LC-MS/MS analysis of components in smoke from e-cigarettes that use guarana extract as the caffeine source
In this study, an LC-MS/MS analysis of components in the smoke from e-cigarettes with GE was performed. The concentration ranges of Caf and the minor components theophylline (TP), theobromine (TB), and paraxanthine (PX) in e-liquid and cigarette smoke extract (CSE) of five e-cigarette products were determined. The concentration ranges of e-liquid and CSE were 2.17-8.62 mg/mL and 0.17-1.17 µg/puff for Caf, 0.09-37.58 µg/mL and 0.03-11.88 ng/puff for TB, 50.28-185.26 ng/mL and 0.00-0.05 ng/puff for TP, and 0.44-4.09 µg/mL and 0.03-0.20 ng/puff for PX, respectively. By comparing the peak area ratios of e-liquid and CSE, we...
Source: Anal Sci - March 20, 2024 Category: Chemistry Authors: Suzuna Saruwatari Makoto Takada Junpei Mutoh Naoya Kishikawa Naotaka Kuroda Mitsuhiro Wada Source Type: research
Tag-free protein modification by lipoate ligase A: exploring substrate tolerance
This study delves into the functional intricacies of lipoate ligase A (LplA), an enzyme showing great promise in bioconjugation due to its unique capacity for introducing azido groups into proteins without requiring a genetic tag. We aimed to enhance the understanding of LplA's functionality, particularly its substrate tolerance and the reliability of various analytical techniques. A pivotal aspect of our approach was incorporating azido groups into a range of proteins, followed by the addition of the fluorescent molecule Cy3 via click chemistry. Analysis of fluorescent intensity in the altered proteins indicated varying d...
Source: Anal Sci - March 20, 2024 Category: Chemistry Authors: Shunsuke Yamazaki Kazutoshi Takahashi Yutaka Matsuda Source Type: research
LC-MS/MS analysis of components in smoke from e-cigarettes that use guarana extract as the caffeine source
In this study, an LC-MS/MS analysis of components in the smoke from e-cigarettes with GE was performed. The concentration ranges of Caf and the minor components theophylline (TP), theobromine (TB), and paraxanthine (PX) in e-liquid and cigarette smoke extract (CSE) of five e-cigarette products were determined. The concentration ranges of e-liquid and CSE were 2.17-8.62 mg/mL and 0.17-1.17 µg/puff for Caf, 0.09-37.58 µg/mL and 0.03-11.88 ng/puff for TB, 50.28-185.26 ng/mL and 0.00-0.05 ng/puff for TP, and 0.44-4.09 µg/mL and 0.03-0.20 ng/puff for PX, respectively. By comparing the peak area ratios of e-liquid and CSE, we...
Source: Anal Sci - March 20, 2024 Category: Chemistry Authors: Suzuna Saruwatari Makoto Takada Junpei Mutoh Naoya Kishikawa Naotaka Kuroda Mitsuhiro Wada Source Type: research
Competition, cooperation, and parental effects in larval aggregations formed on carrion by communally breeding beetles Necrodes littoralis (Staphylinidae: Silphinae)
Insect Sci. 2024 Mar 13. doi: 10.1111/1744-7917.13353. Online ahead of print.ABSTRACTAggregations of juveniles are dominant forms of social life in some insect groups. Larval societies are shaped by competitive and cooperative interactions of the larvae, in parallel with parental effects. Colonies of necrophagous larvae are excellent systems to study these relationships. Necrodes littoralis (Staphylinidae: Silphinae), a carrion beetle that colonizes cadavers of large vertebrates, forms massive juvenile aggregations. By spreading over carrion anal and oral exudates, the beetles form the feeding matrix, in which the heat is ...
