Solution structures of chloroquine-ferriheme complexes modeled using MD simulation and investigated by EXAFS spectroscopy.
Abstract The interaction of chloroquine (CQ) and the μ-oxo dimer of iron(III) protoporphyrin IX (ferriheme) in aqueous solution was modeled using molecular dynamics (MD) simulations. Two models of the CQ-(μ-oxo ferriheme) complex were investigated, one involving CQ π-stacked with an unligated porphyrin face of μ-oxo ferriheme and the other in which CQ was docked between the two porphyrin rings. The feasibility of both models was tested by fitting computed structures to the experimental extended X-ray absorption fine structure (EXAFS) spectrum of the CQ-(μ-oxo ferriheme) complex in frozen aqueous sol...
Source: Journal of Inorganic Biochemistry - June 11, 2015 Category: Biochemistry Authors: Kuter D, Streltsov V, Davydova N, Venter GA, Naidoo KJ, Egan TJ Tags: J Inorg Biochem Source Type: research

Recent advances on antimony(III/V) compounds with potential activity against tumor cells.
Abstract Antimony one of the heavier pnictogens, has been in medical use against microbes and parasites as well. Antimony-based drugs have been prescribed against leishmaniasis since the parasitic transmission of the tropical disease was understood in the beginning of the 20th century. The activity of arsenic against visceral leishmaniasis led to the synthesis of an array of arsenic-containing parasitic agents, among them the less toxic pentavalent antimonials: Stibosan, Neostibosan, and Ureastibamine. Other antimony drugs followed: sodium stibogluconate (Pentostam) and melglumine antimoniate (Glucantim or Glucant...
Source: Journal of Inorganic Biochemistry - June 10, 2015 Category: Biochemistry Authors: Hadjikakou SK, Ozturk II, Banti CN, Kourkoumelis N, Hadjiliadis N Tags: J Inorg Biochem Source Type: research

Antitumor and antiparasitic activity of novel ruthenium compounds with polycyclic aromatic ligands.
Abstract Five novel ruthenium(II)-arene complexes with polycyclic aromatic ligands were synthesized, comprising three compounds of the formula [RuCl(η(6)-p-cym)(L)][PF6], where p-cym=1-isopropyl-4-methylbenzene and L are the bidentate aromatic ligands 1,10-phenanthroline-5,6-dione, 1, 5-amine-1,10-phenanthroline, 4, or 5,6-epoxy-5,6-dihydro-phenanthroline, 5. In the other two complexes [RuCl2(η(6)-p-cym)(L')], the metal is coordinated to a monodentate ligand L', where L' is phenanthridine, 2, or 9-carbonylanthracene, 3. All compounds were fully characterized by mass spectrometry and elemental analysis, as ...
Source: Journal of Inorganic Biochemistry - June 9, 2015 Category: Biochemistry Authors: Miserachs HG, Cipriani M, Grau J, Vilaseca M, Lorenzo J, Medeiros A, Comini MA, Gambino D, Otero L, Moreno V Tags: J Inorg Biochem Source Type: research

Molecular mechanisms of apoptosis and cell selectivity of zinc dithiocarbamates functionalized with hydroxyethyl substituents.
Abstract In the solid state each of three binuclear zinc dithiocarbamates bearing hydroxyethyl groups, {Zn[S2CN(R)CH2CH2OH]2}2 for R=iPr (1), CH2CH2OH (2), and Me (3), and an all alkyl species, [Zn(S2CNEt2)2]2 (4), features a centrosymmetric {ZnSCS}2 core with a step topology; both 1 and 3 were isolated as monohydrates. All compounds were broadly cytotoxic, specifically against human cancer cell lines compared with normal cells, with greater potency than cisplatin. Notably, some selectivity were indicated with 2 being the most potent against human ovarian carcinoma cells (cisA2780), and 4 being more cytotoxic towa...
Source: Journal of Inorganic Biochemistry - June 9, 2015 Category: Biochemistry Authors: Tan YS, Ooi KK, Ang KP, Akim AM, Cheah YK, Halim SN, Seng HL, Tiekink ER Tags: J Inorg Biochem Source Type: research

Synthesis, chemical characterization, computational studies and biological activity of new DNA methyltransferases (DNMTs) specific inhibitor. Epigenetic regulation as a new and potential approach to cancer therapy.
Abstract This work deals with the synthesis, the chemical characterization of dibutyltin(IV) complex of caffeic acid (Bu2Sn(IV)HCAF, caf1) and its cytotoxic action on tumor cells. The coordination environment at the tin center was investigated by FTIR, (119)Sn{(1)H} cross polarization magic angle spinning, electrospray ionization mass spectroscopy in the solid state and UV-vis, fluorescence and (1)H, (13)C and (119)Sn NMR spectroscopy in solution phases. Density functional theory study confirmed the proposed structures in solution phase and indicated the most probably stable conformation. The effects on viability ...
Source: Journal of Inorganic Biochemistry - June 5, 2015 Category: Biochemistry Authors: Pellerito C, Morana O, Ferrante F, Calvaruso G, Notaro A, Sabella S, Fiore T Tags: J Inorg Biochem Source Type: research

Synthesis, crystal structure and biological activity of two Mn complexes with 4-acyl pyrazolone derivatives.
Abstract In order to study the biological activities of transitional metal complexes based on 4-acyl pyrazolone derivatives, two Mn complexes [Mn(HLa)(La)]·(CH3CN)1.5·H2O (1) and [Mn2(Lb)2(μ-EtO)2(EtOH)2] (2) (H2La=N-(1-phenyl-3-methyl-4-benzoyl-5-pyrazolone)-2-thiophenecarboxylic acid hydrazide, H2Lb=N-(1-phenyl-3-methyl-4-propenylidene-5-pyrazolone)-2-thiophenecarboxylic acid hydrazide) have been synthesized and characterized. Single crystal X-ray diffraction analysis indicated that 1 is a mononuclear complex and 2 exhibits a dinuclear centrosymmetric structure. Binding of the complexes with Her...
Source: Journal of Inorganic Biochemistry - June 5, 2015 Category: Biochemistry Authors: Li Y, Zhao J, He CC, Zhang L, Sun SR, Xu GC Tags: J Inorg Biochem Source Type: research

