Facile imprinted polymer for label-free highly selective potentiometric sensing of proteins: case of recombinant human erythropoietin
Anal Bioanal Chem. 2021 Apr 17. doi: 10.1007/s00216-021-03325-4. Online ahead of print.ABSTRACTIn the current study, a molecularly imprinted polymer (MIP)-based potentiometric sensor was fabricated for a label-free determination of recombinant human erythropoietin (rhEPO). The MIP sensor was operated under zero current conditions using tetra-butyl ammonium bromide as a marker ion. A highly ordered rhEPO surface imprinted layer was prepared using 3-aminopropyl triethoxysilane and tetraethoxysilane as a monomer and cross-linker, respectively, under mild reaction conditions. A two-fold increase in the signal output was obtain...
Source: Analytical and Bioanalytical Chemistry - April 18, 2021 Category: Chemistry Authors: Ahmed H Nadim May A Abd El-Aal Medhat A Al-Ghobashy Yasser S El-Saharty Source Type: research

Fungal degradation of selected medium to highly polar pesticides by Trametes versicolor: kinetics, biodegradation pathways, and ecotoxicity of treated waters
This study reports for the first time the white-rot fungus Trametes versicolor as an alternative towards the degradation of medium to highly polar pesticides such as the organophosphate malathion, and the neonicotinoids acetamiprid and imidacloprid. Specifically, T. versicolor could completely remove 1 mg/L of malathion in an Erlenmeyer flask within 48 h, while experiments of acetamiprid and imidacloprid (4 mg/L), conducted in air-pulse fluidized bioreactors, resulted in degradation percentages of 20% and 64.7%, respectively, after 7 days of operation. Enzymatic exploration studies revealed that the cytochrome P450 system,...
Source: Analytical and Bioanalytical Chemistry - April 16, 2021 Category: Chemistry Authors: Kaidi Hu Maria Vittoria Barbieri Ester L ópez-García Cristina Postigo Miren L ópez de Alda Gloria Caminal Montserrat Sarr à Source Type: research

An excellent electrochemical aptasensor for amyloid- β oligomers based on a triple-helix aptamer switch via target-triggered signal transduction DNA displacement events
Anal Bioanal Chem. 2021 Apr 16. doi: 10.1007/s00216-021-03319-2. Online ahead of print.ABSTRACTAn excellent aptasensor for electrochemical detection of amyloid-β oligomers (AβOs) at trace levels was fabricated based on a triple-helix aptamer switch (THAS) via target-triggered signal transduction DNA displacement events. Specifically, a single-stranded anti-AβO aptamer (Apt) carrying two symmetrical arm segments was first attached via Au-S binding to an Au electrode. Gold nanoparticle (GNP)-tagged signal transduction probes (GNP-STPs) were simultaneously hybridized with the two arm segments of the Apt, and a ...
Source: Analytical and Bioanalytical Chemistry - April 16, 2021 Category: Chemistry Authors: Xiaoying Wang Xuan Gu Linyu Li Bingjia Yu Liangrui Lv Qingqing Chen Mingming Xu Source Type: research

Fungal degradation of selected medium to highly polar pesticides by Trametes versicolor: kinetics, biodegradation pathways, and ecotoxicity of treated waters
This study reports for the first time the white-rot fungus Trametes versicolor as an alternative towards the degradation of medium to highly polar pesticides such as the organophosphate malathion, and the neonicotinoids acetamiprid and imidacloprid. Specifically, T. versicolor could completely remove 1 mg/L of malathion in an Erlenmeyer flask within 48 h, while experiments of acetamiprid and imidacloprid (4 mg/L), conducted in air-pulse fluidized bioreactors, resulted in degradation percentages of 20% and 64.7%, respectively, after 7 days of operation. Enzymatic exploration studies revealed that the cytochrome P450 system,...
Source: Analytical and Bioanalytical Chemistry - April 16, 2021 Category: Chemistry Authors: Kaidi Hu Maria Vittoria Barbieri Ester L ópez-García Cristina Postigo Miren L ópez de Alda Gloria Caminal Montserrat Sarr à Source Type: research

An excellent electrochemical aptasensor for amyloid- β oligomers based on a triple-helix aptamer switch via target-triggered signal transduction DNA displacement events
Anal Bioanal Chem. 2021 Apr 16. doi: 10.1007/s00216-021-03319-2. Online ahead of print.ABSTRACTAn excellent aptasensor for electrochemical detection of amyloid-β oligomers (AβOs) at trace levels was fabricated based on a triple-helix aptamer switch (THAS) via target-triggered signal transduction DNA displacement events. Specifically, a single-stranded anti-AβO aptamer (Apt) carrying two symmetrical arm segments was first attached via Au-S binding to an Au electrode. Gold nanoparticle (GNP)-tagged signal transduction probes (GNP-STPs) were simultaneously hybridized with the two arm segments of the Apt, and a ...
Source: Analytical and Bioanalytical Chemistry - April 16, 2021 Category: Chemistry Authors: Xiaoying Wang Xuan Gu Linyu Li Bingjia Yu Liangrui Lv Qingqing Chen Mingming Xu Source Type: research

