Novel method for rapid copper chelation assessment confirmed low affinity of D-penicillamine for copper in comparison with trientine and 8-hydroxyquinolines.
This study was aimed at: 1) formation of an in vitro methodology for screening of copper chelators, and 2) detailed analysis of the interaction of copper with clinically used D-penicillamine (D-PEN), triethylenetetramine (trientine), experimentally tested 8-hydroxyquinolines, and the disodium salt of EDTA as a standard chelator. Methodology based on bathocuproinedisulfonic acid disodium salt (BCS), usable at (patho)physiologically relevant pHs (4.5-7.5), enabled assessment of both cuprous and cupric ions chelation and comparison of the relative affinities of the tested compounds for copper. In the case of potent chelators,...
Source: Journal of Inorganic Biochemistry - May 23, 2013 Category: Biochemistry Authors: Ríha M, Karlíčková J, Filipský T, Macáková K, Hrdina R, Mladěnka P Tags: J Inorg Biochem Source Type: research

Platinum-based drugs and proteins: reactivity and relevance to DNA adduct formation.
Abstract The mechanism of action of clinically used Pt-based drugs is through the formation of stable DNA adducts occurring at the nitrogen in position 7 of guanine (N7) and involving one or two spatially closed residues. Nevertheless, proteins can represent alternative targets since in particular sulfur groups, present in cysteine or methionine residues, can efficiently coordinate platinum. Here we have characterized the reactivity profile of cisplatin, transplatin and of two trans-platinum amine derivatives (TPAs) towards three different proteins, bovine α-lactalbumin (α-LA), hen egg lysozyme (LYS) a...
Source: Journal of Inorganic Biochemistry - May 1, 2013 Category: Biochemistry Authors: Pinato O, Musetti C, Farrell NP, Sissi C Tags: J Inorg Biochem Source Type: research

Complexes of ferriheme nitrophorin 4 with low-molecular weight thiol(ate)s occurring in blood plasma.
p M Abstract Nitrophorins are proteins occurring in the saliva of the blood-sucking insect Rhodnius prolixus to carry NO as a vasodilator and blood-coagulation inhibitor into the victim's tissue. It was suggested that the rate of NO release can be enhanced by the blood-plasma component L-cysteine [J.M.C.Ribeiro, Insect Biochem. Mol. Biol. 26 (1996) 899-905]. However, the mechanism of the reaction is not clear. In the attempt to exploit the reaction in detail, complexes of nitrophorin 4 (NP4) with the thiols 2-mercaptoethanol, L-cysteine, and L-homocysteine and with HS(-) were formed and characterized under anaerob...
Source: Journal of Inorganic Biochemistry - May 1, 2013 Category: Biochemistry Authors: He C, Nishikawa K, Erdem ÖF, Reijerse E, Ogata H, Lubitz W, Knipp M Tags: J Inorg Biochem Source Type: research

Impact of metal on the DNA photo-induced cleavage activity of a family of Phterpy complexes.
Abstract A family of Phterpy complexes, [Mn(Phterpy)2][N(CN)2]2·0.5H2O (1), [Fe(Phterpy)2](NO3)2 (2), [Ni(Phterpy)2](NO3)2 (3), [Ni(Phterpy)2]Cl2·10H2O (4), [Cd(Phterpy)2](NO3)2·2H2O (5) and [Zn(Phterpy)Cl2] (6) (Phterpy=4'-phenyl-2,2':6',2″-terpyridine), have been synthesized and structurally characterized, and their DNA binding and photo-induced DNA cleavage activities have been investigated. These complexes display binding propensity to the CT-DNA giving a relative order: 1>4>3, 5, 2, 6. Under dark or ambient lighting conditions, all complexes show no efficient DNA cleavage acti...
Source: Journal of Inorganic Biochemistry - May 1, 2013 Category: Biochemistry Authors: Chen GJ, Wang ZG, Kou YY, Tian JL, Yan SP Tags: J Inorg Biochem Source Type: research

