Detection of systemic pesticide residues in tea products at trace level based on SERS and verified by GC-MS.
Abstract Surface-enhanced Raman spectroscopy (SERS) has the potential to detect pesticide residues in agricultural products. However, some systemic pesticides, such as chlorpyrifos, can enter the plant tissue, and not just stay on the surface. Consequently, many SERS studies halted at practical application because of its complexity. In this work, SERS technology was used to detect chlorpyrifos residues in tea products at the semiquantitative level. A simple pretreatment method effectively avoided interference of other fluorescent substances, and all major peaks could be distinguished on the basis of a novel substr...
Source: Analytical and Bioanalytical Chemistry - October 16, 2019 Category: Chemistry Authors: Zhang, Liang P, Ye J, Xia J, Zhou Y, Huang J, Ni D, Tang L, Jin S, Yu Z Tags: Anal Bioanal Chem Source Type: research

Neural network-based fingerprinting of monoclonal antibody aggregation using biolayer interferometry.
In this study, we have developed model based on artificial neural network (ANN) to predict the stress history of mAb contributing to the aggregate formation, based on binding sensogram profiles obtained with biolayer interferometry (BLI). It was observed that each class of mAb aggregates exhibited unique binding profiles that were characteristic fingerprint of that class. The proposed model uses principal components extracted from the mAb-Fcγ receptor binding sensogram (106 profiles from 9 stressed mAb samples) as inputs while the unique identification codes in the form of binary coded numbers are used as model outpu...
Source: Analytical and Bioanalytical Chemistry - October 16, 2019 Category: Chemistry Authors: Budholiya N, Roy S, Rathore AS Tags: Anal Bioanal Chem Source Type: research

Evaluation of different ionic liquid stationary phases for the analysis of carbohydrates by gas chromatography-mass spectrometry.
L, Ruiz-Matute AI Abstract Five commercial ionic liquid (IL) columns have been evaluated for the first time for the gas chromatography-mass spectrometry (GC-MS) analysis of low molecular weight carbohydrate (LMWC) standards (mono-, di-, and trisaccharides, inositols, and iminosugars). A previous derivatization step was necessary to convert the LMWCs into their volatile and stable derivatives. Compared with conventional GC stationary phases, such as HP-1 and Supelcowax® 10, IL columns have shown a different selectivity in the separation of target compounds. Among the IL columns, only SLB™-IL82 allowed the...
Source: Analytical and Bioanalytical Chemistry - October 15, 2019 Category: Chemistry Authors: Rodríguez-Sánchez S, Soria AC, Lebrón-Aguilar R, Sanz ML, Ruiz-Matute AI Tags: Anal Bioanal Chem Source Type: research

Online mass spectrometry of CE (SDS)-separated proteins by two-dimensional capillary electrophoresis.
ml;ß C Abstract Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is the fundamental technique for protein separation by size. Applying this technology in capillary format, gaining high separation efficiency in a more automated way, is a key technology for size separation of proteins in the biopharmaceutical industry. However, unequivocal identification by online mass spectrometry (MS) is impossible so far, due to strong interference in the electrospray process by SDS and other components of the SDS-MW separation gel buffer. Here, a heart-cut two-dimensional electrophoretic separation syst...
Source: Analytical and Bioanalytical Chemistry - October 15, 2019 Category: Chemistry Authors: Römer J, Montealegre C, Schlecht J, Kiessig S, Moritz B, Neusüß C Tags: Anal Bioanal Chem Source Type: research

Speciation analysis of arsenic in samples containing high concentrations of chloride by LC-HG-AFS.
Abstract Chloride widely exists in the environment and will cause serious interference for arsenic speciation analysis. The determination of four arsenic species including arsenite (As(III)), arsenate (As(V)), monomethylarsenate (MMA), and dimethylarsonate (DMA) in samples containing high concentrations of Cl- was carried out in this work by coupling of liquid chromatography (LC) with hydride generation atomic fluorescence spectrometry (HG-AFS). The interference of Cl- was successfully eliminated by coupling two anion-exchange chromatographic columns in series and eluting with 35.0 mmol L-1 (NH4)2HPO4 (p...
Source: Analytical and Bioanalytical Chemistry - October 14, 2019 Category: Chemistry Authors: Yu X, Cui W, Wang Q, Guo Y, Deng T Tags: Anal Bioanal Chem Source Type: research

LC-MS/MS method for the determination of organophosphorus pesticides and their metabolites in salmon and zebrafish fed with plant-based feed ingredients.
MHG, Hernández F Abstract The composition of Atlantic salmon feed has changed considerably over the last two decades from being marine-based (fishmeal and fish oil) to mainly containing plant ingredients. Consequently, concern related to traditional persistent contaminants typically associated with fish-based feed has been replaced by other potential contaminants not previously associated with salmon farming. This is the case for many pesticides, which are used worldwide to increase food production, and may be present in plant ingredients. Earlier studies have identified two organophosphorus pesticides, chl...
Source: Analytical and Bioanalytical Chemistry - October 13, 2019 Category: Chemistry Authors: Garlito B, Ibáñez M, Portolés T, Serrano R, Amlund H, Lundebye AK, Sanden M, Berntssen MHG, Hernández F Tags: Anal Bioanal Chem Source Type: research