Fungal degradation of selected medium to highly polar pesticides by Trametes versicolor: kinetics, biodegradation pathways, and ecotoxicity of treated waters
This study reports for the first time the white-rot fungus Trametes versicolor as an alternative towards the degradation of medium to highly polar pesticides such as the organophosphate malathion, and the neonicotinoids acetamiprid and imidacloprid. Specifically, T. versicolor could completely remove 1 mg/L of malathion in an Erlenmeyer flask within 48 h, while experiments of acetamiprid and imidacloprid (4 mg/L), conducted in air-pulse fluidized bioreactors, resulted in degradation percentages of 20% and 64.7%, respectively, after 7 days of operation. Enzymatic exploration studies revealed that the cytochrome P450 system,...
Source: Analytical and Bioanalytical Chemistry - April 16, 2021 Category: Chemistry Authors: Kaidi Hu Maria Vittoria Barbieri Ester L ópez-García Cristina Postigo Miren L ópez de Alda Gloria Caminal Montserrat Sarr à Source Type: research

An excellent electrochemical aptasensor for amyloid- β oligomers based on a triple-helix aptamer switch via target-triggered signal transduction DNA displacement events
Anal Bioanal Chem. 2021 Apr 16. doi: 10.1007/s00216-021-03319-2. Online ahead of print.ABSTRACTAn excellent aptasensor for electrochemical detection of amyloid-β oligomers (AβOs) at trace levels was fabricated based on a triple-helix aptamer switch (THAS) via target-triggered signal transduction DNA displacement events. Specifically, a single-stranded anti-AβO aptamer (Apt) carrying two symmetrical arm segments was first attached via Au-S binding to an Au electrode. Gold nanoparticle (GNP)-tagged signal transduction probes (GNP-STPs) were simultaneously hybridized with the two arm segments of the Apt, and a ...
Source: Analytical and Bioanalytical Chemistry - April 16, 2021 Category: Chemistry Authors: Xiaoying Wang Xuan Gu Linyu Li Bingjia Yu Liangrui Lv Qingqing Chen Mingming Xu Source Type: research

Development of a colorimetric sensor array based on monometallic and bimetallic nanoparticles for discrimination of triazole fungicides
Anal Bioanal Chem. 2021 Apr 14. doi: 10.1007/s00216-021-03272-0. Online ahead of print.ABSTRACTDue to the widespread use of pesticides and their harmful effects on humans and wildlife, monitoring their residual amounts in crops is critically essential but still challenging regarding the development of high-throughput approaches. Herein, a colorimetric sensor array has been proposed for discrimination and identification of triazole fungicides using monometallic and bimetallic silver and gold nanoparticles. Aggregation-induced behavior of AgNPs, AuNPs, and Au-AgNPs in the presence of four triazole fungicides produced a finge...
Source: Analytical and Bioanalytical Chemistry - April 15, 2021 Category: Chemistry Authors: Kimia Kalantari Nafiseh Fahimi-Kashani M Reza Hormozi-Nezhada Source Type: research

Publisher Correction to: 3D stochastic microsensors for molecular recognition  and determination of heregulin-α in biological samples
Anal Bioanal Chem. 2021 Apr 14. doi: 10.1007/s00216-021-03330-7. Online ahead of print.NO ABSTRACTPMID:33855604 | DOI:10.1007/s00216-021-03330-7 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 15, 2021 Category: Chemistry Authors: Raluca-Ioana Stefan-van Staden Catalina Cioates Negut Sorin Sebastian Gheorghe Alexandra Cior îță Source Type: research

Development of a colorimetric sensor array based on monometallic and bimetallic nanoparticles for discrimination of triazole fungicides
Anal Bioanal Chem. 2021 Apr 14. doi: 10.1007/s00216-021-03272-0. Online ahead of print.ABSTRACTDue to the widespread use of pesticides and their harmful effects on humans and wildlife, monitoring their residual amounts in crops is critically essential but still challenging regarding the development of high-throughput approaches. Herein, a colorimetric sensor array has been proposed for discrimination and identification of triazole fungicides using monometallic and bimetallic silver and gold nanoparticles. Aggregation-induced behavior of AgNPs, AuNPs, and Au-AgNPs in the presence of four triazole fungicides produced a finge...
Source: Analytical and Bioanalytical Chemistry - April 15, 2021 Category: Chemistry Authors: Kimia Kalantari Nafiseh Fahimi-Kashani M Reza Hormozi-Nezhada Source Type: research

Publisher Correction to: 3D stochastic microsensors for molecular recognition  and determination of heregulin-α in biological samples
Anal Bioanal Chem. 2021 Apr 14. doi: 10.1007/s00216-021-03330-7. Online ahead of print.NO ABSTRACTPMID:33855604 | DOI:10.1007/s00216-021-03330-7 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 15, 2021 Category: Chemistry Authors: Raluca-Ioana Stefan-van Staden Catalina Cioates Negut Sorin Sebastian Gheorghe Alexandra Cior îță Source Type: research

Colloidal gold-based lateral flow immunoassay with inline cleanup for rapid on-site screening of carbendazim in functional foods
In this study, for the first time, we propose a sensitive colloidal gold-based lateral flow immunoassay (LFIA) that can be used to detect carbendazim residues in functional foods. The adoption of inline cleanup LFIA strips effectively improved background interference to reduce misjudgment of results. First, the hapten 2-(methylamino)-1H-benzo[d]imidazole-5-carboxylic acid was used to establish the carbendazim immunoassay method. Subsequently, colloidal gold-mAb preparation and LFIA detection conditions were systematically optimized. For root and fruit samples (ginseng, ginger, jujube, and Chinese wolfberry), the designed s...
Source: Analytical and Bioanalytical Chemistry - April 14, 2021 Category: Chemistry Authors: Jia'an Qin Qian Lu Changjian Wang Jiaoyang Luo Meihua Yang Source Type: research

