Functional expression of the free fatty acids receptor-1 and -4 (FFA1/GPR40 and FFA4/GPR120) in bovine endometrial cells
In conclusion, endometrial cells express FFA4 and FFA1 receptors, and DHA induces intracellular calcium release via FFA4 and FFA1 receptors. DHA reduces PGE2, but this response was not mediated by FFA4 or FFA1 receptors. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - June 11, 2019 Category: Veterinary Research Source Type: research

Kinetics of the expression of CD163 and CD107a in the lung and tonsil of pigs after infection with PRRSV-1 strains of different virulence
This study assesses the expression, distribution and kinetics of PRRSV N-protein, CD163 and CD107a in the lung and tonsil from experimentally-infected piglets with three different PRRSV-1 strains: a virulent PRRSV-1 subtype 3 strain (SU1-bel) and two low-virulent subtype 1 strains, Lelystad virus (LV) and 215 –06. SU1-bel replicated more efficiently in the lungs and tonsils. The number of CD163+ cells decreased in both tissues from all infected groups at 7 dpi, followed by an increase at the end of the study, highlighting a negative correlation with the number of N-protein+-infected cells. A significant increase in C...
Source: Veterinary Research Communications - May 18, 2019 Category: Veterinary Research Source Type: research

RNA-Seq-based transcriptomic profiling of primary interstitial cells of Cajal in response to bovine viral diarrhea virus infection
This study is the first to establish a new infection model for investigating GI inflammatory lesions induced by BVDV infection. RNA-Seq-based transcriptomic profiling can provide a basis for study on BVDV-associated inflammatory lesions. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - May 18, 2019 Category: Veterinary Research Source Type: research

Intestinal gene expressions in broiler chickens infected with Escherichia coli and dietary supplemented with probiotic, acidifier and synbiotic
In this study, we investigated the effects of probiotic, acidifier and synbiotic supplementation on growth performance, mortality rate, intestinal gene expressions, fecal shedding, and organs colonization induced byEscherichia coli in broiler chickens. Six experimental groups were included; negative control group (NC), positive control group (PC), probiotic group (PR), acidifier group (AC), synbiotic group (SY) and colistin sulfate group (CS). Chickens in groups NC and PC were fed a basal diet, while chickens in groups PR, AC, SY, and CS were fed a basal diet containing probiotic, acidifier, synbiotic and colistin sulfate,...
Source: Veterinary Research Communications - May 4, 2019 Category: Veterinary Research Source Type: research

Quantification of EGFR family in canine mammary ductal carcinomas in situ: implications on the histological graduation
AbstractThe epithelial growth factor receptors are transmembrane proteins with an important role in the neoplastic progression of tumors, and in this context, DCIS is an important phase in the progression of canine mammary tumors. Studies on the molecular profile and its relationship to a progression of canine mammary tumors are important to improve the treatment of patients and for a better understanding of canine mammary carcinogenesis. The aim of this study was to determine, by immunohistochemistry, the relation between the expression of EGFR, ErbB-2, ErbB-3, and ErbB-4 in 52 canine mammary gland DCIS with high and low ...
Source: Veterinary Research Communications - April 24, 2019 Category: Veterinary Research Source Type: research

CD205-positive, Sepharose-induced peritoneal exudate cells: a new resource for DC research in the chicken
This study reports the development of new anti-CD205 monoclonal antibodies as a reagent for chicken DC research, as well as PEC as a potential source of CD205+ DC for ex vivo studies in the chicken. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - April 16, 2019 Category: Veterinary Research Source Type: research

Genetic association of polymorphisms in bovine TLR2 and TLR4 genes with Mycobacterium avium subspecies paratuberculosis infection in Indian cattle population
AbstractToll like receptors (TLRs) are pattern recognition molecules involved in cellular recognition ofMycobacterium avium subspeciesparatuberculosis (MAP), the infectious agent causing Paratuberculosis (PTB), a notified disease of domestic and wild ruminants. The present study was undertaken to investigate the presence of single nucleotide polymorphisms (SNPs) inTLR2 andTLR4 gene and to evaluate association of these SNPs with occurrence of PTB in Indian cattle. A total of 213 cattle, were subjected to multiple diagnostic tests viz. Johnin PPD, ELISA test (Indigenous and Parachek kit method), fecal microscopy and fecal cu...
Source: Veterinary Research Communications - March 28, 2019 Category: Veterinary Research Source Type: research

