From urban dust and marine sediment to Ginkgo biloba and human serum-a top ten list of Standard Reference Materials (SRMs)
Anal Bioanal Chem. 2021 Jul 21. doi: 10.1007/s00216-021-03527-w. Online ahead of print.ABSTRACTDuring the past 40 years, the National Institute of Standards and Technology (NIST) has developed over 180 natural matrix Standard Reference Materials® (SRMs) for the determination of trace organic constituents in environmental, clinical, food, and dietary supplement matrices. A list of the Top Ten SRMs intended for organic analysis was identified based on selection criteria including analytical challenge to assign certified values, challenges in material preparation, novel matrices, longevity, widespread use, and unique desi...
Source: Analytical and Bioanalytical Chemistry - July 22, 2021 Category: Chemistry Authors: Stephen A Wise Source Type: research

Understanding the electrospray ionization response factors of per- and poly-fluoroalkyl substances (PFAS)
In this study, a method for predicting the behavior of individual PFAS compounds, including relative retention order in chromatography, is presented which is simply based on the number of fluorine atoms in the molecule as well as the class of the compound (e.g., perfluroalkylcarboxylic acids) vs. computational estimations (e.g., non-polar surface area and logP).PMID:34291300 | DOI:10.1007/s00216-021-03545-8 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - July 22, 2021 Category: Chemistry Authors: Jeffrey R Enders Grace M O'Neill Jerry L Whitten David C Muddiman Source Type: research

Simple and fast colorimetric and electrochemical methods for the ultrasensitive detection of glucose
In this study, Amplex Red (AR), as both indicator and mediator, was investigated to detect glucose in presence of glucose oxidase (GOx) enzymes using colorimetric and electrochemical methods. Without using any advanced techniques and sophisticated nanomaterials, 1 μM glucose can be easily detected through simply detecting the solution color with a visual colorimetric method. On the other hand, the electrochemical method can provide much higher sensitivity for the detection of glucose, which achieves a linear range spanning from 20 nM to 3.56 μM with a limit of 7.3 nM (signal-to-noise ratio SNR = 3). It is also found ...
Source: Analytical and Bioanalytical Chemistry - July 22, 2021 Category: Chemistry Authors: Xianchang Li Chengbo Li Shiding Zhang Chaojun Cui Jianxin Li Qianqian Gao Source Type: research

A novel ESIPT fluorescent probe derived from 3-hydroxyphthalimide for hydrazine detection in aqueous solution and living cells
Anal Bioanal Chem. 2021 Jul 21. doi: 10.1007/s00216-021-03530-1. Online ahead of print.ABSTRACTHydrazine is a highly toxic and flammable liquid that can damage human liver, kidney, and central nervous system. Therefore, it is valuable to seek a quick and sensitive method for hydrazine detection in environmental and biological science. Herein, a new fluorescent probe derived from 3-hydroxyphthalimide was synthesized. This probe can rapidly and selectively detect hydrazine with a low detection limit of 4.3 × 10-7 M. The recognition principle is based on hydrazine-induced acetyl deprotection and excited-state intramolec...
Source: Analytical and Bioanalytical Chemistry - July 22, 2021 Category: Chemistry Authors: Shengli Hu Junyu Wang Meiling Luo Zhou Wu Yaoyao Hou Xinpeng Chen Source Type: research

Dynamic evaluation of the protective effect of Dendrobium officinale polysaccharide on acute alcoholic liver injury mice in vitro and in vivo by NIR fluorescence imaging
This study may also provide a potential NIR imaging agent for the clinical research to improve the management of liver injury-related diseases.PMID:34291303 | DOI:10.1007/s00216-021-03546-7 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - July 22, 2021 Category: Chemistry Authors: Gang Nie Yu Zhang Zhihong Zhou Jingya Xu Huiling Wang Dugang Chen Kaiping Wang Source Type: research

Correction to: Synthesis and application of stationary phase for DNA-affinity chromatographic analysis of unmodified and antisense oligonucleotide
Anal Bioanal Chem. 2021 Jul 22. doi: 10.1007/s00216-021-03544-9. Online ahead of print.NO ABSTRACTPMID:34291304 | DOI:10.1007/s00216-021-03544-9 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - July 22, 2021 Category: Chemistry Authors: Sylwia Studzi ńska Ewelina Zawadzka Szymon Bocian Micha ł Szumski Source Type: research

Feature-based molecular networking for identification of organic micropollutants including metabolites by non-target analysis applied to riverbank filtration
In this study, sartans and TP were investigated and grouped regarding their removal behavior under different redox conditions and seasons for the first time. Antihypertensives were grouped into compounds being well removed during riverbank filtration, those primarily removed under anoxic conditions, and rather persistent compounds. Observed seasonal variations were mainly limited to varying river water concentrations. FBMN is a powerful tool for identifying previously unknown or unexpected compounds and their TP in water samples by non-target analysis.PMID:34286355 | DOI:10.1007/s00216-021-03500-7 (Source: Analytical and B...
Source: Analytical and Bioanalytical Chemistry - July 21, 2021 Category: Chemistry Authors: Daniela Oberleitner Robin Schmid Wolfgang Schulz Axel Bergmann Christine Achten Source Type: research

