activity from Escherichia coli, regulates cyclic-di-GMP synthesis based on oxygen availability. Stable and active samples of the full-length YddV protein were prepared by attaching it to maltose binding protein (MBP). To better understand the full-length protein's structure, the interactions between its domains were examined by performing a kinetic analysis. The diguanylate cyclase reaction catalyzed by YddV-MBP exhibited Michaelis-Menten kin...
Source: Journal of Inorganic Biochemistry - September 5, 2019 Category: Biochemistry Authors: Lengalova A, Fojtikova-Proskova V, Vavra J, Martínek V, Stranava M, Shimizu T, Martinkova M Tags: J Inorg Biochem Source Type: research

BioMOF@cellulose fabric composites for bioactive molecule delivery.
Abstract This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy. In situ synthesis of BioMOF on the cotton fabric was successfully achieved and the incorporation of NO and 5FU was studied. We have observed that BioMOF doped with FeII metal ions has a beneficial impact on NO incorporation. The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Atten...
Source: Journal of Inorganic Biochemistry - September 4, 2019 Category: Biochemistry Authors: Noorian SA, Hemmatinejad N, Navarro JAR Tags: J Inorg Biochem Source Type: research

Artificial bioinorganic clusters of dinuclear 3d-transition metal ions coordinated by an inorganic coordination ligand.
Abstract A new biologically relevant cubane-type artificial inorganic ligand was developed to support 3d-transition metal ions of Mn(II), Co(II), Ni(II), Zn(II), Fe(III), and also In(III). All of the reactions produce dinuclear cationic clusters of [({Co(tacn)}2HMo3O13∙H2O){M(H2O)2}]2n+ (M = Mn2+ (4Mn), Co2+ (4Co), Ni2+ (4Ni), Zn2+ (4Zn), n = 2; M = Fe3+ (5), In3+ (6), n = 4), where tacn is the abbreviation of 1,4,7-triazacyclononane ligand. Clusters 4 do not dissolve in any solvents at all to prevent the recrystallization or the further characterization whereas the high solubility of clusters 5 ...
Source: Journal of Inorganic Biochemistry - September 4, 2019 Category: Biochemistry Authors: Sugiarto, Kawamoto K, Hayashi Y Tags: J Inorg Biochem Source Type: research

Replacement of the heme axial lysine as a test of conformational adaptability in the truncated hemoglobin THB1.
Abstract Amino acid replacement is a useful strategy to assess the roles of axial heme ligands in the function of native heme proteins. THB1, the protein product of the Chlamydomonas reinhardtii THB1 gene, is a group 1 truncated hemoglobin that uses a lysine residue in the E helix (Lys53, at position E10 by reference to myoglobin) as an iron ligand at neutral pH. Phylogenetic evidence shows that many homologous proteins have a histidine, methionine or arginine at the same position. In THB1, these amino acids would each be expected to convey distinct reactive properties if replacing the native lysine as an axial li...
Source: Journal of Inorganic Biochemistry - September 3, 2019 Category: Biochemistry Authors: Nye DB, Johnson EA, Mai MH, Lecomte JTJ Tags: J Inorg Biochem Source Type: research

Structural insights for vanadium catecholates and iron ‑sulfur clusters obtained from multiple data analysis methods applied to electron spin relaxation data.
Structural insights for vanadium catecholates and iron‑sulfur clusters obtained from multiple data analysis methods applied to electron spin relaxation data. J Inorg Biochem. 2019 Sep 02;201:110806 Authors: Ngendahimana T, Ayikpoe R, Latham JA, Eaton GR, Eaton SS Abstract Electron paramagnetic resonance (EPR) inversion recovery curves for vanadium catecholates and iron‑sulfur clusters were analyzed with three models: the sum of two exponentials, a stretched exponential, and a model-free distribution of exponentials (UPEN). For all data sets studied fits with a stretched exponential were statistica...
Source: Journal of Inorganic Biochemistry - September 1, 2019 Category: Biochemistry Authors: Ngendahimana T, Ayikpoe R, Latham JA, Eaton GR, Eaton SS Tags: J Inorg Biochem Source Type: research

Platinum(II) coordination compounds with 4'-pyridyl functionalized 2,2':6',2 ″-terpyridines as an alternative to enhanced chemotherapy efficacy and reduced side-effects.
Platinum(II) coordination compounds with 4'-pyridyl functionalized 2,2':6',2″-terpyridines as an alternative to enhanced chemotherapy efficacy and reduced side-effects. J Inorg Biochem. 2019 Aug 30;201:110809 Authors: Altmann S, Choroba K, Skonieczna M, Zygadło D, Raczyńska-Szajgin M, Maroń A, Małecki JG, Szłapa-Kula A, Tomczyk M, Ratuszna A, Machura B, Szurko A Abstract Two platinum(II) coordination compounds, [PtCl(4'-R1-terpy)](SO3CF3) (1) and [PtCl(4'-R2-terpy)](SO3CF3) (2), with 4'-(2-pyridyl)-2,2':6',2″-terpyridine (4'-R1-terpy) or 4'-(3-pyridyl)-2,2':6',2″-terpyridine (4'...
Source: Journal of Inorganic Biochemistry - August 29, 2019 Category: Biochemistry Authors: Altmann S, Choroba K, Skonieczna M, Zygadło D, Raczyńska-Szajgin M, Maroń A, Małecki JG, Szłapa-Kula A, Tomczyk M, Ratuszna A, Machura B, Szurko A Tags: J Inorg Biochem Source Type: research