Ov2 is a modulator of OvHV-2 RTA mediated gene expression
AbstractOvine herpesvirus-2 (OvHV-2) is the causative agent of the sheep-associated form of malignant catarrhal fever, a usually fatal lymphoproliferative disease of bison, deer and cattle. Malignant catarrhal fever is a major cause of cattle loss in Africa with approximately 7% affected annually; and in North America has significant impact on bison farming. Research into the mechanisms by which OvHV-2 induces disease in susceptible species has been hampered by a lack of a cell culture system for the virus. Ov2 is a bZIP protein encoded by OvHV-2. Proteins with bZIP domains in other herpesviruses, such as the Kaposi &rsquo...
Source: Veterinary Research Communications - March 21, 2019 Category: Veterinary Research Source Type: research

Regulation of Ov2 by virus encoded microRNAs
AbstractHerpesviruses encode miRNAs that target both virus and host genes; however their role in herpesvirus biology is still poorly understood. We previously identified thirty five miRNAs encoded by OvHV-2; the causative agent of malignant catarrhal fever (MCF) and are investigating the role of these miRNAs in regulating expression of OvHV-2 genes that play important roles in virus biology. Analysis, using RNAHybrid predicted that two OvHV-2 encoded miRNAs, ovhv2-miR-17-10 and ovhv2-miR-61-1, target transcripts coding for the OvHV-2 bZIP protein Ov2. In other herpesvirus bZIP proteins are known to play important roles in ...
Source: Veterinary Research Communications - March 19, 2019 Category: Veterinary Research Source Type: research

Saccharomyces cerevisiae β-glucan-induced SBD-1 expression in ovine ruminal epithelial cells is mediated through the TLR-2-MyD88-NF-κB/MAPK pathway
AbstractOvine ruminal epithelial cells (ORECs) not only have a physical barrier function but also can secrete host defence peptides (HDPs), such as sheep β-defensin-1 (SBD-1). As a feed additive,Saccharomyces cerevisiae can enhance the host ’s innate immunity. β-glucan, a cell wall component ofSaccharomyces cerevisiae, can stimulate innate immune responses and trigger the up-regulation of SBD-1 in ORECs. The signaling mechanisms involved in β-glucan-induced SBD-1 expression are not completely understood. The aim of this study was to identify the receptors and intracellular pathways involved in the up-r...
Source: Veterinary Research Communications - March 12, 2019 Category: Veterinary Research Source Type: research

Unique genetic features of canine adenovirus type 1 (CAdV-1) infecting red foxes ( Vulpes vulpes ) in northern Norway and arctic foxes ( Vulpes lagopus ) in Svalbard
AbstractCanine adenovirus type 1 (CAdV-1) is the aetiological agent of infectious canine hepatitis (ICH) in domestic dogs (Canis familiaris). In spite of the widespread use of vaccination, CAdV-1 continues to circulate in the dog population. Although a high number of serological screenings have indicated that CAdV-1 is widespread in fox species, little is known about the potential role of foxes as reservoirs of CAdV-1. Furthermore, very little data exist on the molecular features of this virus in foxes. To add to existing knowledge on CAdV-1 circulating in wild carnivores, tissue samples from CAdV-seropositive red foxes (V...
Source: Veterinary Research Communications - March 2, 2019 Category: Veterinary Research Source Type: research

Localization profiles of natriuretic peptides in hearts of pre-hibernating and hibernating Anatolian ground squirrels ( Spermophilus xanthoprymnus )
AbstractThe Anatolian ground squirrel (Spermophilus xanthoprymnus) is a typical example of true mammalian hibernators. In order to adapt to extreme external and internal environments during hibernation, they lower their body temperatures, heart rates and oxygen consumption; however, pathological events such as ischemia and ventricular fibrillation do not occur in their cardiovascular systems. During the hibernation, maintenance of cardiac function is very important for survival of ground squirrels. Natriuretic peptides (NPs) are key factors in the regulation of cardiovascular hemostasis. Since NPs ’ role on the prote...
Source: Veterinary Research Communications - January 28, 2019 Category: Veterinary Research Source Type: research

