Factors determining the serum 25 ‐hydroxyvitamin D response to vitamin D supplementation: Data mining approach
We examined the relationship between 76 potential influencing factors on vitamin D response using RF. We found several features that were highly correlated to the serum vitamin D response to supplementation by RF including anthropometric factors (body mass index [BMI], free fat mass [FFM], fat percentage, waist-to-hip ratio [WHR]), liver function tests (serum gamma-glutamyl transferase [GGT], total bilirubin, total protein), hematological parameters (mean corpuscular volume [MCV], mean corpuscular hemoglobin concentration [MCHC], hematocrit), and measurement of insulin sensitivity (homeostatic model assessment of insulin r...
Source: BioFactors - July 18, 2021 Category: Biochemistry Authors: Zahra Amiri, Mina Nosrati, Payam Sharifan, Sara Saffar Soflaei, Susan Darroudi, Hamideh Ghazizadeh, Maryam Mohammadi Bajgiran, Fahimeh Moafian, Maryam Tayefi, Elahe Hasanzade, Mahdi Rafiee, Gordon A. Ferns, Habibollah Esmaily, Mahnaz Amini, Tags: Research Communication Source Type: research

Temporal correlation of morphological and biochemical changes with the recruitment of different mechanisms of reactive oxygen species formation during human SW872 cell adipogenic differentiation
In conclusion, the morphological and biochemical changes of differentiating SW872 cells are accompanied by the discontinuous formation of ROS derived from NOX-2, increasingly implicated in adipogenesis and adipose tissue dysfunction. In addition, mROS formation was significant only in the late phase of differentiation and was associated with mitochondrial dysfunction. (Source: BioFactors)
Source: BioFactors - July 15, 2021 Category: Biochemistry Authors: Mara Fiorani, Rita De Matteis, Barbara Canonico, Giulia Blandino, Alessandro Mazzoli, Mariele Montanari, Andrea Guidarelli, Orazio Cantoni Tags: Research Communication Source Type: research

Rapid and simultaneous determination of histidine metabolism intermediates in human and mouse microbiota and biomatrices
AbstractHistidine metabolism is a key pathway physiologically involved in satiety, recognition memory, skin, and neural protection and allergic diseases. Microbiologically-produced imidazole propionate induces type II diabetes and interferes with glucose lowering drugs. Despite their determinant health implications, no single method simultaneously assesses histidine metabolites in urine, feces, and microbiota. The aim of this study was to develop a simple, rapid, and sensitive method for the determination of histidine and its major bioactive metabolites histamine, N-acetylhistamine, imidazole-4-acetate,cis-urocanate,trans-...
Source: BioFactors - July 11, 2021 Category: Biochemistry Authors: Inmaculada Acu ña, Alicia Ruiz, Tomás Cerdó, Samuel Cantarero, Ana López‐Moreno, Margarita Aguilera, Cristina Campoy, Antonio Suárez Tags: Research Communication Source Type: research

Retraction
(Source: BioFactors)
Source: BioFactors - July 4, 2021 Category: Biochemistry Tags: Retraction Source Type: research

The cardioprotective effects of hydrogen sulfide by targeting endoplasmic reticulum stress and the Nrf2 signaling pathway: A review
AbstractCardiac diseases are emerging due to lifestyle, urbanization, and the accelerated aging process. Oxidative stress has been associated with cardiac injury progression through interference with antioxidant strategies and endoplasmic reticulum (ER) function. Hydrogen sulfide (H2S) is generated endogenously froml-cysteine in various tissues including heart tissue. Pharmacological evaluation of H2S has suggested a potential role for H2S against diabetic cardiomyopathy, ischemia/reperfusion injury, myocardial infarction, and cardiotoxicity. Nuclear factor E2-related factor 2 (Nrf2) activity is crucial for cell survival i...
Source: BioFactors - June 24, 2021 Category: Biochemistry Authors: Fatemeh Yarmohammadi, A. Wallace Hayes, Gholamreza Karimi Tags: Review Article Source Type: research

