Correction to: Adduct annotation in liquid chromatography/high-resolution mass spectrometry to enhance compound identification.
PMID: 33438044 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - January 12, 2021 Category: Chemistry Authors: Stricker T, Bonner R, Lisacek F, Hopfgartner G Tags: Anal Bioanal Chem Source Type: research

Implementing a flipped classroom approach in remote instruction.
PMID: 33426596 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - January 11, 2021 Category: Chemistry Authors: Heiss EM, Oxley SP Tags: Anal Bioanal Chem Source Type: research

Hands-on experiences for remotely taught analytical chemistry laboratories.
PMID: 33423083 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - January 9, 2021 Category: Chemistry Authors: Destino JF, Gross EM, Niemeyer ED, Petrovic SC Tags: Anal Bioanal Chem Source Type: research

A novel concentration gradient microfluidic chip for high-throughput antibiotic susceptibility testing of bacteria.
Abstract Antibiotic resistance has become a serious threat to food safety and public health globally. Therefore, the development of a sensitive, quick, and simple method for antibiotic susceptibility testing is an urgent and crucial need. A novel concentration gradient microfluidic chip was designed in this work to generate antibiotic concentration gradient, culture bacteria, and produce fluorescence emission. An in-house-assembled fluorescence detection platform was constructed, and experiments were conducted to verify the linearity of the generated concentration gradient, explore the appropriate incubation time ...
Source: Analytical and Bioanalytical Chemistry - January 8, 2021 Category: Chemistry Authors: Sun J, Ren Y, Ji J, Guo Y, Sun X Tags: Anal Bioanal Chem Source Type: research

Thermal stability of cannabinoids in dried cannabis: a kinetic study.
This study was undertaken to quantitatively explore the effect of temperature on the degradation of cannabinoids in dried cannabis flower. A total of 14 cannabinoids were monitored using liquid chromatography and tandem mass spectrometry in temperature environments from - 20 to + 40 °C lasting up to 1 year. We find that a network of first-order degradation reactions is well-suited to model the observed changes for all cannabinoids. While most studies focus on high-temperature effects on the cannabinoids, this study provides high-precision quantitative assessment of room temperature kinetics with applicati...
Source: Analytical and Bioanalytical Chemistry - January 8, 2021 Category: Chemistry Authors: Meija J, McRae G, Miles CO, Melanson JE Tags: Anal Bioanal Chem Source Type: research

The potential of fluorescent dyes-comparative study of Nile red and three derivatives for the detection of microplastics.
Abstract During the last years, microplastics in the environment came to the fore in environmental science research. For an appropriate risk assessment, it is essential to know the levels of microplastic contamination in the environment. In the field of microplastic detection, extensive research has been carried out in recent years. While common methods such as Raman spectroscopy and pyrolysis GC-MS are time-consuming and require trained staff and expensive equipment, there is the need for a cheap and easily applicable method. Staining microplastics with the fluorescent dye Nile red (NR) has a high potential to fu...
Source: Analytical and Bioanalytical Chemistry - January 7, 2021 Category: Chemistry Authors: Sturm MT, Horn H, Schuhen K Tags: Anal Bioanal Chem Source Type: research

Metal composite oxides Bi2MoO6/IL membrane as matrix for constructing ultrasensitive electrochemical immunosensor.
Abstract In the process of diagnosis and disease monitoring, it is important to quickly and easily detect protein biomarkers. The strategy reported here is an attempt to prepare Bi2MoO6 nanomaterial with new three-dimensional holes morphology surrounded by rod and sheet to construct a simple and sensitive sensing platform, where Bi2MoO6/ionic liquid (IL) composite was modified on the carbon paste electrode (CPE). In order to monitor the assembly process of human IgG immunosensors, a plurality of electrochemical tests such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) was executed. The...
Source: Analytical and Bioanalytical Chemistry - January 7, 2021 Category: Chemistry Authors: Zhang J, Dong S, Guan L, Zhang D, Huang T Tags: Anal Bioanal Chem Source Type: research

Correction to: Determination of fluoroquinolones in food samples by magnetic solid-phase extraction based on a magnetic molecular sieve nanocomposite prior to high-performance liquid chromatography and tandem mass spectrometry.
PMID: 33409673 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - January 7, 2021 Category: Chemistry Authors: Yu H, Jia Y, Wu R, Chen X, Chan TD Tags: Anal Bioanal Chem Source Type: research

Glutathione conjugates of the mercapturic acid pathway and guanine adduct as biomarkers of exposure to CEES, a sulfur mustard analog.
C, Piérard C, Douki T Abstract Sulfur mustard (SM), a chemical warfare agent, is a strong alkylating compound that readily reacts with numerous biomolecules. The goal of the present work was to define and validate new biomarkers of exposure to SM that could be easily accessible in urine or plasma. Because investigations using SM are prohibited by the Organisation for the Prohibition of Chemical Weapons, we worked with 2-chloroethyl ethyl sulfide (CEES), a monofunctional analog of SM. We developed an ultra-high-pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) approach to the conjugate o...
Source: Analytical and Bioanalytical Chemistry - January 7, 2021 Category: Chemistry Authors: Roser M, Béal D, Eldin C, Gudimard L, Caffin F, Gros-Désormeaux F, Léonço D, Fenaille F, Junot C, Piérard C, Douki T Tags: Anal Bioanal Chem Source Type: research

