Viruses, Vol. 16, Pages 687: Development of Attenuated Viruses for Effective Protection against Pepper Veinal Mottle Virus in Tomato Crops
In this study, we performed nitrite-induced mutagenesis of the PVMV tomato isolate Tn to generate attenuated PVMV mutants. PVMV Tn causes necrotic lesions in Chenopodium quinoa leaves and severe mosaic and wilting in Nicotiana benthamiana plants. After nitrite treatment, three attenuated PVMV mutants, m4-8, m10-1, and m10-11, were selected while inducing milder responses to C. quinoa and N. benthamiana with lower accumulation in tomato plants. In greenhouse tests, the three mutants showed different degrees of cross-protection against wild-type PVMV Tn. m4-8 showed the highest protective efficacy against PVMV Tn in N. benth...
Source: Viruses - April 26, 2024 Category: Virology Authors: Guan-Da Wang Chian-Chi Lin Tsung-Chi Chen Tags: Article Source Type: research
Viruses, Vol. 16, Pages 688: Long-Term Impairment of Working Ability in Subjects under 60 Years of Age Hospitalised for COVID-19 at 2 Years of Follow-Up: A Cross-Sectional Study
Conclusions: Our findings suggest that PASC promotes conditions that could result in decreased working ability and unemployment. These results highlight the significant impact of this syndrome on public health and the global economy, and the need to develop clinical pathways and guidelines for long-term care with specific focus on working impairment. (Source: Viruses)
Source: Viruses - April 26, 2024 Category: Virology Authors: Luisa Frallonardo Annunziata Ilenia Ritacco Angela Amendolara Domenica Cassano Giorgia Manco Cesari Alessia Lugli Mariangela Cormio Michele De Filippis Greta Romita Giacomo Guido Luigi Piccolomo Vincenzo Giliberti Francesco Cavallin Francesco Vladimiro Se Tags: Article Source Type: research
Viruses, Vol. 16, Pages 690: Challenges in Treating Pediatric Cancer Patients during the COVID-19 Pandemic: Balancing Risks and Care
es-Espíndola
The COVID-19 pandemic has resulted in millions of fatalities worldwide. The case of pediatric cancer patients stands out since, despite being considered a population at risk, few studies have been carried out concerning symptom detection or the description of the mechanisms capable of modifying the course of the COVID-19 disease, such as the interaction and response between the virus and the treatment given to cancer patients. By synthesizing existing studies, this paper aims to expose the treatment challenges for pediatric patients with COVID-19 in an oncology context. Additionally, this updated review i...
Source: Viruses - April 26, 2024 Category: Virology Authors: Ch ávez-Pacheco Castillejos-L ópez Laura M. Hern ández-Regino Velasco-Hidalgo Zapata-Tarres Correa-Carranza Rosario-M éndez Barrientos-R íos Aquino-G álvez Torres-Esp índola Tags: Review Source Type: research
Viruses, Vol. 16, Pages 689: Deletion of 82 & ndash;85 N-Terminal Residues in SARS-CoV-2 Nsp1 Restricts Virus Replication
Viruses, Vol. 16, Pages 689: Deletion of 82–85 N-Terminal Residues in SARS-CoV-2 Nsp1 Restricts Virus Replication
Viruses doi: 10.3390/v16050689
Authors:
Gianni Gori Savellini
Gabriele Anichini
Fabrizio Manetti
Claudia Immacolata Trivisani
Maria Grazia Cusi
Non-structural protein 1 (Nsp1) represents one of the most crucial SARS-CoV-2 virulence factors by inhibiting the translation of host mRNAs and promoting their degradation. We selected naturally occurring virus lineages with specific Nsp1 deletions located at both the N- and C-terminus of the protein. Our data provide new insights into how Nsp1...
Source: Viruses - April 26, 2024 Category: Virology Authors: Gianni Gori Savellini Gabriele Anichini Fabrizio Manetti Claudia Immacolata Trivisani Maria Grazia Cusi Tags: Article Source Type: research
Viruses, Vol. 16, Pages 691: Diagnostic Utility of Cytomegalovirus (CMV) DNA Quantitation in Ulcerative Colitis
This study aimed to investigate the diagnostic value of the presence of CMV DNA in intestinal mucosa tissue and blood samples in patients with active UC. This study included 81 patients with exacerbated symptoms of UC. Patient data were obtained from the Hospital Information Management System. CMV DNA in colorectal tissue and plasma samples were analyzed using a real-time quantitative PCR assay. CMV markers were detected using immunohistochemistry and hematoxylin–eosin staining. Immunohistochemistry positivity was observed in tissue samples from eight (9.9%) patients. Only one (1.2%) patient showed CMV-specif...
