Metabolic Recoding of NSUN2-Mediated m < sup > 5 < /sup > C Modification Promotes the Progression of Colorectal Cancer via the NSUN2/YBX1/m < sup > 5 < /sup > C-ENO1 Positive Feedback Loop
Adv Sci (Weinh). 2024 May 20:e2309840. doi: 10.1002/advs.202309840. Online ahead of print.ABSTRACTThe RNA modification, 5-methylcytosine (m5C), has recently gained prominence as a pivotal post-transcriptional regulator of gene expression, intricately intertwined with various tumorigenic processes. However, the exact mechanisms governing m5C modifications during the onset and progression of colorectal cancer (CRC) remain unclear. Here, it is determined that the m5C methyltransferase NSUN2 exhibits significantly elevated expression and exerts an oncogenic function in CRC. Mechanistically, NSUN2 and YBX1 are identified as the...
Source: Anal Sci - May 21, 2024 Category: Chemistry Authors: Baoxiang Chen Yanrong Deng Yuntian Hong Lifang Fan Xiang Zhai Heng Hu Siyuan Yin Quanjiao Chen Xiaoyu Xie Xianghai Ren Jianhong Zhao Congqing Jiang Source Type: research

Optical lateral flow assays in early diagnosis of SARS-CoV-2 infection
Anal Sci. 2024 May 17. doi: 10.1007/s44211-024-00596-6. Online ahead of print.ABSTRACTSo far, the 2019 novel coronavirus (COVID-19) is spreading widely worldwide. The early diagnosis of infection by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is essential to provide timely treatment and prevent its further spread. Lateral flow assays (LFAs) have the advantages of rapid detection, simple operation, low cost, ease of mass production, and no need for special devices and professional operators, which make them suitable for self-testing at home. This review focuses on the early diagnosis of SARS-CoV-2 infec...
Source: Anal Sci - May 17, 2024 Category: Chemistry Authors: Rushi Liang Aiping Fan Feiqian Wang Yajing Niu Source Type: research

Optical lateral flow assays in early diagnosis of SARS-CoV-2 infection
Anal Sci. 2024 May 17. doi: 10.1007/s44211-024-00596-6. Online ahead of print.ABSTRACTSo far, the 2019 novel coronavirus (COVID-19) is spreading widely worldwide. The early diagnosis of infection by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is essential to provide timely treatment and prevent its further spread. Lateral flow assays (LFAs) have the advantages of rapid detection, simple operation, low cost, ease of mass production, and no need for special devices and professional operators, which make them suitable for self-testing at home. This review focuses on the early diagnosis of SARS-CoV-2 infec...
Source: Anal Sci - May 17, 2024 Category: Chemistry Authors: Rushi Liang Aiping Fan Feiqian Wang Yajing Niu Source Type: research

Optical lateral flow assays in early diagnosis of SARS-CoV-2 infection
Anal Sci. 2024 May 17. doi: 10.1007/s44211-024-00596-6. Online ahead of print.ABSTRACTSo far, the 2019 novel coronavirus (COVID-19) is spreading widely worldwide. The early diagnosis of infection by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is essential to provide timely treatment and prevent its further spread. Lateral flow assays (LFAs) have the advantages of rapid detection, simple operation, low cost, ease of mass production, and no need for special devices and professional operators, which make them suitable for self-testing at home. This review focuses on the early diagnosis of SARS-CoV-2 infec...
Source: Anal Sci - May 17, 2024 Category: Chemistry Authors: Rushi Liang Aiping Fan Feiqian Wang Yajing Niu Source Type: research

Molecular epidemiological investigation and recombination analysis of Cachavirus prevalent in China
This study may be beneficial for evaluating the prevalence of CachaVs in China, thereby understanding the evolution trend of CachaVs.PMID:38751804 | PMC:PMC11094709 | DOI:10.3389/fvets.2024.1375948 (Source: Anal Sci)
Source: Anal Sci - May 16, 2024 Category: Chemistry Authors: Chaoliang Leng Xiang Tian Hongyue Zhai Jun Ji Lunguang Yao Source Type: research