A novel nanostructured poly(thionine)-deep eutectic solvent/CuO nanoparticle film-modified disposable pencil graphite electrode for determination of acetaminophen in the presence of ascorbic acid.
Abstract A new electrochemical sensor based on thionine (TH), an electroactive polymer, and CuO nanoparticle (CuONP)-modified pencil graphite electrode (PGE) has been developed. Poly(thionine) (PTH) was formed on the CuO/PGE surface by electropolymerisation in ethaline deep eutectic solvent (DES) containing acetic acid dopant to form PTHEthaline/CuO/PGE. Cyclic voltammetry, electrochemical impedance spectroscopy, and differential pulse voltammetry were utilized to evaluate the fabrication process, electrochemical properties, and performance parameters of the modified electrodes. The analytical performance of the P...
Source: Analytical and Bioanalytical Chemistry - January 7, 2021 Category: Chemistry Authors: Dalkiran B, Brett CMA Tags: Anal Bioanal Chem Source Type: research

Polyacrylonitrile-based carbon nanofibers as a matrix for laser desorption/ionization time-of-flight mass spectrometric analysis of small molecules under both positive and negative ionization modes.
Abstract Carbon fiber (CNF), prepared by carbonization of electrospun polyacrylonitrile (PAN) fibers, is systematically investigated as a mediator to replace conventional organic matrices for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS). CNF exhibits a high salt tolerance, sensitivity, and resolution for organic matrix-free laser desorption/ionization time-of-flight mass spectrometry (LDI-MS) analysis of various analytes under both positive and negative ionization modes. Especially, saccharides, a neutral molecule having low negative ionization efficiency, are successfull...
Source: Analytical and Bioanalytical Chemistry - January 6, 2021 Category: Chemistry Authors: Chae A, Lee G, Koh DY, Yang CM, Lee S, Kim YK Tags: Anal Bioanal Chem Source Type: research

Research advances in hydrogen-deuterium exchange mass spectrometry for protein epitope mapping.
We describe the epitope mapping of virus assemblies, disease-associated proteins, and polyclonal antibodies as examples of pattern introduction. Finally, we discuss the outlook of hydrogen-deuterium exchange epitope mapping technology. This review will help researchers studying protein epitopes to gain a more comprehensive understanding of this technology. PMID: 33404742 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - January 6, 2021 Category: Chemistry Authors: Sun H, Ma L, Wang L, Xiao P, Li H, Zhou M, Song D Tags: Anal Bioanal Chem Source Type: research

MRSI vs CEST MRI to understand tomato metabolism in ripening fruit: is there a better contrast?
ny JM Abstract Besides structural information, magnetic resonance imaging (MRI) is crucial to reveal the presence and gradients of metabolites in organs constituted of several tissues. In plant science, such knowledge is key to better understand fruit development and metabolism. Routine methods based on fixation for cytological studies or dissection for metabolite measurements induce biases and plant sample destruction. Magnetic resonance spectroscopy imaging (MSRI) leads to one NMR spectrum per pixel while chemical exchange saturation transfer (CEST) MRI allows mapping metabolites having exchangeable protons. As ...
Source: Analytical and Bioanalytical Chemistry - January 6, 2021 Category: Chemistry Authors: Pagés G, Deborde C, Lemaire-Chamley M, Moing A, Bonny JM Tags: Anal Bioanal Chem Source Type: research

Micro-FTIR combined with curve fitting method to study cellulose crystallinity of developing cotton fibers.
This study aimed to use micro-FTIR with transmission mode to investigate cellulose crystallinity of developing cotton fibers. Compared with ATR-FTIR method, we found that micro-FTIR can obtain more information of cellulose inside of the developing cotton fibers, especially in high wavenumber of 2800-3000 cm-1 region. Combined with curve fitting method, a new IR crystallinity index (CI) method named wax crystallinity index (WCI) was introduced to evaluate the cellulose crystallinity in the development of cotton fibers based on the peak and area ratios of 2900 cm-1/2850 cm-1 and 2900 cm-1/2920 cm-1. ...
Source: Analytical and Bioanalytical Chemistry - January 6, 2021 Category: Chemistry Authors: Zhang L, Li X, Zhang S, Gao Q, Lu Q, Peng R, Xu P, Shang H, Yuan Y, Zou H Tags: Anal Bioanal Chem Source Type: research

Mitochondria-targetable ratiometric fluorescence probe for carbon monoxide based on naphthalimide derivatives.
Abstract The design of ratiometric probes for imaging of carbon monoxide (CO) in subcellular organelles is critical to elucidate its biological and pathological functions. In this work, we establish a ratiometric fluorescent probe (Mito-NIB-CO) for imaging of CO in mitochondria. The mitochondria-targeting unit (triphenylphosphonium moiety) and CO-responsive unit (allyl ether moiety) are covalently linking into the single molecule (Mito-NIB-CO) to achieve the multifunctional effect. Upon being treated with CO, Mito-NIB-CO underwent the cleavage of allyl ether element in the presence of PdCl2, resulting in the struc...
Source: Analytical and Bioanalytical Chemistry - January 6, 2021 Category: Chemistry Authors: Du F, Qu Y, Li M, Tan X Tags: Anal Bioanal Chem Source Type: research