Multivariate data analysis of capacitance frequency scanning for online monitoring of viable cell concentrations in small-scale bioreactors.
Abstract Viable cell concentration (VCC) is one of the most important process attributes during mammalian cell cultivations. Current state-of-the-art measurements of VCC comprise offline methods which do not allow for continuous process data. According to the FDA's process analytical technology initiative, process monitoring and control should be applied to gain process understanding and to ensure high product quality. In this work, the use of an inline capacitance probe to monitor online VCCs of a mammalian CHO cell culture process in small-scale bioreactors (250 mL) was investigated. Capacitance sensors usi...
Source: Analytical and Bioanalytical Chemistry - October 13, 2019 Category: Chemistry Authors: Metze S, Blioch S, Matuszczyk J, Greller G, Grimm C, Scholz J, Hoehse M Tags: Anal Bioanal Chem Source Type: research

Gerhard K. E. Scriba (Ed.): Chiral separations: methods and protocols, 3rd ed.
PMID: 31599333 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - October 10, 2019 Category: Chemistry Authors: Wenzel TJ Tags: Anal Bioanal Chem Source Type: research

Visual and spectrophotometric detection of metformin based on the host-guest molecular recognition of cucurbit[6]uril-modified silver nanoparticles.
Abstract A simple, sensitive, and naked-eye assay of metformin (MET), based on the host-guest molecular recognition of cucurbit[6]uril (CB[6])-modified silver nanoparticles, has been developed for the first time. The molecular recognition between CB[6] and MET is initially demonstrated and the related recognition mechanism is further discussed. CB[6]-modified AgNPs were first synthesized and then characterized by UV-vis spectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy. The solution behavior of CB[6] in the presence of AgNO3 was also studied, and the correlative result rev...
Source: Analytical and Bioanalytical Chemistry - October 9, 2019 Category: Chemistry Authors: Song Z, Yu L, Sun Y, He H Tags: Anal Bioanal Chem Source Type: research

Simultaneous determination of proteins in microstructured optical fibers supported by chemometric tools.
Abstract A new perspective on the relevant problem-creating simple, rapid, and efficient protein sensors based on microstructured optical fibers using a simple homogeneous analysis format-was proposed. Commercially available long-period grating hollow core microstructured optical fibers (LPG HCMOF) were used to determine bovine serum albumin (BSA) and albumin from chicken eggs (OVA) in binary mixtures as well as immunoglobulin G (IgG) in the presence of BSA and OVA. LPG HCMOF transmission spectra allowed the detection of both BSA and OVA up to 10 mg/mL with LOD as low as 0.1 and 0.8 μg/mL, respectivel...
Source: Analytical and Bioanalytical Chemistry - October 9, 2019 Category: Chemistry Authors: Burmistrova NA, Pidenko PS, Pidenko SA, Skibina YS, Monakhova YB Tags: Anal Bioanal Chem Source Type: research

Synthesis and characterization of deep eutectic solvents (five hydrophilic and three hydrophobic), and hydrophobic application for microextraction of environmental water samples.
Abstract Hydrophilic and hydrophobic deep eutectic solvents (DESs) as "green" solvents were applied in this study for the microextraction of environmental samples. A series of DESs (five hydrophilic and three hydrophobic) were synthesized and characterized by Fourier transform infrared spectroscopy. Physicochemical property parameters of eight DESs including water solubility, density, conductivity, and freezing point were assessed. Compared with the performance of five hydrophilic DESs in water phase, the three hydrophobic DESs were more suitable for application in dispersive liquid-liquid microextractio...
Source: Analytical and Bioanalytical Chemistry - October 8, 2019 Category: Chemistry Authors: Qu Q, Lv Y, Liu L, Row KH, Zhu T Tags: Anal Bioanal Chem Source Type: research

A simple and efficient method for potential point-of-care diagnosis of human papillomavirus genotypes: combination of isothermal recombinase polymerase amplification with lateral flow dipstick and reverse dot blot.
Abstract Cervical cancer is the second most common cancer in the world's woman population with a high incidence in developing countries where diagnostic conditions for the cancer are poor. The main culprit causing the cancer is the human papillomavirus (HPV). HPV is divided into three major groups, i.e., high-risk (HR) group, probable high-risk (pHR) group, and low-risk (LR) group according to their potential of causing cervical cancer. Therefore, developing a sensitive, reliable, and cost-effective point-of-care diagnostic method for the virus genotypes in developing countries even worldwide is of high importance...
Source: Analytical and Bioanalytical Chemistry - October 6, 2019 Category: Chemistry Authors: Ma B, Fang J, Lin W, Yu X, Sun C, Zhang M Tags: Anal Bioanal Chem Source Type: research