Comprehensive extraction study using N,N-dioctylthiodiglycolamic acid: effect of S donor on metal extraction
Anal Sci. 2024 May 16. doi: 10.1007/s44211-024-00577-9. Online ahead of print.ABSTRACTExtraction ability of N,N-dioctylthiodiglycolamic acid (T-DODGAA), a soft-base sulfur donor ligand with an amide group and a carboxylic acid connected by a thioether chain, for 56 metal ions have been comprehensively investigated and compared with that of N,N-dioctyldiglycolamic acid (DODGAA) with an etheric oxygen atom, a hard-base donor. The acid dissociation constant (pKa) of the thiodiglycolamic acid framework was determined to be 3.71 ± 0.06 in water (0.1 M LiCl, 25 °C) by potentiometric titration, indicating that T-DODGAA is a sli...
Source: Anal Sci - May 16, 2024 Category: Chemistry Authors: Kojiro Shimojo Iori Fujiwara Takumi Saito Tatsuya Oshima Source Type: research

Application of on-line sample preconcentration by large-volume dual preconcentration by isotachophoresis and stacking (LDIS) on straight-channel microchips
In this study, large-volume dual preconcentration by isotachophoresis and stacking (LDIS) which is an on-line sample preconcentration technique coupling large-volume sample stacking with an electroosmotic flow pump (LVSEP) with transient isotachophoresis (tITP) was applied to microchip electrophoresis (MCE) for improving both detection sensitivities and peak shapes. To realize LDIS in MCE, we investigated experimental procedures for injecting a short plug of a leading electrolyte (LE) solution into a straight microchannel without any sophisticated injector apparatus. We found that a short LE plug could be injected into a s...
Source: Anal Sci - May 16, 2024 Category: Chemistry Authors: Fumihiko Kitagawa Kazuki Takahashi Reina Osanai Ryota Sasaki Takayuki Kawai Source Type: research

Molecular epidemiological investigation and recombination analysis of Cachavirus prevalent in China
This study may be beneficial for evaluating the prevalence of CachaVs in China, thereby understanding the evolution trend of CachaVs.PMID:38751804 | PMC:PMC11094709 | DOI:10.3389/fvets.2024.1375948 (Source: Anal Sci)
Source: Anal Sci - May 16, 2024 Category: Chemistry Authors: Chaoliang Leng Xiang Tian Hongyue Zhai Jun Ji Lunguang Yao Source Type: research

Comprehensive extraction study using N,N-dioctylthiodiglycolamic acid: effect of S donor on metal extraction
Anal Sci. 2024 May 16. doi: 10.1007/s44211-024-00577-9. Online ahead of print.ABSTRACTExtraction ability of N,N-dioctylthiodiglycolamic acid (T-DODGAA), a soft-base sulfur donor ligand with an amide group and a carboxylic acid connected by a thioether chain, for 56 metal ions have been comprehensively investigated and compared with that of N,N-dioctyldiglycolamic acid (DODGAA) with an etheric oxygen atom, a hard-base donor. The acid dissociation constant (pKa) of the thiodiglycolamic acid framework was determined to be 3.71 ± 0.06 in water (0.1 M LiCl, 25 °C) by potentiometric titration, indicating that T-DODGAA is a sli...
Source: Anal Sci - May 16, 2024 Category: Chemistry Authors: Kojiro Shimojo Iori Fujiwara Takumi Saito Tatsuya Oshima Source Type: research

Application of on-line sample preconcentration by large-volume dual preconcentration by isotachophoresis and stacking (LDIS) on straight-channel microchips
In this study, large-volume dual preconcentration by isotachophoresis and stacking (LDIS) which is an on-line sample preconcentration technique coupling large-volume sample stacking with an electroosmotic flow pump (LVSEP) with transient isotachophoresis (tITP) was applied to microchip electrophoresis (MCE) for improving both detection sensitivities and peak shapes. To realize LDIS in MCE, we investigated experimental procedures for injecting a short plug of a leading electrolyte (LE) solution into a straight microchannel without any sophisticated injector apparatus. We found that a short LE plug could be injected into a s...
Source: Anal Sci - May 16, 2024 Category: Chemistry Authors: Fumihiko Kitagawa Kazuki Takahashi Reina Osanai Ryota Sasaki Takayuki Kawai Source Type: research