Reference method for off-line analysis of nitrogen oxides in cell culture media by an ozone-based chemiluminescence detector.
Abstract Nitric oxide (NO) and its by-products are important biological signals in human physiology and pathology particularly in the vascular and immune systems. Thus, in situ determination of the NO-related molecule (NOx) levels using embedded sensors is of high importance particularly in the context of cellular biocompatibility testing. However, NOx analytical reference method dedicated to the evaluation of biomaterial biocompatibility testing is lacking. Herein, we demonstrate a PAPA-NONOate-based reference method for the calibration of NOx sensors. After, the validation of this reference method and its potent...
Source: Analytical and Bioanalytical Chemistry - January 6, 2021 Category: Chemistry Authors: Chmayssem A, Monsalve-Grijalba K, Alias M, Mourier V, Vignoud S, Scomazzon L, Muller C, Barthes J, Vrana NE, Mailley P Tags: Anal Bioanal Chem Source Type: research

A novel ratiometric fluorescent probe for differential detection of HSO3- and ClO- and application in cell imaging and tumor recognition.
Abstract By connecting 1,8-naphthalimide and indole sulfonate, a ratio fluorescent probe capable of differential detection of hydrogen sulfite and hypochlorite was synthesized for the first time. It was able to achieve the qualitative detection of HSO3- and ClO- with high sensitivity and selectivity, respectively. It provides a multi-purpose probe and is based on different emission channels without mutual interference. The probe has the advantages of larger Stokes shift (ClO-: 115 nm, HSO3-: 88 nm), longer λem (ClO-: 515 nm, HSO3-: 548 nm) and better water solubility (DMF/PBS =&th...
Source: Analytical and Bioanalytical Chemistry - January 6, 2021 Category: Chemistry Authors: Wu L, Qi S, Liu Y, Wang X, Zhu L, Yang Q, Du J, Xu H, Li Y Tags: Anal Bioanal Chem Source Type: research

Dual-mode imaging of copper transporter 1 in HepG2 cells by hyphenating confocal laser scanning microscopy with laser ablation ICPMS.
Abstract Copper transporter 1 (CTR1) is a transport protein involved in copper and cisplatin uptake. The visualization of cellular CTR1 migration and its redistribution is highly important in copper/cisplatin exposure/transport. However, to the best of our knowledge, this is a highly challenging task. Herein, a dual-mode imaging strategy for CTR1 is developed by hyphenating confocal laser scanning microscopy (CLSM) and laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) with a fluorescent/elemental bifunctional tag conjugated with anti-CTR1 antibody. The tag consists of rhodamine B and zirconium...
Source: Analytical and Bioanalytical Chemistry - January 6, 2021 Category: Chemistry Authors: Sun QX, Wei X, Zhang SQ, Chen ML, Yang T, Yu YL, Wang JH Tags: Anal Bioanal Chem Source Type: research

Development and validation of a selective SPR aptasensor for the detection of anticancer drug irinotecan in human plasma samples.
Abstract In this work, a surface plasmon resonance (SPR)-based assay for the quantification of antineoplastic drug irinotecan in human plasma samples has been developed for the first time. The selective binding of irinotecan with an aptamer receptor, operating in human plasma, allowed to set-up a novel analytical methodology to detect the drug in the analytical range of interest by using SPR as detection technique. After hybridizing the aptamer to the sensing platform and optimizing the sample preparation procedure, a quantitative assay was validated according to FDA regulatory guidelines. The analytical working r...
Source: Analytical and Bioanalytical Chemistry - January 6, 2021 Category: Chemistry Authors: Puscasu A, Zanchetta M, Posocco B, Bunka D, Tartaggia S, Toffoli G Tags: Anal Bioanal Chem Source Type: research

FRET-based fluorescent probe for drug assay from amino acid@gold-carbon nanoparticles.
Abstract Biocompatible and luminescent nanostructures synthesized by capping gold-carbon nanoparticles (HOOC-4-C6H4-AuNPs) with amino acids tyrosine, tryptophan, and cysteine were used for the quantitative estimation of ranitidine (RNH), a peptic ulcer and gastroesophageal reflux drug. We applied a fluorescence quenching mechanism to investigate the viability of the energy transfer based on gold-carbon nanosensors. Förster resonance energy transfer (FRET) calculations showed a donor-acceptor distance of 1.69 nm (Tyr@AuNPs), 2.27 nm (Trp@AuNPs), and 2.32 nm (Cys@AuNPs). The constant time-resolve...
Source: Analytical and Bioanalytical Chemistry - January 6, 2021 Category: Chemistry Authors: Hameed MK, Parambath JBM, Kanan SM, Mohamed AA Tags: Anal Bioanal Chem Source Type: research