Stem cells on regenerative and reproductive science in domestic animals
AbstractStem cells are undifferentiated and self-renewable cells that present new possibilities for both regenerative medicine and the understanding of early mammalian development. Adult multipotent stem cells are already widely used worldwide in human and veterinary medicine, and their therapeutic signalling, particularly with respect to immunomodulation, and their trophic properties have been intensively studied. The derivation of embryonic stem cells (ESCs) from domestic species, however, has been challenging, and the poor results do not reflect the successes obtained in mouse and human experiments. More recently, the g...
Source: Veterinary Research Communications - January 17, 2019 Category: Veterinary Research Source Type: research

The influence of Dermanyssus gallinae and different lighting regimens on selected blood proteins, corticosterone levels and egg production in layer hens
AbstractEgg production in battery cage systems in commercial poultry farms promotes uncontrolled growth of poultry ectoparasiteDermanyssus gallinae. Intermittent lighting regimens provided a promising alternative for controllingD. gallinae invasions. The study analysed the influence ofD. gallinae invasions on selected blood protein fractions (albumin, α-, β-, γ-globulin), corticosterone levels and egg production in Hy-Line Brown layer hens exposed to two lighting regimens: A (16 L:8D) and B (intermittent (4 L:2D); L-light, D-dark). Blood samples were collected from a total of 48 hens (divided int...
Source: Veterinary Research Communications - January 5, 2019 Category: Veterinary Research Source Type: research

Acinetobacter baumannii – a neglected pathogen in veterinary and environmental health in Germany
AbstractThe emergence and global spread of drug resistantAcinetobacter (A.) baumannii is a cause of great concern. The current knowledge on antibiotic resistance inA. baumannii from animal origin is mostly based on few internationally published case reports, investigations of strain collections and several whole genome analyses. This lack of data results in a somewhat sketchy picture on how to assess the possible impact of drug resistantA. baumannii strains on veterinary and public health in Germany. Consequently, there is an urgent need to intensify the surveillance ofA. baumannii in pet animals, the farm animal populatio...
Source: Veterinary Research Communications - December 27, 2018 Category: Veterinary Research Source Type: research

Impacts on performance of growing-finishing pigs under heat stress conditions: a meta-analysis
In conclusion, high ambient temperatures negatively influence performance parameters of growing-finishing pigs when compared with those in thermoneutral conditions. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - December 19, 2018 Category: Veterinary Research Source Type: research

Correction to: Optimised isolation method for RNA extraction suitable for RNA sequencing from feline teeth collected in a clinical setting and at post mortem
The authors regret that the original version of the above article contained errors in the Figs.  3, 4 and Tables 3 legends. The errors has been corrected. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - November 27, 2018 Category: Veterinary Research Source Type: research

Optimised isolation method for RNA extraction suitable for RNA sequencing from feline teeth collected in a clinical setting and at post mortem
AbstractAdvanced next generation sequencing approaches have started to reveal the cellular and molecular complexity of the microenvironment in many tissues. It is challenging to obtain high quality RNA from mineralised tissues. We developed an optimised method of RNA extraction from feline teeth collected in a clinical setting and at post mortem. Teeth were homogenised in phenol-guanidinium solution at near-freezing temperatures and followed by solid-phase nucleic acid extraction utilising a commercially available kit. This method produced good RNA yields and improved RNA quality based on RNA integrity numbers equivalent (...
Source: Veterinary Research Communications - November 6, 2018 Category: Veterinary Research Source Type: research

Could hypoxia influence basic biological properties and ultrastructural features of adult canine mesenchymal stem /stromal cells?
AbstractThe aim of the present study was to compare canine adipose tissue mesenchymal stem cells cultured under normoxic (20% O2) and not severe hypoxic (7% O2) conditions in terms of marker expression, proliferation rate, differentiation potential and cell morphology. Intra-abdominal fat tissue samples were recovered from 4 dogs and cells isolated from each sample were cultured under hypoxic and normoxic conditions. Proliferation rate and adhesion ability were determined, differentiation towards chondrogenic, osteogenic and adipogenic lineages was induced; the expression of CD44, CD34, DLA-DQA1, DLA-DRA1 was determined by...
Source: Veterinary Research Communications - September 20, 2018 Category: Veterinary Research Source Type: research

