GSE244362 Genome-Wide CRISPR Screen Identifies an NF2-adheren junction mechanistic dependency for cardiac lineage
Contributors : Chang Jie Mick Lee ; Roger S FooSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCardiac differentiation involves a stepwise clearance of repressors and fate-restricting regulators through the modulation of BMP/Wnt-signaling pathways. However, the mechanisms and how regulatory roadblocks are removed with specific developmental signaling pathways remain unclear. Here, we performed a genome-wide CRISPR screen to uncover essential regulators of cardiomyocyte specification in human embryonic stem cells (hESCs) and identify NF2, a Moesin-Ezrin-Radixin Like (MERLIN) Tumor Su...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE246792 Bradykinin promotes immune responses in differentiated embryonic neurospheres carrying APP swe and PS1 dE9 mutations
Contributors : Guilherme Juvenal ; Carine Meinerz ; Ana C Ayupe ; Henrique C Campos ; Eduardo M Reis ; Beatriz M Longo ; Micheli M Pillat ; Henning UlrichSeries Type : Expression profiling by arrayOrganism : Mus musculusNeural stem cells (NSCs) can be cultivated from developing brains, reproducing many of the processes that occur during neural development. They can be isolated from a variety of animal models, such as transgenic mice carrying mutations in amyloid precursor protein (APP) and presenilin 1 and 2 (PSEN 1 and 2), characteristic of familial Alzheimer ’s Disease (AD). Modulating the development of these cells...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research
GSE248027 ASXL1/2 links MLL4 and BAP1 on enhancers
Contributors : Xie Guojia ; Lee Ji-Eun ; Ge KaiSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusHistone H3K4 monomethyltransferases MLL3 and MLL4 contain a set of uncharacterized PHD fingers. By structural and biochemical assays, we found a novel function of the PHD2 and PHD3 (PHD2/3) fingers of MLL3 and MLL4, revealing their direct binding to the conserved MBH (MLL binding helix) region of ASXL1/2, components of the Polycomb repressive PR-DUB complex. In mouse embryonic stem cells, we observed that BAP1, the catalytic subunit of the PR-DUB complex, physically interact...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Mus musculus Source Type: research
GSE230441 Isoform-specific translational control is evolutionarily conserved in primates
Contributors : Jolene Draper ; Julia Philipp ; Zach Neeb ; Richard Thomas ; Solomon Katzman ; Sofie Salama ; David Haussler ; Jeremy SanfordSeries Type : OtherOrganism : Homo sapiens ; Pan troglodytes ; Pongo abeliiAlternative splicing (AS) influences the expression of human genes in diverse ways. We previously used subcellular fraction-sequencing (Frac-Seq) to reveal an unexpected connection between alternative splicing and isoform-specific mRNA translation. Here we apply comparative transcriptomics to explore alternative splicing coupled translational control (AS-TC) across 13 million years of primate evolution. ...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Other Homo sapiens Pan troglodytes Pongo abelii Source Type: research
GSE230451 Single-nucleus transcriptomic sequencing and cross integration comparison of multiple species revealed new cell composition and specific gene marker in hippocampal aging
Contributors : Shi Lan-Lan ; Rui-Ze Niu ; Jia LiuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusBrain aging is a major risk factor for many brain diseases, which seriously affects the quality of life and even life span, and poses an increasingly serious threat to human health. Research shows that anti-aging and an extra year of life expectancy could bring $38 trillion in economic gains. Therefore, it is urgent to study the mechanism of brain aging, find effective ways to improve aging and improve the quality of life of the aging population. In the early stage, we established the c...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE202320 Follistatin-like 1 guarantees the immunomodulatory properties of mesenchymal stem cells during liver fibrotic therapy
Contributors : Xiaohong Zheng ; Ying HanSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensMesenchymal stem cell (MSC) therapy has been shown to be a promising option for liver fibrosis treatment. However, critical factors affecting the efficacy of MSC therapy for liver fibrosis remain unknown. Follistatin-like 1 (FSTL1), a TGF- -induced matricellular protein, is documented as an intrinsic regulator of proliferation and differentiation in MSCs. In the present study, we characterized the potential role of FSTL1 in MSC-based anti-fibrotic therapy and further elucidated the mechanisms ...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE130288 The effect of acidosis on adipose-derived stem cell
In this study, different pH levels of medium were performed to treat adipose-derived stem cells (ADSCs) with 24/48h time- exposure, to explore the effect of extracellular acidosis on ADSCs. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Effects of maternal exercise on infant mesenchymal stem cell mitochondrial function, insulin action, and body composition in infancy
AbstractMaternal exercise (ME) has been established as a useful non-pharmacological intervention to improve infant metabolic health; however, mechanistic insight behind these adaptations remains mostly confined to animal models. Infant mesenchymal stem cells (MSCs) give rise to infant tissues (e.g., skeletal muscle), and remain involved in mature tissue maintenance. Importantly, these cells maintain metabolic characteristics of an offspring donor and provide a model for the investigation of mechanisms behind infant metabolic health improvements. We used undifferentiated MSC to investigate if ME affects infant MSC mitochond...
