Facile preparation of novel Pd nanowire networks on a polyaniline hydrogel for sensitive determination of glucose.
In this study, novel Pd nanowire networks (PdNW) grown on three-dimensional polyaniline hydrogel (3D-PANI) were prepared via a facile one-step electrodeposition approach at a constant potential of - 0.2 V and further utilized as an electrochemical sensing material for sensitive determination of glucose in alkaline medium. Compared with the sensor based on Pd nanofilm (PdNF)/3D-PANI prepared by electrodeposition at - 0.9 V, the sensor based on PdNW/3D-PANI presented substantially enhanced electrocatalytic activity towards glucose oxidation, with an excellent sensitivity of 146.6 μA mM-1&...
Source: Analytical and Bioanalytical Chemistry - August 1, 2020 Category: Chemistry Authors: Li Z, Qian W, Guo H, Song X, Yan H, Jin R, Zheng J Tags: Anal Bioanal Chem Source Type: research

Development of a certified genomic DNA reference material for detection and quantification of genetically modified rice KMD.
In this study, a batch of genomic DNA (gDNA) certified reference materials (CRMs) was developed using genetically modified rice Kemingdao (KMD) homozygotes as the raw material. The gDNA CRMs in this batch showed good homogeneity; the minimum sample intake was determined to be 2 μL. The stability study showed that transportation by cold chain is preferable, no significant degradation trend was observed during a 12-month period when storing the gDNA CRMs at 4 °C and - 20 °C, and the number of freeze-thaw cycles cannot exceed 10. The property values of the copy number ratio of transgene and en...
Source: Analytical and Bioanalytical Chemistry - August 1, 2020 Category: Chemistry Authors: Li J, Li L, Zhang L, Zhang X, Li X, Zhai S, Gao H, Li Y, Wu G, Wu Y Tags: Anal Bioanal Chem Source Type: research

Phospholipidomics of peripheral blood mononuclear cells (PBMCs): the tricky case of children with autism spectrum disorder (ASD) and their healthy siblings.
Abstract Autism spectrum disorder (ASD) is a broad and heterogeneous group of neurological developmental disorders characterized by impaired social interaction and communication, restricted and repetitive behavioural patterns, and altered sensory processing. Currently, no reliable ASD molecular biomarkers are available. Since immune dysregulation has been supposed to be related with ASD onset and dyslipidaemia has been recognized as an early symptom of biological perturbation, lipid extracts from peripheral blood mononuclear cells (PBMCs), consisting primarily of lymphocytes (T cells, B cells, and NK cells) and mo...
Source: Analytical and Bioanalytical Chemistry - August 1, 2020 Category: Chemistry Authors: Ventura G, Calvano CD, Porcelli V, Palmieri L, De Giacomo A, Xu Y, Goodacre R, Palmisano F, Cataldi TRI Tags: Anal Bioanal Chem Source Type: research

Gas sensors based on mass-sensitive transducers. Part 2: Improving the sensors towards practical application.
Conclusions are then drawn, and development perspectives for gravimetric sensors are discussed. PMID: 32737549 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - July 31, 2020 Category: Chemistry Authors: Oprea A, Weimar U Tags: Anal Bioanal Chem Source Type: research

On-spot quantitative analysis of dicamba in field waters using a lateral flow immunochromatographic strip with smartphone imaging.
Abstract Dicamba herbicide is increasingly used in the world, in particular' with the widespread cultivation of genetically modified dicamba-resistant crops. However, the drift problem in the field has caused phytotoxicity against naive, sensitive crops, raising legal concerns. Thus, it is particularly timely to develop a method that can be used for on-the-spot rapid detection of dicamba in the field. In this paper, a lateral flow immunochromatographic strip (LFIC) was developed. The quantitative detection can be conducted by an app on a smartphone, named "Color Snap." The tool reported here provides res...
Source: Analytical and Bioanalytical Chemistry - July 31, 2020 Category: Chemistry Authors: Qi M, Huo J, Li Z, He C, Li D, Wang Y, Vasylieva N, Zhang J, Hammock BD Tags: Anal Bioanal Chem Source Type: research

SERS spectroscopy using Au-Ag nanoshuttles and hydrophobic paper-based Au nanoflower substrate for simultaneous detection of dual cervical cancer-associated serum biomarkers.
Abstract Ultrasensitive detection of specific biomarkers in clinical serum is helpful for early diagnosis of cervical cancer. In this paper, a surface-enhanced Raman scattering (SERS)-based immunoassay was developed for the simultaneous determination of squamous cell carcinoma antigen (SCCA) and osteopontin (OPN) in cervical cancer serum. Au-Ag nanoshuttles (Au-AgNSs) as SERS tags and hydrophobic filter paper-based Au nanoflowers (AuNFs) as capture substrate were constructed into a sandwich structure which served as an ultrasensitive SERS-based immunoassay platform. Finite difference time domain simulation confirm...
Source: Analytical and Bioanalytical Chemistry - July 31, 2020 Category: Chemistry Authors: Lu D, Ran M, Liu Y, Xia J, Bi L, Cao X Tags: Anal Bioanal Chem Source Type: research

