Thrombopoietin Agonism to Promote Platelet Engraftment Following Hematopoietic Stem Cell Transplantation: Promising, but Not Ready for Primetime
In this issue of TCT, Fu and colleagues report the results of a randomized clinical trial conducted in China comparing thrombopoietic support using recombinant human thrombopoietin (rhTPO) with observation to promote platelet engraftment in patients receiving haploidentical hematopoietic stem cell transplantation (HSCT) with post-transplantation cyclophosphamide for the treatment of severe aplastic anemia (SAA).1 The trial enrolled 55 patients, randomized 1:1 to rhTPO or observation. The authors report successful hematopoietic reconstitution in all patients, but significantly faster platelet engraftment in patients randomi...
Source: Biology of Blood and Marrow Transplantation - April 26, 2024 Category: Hematology Authors: Hanny Al-Samkari Tags: The Bottom Line Source Type: research
Cellular strategies to induce immune tolerance after liver transplantation: Clinical perspectives
World J Gastroenterol. 2024 Apr 7;30(13):1791-1800. doi: 10.3748/wjg.v30.i13.1791.ABSTRACTLiver transplantation (LT) has become the most efficient treatment for pediatric and adult end-stage liver disease and the survival time after transplantation is becoming longer due to the development of surgical techniques and perioperative management. However, long-term side-effects of immunosuppressants, like infection, metabolic disorders and malignant tumor are gaining more attention. Immune tolerance is the status in which LT recipients no longer need to take any immunosuppressants, but the liver function and intrahepatic histol...
Source: World Journal of Gastroenterology - April 25, 2024 Category: Gastroenterology Authors: Ai-Wei Zhou Jing Jin Yuan Liu Source Type: research
Cellular strategies to induce immune tolerance after liver transplantation: Clinical perspectives
World J Gastroenterol. 2024 Apr 7;30(13):1791-1800. doi: 10.3748/wjg.v30.i13.1791.ABSTRACTLiver transplantation (LT) has become the most efficient treatment for pediatric and adult end-stage liver disease and the survival time after transplantation is becoming longer due to the development of surgical techniques and perioperative management. However, long-term side-effects of immunosuppressants, like infection, metabolic disorders and malignant tumor are gaining more attention. Immune tolerance is the status in which LT recipients no longer need to take any immunosuppressants, but the liver function and intrahepatic histol...
Source: World Journal of Gastroenterology : WJG - April 25, 2024 Category: Gastroenterology Authors: Ai-Wei Zhou Jing Jin Yuan Liu Source Type: research
A Deeper Depth of Response After Salvage Therapy Improves Outcomes of Autologous Stem Cell Transplantation in Relapsed Lymphoma and the Feasibility of Non-controlled Rate Freezing of Peripheral Blood Stem Cells
Conclusion High-dose therapy and autologous stem cell transplantation in low- to middle-income countries are limited to relatively younger patients, potentially curative conditions such as lymphoma, and predominantly after achieving a complete response to salvage therapy due to limited resources. Due to these factors, our study shows excellent response rates and survival outcomes compared to internationally published data. Engraftment was also excellent and comparable to published data despite the non-controlled rate freezing of peripheral blood stem cells.PMID:38659569 | PMC:PMC11039573 | DOI:10.7759/cureus.56851 (Source: Cancer Control)
Source: Cancer Control - April 25, 2024 Category: Cancer & Oncology Authors: Saif Ur Rab Mussadique Ali Uzma Rasool Mahar Bushra Ahsan Usman Ahmad Muhammad Tariq Mahmood Neelam Siddiqui Syed W Bokhari Source Type: research
BAG3 regulates cilia homeostasis of glioblastoma via its WW domain
Building on our previously identified and novel deciliating function of BAG3, we further decipher the molecular mechanisms of BAG3-regulated cilia homeostasis in glioblastoma (GBM). Based on identification of BAG3 interactomes and the contribution of different functional domains we now demonstrate that BAG3 regulates cilia homeostasis via its WW domain and modulation of YAP1 activity/downstream YAP1-dependent signaling. We also provide evidence for a complex interaction network of BAG3 with several pathways regulating cilia homeostasis at the level of ciliogenesis and cilium degradation. AbstractThe multidomain protein BAG...