Novel water-soluble (99m)Tc(I)/Re(I)-porphyrin conjugates as potential multimodal agents for molecular imaging.
Abstract The synthesis and characterization of two novel water soluble porphyrins with three meso pyridyl rings and one peripheral chelator - either a diethylenetriamine unit (4) or a bipyridyl fragment (8) - for binding to the {(99m)Tc(CO)3}(+) moiety is reported. In 8, despite the presence of a flexible and hydrophilic PEG-like linker that connects the bpy unit to the macrocycle, good water solubility was only obtained by methylation of the pyridyl N atoms that provided three extra positive charges. Furthermore, the water-soluble conjugates of 4 and 8 with either one fac-{Re(CO)3}(+) (9 and 10, respectively) or ...
Source: Journal of Inorganic Biochemistry - May 1, 2013 Category: Biochemistry Authors: Spagnul C, Alberto R, Gasser G, Ferrari S, Pierroz V, Bergamo A, Gianferrara T, Alessio E Tags: J Inorg Biochem Source Type: research

Comparative study of reaction of cobalamin and cobinamide with thiocyanate.
Abstract The interaction of Co(III) and Co(II) cobalamin (Cbl) and cobinamide (Cbi) with thiocyanate was examined with UV-vis and EPR spectra. S/N-linkage isomerism was explored on Co(III) and Co(II) Cbl and Cbi models using density functional theory (DFT; BP86, B3LYP). Performed calculations suggest the prevalence of isothiocyanato isomers over thiocyanato complexes on both Co(III) and Co(II) centers. The formation of Cbl(II) complex with thiocyanate was observed at high ligand concentrations which was proposed to be hexacoordinated. DFT data maintain the possibility of hexacoordinated Co(II) complexes with thioc...
Source: Journal of Inorganic Biochemistry - April 28, 2013 Category: Biochemistry Authors: Dereven'kov IA, Salnikov DS, Makarov SV, Surducan M, Silaghi-Dumitrescu R, Boss GR Tags: J Inorg Biochem Source Type: research

The second generation of iodido complexes: trans-[PtI2(amine)(amine')] bearing different aliphatic amines.
Quiroga AG Abstract The antitumoral potential for a series of platinum iodido complexes, all bearing the same aliphatic amines (first iodido complexes generation), was demonstrated in a previous study. Concretely, cis complexes were shown to have a peculiar and different reactivity compared to cisplatin with sulfur donors models and Cyt C. In this work we have synthesized and studied iodido complexes bearing different aliphatic amines in trans configuration (the second generation) to investigate their potential antitumor activity in a panel of cell lines. Their interaction with biological models such as pBR322 an...
Source: Journal of Inorganic Biochemistry - April 28, 2013 Category: Biochemistry Authors: Parro T, Medrano MA, Cubo L, Muñoz-Galván S, Carnero A, Navarro-Ranninger C, Quiroga AG Tags: J Inorg Biochem Source Type: research

Synthesis, chemical characterization and biological activity of new histone acetylation/deacetylation specific inhibitors: A novel and potential approach to cancer therapy.
Abstract Three new triorganotin(IV) complexes of valproic acid (vp1, Me3Sn-valproate; vp2, Bu3Sn-valproate; vp3, Ph3Sn-valproate) have been synthesized and investigated by spectroscopic and biological methods. An anionic, monodentate valproate ligand was observed, ester-like coordinating the tin atom on a tetra-coordinated, monomeric environment. The structures, though, can distort towards a penta-coordination, as a consequence of a long range O···Sn interaction. Crystallographic and NMR findings confirm this situation both in solid state and solution. Biological finding evidenced a clear cyto...
Source: Journal of Inorganic Biochemistry - April 25, 2013 Category: Biochemistry Authors: Pellerito O, Prinzivalli C, Foresti E, Sabatino P, Abbate M, Casella G, Fiore T, Scopelliti M, Pellerito C, Giuliano M, Grasso G, Pellerito L Tags: J Inorg Biochem Source Type: research