An improved drop casting electrochemical strategy for furosemide quantification in natural waters exploiting chemically reduced graphene oxide on glassy carbon electrodes.
Abstract This work exploits the applicability of a chemically reduced graphene oxide (CRGO) modification on the electrochemical response of a glassy carbon electrode (GCE) for the first-time sensitive determination of furosemide in natural waters. The batch injection analysis (BIA) is proposed as an analytical method, where CRGO-GCE is coupled to a BIA cell for amperometric measurements. Acetate buffer (0.1 μmol L-1, pH 5.2) was used as the background electrolyte. The modification provided an increase in sensitivity (0.024 μA/μmol L-1), low limit of detection (0.7 μmol L-1), RSD (
Source: Analytical and Bioanalytical Chemistry - July 31, 2020 Category: Chemistry Authors: Vasconcelos SC, Rodrigues EM, de Almeida LG, Lepri FG, Pacheco WF, Semaan FS, Dornellas RM Tags: Anal Bioanal Chem Source Type: research

Luigia Sabbatini, Inez Doroth é van der Werf (Eds.): Chemical analysis in cultural heritage.
Luigia Sabbatini, Inez Dorothé van der Werf (Eds.): Chemical analysis in cultural heritage. Anal Bioanal Chem. 2020 Jul 29;: Authors: Wagner B PMID: 32728862 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - July 29, 2020 Category: Chemistry Authors: Wagner B Tags: Anal Bioanal Chem Source Type: research

Determination of neuropeptide Y Y1 receptor antagonist BIBP 3226 and evaluation of receptor expression based on liquid chromatography coupled with tandem mass spectrometry.
Abstract Neuropeptide Y (NPY) is a peptide widely distributed throughout the body that is involved in various physiological processes, including the regulation of feeding behavior and energy homeostasis. 5-Carbamimidamido-2-(2,2-diphenylacetamido)-N-[(4-hydroxyphenyl)methyl]pentanamide (BIBP 3226) is a selective NPY Y1 receptor antagonist with recognized application in bone regeneration studies, requiring quantification at picogram levels. Hence, BIBP 3226 determination is proposed here by a validated HPLC-MS/MS method, based on a reversed-phase Kinetex® core-shell C8 column (2.6 μm, 150 ×...
Source: Analytical and Bioanalytical Chemistry - July 29, 2020 Category: Chemistry Authors: Barreiros L, Silva EMP, Alencastre IS, Lamghari M, Segundo MA Tags: Anal Bioanal Chem Source Type: research

Point-of-care-ready nanoscale ISFET arrays for sub-picomolar detection of cytokines in cell cultures.
Abstract Rapid and frequent screening of cytokines as immunomodulation agents is necessary for precise interventions in severe pathophysiological conditions. In addition to high-sensitivity detection of such analytes in complex biological fluids such as blood, saliva, and cell culture medium samples, it is also crucial to work out miniaturized bioanalytical platforms with potential for high-density integration enabling screening of multiple analytes. In this work, we show a compact, point-of-care-ready bioanalytical platform for screening of cytokines such as interleukin-4 (IL-4) and interleukin-2 (IL-2) based on ...
Source: Analytical and Bioanalytical Chemistry - July 28, 2020 Category: Chemistry Authors: Rani D, Singh Y, Salker M, Vu XT, Ingebrandt S, Pachauri V Tags: Anal Bioanal Chem Source Type: research

Integrated silica membrane-based nucleic acid purification, amplification, and visualization platform for low-cost, rapid detection of foodborne pathogens.
Abstract Real-time fluorescence detection of nucleic acid exhibit excellent performance in analytical and diagnostic applications. However, the requirement of laboratory-based instrument and complex nucleic acid extraction greatly limits their application in point-of-care testing (POCT). Herein, a novel integrated silica membrane-based platform incorporating nucleic acid purification, amplification, and detection steps was developed. A universal and portable visualization platform was fabricated by incorporating denaturation bubble-mediated strand exchange amplification (SEA) reaction with silica membrane. The flu...
Source: Analytical and Bioanalytical Chemistry - July 26, 2020 Category: Chemistry Authors: Wang X, Yan C, Wang X, Zhao X, Shi C, Ma C Tags: Anal Bioanal Chem Source Type: research

Optimization and evaluation of the method for the determination of the manganese content in manganese ores and concentrates as described in ISO 4298:1984.
In this study, a more scientific expression for the principle and reaction and the dosage of pyrophosphate in ISO 4298:1984 are discussed. The pyrophosphate ligand is involved in the oxidation of manganese(II) by potassium permanganate, and plays a role in preventing Mn(II) precipitation or Mn(III) dismutation during the whole reaction. The most appropriate amount of saturated sodium pyrophosphate is 150 mL for the test of manganese ores and concentrates with manganese content from 15% to 60%. The method developed by reducing sample size and reagent consumption was evaluated with ten certified reference materials, sho...
Source: Analytical and Bioanalytical Chemistry - July 25, 2020 Category: Chemistry Authors: Yan C, Liu S, Min H, Li C, Zhu Z, Lu C, Gao Q Tags: Anal Bioanal Chem Source Type: research

Inter-laboratory study of an optimised peptide mapping workflow using automated trypsin digestion for monitoring monoclonal antibody product quality attributes.
, Cook K, Bones J Abstract Peptide mapping analysis is a regulatory expectation to verify the primary structure of a recombinant product sequence and to monitor post-translational modifications (PTMs). Although proteolytic digestion has been used for decades, it remains a labour-intensive procedure that can be challenging to accurately reproduce. Here, we describe a fast and reproducible protocol for protease digestion that is automated using immobilised trypsin on magnetic beads, which has been incorporated into an optimised peptide mapping workflow to show method transferability across laboratories. The complete...
Source: Analytical and Bioanalytical Chemistry - July 25, 2020 Category: Chemistry Authors: Millán-Martín S, Jakes C, Carillo S, Buchanan T, Guender M, Kristensen DB, Sloth TM, Ørgaard M, Cook K, Bones J Tags: Anal Bioanal Chem Source Type: research

