Germ fate determinants protect germ precursor cell division by reducing septin and anillin levels at the cell division plane
Mol Biol Cell. 2024 May 2:mbcE24020096T. doi: 10.1091/mbc.E24-02-0096-T. Online ahead of print.ABSTRACTAnimal cell cytokinesis, or the physical division of one cell into two, is thought to be driven by constriction of an actomyosin contractile ring at the division plane. The mechanisms underlying cell type-specific differences in cytokinesis remain unknown. Germ cells are totipotent cells that pass genetic information to the next generation. Previously, using formincyk-1(ts) mutant C. elegans embryos, we found that the P2 germ precursor cell is protected from cytokinesis failure and can divide with greatly reduced F-actin ...
Source: Mol Biol Cell - May 2, 2024 Category: Molecular Biology Authors: Caroline Q Connors Michael S Mauro J Tristian Wiles Andrew D Countryman Sophia L Martin Benjamin Lacroix Mimi Shirasu-Hiza Julien Dumont Karen E Kasza Timothy R Davies Julie C Canman Source Type: research
Ultraviolet Light-Emitting Diode Radiation Kills Leukemia Cells Without Affecting Hematopoiesis of Hematopoietic Stem Cells in Mice
CONCLUSIONS: Our results suggest that ultraviolet LED radiation can suppress the proliferative and tumorigenic abilities of leukemia cells without reducing the hematopoietic reconstitution capacity of hematopoietic stem cells, serving as a promising approach to kill leukemia cells in autograft before autologous hematopoietic stem cell transplant.PMID:38695592 | DOI:10.6002/ect.2023.0339 (Source: Experimental and Clinical Transplantation : official journal of the Middle East Society for Organ Transplantation)
Source: Experimental and Clinical Transplantation : official journal of the Middle East Society for Organ Transplantation - May 2, 2024 Category: Transplant Surgery Authors: Dong Xie Lirong Sun Xingqing Guo Jian Jiang Yan Sun Jiandong Sun Source Type: research
Outcomes and genetic dynamics of acute myeloid leukemia at first relapse
Haematologica. 2024 May 2. doi: 10.3324/haematol.2024.285057. Online ahead of print.ABSTRACTPatients with relapsed acute myeloid leukemia (rAML) experience dismal outcomes. We performed a comprehensive analysis of patients with rAML to determine the genetic dynamics and survival predictive factors. We analyzed 875 patients with newly diagnosed AML who received intensive treatment (IT) or low-intensity treatment (LIT). Of these patients, 197 experienced subsequent rAML. Data was available for 164 patients, with a median time from CR/CRi to relapse of 6.5 months. Thirty-five of the 164 patients (21%) experienced relapse afte...
Source: Haematologica - May 2, 2024 Category: Hematology Authors: Alex Bataller Hagop Kantarjian Alexandre Bazinet Tapan Kadia Naval Daver Courtney D DiNardo Gautam Borthakur Sanam Loghavi Keyur Patel Guilin Tang Koji Sasaki Nicholas J Short Musa Yilmaz Ghayas C Issa Yesid Alvarado Guillermo Montalban-Bravo Abhishek Mai Source Type: research
Daratumumab and antineoplastic therapy versus antineoplastic therapy only for adults with newly diagnosed multiple myeloma ineligible for transplant
CONCLUSIONS: Overall analysis of four studies showed a potential benefit for daratumumab in terms of overall survival and progression-free survival and a slight potential benefit in quality of life. Participants treated with daratumumab probably experience increased serious adverse events. There were likely no differences between groups in adverse events (CTCAE grade ≥ 3); however, there are probably more infections (CTCAE grade ≥ 3) in participants treated with daratumumab. We identified six ongoing studies which might strengthen the certainty of evidence in a future update of this review.PMID:38695605 | DOI:10.1002/1...
Source: Cochrane Database of Systematic Reviews - May 2, 2024 Category: General Medicine Authors: Peter Langer Lukas John Ina Monsef Christof Scheid Vanessa Piechotta Nicole Skoetz Source Type: research
From islet transplantation to beta-cell regeneration: an update on beta-cell-based therapeutic approaches in type 1 diabetes
Expert Rev Endocrinol Metab. 2024 May 1:1-11. doi: 10.1080/17446651.2024.2347263. Online ahead of print.ABSTRACTINTRODUCTION: Type 1 diabetes (T1D) mellitus is an autoimmune disease in which immune cells, predominantly effector T cells, destroy insulin-secreting beta-cells. Beta-cell destruction led to various consequences ranging from retinopathy and nephropathy to neuropathy. Different strategies have been developed to achieve normoglycemia, including exogenous glucose compensation, whole pancreas transplantation, islet transplantation, and beta-cell replacement.AREAS COVERED: The last two decades of experience have show...