Palladium(II) and platinum(II) bis(thiosemicarbazone) complexes of the 2,6-diacetylpyridine series with high cytotoxic activity in cisplatin resistant A2780cisR tumor cells and reduced toxicity.
Abstract Preparation and characterization of four novel 2,6-diacetylpyridine bis((4)N-tolylthiosemicarbazonato) palladium(II) and platinum(II) complexes, [PdL(1--2)] and [PtL(1-2)], are described. All compounds have been characterized by elemental analysis and by IR and NMR spectroscopy, and the crystal and molecular structures of complexes [PdL(2)] and [PtL(2)] have been determined by a single crystal X-ray diffraction. The ligands act as dianionic tetradentate donors coordinating to the metal center in a square planar geometry through the Npyridinic atom and the Niminic and the S atoms from one thiosemicarbazone...
Source: Journal of Inorganic Biochemistry - April 23, 2013 Category: Biochemistry Authors: Matesanz AI, Leitao I, Souza P Tags: J Inorg Biochem Source Type: research

Kinetics and mechanism of reactions of the drug tiopronin with platinum(IV) complexes.
Abstract Tiopronin, a synthetic thiol-containing drug being used in treatments of cystinuria and certain types of rare arthritis, is also a hepatoprotective and a detoxifying agent. Many analytical methods have been developed based on its redox chemistry with metal ions/complexes, but the kinetic and mechanistic aspects are poorly understood. In this work, the oxidation of tiopronin by cisplatin prodrug and a model compound, cis-[Pt(NH3)2Cl4] and trans-[PtCl2(CN)4](2-), was investigated. The oxidation kinetics was followed by a stopped-flow spectrophotometer over a wide pH range under the pseudo first-order condit...
Source: Journal of Inorganic Biochemistry - April 19, 2013 Category: Biochemistry Authors: Huo S, Shi H, Liu D, Shen S, Zhang J, Song C, Shi T Tags: J Inorg Biochem Source Type: research

Bio-inspired citrate-functionalized apatite thin films crystallized on Ti-6Al-4V implants pre-coated with corrosion resistant layers.
ute;mez-Morales J Abstract In this paper the crystallization of a bioinspired citrate-functionalized apatite (cit-Ap) thin film (thickness about 2μm) on Ti-6Al-4V supports pre-coated with bioactive and corrosion resistant buffer layer of silicon nitride (Si3N4), silicon carbide (SiC) or titanium nitride (TiN) is reported. The apatitic coatings were produced by a new coating technique based on the induction heating of the implants immersed in a flowing calcium-citrate-phosphate solution at pH11. The influence of the buffer layers and the surface roughness of the substrate on the chemical-physical features and ad...
Source: Journal of Inorganic Biochemistry - April 18, 2013 Category: Biochemistry Authors: Delgado-López JM, Iafisco M, Rodríguez-Ruiz I, Gómez-Morales J Tags: J Inorg Biochem Source Type: research

The abilities of selenium dioxide and selenite ion to coordinate DNA-bound metal ions and decrease oxidative DNA damage.
Abstract Several transition metals react with H2O2 and produce reactive oxygen species (ROS) responsible for oxidative damage linked to many diseases and disorders, and species that form coordination complexes with these metal ions show promise as antioxidants. The present study demonstrates that metal-mediated radical and non-radical oxidative DNA damage decreases when selenium dioxide (SeO2) and sodium selenite (Na2SeO3) are present. Radical-induced damage is associated with production of 8-hydroxy-2'-deoxyguanosine (8-OH-dG), which arises from ROS generated at or near the guanine base, and the selenium compound...
Source: Journal of Inorganic Biochemistry - April 6, 2013 Category: Biochemistry Authors: Hart WE, Marczak SP, Kneller AR, French RA, Morris DL Tags: J Inorg Biochem Source Type: research

