Impact of activity-based training on bowel function in a rat model of spinal cord injury
J Neurotrauma. 2023 Dec 20. doi: 10.1089/neu.2023.0486. Online ahead of print.ABSTRACTSignificant bowel-related issues after spinal cord injury (SCI) that affect morbidity and quality of life include diminished bowel motility, loss of sphincter control, gastric ulcers, autonomic dysreflexia, pain, diarrhea, constipation, and fecal incontinence. Clinical diagnoses and research in humans have largely relied on anorectal manometry (ARM) procedures to increase understanding of the functional effects of SCI on colorectal motility and defecation physiology. Recent pre-clinical rodent studies have also used ARM to further our und...
Source: Anal Sci - December 20, 2023 Category: Chemistry Authors: Jason Fell Daniel Medina Aguinaga Darlene A Burke Charles H Hubscher Source Type: research
Comparison of 3-nitrophenylhydrazine, O-benzyl hydroxylamine, and 2-picolylamine derivatizations for analysis of short-chain fatty acids through liquid chromatography coupled with tandem mass spectrometry
Anal Sci. 2023 Dec 19. doi: 10.1007/s44211-023-00474-7. Online ahead of print.ABSTRACTShort-chain fatty acids (SCFAs) are metabolites derived from gut microbiota and implicated in host homeostasis. Hence, the profiling SCFAs from biological samples plays an important role in revealing the interaction between gut microbiota and pathogens. Previous studies, liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with various derivatization strategies have been performed to obtain the SCFA profiles from biological samples. However, it is poor evidence to compare these derivatization regents and conditions. Thus, we...
Source: Anal Sci - December 19, 2023 Category: Chemistry Authors: Ryosuke Nagatomo Aoi Ichikawa Haruki Kaneko Koichi Inoue Source Type: research
Targeted-activation superparamagnetic spherical nucleic acid nanomachine for ultrasensitive SERS detection of lysozyme based on a bienzymatic-mediated in situ amplification strategy
Anal Sci. 2023 Dec 19. doi: 10.1007/s44211-023-00471-w. Online ahead of print.ABSTRACTLysozyme (LYS) is a widely used bacteriostatic enzyme. In this paper, we built a sensitive and accurate Raman biosensing platform to detect trace amounts of LYS. The method is based on magnetic spherical nucleic acid formed by a combination of LYS aptamer (Apt) and magnetic beads (MBs). Meanwhile, this method utilizes a dual enzyme-assisted nucleic acid amplification circuit and surface-enhanced Raman scattering (SERS). In this sensing strategy, which is based on the specific recognition of Apt, magnetic spherical nucleic acids were assoc...
Source: Anal Sci - December 19, 2023 Category: Chemistry Authors: Yang Xu Yue Liu Yu Luo Xinlin Xu Yingying Li Lin Zhao Tiantian Li Yan Zhang Peng He Xiaoming Mou Source Type: research
Validation of spectrophotometric and colorimetric methods to quantify clindamycin in skin tissue: application to in vitro release and ex vivo dermatokinetic studies from separable effervescent microarray patch loaded bacterially sensitive microparticle
Anal Sci. 2023 Dec 19. doi: 10.1007/s44211-023-00478-3. Online ahead of print.ABSTRACTDiabetes mellitus can cause diabetic foot infection (DFI) complications. DFI is generally caused by infection from bacteria and Methicillin-Resistant Staphylococcus aureus (MRSA) which is resistant to several antibiotics. Application therapy of clindamycin (CLY) administration with the oral route has low bioavailability and non-selective distribution of antibiotics towards bacteria intravenously. In this research, CLY was developed into bacterially sensitive microparticles (MPs) which were further incorporated into a separable effervescen...
Source: Anal Sci - December 19, 2023 Category: Chemistry Authors: Iis Nurul Rahmadani Nurul Fauziah Muhammad Nur Hidayat Nur Annisa Safirah Nur Azizah Fadhilah Andi Dian Permana Source Type: research
Evolution and chemical diversity of the volatile compounds in Salvia species
CONCLUSION: This study offered new insights into the phylogenetic relationships besides chemistry diversity and distribution pattern of volatile compounds of genus Salvia, providing theoretical guidance for the investigations and development of secondary metabolites.PMID:38114450 | DOI:10.1002/pca.3306 (Source: Anal Sci)
Source: Anal Sci - December 19, 2023 Category: Chemistry Authors: Feiyan Wang Yanbo Huang Zuoni Hou Yue Chen Ganggui Lou Zhechen Qi Xiaodan Zhang Mans Dennis Lei Zhang Yukun Wei Dongfeng Yang Source Type: research
Effect of Perineal Massage and Warm Compresses Technique in Postpartum Pelvic Floor Dysfunction. A Secondary Analysis from a Randomised Controlled Trial
Reprod Sci. 2023 Dec 14. doi: 10.1007/s43032-023-01424-4. Online ahead of print.ABSTRACTPelvic floor dysfunction (PFD) is common to be associated with pregnancy and birth. To date, no research has been done to understand whether the perineal massage and warm compresses technique has an impact on pelvic floor dysfunction. To assess the impact of perineal massage and warm compresses technique during the second stage of labor in pelvic floor dysfunction at 3 and 6 months postpartum. Of the 800 women recruited to randomised controlled trial to prevent perineal trauma, 496 were included in the study, with 242 (48.8%) assigned t...