A versatile salicyl hydrazonic ligand and its metal complexes as antiviral agents.
Abstract Acylhydrazones are very versatile ligands and their coordination properties can be easily tuned, giving rise to metal complexes with different nuclearities. In the last few years, we have been looking for new pharmacophores able to coordinate simultaneously two metal ions, because many enzymes have two metal ions in the active site and their coordination can be a successful strategy to inhibit the activity of the metalloenzyme. As a part of this ongoing research, we synthesized the acylhydrazone H2L and its complexes with Mg(II), Mn(II), Co(II), Ni(II), Cu(II) and Zn(II). Their characterization, both in s...
Source: Journal of Inorganic Biochemistry - May 27, 2015 Category: Biochemistry Authors: Rogolino D, Carcelli M, Bacchi A, Compari C, Contardi L, Fisicaro E, Gatti A, Sechi M, Stevaert A, Naesens L Tags: J Inorg Biochem Source Type: research

Cellular trafficking, accumulation and DNA platination of a series of cisplatin-based dicarboxylato Pt(IV) prodrugs.
Abstract A series of Pt(IV) anticancer prodrug candidates, having the equatorial arrangement of cisplatin and bearing two aliphatic carboxylato axial ligands, has been investigated to prove the relationship between lipophilicity, cellular accumulation, DNA platination and antiproliferative activity on the cisplatin-sensitive A2780 ovarian cancer cell line. Unlike cisplatin, no facilitated influx/efflux mechanism appears to operate in the case of the Pt(IV) complexes under investigation, thus indicating that they enter by passive diffusion. While Pt(IV) complexes having lipophilicity comparable to that of cisplatin...
Source: Journal of Inorganic Biochemistry - May 27, 2015 Category: Biochemistry Authors: Ravera M, Gabano E, Zanellato I, Bonarrigo I, Alessio M, Arnesano F, Galliani A, Natile G, Osella D Tags: J Inorg Biochem Source Type: research

Impact of Cu(2+) ions on the structure of colistin and cell-free system nucleic acid degradation.
Abstract Colistin and transition metal ions are commonly used as feed additives for livestock animals. This work presents the results of an analysis of combined potentiometric and spectroscopic (UV-vis, EPR, CD, NMR) data which lead to conclude that colistin is able to effectively chelate copper(II) ions. In cell-free system the oxidative activity of the complex manifests itself in the plasmid DNA destruction with simultaneous generation of reactive OH species, when accompanied by hydrogen peroxide or ascorbic acid. The degradation of RNA occurs most likely via a hydrolytic mechanism not only for complexed compoun...
Source: Journal of Inorganic Biochemistry - May 27, 2015 Category: Biochemistry Authors: Stokowa-Sołtys K, Kasprowicz A, Wrzesiński J, Ciesiołka J, Gaggelli N, Gaggelli E, Valensin G, Jeżowska-Bojczuk M Tags: J Inorg Biochem Source Type: research

Reactivity of dinuclear copper(II) complexes towards melanoma cells: Correlation with its stability, tyrosinase mimicking and nuclease activity.
Ferreira AM Abstract In this work, the influence of two new dinuclear copper(II) complexes in the viability of melanoma cells (B16F10 and TM1MNG3) was investigated, with the aim of verifying possible correlations between their cytotoxicity and their structure. One of the complexes had a polydentate dinucleating amine-imine ligand (complex 2), and the other a tridentate imine and a diamine-bridging ligand (complex 4). The analogous mononuclear copper(II) species (complexes 1 and 3, respectively) were also prepared for comparative studies. Crystal structure determination of complex 2 indicated a square-based pyramid...
Source: Journal of Inorganic Biochemistry - May 19, 2015 Category: Biochemistry Authors: Nunes CJ, Borges BE, Nakao LS, Peyroux E, Hardré R, Faure B, Réglier M, Giorgi M, Prieto MB, Oliveira CC, Da Costa Ferreira AM Tags: J Inorg Biochem Source Type: research

Oxidovanadium(IV) sulfate-induced glucose uptake in HepG2 cells through IR/Akt pathway and hydroxyl radicals.
Abstract The insulin-mimetic and anti-diabetic properties of vanadium and related compounds have been well documented both in vitro and in vivo. However, the molecular basis of the link between vanadium and the insulin signaling pathway in diabetes mellitus is not fully described. We investigated the effects of reactive oxygen species (ROS) induced by oxidovanadium(IV) sulfate (VOSO4) on glucose uptake and the insulin signaling pathway in human hepatoma cell line HepG2. Exposure of cells to VOSO4 (5-50μM) resulted in an increase in glucose uptake, insulin receptor (IR) and protein kinase B (Akt) phosphorylation...
Source: Journal of Inorganic Biochemistry - May 15, 2015 Category: Biochemistry Authors: Zhao Q, Chen D, Liu P, Wei T, Zhang F, Ding W Tags: J Inorg Biochem Source Type: research

Reduced accumulation of platinum drugs is not observed in drug-resistant ovarian cancer cell lines derived from cisplatin-treated patients.
We examined a series of paired cell lines derived from patients with ovarian cancer prior to chemotherapy (PEO1, PEO4, PEO14 and PEA1), and following the acquisition of resistance to a platinum-based chemotherapy regimen (PEO6, PEO23 and PEA2, respectively). All resistant patient lines showed resistance to cisplatin (2-5-fold), but this did not correspond with lowered accumulation. No general cross-resistance was observed for oxaliplatin, paclitaxel or docetaxel, and paclitaxel accumulation was not affected. PEO1 cells carrying BRCA2 mutations were hypersensitive to the PARP inhibitors olaparib and velaparib, but all other...
Source: Journal of Inorganic Biochemistry - May 14, 2015 Category: Biochemistry Authors: Stukova M, Hall MD, Tsotsoros SD, Madigan JP, Farrell NP, Gottesman MM Tags: J Inorg Biochem Source Type: research