Source: BioFactors - April 25, 2024 Category: Biochemistry Authors: Caterina Roth,
Lara Paulini,
Marina E. Hoffmann,
Thorsten Mosler,
Ivan Dikic,
Andreas Brunschweiger,
Hagen K örschgen,
Christian Behl,
Benedikt Linder,
Donat Kögel Tags: RESEARCH ARTICLE Source Type: research
Successful Stem Cell Transplantation from Varicella Infected Donor
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - April 25, 2024 Category: Hematology Source Type: research
In Memoriam: Prof. Makio Ogawa
Dr. Makio Ogawa, a pioneer and trailblazer in stem cell research, passed away on February 6, 2024, at his home in New Hampshire, just after celebrating his 84th birthday with a spirited online game of Go (a game much like checkers); his wife Mary-Jane found him peacefully at rest after he did not respond to a routine lunchtime call. (Source: Experimental Hematology)
Source: Experimental Hematology - April 25, 2024 Category: Hematology Authors: Keisuke Ito, Richard Stanley, Toshio Suda Tags: Obituary Source Type: research
Exosomes From Human Umbilical Cord Stem Cells Suppress Macrophage-to-myofibroblast Transition, Alleviating Renal Fibrosis
This study investigates their potential to lessen renal fibrosis by targeting macrophage-to-myofibroblast transformation (MMT), a key driver of fibrosis. We employed a mouse model of unilateral ureteral obstruction (UUO) and cultured cells exposed to transforming growth factor-β (TGF-β) to mimic MMT. HucMSC-Exos were administered to UUO mice, and their effects on kidney function and fibrosis were assessed. Additionally, RNA sequencing and cellul ar analysis were performed to elucidate the mechanisms by which HucMSC-Exos inhibit MMT. HucMSC-Exos treatment significantly reduced kidney damage and fibrosis in UUO mice. Th...
Source: Inflammation - April 25, 2024 Category: Allergy & Immunology Source Type: research
The genome of the colonial hydroid Hydractinia reveals that their stem cells use a toolkit of evolutionarily shared genes with all animals [RESOURCES]
Hydractinia is a colonial marine hydroid that shows remarkable biological properties, including the capacity to regenerate its entire body throughout its lifetime, a process made possible by its adult migratory stem cells, known as i-cells. Here, we provide an in-depth characterization of the genomic structure and gene content of two Hydractinia species, Hydractinia symbiolongicarpus and Hydractinia echinata, placing them in a comparative evolutionary framework with other cnidarian genomes. We also generated and annotated a single-cell transcriptomic atlas for adult male H. symbiolongicarpus and identified cell-type marker...
Source: Genome Research - April 25, 2024 Category: Genetics & Stem Cells Authors: Schnitzler, C. E., Chang, E. S., Waletich, J., Quiroga-Artigas, G., Wong, W. Y., Nguyen, A.-D., Barreira, S. N., Doonan, L. B., Gonzalez, P., Koren, S., Gahan, J. M., Sanders, S. M., Bradshaw, B., DuBuc, T. Q., Febrimarsa, , de Jong, D., Nawrocki, E. P., Tags: RESOURCES Source Type: research
Systematic mapping of TF-mediated cell fate changes by a pooled induction coupled with scRNA-seq and multi-omics approaches [METHODS]
Transcriptional regulation controls cellular functions through interactions between transcription factors (TFs) and their chromosomal targets. However, understanding the fate conversion potential of multiple TFs in an inducible manner remains limited. Here, we introduce iTF-seq as a method for identifying individual TFs that can alter cell fate toward specific lineages at a single-cell level. iTF-seq enables time course monitoring of transcriptome changes, and with biotinylated individual TFs, it provides a multi-omics approach to understanding the mechanisms behind TF-mediated cell fate changes. Our iTF-seq study in mouse...