Source: Anal Sci - March 14, 2024 Category: Chemistry Authors: Natalia Lis Anna M ądra-Bielewicz J ędrzej Wydra Szymon Matuszewski Source Type: research
Aspirin-Mediated Acetylation of SIRT1 Maintains Intestinal Immune Homeostasis
Adv Sci (Weinh). 2024 Mar 14:e2306378. doi: 10.1002/advs.202306378. Online ahead of print.ABSTRACTAspirin, also named acetylsalicylate, can directly acetylate the side-chain of lysine in protein, which leads to the possibility of unexplained drug effects. Here, the study used isotopic-labeling aspirin-d3 with mass spectrometry analysis to discover that aspirin directly acetylates 10 HDACs proteins, including SIRT1, the most studied NAD+ -dependent deacetylase. SIRT1 is also acetylated by aspirin in vitro. It is also identified that aspirin directly acetylates lysine 408 of SIRT1, which abolishes SIRT1 deacetylation activit...
Source: Anal Sci - March 14, 2024 Category: Chemistry Authors: Liangguo Xie Chaoqun Li Chao Wang Zhen Wu Changchun Wang Chunyu Chen Xiaojian Chen Dejian Zhou Qiang Zhou Ping Lu Chen Ding Chen-Ying Liu Jinzhong Lin Xumin Zhang Xiaofei Yu Wei Yu Source Type: research
Itemized determination of As, Sb, Bi, Se, and Ge in graphite sample by HG-AFS with high-temperature microwave digestion
Anal Sci. 2024 Mar 14. doi: 10.1007/s44211-024-00526-6. Online ahead of print.ABSTRACTIn this paper, we optimized a method for fast and accurate determination of five impurity elements (As, Sb, Bi, Se, and Ge) in graphite samples to overcome the shortcomings of existing methods, such as complicated equipment, cumbersome process, multiple-time preparation, separate determination, and large error in results. Graphite samples were digested with HNO3-H2SO4-HClO4-HF in a high-temperature and high-pressure microwave digestion apparatus, and the elements were extracted and determined separately by AFS (atomic fluorescence spectro...
Source: Anal Sci - March 14, 2024 Category: Chemistry Authors: Jian-Wei Meng Li-Ping Wang Ai-Qin Liu Yan-Qiao Hu Li Wei Chao Chen Xue-Mei Zhang Liang-Cheng Zhao Hua-Jie Liu Meng-Hua Sun Source Type: research
Competition, cooperation, and parental effects in larval aggregations formed on carrion by communally breeding beetles Necrodes littoralis (Staphylinidae: Silphinae)
Insect Sci. 2024 Mar 13. doi: 10.1111/1744-7917.13353. Online ahead of print.ABSTRACTAggregations of juveniles are dominant forms of social life in some insect groups. Larval societies are shaped by competitive and cooperative interactions of the larvae, in parallel with parental effects. Colonies of necrophagous larvae are excellent systems to study these relationships. Necrodes littoralis (Staphylinidae: Silphinae), a carrion beetle that colonizes cadavers of large vertebrates, forms massive juvenile aggregations. By spreading over carrion anal and oral exudates, the beetles form the feeding matrix, in which the heat is ...
Source: Anal Sci - March 14, 2024 Category: Chemistry Authors: Natalia Lis Anna M ądra-Bielewicz J ędrzej Wydra Szymon Matuszewski Source Type: research
Aspirin-Mediated Acetylation of SIRT1 Maintains Intestinal Immune Homeostasis
Adv Sci (Weinh). 2024 Mar 14:e2306378. doi: 10.1002/advs.202306378. Online ahead of print.ABSTRACTAspirin, also named acetylsalicylate, can directly acetylate the side-chain of lysine in protein, which leads to the possibility of unexplained drug effects. Here, the study used isotopic-labeling aspirin-d3 with mass spectrometry analysis to discover that aspirin directly acetylates 10 HDACs proteins, including SIRT1, the most studied NAD+ -dependent deacetylase. SIRT1 is also acetylated by aspirin in vitro. It is also identified that aspirin directly acetylates lysine 408 of SIRT1, which abolishes SIRT1 deacetylation activit...