Mixed copper-platinum complex formation could explain synergistic antiproliferative effect exhibited by binary mixtures of cisplatin and copper-1,10-phenanthroline compounds: An ESI-MS study.
Abstract Cisplatin, cis-diammineplatinum(II) dichloride, is a metal complex used in clinical practice for the treatment of cancer. Despite its great efficacy, it causes adverse reactions and most patients develop a resistance to cisplatin. To overcome these issues, a multi-drug therapy was introduced as a modern approach to exploit the drug synergy. A synergistic effect had been previously found when testing binary combinations of cisplatin and three copper complexes in vitro, namely, Cu(phen)(OH2)2(OClO3)2, [Cu(phen)2(OH2)](ClO4)2 and [Cu(phen)2(H2dit)](ClO4)2,(phen=1,10-phenanthroline, H2dit=imidazolidine-2-thio...
Source: Journal of Inorganic Biochemistry - May 14, 2015 Category: Biochemistry Authors: Pivetta T, Lallai V, Valletta E, Trudu F, Isaia F, Perra D, Pinna E, Pani A Tags: J Inorg Biochem Source Type: research

Mg(II) and Ni(II) induce aggregation of poly(rA)poly(rU) to either tetra-aggregate or triplex depending on the metal ion concentration.
ini M Abstract The ability of magnesium(II) and nickel(II) to induce dramatic conformational changes in the synthetic RNA poly(rA)poly(rU) has been investigated. Kinetic experiments, spectrofluorometric titrations, melting experiments and DSC measurements contribute in shedding light on a complex behaviour where the action of metal ions (Na(+), Mg(2+), Ni(2+)), in synergism with other operators as the intercalating dye coralyne and temperature, all concur in stabilising a peculiar RNA form. Mg(2+) and Ni(2+) (M) bind rapidly and almost quantitatively to the duplex (AU) to give a RNA/metal ion complex (AUM). Then, ...
Source: Journal of Inorganic Biochemistry - May 9, 2015 Category: Biochemistry Authors: Biver T, Busto N, García B, Leal JM, Menichetti L, Secco F, Venturini M Tags: J Inorg Biochem Source Type: research

Magnetic susceptibility of Mn(III) complexes of hydroxamate siderophores.
This study directly confirms the Mn(III) oxidation state in hydroxamate siderophore complexes. PMID: 25978931 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - May 9, 2015 Category: Biochemistry Authors: Springer SD, Butler A Tags: J Inorg Biochem Source Type: research

Tuning the interactions of PEG-coated gold nanorods with BSA and model proteins through insertion of amino or carboxylate groups.
Abstract Gold nanorods (GNRs) are important platforms for biosensing and drug delivery. As for most nanomaterials, appropriate coatings such as polyethylene glycol (PEG) are needed to stabilize GNRs within biological fluids. We show here that the interactions of GNRs with proteins can be finely modulated through surface modification using PEG-containing chains bearing charged headgroups. Interestingly, introduction of amino or carboxylate groups produces relevant and differential changes in GNR interactions with three representative proteins: lysozyme, cytochrome c, and bovine serum albumin. These effects were exp...
Source: Journal of Inorganic Biochemistry - May 6, 2015 Category: Biochemistry Authors: Scaletti F, Feis A, Centi S, Pini R, Rotello VM, Messori L Tags: J Inorg Biochem Source Type: research

Synthesis, crystal structure and investigation of mononuclear copper(II) and zinc(II) complexes of a new carboxylate rich tripodal ligand and their interaction with carbohydrates in alkaline aqueous solution.
Abstract A new carboxylate rich asymmetric tripodal ligand, N-[2-carboxybenzomethyl]-N-[carboxymethyl]-β-alanine (H3camb), and its di-copper(II), (NH4)2[1]2, and di-zinc(II), ((CH3)4N)2[2]2, complexes have been synthesized as carbohydrate binding models in aqueous solutions. The ligand and complexes have been fully characterized using several techniques, including single crystal X-ray diffraction. The interactions of (NH4)2[1]2 and ((CH3)4N)2[2]2 with d-glucose, d-mannose, d-xylose and xylitol in aqueous alkaline media were investigated using UV-Vis and (13)C-NMR spectroscopic techniques, respectively. The mo...
Source: Journal of Inorganic Biochemistry - April 30, 2015 Category: Biochemistry Authors: Stewart CD, Pedraza M, Arman H, Fan HJ, Schilling EL, Szpoganicz B, Musie GT Tags: J Inorg Biochem Source Type: research

Inhibitory effects of nitrite on the reactions of bovine carbonic anhydrase II with CO2 and bicarbonate consistent with zinc-bound nitrite.
Abstract Carbonic anhydrase (CA) is a zinc enzyme that catalyzes hydration of carbon dioxide (CO2) and dehydration of bicarbonate in red blood cells, thus facilitating CO2 transport and excretion. Bovine CA II may also react with nitrite to generate nitric oxide, although nitrite is a known inhibitor of the CO2 hydration reaction. To address the potential in vivo interference of these reactions and the nature of nitrite binding to the enzyme, we here investigate the inhibitory effect of 10-30mM nitrite on Michaelis-Menten kinetics of CO2 hydration and bicarbonate dehydration by stopped-flow spectroscopy. Our data ...
Source: Journal of Inorganic Biochemistry - April 27, 2015 Category: Biochemistry Authors: Nielsen PM, Fago A Tags: J Inorg Biochem Source Type: research

Synthesis and luminescence properties of salicylaldehyde isonicotinoyl hydrazone derivatives and their europium complexes.
Abstract Four novel salicylaldehyde isonicotinoyl hydrazone derivatives and their corresponding europium ion complexes were synthesized and characterized, while the luminescence properties and the fluorescence quantum yields of the target complexes were investigated. The results indicated that the ligands favored energy transfers to the emitting energy level of europium ion, and four target europium complexes showed the characteristic luminescence of central europium ion. Besides the luminescence intensity of the complex with methoxy group, which possessed the highest fluorescence quantum yield (0.522), was strong...
Source: Journal of Inorganic Biochemistry - April 19, 2015 Category: Biochemistry Authors: Shan W, Liu F, Liu J, Chen Y, Yang Z, Guo D Tags: J Inorg Biochem Source Type: research