Hsa_circ_0045714 induced by eupatilin has a potential to  promote fracture healing
AbstractIt is thought that maintaining preosteoblast viability is constructive to fracture healing. Here, we explored the effects of eupatilin on preosteoblast and addressed the mechanism associated with hsa_circ_0045714. Blood specimens were collected from 32 patients with hand fracture or calcaneus fracture. MC3T3-E1 cells were treated with eupatilin. Small interfering-RNA was transfected into MC3T3-E1 cells. The ability of MC3T3-E1 cells to survive, proliferate, migrate, and express fracture-associated proteins was examined by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (MTT), 5-bromodeoxyuridine (B...
Source: BioFactors - June 22, 2021 Category: Biochemistry Authors: Yan Sun, Junbo Ge, Weiwei Tang, Huanyu Hong, Dong Liu, Jiangtao Lin Tags: RESEARCH COMMUNICATION Source Type: research

Issue Information
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Source: BioFactors - June 22, 2021 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research

Issue Information ‐ Cover
(Source: BioFactors)
Source: BioFactors - June 22, 2021 Category: Biochemistry Tags: ISSUE INFORMATION ‐ COVER Source Type: research

Issue Highlights
(Source: BioFactors)
Source: BioFactors - June 22, 2021 Category: Biochemistry Tags: ISSUE HIGHLIGHTS Source Type: research

Knockdown of lncRNA TUG1 attenuates cerebral ischemia/reperfusion injury through regulating miR ‐142‐3p
AbstractCerebral ischemia –reperfusion injury (CI/RI) is one of the most common diseases of the central nervous system. At present, there is no specific treatment for CI/RI. It is necessary to explore the mechanism of CI/RI and find new ways to prevent and treat CI/RI. An oxygen and glucose deprivation/recovery (OGD/R) mod el was established to evaluate the effects of mouse astrocytes (MA-C) cell viability and apoptosis of stepwise exposure to oxygen and glucose deprivation followed by their replenishment. This assessment included using taurine upregulated gene 1–small interfering RNAs (TUG1-siRNA) transfection...
Source: BioFactors - June 22, 2021 Category: Biochemistry Authors: Leibing Li, Qi Zhang, Yan Wang, Shixiao Yin, Shaohua Chi, Fei Han, Weijie Wang Tags: Research Communication Source Type: research

Dihydroartemisinin alleviates hepatic fibrosis through inducing ferroptosis in hepatic stellate cells
AbstractTargeting the elimination of activated hepatic stellate cells (HSCs) and blocking excessive deposition of extracellular matrix are recognized as an effective strategy for the treatment of hepatic fibrosis. As a newly discovered programmed cell death mode, the regulatory mechanism of ferroptosis in the clearance of activated HSCs has not been fully elucidated. In the present study, we reported that the induction of ferroptosis in activated HSCs was required for dihydroartemisinin (DHA) to alleviate hepatic fibrosis. Treatment with DHA could improve the damage of hepatic fibrosis in vivo and inhibit the activation of...
Source: BioFactors - June 16, 2021 Category: Biochemistry Authors: Zili Zhang, Xian Wang, Zilong Wang, Zhiyue Zhang, Yashi Cao, Zonghui Wei, Jiangjuan Shao, Anping Chen, Feng Zhang, Shizhong Zheng Tags: Research Communication Source Type: research

Selenium deficiency causes apoptosis through endoplasmic reticulum stress in swine small intestine
This study was to explore whether Se deficiency can cause ER stress and induce apoptosis in swine small intestine. We established the Se deficiency swine model in vivo and the intestinal epithelial (IPEC-J2) cell Se deficiency model in vitro. The results of morphological observation showed that Se deficiency caused structural damage in intestinal villi and the decrease of goblet cell structure. The apoptotic characteristics such as nucleolar condensation, mitochondrial swelling, and apoptotic bodies were observed in the IPEC-J2 cells. The results of acridine orange/ethidium bromide and mitochondrial membrane potential fluo...
Source: BioFactors - June 15, 2021 Category: Biochemistry Authors: Yingying Zheng, Bo Zhang, Haoyue Guan, Xing Jiao, Jie Yang, Jingzeng Cai, Qi Liu, Ziwei Zhang Tags: Research Communication Source Type: research