Source: Viruses - April 26, 2024 Category: Virology Authors: Sema Esen Imran Saglik Enver Dolar Selcan Cesur Nesrin Ugras Harun Agca Osman Merdan Beyza Ener Tags: Article Source Type: research
Viruses, Vol. 16, Pages 680: Human Papillomavirus and Associated Cancers: A Review
Rysavy
The human papillomavirus is the most common sexually transmitted infection in the world. Most HPV infections clear spontaneously within 2 years of infection; however, persistent infection can result in a wide array of diseases, ranging from genital warts to cancer. Most cases of cervical, anal, and oropharyngeal cancers are due to HPV infection, with cervical cancer being one of the leading causes of cancer death in women worldwide. Screening is available for HPV and cervical cancer, but is not available everywhere, particularly in lower-resource settings. HPV infection disproportionally affects individuals liv...
Source: Viruses - April 26, 2024 Category: Virology Authors: JaNiese E. Jensen Greta L. Becker J. Brooks Jackson Mary B. Rysavy Tags: Review Source Type: research
Viruses, Vol. 16, Pages 679: T Cell Surveillance during Cutaneous Viral Infections
man
The skin is a complex tissue that provides a strong physical barrier against invading pathogens. Despite this, many viruses can access the skin and successfully replicate in either the epidermal keratinocytes or dermal immune cells. In this review, we provide an overview of the antiviral T cell biology responding to cutaneous viral infections and how these responses differ depending on the cellular targets of infection. Much of our mechanistic understanding of T cell surveillance of cutaneous infection has been gained from murine models of poxvirus and herpesvirus infection. However, we also discuss other viral inf...
Source: Viruses - April 26, 2024 Category: Virology Authors: Luxin Pei Heather D. Hickman Tags: Review Source Type: research
Viruses, Vol. 16, Pages 681: Monkeypox Virus Neutralizing Antibodies at Six Months from Mpox Infection: Virologic Factors Associated with Poor Immunologic Response
This study found that the overall days of monkeypox virus detection in the body, irrespective of the viral loads, were directly correlated with monkeypox virus neutralizing antibodies at six months after infection. (Source: Viruses)
Source: Viruses - April 26, 2024 Category: Virology Authors: Angelo Roberto Raccagni Alessandro Mancon Sara Diotallevi Riccardo Lolatto Elena Bruzzesi Maria Rita Gismondo Antonella Castagna Davide Mileto Silvia Nozza Tags: Article Source Type: research
Viruses, Vol. 16, Pages 683: Evolution Characterization and Pathogenicity of an NADC34-like PRRSV Isolated from Inner Mongolia, China
This report can provide a basis for epidemiological investigations and subsequent studies of PRRSV. (Source: Viruses)
Source: Viruses - April 26, 2024 Category: Virology Authors: Hong-Zhe Zhao Chun-Yu Liu Hai Meng Cheng-Long Sun Hong-Wen Yang Hao Wang Jian Zou Peng Li Feng-Ye Han Gen Qi Yang Zhang Bing-Bing Lin Chuang Liu Meng-Meng Chen Pan-Ling Zhang Xiao-Dong Chen Yi-Di Zhang Qian-Jin Song Yong-Jun Wen Feng-Xue Wang Tags: Article Source Type: research
Viruses, Vol. 16, Pages 682: Reduction of the Risk of Hepatocellular Carcinoma over Time Using Direct-Acting Antivirals: A Propensity Score Analysis of a Real-Life Cohort (PITER HCV)
unetto
Anna Linda Zignego
Carmine Coppola
Andrea Iannone
Elisa Biliotti
Elena Rosselli Del Turco
Marco Massari
Anna Licata
Francesco Barbaro
Marcello Persico
Filomena Morisco
Maurizio Pompili
Federica Cerini
Massimo Puoti
Teresa Santantonio
Antonio Craxì
Loreta A. Kondili
Liliana Chemello Investigators
on behalf of PITER Collaborating Investigators on behalf of PITER Collaborating Investigators
The treatment of hepatitis C virus (HCV) with direct-acting antivirals (DAA) leads to high sustained virological response (SVR) rates, but hepatocellular carcinoma (HCC) risk persists in peop...