An integrated strategy of MS-network-based offline 2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP < sup > ® < /sup > -MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction
Anal Bioanal Chem. 2021 Apr 13. doi: 10.1007/s00216-021-03302-x. Online ahead of print.ABSTRACTSijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases. While there are reports on pharmaceutical substances of SJZD focusing on its polysaccharides, the composition of non-polysaccharides (NPSs) has not yet been holistically clarified. In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profilin...
Source: Analytical and Bioanalytical Chemistry - April 14, 2021 Category: Chemistry Authors: Bangjian Dong Chongsheng Peng Ping Ma Xiaobo Li Source Type: research

Mass spectrometric identification and quantification of the antibiotic clavulanic acid in broiler chicken plasma and meat as a necessary analytical tool in finding ways to increase the effectiveness of currently used antibiotics in the treatment of broiler chickens
Anal Bioanal Chem. 2021 Apr 14. doi: 10.1007/s00216-021-03307-6. Online ahead of print.ABSTRACTClavulanic acid is a molecule with antimicrobial effect used in several livestock species treatment. Its inclusion in the treatment of infectious diseases of broilers requires determination of pharmacokinetic and pharmacodynamic parameters in order to determine the appropriate dosage for broilers and ensure safety of chicken products for human health. The present study describes the optimisation of analytical LC-MS/MS method for identification and quantification of clavulanic acid in broiler chicken plasma and meat. The limit of ...
Source: Analytical and Bioanalytical Chemistry - April 14, 2021 Category: Chemistry Authors: Kristina Putecova Katerina Nedbalcova Iva Bartejsova Monika Zouharova Katarina Matiaskova Kamil Stastny Source Type: research

Antibody affinity as a driver of signal generation in a paper-based immunoassay for Ebola virus surveillance
Anal Bioanal Chem. 2021 Apr 14. doi: 10.1007/s00216-021-03317-4. Online ahead of print.ABSTRACTDuring epidemics, such as the frequent and devastating Ebola virus outbreaks that have historically plagued regions of Africa, serological surveillance efforts are critical for viral containment and the development of effective antiviral therapeutics. Antibody serology can also be used retrospectively for population-level surveillance to provide a more complete estimate of total infections. Ebola surveillance efforts rely on enzyme-linked immunosorbent assays (ELISAs), which restrict testing to laboratories and are not adaptable ...
Source: Analytical and Bioanalytical Chemistry - April 14, 2021 Category: Chemistry Authors: Lara P Murray Ramesh Govindan Andrea C Mora James B Munro Charles R Mace Source Type: research

Development of a sensitive direct injection LC-MS/MS method for the detection of glyphosate and aminomethylphosphonic acid (AMPA) in hard waters
This study represents a significant increase in sensitivity for LC-MS/MS analysis of glyphosate in hard water systems. Graphical abstract.PMID:33846826 | DOI:10.1007/s00216-021-03324-5 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 13, 2021 Category: Chemistry Authors: Jake C Ulrich P Lee Ferguson Source Type: research

Development of a sensitive direct injection LC-MS/MS method for the detection of glyphosate and aminomethylphosphonic acid (AMPA) in hard waters
This study represents a significant increase in sensitivity for LC-MS/MS analysis of glyphosate in hard water systems. Graphical abstract.PMID:33846826 | DOI:10.1007/s00216-021-03324-5 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 13, 2021 Category: Chemistry Authors: Jake C Ulrich P Lee Ferguson Source Type: research

In vivo evidence for the unique kinetics of evoked dopamine release in the patch and matrix compartments of the striatum
Anal Bioanal Chem. 2021 Apr 12. doi: 10.1007/s00216-021-03300-z. Online ahead of print.ABSTRACTThe neurochemical transmitter dopamine (DA) is implicated in a number of diseases states, including Parkinson's disease, schizophrenia, and drug abuse. DA terminal fields in the dorsal striatum and core region of the nucleus accumbens in the rat brain are organized as heterogeneous domains exhibiting fast and slow kinetic of DA release. The rates of dopamine release are significantly and substantially faster in the fast domains relative to the slow domains. The striatum is composed of a mosaic of spatial compartments known as the...
Source: Analytical and Bioanalytical Chemistry - April 12, 2021 Category: Chemistry Authors: Andrea Jaquins-Gerstl Kathryn M Nesbitt Adrian C Michael Source Type: research

In vivo evidence for the unique kinetics of evoked dopamine release in the patch and matrix compartments of the striatum
Anal Bioanal Chem. 2021 Apr 12. doi: 10.1007/s00216-021-03300-z. Online ahead of print.ABSTRACTThe neurochemical transmitter dopamine (DA) is implicated in a number of diseases states, including Parkinson's disease, schizophrenia, and drug abuse. DA terminal fields in the dorsal striatum and core region of the nucleus accumbens in the rat brain are organized as heterogeneous domains exhibiting fast and slow kinetic of DA release. The rates of dopamine release are significantly and substantially faster in the fast domains relative to the slow domains. The striatum is composed of a mosaic of spatial compartments known as the...
Source: Analytical and Bioanalytical Chemistry - April 12, 2021 Category: Chemistry Authors: Andrea Jaquins-Gerstl Kathryn M Nesbitt Adrian C Michael Source Type: research