A resonance Rayleigh scattering and fluorescence quenching dual-channel sensor for sensitive detection of chitosan based on Eosin Y.
Abstract The sensitive chitosan (CTS) detection methods based on the resonance Rayleigh scattering (RRS) quenching method and fluorescence quenching of Eosin Y were put forward. In the HAC-NaAC buffer solution, Eosin Y interacted with Triton X-100 to generate the binary complex which served as the RRS spectral probe. When CTS was interacted with the binary complex, the RRS intensity decreased with the increase of CTS. At the same time, the fluorescence intensity of Eosin Y decreased in the presence of Triton X-100, and the fluorescence intensity of "Eosin Y+Triton X-100" system further decreased when CTS...
Source: Analytical and Bioanalytical Chemistry - January 5, 2021 Category: Chemistry Authors: Zou W, Song M, He J, Qiu P, Sun Z, Su Z, Bai Y Tags: Anal Bioanal Chem Source Type: research

Complementary neuropeptide detection in crustacean brain by mass spectrometry imaging using formalin and alternative aqueous tissue washes.
Abstract Neuropeptides are low abundance signaling molecules that modulate almost every physiological process, and dysregulation of neuropeptides is implicated in disease pathology. Mass spectrometry (MS) imaging is becoming increasingly useful for studying neuropeptides as new sample preparation methods for improving neuropeptide detection are developed. In particular, proper tissue washes prior to MS imaging have shown to be quick and effective strategies for increasing the number of detectable neuropeptides. Treating tissues with solvents could result in either gain or loss of detection of analytes, and charact...
Source: Analytical and Bioanalytical Chemistry - January 5, 2021 Category: Chemistry Authors: Vu NQ, Buchberger AR, Johnson J, Li L Tags: Anal Bioanal Chem Source Type: research

Non-invasive detection of glucose in human urine using a color-generating copper NanoZyme.
In this study, a free-standing copper NanoZyme is used for the colorimetric detection of glucose in human urine. The sensor could operate in a biologically relevant dynamic linear range of 0.5-15 mM, while showing minimal sample matrix effect such that glucose could be detected in urine without significant sample processing or dilution. This ability could be attributed to the Cu NanoZyme that for the first time showed an ability to promote the oxidation of a TMB substrate to its double oxidation diimine product rather than the charge-transfer complex product commonly observed. Additionally, the sensor could operate at...
Source: Analytical and Bioanalytical Chemistry - January 5, 2021 Category: Chemistry Authors: Naveen Prasad S, Weerathunge P, Karim MN, Anderson S, Hashmi S, Mariathomas PD, Bansal V, Ramanathan R Tags: Anal Bioanal Chem Source Type: research

SERS characterization of aggregated and isolated bacteria deposited on silver-based substrates.
Abstract Surface-enhanced Raman scattering (SERS), based on the enhancement of the Raman signal of molecules positioned within a few nanometres from a structured metal surface, is ideally suited to provide bacterial-specific molecular fingerprints which can be used for analytical purposes. However, for some complex structures such as bacteria, the generation of reproducible SERS spectra is still a challenging task. Among the various factors influencing the SERS variability (such as the nature of SERS-active substrate, Raman parameters and bacterial specificity), we demonstrate in this study that the environment of...
Source: Analytical and Bioanalytical Chemistry - January 3, 2021 Category: Chemistry Authors: Andrei CC, Moraillon A, Larquet E, Potara M, Astilean S, Jakab E, Bouckaert J, Rosselle L, Skandrani N, Boukherroub R, Ozanam F, Szunerits S, Gouget-Laemmel AC Tags: Anal Bioanal Chem Source Type: research

High-performance thin-layer chromatography in combination with a yeast-based multi-effect bioassay to determine endocrine effects in environmental samples.
In this study, a yeast multi endocrine-effect screen (YMEES) for the detection of endocrine effects is combined with HPTLC. Simultaneous detection of estrogenic, androgenic, and gestagenic effects on the HPTLC plate is achieved by mixing different genetically modified Arxula adeninivorans yeast strains, which contain either the human estrogen, androgen, or progesterone receptor. Depending on the yeast strain, different fluorescent proteins are formed when an appropriate substance binds to the specific hormone receptor. This allows to measure hormonal effects at different wavelengths. Two yeast cell application approaches, ...
Source: Analytical and Bioanalytical Chemistry - January 3, 2021 Category: Chemistry Authors: Baetz N, Rothe L, Wirzberger V, Sures B, Schmidt TC, Tuerk J Tags: Anal Bioanal Chem Source Type: research

Development of capillary-paper spray for small-molecule analysis in complex samples.
Abstract We develop a capillary-paper spray (CPS) ion source which allows for sample separation in the capillary and enables rapid and sensitive paper spray (PS) mass spectrometry (MS) analysis of biofluids. The CPS employs a glass capillary to load liquid analytes, vertically standing at the rear of the PS. To further reduce the matrix effect, a nitrocellulose filter membrane is placed between the glass tube and chromatography paper to absorb proteins and other macromolecules, which is beneficial for the detection of the small molecules. Compared with the normal PS method, the CPS method markedly improves spray s...
Source: Analytical and Bioanalytical Chemistry - January 3, 2021 Category: Chemistry Authors: Liu C, Li Y, Xiong C, Nie Z Tags: Anal Bioanal Chem Source Type: research