Intensive optimization and evaluation of global DNA methylation quantification using LC-MS/MS.
Abstract DNA methylation is a typical epigenetic phenomenon. Numerous methods for detecting global DNA methylation levels have been developed, among which LC-MS/MS has emerged as an excellent method from the viewpoint of sensitivity, reproducibility, and cost. However, LC-MS/MS methods have limitations due to a lack of the stability and the standardization required for a laboratory assay. The present study aimed to establish a robust assay that guarantees highly accurate measurements of global DNA methylation levels. There are at least three facets of the developed method. The first is discovery of the solvent con...
Source: Analytical and Bioanalytical Chemistry - October 3, 2019 Category: Chemistry Authors: Nakagawa T, Wakui M, Hayashida T, Nishime C, Murata M Tags: Anal Bioanal Chem Source Type: research

Elemental pub quiz challenge.
PMID: 31506718 [PubMed - in process] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - September 12, 2019 Category: Chemistry Authors: Meija J Tags: Anal Bioanal Chem Source Type: research

Solution to lead quantitation challenge.
PMID: 31506719 [PubMed - in process] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - September 12, 2019 Category: Chemistry Authors: Pagliano E Tags: Anal Bioanal Chem Source Type: research

Multi-residue ultra-performance liquid chromatography coupled with tandem mass spectrometry method for comprehensive multi-class anthropogenic compounds of emerging concern analysis in a catchment-based exposure-driven study.
Abstract This paper presents a new multi-residue method for the quantification of more than 142 anthropogenic compounds of emerging concern (CECs) in various environmental matrices. These CECs are from a wide range of major classes including pharmaceuticals, household, industrial and agricultural. This method utilises ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) for analysis of five matrices (three liquid and two solid) from wastewater treatment processes and the surrounding environment. Relative recoveries were predominantly between 80 and 120%; however, due to the co...
Source: Analytical and Bioanalytical Chemistry - September 7, 2019 Category: Chemistry Authors: Proctor K, Petrie B, Barden R, Arnot T, Kasprzyk-Hordern B Tags: Anal Bioanal Chem Source Type: research

Structural analysis of the neuropeptide substance P by using vibrational spectroscopy.
Abstract Substance P (SP) is one of the most studied peptide hormones and knowing the relationship between its structure and function may have important therapeutic applications in the treatment of a variety of stress-related illnesses. In order to obtain a deeper insight into its folding, the effects of different factors, such as pH changes, the presence of Ca2+ ions, and the substitution of the Met-NH2 moiety in the SP structure, was studied by Raman and infrared spectroscopies. SP has a pH-dependent structure. Under acidic-neutral conditions, SP possesses a prevalent β-sheet structure although also other s...
Source: Analytical and Bioanalytical Chemistry - September 7, 2019 Category: Chemistry Authors: Jurasekova Z, Garcia-Leis A, Sanchez-Cortes S, Tinti A, Torreggiani A Tags: Anal Bioanal Chem Source Type: research

Development of complementary CE-MS methods for speciation analysis of pyrithione-based antifouling agents.
ermann B Abstract In the recent decade, metal pyrithione complexes have become important biocides for antifouling purposes in shipping. The analysis of metal pyrithione complexes and their degradation products/species in environmental samples is challenging because they exhibit fast UV degradation, transmetalation, and ligand substitution and are known to be prone to spontaneous species transformation within a chromatographic system. The environmental properties of the pyrithione species, e.g., toxicity to target and non-target organisms, are differing strongly, and it is therefore inevitable to identify as well a...
Source: Analytical and Bioanalytical Chemistry - September 7, 2019 Category: Chemistry Authors: Faßbender S, Döring AK, Meermann B Tags: Anal Bioanal Chem Source Type: research

Evaluating the biochemical changes of LPS-stimulated endothelial cells by synchrotron FTIR microspectroscopy at a single-cell level.
In this study, the biochemical changes to endothelial cells exposed to LPS were investigated by synchrotron FTIR microspectroscopy at a single-cell level. We found that the whole infrared spectrum of endothelial cells shifted after LPS treatment, indicating chemical component changes within cells. Principal component analysis (PCA) and t tests on subspectra (fatty acid region, protein region, and nucleic acid-sugar region, respectively) further showed that sugar components as well as fatty acids changed dramatically while proteins had no significant variation following LPS exposure. These results suggested that the glycoca...
Source: Analytical and Bioanalytical Chemistry - September 6, 2019 Category: Chemistry Authors: Wang Y, Wang Y, Huang L, Liu X, Hu J, Lü J Tags: Anal Bioanal Chem Source Type: research