Source: Anal Sci - March 14, 2024 Category: Chemistry Authors: Liangguo Xie Chaoqun Li Chao Wang Zhen Wu Changchun Wang Chunyu Chen Xiaojian Chen Dejian Zhou Qiang Zhou Ping Lu Chen Ding Chen-Ying Liu Jinzhong Lin Xumin Zhang Xiaofei Yu Wei Yu Source Type: research
Itemized determination of As, Sb, Bi, Se, and Ge in graphite sample by HG-AFS with high-temperature microwave digestion
Anal Sci. 2024 Mar 14. doi: 10.1007/s44211-024-00526-6. Online ahead of print.ABSTRACTIn this paper, we optimized a method for fast and accurate determination of five impurity elements (As, Sb, Bi, Se, and Ge) in graphite samples to overcome the shortcomings of existing methods, such as complicated equipment, cumbersome process, multiple-time preparation, separate determination, and large error in results. Graphite samples were digested with HNO3-H2SO4-HClO4-HF in a high-temperature and high-pressure microwave digestion apparatus, and the elements were extracted and determined separately by AFS (atomic fluorescence spectro...
Source: Anal Sci - March 14, 2024 Category: Chemistry Authors: Jian-Wei Meng Li-Ping Wang Ai-Qin Liu Yan-Qiao Hu Li Wei Chao Chen Xue-Mei Zhang Liang-Cheng Zhao Hua-Jie Liu Meng-Hua Sun Source Type: research
Competition, cooperation, and parental effects in larval aggregations formed on carrion by communally breeding beetles Necrodes littoralis (Staphylinidae: Silphinae)
Insect Sci. 2024 Mar 13. doi: 10.1111/1744-7917.13353. Online ahead of print.ABSTRACTAggregations of juveniles are dominant forms of social life in some insect groups. Larval societies are shaped by competitive and cooperative interactions of the larvae, in parallel with parental effects. Colonies of necrophagous larvae are excellent systems to study these relationships. Necrodes littoralis (Staphylinidae: Silphinae), a carrion beetle that colonizes cadavers of large vertebrates, forms massive juvenile aggregations. By spreading over carrion anal and oral exudates, the beetles form the feeding matrix, in which the heat is ...
Source: Anal Sci - March 14, 2024 Category: Chemistry Authors: Natalia Lis Anna M ądra-Bielewicz J ędrzej Wydra Szymon Matuszewski Source Type: research
Aspirin-Mediated Acetylation of SIRT1 Maintains Intestinal Immune Homeostasis
Adv Sci (Weinh). 2024 Mar 14:e2306378. doi: 10.1002/advs.202306378. Online ahead of print.ABSTRACTAspirin, also named acetylsalicylate, can directly acetylate the side-chain of lysine in protein, which leads to the possibility of unexplained drug effects. Here, the study used isotopic-labeling aspirin-d3 with mass spectrometry analysis to discover that aspirin directly acetylates 10 HDACs proteins, including SIRT1, the most studied NAD+ -dependent deacetylase. SIRT1 is also acetylated by aspirin in vitro. It is also identified that aspirin directly acetylates lysine 408 of SIRT1, which abolishes SIRT1 deacetylation activit...
Source: Anal Sci - March 14, 2024 Category: Chemistry Authors: Liangguo Xie Chaoqun Li Chao Wang Zhen Wu Changchun Wang Chunyu Chen Xiaojian Chen Dejian Zhou Qiang Zhou Ping Lu Chen Ding Chen-Ying Liu Jinzhong Lin Xumin Zhang Xiaofei Yu Wei Yu Source Type: research
Itemized determination of As, Sb, Bi, Se, and Ge in graphite sample by HG-AFS with high-temperature microwave digestion
Anal Sci. 2024 Mar 14. doi: 10.1007/s44211-024-00526-6. Online ahead of print.ABSTRACTIn this paper, we optimized a method for fast and accurate determination of five impurity elements (As, Sb, Bi, Se, and Ge) in graphite samples to overcome the shortcomings of existing methods, such as complicated equipment, cumbersome process, multiple-time preparation, separate determination, and large error in results. Graphite samples were digested with HNO3-H2SO4-HClO4-HF in a high-temperature and high-pressure microwave digestion apparatus, and the elements were extracted and determined separately by AFS (atomic fluorescence spectro...