Selective optosensing of iron(III) ions in HeLa cells using NaYF4:Yb3+/Tm3+ upconversion nanoparticles coated with polyepinephrine.
Abstract Novel polyepinephrine-modified NaYF4:Yb,Tm upconversion luminescent nanoparticles (UCNP@PEP) were prepared via the self-polymerization of epinephrine on the surfaces of the UCNPs for selective sensing of Fe3+ inside a cell and for intracellular imaging. The proposed UCNP@PEP probe is a strong blue light emitter (λmax = 474 nm) upon exposure to an excitation wavelength of 980 nm. The probe was used for detecting Fe3+ owing to the complexation reaction between UCNP@PEP and Fe3+, resulting in reduced upconversion luminescence (UCL) intensity. The proposed probe has a detection ...
Source: Analytical and Bioanalytical Chemistry - January 3, 2021 Category: Chemistry Authors: Gerelkhuu Z, Huy BT, Jung D, Sharipov M, Lee YI Tags: Anal Bioanal Chem Source Type: research

Developing an integrated microfluidic and miniaturized electrochemical biosensor for point of care determination of glucose in human plasma samples.
Abstract A cost-effective, point of care (POC) device based on highly oriented CNT arrays was developed as an electrochemical assay for real-time and sensitive detection of glucose in complex samples. A low-cost, microcontroller-based potentiostat consisting of Arduino Due and LMP9100-EVM was developed to perform electrochemical measurements such as cyclic voltammetry (CV) and amperometry. A syringe pump based on open-source electronics was designed to direct the flow through a microfluidic chip. Vertically aligned carbon nanotube (VACNT) sensor arrays, in combination with the miniature potentiostat and the syring...
Source: Analytical and Bioanalytical Chemistry - January 2, 2021 Category: Chemistry Authors: Azimi S, Farahani A, Docoslis A, Vahdatifar S Tags: Anal Bioanal Chem Source Type: research

Evaluation of different approaches to applying the standard additions calibration method.
ti GL Abstract The extrapolation approach, traditionally used with standard additions (SA), is compared with the alternative strategies of interpolation, reversed-axis, and normalization. The interpolation approach is based on employing twice the analytical signal recorded for the sample (ysam) to determine an unknown analyte concentration. In the reversed-axis strategy, x- and y-axes are swapped when building the SA calibration plot to facilitate uncertainty estimation. A new strategy, based on signal normalization using ysam, is also described and compared to the other approaches. Results from 3 instrumental met...
Source: Analytical and Bioanalytical Chemistry - January 2, 2021 Category: Chemistry Authors: Sloop JT, Gonçalves DA, O'Brien LM, Carter JA, Jones BT, Donati GL Tags: Anal Bioanal Chem Source Type: research

Sensitive ratiometric fluorescence assay for detecting xanthine in serum based on the inner filter effect of enzyme-catalyzed oxidation products to silicon nanoparticles.
Abstract A new type of fluorescent silicon nanoparticles (SiNPs) were prepared via a facile one-pot hydrothermal method by using N-[3-(trimethoxysilyl)propyl]-ethylenediamine (DAMO) and glucose as reagents, and were subsequently applied to construct a ratiometric fluorescence assay for sensitive and rapid determination of xanthine in human serum. Two catalytic oxidation reactions were employed to induce a fluorescence response of the testing system towards xanthine. Under the catalysis of xanthine oxidase (XOD), xanthine in serum samples was oxidized and produced hydrogen peroxide (H2O2). By utilizing o-pheny...
Source: Analytical and Bioanalytical Chemistry - January 2, 2021 Category: Chemistry Authors: Li D, Chen F, Li N, Ye X, Sun Y, Ma P, Song D, Wang X Tags: Anal Bioanal Chem Source Type: research

A new method for the preconcentrations of U(VI) and Th(IV) by magnetized thermophilic bacteria as a novel biosorbent.
S, Soylak M Abstract This paper proposes the use of Anoxybacillus flavithermus SO-15 immobilized on iron oxide nanoparticles (NPs) as a novel magnetized biosorbent for the preconcentrations of uranium (U) and thorium (Th). The SPE procedure was based on biosorption of U(VI) and Th(IV) on a column of iron oxide NPs loaded with dead and dried thermophilic bacterial biomass prior to U(VI) and Th(IV) measurements by ICP-OES. The biosorbent characteristicswere explored using FT-IR, SEM, and EDX. Significant operational factors such as solution pH, volume and flow rate of the sample solution, amounts of dead bacter...
Source: Analytical and Bioanalytical Chemistry - January 2, 2021 Category: Chemistry Authors: Kılınç E, Ozdemir S, Yalçın MS, Soylak M Tags: Anal Bioanal Chem Source Type: research

Three-dimensional (3D) imaging of lipids in skin tissues with infrared matrix-assisted laser desorption electrospray ionization (MALDESI) mass spectrometry.
In this study, we describe the use of infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) in ablation-based 3D MSI for analyses of lipids within fresh frozen skin tissue. Depth resolution using different laser energy levels was explored with a confocal laser scanning microscope to establish the imaging parameters for skin. The lowest and highest laser energy level resulted in a depth resolution of 7 μm and 18 μm, respectively. A total of 594 lipids were putatively detected and detailed lipid profiles across different skin layers were revealed in a 56-layer 3D imaging experiment. C...
Source: Analytical and Bioanalytical Chemistry - January 2, 2021 Category: Chemistry Authors: Bai H, Linder KE, Muddiman DC Tags: Anal Bioanal Chem Source Type: research