Photoinduced reduction of the medial FeS center in the hydrogenase small subunit HupS from Nostoc punctiforme.
n A Abstract The small subunit from the NiFe uptake hydrogenase, HupSL, in the cyanobacterium Nostoc punctiforme ATCC 29133, has been isolated in the absence of the large subunit (P. Raleiras, P. Kellers, P. Lindblad, S. Styring, A. Magnuson, J. Biol. Chem. 288 (2013) 18,345-18,352). Here, we have used flash photolysis to reduce the iron-sulfur clusters in the isolated small subunit, HupS. We used ascorbate as electron donor to the photogenerated excited state of Ru(II)-trisbipyridine (Ru(bpy)3), to generate Ru(I)(bpy)3 as reducing agent. Our results show that the isolated small subunit can be reduced by the Ru(I)...
Source: Journal of Inorganic Biochemistry - April 14, 2015 Category: Biochemistry Authors: Raleiras P, Hammarström L, Lindblad P, Styring S, Magnuson A Tags: J Inorg Biochem Source Type: research

Complexes of N-hydroxyethyl-N-benzimidazolylmethylethylenediaminediacetic acid with group 12 metals and vanadium-Synthesis, structure and bioactivity of the vanadium complex.
Abstract Four new complexes of group 12 metals [Zn(II), Cd(II) and Hg(II)], along with vanadyl bound to the ligand N-hydroxyethyl-N-benzimidazolylmethylethylenediaminediacetic acid, have been synthesized and characterized. The structure of the complexes with Zn(II), Hg(II) and V(IV) was determined by X-ray structural analysis. In all observed cases, the symmetry of these complexes was found to be distorted octahedral. The inhibition of protein tyrosine phosphatase 1B by the vanadium(IV) complex was demonstrated. The cytotoxicity of the vanadium(IV) complex was tested in vitro against three cancer cell lines, with ...
Source: Journal of Inorganic Biochemistry - April 11, 2015 Category: Biochemistry Authors: Habala L, Bartel C, Giester G, Jakupec MA, Keppler BK, Rompel A Tags: J Inorg Biochem Source Type: research

Hypoglycemic, lipid-lowering and metabolic regulation activities of metforminium decavanadate (H2Metf)3 [V10O28]·8H2O using hypercaloric-induced carbohydrate and lipid deregulation in Wistar rats as biological model.
Hypoglycemic, lipid-lowering and metabolic regulation activities of metforminium decavanadate (H2Metf)3 [V10O28]·8H2O using hypercaloric-induced carbohydrate and lipid deregulation in Wistar rats as biological model. J Inorg Biochem. 2015 Apr 10; Authors: Treviño S, Sánchez-Lara E, Sarmiento-Ortega VE, Sánchez-Lombardo I, Flores-Hernández JÁ, Pérez-Benítez A, Brambila-Colombres E, González-Vergara E Abstract Because of the increasing global spread of type 2 diabetes mellitus, there is a need to develop new antidiabetic agents. Recently we ...
Source: Journal of Inorganic Biochemistry - April 10, 2015 Category: Biochemistry Authors: Treviño S, Sánchez-Lara E, Sarmiento-Ortega VE, Sánchez-Lombardo I, Flores-Hernández JÁ, Pérez-Benítez A, Brambila-Colombres E, González-Vergara E Tags: J Inorg Biochem Source Type: research

The bacteriohemerythrin from Methylococcus capsulatus (Bath): Crystal structures reveal that Leu114 regulates a water tunnel.
Abstract The bacteriohemerythrin (McHr) from Methylococcus capsulatus (Bath) is an oxygen carrier that serves as a transporter to deliver O2 from the cytosol of the bacterial cell body to the particulate methane monooxygenase residing in the intracytoplasmic membranes for methane oxidation. Here we report X-ray protein crystal structures of the recombinant wild type (WT) McHr and its L114A, L114Y and L114F mutants. The structure of the WT reveals a possible water tunnel in the McHr that might be linked to its faster autoxidation relative to hemerythrin in marine invertebrates. With Leu114 positioned at the end of ...
Source: Journal of Inorganic Biochemistry - April 10, 2015 Category: Biochemistry Authors: Chen KH, Chuankhayan P, Wu HH, Chen CJ, Fukuda M, Yu SS, Chan SI Tags: J Inorg Biochem Source Type: research

HSA-based phosphorescent probe for two-photon in vitro visualization.
Abstract Two-photon microscopy reveals several advantages over conventional one since it provides higher spatial resolution as well as deeper penetration into the sample under study. The development of suitable two-photon probes is one of the most challenging tasks in this area. Here we present phosphorescent non-covalent adduct of human serum albumin and Au-Ag alkynyl-diphosphine complex, [Au14Ag4(C2Ph)12(PPh2C6H4PPh2)6][PF6]4, which exhibits high cross section of two-photon-induced luminescence (δTPE) within large near-infrared excitation wavelength region (700-800nm) with maximum δTPE about 38 GM at...
Source: Journal of Inorganic Biochemistry - April 1, 2015 Category: Biochemistry Authors: Chelushkin PS, Nukolova NV, Melnikov AS, Serdobintsev PY, Melnikov PA, Krupenya DV, Koshevoy IO, Burov SV, Tunik SP Tags: J Inorg Biochem Source Type: research

Reactions of model proteins with aurothiomalate, a clinically established gold(I) drug: The comparison with auranofin.
Abstract Aurothiomalate (AuTm) is an old, clinically established, antiarthritic gold drug that is currently being reconsidered as a candidate drug for cancer treatment and for other therapeutic indications within a more general drug repositioning program. As the biological effects of gold drugs seem to be mediated, mainly, by their interactions with protein targets we have analyzed here, in detail, the metalation patterns produced by aurothiomalate in a few model proteins. In particular, the reactions of aurothiomalate with the small proteins ribonuclease A, cytochrome c and lysozyme were explored through ESI MS (...
Source: Journal of Inorganic Biochemistry - April 1, 2015 Category: Biochemistry Authors: Darabi F, Marzo T, Massai L, Scaletti F, Michelucci E, Messori L Tags: J Inorg Biochem Source Type: research

