Myeloablation Followed by Hematopoietic Stem Cell Transplantation and Long ‐Term Normalization of Systemic Sclerosis Molecular Signatures
ConclusionParalleling the observed clinical benefit, HSCT leads to durable long-term normalization of the molecular signature in SSc, with completion of immune resetting to 54 months after HSCT. (Source: Arthritis and Rheumatology)
Source: Arthritis and Rheumatology - April 30, 2024 Category: Rheumatology Authors: Nancy Wareing,
Xuan Wang,
Lynette Keyes ‐Elstein,
Ellen A. Goldmuntz,
Marka A. Lyons,
Peter McSweeney,
Daniel E. Furst,
Richard A. Nash,
Leslie J. Crofford,
Beverly Welch,
Ashley Pinckney,
Maureen D. Mayes,
Keith M. Sullivan,
Shervin Assass Tags: Brief Report Source Type: research
GSE265827 Embryonic myeloid cells orchestrate niche cell homeostasis critical for the establishment of the definitive HSC pool
Contributors : G ülce Perçin ; Konstantin Riege ; Stephan Culemann ; Mathias Lesche ; Susanne Reinhardt ; Steve Hoffmann ; Claudia WaskowSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe lifelong replenishment of blood cells relies on the function of definitive HSCs that migrate to the bone marrow during development. This process is tightly controlled by the bone marrow microenvironment. Embryonic macrophages emerge before the onset of definitive hematopoiesis, seed into discrete tissues and contribute to specialized resident macrophages throughout life. However, the functi...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE263174 CRISPR Screening Uncovers a Long-Range Enhancer for ONECUT1 in Pancreatic Differentiation and Links a Diabetes Risk Variant
Contributors : Danwei Huangfu ; Wilfred W Wong ; Samuel J KaplanSeries Type : OtherOrganism : Homo sapiensFunctional enhancer annotation is a valuable first step for understanding tissue-specific transcriptional regulation and prioritizing disease-associated non-coding variants for investigation. However, unbiased enhancer discovery in physiologically relevant contexts remains a major challenge. To discover regulatory elements pertinent to diabetes, we conducted a CRISPR interference (CRISPRi) screen in the human pluripotent stem cell (hPSC) pancreatic differentiation system. Among the enhancers uncovered, we focused on a...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Other Homo sapiens Source Type: research
GSE262787 The synergistic effect of pulsed-focused ultrasound and umbilical cord mesenchymal stem cells in facilitating recovery from acute kidney injury through mitochondrial regulation and heat shock proteins
In conclusion, our results show that the therapeutic effect of UC-MSCs for treating AKI c an be augmented by priming the kidney with repeated LpFUS before UC-MSC therapy. This innovative approach holds promise for advancing the treatment of AKI. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE255545 Calcineurin inhibition rescues alloantigen-specific central memory T cell subsets and promotes chronic GVHD
Contributors : Ping Zhang ; Olivia G Waltner ; Shruti S Bhise ; Scott N Furlan ; Simone A Minnie ; Geoffrey R HillSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusCalcineurin inhibitors (CNIs) constitute the backbone of modern acute graft-versus-host disease (aGVHD) prophylaxis regimens but have limited efficacy in the prevention and treatment of chronic GVHD (cGVHD). We investigated the effect of CNIs on immune tolerance after stem cell transplantation with discovery-based single cell gene expression and T-cell receptor (TCR) assays of clonal immunity in tandem with traditional prote...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE244362 Genome-Wide CRISPR Screen Identifies an NF2-adheren junction mechanistic dependency for cardiac lineage
Contributors : Chang Jie Mick Lee ; Roger S FooSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCardiac differentiation involves a stepwise clearance of repressors and fate-restricting regulators through the modulation of BMP/Wnt-signaling pathways. However, the mechanisms and how regulatory roadblocks are removed with specific developmental signaling pathways remain unclear. Here, we performed a genome-wide CRISPR screen to uncover essential regulators of cardiomyocyte specification in human embryonic stem cells (hESCs) and identify NF2, a Moesin-Ezrin-Radixin Like (MERLIN) Tumor Su...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE246792 Bradykinin promotes immune responses in differentiated embryonic neurospheres carrying APP swe and PS1 dE9 mutations
Contributors : Guilherme Juvenal ; Carine Meinerz ; Ana C Ayupe ; Henrique C Campos ; Eduardo M Reis ; Beatriz M Longo ; Micheli M Pillat ; Henning UlrichSeries Type : Expression profiling by arrayOrganism : Mus musculusNeural stem cells (NSCs) can be cultivated from developing brains, reproducing many of the processes that occur during neural development. They can be isolated from a variety of animal models, such as transgenic mice carrying mutations in amyloid precursor protein (APP) and presenilin 1 and 2 (PSEN 1 and 2), characteristic of familial Alzheimer ’s Disease (AD). Modulating the development of these cells...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research
GSE248027 ASXL1/2 links MLL4 and BAP1 on enhancers
Contributors : Xie Guojia ; Lee Ji-Eun ; Ge KaiSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusHistone H3K4 monomethyltransferases MLL3 and MLL4 contain a set of uncharacterized PHD fingers. By structural and biochemical assays, we found a novel function of the PHD2 and PHD3 (PHD2/3) fingers of MLL3 and MLL4, revealing their direct binding to the conserved MBH (MLL binding helix) region of ASXL1/2, components of the Polycomb repressive PR-DUB complex. In mouse embryonic stem cells, we observed that BAP1, the catalytic subunit of the PR-DUB complex, physically interact...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Mus musculus Source Type: research