Synthesis, structure and in vitro cytotoxic studies of novel paramagnetic palladium(III) complexes with hematoporphyrin IX.
Abstract Two coordination compounds of Pd(III) with hematoporphyrin IX ((7,12-bis(1-hydroxyethyl)-3,8,13,17-tetramethyl-21H-23H-porphyn-2,18-dipropionic acid), Hp), dinuclear [Pd(III)2(Hp-3H)Cl3(H2O)5]·2PdCl2, 1 and mononuclear [Pd(III)(Hp-2H)Cl(H2O)]·H2O, 2 were obtained and structurally characterized in solid state and solution using spectroscopic, thermal and magnetic methods. In the dinuclear complex, 1 one of the Pd(III) ions is coordinated to the deprotanated COO(-) groups from the side chains of the porphyrin ligand and the second Pd(III) ion - to two adjacent pyrrole N-atoms on the top of the...
Source: Journal of Inorganic Biochemistry - April 2, 2013 Category: Biochemistry Authors: Tsekova D, Gorolomova P, Gochev G, Skumryev V, Momekov G, Momekova D, Gencheva G Tags: J Inorg Biochem Source Type: research

In vitro effects of some gold complexes on Na(+)/K(+) ATPase activity and cell proliferation.
Abstract The in vitro influence of gold(III) complexes, H[AuCl4], [Au(DMSO)2Cl2]Cl and [Au(bipy)Cl2]Cl (bipy = 2,2'-bipyridine), upon commercially available Na(+)/K(+) ATPase activity, purified from porcine brain cortex, was investigated. Additionally, the complexes were tested on human lymphocytes, and incidence of micronuclei and cell proliferation index was determined. Concentration-dependent inhibition of the enzyme for all three compounds was obtained, but with differing potencies. Calculated IC50 from Hill analysis were (in M): 5.75×10(-7), 5.50×10(-6) and 3.98×10(-5), for H[AuCl4], [Au(DMS...
Source: Journal of Inorganic Biochemistry - March 29, 2013 Category: Biochemistry Authors: Petrović V, Colović M, Krstić D, Vujačić A, Petrović S, Joksić G, Bugarčić Z, Vasić V Tags: J Inorg Biochem Source Type: research

Role of liver fatty acid binding protein in hepatocellular injury: Effect of CrPic treatment.
This study was designed to investigate the molecular mechanisms of chromium picolinate (CrPic, Fig. 1) hepatoprotective activity from alloxan-induced hepatic injury. Diabetes is induced by alloxan-treatment concurrently with the hepatic injury in mice. In this study, we investigate the protective effect of CrPic treatment in hepatic injury and the signal role of liver fatty acid binding protein in early hepatocellular injury diagnostics. In this study, alanine aminotransferase (ALT; EC 2.6.1.2) and aspartate aminotransferase (AST; EC 2.6.1.1) levels in the alloxan group were higher 71% and 50%, respectively, than thos...
Source: Journal of Inorganic Biochemistry - March 29, 2013 Category: Biochemistry Authors: Fan W, Chen K, Zheng G, Wang W, Teng A, Liu A, Ming D, Yan P Tags: J Inorg Biochem Source Type: research

Metal complex-DNA binding: Insights from molecular dynamics and DFT/MM calculations.
Abstract Molecular dynamics (MD) simulations, followed by density functional theory/molecular mechanics (DFT/MM) calculations, provided a detailed structure of the binding site between the cationic metallointercalator (dipyrido [3,2-a:2',3'-c]phenazine)(glycinato)copper(II), [Cu(gly)(dppz)](+), and the two dodeca-deoxynucleotide duplexes [dodeca(dG-dC)]2 and [dodeca(dA-dT)]2. Three simultaneous DNA binding types were detected in the fully optimized DFT/MM structures: 1) metal coordination through exocyclic oxygen atoms of nitrogen bases; 2) intercalation of the dppz chromophore between stacked Watson-Crick AT-AT a...
Source: Journal of Inorganic Biochemistry - March 29, 2013 Category: Biochemistry Authors: Spinello A, Terenzi A, Barone G Tags: J Inorg Biochem Source Type: research

