A novel binding-induced DNAzyme motor triggered by survivin mRNA
Anal Bioanal Chem. 2022 Jun 29. doi: 10.1007/s00216-022-04183-4. Online ahead of print.ABSTRACTThe accurate and sensitive detection of survivin mRNA is of great significance for cancer diagnosis and treatment. However, limited by the low-abundance mRNA in live cells, most strategies of survivin mRNA detection that were one-to-one signal-triggered model (one target triggered one signal) were inapplicable in practice. Here, we reported a binding-induced DNAzyme motor triggered by the survivin mRNA, which was a one-to-more signal-triggered model (one target triggered more signals), amplifying the detection signal and enhancin...
Source: Analytical and Bioanalytical Chemistry - June 29, 2022 Category: Chemistry Authors: Chang Liu Jiyu Deng Juan Yi Ru Zhang Lixin Chen Xin Fu Shuzhen Liao Wenjun Yi Guoqiang Zou Hai Yang Source Type: research

Surface-enhanced Raman spectroscopy for drug discovery: peptide-RNA binding
In this study, we extended the approach to probe the binding of potential drugs to RNA targets. To demonstrate the proof of concept, two 16-amino acid residue peptides with close primary structures and different binding affinities to the RNA CUG repeat related to myotonic dystrophy were tested. Three-microliter solutions of the RNA repeat with these peptides at nanomolar concentrations were probed using the developed approach, and the binding of only one peptide was demonstrated. The SER spectra exhibited significant fluctuations along with a sudden strong enhancement as spectra were collected consecutively from individual...
Source: Analytical and Bioanalytical Chemistry - June 28, 2022 Category: Chemistry Authors: Lamyaa M Almehmadi Vibhav A Valsangkar Ken Halvorsen Qiang Zhang Jia Sheng Igor K Lednev Source Type: research

Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus
In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of SeNPs and volatile methylated selenium compounds. SEM-EDS confirmed pure selenium NPs with an average diameter of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium revealed concentration-dependent mechanism...
Source: Analytical and Bioanalytical Chemistry - June 27, 2022 Category: Chemistry Authors: Milan Žižić Marina Stani ć Giuliana Aquilanti Danica Bajuk-Bogdanovi ć Goran Brankovi ć Ivanka Rodi ć Miroslav Živić Joanna Zakrzewska Source Type: research

Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus
In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of SeNPs and volatile methylated selenium compounds. SEM-EDS confirmed pure selenium NPs with an average diameter of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium revealed concentration-dependent mechanism...
Source: Analytical and Bioanalytical Chemistry - June 27, 2022 Category: Chemistry Authors: Milan Žižić Marina Stani ć Giuliana Aquilanti Danica Bajuk-Bogdanovi ć Goran Brankovi ć Ivanka Rodi ć Miroslav Živić Joanna Zakrzewska Source Type: research

Validated single urinary assay designed for exposomic multi-class biomarkers of common environmental exposures
Anal Bioanal Chem. 2022 Jun 27. doi: 10.1007/s00216-022-04159-4. Online ahead of print.ABSTRACTEpidemiological studies often call for analytical methods that use a small biospecimen volume to quantify trace level exposures to environmental chemical mixtures. Currently, as many as 150 polar metabolites of environmental chemicals have been found in urine. Therefore, we developed a multi-class method for quantitation of biomarkers in urine. A single sample preparation followed by three LC injections was optimized in a proof-of-approach for a multi-class method. The assay was validated to quantify 50 biomarkers of exposure in ...
Source: Analytical and Bioanalytical Chemistry - June 26, 2022 Category: Chemistry Authors: Ravikumar Jagani Divya Pulivarthi Dhavalkumar Patel Rosalind J Wright Robert O Wright Manish Arora Mary S Wolff Syam S Andra Source Type: research