Nitrogen monoxide as dopant for enhanced selectivity of isomeric monoterpenes in drift tube ion mobility spectrometry with < sup > 3 < /sup > H ionization
Anal Bioanal Chem. 2021 Apr 10. doi: 10.1007/s00216-021-03306-7. Online ahead of print.ABSTRACTThe ion mobility spectra of the isomeric monoterpenes α-pinene, β-pinene, myrcene, and limonene in drift tube ion mobility spectrometry (IMS) with 3H radioactive ionization are highly similar and difficult to distinguish. The aim of this work was to enhance the selectivity of IMS by the addition of nitrogen monoxide (NO) as dopant and to investigate the underlying changes in ion formation responsible for the modified ion signals observed in the ion mobility spectra. Even though 3H-based-IMS systems have been used in hy...
Source: Analytical and Bioanalytical Chemistry - April 11, 2021 Category: Chemistry Authors: Rebecca Brendel Sascha Rohn Philipp Weller Source Type: research

Remote pandemic teaching in quantitative and instrumental chemical analysis courses at a Hispanic serving institution
Anal Bioanal Chem. 2021 Apr 11. doi: 10.1007/s00216-021-03243-5. Online ahead of print.NO ABSTRACTPMID:33839917 | DOI:10.1007/s00216-021-03243-5 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 11, 2021 Category: Chemistry Authors: Ángel L Morales-Cruz Bonny M Ortiz-Andrade Joselyn Del Pilar-Albaladejo Liz M D íaz-Vázquez Uriel Rivera-Gonz ález Vilmal í López-Mejías Source Type: research

Characterization and purification of pentameric chimeric protein particles using asymmetric flow field-flow fractionation coupled with multiple detectors
Anal Bioanal Chem. 2021 Apr 10. doi: 10.1007/s00216-021-03323-6. Online ahead of print.ABSTRACTPorcine circovirus causes the post-weaning multi-systemic wasting syndrome. Despite the existence of commercial vaccines, the development of more effective and cheaper vaccines is expected. The usage of chimeric antigens allows serological differentiation between naturally infected and vaccinated animals. In this work, recombinant pentameric vaccination protein particles spontaneously assembled from identical subunits-chimeric fusion proteins derived from circovirus capsid antigen Cap and a multimerizing subunit of mouse polyomav...
Source: Analytical and Bioanalytical Chemistry - April 10, 2021 Category: Chemistry Authors: Jan Kotoucek Renata Hezova Alena Vrablikova Frantisek Hubatka Pavel Kulich Stuart Macaulay Dierk Roessner Milan Raska Ivan Psikal Jaroslav Turanek Source Type: research

Characterization and purification of pentameric chimeric protein particles using asymmetric flow field-flow fractionation coupled with multiple detectors
Anal Bioanal Chem. 2021 Apr 10. doi: 10.1007/s00216-021-03323-6. Online ahead of print.ABSTRACTPorcine circovirus causes the post-weaning multi-systemic wasting syndrome. Despite the existence of commercial vaccines, the development of more effective and cheaper vaccines is expected. The usage of chimeric antigens allows serological differentiation between naturally infected and vaccinated animals. In this work, recombinant pentameric vaccination protein particles spontaneously assembled from identical subunits-chimeric fusion proteins derived from circovirus capsid antigen Cap and a multimerizing subunit of mouse polyomav...
Source: Analytical and Bioanalytical Chemistry - April 10, 2021 Category: Chemistry Authors: Jan Kotoucek Renata Hezova Alena Vrablikova Frantisek Hubatka Pavel Kulich Stuart Macaulay Dierk Roessner Milan Raska Ivan Psikal Jaroslav Turanek Source Type: research

Rolling circle amplification with fluorescently labeled dUTP-balancing the yield and degree of labeling
Anal Bioanal Chem. 2021 Apr 8. doi: 10.1007/s00216-021-03322-7. Online ahead of print.ABSTRACTDetection methods based on rolling circle amplification (RCA) have been applied to a large number of targets in molecular biology. The key feature of RCA-based methods as well as other nucleic acid amplification methods is their exceptional sensitivity, which allows the detection of molecules at low concentrations, achieved by signal amplification due to nucleic acid magnification and subsequent detection. Variations on the method, such as immuno-RCA, extend the range of potential targets that can be detected. Employing fluorescen...
Source: Analytical and Bioanalytical Chemistry - April 9, 2021 Category: Chemistry Authors: M S Goryunova V K Arzhanik S K Zavriev D Y Ryazantsev Source Type: research

A portable instrument for on-site detection of heavy metal ions in water
Anal Bioanal Chem. 2021 Apr 9. doi: 10.1007/s00216-021-03292-w. Online ahead of print.ABSTRACTBased on differential pulse voltammetry technology, we developed a portable and affordable instrument for on-site detection of trace heavy metal pollutants in liquid through a disposable plastic pipette. It mainly consists of a six-electrode electrochemical sensor which is integrated in the instrument. The pipette chip is equipped with a pump valve, and thus, it can avoid contamination. We have analyzed the sensitivity and specificity of the electrochemical sensor for heavy metal detection. Experimental results demonstrated that t...
Source: Analytical and Bioanalytical Chemistry - April 9, 2021 Category: Chemistry Authors: Zhenqing Li Debao Xu Dawei Zhang Yoshinori Yamaguchi Source Type: research