Source: Expert Review of Endocrinology and Metabolism - May 2, 2024 Category: Endocrinology Authors: Asef Azad Hasan Ali Altunbas Ayse Esra Manguoglu Source Type: research
Recent advances on anti-HIV chimeric antigen receptor-T-cell treatment to provide sustained HIV remission
Curr Opin HIV AIDS. 2024 May 2. doi: 10.1097/COH.0000000000000858. Online ahead of print.ABSTRACTPURPOSE OF REVIEW: Successful sustained remission of HIV infection has been achieved after CCR5Δ32/Δ32 allogeneic hematopoietic stem cell transplantation for treatment of leukemia in a small cohort of people living with HIV (PLWH). This breakthrough demonstrated that the goal of curing HIV was achievable. However, the high morbidity and mortality associated with bone marrow transplantation limits the routine application of this approach and provides a strong rationale for pursuing alternative strategies for sustained long-ter...
Source: Cancer Control - May 2, 2024 Category: Cancer & Oncology Authors: Hang Su April Mueller Harris Goldstein Source Type: research
Use of iPSC-Derived Smooth Muscle Cells to Model Physiology and Pathology
Arterioscler Thromb Vasc Biol. 2024 May 2. doi: 10.1161/ATVBAHA.123.319703. Online ahead of print.ABSTRACTThe implementation of human-induced pluripotent stem cell models has introduced an additional tool for identifying molecular mechanisms of disease that complement animal models. Patient-derived or CRISPR/Cas9-edited induced pluripotent stem cells differentiated into smooth muscle cells (SMCs) have been leveraged to discover novel mechanisms, screen potential therapeutic strategies, and model in vivo development. The field has evolved over almost 15 years of research using human-induced pluripotent stem cell-SMCs and ha...
Source: Arteriosclerosis, Thrombosis and Vascular Biology - May 2, 2024 Category: Cardiology Authors: Callie S Kwartler Jose Emiliano Esparza Pinelo Source Type: research
GSE264206 A culture method with berbamine, a plant alkaloid, enhances CAR-T cell efficacy through modulating cellular metabolism
Contributors : Shin-ichiro Takayanagi ; Sayaka Chuganji ; Ken Fukumoto ; Shin KanekoSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensMemory T cells demonstrate superior in vivo persistence and antitumor efficacy. However, methods for manufacturing less differentiated T cells are not yet well-established. Here, we show that producing chimeric antigen receptor (CAR)-T cells using berbamine (BBM), a natural compound found in the Chinese herbal medicine Berberis amurensis, enhances the antitumor efficacy of CAR-T cells. BBM is identified through cell-based screening of chemical compoun...
Source: GEO: Gene Expression Omnibus - May 2, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
The effect of diabetes mellitus on differentiation of mesenchymal stem cells into insulin-producing cells
Diabetes mellitus (DM) is a global epidemic with increasing incidences. DM is a metabolic disease associated with chronic hyperglycemia. Aside from conventional treatments, there is no clinically approved cure... (Source: Biological Research)
Source: Biological Research - May 2, 2024 Category: Biomedical Science Authors: Omar I. Badr, Mohamed M. Kamal, Shohda A. El-Maraghy and Heba R. Ghaiad Tags: Research article Source Type: research
Emerging DNA Methylome Targets in FLT3-ITD-Positive Acute Myeloid Leukemia: Combination Therapy with Clinically Approved FLT3 Inhibitors
Opinion statementThe internal tandem duplication (ITD) mutation of the FMS-like receptor tyrosine kinase 3 (FLT3-ITD) is the most common mutation observed in approximately 30% of acute myeloid leukemia (AML) patients. It represents poor prognosis due to continuous activation of downstream growth-promoting signaling pathways such as STAT5 and PI3K/AKT. Hence, FLT3 is considered an attractive druggable target; selective small FLT3 inhibitors (FLT3Is), such as midostaurin and quizartinib, have been clinically approved. However, patients possess generally poor remission rates and acquired resistance when FLT3I used alone. Vari...