Development of a molecular K+ probe for colorimetric/fluorescent/photoacoustic detection of K.
Abstract The potassium ion (K+) plays significant roles in many biological processes. To date, great efforts have been devoted to the development of K+ sensors for colorimetric, fluorescent, and photoacoustic detection of K+ separately. However, the development of molecular K+ probes for colorimetric detection of urinary K+, monitoring K+ fluxes in living cells by fluorescence imaging, and photoacoustic imaging of K+ dynamics in deep tissues still remains an open challenge. Herein, we report the first molecular K+ probe (NK2) for colorimetric, fluorescent, and photoacoustic detection of K+. NK2 is composed of 2-di...
Source: Analytical and Bioanalytical Chemistry - July 25, 2020 Category: Chemistry Authors: Ning J, Lin X, Su F, Sun A, Liu H, Luo J, Wang L, Tian Y Tags: Anal Bioanal Chem Source Type: research

Validation of MALDI-MS imaging data of selected membrane lipids in murine brain with and without laser postionization by quantitative nano-HPLC-MS using laser microdissection.
Abstract MALDI mass spectrometry imaging (MALDI-MSI) is a widely used technique to map the spatial distribution of molecules in sectioned tissue. The technique is based on the systematic generation and analysis of ions from small sample volumes, each representing a single pixel of the investigated sample surface. Subsequently, mass spectrometric images for any recorded ion species can be generated by displaying the signal intensity at the coordinate of origin for each of these pixels. Although easily equalized, these recorded signal intensities, however, are not necessarily a good measure for the underlying amount...
Source: Analytical and Bioanalytical Chemistry - July 25, 2020 Category: Chemistry Authors: Eiersbrock FB, Orthen JM, Soltwisch J Tags: Anal Bioanal Chem Source Type: research

Trends in sample preparation and separation methods for the analysis of very polar and ionic compounds in environmental water and biota samples.
n C Abstract Recent years showed a boost in knowledge about the presence and fate of micropollutants in the environment. Instrumental and methodological developments mainly in liquid chromatography coupled to mass spectrometry hold a large share in this success story. These techniques soon complemented gas chromatography and enabled the analysis of more polar compounds including pesticides but also household chemicals, food additives, and pharmaceuticals often present as traces in surface waters. In parallel, sample preparation techniques evolved to extract and enrich these compounds from biota and water samples. ...
Source: Analytical and Bioanalytical Chemistry - July 24, 2020 Category: Chemistry Authors: Knoll S, Rösch T, Huhn C Tags: Anal Bioanal Chem Source Type: research

Label-free fluorescent sensor for one-step lysozyme detection via positively charged gold nanorods.
Abstract In the article, a simple and label-free strategy was proposed for the sensitive detection of lysozyme based on the fluorescence quenching of positively charged gold nanorods ((+)AuNRs) to DNA-templated silver nanoclusters (DNA/AgNCs). To construct the sensor, a DNA template was designed with a C-rich sequence at the 5'-terminal for the synthesis of AgNCs, while a lysozyme binding aptamer (LBA) at the 3'-terminal for the recognition of lysozyme, and such DNA/AgNCs was used as the fluorescence probe. Meantime, the fluorescence signal of such DNA/AgNCs can be quenched based on the electrostatic adsorption of...
Source: Analytical and Bioanalytical Chemistry - July 23, 2020 Category: Chemistry Authors: Zhang H, Liu P, Wang H, Ji X, Zhao M, Song Z Tags: Anal Bioanal Chem Source Type: research

Two highly sensitive fluorescent probes based on cinnamaldehyde with large Stokes shift for sensing of HSO3- in pure water and living cells.
Abstract Two simple and novel fluorescent probes (CDC1 and CDC2) have been designed and prepared here for sensing HSO3- with large Stokes shifts (about 250 nm). The synthesized probes can react with HSO3- just in 2 min, followed by the obvious color change from blue to colorless. The colorimetric and ratiometric absorbance response of the probes to HSO3- is due to the addition of HSO3- to the electron-deficient C=C double bond group, which prevents significant intramolecular charge transfer (ICT). Besides, CDC1 and CDC2 can detect HSO3- in pure water and detection limits of CDC1 and CDC2 reached 4.59&nbs...
Source: Analytical and Bioanalytical Chemistry - July 23, 2020 Category: Chemistry Authors: Pan X, Cheng S, Zhang C, Qi X Tags: Anal Bioanal Chem Source Type: research

Non-targeted and targeted analysis of oxylipins in combination with charge-switch derivatization by ion mobility high-resolution mass spectrometry.
b NH Abstract Eicosanoids and other oxylipins play an important role in mediating inflammation as well as other biological processes. For the investigation of their biological role(s), comprehensive analytical methods are necessary, which are able to provide reliable identification and quantification of these compounds in biological matrices. Using charge-switch derivatization with AMPP (N-(4-aminomethylphenyl)pyridinium chloride) in combination with liquid chromatography ion mobility quadrupole time-of-flight mass spectrometry (LC-IM-QTOF-MS), we developed a non-target approach to analyze oxylipins in plasma, ser...
Source: Analytical and Bioanalytical Chemistry - July 22, 2020 Category: Chemistry Authors: Hellhake S, Meckelmann SW, Empl MT, Rentmeister K, Wißdorf W, Steinberg P, Schmitz OJ, Benter T, Schebb NH Tags: Anal Bioanal Chem Source Type: research