A novel platinum complex of the histone deacetylase inhibitor belinostat: Rational design, development and in vitro cytotoxicity.
Abstract The successful design and synthesis of a novel Pt complex of the histone deacteylase inhibitor belinostat are reported. Molecular modelling assisted in the identification of a suitable malonate derivative of belinostat (mal-p-Bel) for complexation to platinum. Reaction of [Pt(NH3)2(H2O)2](NO3)2 with the disodium salt of mal-p-Bel gave cis-[Pt(NH3)2(mal-p-Bel-2H)] (where -2H indicates that mal-p-Bel is doubly deprotonated) in excellent yield. An in vitro cytotoxicity study revealed that cis-[Pt(NH3)2(mal-p-Bel-2H)] possesses (i) considerable cytotoxicity against reported ovarian cancer cell lines, (ii) enh...
Source: Journal of Inorganic Biochemistry - March 29, 2013 Category: Biochemistry Authors: Parker JP, Nimir H, Griffith DM, Duff B, Chubb AJ, Brennan MP, Morgan MP, Egan DA, Marmion CJ Tags: J Inorg Biochem Source Type: research

Synthesis, properties, and antitumor effects of a new mixed phosphine gold(I) compound in human colon cancer cells.
Abstract The antineoplastic potential of a new stable mixed phosphine gold(I) complex containing tris(tert-butyl)phosphine (tBu3P) and bis(diphenylphosphino)ethene (dppet), namely [Au(tBu3P)(dppet)Cl], has been investigated in the human colon cancer HCT-116 cell line. The (31)P NMR solution study, confirms the structural features observed in the solid state and, in addition, indicates partial formation of dinuclear cationic [Au(tBu3P)2](+) and [Au(dppet)2](+) species. The ionic character and strong Au-P bonds of this gold(I) species are similar to those of the most active antitumor gold compounds so far studied. T...
Source: Journal of Inorganic Biochemistry - March 29, 2013 Category: Biochemistry Authors: Lupidi G, Avenali L, Bramucci M, Quassinti L, Pettinari R, Khalife HK, Gali-Muhtasib H, Marchetti F, Pettinari C Tags: J Inorg Biochem Source Type: research

A QM/MM refinement of an experimental DNA structure with metal-mediated base pairs.
r J Abstract A series of hybrid quantum mechanical/molecular mechanical (QM/MM) calculations was performed on models of a DNA duplex with artificial silver(I)-mediated imidazole base pairs. The optimized structures were compared to the original experimental NMR structure (Nat. Chem. 2 (2010) 229-234). The metal⋯metal distances are significantly shorter (~0.5Å) in the QM/MM model than in the original NMR structure. As a result, argentophilic interactions are feasible between the silver(I) ions of neighboring metal-mediated base pairs. Using the computationally determined metal⋯metal distances, a re-refine...
Source: Journal of Inorganic Biochemistry - March 28, 2013 Category: Biochemistry Authors: Kumbhar S, Johannsen S, Sigel RK, Waller MP, Müller J Tags: J Inorg Biochem Source Type: research

Dissecting the kinetics of the NADP(+)-FADH2 charge transfer complex and flavin semiquinones in neuronal nitric oxide synthase.
Abstract Electron flow within the neuronal nitric oxide synthase reductase domain (nNOSrd) includes hydride transfer from NADPH to FAD followed by two one-electron transfer reactions from FAD to FMN. We have used stopped flow spectrometry to closely monitor these electron transfer steps for both the wild type and the ΔG810 mutant of nNOSrd using a protocol involving both global analyses of the photodiode array spectral scans and curve fittings of single wavelength kinetic traces. The charge transfer complex and interflavin electron transfer events recorded at 750nm and 600nm, respectively, show the kinetics ...
Source: Journal of Inorganic Biochemistry - March 22, 2013 Category: Biochemistry Authors: Li H, Jamal J, Chreifi G, Venkatesh V, Abou-Ziab H, Poulos TL Tags: J Inorg Biochem Source Type: research