Candidate reference measurement procedure based on HPAEC-PAD for the development of certified reference materials for monosaccharides in serum
Anal Bioanal Chem. 2021 Jul 20. doi: 10.1007/s00216-021-03537-8. Online ahead of print.ABSTRACTTo achieve the measurement reliability of monosaccharides used as diagnostic markers in clinical fields, it is essential to establish certified reference materials (CRMs). The purpose of this study is to develop a serum CRM by adopting high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) as a new candidate reference measurement procedure for the measurement of glucose and galactose, common diagnostic markers of diabetes and galactosemia, respectively. Using various monosaccharides as inter...
Source: Analytical and Bioanalytical Chemistry - July 21, 2021 Category: Chemistry Authors: Min-Seon Seo Ji-Seon Jeong Mariam M Abady Ha-Jeong Kwon Source Type: research

Simultaneous determination of 137 drugs of abuse, new psychoactive substances, and novel synthetic opioids in meconium by UHPLC-QTOF
Anal Bioanal Chem. 2021 Jul 20. doi: 10.1007/s00216-021-03533-y. Online ahead of print.ABSTRACTNew psychoactive substances (NPS) have been introduced into the market in recent years, with new analytes reported every year. The use of these substances in women can occur at any stage of life, even in the childbearing age. Drug use during pregnancy presents significant risks for the mother and the fetus, so it is important to have tools that allow to detect prenatal exposure to these substances of abuse. Therefore, an analytical method for the determination of 137 NPS and other drugs of abuse in meconium by UHPLC-QTOF was deve...
Source: Analytical and Bioanalytical Chemistry - July 21, 2021 Category: Chemistry Authors: Ángela López-Rabuñal Daniele Di Corcia Eleonora Amante Marta Massano Angelines Cruz-Landeira Ana de-Castro-R íos Alberto Salomone Source Type: research

Development and certification of a reference material for zearalenone in maize germ oil
Anal Bioanal Chem. 2021 Jul 21. doi: 10.1007/s00216-021-03532-z. Online ahead of print.ABSTRACTZearalenone (ZEN), an estrogenic mycotoxin produced by several species of Fusarium fungi, is a common contaminant of cereal-based food worldwide. Due to frequent occurrences associated with high levels of ZEN, maize oil is a particular source of exposure. Although a European maximum level for ZEN in maize oil exists according to Commission Regulation (EC) No. 1126/2007 along with a newly developed international standard method for analysis, certified reference materials (CRM) are still not available. To overcome this lack, the fi...
Source: Analytical and Bioanalytical Chemistry - July 21, 2021 Category: Chemistry Authors: Juliane Riedel Sebastian Recknagel Diana Sassenroth Tatjana Mauch Sabine Buttler Thomas Sommerfeld Sibylle Penk Matthias Koch Source Type: research

Feature-based molecular networking for identification of organic micropollutants including metabolites by non-target analysis applied to riverbank filtration
In this study, sartans and TP were investigated and grouped regarding their removal behavior under different redox conditions and seasons for the first time. Antihypertensives were grouped into compounds being well removed during riverbank filtration, those primarily removed under anoxic conditions, and rather persistent compounds. Observed seasonal variations were mainly limited to varying river water concentrations. FBMN is a powerful tool for identifying previously unknown or unexpected compounds and their TP in water samples by non-target analysis.PMID:34286355 | DOI:10.1007/s00216-021-03500-7 (Source: Analytical and B...
Source: Analytical and Bioanalytical Chemistry - July 21, 2021 Category: Chemistry Authors: Daniela Oberleitner Robin Schmid Wolfgang Schulz Axel Bergmann Christine Achten Source Type: research

Candidate reference measurement procedure based on HPAEC-PAD for the development of certified reference materials for monosaccharides in serum
Anal Bioanal Chem. 2021 Jul 20. doi: 10.1007/s00216-021-03537-8. Online ahead of print.ABSTRACTTo achieve the measurement reliability of monosaccharides used as diagnostic markers in clinical fields, it is essential to establish certified reference materials (CRMs). The purpose of this study is to develop a serum CRM by adopting high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) as a new candidate reference measurement procedure for the measurement of glucose and galactose, common diagnostic markers of diabetes and galactosemia, respectively. Using various monosaccharides as inter...
Source: Analytical and Bioanalytical Chemistry - July 21, 2021 Category: Chemistry Authors: Min-Seon Seo Ji-Seon Jeong Mariam M Abady Ha-Jeong Kwon Source Type: research

Simultaneous determination of 137 drugs of abuse, new psychoactive substances, and novel synthetic opioids in meconium by UHPLC-QTOF
Anal Bioanal Chem. 2021 Jul 20. doi: 10.1007/s00216-021-03533-y. Online ahead of print.ABSTRACTNew psychoactive substances (NPS) have been introduced into the market in recent years, with new analytes reported every year. The use of these substances in women can occur at any stage of life, even in the childbearing age. Drug use during pregnancy presents significant risks for the mother and the fetus, so it is important to have tools that allow to detect prenatal exposure to these substances of abuse. Therefore, an analytical method for the determination of 137 NPS and other drugs of abuse in meconium by UHPLC-QTOF was deve...
Source: Analytical and Bioanalytical Chemistry - July 21, 2021 Category: Chemistry Authors: Ángela López-Rabuñal Daniele Di Corcia Eleonora Amante Marta Massano Angelines Cruz-Landeira Ana de-Castro-R íos Alberto Salomone Source Type: research