Eu(III) luminescence and tryptophan fluorescence spectroscopy as a tool for understanding interactions between hen egg white lysozyme and metal-substituted Keggin type polyoxometalates.
Abstract The interaction between the lacunary Keggin K7PW11O39, the Eu(III)-substituted Keggin K4EuPW11O39 (Eu-Keggin) and the Ce(IV)-substituted Keggin [Me2NH2]10[Ce(PW11O39)2] (Ce-Keggin) polyoxometalates (POMs), and the proteins hen egg white lysozyme (HEWL) and the structurally homologous α-lactalbumin (α-LA) was studied by steady state and time-resolved Eu(III) luminescence and tryptophan (Trp) fluorescence spectroscopy. The excitation spectrum of Eu-Keggin at lower concentrations ([Eu-Keggin]250μM) the (5)L6←(7)F0 transition becomes the most intense peak. In the absence of protein, the nu...
Source: Journal of Inorganic Biochemistry - April 1, 2015 Category: Biochemistry Authors: Goovaerts V, Stroobants K, Absillis G, Parac-Vogt TN Tags: J Inorg Biochem Source Type: research

Caged oligonucleotides for studying biological systems.
Abstract Light-activated ("caged") compounds have been widely employed for studying biological processes with high spatial and temporal control. In the past decade, several new approaches for caging the structure and function of DNA and RNA oligonucleotides have been developed. This review focuses on caged oligonucleotides that incorporate site-specifically one or two photocleavable linkers, whose photolysis yields oligonucleotides with dramatic structural and functional changes. This technique has been employed by our laboratory and others to photoregulate gene expression in cells and living organisms, ...
Source: Journal of Inorganic Biochemistry - March 28, 2015 Category: Biochemistry Authors: Ruble BK, Yeldell SB, Dmochowski IJ Tags: J Inorg Biochem Source Type: research

Iron diminishes the in vitro biological effect of vanadium.
Abstract Mechanistic pathways underlying inflammatory injury following exposures to vanadium-containing compounds are not defined. We tested the postulate that the in vitro biological effect of vanadium results from its impact on iron homeostasis. Human bronchial epithelial (HBE) cells exposed to vanadyl sulfate (VOSO4) showed a time- and dose-dependent increase in vanadium relative to PBS. HBE cells exposed to VOSO4 and then exposed to ferric ammonium citrate (FAC) significantly increased intracellular iron import supporting an interaction between the two metals. Following exposure to VOSO4, there was an increase...
Source: Journal of Inorganic Biochemistry - March 25, 2015 Category: Biochemistry Authors: Ghio AJ, Stonehuerner J, Soukup JM, Dailey LA, Kesic MJ, Cohen MD Tags: J Inorg Biochem Source Type: research

Thirty years through vanadium chemistry.
This article was compiled based on the Vanadis Award presentation at the 9th International Vanadium Symposium. PMID: 25843361 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - March 24, 2015 Category: Biochemistry Authors: Costa Pessoa J Tags: J Inorg Biochem Source Type: research

A novel resting form of the trinuclear copper center in the double mutant of a multicopper oxidase, CueO, Cys500Ser/Glu506Ala.
Abstract A multicopper oxidase, CueO was doubly mutated at its type I copper ligand, Cys500 and an acidic amino acid residue located in the proton transfer pathway, Glu506, to Ser and Ala, respectively. Cys500Ser/Glu506Ala was mainly in a novel resting form to afford the absorption band at ca. 400nm and an EPR signal with a highly anisotropic character derived from type III copper. However, Cys500Ser/Glu506Ala gave the same reaction intermediate (peroxide intermediate) as that from Cys500Ser and Cys500Ser/Glu506Gln. PMID: 25840508 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - March 24, 2015 Category: Biochemistry Authors: Kajikawa T, Sugiyama R, Kataoka K, Sakurai T Tags: J Inorg Biochem Source Type: research

Investigation of VO-salophen complexes electronic structure.
Abstract Vanadyl N,N'-bis(salicylidene)-o-phenylenediamine (salophen) complexes have been extensively investigated by cyclic voltammetry, UV-visible spectroscopy and theoretical calculations in MeCN, THF (tetrahydrofuran) and DMF (N,N-dimethylformamide), in order to elucidate the overall factors that influence the electronic density of the metal and therefore the properties of these complexes in various applications. Different substituents were introduced into the salophen skeleton to change the vanadium electron density. Results obtained and here presented showed that the substituents influence the metal electron...
Source: Journal of Inorganic Biochemistry - March 13, 2015 Category: Biochemistry Authors: Bertini S, Coletti A, Floris B, Conte V, Galloni P Tags: J Inorg Biochem Source Type: research

Synthesis, characterization, hydrolase and catecholase activity of a dinuclear iron(III) complex: Catalytic promiscuity.
Abstract Herein, we report the synthesis and characterization of the new di-iron(III) complex [(bbpmp)(H2O)(Cl)Fe(III)(μ-Ophenoxo)Fe(III)(H2O)Cl)]Cl (1), with the symmetrical ligand 2,6-bis{[(2-hydroxybenzyl)(pyridin-2-yl)methylamino]methyl}-4-methylphenol (H3bbpmp). Complexes 2 with the unsymmetrical ligand H2bpbpmp - {2-[[(2-hydroxybenzyl)(2-pyridylmethyl)]aminomethyl]-6-bis(pyridylmethyl) aminomethyl}-4-methylphenol and 3 with the ligand L(1)=4,11-dimethyl-1,8-bis{2-[N-(di-2-pyridylmethyl)amino]ethyl}cyclam were included for comparison purposes. Complex 1 was characterized through elemental analysis, X-ray c...
Source: Journal of Inorganic Biochemistry - March 5, 2015 Category: Biochemistry Authors: Camargo TP, Maia FF, Chaves C, de Souza B, Bortoluzzi AJ, Castilho N, Bortolotto T, Terenzi H, Castellano EE, Haase W, Tomkowicz Z, Peralta RA, Neves A Tags: J Inorg Biochem Source Type: research