Comparison of three electromembrane-based extraction systems for NSAIDs analysis in human urine samples.
rnández-Torres R Abstract A comparative study on the extraction efficiency of five non-steroidal anti-inflammatories was carried out using three different electromembrane extraction (EME) devices with different geometries. The employed setups were (a) a hollow fiber configuration (HF-EME), (b) a microfluidic device that allows working in semi-dynamic mode (μF-EME), and (c) a static miniaturized flat membrane device (FM-EME). Each system was applied to the extraction of salicylic acid (SAC), ketoprofen (KTP), naproxen (NAX), diclofenac (DIC), and ibuprofen (IBU) and subsequent determination by high-perfor...
Source: Analytical and Bioanalytical Chemistry - July 21, 2020 Category: Chemistry Authors: Aranda-Merino N, Ramos-Payán M, Callejón-Mochón M, Villar-Navarro M, Fernández-Torres R Tags: Anal Bioanal Chem Source Type: research

Sweat chloride assay by inductively coupled plasma mass spectrometry: a confirmation test for cystic fibrosis diagnosis.
Abstract The current guidelines for sweat chloride analysis identify the procedures for sweat collection, but not for chloride assay, which is usually performed by methods originally not aiming at the low concentrations of chloride found in sweat. To overcome this limitation, we set up, characterized, and adopted an original inductively coupled plasma mass spectrometry (ICP-MS) method for sweat chloride determination, which was designed for its easy use in a clinical laboratory. The method was linear in the range 8.5E-3 to 272.0E-3 mM, precision exhibited a relative standard deviation
Source: Analytical and Bioanalytical Chemistry - July 21, 2020 Category: Chemistry Authors: Marvelli A, Campi B, Mergni G, Di Cicco ME, Turini P, Scardina P, Zucchi R, Pifferi M, Taccetti G, Paolicchi A, la Marca G, Saba A Tags: Anal Bioanal Chem Source Type: research

Scanning electrochemical microscopy and its potential for studying biofilms and antimicrobial coatings.
Abstract Biofilms are known to be well-organized microbial communities embedded in an extracellular polymeric matrix, which supplies bacterial protection against external stressors. Biofilms are widespread and diverse, and despite the considerable large number of publications and efforts reported regarding composition, structure and cell-to-cell communication within biofilms in the last decades, the mechanisms of biofilm formation, the interaction and communication between bacteria are still not fully understood. This knowledge is required to understand why biofilms form and how we can combat them or how we can ta...
Source: Analytical and Bioanalytical Chemistry - July 21, 2020 Category: Chemistry Authors: Caniglia G, Kranz C Tags: Anal Bioanal Chem Source Type: research

Application of pseudotargeted method combined with multivariate statistical analysis for the quality assessment of traditional Chinese medicine preparation, Sanhuang Tablet as a case.
Abstract The quality control of traditional Chinese herbal medicine (TCHM) is considered to be one of the constraints for its modernization and globalization, especially for traditional Chinese medicine preparation (TCMP). The active compounds at high concentration in TCMP are usually selected as markers for qualification and quantification analyses, but we thought these compounds are insufficient to represent the quality of TCMP. For the in-depth investigation, a pseudotargeted analysis method using ultra-high-performance liquid chromatography-quadrupole-orbitrap mass spectrometry operated in the parallel reactio...
Source: Analytical and Bioanalytical Chemistry - July 20, 2020 Category: Chemistry Authors: Li L, Wang Y, Liu S Tags: Anal Bioanal Chem Source Type: research

Nicholas Snow (Ed.): Basic multidimensional gas chromatography.
PMID: 32691083 [PubMed - as supplied by publisher] (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - July 20, 2020 Category: Chemistry Authors: Herrero M Tags: Anal Bioanal Chem Source Type: research

Turn-on detection of glutathione S-transferase based on luminescence resonance energy transfer between near-infrared to near-infrared core-shell upconversion nanoparticles and organic dye.
Abstract Glutathione S-transferase (GST) is a detoxification enzyme of the liver and kidney. Based on the toxicological effect of GST, it is of great significance to develop a rapid and sensitive detection method for GST. In this work, a new luminescence resonance energy transfer (LRET) system has been designed to detect glutathione S-transferase in the near-infrared (NIR) region by utilizing NaGdF4:Yb3+,Tm3+@NaYF4 upconversion nanoparticles (UCNPs) as the donor and NIR dye-806@Glutathione (IR806@GSH) as the acceptor. NaGdF4:Yb3+,Tm3+@NaYF4 UCNPs were synthesized by a coprecipitation method and surface modificatio...
Source: Analytical and Bioanalytical Chemistry - July 20, 2020 Category: Chemistry Authors: Chen H, Yang X, Liu Y, Wang L Tags: Anal Bioanal Chem Source Type: research

Ultrasensitive split-type electrochemical sensing platform for sensitive determination of organophosphorus pesticides based on MnO2 nanoflower-electron mediator as a signal transduction system.
Abstract Organophosphorus pesticides (OPs) are extensively used worldwide as agrochemicals; however, excess use may threaten the health of humans. Thus, it is an urgent need to develop a sensitive method for determination of OPs. Herein, a simple and sensitive split-type electrochemical method was developed by using MnO2 nanoflower-electron mediator as a signal transduction element. The MnO2 nanoflower-electron mediator was synthesized and shows an excellent electrochemical signal attributed to the high specific surface area of MnO2 nanoflower. Meanwhile, the inhibition of OPs on butyrylcholinesterase (BChE) was c...
Source: Analytical and Bioanalytical Chemistry - July 20, 2020 Category: Chemistry Authors: Sun Y, Xiong P, Tang J, Zeng Z, Tang D Tags: Anal Bioanal Chem Source Type: research