Metabolomics approach based on LC-HRMS for the fast screening of iron(II)-chelating peptides in protein hydrolysates.
Abstract Production of iron-chelating peptides from protein hydrolysates requires robust and adequate screening methods to optimize their purification and subsequently valorize their potential antioxidant properties. An original methodology was developed for direct and sensitive screening of iron(II)-chelating peptides based on ion-pair reverse phase liquid chromatography (IP-RPLC) coupled to high-resolution mass spectrometry (HRMS). Peptide mixture was first added to iron(II) solution to form iron(II)-peptide complexes. Then IP-RPLC-HRMS analysis was conducted on this iron-peptide mixture and on the iron-free pep...
Source: Analytical and Bioanalytical Chemistry - January 2, 2021 Category: Chemistry Authors: Paris C, Selmeczi K, Ebel B, Stefan L, Csire G, Cakir-Kiefer C, Desobry S, Canabady-Rochelle L, Chaimbault P Tags: Anal Bioanal Chem Source Type: research

Iodine-doped carbon dots with inherent peroxidase catalytic activity for photocatalytic antibacterial and wound disinfection.
Abstract A new type of nitrogen-iodine co-doped carbon dot (N/I-CD) was synthesized by a one-step hydrothermal method with a fluorescence quantum yield of 37%. The prepared N/I-CDs exhibit peroxidase-like activity, can catalyze bio-safety levels of H2O2 to generate hydroxyl radicals (•OH) under visible light and enhance the level of reactive oxygen species (ROS) in bacteria cells. All in vitro experiments showed that the designed system has strong photocatalytic antibacterial activity against both E. coli and S. aureus bacteria. The light-induced antibacterial function of N/I-CDs was evaluated under the condi...
Source: Analytical and Bioanalytical Chemistry - January 2, 2021 Category: Chemistry Authors: Wang X, Lu Y, Hua K, Yang D, Yang Y Tags: Anal Bioanal Chem Source Type: research

Mitochondrion-targeting near-infrared fluorescent probe for detecting intracellular nanomolar level hydrogen sulfide with high recognition rate.
Abstract Hydrogen sulfide (H2S) typically plays biphasic biological roles in living organisms with subnormal H2S exerting cytoprotective effects such as participating in cardioprotective signaling pathways while H2S with higher-than-normal concentrations in localized tissues acting the opposite way such as inhibiting mitochondrial respiration. Such concentration-dependent biological and pathological roles of H2S with the wide involvement of mitochondria and the elusive feature of H2S definitely highlight the vital significance of fast and precise estimation of the physiological level of H2S in specific microenviro...
Source: Analytical and Bioanalytical Chemistry - January 2, 2021 Category: Chemistry Authors: Zhang K, Meng J, Bao W, Liu M, Wang X, Tian Z Tags: Anal Bioanal Chem Source Type: research

Repeated double cross-validation applied to the PCA-LDA classification of SERS spectra: a case study with serum samples from hepatocellular carcinoma patients.
Bonifacio A Abstract Intense label-free surface-enhanced Raman scattering (SERS) spectra of serum samples were rapidly obtained on Ag plasmonic paper substrates upon 785 nm excitation. Spectra from the hepatocellular carcinoma (HCC) patients showed consistent differences with respect to those of the control group. In particular, uric acid was found to be relatively more abundant in patients, while hypoxanthine, ergothioneine, and glutathione were found as relatively more abundant in the control group. A repeated double cross-validation (RDCV) strategy was applied to optimize and validate principal component a...
Source: Analytical and Bioanalytical Chemistry - December 8, 2020 Category: Chemistry Authors: Gurian E, Di Silvestre A, Mitri E, Pascut D, Tiribelli C, Giuffrè M, Crocè LS, Sergo V, Bonifacio A Tags: Anal Bioanal Chem Source Type: research

Recent advances in 2D hexagonal boron nitride (2D-hBN) applied as the basis of electrochemical sensing platforms.
ks CE Abstract 2D hexagonal boron nitride (2D-hBN) is a lesser utilised material than other 2D counterparts in electrochemistry due to initial reports of it being non-conductive. As we will demonstrate in this review, this common misconception is being challenged, and researchers are starting to utilise 2D-hBN in the field of electrochemistry, particularly as the basis of electroanalytical sensing platforms. In this critical review, we overview the use of 2D-hBN as an electroanalytical sensing platform summarising recent developments and trends and highlight future developments of this interesting, often overlooke...
Source: Analytical and Bioanalytical Chemistry - December 7, 2020 Category: Chemistry Authors: García-Miranda Ferrari A, Rowley-Neale SJ, Banks CE Tags: Anal Bioanal Chem Source Type: research

Ex vivo thyroid fine needle aspirations as an alternative for MALDI-MSI proteomic investigation: intra-patient comparison.
In conclusion, these results show that post-surgical FNAs represent a possible alternative source of material for MALDI-MSI proteomic investigations in instances where pre-surgical samples are unavailable or the number of cells is scarce. PMID: 33277997 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - December 5, 2020 Category: Chemistry Authors: Piga I, Capitoli G, Clerici F, Mahajneh A, Brambilla V, Smith A, Leni D, L'Imperio V, Galimberti S, Pagni F, Magni F Tags: Anal Bioanal Chem Source Type: research