Isolation and characterization of two lytic bacteriophages against Staphylococcus aureus from India: newer therapeutic agents against Bovine mastitis
AbstractBovine mastitis causes severe economic losses to dairy farmers.Staphylococcus aureus, is one of the most important pathogen implicated in etiology of clinical and subclinical mastitis in bovines. In view of increasing antimicrobial resistance alternatives to antibiotic therapy are much needed. The present decade has witnessed a renewed interest in phage based therapeutics and diagnostics. The present study, describes isolation and characterization of two lytic phages SAJK-IND and MSP againstStaphylococcus aureus having a potential to be used in therapy against mastitis. SAJK-IND and MSP phages belonged toMyoviridae...
Source: Veterinary Research Communications - September 15, 2018 Category: Veterinary Research Source Type: research

Melanocortin 2 receptor antagonists in canine pituitary-dependent hypercortisolism: in vitro studies
In this study we investigated whether two peptide compounds, BIM-22776 (#776) and BIM-22A299 (#299), are effective MC2R antagonists in vitro. Their effects on cortisol production and mRNA expression of steroidogenic enzymes, MC2R and melanocortin 2 receptor accessory protein (MRAP) were evaluated in primary adrenocortical cell cultures (n = 8) of normal canine adrenal glands. Cortisol production stimulated by 50 nM ACTH was dose-dependently inhibited by #299 (inhibition 90.7 ± 2.3% at 5 μM) and by #776 (inhibition 38.0 ± 5.2% at 5 μM). The ACTH-st...
Source: Veterinary Research Communications - September 5, 2018 Category: Veterinary Research Source Type: research

Antibiotic susceptibility of Staphylococcus aureus isolated from subclinical bovine mastitis cases and in vitro efficacy of bacteriophage
AbstractStaphylococcus aureus is an opportunistic pathogen that may cause severe infections in livestock, and represents the major cause of mastitis in dairy cows. Currently, instead of using antibiotics, new strategies are sought to reduce this clinical health problem. The aim of this study was to determine the efficacy of phage therapy to killS. aureus strains obtained from farms located at the State of Guanajuato, M éxico. Thirty-sixS. aureus strains from cow milk with subclinical mastitis were isolated and identified, and the susceptibility to antibiotics and four phages also isolated in this work was tested. It...
Source: Veterinary Research Communications - September 1, 2018 Category: Veterinary Research Source Type: research

Effects of three-dimensional spheroid culture on equine mesenchymal stem cell plasticity
AbstractMesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy fields. We optimize culture conditions of equine adipose tissue-derived MSCs (eAD-MSCs) for treatment of horse fractures. To investigate enhancing properties of three-dimensional (3D) culture system in eAD-MSCs, we performed various sized spheroid formation and determined changes in gene expression levels to obtain different sized spheroid for cell therapy. eAD-MSCs were successfully isolated from horse tailhead. Using hanging drop method, spheroid formation was generated for three days. Quantitative real-time PCR was perfor...
Source: Veterinary Research Communications - September 1, 2018 Category: Veterinary Research Source Type: research

A comparison of the effects of antibiotics, probiotics, synbiotics and prebiotics on the performance and carcass characteristics of broilers
AbstractRoutine use of the antibiotic flavomycin in broiler production may lead to resistance, and alternative growth promoters are used to enhance performance. Two hundred day-old male Ross 308 broiler chicks were allocated to five dietary supplements included from d 1 –42: flavomycin, three possible alternatives, a probiotic, prebiotic and a synbiotic, as well as a control treatment. There were four replicate cages of 10 birds each in each treatment. Compared with the control and antibiotics treatments, the probiotic, prebiotic and synbiotic treatments increase d (p = 0.001) weight gain (64, 66, 73, 7...
Source: Veterinary Research Communications - September 1, 2018 Category: Veterinary Research Source Type: research