Exploring sample preparation and data evaluation strategies for enhanced identification of host cell proteins in drug products of therapeutic antibodies and Fc-fusion proteins.
Abstract Manufacturing of biopharmaceuticals involves recombinant protein expression in host cells followed by extensive purification of the target protein. Yet, host cell proteins (HCPs) may persist in the final drug product, potentially reducing its quality with respect to safety and efficacy. Consequently, residual HCPs are closely monitored during downstream processing by techniques such as enzyme-linked immunosorbent assay (ELISA) or high-performance liquid chromatography combined with tandem mass spectrometry (HPLC-MS/MS). The latter is especially attractive as it provides information with respect to protein...
Source: Analytical and Bioanalytical Chemistry - July 20, 2020 Category: Chemistry Authors: Esser-Skala W, Segl M, Wohlschlager T, Reisinger V, Holzmann J, Huber CG Tags: Anal Bioanal Chem Source Type: research

Chemical profiling of the human skin surface for malaria vector control via a non-invasive sorptive sampler with GC ×GC-TOFMS.
In this study, the chemical differences in the skin surface profiles of 20 human volunteers were compared based on inter-human attractiveness to mosquitoes, as well as inter- and intra-human mosquito biting site preference. A passive, non-invasive approach was followed to sample the wrist and ankle skin surface region. An in-house developed polydimethylsiloxane (PDMS) passive sampler was used to concentrate skin VOCs and semi-VOCs prior to thermal desorption directly in the GC inlet with comprehensive gas chromatography coupled to time-of-flight mass spectrometry (GC×GC-TOFMS). Compounds from a broad range of chemica...
Source: Analytical and Bioanalytical Chemistry - July 18, 2020 Category: Chemistry Authors: Wooding M, Rohwer ER, Naudé Y Tags: Anal Bioanal Chem Source Type: research

A near-infrared excitation/emission fluorescent probe for imaging of endogenous cysteine in living cells and zebrafish.
Abstract The fluorescence imaging technique provides an essential tool for studying biological systems. However, due to the interference of autofluorescence of biological tissues, the application of short-wavelength fluorescent probes in biological imaging was limited. The near-infrared (NIR) excitation/emission fluorescent probe possesses unique advantages in optical imaging in vivo, including less light scattering, minimal photo-damage to biological samples, deep tissue penetration, and weak autofluorescence interference from complicated biological systems. In this work, a convenient fluorophore (E)-2-[2-(6-hydr...
Source: Analytical and Bioanalytical Chemistry - July 17, 2020 Category: Chemistry Authors: Xie R, Li Y, Zhou Z, Pang X, Wu C, Yin P, Li H Tags: Anal Bioanal Chem Source Type: research

Application of molecular SERS nanosensors: where we stand and where we are headed towards?
y D Abstract Molecular specific and highly sensitive detection is the driving force of the surface-enhanced Raman spectroscopy (SERS) community. The technique opens the window to the undisturbed monitoring of cellular processes in situ or to the quantification of small molecular species that do not deliver Raman signals. The smart design of molecular SERS nanosensors makes it possible to indirectly but specifically detect, e.g. reactive oxygen species, carbon monoxide or potentially toxic metal ions. Detection schemes evolved over the years from simple metallic colloidal nanoparticles functionalized with sensing m...
Source: Analytical and Bioanalytical Chemistry - July 16, 2020 Category: Chemistry Authors: Jahn IJ, Mühlig A, Cialla-May D Tags: Anal Bioanal Chem Source Type: research

Effect of ozone stress on the intracellular metabolites from Cobetia marina.
In this study, non-target analyses were performed to identify the metabolites according to the NIST database. As a result, over 170 signals were detected under normal living conditions including 35 potential metabolites. By the comparison of ozone-treated and non-treated samples, five compounds were selected to describe observed trends of signals in the contour plots. Oleic acid exhibited a slight growth by increasing ozone dosage. In contrast, other metabolites such as the amino acid L-proline showed less abundance after ozone treatment, which was more evident once ozone dosage was raised. Thus, this work could provide a ...
Source: Analytical and Bioanalytical Chemistry - July 16, 2020 Category: Chemistry Authors: Li J, Rumancev C, Lutze HV, Schmidt TC, Rosenhahn A, Schmitz OJ Tags: Anal Bioanal Chem Source Type: research

A novel alkaline phosphatase activity sensing strategy combining enhanced peroxidase-mimetic feature of sulfuration-engineered CoOx with electrostatic aggregation.
Abstract Given alkaline phosphatase (ALP) takes part in the phosphorylation/dephosphorylation processes in the body, its activity is universally taken as an important indicator of many diseases, and thus developing reliable and efficient methods for ALP activity determination becomes quite important. Here, we propose a new sensing strategy for ALP activity by integrating the improved peroxidase-mimicking catalysis of sulfuration-engineered CoOx with the hexametaphosphate ion (HMPi)-mediated electrostatic aggregation. After sulfuration engineering, the CoOx composite coming from the pyrolysis of ZIF-67 exhibits enh...
Source: Analytical and Bioanalytical Chemistry - July 16, 2020 Category: Chemistry Authors: Song H, Niu X, Ye K, Wang L, Xu Y, Peng Y Tags: Anal Bioanal Chem Source Type: research