Authentication of black cohosh (Actaea racemosa) dietary supplements based on chemometric evaluation of hydroxycinnamic acid esters and hydroxycinnamic acid amides.
Abstract Ester and amide derivatives of hydroxycinnamic acids are found in black cohosh (Actaea racemosa) and other Actaea plants. These two compound groups were evaluated for authentication of black cohosh dietary supplements. The hydroxycinnamic acid esters (HCAE) were profiled by ultra-performance liquid chromatography-photodiode array detection (UPLC-PDA). The hydroxycinnamic acid amides (HCAA) were acquired simultaneously by mass spectrometry-multiple reaction monitoring (UPLC-MRM) mode. In contrast with the traditional HCAE method using 8 compounds, profiles of HCAA using only 4 feruloyl dopamine-O-hexosides...
Source: Analytical and Bioanalytical Chemistry - September 6, 2019 Category: Chemistry Authors: Geng P, Chen P, Sun J, McCoy JH, Harnly JM Tags: Anal Bioanal Chem Source Type: research

Selective fluorescence sensor based on ion-imprinted polymer-modified quantum dots for trace detection of Cr(VI) in aqueous solution.
Abstract A new fluorescence sensor (QDs-IIP), based on ion-imprinted polymers (IIP) as recognition element and Mn-doped ZnS quantum dots (QDs) as fluorophore, was synthesized for the selective and sensitive determination of hexavalent chromium (Cr(VI)) ions. The QD was first coated by SiO2 layer, and then modified with Cr(VI)-imprinted polymer. Several parameters affecting fluorescence intensity such as tetraethylorthosilicate (TEOS) volume, ZnS:Mn@SiO2 amount, and radiation time were investigated and optimized. The QDs-IIP was characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR), transmissi...
Source: Analytical and Bioanalytical Chemistry - September 5, 2019 Category: Chemistry Authors: Zhang MY, Huang RF, Ma XG, Guo LH, Wang Y, Fan YM Tags: Anal Bioanal Chem Source Type: research

Quantitative analysis of PET microplastics in environmental model samples using quantitative 1H-NMR spectroscopy: validation of an optimized and consistent sample clean-up method.
Abstract Identification and quantification of microplastics (MP) in environmental samples is crucial for understanding the risk and distribution of MP in the environment. Currently, quantification of MP particles in environmental samples and the comparability of different matrices is a major research topic. Research also focusses on sample preparation, since environmental samples must be free of inorganic and organic matrix components for the MP analysis. Therefore, we would like to propose a new method that allows the comparison of the results of MP analysis from different environmental matrices and gives a MP co...
Source: Analytical and Bioanalytical Chemistry - September 5, 2019 Category: Chemistry Authors: Peez N, Becker J, Ehlers SM, Fritz M, Fischer CB, Koop JHE, Winkelmann C, Imhof W Tags: Anal Bioanal Chem Source Type: research

Glenn Taylor: The horse who came to dinner: the first criminal case of food fraud.
PMID: 31489441 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - September 5, 2019 Category: Chemistry Authors: Emons H Tags: Anal Bioanal Chem Source Type: research

Analytical and Bioanalytical Chemistry, and the International Year of the Periodic Table.
PMID: 31486866 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - September 5, 2019 Category: Chemistry Authors: Woolley AT Tags: Anal Bioanal Chem Source Type: research

In honor of Philippe Garrigues.
PMID: 31486867 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - September 5, 2019 Category: Chemistry Authors: Wise SA Tags: Anal Bioanal Chem Source Type: research

Identification performance of MALDI-ToF-MS upon mono- and bi-microbial cultures is cell number and culture proportion dependent.
r I Abstract Biotyping using matrix-assisted laser desorption ionization-time of flight (MALDI-ToF) mass spectroscopy (MS) has revolutionized microbiology by allowing clinicians and scientists to rapidly identify microbes at genus and species levels. The present study extensively assesses the suitability and reliability of MALDI-ToF biotyping of 14 different aerobic and anaerobic bacterial species as pure and mixed cultures. Reliable identification at species level was possible from biomaterial of older colonies and even frozen biomaterial, although this was species dependent. Using standard instrument settings an...
Source: Analytical and Bioanalytical Chemistry - September 5, 2019 Category: Chemistry Authors: Mörtelmaier C, Panda S, Robertson I, Krell M, Christodoulou M, Reichardt N, Mulder I Tags: Anal Bioanal Chem Source Type: research

Evaluation of droplet digital PCR for non-invasive prenatal diagnosis of phenylketonuria.
In conclusion, this study is the first to establish non-invasive prenatal diagnosis of PKU based on ddPCR. The method showed high sensitivity and specificity from cfDNA, indicating that ddPCR is a reliable non-invasive prenatal diagnosis tool for PKU diagnosis. Graphical abstract. PMID: 31485704 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - September 4, 2019 Category: Chemistry Authors: Yan Y, Wang F, Zhang C, Jin X, Zhang Q, Feng X, Hao S, Gao H, Ma X Tags: Anal Bioanal Chem Source Type: research