Evaluation of the binding of polyhedral borane anions to representative proteins.
Abstract The ability of three polyhedral borane anions, [B20H18](2-), [B20H17SH](4-), and [B20H19](3-), to bind to proteins was evaluated by measuring the total boron content using inductively coupled plasma-atomic emission spectroscopy after purification by gel electrophoresis. Results were correlated to the known chemical reactivity of the compounds as well as the reported murine biodistributions of the liposomally encapsulated sodium salts of each of the polyhedral borane anions. Qualitative reactions were performed with the [B20H18](2-) anion to determine the potential reactivity with simple molecular building...
Source: Journal of Inorganic Biochemistry - March 21, 2013 Category: Biochemistry Authors: Waller RC, Booth RE, Feakes DA Tags: J Inorg Biochem Source Type: research

Mono- and bis-phosphine-ligated H93G myoglobin: Spectral models for ferrous-phosphine and ferrous-CO cytochrome P450.
This study also further demonstrates the utility of the H93G cavity mutant for preparing novel heme iron coordination structures. PMID: 23639797 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - March 21, 2013 Category: Biochemistry Authors: Sun S, Sono M, Dawson JH Tags: J Inorg Biochem Source Type: research

Direct interactions between Z-DNA and alkaline earth cations, discovered in the presence of high concentrations of MgCl2 and CaCl2.
In this study, crystals of Z-DNA hexamer d(CGCGCG) complexed with MgCl2 and CaCl2 were obtained in the presence of high concentrations of alkaline earth salts (500mM) using a temperature control technique, and their crystal structures were determined at 1.3Å resolution. Mg(2+) and Ca(2+) cations in these structures tend to interact directly with phosphate groups of Z-DNA duplexes; however, they tend to form water-mediated interactions with Z-DNA in the presence of lower concentrations of alkaline earth salts. In these crystals, a DNA duplex was laid along its c-axis and interacted with its 6 neighboring DNA duplexes ...
Source: Journal of Inorganic Biochemistry - March 19, 2013 Category: Biochemistry Authors: Chatake T, Sunami T Tags: J Inorg Biochem Source Type: research

Size matters, so does shape: Inhibition of transcription of T7 RNA polymerase by iron(II) clathrochelates.
Abstract Coordination and organoelement compounds are rarely proposed as the drug candidates despite their vast potential in the area owing to their strictly controlled geometry and rather extensive surface. This is the first example of the inhibition of transcription in the system of T7 RNA polymerase by cage metal complexes. Their IC50 values reach as low as the nanomolar range, placing them among the most potent metal-based transcription inhibitors. PMID: 23598064 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - March 19, 2013 Category: Biochemistry Authors: Novikov VV, Varzatskii OA, Negrutska VV, Bubnov YN, Palchykovska LG, Dubey IY, Voloshin YZ Tags: J Inorg Biochem Source Type: research

High affinity of copper(II) towards amoxicillin, apramycin and ristomycin. Effect of these complexes on the catalytic activity of HDV ribozyme.
zuk M Abstract Three representatives of the distinct antibiotics groups: amoxicillin, apramycin and ristomycin A were studied regarding their impact on hepatitis D virus (HDV) ribozyme both in the metal-free form and complexed with copper(II) ions. Hence the Cu(II)-ristomycin A complex has been characterized by means of NMR, EPR, CD and UV-visible spectroscopic techniques and its binding pattern has been compared with the coordination modes estimated previously for Cu(II)-amoxicillin and Cu(II)-apramycin complexes. It has thus been found that all three antibiotics bind the Cu(II) ion in a very similar manner, enga...
Source: Journal of Inorganic Biochemistry - March 16, 2013 Category: Biochemistry Authors: Stokowa-Sołtys K, Gaggelli N, Nagaj J, Szczepanik W, Ciesiołka J, Wrzesiński J, Górska A, Gaggelli E, Valensin G, Jeżowska-Bojczuk M Tags: J Inorg Biochem Source Type: research