Analysis of microplastics in drinking water and other clean water samples with micro-Raman and micro-infrared spectroscopy: minimum requirements and best practice guidelines
Anal Bioanal Chem. 2021 Jul 20. doi: 10.1007/s00216-021-03498-y. Online ahead of print.ABSTRACTMicroplastics are a widespread contaminant found not only in various natural habitats but also in drinking waters. With spectroscopic methods, the polymer type, number, size, and size distribution as well as the shape of microplastic particles in waters can be determined, which is of great relevance to toxicological studies. Methods used in studies so far show a huge diversity regarding experimental setups and often a lack of certain quality assurance aspects. To overcome these problems, this critical review and consensus paper o...
Source: Analytical and Bioanalytical Chemistry - July 20, 2021 Category: Chemistry Authors: Darena Schymanski Barbara E O ßmann Nizar Benismail Kada Boukerma Gerald Dallmann Elisabeth von der Esch Dieter Fischer Franziska Fischer Douglas Gilliland Karl Glas Thomas Hofmann Andrea K äppler S ílvia Lacorte Julie Marco Maria El Rakwe Jana Weisser Source Type: research

Ion-exchanged glass microrods as hybrid SERS/fluorescence substrates for molecular beacon-based DNA detection
Anal Bioanal Chem. 2021 Jul 19. doi: 10.1007/s00216-021-03418-0. Online ahead of print.ABSTRACTIon-exchange in molten nitrate salts containing metal ions (i.e. silver, copper, etc.) represents a well-established technique able to modify the chemical-physical properties of glass materials. It is widely used not only in the field of integrated optics (IO) but also, more recently, in plasmonics due to the possibility to induce the formation of metal nanoparticles in the glass matrix by an ad hoc thermal post-process. In this work, the application of this technology for the realisation of low-cost and stable surface-enhanced R...
Source: Analytical and Bioanalytical Chemistry - July 19, 2021 Category: Chemistry Authors: Simone Berneschi Cristiano D'Andrea Francesco Baldini Martina Banchelli Marella de Angelis Stefano Pelli Roberto Pini Diego Pugliese Nadia G Boetti Davide Janner Daniel Milanese Ambra Giannetti Paolo Matteini Source Type: research

Development and validation of an LC-MS/MS assay for the quantification of allopregnanolone and its progesterone-derived isomers, precursors, and cortisol/cortisone in pregnancy
Anal Bioanal Chem. 2021 Jul 19. doi: 10.1007/s00216-021-03523-0. Online ahead of print.ABSTRACTNeuroactive steroids are potent neuromodulators that play a critical role in both maternal and fetal health during pregnancy. These stress-responsive compounds are reportedly low in women with perinatal depression and may be associated with poor pregnancy outcomes in animal models. Chronic stress is a risk factor for adverse birth outcomes. Simultaneous quantification of neuroactive steroids, in combination with stress hormones cortisol/cortisone, provides an opportunity to investigate the synergistic relationship of these analyt...
Source: Analytical and Bioanalytical Chemistry - July 19, 2021 Category: Chemistry Authors: G Mayne E De Bloois D Dabelea U Christians Source Type: research

Ion-exchanged glass microrods as hybrid SERS/fluorescence substrates for molecular beacon-based DNA detection
Anal Bioanal Chem. 2021 Jul 19. doi: 10.1007/s00216-021-03418-0. Online ahead of print.ABSTRACTIon-exchange in molten nitrate salts containing metal ions (i.e. silver, copper, etc.) represents a well-established technique able to modify the chemical-physical properties of glass materials. It is widely used not only in the field of integrated optics (IO) but also, more recently, in plasmonics due to the possibility to induce the formation of metal nanoparticles in the glass matrix by an ad hoc thermal post-process. In this work, the application of this technology for the realisation of low-cost and stable surface-enhanced R...
Source: Analytical and Bioanalytical Chemistry - July 19, 2021 Category: Chemistry Authors: Simone Berneschi Cristiano D'Andrea Francesco Baldini Martina Banchelli Marella de Angelis Stefano Pelli Roberto Pini Diego Pugliese Nadia G Boetti Davide Janner Daniel Milanese Ambra Giannetti Paolo Matteini Source Type: research

Development and validation of an LC-MS/MS assay for the quantification of allopregnanolone and its progesterone-derived isomers, precursors, and cortisol/cortisone in pregnancy
Anal Bioanal Chem. 2021 Jul 19. doi: 10.1007/s00216-021-03523-0. Online ahead of print.ABSTRACTNeuroactive steroids are potent neuromodulators that play a critical role in both maternal and fetal health during pregnancy. These stress-responsive compounds are reportedly low in women with perinatal depression and may be associated with poor pregnancy outcomes in animal models. Chronic stress is a risk factor for adverse birth outcomes. Simultaneous quantification of neuroactive steroids, in combination with stress hormones cortisol/cortisone, provides an opportunity to investigate the synergistic relationship of these analyt...
Source: Analytical and Bioanalytical Chemistry - July 19, 2021 Category: Chemistry Authors: G Mayne E De Bloois D Dabelea U Christians Source Type: research

