Viruses, Vol. 16, Pages 400: Using an In Vivo Mouse Model to Determine the Exclusion Criteria of Preexisting Anti-AAV9 Neutralizing Antibody Titer of Pompe Disease Patients in Clinical Trials
g Wu
Xiaoyan Dong
The efficacy of adeno-associated virus (AAV)-based gene therapy is dependent on effective viral transduction, which might be inhibited by preexisting immunity to AAV acquired from infection or maternal delivery. Anti-AAV neutralizing Abs (NAbs) titer is usually measured by in vitro assay and used for patient enroll; however, this assay could not evaluate NAbs’ impacts on AAV pharmacology and potential harm in vivo. Here, we infused a mouse anti-AAV9 monoclonal antibody into Balb/C mice 2 h before receiving 1.2 × 1014 or 3 × 1013 vg/kg of rAAV9-coGAA by tail ...
Source: Viruses - March 5, 2024 Category: Virology Authors: Hanqing Wang Cengceng Zhang Zheyue Dong Xueyang Zhu Xuchu Zheng Ziyang Liu Jianfang Zhou Shuangqing Yu Xiaobing Wu Xiaoyan Dong Tags: Article Source Type: research
Viruses, Vol. 16, Pages 401: Difference in Intraspecies Transmissibility of Severe Fever with Thrombocytopenia Syndrome Virus Depending on Abrogating Type 1 Interferon Signaling in Mice
Oh
Bumseok Kim
Severe fever with thrombocytopenia syndrome (SFTS), a tick-borne zoonotic disease, is caused by infection with SFTS virus (SFTSV). A previous study reported that human-to-human direct transmission of SFTSV can occur. However, potential animal-to-animal transmission of SFTSV without ticks has not been fully clarified. Thus, the objective of this study was to investigate potential mice-to-mice transmission of SFTSV by co-housing three groups of mice [i.e., wild-type mice (WT), mice injected with an anti-type I interferon-α receptor-blocking antibody (IFNAR Ab), and mice with knockout of ty...
Source: Viruses - March 5, 2024 Category: Virology Authors: Byungkwan Oh Seok-Chan Park Myeon-Sik Yang Daram Yang Gaeul Ham Dongseob Tark Myung Jo You Sang-Ik Oh Bumseok Kim Tags: Article Source Type: research
Viruses, Vol. 16, Pages 397: Correction: Cook et al. An Optimized Bioassay for Screening Combined Anticoronaviral Compounds for Efficacy against Feline Infectious Peritonitis Virus with Pharmacokinetic Analyses of GS-441524, Remdesivir, and Molnupiravir in Cats. Viruses 2022, 14, 2429
ler
Brian G. Murphy
In the original publication [...] (Source: Viruses)
Source: Viruses - March 4, 2024 Category: Virology Authors: Sarah Cook Luke Wittenburg Victoria C. Yan Jacob H. Theil Diego Castillo Krystle L. Reagan Sonyia Williams Cong-Dat Pham Chun Li Florian L. Muller Brian G. Murphy Tags: Correction Source Type: research
Viruses, Vol. 16, Pages 398: Evolution of Endogenous Retroviruses in the Subfamily of Caprinae
In this study, we used multiple pipelines to conduct a de novo exploration and annotation of ERVs in 13 species of the Caprinae subfamily. Through analyses of sequence identity, structural organization, and phylogeny, we defined 28 ERV groups within Caprinae, including 19 gamma retrovirus groups and 9 beta retrovirus groups. Notably, we identified four recent and potentially active groups prevalent in the Caprinae genomes. Additionally, our investigation revealed that most long noncoding genes (lncRNA) and protein-coding genes (PC) contain ERV-derived sequences. Specifically, we observed that ERV-derived sequences were pre...
Source: Viruses - March 4, 2024 Category: Virology Authors: Ali Shoaib Moawad Fengxu Wang Yao Zheng Cai Chen Ahmed A. Saleh Jian Hou Chengyi Song Tags: Article Source Type: research
Viruses, Vol. 16, Pages 399: Prevalence of Emergent Dolutegravir Resistance Mutations in People Living with HIV: A Rapid Scoping Review
Conclusions: In clinical trials, the prevalence of VF with emergent INSTI DRMs in PLWH receiving DTG-containing regimens has been low. Novel approaches are required to assess VF prevalence with emergent INSTI DRMs in PLWH receiving DTG in real-world settings. (Source: Viruses)
Source: Viruses - March 4, 2024 Category: Virology Authors: Carolyn Chu Kaiming Tao Vinie Kouamou Ava Avalos Jake Scott Philip M. Grant Soo-Yon Rhee Suzanne M. McCluskey Michael R. Jordan Rebecca L. Morgan Robert W. Shafer Tags: Review Source Type: research
Viruses, Vol. 16, Pages 396: The Multi-Kingdom Microbiome of Wintering Migratory Birds in Poyang Lake, China
Dayan Wang
Wild birds are a natural reservoir for zoonotic viruses. To clarify the role of migratory birds in viruses spread in Poyang Lake, we investigated the microbiome of 250 wild bird samples from 19 species in seven orders. The bacterial and viral content abundance and diversity were preliminarily evaluated by Kraken2 and Bracken. After de novo assembly by Megahit and Vamb, viral contigs were identified by CheckV. The reads remapped to viral contigs were quantified using Bowtie2. The bacterial microbiome composition of the samples covers 1526 genera belonging to 175 bacterial orders, while the composition of v...