‘Nano-immuno test’ for the detection of live Mycobacterium avium subspecies paratuberculosis bacilli in the milk samples using magnetic nano-particles and chromogen
We report a simple, sensitive and specific nano-technology based ‘Nano-immuno test’ capable of detecting viable MAP bacilli in the milk samples within 10 h. Viable MAP bacilli were captured by MAP specific antibody-conjugated magnetic nano-particles using resazurin dye as chromogen. Test was optimized using true culture positive (10-bovine and 12-goats) and true culture negative (16-bovine and 25-goats) raw milk samples. Domestic livestock species in India are endemically infected with MAP. After successful optimization, sensitivity and specificity of the ‘nano-immuno test’ in goats with respec...
Source: Veterinary Research Communications - September 1, 2018 Category: Veterinary Research Source Type: research

Identification of bacteria present in ulcerative stomatitis lesions of captive sea turtles Chelonia mydas
AbstractAnthropogenic activities, predation, and diseases have contributed to a decrease in the sea turtle population in recent years. Ulcerative stomatitis is a condition that occurs in both wild and captive populations. The etiology of this condition is associated with bacteria such asE. coli,Citrobacter diversus,Klebsiella spp.,Pseudomonas spp.,Flavobacter calcoaceticus,Staphylococcus spp., andFlavobacterium spp. Some of these microorganisms are part of the oral microbiota of turtles, but alterations in the immune response can disturb the homeostatic relationship and cause an increase in the population of microorganisms...
Source: Veterinary Research Communications - September 1, 2018 Category: Veterinary Research Source Type: research

The synergistic effect of organic acids, phytochemicals and a permeabilizing complex reduces Salmonella Typhimurium 1,4,[5],12:i-shedding in pigs
AbstractSalmonella Typhimurium (includingS.Typhimurium 1,4,[5],12:i-) and other enteric pathogens cause acute infection in pigs during the weaning stage, often evolving into chronic infections responsible for the introduction of zoonotic bacteria into the slaughterhouse and thus determining carcass contamination. In addition to being zoonotic hazards, these pathogens are responsible for economic losses in affected farms. Traditionally, antibiotic treatments have been largely administered in order to reduce the infection burden but it favored, as a direct consequence, an increase in the number of multi-drug resistance strai...
Source: Veterinary Research Communications - September 1, 2018 Category: Veterinary Research Source Type: research

The effect of ammonia on canine polymorphonuclear cells
AbstractHyperammonaemia is a common complication of liver disease in dogs. High concentrations of ammonia can be detrimental to dogs with liver disease for several reasons, notably by causing hepatic encephalopathy (HE) which describes the wide range of neurological abnormalities ranging from altered behaviour to seizures that are well recognised complications in dogs with hepatic disorders. In human patients with liver disease, hyperammonaemia has also been linked to the development of other systemic complications such as dysregulation of the innate immune system. In contrast, the effects of hyperammonaemia on the canine ...
Source: Veterinary Research Communications - September 1, 2018 Category: Veterinary Research Source Type: research

Doxycycline treatment for Dirofilaria immitis in dogs: impact on Staphylococcus aureus and Enterococcus  antimicrobial resistance
In this study, 17 heartworm-infected dogs (10 that had completed the doxycycline treatment and 7 dogs that had not yet begun) were included. Twenty-four isolates ofStaphylococcus aureus were obtained from two locations of each dog. After treatment, 73.3% of isolates were resistant to at least one antibiotic but only 22.2% of isolates before treatment. Most of doxycycline resistant isolates were obtained from dogs that have received treatment. Erythromycin resistance or intermediate susceptibility was detected in 45.6% of isolates, most of them from dogs after treatment. For Enterococci, 48 isolates were obtained from fecal...
Source: Veterinary Research Communications - September 1, 2018 Category: Veterinary Research Source Type: research

A porcine-cholecyst-derived scaffold for treating full thickness lacerated skin wounds in dogs
AbstractIn regenerative medicine, despite the chances of graft-rejection, scaffolds prepared from extracellular matrices of various mammalian organs/tissues are widely used. Graft-assisted healing of full thickness skin-wounds is a major use of these bioscaffolds. Therefore, considering its prospective clinical use as a wound healing matrix, this study evaluated the healing potential of porcine cholecyst-derived scaffold (CDS) prepared by a non-detergent/enzymatic method for treating naturally occurring full thickness lacerated wounds in dogs. The CDS caused, in comparison with a commercial-grade bioscaffold prepared out o...
Source: Veterinary Research Communications - September 1, 2018 Category: Veterinary Research Source Type: research