Operando tracking of oxidation-state changes by coupling electrochemistry with time-resolved X-ray absorption spectroscopy demonstrated for water oxidation by a cobalt-based catalyst film
Anal Bioanal Chem. 2021 Jul 17. doi: 10.1007/s00216-021-03515-0. Online ahead of print.ABSTRACTTransition metal oxides are promising electrocatalysts for water oxidation, i.e., the oxygen evolution reaction (OER), which is critical in electrochemical production of non-fossil fuels. The involvement of oxidation state changes of the metal in OER electrocatalysis is increasingly recognized in the literature. Tracing these oxidation states under operation conditions could provide relevant information for performance optimization and development of durable catalysts, but further methodical developments are needed. Here, we prop...
Source: Analytical and Bioanalytical Chemistry - July 18, 2021 Category: Chemistry Authors: Chiara Pasquini Si Liu Petko Chernev Diego Gonzalez-Flores Mohammad Reza Mohammadi Paul Kubella Shan Jiang Stefan Loos Katharina Klingan Vadim Sikolenko Stefan Mebs Michael Haumann Paul Beyer Luca D'Amario Rodney D L Smith Ivelina Zaharieva Holger Dau Source Type: research

Parallelized label-free monitoring of cell adhesion on extracellular matrix proteins measured by single colour reflectometry
Anal Bioanal Chem. 2021 Jul 16. doi: 10.1007/s00216-021-03522-1. Online ahead of print.ABSTRACTThe understanding of the initial cell adhesion to biomaterials is crucial for the survival of implants. The manifold possibilities to tailor an implant surface and the diverse requirements for different implant applications necessitate a timesaving and highly parallelized analytical methodology. Due to its intrinsic advantages (label-free, time-resolved, robust against temperature fluctuations, and particularly the multiplexing possibilities), single colour reflectometry (SCORE) is used for the first time to investigate cell adhe...
Source: Analytical and Bioanalytical Chemistry - July 17, 2021 Category: Chemistry Authors: Johanna Hutterer G ünther Proll Peter Fechner G ünter Gauglitz Source Type: research

A fit-for-purpose copper speciation method for the determination of exchangeable copper relevant to Wilson's disease
Anal Bioanal Chem. 2021 Jul 16. doi: 10.1007/s00216-021-03517-y. Online ahead of print.ABSTRACTExchangeable copper (CuEXC), mainly comprised copper (Cu) bound to albumin, has been proposed as a specific marker of Cu overload in Wilson's disease (WD). To the author's knowledge, there are no methods capable of determining reliably CuEXC to meet the requirements and challenges faced by a clinical trial. The present work describes a novel speciation strategy for the determination of the main Cu-species in human serum by anion-exchange high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometr...
Source: Analytical and Bioanalytical Chemistry - July 17, 2021 Category: Chemistry Authors: M Estela Del Castillo Busto Susana Cuello-Nunez Christian Ward-Deitrich Tim Morley Heidi Goenaga-Infante Source Type: research

Parallelized label-free monitoring of cell adhesion on extracellular matrix proteins measured by single colour reflectometry
Anal Bioanal Chem. 2021 Jul 16. doi: 10.1007/s00216-021-03522-1. Online ahead of print.ABSTRACTThe understanding of the initial cell adhesion to biomaterials is crucial for the survival of implants. The manifold possibilities to tailor an implant surface and the diverse requirements for different implant applications necessitate a timesaving and highly parallelized analytical methodology. Due to its intrinsic advantages (label-free, time-resolved, robust against temperature fluctuations, and particularly the multiplexing possibilities), single colour reflectometry (SCORE) is used for the first time to investigate cell adhe...
Source: Analytical and Bioanalytical Chemistry - July 17, 2021 Category: Chemistry Authors: Johanna Hutterer G ünther Proll Peter Fechner G ünter Gauglitz Source Type: research

A fit-for-purpose copper speciation method for the determination of exchangeable copper relevant to Wilson's disease
Anal Bioanal Chem. 2021 Jul 16. doi: 10.1007/s00216-021-03517-y. Online ahead of print.ABSTRACTExchangeable copper (CuEXC), mainly comprised copper (Cu) bound to albumin, has been proposed as a specific marker of Cu overload in Wilson's disease (WD). To the author's knowledge, there are no methods capable of determining reliably CuEXC to meet the requirements and challenges faced by a clinical trial. The present work describes a novel speciation strategy for the determination of the main Cu-species in human serum by anion-exchange high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometr...
Source: Analytical and Bioanalytical Chemistry - July 17, 2021 Category: Chemistry Authors: M Estela Del Castillo Busto Susana Cuello-Nunez Christian Ward-Deitrich Tim Morley Heidi Goenaga-Infante Source Type: research

Genetically encoded FRET fluorescent sensor designed for detecting MOF histone acetyltransferase activity in vitro and in living cells
In this study, we reported the development of a gene-coding protein sensor. This protein sensor was designed based on the mechanism of fluorescence resonance energy transfer (FRET). The four kinds of sensors, varying from substrate and linker length, were evaluated, with ~20% increases in response efficiency. Next, sensors with different lysine mutation sites in the substrate sequence or mutation of key amino acids in the binding domain were also analyzed to determine site specificity. We found single-site lysine mutant could not cause a significant decrease in response efficiency. Furthermore, addition of MG149, a histone...
Source: Analytical and Bioanalytical Chemistry - July 16, 2021 Category: Chemistry Authors: Qianqian Han Feng Chen Shushan Liu Yushu Ge Jiang Wu Dan Liu Source Type: research