Rolling circle amplification with fluorescently labeled dUTP-balancing the yield and degree of labeling
Anal Bioanal Chem. 2021 Apr 8. doi: 10.1007/s00216-021-03322-7. Online ahead of print.ABSTRACTDetection methods based on rolling circle amplification (RCA) have been applied to a large number of targets in molecular biology. The key feature of RCA-based methods as well as other nucleic acid amplification methods is their exceptional sensitivity, which allows the detection of molecules at low concentrations, achieved by signal amplification due to nucleic acid magnification and subsequent detection. Variations on the method, such as immuno-RCA, extend the range of potential targets that can be detected. Employing fluorescen...
Source: Analytical and Bioanalytical Chemistry - April 9, 2021 Category: Chemistry Authors: M S Goryunova V K Arzhanik S K Zavriev D Y Ryazantsev Source Type: research

A portable instrument for on-site detection of heavy metal ions in water
Anal Bioanal Chem. 2021 Apr 9. doi: 10.1007/s00216-021-03292-w. Online ahead of print.ABSTRACTBased on differential pulse voltammetry technology, we developed a portable and affordable instrument for on-site detection of trace heavy metal pollutants in liquid through a disposable plastic pipette. It mainly consists of a six-electrode electrochemical sensor which is integrated in the instrument. The pipette chip is equipped with a pump valve, and thus, it can avoid contamination. We have analyzed the sensitivity and specificity of the electrochemical sensor for heavy metal detection. Experimental results demonstrated that t...
Source: Analytical and Bioanalytical Chemistry - April 9, 2021 Category: Chemistry Authors: Zhenqing Li Debao Xu Dawei Zhang Yoshinori Yamaguchi Source Type: research

Photothermometric analysis of bismuth ions using aggregation-induced nanozyme system with a target-triggered surface cleaning effect
This study not only provides a more accurate understanding of the aggregation-induced nanozymes based on the surface cleaning principle, but also shows the potential applications of aggregation-induced nanozymes in the POCT field.PMID:33829276 | DOI:10.1007/s00216-021-03312-9 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 8, 2021 Category: Chemistry Authors: Kehui Zhang Xibin Zhou Xin Xue Mingyue Luo Xiuhui Liu Zhonghua Xue Source Type: research

Metabotype analysis of Mthfd1l-null mouse embryos using desorption electrospray ionization mass spectrometry imaging
Anal Bioanal Chem. 2021 Apr 8. doi: 10.1007/s00216-021-03308-5. Online ahead of print.ABSTRACTMammalian folate-dependent one-carbon (1C) metabolism provides the building blocks essential during development via amino acid interconversion, methyl-donor production, regeneration of redox factors, and de novo purine and thymidylate synthesis. Folate supplementation prevents many neural tube defects (NTDs) that occur during the embryonic process of neurulation. The mechanism by which folate functions during neurulation is not well understood, and not all NTDs are preventable by folate supplementation. Mthfd1l is a mitochondrial ...
Source: Analytical and Bioanalytical Chemistry - April 8, 2021 Category: Chemistry Authors: Amanda Vaughn Rachel J DeHoog Livia S Eberlin Dean R Appling Source Type: research

TiO < sub > 2 < /sub > Nano-test tubes as a solid visual platform for sensitive Pb < sup > 2+ < /sup > ion detection based on a fluorescence resonance energy transfer (FRET) process
Anal Bioanal Chem. 2021 Apr 6. doi: 10.1007/s00216-021-03310-x. Online ahead of print.ABSTRACTA cost-effective, facile, and sensitive fluorescence sensing strategy for Pb2+ ion detection has been developed based on the fluorescence resonance energy transfer (FRET) between carbon quantum dots (CQDs) and Au nanoparticles (NPs). Glutathione (GSH)-synthesized CQDs acted as both the fluorescence donor and the sorbent to extract Pb2+ ions from the solution via Pb-GSH complexes. Pb2+ ions on CQDs reacted with -SH groups on AuNPs to generate sandwich-type Au-PdS-CQDs, leading to a dramatic decrease in the fluorescence of the CQDs....
Source: Analytical and Bioanalytical Chemistry - April 7, 2021 Category: Chemistry Authors: Ya-Hang Li Chen-Xi Zhao Yang Li Zhida Gao Xi Zhang Yan-Yan Song Source Type: research

Novel nucleic acid origami structures and conventional molecular beacon-based platforms: a comparison in biosensing applications
We present nucleic acid scaffolds divided into conventional hairpin molecular beacons and DNA origami, and discuss some relevant examples by focusing on peculiar aspects exploited in biosensing applications. We also critically evaluate analytical uses of the synthetic nucleic acid structures in biosensing to point out similarities and differences between traditional hairpin nucleic acid sequences and DNA origami.PMID:33825006 | DOI:10.1007/s00216-021-03309-4 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 7, 2021 Category: Chemistry Authors: Noemi Bellassai Roberta D'Agata Giuseppe Spoto Source Type: research

Rapid, simultaneous detection of mycotoxins with smartphone recognition-based immune microspheres
This study provides a universal rapid detection method for on-site application of large-scale food security testing. Schematic diagram of the construction of the smartphone detection system: The system is divided into three parts: detection, image capture and analysis, and result.PMID:33825917 | DOI:10.1007/s00216-021-03316-5 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 7, 2021 Category: Chemistry Authors: Lili Zhang Ziyu Zhang Yu Tian Meihui Cui Beibei Huang Tao Luo Shufang Zhang Hanjie Wang Source Type: research