Source: Anal Sci - December 15, 2023 Category: Chemistry Authors: Silvia Rodrigues Paulo Silva Ana Catarina Borges Natacha Quintal de Sousa Jo ão Neves Silva Ramon Escuriet Source Type: research
Effect of Perineal Massage and Warm Compresses Technique in Postpartum Pelvic Floor Dysfunction. A Secondary Analysis from a Randomised Controlled Trial
Reprod Sci. 2023 Dec 14. doi: 10.1007/s43032-023-01424-4. Online ahead of print.ABSTRACTPelvic floor dysfunction (PFD) is common to be associated with pregnancy and birth. To date, no research has been done to understand whether the perineal massage and warm compresses technique has an impact on pelvic floor dysfunction. To assess the impact of perineal massage and warm compresses technique during the second stage of labor in pelvic floor dysfunction at 3 and 6 months postpartum. Of the 800 women recruited to randomised controlled trial to prevent perineal trauma, 496 were included in the study, with 242 (48.8%) assigned t...
Source: Anal Sci - December 15, 2023 Category: Chemistry Authors: Silvia Rodrigues Paulo Silva Ana Catarina Borges Natacha Quintal de Sousa Jo ão Neves Silva Ramon Escuriet Source Type: research
Effect of Perineal Massage and Warm Compresses Technique in Postpartum Pelvic Floor Dysfunction. A Secondary Analysis from a Randomised Controlled Trial
Reprod Sci. 2023 Dec 14. doi: 10.1007/s43032-023-01424-4. Online ahead of print.ABSTRACTPelvic floor dysfunction (PFD) is common to be associated with pregnancy and birth. To date, no research has been done to understand whether the perineal massage and warm compresses technique has an impact on pelvic floor dysfunction. To assess the impact of perineal massage and warm compresses technique during the second stage of labor in pelvic floor dysfunction at 3 and 6 months postpartum. Of the 800 women recruited to randomised controlled trial to prevent perineal trauma, 496 were included in the study, with 242 (48.8%) assigned t...
Source: Anal Sci - December 15, 2023 Category: Chemistry Authors: Silvia Rodrigues Paulo Silva Ana Catarina Borges Natacha Quintal de Sousa Jo ão Neves Silva Ramon Escuriet Source Type: research
Effect of Perineal Massage and Warm Compresses Technique in Postpartum Pelvic Floor Dysfunction. A Secondary Analysis from a Randomised Controlled Trial
Reprod Sci. 2023 Dec 14. doi: 10.1007/s43032-023-01424-4. Online ahead of print.ABSTRACTPelvic floor dysfunction (PFD) is common to be associated with pregnancy and birth. To date, no research has been done to understand whether the perineal massage and warm compresses technique has an impact on pelvic floor dysfunction. To assess the impact of perineal massage and warm compresses technique during the second stage of labor in pelvic floor dysfunction at 3 and 6 months postpartum. Of the 800 women recruited to randomised controlled trial to prevent perineal trauma, 496 were included in the study, with 242 (48.8%) assigned t...
Source: Anal Sci - December 15, 2023 Category: Chemistry Authors: Silvia Rodrigues Paulo Silva Ana Catarina Borges Natacha Quintal de Sousa Jo ão Neves Silva Ramon Escuriet Source Type: research
Effect of Perineal Massage and Warm Compresses Technique in Postpartum Pelvic Floor Dysfunction. A Secondary Analysis from a Randomised Controlled Trial
Reprod Sci. 2023 Dec 14. doi: 10.1007/s43032-023-01424-4. Online ahead of print.ABSTRACTPelvic floor dysfunction (PFD) is common to be associated with pregnancy and birth. To date, no research has been done to understand whether the perineal massage and warm compresses technique has an impact on pelvic floor dysfunction. To assess the impact of perineal massage and warm compresses technique during the second stage of labor in pelvic floor dysfunction at 3 and 6 months postpartum. Of the 800 women recruited to randomised controlled trial to prevent perineal trauma, 496 were included in the study, with 242 (48.8%) assigned t...