Source: Physiological Reports - April 30, 2024 Category: Physiology Authors: Filip Jevtovic,
Donghai Zheng,
Alex Claiborne,
Ericka M. Biagioni,
Breanna L. Wisseman,
Polina M. Krassovskaia,
David N. Collier,
Christy Isler,
James E. DeVente,
P. Darrell Neufer,
Joseph A. Houmard,
Linda E. May Tags: ORIGINAL ARTICLE Source Type: research
Letermovir primary cytomegalovirus prophylaxis in allogeneic hematopoietic cell transplant recipients: could infection and disease no longer be a significant problem?
Conclusions: LET primary prophylaxis proved effective in preventing cs-CMVi and CMVd and reducing hospitalizations in allo-HCT adults. Blips can occur during prophylaxis and do not require LET discontinuation.Keywords: letermovir; cytomegalovirus; hematopoietic cell transplantation. (Source: Mediterranean Journal of Hematology and Infectious Diseases)
Source: Mediterranean Journal of Hematology and Infectious Diseases - April 30, 2024 Category: Hematology Authors: Fabian Herrera Source Type: research
Survival After Hematopoietic Stem Cell Transplantation in Severe Combined Immunodeficiency (SCID): A Worldwide Review of the Prognostic Variables
This study aims to perform an extensive review of the literature that evaluates various factors that affect the survival rates of patients with severe combined immunodeficiency (SCID) after hematopoietic stem cell transplantation (HSCT) in developed and developing countries. An extensive search of the literature was made in four different databases (PubMed, Embase, Scopus, and Web of Science). The search was carried out in December 2022 and updated in July 2023, and the terms such as “hematopoietic stem cell transplantation,” “bone marrow transplant,” “mortality,” “opportunistic infections,” and “survival...
Source: Clinical Reviews in Allergy and Immunology - April 30, 2024 Category: Allergy & Immunology Source Type: research
Feasibility of intranasal human milk as stem cell therapy in preterm infants with intraventricular hemorrhage
Journal of Perinatology, Published online: 30 April 2024; doi:10.1038/s41372-024-01982-8Feasibility of intranasal human milk as stem cell therapy in preterm infants with intraventricular hemorrhage (Source: Journal of Perinatology)
Source: Journal of Perinatology - April 30, 2024 Category: Perinatology & Neonatology Authors: Rebecca Hoban Alessia Gallipoli Marisa Signorile Poonam Mander Andree Gauthier-Fisher Clifford Librach Diane Wilson Sharon Unger Source Type: research
High-fat diet enhances cell proliferation and compromises intestinal permeability in a translational canine intestinal organoid model
Emerging evidence underscores the responsiveness of the mammalian intestine to dietary cues, notably through the involvement of LGR5 + intestinal stem cells in orchestrating responses to diet-driven signals. H... (Source: BMC Cell Biology)
Source: BMC Cell Biology - April 30, 2024 Category: Cytology Authors: Itsuma Nagao and Yoko M. Ambrosini Tags: Research Source Type: research
Molecules, Vol. 29, Pages 2080: Application of Cinnamomum burmannii Essential Oil in Promoting Wound Healing
This study analyzed 32 main components and their relative contents of essential oil using GC-MS. Then, network pharmacology was used to predict the possible targets of this essential oil in wound healing. We first proved this essential oil’s effects in vitro and in vivo. Cinnamomum burmannii essential oil could not only promote the proliferation and migration of skin stromal cells, but also promote M2-type polarization of macrophages while inhibiting the expression of pro-inflammatory cytokines. This study explored the possible mechanism by which Cinnamomum burmannii essential oil promotes wound healing, prov...
Source: Molecules - April 30, 2024 Category: Chemistry Authors: Xiangsheng Zhang Xueyi Lin Jiayuan Cao Guofeng Xie Xinrui Yang Bingnan Liu Xin Xu Fang Cheng Hongbo Chen Yuxin Pang Tags: Article Source Type: research
Low Initial Cell Density Promotes the Differentiation and Maturation of Human Pluripotent Stem Cells into Erythrocytes
Stem Cells and Development, Ahead of Print. (Source: Stem Cells and Development)
Source: Stem Cells and Development - April 30, 2024 Category: Stem Cells Authors: Liqing Liang Lei Xu Qian Dong Jing Zhang Mingyi Qu Xin Yuan Quan Zeng Huilin Li Bowen Zhang Chao Wang Tao Fan Lijuan He Wen Yue Xiaoyan Xie Xuetao Pei Source Type: research
Cancers, Vol. 16, Pages 1754: Antiretroviral Drug Repositioning for Glioblastoma
rine DeMarino
John D. Heiss
Avindra Nath
Ashish H. Shah
Outcomes for glioblastoma (GBM) remain poor despite standard-of-care treatments including surgical resection, radiation, and chemotherapy. Intratumoral heterogeneity contributes to treatment resistance and poor prognosis, thus demanding novel therapeutic approaches. Drug repositioning studies on antiretroviral therapy (ART) have shown promising potent antineoplastic effects in multiple cancers; however, its efficacy in GBM remains unclear. To better understand the pleiotropic anticancer effects of ART on GBM, we conducted a comprehensive drug repurposing ana...
Source: Cancers - April 30, 2024 Category: Cancer & Oncology Authors: Sarah R. Rivas Mynor J. Mendez Valdez Jay S. Chandar Jelisah F. Desgraves Victor M. Lu Leo Ampie Eric B. Singh Deepa Seetharam Christian K. Ramsoomair Anna Hudson Shreya M. Ingle Vaidya Govindarajan Tara T. Doucet-O ’Hare Catherine DeMarino John D. Heis Tags: Article Source Type: research