TiO < sub > 2 < /sub > Nano-test tubes as a solid visual platform for sensitive Pb < sup > 2+ < /sup > ion detection based on a fluorescence resonance energy transfer (FRET) process
Anal Bioanal Chem. 2021 Apr 6. doi: 10.1007/s00216-021-03310-x. Online ahead of print.ABSTRACTA cost-effective, facile, and sensitive fluorescence sensing strategy for Pb2+ ion detection has been developed based on the fluorescence resonance energy transfer (FRET) between carbon quantum dots (CQDs) and Au nanoparticles (NPs). Glutathione (GSH)-synthesized CQDs acted as both the fluorescence donor and the sorbent to extract Pb2+ ions from the solution via Pb-GSH complexes. Pb2+ ions on CQDs reacted with -SH groups on AuNPs to generate sandwich-type Au-PdS-CQDs, leading to a dramatic decrease in the fluorescence of the CQDs....
Source: Analytical and Bioanalytical Chemistry - April 7, 2021 Category: Chemistry Authors: Ya-Hang Li Chen-Xi Zhao Yang Li Zhida Gao Xi Zhang Yan-Yan Song Source Type: research

Novel nucleic acid origami structures and conventional molecular beacon-based platforms: a comparison in biosensing applications
We present nucleic acid scaffolds divided into conventional hairpin molecular beacons and DNA origami, and discuss some relevant examples by focusing on peculiar aspects exploited in biosensing applications. We also critically evaluate analytical uses of the synthetic nucleic acid structures in biosensing to point out similarities and differences between traditional hairpin nucleic acid sequences and DNA origami.PMID:33825006 | DOI:10.1007/s00216-021-03309-4 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 7, 2021 Category: Chemistry Authors: Noemi Bellassai Roberta D'Agata Giuseppe Spoto Source Type: research

Rapid, simultaneous detection of mycotoxins with smartphone recognition-based immune microspheres
This study provides a universal rapid detection method for on-site application of large-scale food security testing. Schematic diagram of the construction of the smartphone detection system: The system is divided into three parts: detection, image capture and analysis, and result.PMID:33825917 | DOI:10.1007/s00216-021-03316-5 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 7, 2021 Category: Chemistry Authors: Lili Zhang Ziyu Zhang Yu Tian Meihui Cui Beibei Huang Tao Luo Shufang Zhang Hanjie Wang Source Type: research

A strategy combining solid-phase extraction, multiple mass defect filtering and molecular networking for rapid structural classification and annotation of natural products: characterization of chemical diversity in Citrus aurantium as a case study
Anal Bioanal Chem. 2021 Apr 6. doi: 10.1007/s00216-021-03201-1. Online ahead of print.ABSTRACTMedicinal plants are complex chemical systems containing thousands of secondary metabolites. The rapid classification and characterization of the components in medicinal plants using mass spectrometry (MS) remains an immense challenge. Herein, a novel strategy is presented for MS through the combination of solid-phase extraction (SPE), multiple mass defect filtering (MMDF) and molecular networking (MN). This strategy enables efficient classification and annotation of natural products. When combined with SPE and MMDF, the improved ...
Source: Analytical and Bioanalytical Chemistry - April 6, 2021 Category: Chemistry Authors: Yi-Kun Wang Xue-Rong Xiao Zi-Meng Zhou Yao Xiao Wei-Feng Zhu Hong-Ning Liu Fei Li Source Type: research

A strategy combining solid-phase extraction, multiple mass defect filtering and molecular networking for rapid structural classification and annotation of natural products: characterization of chemical diversity in Citrus aurantium as a case study
Anal Bioanal Chem. 2021 Apr 6. doi: 10.1007/s00216-021-03201-1. Online ahead of print.ABSTRACTMedicinal plants are complex chemical systems containing thousands of secondary metabolites. The rapid classification and characterization of the components in medicinal plants using mass spectrometry (MS) remains an immense challenge. Herein, a novel strategy is presented for MS through the combination of solid-phase extraction (SPE), multiple mass defect filtering (MMDF) and molecular networking (MN). This strategy enables efficient classification and annotation of natural products. When combined with SPE and MMDF, the improved ...
Source: Analytical and Bioanalytical Chemistry - April 6, 2021 Category: Chemistry Authors: Yi-Kun Wang Xue-Rong Xiao Zi-Meng Zhou Yao Xiao Wei-Feng Zhu Hong-Ning Liu Fei Li Source Type: research

Analysis of 14 endocannabinoids and endocannabinoid congeners in human plasma using column switching high-performance atmospheric pressure chemical ionization liquid chromatography-mass spectrometry
Anal Bioanal Chem. 2021 Apr 5. doi: 10.1007/s00216-021-03280-0. Online ahead of print.ABSTRACTThe endocannabinoid system (ECS) is a complex cell-signaling system. To address the growing need of analytics capturing endocannabinoid levels to investigate the ECS, we developed and validated an assay for the quantitative analysis of 14 endocannabinoids and congeners. A simple extraction using protein precipitation with acetonitrile followed by online-trapping high-performance liquid chromatography-tandem mass spectrometry (LC/LC-MS/MS) was used to monitor the levels of 14 endocannabinoids in plasma. The assay was validated and ...
Source: Analytical and Bioanalytical Chemistry - April 5, 2021 Category: Chemistry Authors: Cristina Sempio Jelena Klawitter Matthew Jackson Francesca Freni Ryan Shillingburg Kent Hutchison L Cinnamon Bidwell Uwe Christians Jost Klawitter Source Type: research