Extended sawhorse waveform for stable zinc detection with fast-scan cyclic voltammetry
Anal Bioanal Chem. 2021 Jul 16. doi: 10.1007/s00216-021-03529-8. Online ahead of print.ABSTRACTZinc (Zn(II)) is a divalent cation involved in regulating intracellular signal transduction and gene expression through transcription factor activity, and can act as a metal neurotransmitter by modulating synaptic activity and neuronal plasticity. Previous research has demonstrated spatial heterogeneity of Zn(II) in the brain, has estimated extracellular concentrations of Zn(II) across various brain regions, and has measured rapid intracellular changes in Zn(II) concentration during glutamate flux. Despite this work, quantificati...
Source: Analytical and Bioanalytical Chemistry - July 16, 2021 Category: Chemistry Authors: Anntonette N Perry Michael T Cryan Ashley E Ross Source Type: research

Correction to: An antibody-free LC-MS/MS method for the quantification of intact insulin-like growth factors 1 and 2 in human plasma
Anal Bioanal Chem. 2021 Jul 16. doi: 10.1007/s00216-021-03507-0. Online ahead of print.NO ABSTRACTPMID:34268589 | DOI:10.1007/s00216-021-03507-0 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - July 16, 2021 Category: Chemistry Authors: Mark S Pratt Martijn van Faassen Noah Remmelts Rainer Bischoff Ido P Kema Source Type: research

Genetically encoded FRET fluorescent sensor designed for detecting MOF histone acetyltransferase activity in vitro and in living cells
In this study, we reported the development of a gene-coding protein sensor. This protein sensor was designed based on the mechanism of fluorescence resonance energy transfer (FRET). The four kinds of sensors, varying from substrate and linker length, were evaluated, with ~20% increases in response efficiency. Next, sensors with different lysine mutation sites in the substrate sequence or mutation of key amino acids in the binding domain were also analyzed to determine site specificity. We found single-site lysine mutant could not cause a significant decrease in response efficiency. Furthermore, addition of MG149, a histone...
Source: Analytical and Bioanalytical Chemistry - July 16, 2021 Category: Chemistry Authors: Qianqian Han Feng Chen Shushan Liu Yushu Ge Jiang Wu Dan Liu Source Type: research

Extended sawhorse waveform for stable zinc detection with fast-scan cyclic voltammetry
Anal Bioanal Chem. 2021 Jul 16. doi: 10.1007/s00216-021-03529-8. Online ahead of print.ABSTRACTZinc (Zn(II)) is a divalent cation involved in regulating intracellular signal transduction and gene expression through transcription factor activity, and can act as a metal neurotransmitter by modulating synaptic activity and neuronal plasticity. Previous research has demonstrated spatial heterogeneity of Zn(II) in the brain, has estimated extracellular concentrations of Zn(II) across various brain regions, and has measured rapid intracellular changes in Zn(II) concentration during glutamate flux. Despite this work, quantificati...
Source: Analytical and Bioanalytical Chemistry - July 16, 2021 Category: Chemistry Authors: Anntonette N Perry Michael T Cryan Ashley E Ross Source Type: research

Correction to: An antibody-free LC-MS/MS method for the quantification of intact insulin-like growth factors 1 and 2 in human plasma
Anal Bioanal Chem. 2021 Jul 16. doi: 10.1007/s00216-021-03507-0. Online ahead of print.NO ABSTRACTPMID:34268589 | DOI:10.1007/s00216-021-03507-0 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - July 16, 2021 Category: Chemistry Authors: Mark S Pratt Martijn van Faassen Noah Remmelts Rainer Bischoff Ido P Kema Source Type: research

3D-printed microchip electrophoresis device containing spiral electrodes for integrated capacitively coupled contactless conductivity detection
Anal Bioanal Chem. 2021 Jul 14. doi: 10.1007/s00216-021-03494-2. Online ahead of print.ABSTRACTIn this work, we demonstrate for the first time the design and fabrication of microchip electrophoresis devices containing cross-shaped channels and spiral electrodes around the separation channel for microchip electrophoresis and capacitively coupled contactless conductivity detection. The whole device was prepared in a digital light processing-based 3D printer in poly(ethylene glycol) diacrylate resin. Outstanding X-Y resolution of the customized 3D printer ensured the fabrication of 40-μm cross section channels. The spiral ...
Source: Analytical and Bioanalytical Chemistry - July 15, 2021 Category: Chemistry Authors: Brenda M C Costa Aline G Coelho Michael J Beauchamp Jacob B Nielsen Gregory P Nordin Adam T Woolley Jos é A F da Silva Source Type: research

Synthesis of POMOFs with 8-fold helix and its composite with carboxyl functionalized SWCNTs for the voltammetric determination of dopamine
Anal Bioanal Chem. 2021 Jul 15. doi: 10.1007/s00216-021-03504-3. Online ahead of print.ABSTRACTAlthough many satisfactory studies have been developed for biomolecule detection, the complexity of biofluids still poses a major challenge to improve the performance of nanomaterials as electrochemical sensors. Herein, unprecedented polyoxometalate-based metal-organic frameworks (POMOFs) with 8-fold meso-helical feature, [Ag5(trz)4]2[PMo12O40] (PAZ), were synthesized and explored as electrochemical sensors to detect dopamine (DA). To improve the conductivity of PAZ and the binding ability with single-walled carbon nanotubes (SWC...
Source: Analytical and Bioanalytical Chemistry - July 15, 2021 Category: Chemistry Authors: Kunfeng Zhou Hong Han Jingquan Sha Shaozheng Luan Yan Diao Chunyao Dong Jinghua Yang Source Type: research