Source: Viruses - March 3, 2024 Category: Virology Authors: Jia Liu Xiyan Li Wentao Song Xiaoxu Zeng Hui Li Lei Yang Dayan Wang Tags: Article Source Type: research
Viruses, Vol. 16, Pages 395: Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America
h
Aguiar
Traub-Cseko
Sandflies are known vectors of leishmaniasis. In the Old World, sandflies are also vectors of viruses while little is known about the capacity of New World insects to transmit viruses to humans. Here, we relate the identification of RNA sequences with homology to rhabdovirus nucleocapsids (NcPs) genes, initially in the Lutzomyia longipalpis LL5 cell lineage, named NcP1.1 and NcP2. The Rhabdoviridae family never retrotranscribes its RNA genome to DNA. The sequences here described were identified in cDNA and DNA from LL-5 cells and in adult insects indicating that they are transcribed endogenou...
Source: Viruses - March 2, 2024 Category: Virology Authors: Tempone Zezza-Ramalho Borely Pitaluga Brazil Brand ão-Filho Pessoa Bruno Carvalho-Costa Salom ón Volf Burleigh Aguiar Traub-Cseko Tags: Article Source Type: research
Viruses, Vol. 16, Pages 393: Quantification of Hepatitis E Virus ORF2 Protein by a Novel Sandwich ELISA
ivern
Hepatitis E virus (HEV) causes acute hepatitis in humans, which can progress to chronicity in immunosuppressed individuals. Almost all reported HEV infections are caused by Paslahepevirus balayani genotypes 1–4. The structural ORF2 protein is the major antigen detected in the blood of HEV-infected individuals. ELISA assays to detect IgM antibodies to HEV are the first-line diagnostic tests; however, they showed variable performance with frequently discordant results. A qualitative HEV antigen (ORF2) ELISA is currently available for research use. Here, we report a novel quantitative sand...
Source: Viruses - March 2, 2024 Category: Virology Authors: Sakthivel Subramaniam Rafaelle Fares-Gusmao David R. McGivern Tags: Article Source Type: research
Viruses, Vol. 16, Pages 394: Prospective Investigation of Feline Leukemia Virus Infection in Stray Cats Subjected to a Trap & ndash;Neuter & ndash;Return Program in Switzerland
Viruses, Vol. 16, Pages 394: Prospective Investigation of Feline Leukemia Virus Infection in Stray Cats Subjected to a Trap–Neuter–Return Program in Switzerland
Viruses doi: 10.3390/v16030394
Authors:
Marina L. Meli
Benita Pineroli
Esther Geisser
Regina Hofmann-Lehmann
Feline leukemia virus (FeLV) remains a serious concern in some countries despite advances in diagnostics and vaccines. FeLV-infected cats often have reduced lifespans due to FeLV-associated diseases. The infection is transmitted through social interactions. While Northern European countries have reported a decrease in FeLV a...
Source: Viruses - March 2, 2024 Category: Virology Authors: Marina L. Meli Benita Pineroli Esther Geisser Regina Hofmann-Lehmann Tags: Article Source Type: research
Viruses, Vol. 16, Pages 391: Differentiating Cell Entry Potentials of SARS-CoV-2 Omicron Subvariants on Human Lung Epithelium Cells
ucker
Maolin Lu
The surface spike (S) glycoprotein mediates cell entry of SARS-CoV-2 into the host through fusion at the plasma membrane or endocytosis. Omicron lineages/sublineages have acquired extensive mutations in S to gain transmissibility advantages and altered antigenicity. The fusogenicity, antigenicity, and evasion of Omicron subvariants have been extensively investigated at unprecedented speed to align with the mutation rate of S. Cells that overexpress receptors/cofactors are mostly used as hosts to amplify infection sensitivity to tested variants. However, systematic cell entry comparisons of most prior ...