Source: Genome Research - April 25, 2024 Category: Genetics & Stem Cells Authors: Lee, M., Guo, Q., Kim, M., Choi, J., Segura, A., Genceroglu, A., LeBlanc, L., Ramirez, N., Jang, Y. J., Jang, Y., Lee, B.-K., Marcotte, E. M., Kim, J. Tags: METHODS Source Type: research
Insights into treatment of complex Crohn's perianal fistulas
AbstractComplex perianal fistula is a common complication of Crohn ’s disease (CD) which leads to negative impact on patient’s quality of life. Successful management of the disease requires a multidisciplinary approach, including a gastroenterologist and a colorectal surgeon, applying combined surgical and medical therapy. One of frequently practiced surgical p rocedures is seton placement in the fistula tract, which is used to control perianal sepsis and drain the fistula, while preventing recurrent abscess formation.Darvadstrocel, a suspension of expanded, allogeneic, adipose-derived, mesenchymal stem cells, is safe ...
Source: BMC Proceedings - April 25, 2024 Category: Biomedical Science Source Type: research
Alternative pre-mRNA splicing in stem cell function and therapeutic potential: A critical review of current evidence
Int J Biol Macromol. 2024 Apr 22;268(Pt 1):131781. doi: 10.1016/j.ijbiomac.2024.131781. Online ahead of print.ABSTRACTAlternative splicing is a crucial regulator in stem cell biology, intricately influencing the functions of various biological macromolecules, particularly pre-mRNAs and the resultant protein isoforms. This regulatory mechanism is vital in determining stem cell pluripotency, differentiation, and proliferation. Alternative splicing's role in allowing single genes to produce multiple protein isoforms facilitates the proteomic diversity that is essential for stem cells' functional complexity. This review delves...
Source: International Journal of Biological Macromolecules - April 24, 2024 Category: Biochemistry Authors: Li Cui Yucheng Zheng Rongwei Xu Yunfan Lin Jiarong Zheng Pei Lin Bing Guo Shuyu Sun Xinyuan Zhao Source Type: research
Pediatric acute promyelocytic leukemia and Fanconi anemia: Case report and literature review
We report a 4 year-old boy from non-consanguineous parents diagnosed with standard risk APL. This child had café-au-lait spots and an extra thumb remnant. Genomic sequencing revealed two PV in FANCD1/BRCA2 confirming a diagnosis of FA-D1. Chromosomal breakage studies were compatible with FA. Each parent carried one variant and had no personal history of cancer. Morphological then molecular remissions were achieved with all-trans retinoic acid and Arsenic trioxide. This patient underwent haploidentical stem cell transplant. In addition to our patient, a literature search revealed four additional patients with APL/FA, with ...
Source: Clinical Genetics - April 24, 2024 Category: Genetics & Stem Cells Authors: Claire Freycon Edith Sepulchre Vincent-Philippe Lavall ée David Mitchell Margaret L MacMillan Catherine Vezina Catherine Goudie Source Type: research
Industry updates from the field of stem cell research and regenerative medicine in February 2024
Regen Med. 2024 Apr 24. doi: 10.2217/rme-2024-0046. Online ahead of print.ABSTRACTLatest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in February 2024.PMID:38655911 | DOI:10.2217/rme-2024-0046 (Source: Regenerative Medicine)
Source: Regenerative Medicine - April 24, 2024 Category: Genetics & Stem Cells Authors: Dusko Ilic Mirjana Liovic Source Type: research
GFRA4 improves the neurogenic potential of enteric neural crest stem cells via hedgehog pathway
CONCLUSION: GFRA4 promotes proliferation and differentiation but inhibits apoptosis of ENCSCs via the hedgehog pathway in HSCR.IMPACT: This study confirms that GFRA4 improves the proliferation and differentiation of ENCSCs via modulation of the hedgehog pathway. This study for the first time revealed the role and the mechanism of the action of GFRA4 in HSCR, which indicates that GFRA4 may play a role in the pathological development of HSCR. Our findings may lay the foundation for further investigation of the mechanisms underlying HSCR development and into targets of HSCR treatment.PMID:38658664 | DOI:10.1038/s41390-024-031...
Source: Pediatric Research - April 24, 2024 Category: Pediatrics Authors: Fangfang Zhang Mingyu Cui Lijuan Zhang Bangzhen Ma Feng Guo Gang Wang Source Type: research