Superresolving the kidney-a practical comparison of fluorescence nanoscopy of the glomerular filtration barrier.
nski CF Abstract Immunofluorescence microscopy is routinely used in the diagnosis of and research on renal impairments. However, this highly specific technique is restricted in its maximum resolution to about 250 nm in the lateral and 700 nm in the axial directions and thus not sufficient to investigate the fine subcellular structure of the kidney's glomerular filtration barrier. In contrast, electron microscopy offers high resolution, but this comes at the cost of poor preservation of immunogenic epitopes and antibody penetration alongside a low throughput. Many of these drawbacks were overcome with the...
Source: Analytical and Bioanalytical Chemistry - December 5, 2020 Category: Chemistry Authors: Wunderlich LCS, Ströhl F, Ströhl S, Vanderpoorten O, Mascheroni L, Kaminski CF Tags: Anal Bioanal Chem Source Type: research

Molecular insights into symbiosis-mapping sterols in a marine flatworm-algae-system using high spatial resolution MALDI-2-MS imaging with ion mobility separation.
Abstract Waminoa sp. acoel flatworms hosting Symbiodiniaceae and the related Amphidinium dinoflagellate algae are an interesting model system for symbiosis in marine environments. While the host provides a microhabitat and safety, the algae power the system by photosynthesis and supply the worm with nutrients. Among these nutrients are sterols, including cholesterol and numerous phytosterols. While it is widely accepted that these compounds are produced by the symbiotic dinoflagellates, their transfer to and fate within the sterol-auxotrophic Waminoa worm host as well as their role in its metabolism are unknown. H...
Source: Analytical and Bioanalytical Chemistry - December 3, 2020 Category: Chemistry Authors: Bien T, Hambleton EA, Dreisewerd K, Soltwisch J Tags: Anal Bioanal Chem Source Type: research

Hyphenated high-resolution mass spectrometry-the "all-in-one" device in analytical toxicology?
Hyphenated high-resolution mass spectrometry-the "all-in-one" device in analytical toxicology? Anal Bioanal Chem. 2020 Nov 28;: Authors: Maurer HH Abstract This trend article reviews papers with hyphenated high-resolution mass spectrometry (HRMS) approaches applied in analytical toxicology, particularly in clinical and forensic toxicology published since 2016 and referenced in PubMed. The article focuses on the question of whether HRMS has or will become the all-in-one device in these fields as supposed by the increasing number of HRMS presentations at scientific meetings, corresponding orig...
Source: Analytical and Bioanalytical Chemistry - November 28, 2020 Category: Chemistry Authors: Maurer HH Tags: Anal Bioanal Chem Source Type: research

Highly sensitive electrochemical BPA sensor based on titanium nitride-reduced graphene oxide composite and core-shell molecular imprinting particles.
Abstract A sensitive electrochemical sensor was proposed via combining molecular imprinting technique with the graphene material-doped titanium nitride. The novel graphene with 3-dimensional structure displayed more binding sites and better electrochemical properties. Moreover, this study focused on coating pyrrole with electrical conductivity on the surface of silica as a monomer, and BPA as the template. The interaction made specific detection possible, between monomer and template. With a series of characterizations and electrochemical measurements, CPE (carbon paste electrode)-contained TiN-rGO composite was p...
Source: Analytical and Bioanalytical Chemistry - November 28, 2020 Category: Chemistry Authors: Xu W, Zhang Y, Yin X, Zhang L, Cao Y, Ni X, Huang W Tags: Anal Bioanal Chem Source Type: research

Fluorescence anisotropy study of radiation-induced DNA damage clustering based on FRET.
Abstract A clustered DNA damage site (cluster), in which two or more lesions exist within a few helical turns, is believed to be a key factor determining the fate of a living cell exposed to a DNA damaging agent such as ionizing radiation. However, the structural details of a cluster such as the number of included lesions and their proximity are unknown. Herein, we develop a method to characterize a cluster by fluorescence anisotropy measurements based on Förster resonance energy transfer (homo-FRET). Plasmid DNA (pUC19) was irradiated with 2.0 and 0.52 MeV/u 4He2+, or 0.37 MeV/u 12C5+ ion beams (li...
Source: Analytical and Bioanalytical Chemistry - November 27, 2020 Category: Chemistry Authors: Akamatsu K, Shikazono N, Saito T Tags: Anal Bioanal Chem Source Type: research

Potential impact of tissue molecular heterogeneity on ambient mass spectrometry profiles: a note of caution in choosing the right disease model.
Abstract This review provides a summary of known molecular alterations in commonly used cancer models and strives to stipulate how they may affect ambient mass spectrometry profiles. Immortalized cell lines are known to accumulate mutations, and xenografts derived from cell lines are known to contain tumour microenvironment elements from the host animal. While the use of human specimens for mass spectrometry profiling studies is highly encouraged, patient-derived xenografts with low passage numbers could provide an alternative means of amplifying material for ambient MS research when needed. Similarly, geneti...
Source: Analytical and Bioanalytical Chemistry - November 27, 2020 Category: Chemistry Authors: Katz L, Woolman M, Tata A, Zarrine-Afsar A Tags: Anal Bioanal Chem Source Type: research