Photoclick chemistry to create dextran-based nucleic acid microarrays.
aquieira Á Abstract In the literature, there are reports of the utilization of various hydrogels to create generic platforms for protein microarray applications. Here, a novel strategy was developed to obtain high-performance microarrays. In it, a dextran hydrogel is used to covalently immobilize oligonucleotides and proteins. This method employs aqueous solutions of dextran methacrylate (Dx-MA), which is a biocompatible photopolymerizable monomer. Capture probes are immobilized inside the hydrogel via a light-induced thiol-acrylate coupling reaction at the same time as the dextran polymer is formed. Hydrog...
Source: Analytical and Bioanalytical Chemistry - September 3, 2019 Category: Chemistry Authors: Díaz-Betancor Z, Bañuls MJ, Maquieira Á Tags: Anal Bioanal Chem Source Type: research

New photothermal immunoassay of human chorionic gonadotropin using Prussian blue nanoparticle-based photothermal conversion.
Abstract A new near-infrared-based photothermal immunosensing strategy was developed for the sensitive and feasible detection of human chorionic gonadotropin (HCG) by use of a Prussian blue nanoparticle-based photothermal conversion system. Prussian blue nanospheres synthesized by the one-pot method were used for the labeling of anti-HCG detection antibody. A sandwich-type immunoreaction was initially conducted on a monoclonal anti-HCG antibody-coated microplate with a nanoparticle-labeled signal antibody. Accompanying formation of the sandwiched immunocomplex, Prussian blue nanospheres caused photothermal convers...
Source: Analytical and Bioanalytical Chemistry - August 30, 2019 Category: Chemistry Authors: Hong G, Zhang D, He Y, Yang Y, Chen P, Yang H, Zhou Z, Liu Y, Wang Y Tags: Anal Bioanal Chem Source Type: research

Quantification of microplastics in environmental samples via pressurized liquid extraction and pyrolysis-gas chromatography.
s T Abstract The quantification of microplastics (MP) in environmental samples is currently a challenging task. To enable low quantification limits, an analytical method has been developed combining pressurized liquid extraction (PLE) and pyrolysis GC-MS. The automated extraction includes a pre-extraction step via methanol followed by a subsequent PLE using tetrahydrofuran. For the most frequently used synthetic polymers polyethylene (PE), polypropylene (PP), and polystyrene (PS), limits of quantification were achieved down to 0.007 mg/g. Recoveries above 80% were attained for solid matrices such as soil and ...
Source: Analytical and Bioanalytical Chemistry - August 30, 2019 Category: Chemistry Authors: Dierkes G, Lauschke T, Becher S, Schumacher H, Földi C, Ternes T Tags: Anal Bioanal Chem Source Type: research

Quantitative evaluation of liposomal doxorubicin and its metabolites in spheroids.
Abstract Accurate measurement and understanding of therapeutic uptake and metabolism is key in the drug development process. This work examines the amount of doxorubicin that can penetrate into spheroids after being encapsulated in a liposomal configuration in comparison with free drug. Through a process known as serial trypsinization, three distinct cellular populations of a spheroid were successfully separated and a small molecule extraction was used to isolate the chemotherapeutic. Doxorubicin showed a time-dependent permeability into spheroids with the most drug accumulating in the core at 24 h of treatme...
Source: Analytical and Bioanalytical Chemistry - August 30, 2019 Category: Chemistry Authors: Lukowski JK, Hummon AB Tags: Anal Bioanal Chem Source Type: research

Construction of a novel cell-trappable fluorescent probe for hydrogen sulfide (H2S) and its bio-imaging application.
Abstract Fluorescence detection of H2S in living organisms is greatly advantageous because it is nondestructive and can be used for in situ analysis. We have constructed a novel rhodamine analogue dye (Rho630) by extending the conjugated system of rhodamine to create a novel cell-trappable H2S fluorescent probe Rho630-AM-H2S with red light emission. Its application for H2S fluorescence detection in living HeLa cells and zebrafish was investigated. As expected, Rho630-AM-H2S showed a huge fluorescence turn-on response of about 20-fold at 630 nm and good selectivity toward H2S in solution. An MTT assay demonstr...
Source: Analytical and Bioanalytical Chemistry - August 29, 2019 Category: Chemistry Authors: Lin X, Chen Y, Wang S, Liu K, Kong F Tags: Anal Bioanal Chem Source Type: research

Challenges of big data integration in the life sciences.
Abstract Big data has been reported to be revolutionizing many areas of life, including science. It summarizes data that is unprecedentedly large, rapidly generated, heterogeneous, and hard to accurately interpret. This availability has also brought new challenges: How to properly annotate data to make it searchable? What are the legal and ethical hurdles when sharing data? How to store data securely, preventing loss and corruption? The life sciences are not the only disciplines that must align themselves with big data requirements to keep up with the latest developments. The large hadron collider, for instance, g...
Source: Analytical and Bioanalytical Chemistry - August 28, 2019 Category: Chemistry Authors: Fillinger S, de la Garza L, Peltzer A, Kohlbacher O, Nahnsen S Tags: Anal Bioanal Chem Source Type: research