Polyethyleneimine-functionalized Fe < sub > 3 < /sub > O < sub > 4 < /sub > /attapulgite particles for hydrophilic interaction-based magnetic dispersive solid-phase extraction of fluoroquinolones in chicken muscle
In this study, novel polyethyleneimine-functionalized Fe3O4/attapulgite magnetic particles were prepared by a simple co-mixing method and applied as hydrophilic sorbents for the magnetic dispersive solid-phase extraction (MSPE) of three FQs, i.e., ciprofloxacin, norfloxacin, and enrofloxacin, from chicken muscle samples. The preparation of the magnetic particles was of high reproducibility and the products could be reused many times with high adsorption capacity. The key experimental factors possibly influencing the extraction efficiencies, including sample solution, extraction time, sample loading volume, desorption solut...
Source: Analytical and Bioanalytical Chemistry - April 4, 2021 Category: Chemistry Authors: Xiangdong Li Yihui Chen Shubing Chen Chunyan Hou Rongrong Xuan Yajie Gao Shuaijun Ren Lihui Yao Tingting Wang Lihua Zhang Yukui Zhang Source Type: research

Polyethyleneimine-functionalized Fe < sub > 3 < /sub > O < sub > 4 < /sub > /attapulgite particles for hydrophilic interaction-based magnetic dispersive solid-phase extraction of fluoroquinolones in chicken muscle
In this study, novel polyethyleneimine-functionalized Fe3O4/attapulgite magnetic particles were prepared by a simple co-mixing method and applied as hydrophilic sorbents for the magnetic dispersive solid-phase extraction (MSPE) of three FQs, i.e., ciprofloxacin, norfloxacin, and enrofloxacin, from chicken muscle samples. The preparation of the magnetic particles was of high reproducibility and the products could be reused many times with high adsorption capacity. The key experimental factors possibly influencing the extraction efficiencies, including sample solution, extraction time, sample loading volume, desorption solut...
Source: Analytical and Bioanalytical Chemistry - April 4, 2021 Category: Chemistry Authors: Xiangdong Li Yihui Chen Shubing Chen Chunyan Hou Rongrong Xuan Yajie Gao Shuaijun Ren Lihui Yao Tingting Wang Lihua Zhang Yukui Zhang Source Type: research

Drop-of-blood acoustic tweezing technique for integrative turbidimetric and elastometric measurement of blood coagulation
This study introduces a containerless drop-of-blood method for coagulation analysis, termed "integrated quasi-static acoustic tweezing thromboelastometry" (i-QATT™), that addresses these needs. In i-QATT™, a single drop of blood is forced to levitate and deform by the acoustic radiation force. Coagulation-induced changes in drop turbidity and firmness are measured simultaneously at different instants. The parameters describing early, intermediate, and late stages of the coagulation process are evaluated from the resulting graphical outputs. i-QATT™ rapidly ( 0.9) was established between fibrinog...
Source: Analytical and Bioanalytical Chemistry - April 2, 2021 Category: Chemistry Authors: Daishen Luo Erika M Chelales Millicent M Beard Nithya Kasireddy Damir B Khismatullin Source Type: research

Smartphone-enabled field monitoring tool for rapid hexavalent chromium detection in water
Anal Bioanal Chem. 2021 Apr 2. doi: 10.1007/s00216-021-03291-x. Online ahead of print.ABSTRACTChromium contamination of soil and water is a serious environmental and public health concern as the hexavalent form of chromium [Cr(VI)] is readily soluble in water and is a confirmed carcinogen. There is an imminent need for a robust, low-cost, and simple analytical technique to facilitate in situ monitoring of Cr(VI) in water. Current quantitative methods of Cr(VI) detection are largely laboratory-based, time-consuming, expensive, and require training for implementation. In this contribution, a portable, easy-to-use, and compac...
Source: Analytical and Bioanalytical Chemistry - April 2, 2021 Category: Chemistry Authors: Sushant D Bamane Vinod Bhojwani Pradeep L Balkunde Mainak Bhattacharya Ishan Gupta Ashwini K Mohapatra Aditya Shekhar Abhas Singh Source Type: research

Prognosis prediction of hepatocellular carcinoma after surgical resection based on serum metabolic profiling from gas chromatography-mass spectrometry
This study demonstrated that metabolomics is a powerful tool for risk screening of HCC prognosis.PMID:33796932 | DOI:10.1007/s00216-021-03281-z (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 2, 2021 Category: Chemistry Authors: Chengnan Fang Benzhe Su Tianyi Jiang Chao Li Yexiong Tan Qingqing Wang Liwei Dong Xinyu Liu Xiaohui Lin Guowang Xu Source Type: research

Investigation of lipase-ligand interactions in porcine pancreatic extracts by microscale thermophoresis
Anal Bioanal Chem. 2021 Apr 2. doi: 10.1007/s00216-021-03314-7. Online ahead of print.ABSTRACTThe evaluation of binding affinities between large biomolecules and small ligands is challenging and requires highly sensitive techniques. Microscale thermophoresis (MST) is an emerging biophysical technique used to overcome this limitation. This work describes the first MST binding method to evaluate binding affinities of small ligands to lipases from crude porcine pancreatic extracts. The conditions of the MST assay were thoroughly optimized to successfully evaluate the dissociation constant (Kd) between pancreatic lipases (PL) ...
Source: Analytical and Bioanalytical Chemistry - April 2, 2021 Category: Chemistry Authors: Ghassan Al Hamoui Dit Banni Rouba Nasreddine Syntia Fayad Cyril Colas Axel Marchal Reine Nehm é Source Type: research