Source: Viruses - April 26, 2024 Category: Virology Authors: Maria Giovanna Quaranta Luisa Cavalletto Francesco Paolo Russo Vincenza Calvaruso Luigina Ferrigno Alberto Zanetto Benedetta Mattioli Roberta D ’Ambrosio Valentina Panetta Giuseppina Brancaccio Giovanni Raimondo Maurizia Rossana Brunetto Anna Linda Zign Tags: Article Source Type: research
Viruses, Vol. 16, Pages 685: Impact of Prior COVID-19 Immunization and/or Prior Infection on Immune Responses and Clinical Outcomes
oglou
Cellular and humoral immunity exhibit dynamic adaptation to the mutating SARS-CoV-2 virus. It is noteworthy that immune responses differ significantly, influenced by whether a patient has received vaccination or whether there is co-occurrence of naturally acquired and vaccine-induced immunity, known as hybrid immunity. The different immune reactions, conditional on vaccination status and the viral variant involved, bear implications for inflammatory responses, patient outcomes, pathogen transmission rates, and lingering post-COVID conditions. Considering these developments, we have performed a review of recently ...
Source: Viruses - April 26, 2024 Category: Virology Authors: Achilleas Livieratos Charalambos Gogos Karolina Akinosoglou Tags: Review Source Type: research
Viruses, Vol. 16, Pages 686: Unraveling the Properties of Phage Display Fab Libraries and Their Use in the Selection of Gliadin-Specific Probes by Applying High-Throughput Nanopore Sequencing
rcía
Directed evolution is a pivotal strategy for new antibody discovery, which allowed the generation of high-affinity Fabs against gliadin from two antibody libraries in our previous studies. One of the libraries was exclusively derived from celiac patients’ mRNA (immune library) while the other was obtained through a protein engineering approach (semi-immune library). Recent advances in high-throughput DNA sequencing techniques are revolutionizing research across genomics, epigenomics, and transcriptomics. In the present work, an Oxford Nanopore in-lab sequencing device was used to comprehensively cha...
Source: Viruses - April 26, 2024 Category: Virology Authors: Eduardo Garcia-Calvo Aina Garc ía-García Santiago Rodr íguez Rosario Mart ín Teresa Garc ía Tags: Article Source Type: research
Viruses, Vol. 16, Pages 684: Hepatitis E Virus in Domestic Ruminants and Virus Excretion in Milk & mdash;A Potential Source of Zoonotic HEV Infection
Viruses, Vol. 16, Pages 684: Hepatitis E Virus in Domestic Ruminants and Virus Excretion in Milk—A Potential Source of Zoonotic HEV Infection
Viruses doi: 10.3390/v16050684
Authors:
Gergana Zahmanova
Katerina Takova
Georgi L. Lukov
Anton Andonov
The hepatitis E virus is a serious health concern worldwide, with 20 million cases each year. Growing numbers of autochthonous HEV infections in industrialized nations are brought on via the zoonotic transmission of HEV genotypes 3 and 4. Pigs and wild boars are the main animal reservoirs of HEV and play the primary role in HEV transmission. Consumption of r...
Source: Viruses - April 26, 2024 Category: Virology Authors: Gergana Zahmanova Katerina Takova Georgi L. Lukov Anton Andonov Tags: Review Source Type: research
Viruses, Vol. 16, Pages 671: Analytical and Clinical Performance of the NeuMoDx & trade; Platform for Cytomegalovirus and Epstein & ndash;Barr Virus Viral Load Testing
In conclusion, NeuMoDx CMV and EBV Quant Assays are sensitive and accurate tools for CMV and EBV DNA VL quantification. (Source: Viruses)
Source: Viruses - April 25, 2024 Category: Virology Authors: Lindsay Coupland Katy Woodward Samir Dervisevic Rachel Hale Stephen Brolly Tags: Brief Report Source Type: research
Viruses, Vol. 16, Pages 672: Longitudinal Dynamics of Immune Response in Occupational Populations Post COVID-19 Infection in the Changning District of Shanghai, China
Yihan Lu
Jianlin Zhuang
Monitoring the long-term changes in antibody and cellular immunity following Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection is crucial for understanding immune mechanisms that prevent reinfection. In March 2023, we recruited 167 participants from the Changning District, Shanghai, China. A subset of 66 participants that were infected between November 2022 and January 2023 was selected for longitudinal follow-up. The study aimed to investigate the dynamics of the immune response, including neutralizing antibodies (NAbs), anti-spike (S)-immunoglobulin G (IgG), anti-S-IgM, ...
Source: Viruses - April 25, 2024 Category: Virology Authors: Li Li Fengge Wang Xiaoding He Tingting Pei Jiani Lu Zhan Zhang Ping Zhao Jiayu Xue Lin Zhu Xinxin Chen Zijie Yan Yihan Lu Jianlin Zhuang Tags: Article Source Type: research