Reiner Salzer, Duncan Thorburn Burns, Lothar Beyer: Chemistry as a university degree - development and impact
Anal Bioanal Chem. 2022 Jun 24. doi: 10.1007/s00216-022-04171-8. Online ahead of print.NO ABSTRACTPMID:35739395 | DOI:10.1007/s00216-022-04171-8 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - June 23, 2022 Category: Chemistry Authors: Peeter Burk Source Type: research

Reiner Salzer, Duncan Thorburn Burns, Lothar Beyer: Chemistry as a university degree - development and impact
Anal Bioanal Chem. 2022 Jun 24. doi: 10.1007/s00216-022-04171-8. Online ahead of print.NO ABSTRACTPMID:35739395 | DOI:10.1007/s00216-022-04171-8 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - June 23, 2022 Category: Chemistry Authors: Peeter Burk Source Type: research

Reiner Salzer, Duncan Thorburn Burns, Lothar Beyer: Chemistry as a university degree - development and impact
Anal Bioanal Chem. 2022 Jun 24. doi: 10.1007/s00216-022-04171-8. Online ahead of print.NO ABSTRACTPMID:35739395 | DOI:10.1007/s00216-022-04171-8 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - June 23, 2022 Category: Chemistry Authors: Peeter Burk Source Type: research

Quantitative determination of sn-positional phospholipid isomers in MS < sup > n < /sup > using silver cationization
Anal Bioanal Chem. 2022 Jun 22. doi: 10.1007/s00216-022-04173-6. Online ahead of print.ABSTRACTGlycerophospholipids are one of the fundamental building blocks for life. The acyl chain connectivity to the glycerol backbone constitutes different sn-positional isomers, which have great diversity and importance for biological function. However, to fully realize their impact on function, analytical techniques that can identify and quantify sn-positional isomers in chemically complex biological samples are needed. Here, we utilize silver ion cationization in combination with tandem mass spectrometry (MSn) to identify sn-position...
Source: Analytical and Bioanalytical Chemistry - June 22, 2022 Category: Chemistry Authors: Johan Lillja Ingela Lanekoff Source Type: research

A home-made sampling system coupled to hectowatt-MPT mass spectrometry in positive ion mode to confirm target ions of copper and zinc from Poyang Lake, China
Anal Bioanal Chem. 2022 Jun 22. doi: 10.1007/s00216-022-04172-7. Online ahead of print.ABSTRACTA novel home-made H2SO4-Nafion (HN) tube sampling system coupled to a line ion trap mass spectrometer (LTQ-MS) with a versatile ambient ionization source, hectowatt microwave plasma torch (HMPT), has manifested unique advantages for picking directly metal elements in aqueous samples and acquiring the fully characteristic MPT mass spectra of copper and zinc composite ions. Here, we report the development of a novel HN-HMPT-LTQ-MS for metal elements assay based on environmental water to analyze samples of Poyang Lake, China. Detail...
Source: Analytical and Bioanalytical Chemistry - June 22, 2022 Category: Chemistry Authors: Tao Jiang Feng Jiang Yuhua Zhong Lehui Zhu Zhengxu Huang Zhen Zhou Jianfeng Zou Jianguo Zeng Zhiqiang Zhu Zihui Yang Source Type: research

Long-term continuous monitoring of microRNA in living cells using modified gold nanoprobe
Anal Bioanal Chem. 2022 Jun 22. doi: 10.1007/s00216-022-04182-5. Online ahead of print.ABSTRACTLong-term and continuous monitoring of the microRNA (miRNA) expression in living cells is essential in biomedical research, but it is currently limited by fast consumption and easy digestion of probes in the intracellular environment. Herein, we report polydopamine-modified gold nanoparticles (AuNPs@PDA) as protective and efficient nanocarriers for DNA hairpin probes (hpDNA), achieving long-term monitoring (48 h) of the miRNA (let-7a) levels in living cells after drug treatments. This method enabled excellent sensitivity and high...
Source: Analytical and Bioanalytical Chemistry - June 22, 2022 Category: Chemistry Authors: Yuling Liang Huihui Yang Wen Yin Yanfei Zhang Yuzhi Xu Si-Yang Liu Zong Dai Xiaoyong Zou Source Type: research