Non-noble metal plasmonic enhanced photoelectrochemical sensing of chlorpyrifos based on 1D TiO < sub > 2-x < /sub > /3D nitrogen-doped graphene hydrogel heterostructure
Anal Bioanal Chem. 2021 Jul 15. doi: 10.1007/s00216-021-03513-2. Online ahead of print.ABSTRACTLow-cost and resource-rich non-noble metal plasmonic materials have attracted tremendous attention as potential substitutes for plasmonic noble metals. Herein, 3D nitrogen-doped graphene hydrogels (NGH) decorated with Ti3+ self-doped 1D rod-shaped titanium dioxide nanorods (TiO2-x NR), 10-25 nm in size, were prepared by a facile one-step method. It was found that the as-fabricated TiO2-x NR/NGH showed a synergistic effect, displaying enhanced photoelectrochemical (PEC) activity by controlling the nanoscale architecture and improv...
Source: Analytical and Bioanalytical Chemistry - July 15, 2021 Category: Chemistry Authors: Xiaojiao Du Jun Sun Ding Jiang Wenhan Du Source Type: research

3D-printed microchip electrophoresis device containing spiral electrodes for integrated capacitively coupled contactless conductivity detection
Anal Bioanal Chem. 2021 Jul 14. doi: 10.1007/s00216-021-03494-2. Online ahead of print.ABSTRACTIn this work, we demonstrate for the first time the design and fabrication of microchip electrophoresis devices containing cross-shaped channels and spiral electrodes around the separation channel for microchip electrophoresis and capacitively coupled contactless conductivity detection. The whole device was prepared in a digital light processing-based 3D printer in poly(ethylene glycol) diacrylate resin. Outstanding X-Y resolution of the customized 3D printer ensured the fabrication of 40-μm cross section channels. The spiral ...
Source: Analytical and Bioanalytical Chemistry - July 15, 2021 Category: Chemistry Authors: Brenda M C Costa Aline G Coelho Michael J Beauchamp Jacob B Nielsen Gregory P Nordin Adam T Woolley Jos é A F da Silva Source Type: research

Synthesis of POMOFs with 8-fold helix and its composite with carboxyl functionalized SWCNTs for the voltammetric determination of dopamine
Anal Bioanal Chem. 2021 Jul 15. doi: 10.1007/s00216-021-03504-3. Online ahead of print.ABSTRACTAlthough many satisfactory studies have been developed for biomolecule detection, the complexity of biofluids still poses a major challenge to improve the performance of nanomaterials as electrochemical sensors. Herein, unprecedented polyoxometalate-based metal-organic frameworks (POMOFs) with 8-fold meso-helical feature, [Ag5(trz)4]2[PMo12O40] (PAZ), were synthesized and explored as electrochemical sensors to detect dopamine (DA). To improve the conductivity of PAZ and the binding ability with single-walled carbon nanotubes (SWC...
Source: Analytical and Bioanalytical Chemistry - July 15, 2021 Category: Chemistry Authors: Kunfeng Zhou Hong Han Jingquan Sha Shaozheng Luan Yan Diao Chunyao Dong Jinghua Yang Source Type: research

Non-noble metal plasmonic enhanced photoelectrochemical sensing of chlorpyrifos based on 1D TiO < sub > 2-x < /sub > /3D nitrogen-doped graphene hydrogel heterostructure
Anal Bioanal Chem. 2021 Jul 15. doi: 10.1007/s00216-021-03513-2. Online ahead of print.ABSTRACTLow-cost and resource-rich non-noble metal plasmonic materials have attracted tremendous attention as potential substitutes for plasmonic noble metals. Herein, 3D nitrogen-doped graphene hydrogels (NGH) decorated with Ti3+ self-doped 1D rod-shaped titanium dioxide nanorods (TiO2-x NR), 10-25 nm in size, were prepared by a facile one-step method. It was found that the as-fabricated TiO2-x NR/NGH showed a synergistic effect, displaying enhanced photoelectrochemical (PEC) activity by controlling the nanoscale architecture and improv...
Source: Analytical and Bioanalytical Chemistry - July 15, 2021 Category: Chemistry Authors: Xiaojiao Du Jun Sun Ding Jiang Wenhan Du Source Type: research

Simultaneous quantification of trimethylamine N-oxide, trimethylamine, choline, betaine, creatinine, and propionyl-, acetyl-, and L-carnitine in clinical and food samples using HILIC-LC-MS
Anal Bioanal Chem. 2021 Jul 13. doi: 10.1007/s00216-021-03509-y. Online ahead of print.ABSTRACTTrimethylamine-N-oxide (TMAO), a microbiome-derived metabolite from the metabolism of choline, betaine, and carnitines, is associated to adverse cardiovascular outcomes. A method suitable for routine quantification of TMAO and its precursors (trimethylamine (TMA), choline, betaine, creatinine, and propionyl-, acetyl-, and L-carnitine) in clinical and food samples has been developed based on LC-MS. TMA was successfully derivatized using iodoacetonitrile, and no cross-reactions with TMAO or the other methylamines were detected. Ext...
Source: Analytical and Bioanalytical Chemistry - July 14, 2021 Category: Chemistry Authors: Mohammed E Hefni Maria Bergstr öm Torbj örn Lennqvist Cecilia Fagerstr öm Cornelia M Witth öft Source Type: research