Revolutionizing scandium detection in real samples: Unleashing the power of sol-gel-based optical sensor
Anal Sci. 2024 May 15. doi: 10.1007/s44211-024-00589-5. Online ahead of print.ABSTRACTThe development of a highly selective and ultra-sensitive optical sensor for detecting scandium (Sc3+) ions involves incorporating the reagent 2,3-dichloro-6-(3-carboxy-2-hydroxy-1-naphthylazo)quinoxaline (DCHNAQ) into a silica sol-gel thin film on a glass substrate. This innovative approach utilizes tetraethoxy-silane (TEOS) as the precursor, maintaining a sol-gel pH level of 4.5, a water-to-alkoxide ratio of 5:1, and a DCHNAQ concentration of 5.0 × 10-4 M. A detailed exploration of the impact of sol-gel parameters on the sensing capabi...
Source: Anal Sci - May 15, 2024 Category: Chemistry Authors: Salah M El-Bahy Abdullah A A Sari Alaa S Amin Mohamed A Ali Source Type: research

In vitro evaluation of novel SN-38 prodrug activated by α-rhamnosidase of exogenous enzyme
This study introduces the α-rhamnose (Rham)-conjugated prodrug of SN-38 (Rham-SN-38) as a promising alternative to irinotecan. α-rhamnosidase, responsible for SN-38 release from Rham-SN-38, does not express in human cells, minimizing individual variability and side effects. The injection of the α-rhamnosidase into the tumor tissues makes it possible, for the first time, to activate the Rham-SN-38. Furthermore, α-rhamnosidase demonstrates significantly higher activity than carboxylesterase, the specific enzyme activating irinotecan. SN-38 release mediated by α-rhamnosidase completes within 2 h, with a kcat/Km value app...
Source: Anal Sci - May 15, 2024 Category: Chemistry Authors: Teruki Nii Shoichi Hijii Ryosuke Kaneko Kenta Tanito Kota Yamanaka Akihiro Kishimura Takeshi Mori Yoshiki Katayama Source Type: research

Drug use and condomless sex among sexual minority men in relationships: Whether relationship quality is a risk or protective factor depends upon what they believe their partners do
This study tested the hypotheses that perceptions of partner (cannabis and illicit) drug use and sexual behavior would predict personal drug use and sexual behavior as well as moderate the association between relationship quality and these health outcomes. Recruitment via social networking applications yielded a sample of 5511 cisgender SMM aged 18-85 (M = 38.21, SD = 12.16) who were in a relationship with an adult cisgender male main partner. Respondents completed an online survey assessing their own cannabis use, illicit drug use, and condomless anal sex (CAS) with casual partners during the past 30 days as well as their...
Source: Anal Sci - May 15, 2024 Category: Chemistry Authors: Joseph R Hillesheim Tyrel J Starks Source Type: research

Revolutionizing scandium detection in real samples: Unleashing the power of sol-gel-based optical sensor
Anal Sci. 2024 May 15. doi: 10.1007/s44211-024-00589-5. Online ahead of print.ABSTRACTThe development of a highly selective and ultra-sensitive optical sensor for detecting scandium (Sc3+) ions involves incorporating the reagent 2,3-dichloro-6-(3-carboxy-2-hydroxy-1-naphthylazo)quinoxaline (DCHNAQ) into a silica sol-gel thin film on a glass substrate. This innovative approach utilizes tetraethoxy-silane (TEOS) as the precursor, maintaining a sol-gel pH level of 4.5, a water-to-alkoxide ratio of 5:1, and a DCHNAQ concentration of 5.0 × 10-4 M. A detailed exploration of the impact of sol-gel parameters on the sensing capabi...
Source: Anal Sci - May 15, 2024 Category: Chemistry Authors: Salah M El-Bahy Abdullah A A Sari Alaa S Amin Mohamed A Ali Source Type: research

In vitro evaluation of novel SN-38 prodrug activated by α-rhamnosidase of exogenous enzyme
This study introduces the α-rhamnose (Rham)-conjugated prodrug of SN-38 (Rham-SN-38) as a promising alternative to irinotecan. α-rhamnosidase, responsible for SN-38 release from Rham-SN-38, does not express in human cells, minimizing individual variability and side effects. The injection of the α-rhamnosidase into the tumor tissues makes it possible, for the first time, to activate the Rham-SN-38. Furthermore, α-rhamnosidase demonstrates significantly higher activity than carboxylesterase, the specific enzyme activating irinotecan. SN-38 release mediated by α-rhamnosidase completes within 2 h, with a kcat/Km value app...
Source: Anal Sci - May 15, 2024 Category: Chemistry Authors: Teruki Nii Shoichi Hijii Ryosuke Kaneko Kenta Tanito Kota Yamanaka Akihiro Kishimura Takeshi Mori Yoshiki Katayama Source Type: research