Association of structural modifications with bioactivity in three new copper(II) complexes of Schiff base ligands derived from 5-chlorosalicylaldehyde and amino acids.
Abstract Three novel structurally associated copper(II) complexes [Cu(II)(SalCl-Gly)(H2O)2] (1), [Cu(II)(SalCl-Ala)(H2O)] (2) and [Cu(II)(SalCl-Gly)(bipy)]·0.5H2O (3) (SalCl-Gly=5-chloro-2-hydroxybenzylidene-glycine, SalCl-Ala=5-chloro-2-hydroxybenzylidene-alanine, bipy=2,2'-bipyridine) have been synthesized and characterized by X-ray crystallography, elemental analysis, IR and fluorescence spectroscopy. Single-crystal diffraction reveals that complex 1 is an infinite 1D zigzag chain in which SalCl-Gly serves as both a chelating and a bridging ligand, while complexes 2 and 3 are mononuclear. Cu(II) ions in ...
Source: Journal of Inorganic Biochemistry - March 2, 2015 Category: Biochemistry Authors: Li A, Liu YH, Yuan LZ, Ma ZY, Zhao CL, Xie CZ, Bao WG, Xu JY Tags: J Inorg Biochem Source Type: research

Hypobromous acid, a powerful endogenous electrophile: Experimental and theoretical studies.
In conclusion, the high electrophilicity of HOBr could be behind its well-established deleterious effects. We propose that HOBr is the most powerful endogenous electrophile. PMID: 25771434 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - March 2, 2015 Category: Biochemistry Authors: Ximenes VF, Morgon NH, de Souza AR Tags: J Inorg Biochem Source Type: research

A new mononuclear manganese(III) complex of an unsymmetrical hexadentate N3O3 ligand exhibiting superoxide dismutase and catalase-like activity: synthesis, characterization, properties and kinetics studies.
gnorella SR Abstract A mononuclear Mn(III) complex MnL·4H2O (H3L=1-[N-(2-pyridylmethyl),N-(2-hydroxybenzyl)amino]-3-[N'-(2-hydroxybenzyl),N'-(4-methylbenzyl)amino]propan-2-ol) has been prepared and characterized. This complex catalyzes the dismutation of superoxide efficiently, with catalytic rate constant kcat=1.7×10(6)M(-1)s(-1) and IC50 1.26μM, obtained through the nitro blue tetrazolium photoreduction inhibition superoxide dismutase assay, in aqueous solution of pH7.8. MnL is also able to disproportionate more than 300 equivalents of H2O2 in CH3CN, with initial rate of H2O2 decomposition given ...
Source: Journal of Inorganic Biochemistry - March 2, 2015 Category: Biochemistry Authors: Ledesma GN, Eury H, Anxolabéhère-Mallart E, Hureau C, Signorella SR Tags: J Inorg Biochem Source Type: research

Quantification of bindings of organometallic ruthenium complexes to GSTπ by mass spectrometry.
Quantification of bindings of organometallic ruthenium complexes to GSTπ by mass spectrometry. J Inorg Biochem. 2015 Mar 2;146:44-51 Authors: Lin Y, Huang Y, Zheng W, Wu K, Luo Q, Zhao Y, Xiong S, Wang F Abstract Electrospray ionization mass spectrometry (ESI-MS) has been widely used to identify binding sites of metal complexes to proteins. However, the MS quantification of the metal-protein coordination remains a challenge. We have recently demonstrated by ESI-MS analysis that organometallic ruthenium complexes [(η(6)-arene)Ru(en)Cl](+) (arene=p-cymene (1), biphenyl (2) or 9,10-dihydrophenanth...
Source: Journal of Inorganic Biochemistry - March 2, 2015 Category: Biochemistry Authors: Lin Y, Huang Y, Zheng W, Wu K, Luo Q, Zhao Y, Xiong S, Wang F Tags: J Inorg Biochem Source Type: research

Sunflower metallothionein family characterisation. Study of the Zn(II)- and Cd(II)-binding abilities of the HaMT1 and HaMT2 isoforms.
Abstract Plant metallothioneins (MTs) constitute a family of small Cys-rich proteins capable of coordinating metal ions, significantly differing from microbial and animal MTs. They are divided into four subfamilies depending on the Cys pattern in their sequence. In this work, the MT system of the sunflower plant (Helianthus annuus) has been defined, with ten genes coding for MTs (HaMT) belonging to the four plant MT subfamilies; three HaMT1, four HaMT2, one HaMT3 and two HaMT4 isoforms. The gene expression pattern and capacity to confer metal resistance to yeast cells have been analysed for at least one member of ...
Source: Journal of Inorganic Biochemistry - February 28, 2015 Category: Biochemistry Authors: Tomas M, Pagani MA, Andreo CS, Capdevila M, Atrian S, Bofill R Tags: J Inorg Biochem Source Type: research

Platinated oligomers of bovine pancreatic ribonuclease: Structure and stability.
Abstract The reaction between cis-diamminedichloroplatinum(II) (CDDP), cisplatin, a common anticancer drug, and bovine pancreatic ribonuclease (RNase A), induces extensive protein aggregation, leading to the formation of one dimer, one trimer and higher oligomers whose yields depend on cisplatin/protein ratio. Structural and functional properties of the purified platinated species, together with their spontaneous dissociation and thermally induced denaturation, have been characterized. Platinated species preserve a significant, although reduced, ribonuclease activity. The high resistance of the dimers against diss...
Source: Journal of Inorganic Biochemistry - February 26, 2015 Category: Biochemistry Authors: Picone D, Donnarumma F, Ferraro G, Russo Krauss I, Fagagnini A, Gotte G, Merlino A Tags: J Inorg Biochem Source Type: research