Drop-of-blood acoustic tweezing technique for integrative turbidimetric and elastometric measurement of blood coagulation
This study introduces a containerless drop-of-blood method for coagulation analysis, termed "integrated quasi-static acoustic tweezing thromboelastometry" (i-QATT™), that addresses these needs. In i-QATT™, a single drop of blood is forced to levitate and deform by the acoustic radiation force. Coagulation-induced changes in drop turbidity and firmness are measured simultaneously at different instants. The parameters describing early, intermediate, and late stages of the coagulation process are evaluated from the resulting graphical outputs. i-QATT™ rapidly ( 0.9) was established between fibrinog...
Source: Analytical and Bioanalytical Chemistry - April 2, 2021 Category: Chemistry Authors: Daishen Luo Erika M Chelales Millicent M Beard Nithya Kasireddy Damir B Khismatullin Source Type: research

Smartphone-enabled field monitoring tool for rapid hexavalent chromium detection in water
Anal Bioanal Chem. 2021 Apr 2. doi: 10.1007/s00216-021-03291-x. Online ahead of print.ABSTRACTChromium contamination of soil and water is a serious environmental and public health concern as the hexavalent form of chromium [Cr(VI)] is readily soluble in water and is a confirmed carcinogen. There is an imminent need for a robust, low-cost, and simple analytical technique to facilitate in situ monitoring of Cr(VI) in water. Current quantitative methods of Cr(VI) detection are largely laboratory-based, time-consuming, expensive, and require training for implementation. In this contribution, a portable, easy-to-use, and compac...
Source: Analytical and Bioanalytical Chemistry - April 2, 2021 Category: Chemistry Authors: Sushant D Bamane Vinod Bhojwani Pradeep L Balkunde Mainak Bhattacharya Ishan Gupta Ashwini K Mohapatra Aditya Shekhar Abhas Singh Source Type: research

Prognosis prediction of hepatocellular carcinoma after surgical resection based on serum metabolic profiling from gas chromatography-mass spectrometry
This study demonstrated that metabolomics is a powerful tool for risk screening of HCC prognosis.PMID:33796932 | DOI:10.1007/s00216-021-03281-z (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 2, 2021 Category: Chemistry Authors: Chengnan Fang Benzhe Su Tianyi Jiang Chao Li Yexiong Tan Qingqing Wang Liwei Dong Xinyu Liu Xiaohui Lin Guowang Xu Source Type: research

Investigation of lipase-ligand interactions in porcine pancreatic extracts by microscale thermophoresis
Anal Bioanal Chem. 2021 Apr 2. doi: 10.1007/s00216-021-03314-7. Online ahead of print.ABSTRACTThe evaluation of binding affinities between large biomolecules and small ligands is challenging and requires highly sensitive techniques. Microscale thermophoresis (MST) is an emerging biophysical technique used to overcome this limitation. This work describes the first MST binding method to evaluate binding affinities of small ligands to lipases from crude porcine pancreatic extracts. The conditions of the MST assay were thoroughly optimized to successfully evaluate the dissociation constant (Kd) between pancreatic lipases (PL) ...
Source: Analytical and Bioanalytical Chemistry - April 2, 2021 Category: Chemistry Authors: Ghassan Al Hamoui Dit Banni Rouba Nasreddine Syntia Fayad Cyril Colas Axel Marchal Reine Nehm é Source Type: research

Drop-of-blood acoustic tweezing technique for integrative turbidimetric and elastometric measurement of blood coagulation
This study introduces a containerless drop-of-blood method for coagulation analysis, termed "integrated quasi-static acoustic tweezing thromboelastometry" (i-QATT™), that addresses these needs. In i-QATT™, a single drop of blood is forced to levitate and deform by the acoustic radiation force. Coagulation-induced changes in drop turbidity and firmness are measured simultaneously at different instants. The parameters describing early, intermediate, and late stages of the coagulation process are evaluated from the resulting graphical outputs. i-QATT™ rapidly ( 0.9) was established between fibrinog...
Source: Analytical and Bioanalytical Chemistry - April 2, 2021 Category: Chemistry Authors: Daishen Luo Erika M Chelales Millicent M Beard Nithya Kasireddy Damir B Khismatullin Source Type: research

Smartphone-enabled field monitoring tool for rapid hexavalent chromium detection in water
Anal Bioanal Chem. 2021 Apr 2. doi: 10.1007/s00216-021-03291-x. Online ahead of print.ABSTRACTChromium contamination of soil and water is a serious environmental and public health concern as the hexavalent form of chromium [Cr(VI)] is readily soluble in water and is a confirmed carcinogen. There is an imminent need for a robust, low-cost, and simple analytical technique to facilitate in situ monitoring of Cr(VI) in water. Current quantitative methods of Cr(VI) detection are largely laboratory-based, time-consuming, expensive, and require training for implementation. In this contribution, a portable, easy-to-use, and compac...
Source: Analytical and Bioanalytical Chemistry - April 2, 2021 Category: Chemistry Authors: Sushant D Bamane Vinod Bhojwani Pradeep L Balkunde Mainak Bhattacharya Ishan Gupta Ashwini K Mohapatra Aditya Shekhar Abhas Singh Source Type: research