Discrimination of deletion to point cytokine mutants based on an array of cross-reactive receptors mimicking protein recognition by heparan sulfate
In this study, a rational biomimetic strategy has been used to prepare sensing materials capable of discriminating structurally similar proteins, such as deletion and point mutants of a cytokine, by mimicking the biological properties of heparan sulfate (HS). Using the self-assembly of two disaccharides, lactose and sulfated lactose at various ratios on the surface of a chip, an array of combinatorial cross-reactive receptors has been prepared. Coupling with surface plasmon resonance imaging (SPRi), the obtained cross-reactive array is very efficient for protein sensing. It is able to detect HS binding proteins (HSbps) suc...
Source: Analytical and Bioanalytical Chemistry - July 14, 2021 Category: Chemistry Authors: Maria Genua Laurie-Amandine Gar çon Yulia N Sergeeva Els Saesen Benjamin Musnier Arnaud Buhot Martial Billon Evelyne Gout Rabia Sadir Hugues Lortat-Jacob Christine Le Narvor David Bonnaff é Thierry Livache Yanxia Hou Source Type: research

Construction of a Tl(I) voltammetric sensor based on ZIF-67 nanocrystals: optimization of operational conditions via response surface design
Anal Bioanal Chem. 2021 Jul 14. doi: 10.1007/s00216-021-03493-3. Online ahead of print.ABSTRACTAn electroanalytical sensor was constructed constituted on a carbon paste electrode (CPE) with a ZIF-67 modifier and devoted to the quantification of Tl(I). Several characterization tests including XRD, BET, FT-IR, SEM/EDS/mapping, TEM, impedance spectroscopy (EIS), and cyclic voltammetry (CV) were performed on the synthesized ZIF-67 nanocrystals and CPE matrix. Central composite design (CCD) was used to assess the impact of variables affecting the sensor response, including the weight percent of ZIF-67 (14%), the pH of the thall...
Source: Analytical and Bioanalytical Chemistry - July 14, 2021 Category: Chemistry Authors: Farzaneh Hashemi Ali Reza Zanganeh Farid Naeimi Maryam Tayebani Source Type: research

Multiplexing neurochemical detection with carbon fiber multielectrode arrays using fast-scan cyclic voltammetry
Anal Bioanal Chem. 2021 Jul 14. doi: 10.1007/s00216-021-03526-x. Online ahead of print.ABSTRACTCarbon fiber microelectrodes (CFMEs) have been extensively used to measure neurotransmitters with fast-scan cyclic voltammetry (FSCV) due to their ability to adsorb cationic monoamine neurotransmitters. Although FSCV, in tandem with CFMEs, provides high temporal and spatial resolution, only single-channel potentiostats and electrodes have been primarily utilized. More recently, the need and use of carbon fiber multielectrode arrays has risen to target multiple brain regions. Previous studies have shown the ability to detect dopam...
Source: Analytical and Bioanalytical Chemistry - July 14, 2021 Category: Chemistry Authors: Harmain Rafi Alexander G Zestos Source Type: research

Exploiting targeted and untargeted approaches for the analysis of bacterial metabolites under altered growth conditions
Anal Bioanal Chem. 2021 Jul 12. doi: 10.1007/s00216-021-03505-2. Online ahead of print.ABSTRACTIn the host, pathogenic microorganisms have developed stress responses to cope with constantly changing environments. Stress responses are directly related to changes in several metabolomic pathways, which could hamper microorganisms' unequivocal identification. We evaluated the effect of various in vitro stress conditions (acidic, basic, oxidative, ethanolic, and saline conditions) on the metabolism of Staphylococcus aureus, Bacillus cereus, and Pseudomonas aeruginosa, which are common lung pathogens. The metabolite profiles of ...
Source: Analytical and Bioanalytical Chemistry - July 13, 2021 Category: Chemistry Authors: Delphine Zanella Tiffany Liden Jamie York Flavio A Franchina Jean-Fran çois Focant Kevin A Schug Source Type: research

A pipeline for making < sup > 31 < /sup > P NMR accessible for small- and large-scale lipidomics studies
We describe the process from sample extraction to data processing and analysis. This pipeline is important because it allows greater thoroughness in lipidomics studies and increases scope for answering scientific questions about lipid-containing systems.PMID:34254158 | DOI:10.1007/s00216-021-03430-4 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - July 13, 2021 Category: Chemistry Authors: Samuel Furse Huw E L Williams Adam J Watkins Samuel Virtue Antonio Vidal-Puig Risha Amarsi Marika Charalambous Albert Koulman Source Type: research

Correction to: Multivariate data analysis of capacitance frequency scanning for online monitoring of viable cell concentrations in small-scale bioreactors
Anal Bioanal Chem. 2021 Jul 13. doi: 10.1007/s00216-021-03508-z. Online ahead of print.NO ABSTRACTPMID:34254159 | DOI:10.1007/s00216-021-03508-z (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - July 13, 2021 Category: Chemistry Authors: Sabrina Metze Stefanie Blioch Jens Matuszczyk Gerhard Greller Christian Grimm Jochen Scholz Marek Hoehse Source Type: research