Vanadium(V) complex with Schiff-base ligand containing a flexible amino side chain: Synthesis, structure and reactivity.
s W Abstract The Schiff-base ligand (H2salhyhNH3)Cl (1) derived from salicylaldehyde and 6-aminohexanoic acid hydrazide hydrochloride reacts with ammonium metavanadate in methanol solution to yield the dioxidovanadium(V) complex [VO2(salhyhNH3)] (2). The utilized hydrazone ligand contains a flexible and protonated amino side chain. Crystallization from methanol affords complex 2 in the monoclinic space group P21/n, whereas crystallization from a methanol/water mixture 1:1 yields crystals, containing a water molecule of crystallization per two formula units (2⋅1/2H2O), in the orthorhombic space group Pbcn. In ...
Source: Journal of Inorganic Biochemistry - February 25, 2015 Category: Biochemistry Authors: Nica S, Rudolph M, Lippold I, Buchholz A, Görls H, Plass W Tags: J Inorg Biochem Source Type: research

Europium-doped Gd2O3 nanotubes cause the necrosis of primary mouse bone marrow stromal cells through lysosome and mitochondrion damage.
In this study, the Gd2O3:Eu(3+) nanotubes were prepared and characterized by powder X-ray diffraction (XRD), photoluminescence (PL) excitation and emission spectra, scanning electron microscope (SEM), and transmission electron microscopy (TEM). The cytotoxicity of Gd2O3:Eu(3+) nanotubes on BMSCs and the associated mechanisms were further studied. The results indicated that they could be uptaken into BMSCs by an energy-dependent and macropinocytosis-mediated endocytosis process, and primarily localized in lysosome. Gd2O3:Eu(3+) nanotubes effectively inhibited the viability of BMSCs in concentration and time-dependent manner...
Source: Journal of Inorganic Biochemistry - February 19, 2015 Category: Biochemistry Authors: Jin Y, Chen S, Duan J, Jia G, Zhang J Tags: J Inorg Biochem Source Type: research

A ruthenium(II) complex inhibits tumor growth in vivo with fewer side-effects compared with cisplatin.
Abstract The antitumor activity of a ruthenium(II) polypyridyl complex, Δ-[Ru(bpy)2(HPIP)](ClO4)2 (Δ-Ru1, where bpy=2,2'-bipyridine, HPIP=2-(2-hydroxyphenyl)imidazo[4,5-f][1,10]phenanthroline), was evaluated. The in vivo experiments showed that Δ-Ru1 inhibited the growth of a human cervical carcinoma cell line (HeLa) xenotransplanted into nude mice with efficiency similar to that of cisplatin. Histopathology examination of the tumors from treated xenograft models was consistent with apoptosis in tumor cells. Importantly, in striking contrast with cisplatin, Δ-Ru1 did not cause any detectabl...
Source: Journal of Inorganic Biochemistry - February 14, 2015 Category: Biochemistry Authors: Wang JQ, Zhang PY, Ji LN, Chao H Tags: J Inorg Biochem Source Type: research

Design, synthesis and anticancer activity of diam(m)ine platinum(II) complexes bearing a small-molecular cell apoptosis inducer dichloroacetate.
Abstract Four new diam(m)ine platinum complexes containing the dichloroacetate moiety in 3-dichoroacetoxylcyclobutane-1,1-dicarboxylate as the leaving group were synthesized, characterized by elemental analysis as well as by ESI(+)-MS (electrospray ionization mass spectrometry in positive mode), FT-IR, (1)H- and (13)C-NMR, and evaluated for their in vitro anticancer activity against human lung cancer cell line (A549) and ovarian cancer cell lines (SK-OV-3, SK-OV-3/DDP). Diam(m)ines used in the present study belong to the carriers of six clinically approved platinum drugs. Among the complexes synthesized, complex 2...
Source: Journal of Inorganic Biochemistry - February 12, 2015 Category: Biochemistry Authors: Liu W, Jiang J, Xu Y, Hou S, Sun L, Ye Q, Lou L Tags: J Inorg Biochem Source Type: research

Macrophage and colon tumor cells as targets for a binuclear silver(I) N-heterocyclic carbene complex, an anti-inflammatory and apoptosis mediator.
Abstract Chronic inflammation intensifies the risk for malignant neoplasm, indicating that curbing inflammation could be a valid strategy to prevent or cure cancer. Cancer and inflammation are inter-related diseases and many anti-inflammatory agents are also used in chemotherapy. Earlier, we have reported a series of novel ligands and respective binuclear Ag(I)-NHC complexes (NHC=N-heterocyclic carbene) with potential anticancer activity. In the present study, a newly synthesized salt (II) and respective Ag(I)-NHC complex (III) of comparable molecular framework were prepared for a further detailed study. Prelimina...
Source: Journal of Inorganic Biochemistry - February 7, 2015 Category: Biochemistry Authors: Iqbal MA, Umar MI, Haque RA, Khadeer Ahamed MB, Asmawi MZ, Majid AM Tags: J Inorg Biochem Source Type: research

Cellular organization of siderophore biosynthesis in Pseudomonas aeruginosa: Evidence for siderosomes.
Abstract Pyoverdine I (PVDI) and pyochelin (PCH) are the two major siderophores produced by Pseudomonas aeruginosa PAO1 to import iron. The biochemistry of the biosynthesis of these two siderophores has been described in detail in the literature over recent years. PVDI assembly requires the coordinated action of seven cytoplasmic enzymes and is followed by a periplasmic maturation before secretion of the siderophore into the extracellular medium by the efflux system PvdRT-OpmQ. PCH biosynthesis also involves seven cytoplasmic enzymes but no periplasmic maturation. Recent findings indicate that the cytoplasmic enzy...
Source: Journal of Inorganic Biochemistry - February 4, 2015 Category: Biochemistry Authors: Gasser V, Guillon L, Cunrath O, Schalk IJ Tags: J Inorg Biochem Source Type: research