Optimal inter-batch normalization method for GC/MS/MS-based targeted metabolomics with special attention to centrifugal concentration.
In conclusion, the TI-based normalization method is the most effective to normalize inter-batch variation for GC/MS/MS-based metabolome analysis. Graphical abstract. PMID: 31463516 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - August 28, 2019 Category: Chemistry Authors: Zaitsu K, Noda S, Ohara T, Murata T, Funatsu S, Ogata K, Ishii A, Iguchi A Tags: Anal Bioanal Chem Source Type: research

Thiol-specific fluorogenic agent for live cell non-protein thiol imaging in lysosomes.
Abstract Thiol molecules play a significant role in cellular structures and functions. These molecules are distributed in cells unevenly at the subcellular level. Disturbance of cellular thiols has been associated with various diseases and disorders. Probes that are able to detect subcellular thiol density in live cells are valuable tools in determining thiols' roles at the subcellular level. Lysosomes are a subcellular organelle involved in the degradation of macromolecules through the action of proteolytic enzymes. The degradation not only serves as a way to dispose of unwanted macromolecules but also a way to r...
Source: Analytical and Bioanalytical Chemistry - August 26, 2019 Category: Chemistry Authors: Alqahtani Y, Wang S, Najmi A, Huang Y, Guan X Tags: Anal Bioanal Chem Source Type: research

Self-propelled micromachines for analytical sensing: a critical review.
chez B, Escarpa A Abstract Self-propelled micromotors are micro- and nanoscale devices that move autonomously in solution by converting a specific stimulus into mechanical work. The broad scope of operations and applications along with the ultra-small dimensions have opened new possibilities to solve complex analytical challenges. Herein we give a critical overview of early developments and future prospects of such tiny moving objects for different analytical sensing and biosensing strategies. From early electrophoretic propelled nanomotors, which were limited to low viscous media, to bubble-propelled micromotors,...
Source: Analytical and Bioanalytical Chemistry - August 24, 2019 Category: Chemistry Authors: Pacheco M, López MÁ, Jurado-Sánchez B, Escarpa A Tags: Anal Bioanal Chem Source Type: research

Citrate-capped gold nanoparticles as a sensing probe for determination of cetyltrimethylammonium surfactant using FTIR spectroscopy and colorimetry.
Abstract A novel, facile, and low-cost method was developed for determination of cetyltrimethylammonium (CTA+) cationic surfactant in water samples using diffuse reflectance Fourier transform IR (FTIR) spectroscopy and colorimetry. Cetyltrimethylammonium bromide was chosen as a model compound to demonstrate the optimization of the method for determination of CTA+ in water samples. The absorption peak at 3015.96 cm-1 (for CTA+) was enhanced when gold nanoparticles were used as a chemical sensor in diffuse reflectance FTIR spectroscopy, and this absorption peak was used for determination of CTA+. Alternatively, the ...
Source: Analytical and Bioanalytical Chemistry - August 23, 2019 Category: Chemistry Authors: Kurrey R, Deb MK, Shrivas K, Khalkho BR, Nirmalkar J, Sinha D, Jha S Tags: Anal Bioanal Chem Source Type: research

A novel GC-MS methodology to evaluate aromatase activity in human placental microsomes: a comparative study with the standard radiometric assay.
Abstract Estrogens are key factors in the development of the estrogen receptor-positive (ER+) breast cancer. Estrogens, estrone (E1), and estradiol (E2) production is achieved by aromatase, a cytochrome P450 enzyme that has androgens, androstenedione (AD), and testosterone (T) as substrates. Nowadays, third-generation aromatase inhibitors (AIs) are considered the gold-standard treatment for ER+ breast cancer in postmenopausal women as well as in premenopausal women with ovary ablation. Aromatase activity assessment still relies on radiometric assays that are expensive, hazardous, and non-environmentally friendly. ...
Source: Analytical and Bioanalytical Chemistry - August 22, 2019 Category: Chemistry Authors: Augusto TV, Cunha SC, Amaral C, Fernandes JO, da Silva ET, Roleira FFM, Teixeira N, Correia-da-Silva G Tags: Anal Bioanal Chem Source Type: research

Use of mTRAQ derivatization reagents on tissues for imaging neurotransmitters by MALDI imaging mass spectrometry: the triple spray method.
Abstract During drug development, matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry is used for visually elucidating the distribution of substances such as biomarkers, candidate compounds, and metabolites in the tissues. However, it is difficult to make relative comparisons between tissue sections and there are still many challenges. Here, we report a new method of "triple spray" for the comparison of analyte distribution in multiple tissue slices. This method targets amino acids and amines, and it incorporates the application of the internal standard in the on-tissue derivati...
Source: Analytical and Bioanalytical Chemistry - August 22, 2019 Category: Chemistry Authors: Ito T, Hiramoto M Tags: Anal Bioanal Chem Source Type: research