In vitro tyrosinase, acetylcholinesterase, and HSA evaluation of dioxidovanadium (V) complexes: An experimental and theoretical approach.
Abstract The present study reports the biological evaluation of vanadium(V) complexes (1-3) against three different proteins: tyrosinase, acetylcholinesterase (AChE), and human serum albumin (HSA), which were studied by spectroscopic techniques and molecular docking. Despite the synthesis and characterization of complexes 1 and 2 having already previously described, complex 3 is a novel dioxidovanadium(V) derivative. Complex 1 can activate both tyrosinase and AChE enzymes in about 11.5 and 47.0%, respectively. On the other hand, complexes 2 and 3 inhibited the same enzymes (1.30 and 46.0% for tyrosinase and 20.0 a...
Source: Journal of Inorganic Biochemistry - August 28, 2019 Category: Biochemistry Authors: Chaves OA, de Oliveira MCC, de Salles CMC, Martins FM, Iglesias BA, Back DF Tags: J Inorg Biochem Source Type: research

High antiparasitic activity of silver complexes of 5,7-dimethyl-1,2,4-triazolo[1,5 a]pyrimidine.
s JM, Sánchez-Moreno M Abstract Two dinuclear silver complexes containing 5,7-dimethyl[1,2,4]triazolo[1,5-a]pyrimidine (dmtp) were synthesized, [Ag2(dmtp)3]2[Ag2(dmtp)2](BF4)6(H2O)2 and [Ag2(dmtp)2(ClO4)2][Ag2(dmtp)2(H2O)2](ClO4)2. They have been spectroscopically and thermally characterized and their structures have been solved by single crystal X-ray diffraction. The compounds display fluorescence in the visible range (486 nm) when irradiated with UV light (265 nm). Both compounds, together with a previously reported analogue containing nitrate as counteranion, were assayed in vitro against three diff...
Source: Journal of Inorganic Biochemistry - August 28, 2019 Category: Biochemistry Authors: Esteban-Parra GM, Méndez-Arriaga JM, Rodríguez-Diéguez A, Quirós M, Salas JM, Sánchez-Moreno M Tags: J Inorg Biochem Source Type: research

Assembly behavior of amylin fragment hIAPP19-37 regulated by Au(III) complexes.
Abstract Human islet amyloid polypeptide (hIAPP, amylin) may self-aggregate and rupture the membrane of β cells, which is closely correlated with the pathogenesis of type 2 diabetes mellitus (T2DM). Hence, suppressing amyloidogenic hIAPP may be beneficial for the treatment of diabetes. As an important part of hIAPP, the fragment hIAPP19-37 was studied in this work to explore their disaggregation and cellular behavior regulation by some selected Au complexes, as follows: dichloro diethyl dithiocarbamate Au complex [AuCl2(DDTC)] (1), dichloro pyrrolidine dithiocarbamate Au complex [AuCl2(PDT)] (2), dichloro 4-4...
Source: Journal of Inorganic Biochemistry - August 28, 2019 Category: Biochemistry Authors: Huang X, Xu J, Du W Tags: J Inorg Biochem Source Type: research

Antiproliferative activity of heterometallic sodium and potassium-dioxidovanadium(V) polymers.
Abstract The syntheses of the heterometallic sodium and potassium-dioxidovanadium 2D polymers, [NaVO2(1κNOO';2κO"-L)(H2O)]n(1) and [KVO2(1κNOO';2κO';3κO"-L)(EtOH)]n(2) (where the κ notation indicates the coordinating atoms of the polydentate ligand L) derived from (3,5-di-tert-butyl-2-hydroxybenzylidene)-2-hydroxybenzohydrazide (H2L) are reported. The polymers were characterized by IR, NMR, elemental analysis and single crystal X-ray diffraction analysis. The antiproliferative potential of 1 and 2 was examined towards four human cancer cell lines (ovarian carcinoma, A2...
Source: Journal of Inorganic Biochemistry - August 26, 2019 Category: Biochemistry Authors: Sutradhar M, Alegria ECBA, Ferretti F, Raposo LR, Guedes da Silva MFC, Baptista PV, Fernandes AR, Pombeiro AJL Tags: J Inorg Biochem Source Type: research