Ratiometric and sensitive cyanide sensing using dual-emissive gold nanoclusters.
Abstract The detection of cyanide anion (CN-), a highly toxic pollutant, has attracted growing attention in the past years. In this work, a nanosensor composed of hyperbranched polyethyleneimine (hPEI)-assisted dual-emissive gold nanoclusters (DE-Au NCs) is proposed for ratiometric detection of CN- based on surface valence state-driving etch. The ratiometric color change of fluorescence is based on a fact that the red-emissive Au NCs with a high content of surface Au(I) can be easily etched by CN-, while the blue-emissive Au NCs with nearly neutral character can resist CN-. Because of the specific gold-CN- chemist...
Source: Analytical and Bioanalytical Chemistry - July 14, 2020 Category: Chemistry Authors: Yang H, Yang Y, Liu S, Zhan X, Zhou H, Li X, Yuan Z Tags: Anal Bioanal Chem Source Type: research

Simultaneous voltammetric detection of glucose and lactate fluctuations in rat striatum evoked by electrical stimulation of the midbrain.
Abstract Glucose and lactate provide energy for cellular function in the brain and serve as an important carbon source in the synthesis of a variety of biomolecules. Thus, there is a critical need to quantitatively monitor these molecules in situ on a time scale commensurate with neuronal function. In this work, carbon-fiber microbiosensors were coupled with fast-scan cyclic voltammetry to monitor glucose and lactate fluctuations at a discrete site within rat striatum upon electrical stimulation of the midbrain projection to the region. Systematic variation of stimulation parameters revealed the distinct dynamics ...
Source: Analytical and Bioanalytical Chemistry - July 14, 2020 Category: Chemistry Authors: Forderhase AG, Styers HC, Lee CA, Sombers LA Tags: Anal Bioanal Chem Source Type: research

Simple and ultrasensitive electrochemical sensor for oxalic acid detection in real samples by one step co-electrodeposition strategy.
Abstract Oxalic acid (OA), naturally available in vegetables and foodstuffs derived from them, easily combines with calcium and iron to form insoluble oxalates. Their chelation will result in various renal diseases; thus, the accurate determination of OA is quite significant in the evaluation of food quality and healthcare settings. Here, we developed an electrochemically induced alcohol-free sol-gel method to obtain platinum nanoparticles (PtNPs) adhered with porous silica on glassy carbon electrode (PSiO2-PtNPs/GCE) by a one-step process, which can be potentially used as an excellent catalyst towards electrochem...
Source: Analytical and Bioanalytical Chemistry - July 13, 2020 Category: Chemistry Authors: Fang Y, Xu X, Guo X, Cui B, Wang L Tags: Anal Bioanal Chem Source Type: research

Nuclear magnetic resonance and surface-assisted laser desorption/ionization mass spectrometry-based serum metabolomics of kidney cancer.
In this study, comprehensive high-resolution proton nuclear magnetic resonance spectroscopy (1H NMR) and silver-109 nanoparticle-enhanced steel target laser desorption/ionization mass spectrometry (109AgNPET LDI MS) approaches were conducted, in conjunction with multivariate data analysis, to discriminate the global serum metabolic profiles of kidney cancer (n = 50) and healthy volunteers (n = 49). Eight potential biomarkers have been identified using 1H NMR metabolomics and nine mass spectral features which differed significantly (p  0.7), area under the receiver operative charact...
Source: Analytical and Bioanalytical Chemistry - July 13, 2020 Category: Chemistry Authors: Nizioł J, Ossoliński K, Tripet BP, Copié V, Arendowski A, Ruman T Tags: Anal Bioanal Chem Source Type: research

Silanized carbon dot-based thermo-sensitive molecularly imprinted fluorescent sensor for bovine hemoglobin detection.
Abstract Using the surface molecular imprinting technique, a thermo-sensitive molecularly imprinted fluorescent sensor was constructed for bovine hemoglobin (BHb) detection with the silanized carbon dots (CD@SiO2) as fluorescent signal, N-isopropylacrylamide as monomer sensitive to temperature, and BHb as template. The silanized carbon dots coated by the molecularly imprinted polymer (CD@SiO2@MIP) were characterized by high-resolution transmission electron microscopy, Fourier transform infrared spectroscopy, and fluorescence spectroscopy. Owing to the combination of the strong fluorescence sensitivity of CDs and t...
Source: Analytical and Bioanalytical Chemistry - July 11, 2020 Category: Chemistry Authors: Zhao Y, Chen Y, Fang M, Tian Y, Bai G, Zhuo K Tags: Anal Bioanal Chem Source Type: research

Analysis of flavonoids with unified chromatography-electrospray ionization mass spectrometry-method development and application to compounds of pharmaceutical and cosmetic interest.
est C Abstract In this project, we aimed at analysing flavonoid-type compounds with unified chromatography (joining supercritical fluid chromatography and enhanced fluidity liquid chromatography with carbon dioxide-methanol mobile phases covering a wide range of compositions) and diode-array and electrospray ionization mass spectrometric detection (UC-DAD-ESI-MS). First, the chromatographic method was developed for 9 standard flavonoid molecules from three different families (flavanols, flavanones and flavonols, glycosylated or not), with a strong focus on mobile phase composition to achieve the elution of a wide ...
Source: Analytical and Bioanalytical Chemistry - July 10, 2020 Category: Chemistry Authors: Molineau J, Meunier M, Noireau A, Fougère L, Petit AM, West C Tags: Anal Bioanal Chem Source Type: research