Source: Current Treatment Options in Oncology - May 2, 2024 Category: Cancer & Oncology Source Type: research
Continuously improving outcome over time after second allogeneic stem cell transplantation in relapsed acute myeloid leukemia: an EBMT registry analysis of 1540 patients
Blood Cancer Journal, Published online: 02 May 2024; doi:10.1038/s41408-024-01060-4Continuously improving outcome over time after second allogeneic stem cell transplantation in relapsed acute myeloid leukemia: an EBMT registry analysis of 1540 patients (Source: Blood Cancer Journal)
Source: Blood Cancer Journal - May 2, 2024 Category: Hematology Authors: Ann-Kristin Schm älter Maud Ngoya Jacques-Emmanuel Galimard Ali Bazarbachi J ürgen Finke Nicolaus Kr öger Martin Bornh äuser Matthias Stelljes Friedrich St ölzel Johanna Tischer Thomas Schroeder Peter Dreger Igor-Wolfgang Blau Bipin Savani Sebastian Source Type: research
Drug-regulated CD33-targeted CAR T cells control AML using clinically optimized rapamycin dosing
Chimeric antigen receptor (CAR) designs that incorporate pharmacologic control are desirable; however, designs suitable for clinical translation are needed. We designed a fully human, rapamycin-regulated drug product for targeting CD33+ tumors called dimerizaing agent–regulated immunoreceptor complex (DARIC33). T cell products demonstrated target-specific and rapamycin-dependent cytokine release, transcriptional responses, cytotoxicity, and in vivo antileukemic activity in the presence of as little as 1 nM rapamycin. Rapamycin withdrawal paused DARIC33-stimulated T cell effector functions, which were restored following r...
Source: Journal of Clinical Investigation - May 1, 2024 Category: Biomedical Science Authors: Jacob Appelbaum, April E. Price, Kaori Oda, Joy Zhang, Wai-Hang Leung, Giacomo Tampella, Dong Xia, Pauline P.L. So, Sarah K. Hilton, Claudya Evandy, Semanti Sarkar, Unja Martin, Anne-Rachel Krostag, Marissa Leonardi, Daniel E. Zak, Rachael Logan, Paula Le Source Type: research
High CD34-positive cell dose in matched unrelated donor allogeneic hematopoietic stem cell transplant is not associated with graft-versus-host disease or mortality
DISCUSSION: We found no difference in survival, relapse, or GVHD incidence or severity in patients receiving CD34+ doses above prior cutoffs reported in the literature. These data do not support the routine use of graft CD34+ dose reduction.PMID:38693089 | DOI:10.1111/trf.17864 (Source: Transfusion)
Source: Transfusion - May 1, 2024 Category: Hematology Authors: Zachary M Avigan Ajoy L Dias Laura E Dodge Jon E Arnason Robin M Joyce Jessica Liegel Jacalyn Rosenblatt Matthew J Weinstock David E Avigan Richard L Haspel Source Type: research
Prevention of post-operative adhesions: Model development and pilot outcomes of human placental stem cell-based interventions
This study presents a novel rat model for abdominal adhesions and reports pilot results of human placental stem cell (hPSC)-based therapies.METHODS: Forty-four (n = 44) male Sprague-Dawley rats (250-350 g) were used in the experiment. Of these, thirty-eight (n = 38) were included in a preliminary data set to determine a minimum treatment effect. Adhesions were created in a reproducible model to the abdominal wall and between organs. Experimental groups included the control group (Model No Treatment, MNT), Plasmalyte A (Media Alone, MA, 10 mL), hPSC (5 × 106 cells/10 mL Plasmalyte A), hPSC-CM (hPSC secretome, conditioned m...
Source: Transfusion - May 1, 2024 Category: Hematology Authors: Samuel P Carmichael Prafulla K Chandra John W Vaughan David M Kline Edward H Ip John B Holcomb Anthony Atala Source Type: research
The Effect of Glycyrrhizin on the Viability and Proliferation of Dental Pulp Stem Cells Compared to Intracanal Medicaments
CONCLUSION: Based upon the potential of GA on DPSCs proliferation compared with Ca(OH)2 and TAP, It is conceivable to acknowledge that GA could be used as an intracanal medicaments for revascularization process of necrotic immature teeth.CLINICAL SIGNIFICANCE: This study emphasizes the significance of assessing alternative root canal medicaments and their impact on the proliferation and viability of DPSCs. The results regarding GA, specifically its impact on the viability and growth of DPSCs, provide essential understanding for its potential application as an intracanal medicine. This study adds to the continuous endeavors...
Source: J Contemp Dent Pract - May 1, 2024 Category: Dentistry Authors: Mohammad A Alrashidi Manal F Badawi Mohamed G Elbeltagy Amany E Badr Source Type: research
