A fluorescence-based binding assay for proteins using the cell surface as a sensing platform
Anal Sci. 2023 Dec 13. doi: 10.1007/s44211-023-00476-5. Online ahead of print.ABSTRACTProtein-protein interaction (PPI) analysis is very important for elucidating the functions of proteins because many proteins execute their functions in living cells by interacting with one another. In PPI analysis, methods using the sensor chips are widely employed to obtain quantitative data. However, these methods require that the target proteins be immobilized on the sensor chips, and the immobilization processes can affect the binding of the target proteins to their binding partners. In the present work, we propose a PPI analysis syst...
Source: Anal Sci - December 13, 2023 Category: Chemistry Authors: Kazuki Hirano Shinji Sueda Source Type: research
Solidification characteristics of solid biofuel densified by two-step torrefaction process
In this study, an innovative densification molding method is proposed; it uses a two-step torrefaction process with a high CO2 reduction effect. The molding method consists of the following two-step torrefaction process at torrefaction temperatures of 463-773 K: In the first step, raw biomass is torrefied to remove some of the volatile matter that inhibits densified molding. In the second step, the torrefied biomass is densified at the above temperature. The purpose of the second torrefaction step is to further enhance the fuel ratio due to the conversion of volatile matter to fixed carbon and to develop the thermal soften...
Source: Anal Sci - December 13, 2023 Category: Chemistry Authors: Takahiro Murakami Satoru Mizuno Toru Sawai Source Type: research
Simultaneous speciation analysis of arsenic and iodine in human urine by high performance liquid chromatography-inductively coupled plasma mass spectrometry
Anal Sci. 2023 Dec 13. doi: 10.1007/s44211-023-00472-9. Online ahead of print.ABSTRACTA high-performance liquid chromatography-inductively coupled plasma mass spectrometry-based method was developed for the simultaneous determination of four iodine species (i.e. iodate, 3-iodo-tyrosine, 3,5-diiodo-tyrosine, and iodide) and six arsenic species (i.e. arsenobetaine, arsenite, dimethylarsinic acid, arsenocholine, methylarsonic acid, and arsenate) in human urine. The chromatographic separation was performed on a Dionex IonPac As7 anion exchange column. The mobile phase was initiated with 0.5 mmol/L ammonium carbonate solution, ...
Source: Anal Sci - December 13, 2023 Category: Chemistry Authors: Shaomin Zhou Heng Wang Jun Tang Heng Wang Jianbo Yan Source Type: research
A fluorescence-based binding assay for proteins using the cell surface as a sensing platform
Anal Sci. 2023 Dec 13. doi: 10.1007/s44211-023-00476-5. Online ahead of print.ABSTRACTProtein-protein interaction (PPI) analysis is very important for elucidating the functions of proteins because many proteins execute their functions in living cells by interacting with one another. In PPI analysis, methods using the sensor chips are widely employed to obtain quantitative data. However, these methods require that the target proteins be immobilized on the sensor chips, and the immobilization processes can affect the binding of the target proteins to their binding partners. In the present work, we propose a PPI analysis syst...
Source: Anal Sci - December 13, 2023 Category: Chemistry Authors: Kazuki Hirano Shinji Sueda Source Type: research
Solidification characteristics of solid biofuel densified by two-step torrefaction process
In this study, an innovative densification molding method is proposed; it uses a two-step torrefaction process with a high CO2 reduction effect. The molding method consists of the following two-step torrefaction process at torrefaction temperatures of 463-773 K: In the first step, raw biomass is torrefied to remove some of the volatile matter that inhibits densified molding. In the second step, the torrefied biomass is densified at the above temperature. The purpose of the second torrefaction step is to further enhance the fuel ratio due to the conversion of volatile matter to fixed carbon and to develop the thermal soften...
Source: Anal Sci - December 13, 2023 Category: Chemistry Authors: Takahiro Murakami Satoru Mizuno Toru Sawai Source Type: research