GSE230441 Isoform-specific translational control is evolutionarily conserved in primates
Contributors : Jolene Draper ; Julia Philipp ; Zach Neeb ; Richard Thomas ; Solomon Katzman ; Sofie Salama ; David Haussler ; Jeremy SanfordSeries Type : OtherOrganism : Homo sapiens ; Pan troglodytes ; Pongo abeliiAlternative splicing (AS) influences the expression of human genes in diverse ways. We previously used subcellular fraction-sequencing (Frac-Seq) to reveal an unexpected connection between alternative splicing and isoform-specific mRNA translation. Here we apply comparative transcriptomics to explore alternative splicing coupled translational control (AS-TC) across 13 million years of primate evolution. ...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Other Homo sapiens Pan troglodytes Pongo abelii Source Type: research
GSE230451 Single-nucleus transcriptomic sequencing and cross integration comparison of multiple species revealed new cell composition and specific gene marker in hippocampal aging
Contributors : Shi Lan-Lan ; Rui-Ze Niu ; Jia LiuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusBrain aging is a major risk factor for many brain diseases, which seriously affects the quality of life and even life span, and poses an increasingly serious threat to human health. Research shows that anti-aging and an extra year of life expectancy could bring $38 trillion in economic gains. Therefore, it is urgent to study the mechanism of brain aging, find effective ways to improve aging and improve the quality of life of the aging population. In the early stage, we established the c...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
GSE202320 Follistatin-like 1 guarantees the immunomodulatory properties of mesenchymal stem cells during liver fibrotic therapy
Contributors : Xiaohong Zheng ; Ying HanSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensMesenchymal stem cell (MSC) therapy has been shown to be a promising option for liver fibrosis treatment. However, critical factors affecting the efficacy of MSC therapy for liver fibrosis remain unknown. Follistatin-like 1 (FSTL1), a TGF- -induced matricellular protein, is documented as an intrinsic regulator of proliferation and differentiation in MSCs. In the present study, we characterized the potential role of FSTL1 in MSC-based anti-fibrotic therapy and further elucidated the mechanisms ...
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE130288 The effect of acidosis on adipose-derived stem cell
In this study, different pH levels of medium were performed to treat adipose-derived stem cells (ADSCs) with 24/48h time- exposure, to explore the effect of extracellular acidosis on ADSCs. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 30, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Effects of maternal exercise on infant mesenchymal stem cell mitochondrial function, insulin action, and body composition in infancy
AbstractMaternal exercise (ME) has been established as a useful non-pharmacological intervention to improve infant metabolic health; however, mechanistic insight behind these adaptations remains mostly confined to animal models. Infant mesenchymal stem cells (MSCs) give rise to infant tissues (e.g., skeletal muscle), and remain involved in mature tissue maintenance. Importantly, these cells maintain metabolic characteristics of an offspring donor and provide a model for the investigation of mechanisms behind infant metabolic health improvements. We used undifferentiated MSC to investigate if ME affects infant MSC mitochond...
Source: Physiological Reports - April 30, 2024 Category: Physiology Authors: Filip Jevtovic,
Donghai Zheng,
Alex Claiborne,
Ericka M. Biagioni,
Breanna L. Wisseman,
Polina M. Krassovskaia,
David N. Collier,
Christy Isler,
James E. DeVente,
P. Darrell Neufer,
Joseph A. Houmard,
Linda E. May Tags: ORIGINAL ARTICLE Source Type: research
Letermovir primary cytomegalovirus prophylaxis in allogeneic hematopoietic cell transplant recipients: could infection and disease no longer be a significant problem?
Conclusions: LET primary prophylaxis proved effective in preventing cs-CMVi and CMVd and reducing hospitalizations in allo-HCT adults. Blips can occur during prophylaxis and do not require LET discontinuation.Keywords: letermovir; cytomegalovirus; hematopoietic cell transplantation. (Source: Mediterranean Journal of Hematology and Infectious Diseases)
Source: Mediterranean Journal of Hematology and Infectious Diseases - April 30, 2024 Category: Hematology Authors: Fabian Herrera Source Type: research
Survival After Hematopoietic Stem Cell Transplantation in Severe Combined Immunodeficiency (SCID): A Worldwide Review of the Prognostic Variables
This study aims to perform an extensive review of the literature that evaluates various factors that affect the survival rates of patients with severe combined immunodeficiency (SCID) after hematopoietic stem cell transplantation (HSCT) in developed and developing countries. An extensive search of the literature was made in four different databases (PubMed, Embase, Scopus, and Web of Science). The search was carried out in December 2022 and updated in July 2023, and the terms such as “hematopoietic stem cell transplantation,” “bone marrow transplant,” “mortality,” “opportunistic infections,” and “survival...
Source: Clinical Reviews in Allergy and Immunology - April 30, 2024 Category: Allergy & Immunology Source Type: research