Exploiting targeted and untargeted approaches for the analysis of bacterial metabolites under altered growth conditions
Anal Bioanal Chem. 2021 Jul 12. doi: 10.1007/s00216-021-03505-2. Online ahead of print.ABSTRACTIn the host, pathogenic microorganisms have developed stress responses to cope with constantly changing environments. Stress responses are directly related to changes in several metabolomic pathways, which could hamper microorganisms' unequivocal identification. We evaluated the effect of various in vitro stress conditions (acidic, basic, oxidative, ethanolic, and saline conditions) on the metabolism of Staphylococcus aureus, Bacillus cereus, and Pseudomonas aeruginosa, which are common lung pathogens. The metabolite profiles of ...
Source: Analytical and Bioanalytical Chemistry - July 13, 2021 Category: Chemistry Authors: Delphine Zanella Tiffany Liden Jamie York Flavio A Franchina Jean-Fran çois Focant Kevin A Schug Source Type: research

A pipeline for making < sup > 31 < /sup > P NMR accessible for small- and large-scale lipidomics studies
We describe the process from sample extraction to data processing and analysis. This pipeline is important because it allows greater thoroughness in lipidomics studies and increases scope for answering scientific questions about lipid-containing systems.PMID:34254158 | DOI:10.1007/s00216-021-03430-4 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - July 13, 2021 Category: Chemistry Authors: Samuel Furse Huw E L Williams Adam J Watkins Samuel Virtue Antonio Vidal-Puig Risha Amarsi Marika Charalambous Albert Koulman Source Type: research

Correction to: Multivariate data analysis of capacitance frequency scanning for online monitoring of viable cell concentrations in small-scale bioreactors
Anal Bioanal Chem. 2021 Jul 13. doi: 10.1007/s00216-021-03508-z. Online ahead of print.NO ABSTRACTPMID:34254159 | DOI:10.1007/s00216-021-03508-z (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - July 13, 2021 Category: Chemistry Authors: Sabrina Metze Stefanie Blioch Jens Matuszczyk Gerhard Greller Christian Grimm Jochen Scholz Marek Hoehse Source Type: research

Detection of plant virus particles with a capacitive field-effect sensor
In this study, a field-effect SiO2-gate electrolyte-insulator-semiconductor (EIS) sensor was utilized for the label-free electrostatic detection of tobacco mosaic virus (TMV) particles as a model plant pathogen. The capacitive EIS sensor has been characterized regarding its TMV sensitivity by means of constant-capacitance method. The EIS sensor was able to detect biotinylated TMV particles from a solution with a TMV concentration as low as 0.025 nM. A good correlation between the registered EIS sensor signal and the density of adsorbed TMV particles assessed from scanning electron microscopy images of the SiO2-gate chip su...
Source: Analytical and Bioanalytical Chemistry - July 10, 2021 Category: Chemistry Authors: Melanie Jablonski Arshak Poghossian Michael Keusgen Christina Wege Michael J Sch öning Source Type: research

Retention dependences support highly confident identification of lipid species in human plasma by reversed-phase UHPLC/MS
Anal Bioanal Chem. 2021 Jul 9. doi: 10.1007/s00216-021-03492-4. Online ahead of print.ABSTRACTReversed-phase ultrahigh-performance liquid chromatography-mass spectrometry (RP-UHPLC/MS) method was developed with the aim to unambiguously identify a large number of lipid species from multiple lipid classes in human plasma. The optimized RP-UHPLC/MS method employed the C18 column with sub-2-μm particles with the total run time of 25 min. The chromatographic resolution was investigated with 42 standards from 18 lipid classes. The UHPLC system was coupled to high-resolution quadrupole-time-of-flight (QTOF) mass analyzer using...
Source: Analytical and Bioanalytical Chemistry - July 10, 2021 Category: Chemistry Authors: Zuzana Va ňková Ond řej Peterka Michaela Chocholou šková Denise Wolrab Robert Jir ásko Michal Hol čapek Source Type: research

Ralf Zimmermann and Luke Hanley (Eds.): Photoionization and photo-induced processes in mass spectrometry: fundamentals and applications
Anal Bioanal Chem. 2021 Jul 9. doi: 10.1007/s00216-021-03426-0. Online ahead of print.NO ABSTRACTPMID:34244836 | DOI:10.1007/s00216-021-03426-0 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - July 10, 2021 Category: Chemistry Authors: J ürgen H Gross Source Type: research

Photoelectrochemical assay for the detection of circulating tumor cells based on aptamer-Ag < sub > 2 < /sub > S nanocrystals for signal amplification
Anal Bioanal Chem. 2021 Jul 9. doi: 10.1007/s00216-021-03502-5. Online ahead of print.ABSTRACTIn this work, we developed a photoelectrochemical assay for circulating tumor cells (CTCs) detection based on hexagonal carbon-nitrogen tubes (HCNT) as visible light-sensitive materials. The MCF-7 cell was selected as the model CTC and was captured through specific recognition between epithelial cell adhesion molecules (EpCAM) on the cell surface and anti-EpCAM antibodies. Anti-EpCAM antibody-modified magnetic nanoparticles were used to enrich and separate MCF-7 cells from samples. The detection signal was amplified by Ag2S nanocr...
Source: Analytical and Bioanalytical Chemistry - July 10, 2021 Category: Chemistry Authors: Zaoxia Wang Junjun Luo Minghui Yang Xianggui Wang Source Type: research

Becoming better trainers for our learners
Anal Bioanal Chem. 2021 Jul 10. doi: 10.1007/s00216-021-03518-x. Online ahead of print.NO ABSTRACTPMID:34245325 | DOI:10.1007/s00216-021-03518-x (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - July 10, 2021 Category: Chemistry Authors: Karen Esmonde-White Source Type: research