Vanadium-catalyzed chlorination under molecular oxygen.
Abstract A catalytic chlorination of ketones was performed by using a vanadium catalyst in the presence of Bu4NI and AlCl3 under atmospheric molecular oxygen. This catalytic chlorination could be applied to the chlorination of alkenes to give the corresponding vic-dichlorides. AlCl3 was found to serve as both a Lewis acid and a chloride source to induce the facile chlorination. A combination of Bu4NI and AlI3 in the presence of a vanadium catalyst under atmospheric molecular oxygen induced the iodination of ketones. PMID: 25683370 [PubMed - as supplied by publisher] (Source: Journal of Inorganic Biochemistry)
Source: Journal of Inorganic Biochemistry - February 3, 2015 Category: Biochemistry Authors: Moriuchi T, Fukui Y, Kato S, Kajikawa T, Hirao T Tags: J Inorg Biochem Source Type: research

Heteroditopic P,N ligands in gold(I) complexes: Synthesis, structure and cytotoxicity.
Abstract New heteroditopic, bi- and multidentate imino- and aminophosphine ligands were synthesised and complexed to [AuCl(THT)] (THT=tetrahydrothiophene). X-ray crystallography confirmed Schiff base formation in three products, the successful reduction of the imino-group to the sp(3)-hybridised amine in several instances, and confirmed the formation of mono-gold(I) imino- and aminophosphine complexes for four Au-complexes. Cytotoxicity studies in cancerous and non-cancerous cell lines showed a marked increase in cytotoxicity upon ligand complexation to gold(I). These findings were supported by results from the 60...
Source: Journal of Inorganic Biochemistry - January 30, 2015 Category: Biochemistry Authors: Traut-Johnstone T, Kanyanda S, Kriel FH, Viljoen T, Kotze PD, van Zyl WE, Coates J, Rees DJ, Meyer M, Hewer R, Williams DB Tags: J Inorg Biochem Source Type: research

DNA damage and induction of apoptosis in pancreatic cancer cells by a new dinuclear bis(triazacyclonane) copper complex.
Abstract The dinuclear copper(II) complex [Cu2{bcmp(-H)}(μ-OH)](NO3)2·H2O (1, bcmp=2,6-bis(1,4,7-triazacyclonon-1-ylmethyl)-4-methylphenol) has been synthesized and characterized by electrospray ionization mass spectrometry, potentiometric titration and cyclovoltammetry. The X-ray structure of the analogous perchlorate salt [Cu2{bcmp(-H)}(μ-OH)](ClO4)2·2.5H2O (2) was determined. Cytotoxicity studies showed very promising activity of 1 against various pancreatic tumor cell lines with IC50 values comparable or even lower than those of cisplatin. The Cu complex displayed low toxicity against a hum...
Source: Journal of Inorganic Biochemistry - January 26, 2015 Category: Biochemistry Authors: Montagner D, Gandin V, Marzano C, Erxleben A Tags: J Inorg Biochem Source Type: research

Donor atom electrochemical contribution to redox potentials of square pyramidal vanadyl complexes.
Abstract A simple donor atom additivity relationship has been used to calculate the donor atom electrochemical contribution (DEC) of the Oac (acetylacetonate-enolic oxygen), OPh (phenolic oxygen), SPh (mercaptophenol sulfur), Nam (deprotonate amide nitrogen), Nim (imine nitrogen) and Npy (pyridine nitrogen) to the redox processes of the square pyramidal vanadyl complexes. The study focuses on the amidate vanadyl complexes because of (a) their biological interest and (b) the existence of data from plethora complexes studied in great details. The electrochemical contributions for the vanadyl oxidation and reduction ...
Source: Journal of Inorganic Biochemistry - January 26, 2015 Category: Biochemistry Authors: Vlasiou M, Drouza C, Kabanos TA, Keramidas AD Tags: J Inorg Biochem Source Type: research

Synthesis, structure and biological activity of nickel(II) complexes with mefenamato and nitrogen-donor ligands.
Abstract Six novel nickel(II) complexes with the non-steroidal anti-inflammatory drug mefenamic acid (Hmef) with the nitrogen-donor heterocyclic ligands 2,2'-bipyridine (bipy), 2,2'-bipyridylamine (bipyam), 1,10-phenanthroline (phen), 2,2'-dipyridylketone oxime (Hpko) or pyridine (py) and/or the oxygen-donor ligands CH3OH or H2O were synthesized and characterized by physicochemical and spectroscopic techniques. The crystal structures of [Ni(mef-O)2(bipy)(CH3OH)2] (1), [Ni(mef-O)2(phen)(CH3OH)2] (2), [Ni(mef-O,O')2(bipyam)] (3) and [Ni(mef-O)2(Hpko)2]∙CH3OH (4·CH3OH) were determined by X-ray crystallograph...
Source: Journal of Inorganic Biochemistry - January 26, 2015 Category: Biochemistry Authors: Totta X, Papadopoulou AA, Hatzidimitriou AG, Papadopoulos A, Psomas G Tags: J Inorg Biochem Source Type: research

Synthesis, structural studies and biological activity of new Cu(II) complexes with acetyl derivatives of 7-hydroxy-4-methylcoumarin.
Abstract The new Cu(II) complexes with 6-acetyl-7-hydroxy-4-methylcoumarin (HL1) and 8-acetyl-7-hydroxy-4-methylcoumarin (HL2) have been obtained by the electrochemical method. The density functional theory calculations and X-ray absorption spectroscopy techniques have been used to geometrically describe a series of new compounds. The studies have been focused on the coordination mode of the hydroxy ligands to the metallic centre. The complexes, Cu(HL1)2 and Cu(HL2)2⋅0.5H2O, have flat square geometry with oxygen atoms in the first coordination sphere. Two bidentate anionic coumarins are bonded to the metal ca...
Source: Journal of Inorganic Biochemistry - January 23, 2015 Category: Biochemistry Authors: Klepka MT, Drzewiecka-Antonik A, Wolska A, Rejmak P, Ostrowska K, Hejchman E, Kruszewska H, Czajkowska A, Młynarczuk-Biały I, Ferenc W Tags: J Inorg Biochem Source Type: research