Correction to: The synergistic effect of organic acids, phytochemicals and a permeabilizing complex reduces Salmonella Typhimurium 1,4,[5],12:i-shedding in pigs
This article was originally published with all author names incorrectly listed. All author names have now been transposed and appear correctly above. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - September 1, 2018 Category: Veterinary Research Source Type: research

Cell free mitochondrial DNA in serum and milk associated with bovine mastitis: a pilot study
AbstractMastitis is inflammation of mammary gland affecting all the species of domestic animals. Fragments of the mitochondrial genome released from dying cells are considered surrogate markers of mitochondrial injury. We hypothesized that bovine mastitis would be associated with increased cell free mitochondrial DNA (mtDNA) content in serum and milk. Milk and serum samples were collected from sub-clinical mastitic and normal animals. Mastitis was confirmed by California mastitis test and bacterial isolation. Oxidative stress, nitric oxide and inflammatory cytokines were also estimated. Real time polymerase chain reaction ...
Source: Veterinary Research Communications - August 25, 2018 Category: Veterinary Research Source Type: research

Cervico-vaginal mucus (CVM) – an accessible source of immunologically informative biomolecules
AbstractCervico-vaginal mucus (CVM), the product of epithelial cells lining the uterus, cervix and vagina, is secreted to facilitate uterine lubrication and microbial clearance. Predominantly composed of water and mucins, CVM also contains high levels of immuno-active proteins such as immunoglobulin A (IgA), lactoferrin and lysozyme which protect against infection by blocking adhesion and mediating microbial killing. The repertoire of cytokines, chemokines and antimicrobial peptides is predominantly generated by the secretions of endometrial epithelial cells into the uterine lumen and concentrated in the CVM. The quantity ...
Source: Veterinary Research Communications - August 16, 2018 Category: Veterinary Research Source Type: research

Triggers of NLRC4 and AIM2 inflammasomes induce porcine IL-1 β secretion
In this study, we treated well defined NLRC4 and AIM2 inflammasome triggers to porcine peripheral blood mononuclear cells (PBMCs) and murine bone-marrow derived macrophages (BMDMs) and observed interleukin (IL)-1 β maturation as a readout of inflammasome activation. NLRC4 (flagellin) and AIM2 (dsDNA) triggers led to IL-1β secretion in both porcine PBMCs and mice macrophages. In addition, porcine and mouse NLRC4 and AIM2 inflammasomes responded differently to NLRP3 inhibitors. Bacterial inflammasome trigger s,Salmonella enterica serovar Typhimurium,Listeria monocytogenes, andEscherichia coli, also induced IL-1 &be...
Source: Veterinary Research Communications - August 10, 2018 Category: Veterinary Research Source Type: research

The IMPDH inhibitors, ribavirin and mycophenolic acid, inhibit peste des petits ruminants virus infection
(Source: Veterinary Research Communications)
Source: Veterinary Research Communications - August 9, 2018 Category: Veterinary Research Source Type: research

Correction to: The synergistic effect of organic acids, phytochemicals and a permeabilizing complex reduces Salmonella Typhimurium 1,4,[5],12:i-shedding in pigs
This article was originally published with all author names incorrectly listed. All author names have now been transposed and appear correctly above. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - August 6, 2018 Category: Veterinary Research Source Type: research

A porcine-cholecyst-derived scaffold for treating full thickness lacerated skin wounds in dogs
AbstractIn regenerative medicine, despite the chances of graft-rejection, scaffolds prepared from extracellular matrices of various mammalian organs/tissues are widely used. Graft-assisted healing of full thickness skin-wounds is a major use of these bioscaffolds. Therefore, considering its prospective clinical use as a wound healing matrix, this study evaluated the healing potential of porcine cholecyst-derived scaffold (CDS) prepared by a non-detergent/enzymatic method for treating naturally occurring full thickness lacerated wounds in dogs. The CDS caused, in comparison with a commercial-grade bioscaffold prepared out o...
Source: Veterinary Research Communications - August 1, 2018 Category: Veterinary Research Source Type: research