A multi-residue method by supercritical fluid chromatography coupled with tandem mass spectrometry method for the analysis of chiral and non-chiral chemicals of emerging concern in environmental samples.
Abstract This manuscript presents the development, validation and application of a multi-residue supercritical fluid chromatography coupled with tandem mass spectrometry method for the analysis of 140 chiral and non-chiral chemicals of emerging concern in environmental samples, with 81 compounds being fully quantitative, 14 semi-quantitative and 45 qualitative, validated according to European Medicine Agency (EMA) guidelines (European Medicines Agency 2019). One unified LC-MS method was used to analyse all analytes, which were split into three injection methods to ensure sufficient peak resolution. The unified met...
Source: Analytical and Bioanalytical Chemistry - July 9, 2020 Category: Chemistry Authors: Rice J, Lubben A, Kasprzyk-Hordern B Tags: Anal Bioanal Chem Source Type: research

A fast and simple SPE-LC-MS/MS procedure for extraction and quantitative analysis of 1,2,4-triazole, N,N-dimethylsulfamide, and other small polar organic compounds in groundwater.
Abstract Small polar organic pollutants have been discovered to be great threats to the groundwater' as they often are highly mobile and persistent in the environment. 1,2,4-Triazole and N,N-dimethylsulfamide, two well-known examples of small polar compounds, are frequent pollutants of upper groundwater. Both are degradation products of several fungicides commonly or previously used in agriculture' but also in wood-preserving paints. A common trait in the analysis of these small polar compounds is the lack of sufficient pre-concentration methods to lower the limit of detection and enable quantitative analysis at n...
Source: Analytical and Bioanalytical Chemistry - July 9, 2020 Category: Chemistry Authors: Bollmann UE, Badawi N Tags: Anal Bioanal Chem Source Type: research

Development and validation of a gas chromatography-mass spectrometry method to analyze octanoate enrichments at low concentrations in human plasma.
Abstract A new method for accurately analyzing octanoate enrichment in plasma was developed and validated. Samples were derivatized directly in plasma by transesterification with isobutanol and were analyzed by gas chromatography-mass spectrometry (GC-MS). This method was developed to analyze the precursor enrichment in a stable isotope tracer protocol. Glyceryl tri[1,2,3,4-13C4] octanoate, a stable isotope-labeled medium-chain triglyceride (MCT), was orally administered in combination with (1) exclusively MCT or (2) a combination of protein, carbohydrates, and MCT to investigate the metabolic route of oral MCT un...
Source: Analytical and Bioanalytical Chemistry - July 9, 2020 Category: Chemistry Authors: van Harskamp D, Knottnerus SJG, Visser G, van Goudoever JB, Schierbeek H Tags: Anal Bioanal Chem Source Type: research

An off-on electrochemiluminescence detection for microRNAs based on TiO2 nanotubes sensitized with gold nanoparticles as enhanced emitters.
Abstract A sensitive electrochemiluminescence (ECL) assay for microRNAs (miRNAs) based on a semiconductor nanomaterial sensitized with noble-metal Au nanoparticles (NPs) is successfully developed. TiO2 nanotubes (NTs) were equipped with Au NPs to obtain an enhanced ECL emitter. Then, an ECL assay for miRNA-21 was fabricated, which was based on the use of probe 2 DNA-functionalized Pt/PAMAM nanocomposites (NCs) assembled on the surface of Au/TiO2 NT conjugate via DNA hybridization between probe 1 DNA and capture DNA. The Pt/PAMAM NCs act as an ECL quencher of Au/TiO2 NTs via resonance energy transfer. After the bin...
Source: Analytical and Bioanalytical Chemistry - July 9, 2020 Category: Chemistry Authors: Dai P, Ke J, Xie C, Wei L, Zhang Y, He Y, Chen L, Jin J Tags: Anal Bioanal Chem Source Type: research

Identification and semi-relative quantification of intact glycoforms by nano-LC-(Orbitrap)MS: application to the α-subunit of human chorionic gonadotropin and follicle-stimulating hormone.
Identification and semi-relative quantification of intact glycoforms by nano-LC-(Orbitrap)MS: application to the α-subunit of human chorionic gonadotropin and follicle-stimulating hormone. Anal Bioanal Chem. 2020 Jul 08;: Authors: Al Matari A, Combès A, Camperi J, Fournier T, Pichon V, Delaunay N Abstract Human chorionic gonadotropin (hCG) and follicle-stimulating hormone (FSH) belong to the family of glycoprotein polypeptide hormones called gonadotropins. They are heterodimers sharing the α-subunit structure that has 2 N-glycosylation sites. A method based on nano-reversed-phase li...
Source: Analytical and Bioanalytical Chemistry - July 8, 2020 Category: Chemistry Authors: Al Matari A, Combès A, Camperi J, Fournier T, Pichon V, Delaunay N Tags: Anal Bioanal Chem Source Type: research

Recent advances in optical aptasensor technology for amplification strategies in cancer diagnostics.
Abstract Aptamers are chemically synthetic single-stranded DNA or RNA molecules selected by molecular evolution. They have been widely used as attractive tools in biosensing and bioimaging because they can bind to a large variety of targets with high sensitivity and high affinity and specificity. As recognition elements, aptamers contribute in particular to cancer diagnostics by recognizing different cancer biomarkers, while they can also facilitate ultrasensitive detection by further employing signal amplification elements. Optical techniques have been widely used for direct and real-time monitoring of cancer-rel...
Source: Analytical and Bioanalytical Chemistry - July 8, 2020 Category: Chemistry Authors: Kou X, Zhang X, Shao X, Jiang C, Ning L Tags: Anal Bioanal Chem Source Type: research

