The interlacing anticancer effect of pharmacologic ascorbate, chloroquine, and resveratrol
Our present study first described that the combined Ph-Asc, Res, and CQ co-treatment exerted a synergistic cytotoxic effect on KRAS mutant PDAC cells. Both Ph-Asc and Res caused DSBs in the DNA, accompanied by cell cycle arrest and cell death. Ph-Asc-induced cytotoxicity shows strong ROS dependence, while Res-induced cytotoxicity seems to be independent of ROS generation; Res treatment induced significant elevation of caspase-3/7 activity while Ph-Asc-induced cell death proved to be caspase-independent. The fact that both Ph-Asc and Res are natural compounds without significant harmful effects on normal cells, and the thir...
Source: BioFactors - March 16, 2024 Category: Biochemistry Authors: Kinga Makk ‐Merczel, Dóra Varga, Péter Hajdinák, András Szarka Tags: RESEARCH ARTICLE Source Type: research

Resolvins protect against diabetes ‐induced colonic oxidative stress, barrier dysfunction, and associated diarrhea via the HO‐1 pathway
In conclusion, Oxidative stress in diabetes results in mucosal barrier dysfunction, contributing to the development of diabetic diarrhea. Resolvins prevent ROS-mediated mucosal injury and protect gut barrier function by intracellula r PI3K/Akt activation and subsequent HO-1 upregulation in intestinal epithelial cells. These actions result in normalizing stool frequency and stool water content in diabetic mice, suggesting that resolvins may be useful in the treatment of diabetic diarrhea. (Source: BioFactors)
Source: BioFactors - March 15, 2024 Category: Biochemistry Authors: Ting Yu, Die Chen, Hongyan Qi, Lin Lin, Yurong Tang Tags: RESEARCH ARTICLE Source Type: research

N ‐methyl‐d‐aspartate receptors: Structure, function, and role in organophosphorus compound poisoning
AlthoughN-methyl-d-aspartate receptor (NMDARs) are in normal physiological conditions important for controlling synaptic plasticity and mediating learning and memory functions, in this review, we elaborate the detrimental role NMDARs play in neurotoxicity of organophosphorus compounds and focus on the central role NMDAR inhibition plays in suppressing neurotoxicity and modulating the inflammatory response. AbstractAcute organophosphorus compound (OP) poisoning induces symptoms of the cholinergic crises with the occurrence of severe epileptic seizures. Seizures are induced by hyperstimulation of the cholinergic system, but ...
Source: BioFactors - February 29, 2024 Category: Biochemistry Authors: Dora Koli ć, Zrinka Kovarik Tags: REVIEW ARTICLE Source Type: research

Vitamin D metabolism is altered during aging alone or combined with obesity in male mice
We report for the first time the effect of aging process alone or in combination with obesity on vitamin D metabolism as a whole and leading to modulation of 25(OH)D plasma levels. Our data strongly suggest that the enzymes involved in vitamin D metabolism, notably in liver, kidneys, and adipose tissue, are transcriptionally modified under aging or combined effect of aging and obesity, contributing to the reduction of the plasma 25(OH)D. AbstractAging and obesity are associated with a decrease in plasma 25-hydroxyvitamin D (25(OH)D) levels. In the context of a growing aging population and the rising incidence of obesity, w...
Source: BioFactors - February 25, 2024 Category: Biochemistry Authors: Lorrine Bournot, Thomas Payet, Julie Marcotorchino, Manar Awada, Tha ïs Rouquet, Thomas Breniere, Charlène Couturier, Julien Astier, Charlotte Halimi, Emmanuelle Reboul, Flavie Sicard, Lourdes Mounien, Julien Roux, Bruno Bariohay, Jean Fra Tags: RESEARCH ARTICLE Source Type: research

Issue Information ‐ Cover
(Source: BioFactors)
Source: BioFactors - February 19, 2024 Category: Biochemistry Tags: ISSUE INFORMATION ‐ COVER Source Type: research

Issue Information
(Source: BioFactors)
Source: BioFactors - February 19, 2024 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research

Preface, February 2024 edition
(Source: BioFactors)
Source: BioFactors - February 19, 2024 Category: Biochemistry Authors: Peter D. Mace Tags: PREFACE Source Type: research

Issue Information ‐ Cover
(Source: BioFactors)
Source: BioFactors - February 19, 2024 Category: Biochemistry Tags: ISSUE INFORMATION ‐ COVER Source Type: research

Issue Information
(Source: BioFactors)
Source: BioFactors - February 19, 2024 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research

The functional roles of S ‐adenosyl‐methionine and S‐adenosyl‐homocysteine and their involvement in trisomy 21
Methionine-homocysteine cycle. The functional groups provided by S-adenosyl-methionine (SAM) and their usage are showed. SAH, S-adenosyl-homocysteine. AbstractThe one-carbon metabolism pathway is involved in critical human cellular functions such as cell proliferation, mitochondrial respiration, and epigenetic regulation. In the homocysteine-methionine cycle S-adenosyl-methionine (SAM) and S-adenosyl-homocysteine (SAH) are synthetized, and their levels are finely regulated to ensure proper functioning of key enzymes which control cellular growth and differentiation. Here we review the main biological mechanisms involving S...
Source: BioFactors - February 15, 2024 Category: Biochemistry Authors: Maria Caracausi, Giuseppe Ramacieri, Francesca Catapano, Michela Cicilloni, Bassam Lajin, Maria Chiara Pelleri, Allison Piovesan, Lorenza Vitale, Chiara Locatelli, Gian Luca Pirazzoli, Pierluigi Strippoli, Francesca Antonaros, Beatrice Vione Tags: REVIEW ARTICLE Source Type: research

Preface, February 2024 edition
(Source: BioFactors)
Source: BioFactors - February 14, 2024 Category: Biochemistry Authors: Peter D. Mace Tags: PREFACE Source Type: research

Ameliorating diabetes ‐induced testicular dysfunction by modulating PKC/Nrf2/Bcl‐2 signaling: Protective role of sulbutiamine
The objective of this study is to explore the potential protective effect of sulbutiamine against testicular hypofunction associated with streptozotocin (STZ)-induced DM in rats. Sulbutiamine was administered orally (60  mg/kg) to male Wistar rats for 8 weeks starting 72 h after a single injection of STZ (45 mg/kg, i.p.). Blood glucose level (BGL), serum testosterone level, sperm number, and motility were determined. Testicular tissue was examined histopathologically, and the Johnson score was evaluated. Le vels of malondialdehyde (MDA), protein kinase C (PKC), nuclear factor erythroid-derived 2-like 2 (Nrf2), and ...
Source: BioFactors - February 13, 2024 Category: Biochemistry Authors: Maha Abdelmonem, Shimaa O. Ali, Asmaa K. Al ‐Mokaddem, Heba R. Ghaiad Tags: RESEARCH ARTICLE Source Type: research