Source: Anal Sci - December 15, 2023 Category: Chemistry Authors: Silvia Rodrigues Paulo Silva Ana Catarina Borges Natacha Quintal de Sousa Jo ão Neves Silva Ramon Escuriet Source Type: research
Analysis of dog meat adulteration in beef meatballs using non-targeted UHPLC-Orbitrap HRMS metabolomics and chemometrics for halal authentication study
The objective of this research was to develop an analytical method for halal authentication testing of beef meatballs (BM) from dog meat (DM) using a non-targeted metabolomics approach employing liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and chemometrics. The differentiation of authentic BM from that adulterated with DM was successfully performed using partial least square-discriminant analysis (PLS-DA) with high accuracy (R2X = 0.980, and R2Y = 0.980) and good predictivity (Q2 = 0.517). In addition, partial least square (PLS) and orthogonal PLS (OPLS) were successfully used to predict the DM added (...
Source: Anal Sci - December 14, 2023 Category: Chemistry Authors: Anjar Windarsih Nor Kartini Abu Bakar Abdul Rohman Yuny Erwanto Source Type: research
Deployment and assessment of a deep learning model for real-time detection of anal precancer with high frame rate high-resolution microendoscopy
Sci Rep. 2023 Dec 14;13(1):22267. doi: 10.1038/s41598-023-49197-9.ABSTRACTAnal cancer incidence is significantly higher in people living with HIV as HIV increases the oncogenic potential of human papillomavirus. The incidence of anal cancer in the United States has recently increased, with diagnosis and treatment hampered by high loss-to-follow-up rates. Novel methods for the automated, real-time diagnosis of AIN 2+ could enable "see and treat" strategies, reducing loss-to-follow-up rates. A previous retrospective study demonstrated that the accuracy of a high-resolution microendoscope (HRME) coupled with a deep learning m...
Source: Anal Sci - December 14, 2023 Category: Chemistry Authors: David Brenes Alex Kortum Jackson Coole Jennifer Carns Richard Schwarz Imran Vohra Rebecca Richards-Kortum Yuxin Liu Zhenjian Cai Keith Sigel Sharmila Anandasabapathy Michael Gaisa Elizabeth Chiao Source Type: research
Simultaneous speciation analysis of arsenic and iodine in human urine by high performance liquid chromatography-inductively coupled plasma mass spectrometry
Anal Sci. 2023 Dec 13. doi: 10.1007/s44211-023-00472-9. Online ahead of print.ABSTRACTA high-performance liquid chromatography-inductively coupled plasma mass spectrometry-based method was developed for the simultaneous determination of four iodine species (i.e. iodate, 3-iodo-tyrosine, 3,5-diiodo-tyrosine, and iodide) and six arsenic species (i.e. arsenobetaine, arsenite, dimethylarsinic acid, arsenocholine, methylarsonic acid, and arsenate) in human urine. The chromatographic separation was performed on a Dionex IonPac As7 anion exchange column. The mobile phase was initiated with 0.5 mmol/L ammonium carbonate solution, ...
Source: Anal Sci - December 13, 2023 Category: Chemistry Authors: Shaomin Zhou Heng Wang Jun Tang Heng Wang Jianbo Yan Source Type: research
A fluorescence-based binding assay for proteins using the cell surface as a sensing platform
Anal Sci. 2023 Dec 13. doi: 10.1007/s44211-023-00476-5. Online ahead of print.ABSTRACTProtein-protein interaction (PPI) analysis is very important for elucidating the functions of proteins because many proteins execute their functions in living cells by interacting with one another. In PPI analysis, methods using the sensor chips are widely employed to obtain quantitative data. However, these methods require that the target proteins be immobilized on the sensor chips, and the immobilization processes can affect the binding of the target proteins to their binding partners. In the present work, we propose a PPI analysis syst...
Source: Anal Sci - December 13, 2023 Category: Chemistry Authors: Kazuki Hirano Shinji Sueda Source Type: research
Solidification characteristics of solid biofuel densified by two-step torrefaction process
In this study, an innovative densification molding method is proposed; it uses a two-step torrefaction process with a high CO2 reduction effect. The molding method consists of the following two-step torrefaction process at torrefaction temperatures of 463-773 K: In the first step, raw biomass is torrefied to remove some of the volatile matter that inhibits densified molding. In the second step, the torrefied biomass is densified at the above temperature. The purpose of the second torrefaction step is to further enhance the fuel ratio due to the conversion of volatile matter to fixed carbon and to develop the thermal soften...
Source: Anal Sci - December 13, 2023 Category: Chemistry Authors: Takahiro Murakami Satoru Mizuno Toru Sawai Source Type: research