Antibiotic susceptibility of Staphylococcus aureus isolated from subclinical bovine mastitis cases and in vitro efficacy of bacteriophage
AbstractStaphylococcus aureus is an opportunistic pathogen that may cause severe infections in livestock, and represents the major cause of mastitis in dairy cows. Currently, instead of using antibiotics, new strategies are sought to reduce this clinical health problem. The aim of this study was to determine the efficacy of phage therapy to killS. aureus strains obtained from farms located at the State of Guanajuato, M éxico. Thirty-sixS. aureus strains from cow milk with subclinical mastitis were isolated and identified, and the susceptibility to antibiotics and four phages also isolated in this work was tested. It...
Source: Veterinary Research Communications - July 24, 2018 Category: Veterinary Research Source Type: research

The effect of ammonia on canine polymorphonuclear cells
AbstractHyperammonaemia is a common complication of liver disease in dogs. High concentrations of ammonia can be detrimental to dogs with liver disease for several reasons, notably by causing hepatic encephalopathy (HE) which describes the wide range of neurological abnormalities ranging from altered behaviour to seizures that are well recognised complications in dogs with hepatic disorders. In human patients with liver disease, hyperammonaemia has also been linked to the development of other systemic complications such as dysregulation of the innate immune system. In contrast, the effects of hyperammonaemia on the canine ...
Source: Veterinary Research Communications - June 24, 2018 Category: Veterinary Research Source Type: research

Doxycycline treatment for Dirofilaria immitis in dogs: impact on Staphylococcus aureus and Enterococcus  antimicrobial resistance
In this study, 17 heartworm-infected dogs (10 that had completed the doxycycline treatment and 7 dogs that had not yet begun) were included. Twenty-four isolates ofStaphylococcus aureus were obtained from two locations of each dog. After treatment, 73.3% of isolates were resistant to at least one antibiotic but only 22.2% of isolates before treatment. Most of doxycycline resistant isolates were obtained from dogs that have received treatment. Erythromycin resistance or intermediate susceptibility was detected in 45.6% of isolates, most of them from dogs after treatment. For Enterococci, 48 isolates were obtained from fecal...
Source: Veterinary Research Communications - June 24, 2018 Category: Veterinary Research Source Type: research

Identification of bacteria present in ulcerative stomatitis lesions of captive sea turtles Chelonia mydas
AbstractAnthropogenic activities, predation, and diseases have contributed to a decrease in the sea turtle population in recent years. Ulcerative stomatitis is a condition that occurs in both wild and captive populations. The etiology of this condition is associated with bacteria such asE. coli,Citrobacter diversus,Klebsiella spp.,Pseudomonas spp.,Flavobacter calcoaceticus,Staphylococcus spp., andFlavobacterium spp. Some of these microorganisms are part of the oral microbiota of turtles, but alterations in the immune response can disturb the homeostatic relationship and cause an increase in the population of microorganisms...
Source: Veterinary Research Communications - June 22, 2018 Category: Veterinary Research Source Type: research

Effectiveness of different avian influenza (H5) vaccination regimens in layer chickens on the humoral immune response and interferon-alpha signalling immune marker
AbstractAvian influenza (AI) vaccines are widely used to control and eliminate the ongoing avian influenza virus epidemic in Egypt. A strict vaccination policy with inactivated AI vaccines has been widely applied, however the virus still circulating, evolving and causing great negative impact to the poultry sector in Egypt. Therefore, an updated poultry vaccination policy using different vaccine technologies might be valuable as an innovative additional control strategy of AIV in Egypt. In the present study, the effectiveness of different avian influenza (AI) vaccination schedules was evaluated in 300 commercial layer chic...
Source: Veterinary Research Communications - June 1, 2018 Category: Veterinary Research Source Type: research

A long term feed supplementation based on phosphate binders in Feline Chronic Kidney Disease
AbstractChronic kidney disease (CKD) is a very common disorder in elderly cats. A proper renal diet represents the most efficient therapeutic intervention to improve survival and life quality in feline patients with 3 and 4 International Renal Interest Society (IRIS) stages. Twenty cats were selected in this study. Ten were administered the dietary supplementation for 360 days and the other ten, whose owners did not give consent for any supplemental therapies apart from the renal diet, were selected from a clinical database and used as control group. The present study is a long term study (360 days) aiming to evaluate the ...
Source: Veterinary Research Communications - June 1, 2018 Category: Veterinary Research Source Type: research