Source: Anal Sci - March 14, 2024 Category: Chemistry Authors: Jian-Wei Meng Li-Ping Wang Ai-Qin Liu Yan-Qiao Hu Li Wei Chao Chen Xue-Mei Zhang Liang-Cheng Zhao Hua-Jie Liu Meng-Hua Sun Source Type: research
Competition, cooperation, and parental effects in larval aggregations formed on carrion by communally breeding beetles Necrodes littoralis (Staphylinidae: Silphinae)
Insect Sci. 2024 Mar 13. doi: 10.1111/1744-7917.13353. Online ahead of print.ABSTRACTAggregations of juveniles are dominant forms of social life in some insect groups. Larval societies are shaped by competitive and cooperative interactions of the larvae, in parallel with parental effects. Colonies of necrophagous larvae are excellent systems to study these relationships. Necrodes littoralis (Staphylinidae: Silphinae), a carrion beetle that colonizes cadavers of large vertebrates, forms massive juvenile aggregations. By spreading over carrion anal and oral exudates, the beetles form the feeding matrix, in which the heat is ...
Source: Anal Sci - March 14, 2024 Category: Chemistry Authors: Natalia Lis Anna M ądra-Bielewicz J ędrzej Wydra Szymon Matuszewski Source Type: research
Aspirin-Mediated Acetylation of SIRT1 Maintains Intestinal Immune Homeostasis
Adv Sci (Weinh). 2024 Mar 14:e2306378. doi: 10.1002/advs.202306378. Online ahead of print.ABSTRACTAspirin, also named acetylsalicylate, can directly acetylate the side-chain of lysine in protein, which leads to the possibility of unexplained drug effects. Here, the study used isotopic-labeling aspirin-d3 with mass spectrometry analysis to discover that aspirin directly acetylates 10 HDACs proteins, including SIRT1, the most studied NAD+ -dependent deacetylase. SIRT1 is also acetylated by aspirin in vitro. It is also identified that aspirin directly acetylates lysine 408 of SIRT1, which abolishes SIRT1 deacetylation activit...
Source: Anal Sci - March 14, 2024 Category: Chemistry Authors: Liangguo Xie Chaoqun Li Chao Wang Zhen Wu Changchun Wang Chunyu Chen Xiaojian Chen Dejian Zhou Qiang Zhou Ping Lu Chen Ding Chen-Ying Liu Jinzhong Lin Xumin Zhang Xiaofei Yu Wei Yu Source Type: research
Itemized determination of As, Sb, Bi, Se, and Ge in graphite sample by HG-AFS with high-temperature microwave digestion
Anal Sci. 2024 Mar 14. doi: 10.1007/s44211-024-00526-6. Online ahead of print.ABSTRACTIn this paper, we optimized a method for fast and accurate determination of five impurity elements (As, Sb, Bi, Se, and Ge) in graphite samples to overcome the shortcomings of existing methods, such as complicated equipment, cumbersome process, multiple-time preparation, separate determination, and large error in results. Graphite samples were digested with HNO3-H2SO4-HClO4-HF in a high-temperature and high-pressure microwave digestion apparatus, and the elements were extracted and determined separately by AFS (atomic fluorescence spectro...
Source: Anal Sci - March 14, 2024 Category: Chemistry Authors: Jian-Wei Meng Li-Ping Wang Ai-Qin Liu Yan-Qiao Hu Li Wei Chao Chen Xue-Mei Zhang Liang-Cheng Zhao Hua-Jie Liu Meng-Hua Sun Source Type: research