Rapid and simple pressure-sensitive adhesive microdevice fabrication for sequence-specific capture and fluorescence detection of sepsis-related bacterial plasmid gene sequences.
Abstract Microbial resistance to currently available antibiotics poses a great threat in the global fight against infections. An important step in determining bacterial antibiotic resistance can be selective DNA sequence capture and fluorescence labeling. In this paper, we demonstrate the fabrication of simple, robust, inexpensive microfluidic devices for DNA capture and fluorescence detection of a model antibiotic resistance gene sequence. We laser micromachined polymethyl methacrylate microchannels and enclosed them using pressure-sensitive adhesive tapes. We then formed porous polymer monoliths with DNA capture...
Source: Analytical and Bioanalytical Chemistry - November 27, 2020 Category: Chemistry Authors: Akuoko Y, Hanson RL, Harris DH, Nielsen JB, Lazalde E, Woolley AT Tags: Anal Bioanal Chem Source Type: research

Microfluidic and mathematical modeling of aquatic microbial communities.
Abstract Aquatic microbial communities contribute fundamentally to biogeochemical transformations in natural ecosystems, and disruption of these communities can lead to ecological disasters such as harmful algal blooms. Microbial communities are highly dynamic, and their composition and function are tightly controlled by the biophysical (e.g., light, fluid flow, and temperature) and biochemical (e.g., chemical gradients and cell concentration) parameters of the surrounding environment. Due to the large number of environmental factors involved, a systematic understanding of the microbial community-environment inter...
Source: Analytical and Bioanalytical Chemistry - November 26, 2020 Category: Chemistry Authors: Liu F, Giometto A, Wu M Tags: Anal Bioanal Chem Source Type: research

Development of an LC-MS method for the semiquantitative determination of polyamide 6 contaminations in polyolefin recyclates.
Abstract Recycling will be of increasing importance in the future, especially for plastic packaging waste mainly consisting of polyolefins. One major problem of recyclates comprises impurities which can have a significant negative impact on future product properties. Polyamide 6 can be found widely as contaminant in recycled polyolefins, leading to a need of quantification methods thereof. In this paper, a method development for the quantitative analysis of polyamide 6 is presented based on analysing ε-caprolactam and related cyclic oligomers as marker compounds in model recyclates of high- and low-density...
Source: Analytical and Bioanalytical Chemistry - November 26, 2020 Category: Chemistry Authors: Schweighuber A, Gall M, Fischer J, Liu Y, Braun H, Buchberger W Tags: Anal Bioanal Chem Source Type: research

Glycine additive facilitates site-specific glycosylation profiling of biopharmaceuticals by ion-pairing hydrophilic interaction chromatography mass spectrometry.
Abstract Many biotherapeutics such as monoclonal antibodies (mAb) and Fc-domain fusion proteins contain heterogeneous glycan contents at one or multiple glycosylation site(s). Site-specific glycan profile characterization is critical for monitoring the quality of these molecules during different stages of drug development. Hydrophilic interaction chromatography (HILIC) as an orthogonal separation method to reversed-phase liquid chromatography (RPLC) can achieve better glycopeptide identification due to the effective separation between individual glycoforms as well as the separation of glycopeptides from high-abund...
Source: Analytical and Bioanalytical Chemistry - November 26, 2020 Category: Chemistry Authors: Zhao Y, Raidas S, Mao Y, Li N Tags: Anal Bioanal Chem Source Type: research

A comparative analysis of different biofluids towards ovarian cancer diagnosis using Raman microspectroscopy.
Abstract Biofluids, such as blood plasma or serum, are currently being evaluated for cancer detection using vibrational spectroscopy. These fluids contain information of key biomolecules, such as proteins, lipids, carbohydrates and nucleic acids, that comprise spectrochemical patterns to differentiate samples. Raman is a water-free and practically non-destructive vibrational spectroscopy technique, capable of recording spectrochemical fingerprints of biofluids with minimum or no sample preparation. Herein, we compare the performance of these two common biofluids (blood plasma and serum) together with ascitic fluid...
Source: Analytical and Bioanalytical Chemistry - November 26, 2020 Category: Chemistry Authors: Giamougiannis P, Morais CLM, Grabowska R, Ashton KM, Wood NJ, Martin-Hirsch PL, Martin FL Tags: Anal Bioanal Chem Source Type: research

Fluorescence polarization immunoassay for the determination of diclofenac in wastewater.
In this study, new tracer molecules with different structures, linking fluorophores to derivatives of the analyte, were synthesized, three homologous tracers based on DCF, two including a C6 spacer, and one heterologous tracer derived from 5-hydroxy-DCF. The tracer molecules were thoroughly assessed for performance. Regarding sensitivity of the FPIA, the lowest limit of detection reached was 2.0 μg L-1 with a working range up to 870 μg L-1. The method was validated for real wastewater samples against LC-MS/MS as reference method with good agreement of both methods. Graphical abstract. PMID: 3...
Source: Analytical and Bioanalytical Chemistry - November 25, 2020 Category: Chemistry Authors: Raysyan A, Moerer R, Coesfeld B, Eremin SA, Schneider RJ Tags: Anal Bioanal Chem Source Type: research