Novel probes for label-free detection of neurodegenerative GGGGCC repeats associated with amyotrophic lateral sclerosis.
Abstract DNA repeat expansion sequences cause a myriad of neurological diseases when they expand beyond a critical threshold. Previous electrochemical approaches focused on the detection of trinucleotide repeats (CAG, CGG, and GAA) and relied on labeling of the probe and/or target strands or enzyme-linked assays. However, detection of expanded GC-rich sequences is challenging because they are prone to forming secondary structures such as cruciforms and quadruplexes. Here, we present label-free detection of hexanucleotide GGGGCC repeat sequences, which cause the leading genetic form of frontotemporal dementia (FTD)...
Source: Analytical and Bioanalytical Chemistry - August 21, 2019 Category: Chemistry Authors: Taki M, Rohilla KJ, Barton M, Funneman M, Benzabeh N, Naphade S, Ellerby LM, Gagnon KT, Shamsi MH Tags: Anal Bioanal Chem Source Type: research

Self-heating tilted fiber Bragg grating device for melt curve analysis of solid-phase DNA hybridization and thermal cycling.
Abstract We demonstrate a DNA-based optical fiber device that uses an in-fiber grating, a light absorbing coating with surface anchored DNA, and a built-in optical thermometer. This device is used for precisely thermal cycling surface DNA spots bound by a simple UV cross-linking technique. Near-infrared light of wavelengths near 1550 nm and guided power near 300 mW is coupled out of the fiber core by a tilted fiber Bragg grating inscribed in the fiber and absorbed by the coating to increase its temperature to more than 95 °C. A co-propagating broadband light signal (also in the near-infrared region) is used to...
Source: Analytical and Bioanalytical Chemistry - August 20, 2019 Category: Chemistry Authors: Koppert J, Jean-Ruel H, O'Neill D, Harder C, Willmore W, Ianoul A, Albert J Tags: Anal Bioanal Chem Source Type: research

Graphene oxide composites for magnetic solid-phase extraction of twelve quinolones in water samples followed by MALDI-TOF MS.
Abstract Antibiotic compounds in natural waters are normally present at low concentrations. In this paper, an easy and highly sensitive screening method using graphene oxide-functionalized magnetic composites (GO@NH2@Fe3O4) combined with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was established for twelve quinolone antibiotics. GO@NH2@Fe3O4 composites were utilized as adsorbents for magnetic solid-phase extraction. This method combines the advantages of magnetic solid-phase extraction and MALDI-TOF MS, which allows for fast detection of quinolones at low concentrat...
Source: Analytical and Bioanalytical Chemistry - August 19, 2019 Category: Chemistry Authors: Tang HZ, Wang YH, Li S, Wu J, Li JW, Zhou HY, Gao ZX Tags: Anal Bioanal Chem Source Type: research

Correction to: Baltimore PM2.5 Supersite: highly time-resolved organic compounds-sampling duration and phase distribution-implications for health effects studies.
Abstract The authors would like to call the reader's attention to the fact that unfortunately Orhan Sevimoglu's affiliation was wrong in the original publication. PMID: 31422430 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - August 17, 2019 Category: Chemistry Authors: Rogge WF, Ondov JM, Bernardo-Bricker A, Sevimoglu O Tags: Anal Bioanal Chem Source Type: research

Enhancing signal and mitigating up-front peptide fragmentation using controlled clustering by gas-phase modifiers.
In this study, we introduce gas-phase modifiers (acetonitrile, acetone, cyclohexane, water, and methanol) to the curtain gas to mitigate up-front CID fragmentation. These modifiers cluster with incoming ions, increasing the energy barrier to fragmentation and consequently reducing the complexity of mass spectra. The clustering is monitored by differential mobility spectrometry-mass spectrometry (DMS-MS) and precursor mass spectrum-scanning. Unlike typical singly charged species, peptide ion-modifier clusters were found to survive through the atmosphere-to-vacuum interface of the mass spectrometer, showing that highly charg...
Source: Analytical and Bioanalytical Chemistry - August 17, 2019 Category: Chemistry Authors: Seale B, Schneider BB, Le Blanc JCY Tags: Anal Bioanal Chem Source Type: research

Development and comparison of mimotope-based immunoassays for the analysis of fumonisin B1.
no-Bondi MC Abstract Mycotoxins can be found as natural contaminants in many foods and feeds, and owing to their toxic effects, it is essential to detect them before they enter the food chain. An interesting approach for the analysis of mycotoxins by competitive immunoassays is the use of epitope-mimicking peptides, or mimotopes, which can replace the toxin conjugates traditionally used in such assays. Mimotopes can be selected from phage-displayed peptide libraries even without any prior knowledge of the antibody-antigen interaction, and after identifying the target specific clones, individual clones can be effic...
Source: Analytical and Bioanalytical Chemistry - August 17, 2019 Category: Chemistry Authors: Peltomaa R, Agudo-Maestro I, Más V, Barderas R, Benito-Peña E, Moreno-Bondi MC Tags: Anal Bioanal Chem Source Type: research