Immunochromatographic detection of human hemoglobin from deteriorated bloodstains due to methamphetamine contamination, aging, and heating.
Abstract Japanese police conduct highly sensitive and quick blood tests to detect human hemoglobin (Hb), because bloodstains left at a crime scene have probative value of circumstantial evidence in a criminal investigation. Although DNA detection from a bloodstain is a useful tool to identify an individual, doing so requires evidence that the bloodstain is of human origin. Stimulant drug abuse and dependence causes major social problems and crimes in Japan, and bloodstains are often found inside syringes seized from drug abusers. In this case, Hb often cannot be detected by conventional testing as high concentrati...
Source: Analytical and Bioanalytical Chemistry - July 8, 2020 Category: Chemistry Authors: Murahashi M, Makinodan M, Yui M, Hibi T, Kobayashi M Tags: Anal Bioanal Chem Source Type: research

A SERS-colorimetric dual-mode aptasensor for the detection of cancer biomarker MUC1.
Abstract Human mucin-1 (MUC1) has attracted considerable attention owing to its overexpression in diverse malignancies. Here, for the rapid and efficient detection of MUC1, we present a SERS-colorimetric dual-mode aptasensor, by integrating SERS probes with magnetic separation, which has several distinctive advantages. Using such a dual-mode aptasensor, the colorimetric functionality is distinguishable by the naked eye, providing a fast and straightforward screening ability for the detection of MUC1. Moreover, SERS-based detection greatly improves the detection sensitivity, reaching a limit of detection of 0.1&nbs...
Source: Analytical and Bioanalytical Chemistry - July 6, 2020 Category: Chemistry Authors: Li N, Zong S, Zhang Y, Wang Z, Wang Y, Zhu K, Yang K, Wang Z, Chen B, Cui Y Tags: Anal Bioanal Chem Source Type: research

Dopamine-induced photoluminescence quenching of bovine serum albumin-capped manganese-doped zinc sulphide quantum dots.
Abstract The direct detection of dopamine (DA) in human body fluids is a great challenge for medical diagnostics of neurological disorders like Parkinson's disease, Alzheimer's disease, senile dementia, and schizophrenia. In this work, a simple and turn off luminescence sensing of DA based on bovine serum albumin (BSA)-capped manganese-doped zinc sulphide quantum dots (Mn:ZnS/BSA QDs) is developed. The Mn:ZnS/BSA QDs were synthesized by a chemical co-precipitation method. Due to the special interaction of DA with BSA and metal ions, Mn:ZnS/BSA QDs can serve as an effective sensing platform for DA. The luminescence...
Source: Analytical and Bioanalytical Chemistry - July 6, 2020 Category: Chemistry Authors: Abha K, Sumithra IS, Suji S, Anjana RR, Anjali Devi JS, Nebu J, Lekha GM, Aparna RS, George S Tags: Anal Bioanal Chem Source Type: research

Assessing nanoparticle colloidal stability with single-particle inductively coupled plasma mass spectrometry (SP-ICP-MS).
Abstract Biological interactions, toxicity, and environmental fate of engineered nanoparticles are affected by colloidal stability and aggregation. To assess nanoparticle aggregation, analytical methods are needed that allow quantification of individual nanoparticle aggregates. However, most techniques used for nanoparticle aggregation analysis are limited to ensemble measurements or require harsh sample preparation that may introduce artifacts. An ideal method would analyze aggregate size in situ with single-nanoparticle resolution. Here, we established and validated single-particle inductively coupled plasm...
Source: Analytical and Bioanalytical Chemistry - July 6, 2020 Category: Chemistry Authors: Donahue ND, Francek ER, Kiyotake E, Thomas EE, Yang W, Wang L, Detamore MS, Wilhelm S Tags: Anal Bioanal Chem Source Type: research

Double bond localization in unsaturated rhamnolipid precursors 3-(3-hydroxyalkanoyloxy)alkanoic acids by liquid chromatography-mass spectrometry applying online Patern ò-Büchi reaction.
Double bond localization in unsaturated rhamnolipid precursors 3-(3-hydroxyalkanoyloxy)alkanoic acids by liquid chromatography-mass spectrometry applying online Paternò-Büchi reaction. Anal Bioanal Chem. 2020 Jul 05;: Authors: Jeck V, Froning M, Tiso T, Blank LM, Hayen H Abstract Lipids are biomolecules with a broad variety of chemical structures, which renders them essential not only for various biological functions but also interestingly for biotechnological applications. Rhamnolipids are microbial glycolipids with surface-active properties and are widely used biosurfactants. They are co...
Source: Analytical and Bioanalytical Chemistry - July 5, 2020 Category: Chemistry Authors: Jeck V, Froning M, Tiso T, Blank LM, Hayen H Tags: Anal Bioanal Chem Source Type: research

An effective approach for size characterization and mass quantification of silica nanoparticles in coffee creamer by AF4-ICP-MS.
Abstract Silicon dioxide (SiO2) has been used as a food additive (E551) for decades. However, some safety concerns have been raised recently due to the detection of silica nanoparticles (SiO2 NPs) in a variety of foodstuffs and their unknown long-term health risk to humans. In order for risk assessment to be conducted, it is essential to establish a reliable, valid, and pragmatic method for analysis of SiO2 NPs in foods for estimation of exposure. This paper presents an effective approach for both size characterization and mass quantification of SiO2 NPs in commercial high-fat coffee creamer using asymmetric flow ...
Source: Analytical and Bioanalytical Chemistry - July 4, 2020 Category: Chemistry Authors: Li B, Chua SL, Ch'ng AL, Yu D, Koh SP, Phang H, Chiew PKT Tags: Anal Bioanal Chem Source Type: research