New bifunctional metalloproteinase inhibitors: an integrated approach towards biological improvements and cancer therapy.
We present herein an integrated approach for the development of a new series of inhibitors of MMP2 and MMP14, two enzymes over-expressed by human ovarian cancer. As a first step, a new series of single model compounds bearing different zinc-binding groups (ZBGs), such as carboxylic, hydroxamic acid, hydrazide and sulfonylhydrazide groups, were studied and revealed reasonably good capacity for the Zn(II) chelation in solution and for the MMP inhibition. Aimed at further reinforcing the biological activity of these MMPIs as anti-cancer agents, a selection of those models was extra-functionalized with benzothiazole (BTA), a g...
Source: Journal of Inorganic Biochemistry - March 16, 2013 Category: Biochemistry Authors: Marques SM, Abate CC, Chaves S, Marques F, Santos I, Nuti E, Rossello A, Santos MA Tags: J Inorg Biochem Source Type: research

Oxidovanadium(IV) and dioxidovanadium(V) complexes of tridentate salicylaldehyde semicarbazones: Searching for prospective antitrypanosomal agents.
arat B, Gambino D Abstract As a contribution to the identification of the relevant species for biological activity and the understanding of structure-activity relationships of [V(IV)O(L-2H)(NN)] antitrypanosomal complexes (NN is a bidentate polypyridyl DNA intercalator; L is a tridentate salicylaldehyde semicarbazone derivative), new [V(V)O2(L-2H)] complexes and [V(IV)O(L-2H)(NN)] complexes including bipy or dppz (dipyrido[3,2-a: 2',3'-c]phenazine) co-ligands are prepared and characterized in the solid state and in solution. Their activity is evaluated on Trypanosoma cruzi. The lipophilicity, as structural descrip...
Source: Journal of Inorganic Biochemistry - March 4, 2013 Category: Biochemistry Authors: Fernández M, Becco L, Correia I, Benítez J, Piro OE, Echeverria GA, Medeiros A, Comini M, Lavaggi ML, González M, Cerecetto H, Moreno V, Pessoa JC, Garat B, Gambino D Tags: J Inorg Biochem Source Type: research

Structural insights on the molecular recognition patterns between N(6)-substituted adenines and N-(aryl-methyl)iminodiacetate copper(II) chelates.
tilde;eiras A, Niclós-Gutiérrez J Abstract For a better understanding of the metal binding pattern of N(6)-substituted adenines, six novel ternary Cu(II) complexes have been structurally characterized by single crystal X-ray diffraction: [Cu(NBzIDA)(HCy5ade)(H2O)]·H2O (1), [Cu(NBzIDA)(HCy6ade)(H2O)]·H2O (2), [Cu(FurIDA)(HCy6ade)(H2O)]·H2O (3), [Cu(MEBIDA)(HBAP)(H2O)]·H2O (4), [Cu(FurIDA)(HBAP)]n (5) and {[Cu(NBzIDA)(HdimAP)]·H2O}n (6). In these compounds NBzIDA, FurIDA and MEBIDA are N-substituted iminodiacetates with a non-coordinating aryl-methyl pendant arm (benz...
Source: Journal of Inorganic Biochemistry - February 15, 2013 Category: Biochemistry Authors: Domínguez-Martín A, García-Raso A, Cabot C, Choquesillo-Lazarte D, Pérez-Toro I, Matilla-Hernández A, Castiñeiras A, Niclós-Gutiérrez J Tags: J Inorg Biochem Source Type: research

Expanding the synthesis of new trans-sulfonamide platinum complexes: Cytotoxicity, SAR, fluorescent cell assays and stability studies.
avarro-Ranninger C, Alemán J Abstract In this manuscript, we describe the synthesis of new trans-N-sulfonamide platinum complexes and their antiproliferative activity (GI50, μM) in human solid tumors cells. The structure activity relationships (SAR), with different new synthesized complexes by variation in ligand, halogen and also in the stereochemistry of the ligand, has been studied. Solubility and stability studies have also been carried out as well as fluorescent cell assays in order to clarify the final target in the tumor cells. PMID: 23474039 [PubMed - as supplied by publisher] (Source: Journ...
Source: Journal of Inorganic Biochemistry - February 8, 2013 Category: Biochemistry Authors: Del Solar V, Quiñones-Lombraña A, Cabrera S, Padrón JM, Ríos-Luci C, Alvarez-Valdés A, Navarro-Ranninger C, Alemán J Tags: J Inorg Biochem Source Type: research