Arabidopsis thaliana ITS sequence-specific DNA extraction by ion-tagged oligonucleotides coupled with a magnetic ionic liquid.
This study reports a follow-up investigation on the capture of specific DNA sequences using ion-tagged oligonucleotides (ITOs) and magnetic ionic liquids (MIL). Five allylimidazolium salts bearing octyl substituents ([AOIM+]-ITOs) were used for the selective extraction of the internal transcribed spacer region (ITS) from Arabidopsis thaliana. In this work, the ability of the [AOIM+]-ITOs to enhance the extraction of longer target sequences (~ 700 bp) of plant origin was shown. Moreover, the independence of the probe binding position and the importance of complementarity to the target region for the extraction p...
Source: Analytical and Bioanalytical Chemistry - August 17, 2019 Category: Chemistry Authors: Marengo A, Emaus MN, Bertea CM, Bicchi C, Rubiolo P, Cagliero C, Anderson JL Tags: Anal Bioanal Chem Source Type: research

Signal pattern plot: a simple tool for time-dependent metabolomics studies by 1H NMR spectroscopy.
Abstract We show an alternative way to visualize time course NMR data without the application of multivariate data analysis, based on the temporal change of the metabolome of hazelnuts after mold infestation. Fresh hazelnuts were inoculated with eight different natural mold species and the growth was studied over a period of 14 days. The data were plotted in a color-coded scheme showing metabolic changes as a function of chemical shift, which we named signal pattern plot. This plot graphically displays alteration (trend) of a respected signal over time and allows visual interpretation in a simple manner. Chan...
Source: Analytical and Bioanalytical Chemistry - August 16, 2019 Category: Chemistry Authors: Bachmann R, Jilani A, Ibrahim H, Bahmann D, Lang C, Fischer M, Bisping B, Hackl T Tags: Anal Bioanal Chem Source Type: research

Screening for estrogen active nonylphenols in surface waters by planar solid phase extraction-planar yeast estrogen screen.
This study presents a new sensitive and innovative screening approach for estrogen active NP based on high-performance thin-layer chromatography. NP were focused in a single target zone on thin-layer plates by planar solid phase extraction (pSPE) and detected by a planar yeast estrogen screen (pYES) on the basis of their estrogenic activity. The mean limits of detection and quantitation were 14 and 26 ng per zone, respectively. After liquid-liquid extraction of water samples with dichloromethane, the mean recovery was close to 100% (relative standard deviation of 21% or less), and estrogen active NP were detectable down to...
Source: Analytical and Bioanalytical Chemistry - August 16, 2019 Category: Chemistry Authors: Schick D, Oellig C Tags: Anal Bioanal Chem Source Type: research

Vibrational extraction QuEChERS for analysis of antiparasitic agents in fish by liquid chromatography coupled with tandem mass spectrometry.
her M Abstract A method was developed for the analysis of 22 antiparasitic residues belonging to the benzoylurea, organophosphate, pyrimidinamine, pyrethrin and pyrethroid classes in salmon by liquid chromatography coupled with tandem mass spectrometry. Samples were extracted with acetonitrile-water as the extraction solvent with use of a vibrational shaking apparatus with a ceramic homogenizer. After extraction, the acetonitrile extracts were cleaned up by incubation at low temperature (-20 °C, 1 h) to remove fat, followed by dispersive solid-phase extraction using Z-Sep+ and primary-secondary amine as sorben...
Source: Analytical and Bioanalytical Chemistry - August 16, 2019 Category: Chemistry Authors: de Oliveira LG, Ramkumar A, Moloney M, Kurz MHS, Gonçalves FF, Prestes OD, Danaher M Tags: Anal Bioanal Chem Source Type: research

Fourier transform infrared spectroscopic imaging of colon tissues: evaluating the significance of amide I and C-H stretching bands in diagnostic applications with machine learning.
This study demonstrates, for the first time, that C-H stretching and amide I bands are of little to no significance in the classification of the colon malignancy, based on the Gini importance values by random forest (RF). The best prediction outcome is found when supervised RF classification was carried out in the fingerprint region of the spectral data between 1500 and 1000 cm-1 (excluding the contribution of amide I and II bands). An overall prediction accuracy higher than 90% is achieved through the RF. The results also show that dysplastic and hyperplastic tissues are well distinguished. This leads to the insight ...
Source: Analytical and Bioanalytical Chemistry - August 16, 2019 Category: Chemistry Authors: Song CL, Vardaki MZ, Goldin RD, Kazarian SG Tags: Anal Bioanal Chem Source Type: research