Affinity-based isolation of extracellular vesicles and the effects on downstream molecular analysis
Anal Bioanal Chem. 2022 Jun 23. doi: 10.1007/s00216-022-04178-1. Online ahead of print.ABSTRACTExtracellular vesicles (EVs) are transport vesicles with diameters ranging from 30 to 1000 nm, secreted by cells in both physiological and pathological conditions. By using the EV shuttling system, biomolecular cargo such as proteins and genetic materials travels between cells resulting in intercellular communication and epigenetic regulation. Because the presence of EVs and cargo molecules in body fluids can predict the state of the parental cells, EV isolation techniques from complex biofluids have been developed. Further explo...
Source: Analytical and Bioanalytical Chemistry - June 22, 2022 Category: Chemistry Authors: Gisela Str öhle Jingxuan Gan Huiyan Li Source Type: research

Quantitative determination of sn-positional phospholipid isomers in MS < sup > n < /sup > using silver cationization
Anal Bioanal Chem. 2022 Jun 22. doi: 10.1007/s00216-022-04173-6. Online ahead of print.ABSTRACTGlycerophospholipids are one of the fundamental building blocks for life. The acyl chain connectivity to the glycerol backbone constitutes different sn-positional isomers, which have great diversity and importance for biological function. However, to fully realize their impact on function, analytical techniques that can identify and quantify sn-positional isomers in chemically complex biological samples are needed. Here, we utilize silver ion cationization in combination with tandem mass spectrometry (MSn) to identify sn-position...
Source: Analytical and Bioanalytical Chemistry - June 22, 2022 Category: Chemistry Authors: Johan Lillja Ingela Lanekoff Source Type: research

A home-made sampling system coupled to hectowatt-MPT mass spectrometry in positive ion mode to confirm target ions of copper and zinc from Poyang Lake, China
Anal Bioanal Chem. 2022 Jun 22. doi: 10.1007/s00216-022-04172-7. Online ahead of print.ABSTRACTA novel home-made H2SO4-Nafion (HN) tube sampling system coupled to a line ion trap mass spectrometer (LTQ-MS) with a versatile ambient ionization source, hectowatt microwave plasma torch (HMPT), has manifested unique advantages for picking directly metal elements in aqueous samples and acquiring the fully characteristic MPT mass spectra of copper and zinc composite ions. Here, we report the development of a novel HN-HMPT-LTQ-MS for metal elements assay based on environmental water to analyze samples of Poyang Lake, China. Detail...
Source: Analytical and Bioanalytical Chemistry - June 22, 2022 Category: Chemistry Authors: Tao Jiang Feng Jiang Yuhua Zhong Lehui Zhu Zhengxu Huang Zhen Zhou Jianfeng Zou Jianguo Zeng Zhiqiang Zhu Zihui Yang Source Type: research

Long-term continuous monitoring of microRNA in living cells using modified gold nanoprobe
Anal Bioanal Chem. 2022 Jun 22. doi: 10.1007/s00216-022-04182-5. Online ahead of print.ABSTRACTLong-term and continuous monitoring of the microRNA (miRNA) expression in living cells is essential in biomedical research, but it is currently limited by fast consumption and easy digestion of probes in the intracellular environment. Herein, we report polydopamine-modified gold nanoparticles (AuNPs@PDA) as protective and efficient nanocarriers for DNA hairpin probes (hpDNA), achieving long-term monitoring (48 h) of the miRNA (let-7a) levels in living cells after drug treatments. This method enabled excellent sensitivity and high...
Source: Analytical and Bioanalytical Chemistry - June 22, 2022 Category: Chemistry Authors: Yuling Liang Huihui Yang Wen Yin Yanfei Zhang Yuzhi Xu Si-Yang Liu Zong Dai Xiaoyong Zou Source Type: research