Source: Viruses - March 1, 2024 Category: Virology Authors: Revansiddha H. Katte Yuanyun Ao Wang Xu Yang Han Guohua Zhong Dibya Ghimire Jon Florence Torry A. Tucker Maolin Lu Tags: Article Source Type: research
Viruses, Vol. 16, Pages 392: Characterization of the RNA Mycovirome Associated with Grapevine Fungal Pathogens: Analysis of Mycovirus Distribution and Their Genetic Variability within a Collection of Botryosphaeriaceae Isolates
In this study, the RNA mycovirome of fifteen Botryosphaeriaceae isolates was characterized through the high-throughput sequencing of double-stranded RNA preparations from the respective samples. Eight mycoviruses were detected, including three potential novel species in the Narnaviridae family, as well as in the proposed Mycobunyaviridae and Fusagraviridae families. A large collection of Botryosphaeriaceae isolates was screened using RT-PCR assays specific for 20 Botryosphaeriaceae-infecting mycoviruses. Among the mycoviruses detected, some appeared to be specialists within a single host species, while others infected isol...
Source: Viruses - March 1, 2024 Category: Virology Authors: Gwena ëlle Comont Chantal Faure Thierry Candresse Marie Laurens Sophie Vali ère J érôme Lluch Marie Lefebvre S ébastien Gambier J érôme Jolivet Marie-France Corio-Costet Armelle Marais Tags: Article Source Type: research
Viruses, Vol. 16, Pages 387: Novel Viral Vectors for Gene Therapy
r
Viral vectors are gene transfer tools assembled from the backbones of naturally occurring viruses [...] (Source: Viruses)
Source: Viruses - March 1, 2024 Category: Virology Authors: Ottmar Herchenr öder Brigitte M. P ützer Tags: Editorial Source Type: research
Viruses, Vol. 16, Pages 388: Impact of the H274Y Substitution on N1, N4, N5, and N8 Neuraminidase Enzymatic Properties and Expression in Reverse Genetic Influenza A Viruses
In conclusion, the H274Y-NA substitution of different group-1-NAs systematically reduced their affinity for MUNANA substrate without a significant impact on NA Vm. The impact of the H274Y-NA substitution on viral NA expression was different according to the studied NA. (Source: Viruses)
Source: Viruses - March 1, 2024 Category: Virology Authors: Alexandre Gaymard Caroline Picard Guilhem Vazzoler Pascale Massin Emilie Frobert Murielle Sabatier Mendy Barthelemy Martine Valette Mich èle Ottmann Jean-S ébastien Casalegno Bruno Lina Vanessa Escuret Tags: Communication Source Type: research
Viruses, Vol. 16, Pages 389: Ongoing Gaps in the Hepatitis C Care Cascade during the Direct-Acting Antiviral Era in a Large Retrospective Cohort in Canada: A Population-Based Study
Christina Greenaway
To achieve hepatitis C virus (HCV) elimination, high uptake along the care cascade steps for all will be necessary. We mapped engagement with the care cascade overall and among priority groups in the post-direct-acting antivirals (DAAs) period and assessed if this changed relative to pre-DAAs. We created a population-based cohort of all reported HCV diagnoses in Quebec (1990–2018) and constructed the care cascade [antibody diagnosed, RNA tested, RNA positive, genotyped, treated, sustained virologic response (SVR)] in 2013 and 2018. Characteristics associated with RNA testing and trea...
Source: Viruses - March 1, 2024 Category: Virology Authors: Ana Maria Passos-Castilho Donald G. Murphy Karine Blouin Andrea Benedetti Dimitra Panagiotoglou Julie Bruneau Marina B. Klein Jeffrey C. Kwong Beate Sander Naveed Z. Janjua Christina Greenaway Tags: Article Source Type: research
Viruses, Vol. 16, Pages 390: CD8+ T Cells Mediate Lethal Lung Pathology in the Absence of PD-L1 and Type I Interferon Signalling following LCMV Infection
In this study, utilizing single- and double-knockout mice, we aimed to determine whether ablation of PD-1 could restore T cell functionality in the absence of IFNAR1 signalling in LCMV-Arm-infected mice. Surprisingly, this did not re-invigorate the T cell response and instead, it converted chronic LCMV-Arm infection into a lethal disease characterized by severe lung inflammation with an infiltration of neutrophils and T cells. Depletion of CD8+ T cells, but not neutrophils, rescued mice from lethal disease, demonstrating that IFNAR1 is required to prevent T cell exhaustion and virus persistence in LCMV-Arm infection, and i...
Source: Viruses - March 1, 2024 Category: Virology Authors: Alanna G. Spiteri Tamara Suprunenko Erin Cutts Andrew Suen Thomas M. Ashhurst Barney Viengkhou Nicholas J. C. King Markus J. Hofer Tags: Article Source Type: research