alpha ‐Tocopherol supplementation reduces inflammation and apoptosis in high cholesterol mediated nonalcoholic steatohepatitis
AbstractInflammation and apoptosis signaling are crucial steps in the progression from nonalcoholic fatty liver disease (NAFLD) to nonalcoholic steatohepatitis (NASH). Alpha-tocopherol, the most active form of vitamin E, is an important modulator of signaling mechanisms, but its involvement to cholesterol-induced NASH pathogenesis remains poorly defined. Herein we have reported a novel effect of α-tocopherol in the transition from hepatic steatosis to NASH. High cholesterol diet alone (without α-tocopherol) in rabbits elevated NASH development as indicated by increased inflammatory response, apoptotic activity ...
Source: BioFactors - June 10, 2021 Category: Biochemistry Authors: Tugce Demirel ‐Yalciner, Erdi Sozen, Esra Ozaltin, Ali Sahin, Nesrin Kartal Ozer Tags: Research Communication Source Type: research

Systemic inflammation and oxidative stress induced by inhaled paraquat in rat improved by carvacrol, possible role of PPAR γ receptors
AbstractControl rats were exposed to saline aerosol, two groups were exposed to paraquat (PQ), 27 (PQ-L) and 54 (PQ-H) mg/m3 aerosols and six groups were treated with carvacrol, 20 (C-L) and 80 (C-H) mg/kg/day, pioglitazone, 5 (Pio-L) and 10 (Pio-H) mg/kg/day, C-L+Pio-L and dexamethasone, 0.03 mg/kg/day, for 16 days after the end of exposure to PQ-H. Different variables were measured after the end of treatment period. Total and differential white blood cells counts, nitrite, malondialdehyde, interleukin (IL)-10, and interferon-gamma levels were significant increased, but thiol, superoxide dismutase, catalase, IL-17, and tu...
Source: BioFactors - June 6, 2021 Category: Biochemistry Authors: Fatemeh Amin, Arghavan Memarzia, Hamideh Kazemi Rad, Farzaneh Shakeri, Mohammad Hossein Boskabady Tags: Research Communication Source Type: research

Cip2A modulates osteogenic differentiation via the  ERK‐Runx2 pathway in MG63 cells
In this study, we found that Cip2A was upregulated under osteogenic conditions in MG63 cells. Besides, overexpression of Cip2A significantly increased the expression of Runt-related transcription factor 2 (Runx2) and alkaline phosphatase (ALP). Inversely, the knockdown of Cip2A in MG63 cells suppressed osteoblast differentiation. Cip2A expression during osteogenic differentiation was mediated by extracellular signal-regulated kinase (ERK) activation. Taken together, our results suggest that Cip2A plays important role in regulating osteoblast differentiation by inducing ERK phosphorylation in MG63 cells. (Source: BioFactors)
Source: BioFactors - June 3, 2021 Category: Biochemistry Authors: Hyo ‐Eun Son, Won‐Gu Jang Tags: Research Communication Source Type: research

Melatonin: A regulator of the interplay between FoxO1, miR96, and miR215 signaling to diminish the growth, survival, and metastasis of murine adenocarcinoma
In conclusion, this study sheds the light on M./P53/miR215/CD44 with an emphasis on M./miR96//foxO1 signaling cascades, as a novel pathway of melatonin signaling in adenocarcinoma to diminish cancer cell growth, survival and metastasis. (Source: BioFactors)
Source: BioFactors - June 1, 2021 Category: Biochemistry Authors: Rehab T. Oshiba, Ehab Touson, Yasser M. Elsherbini, Mohamed E. Abdraboh Tags: Research Communication Source Type: research

Silencing of KIF18B restricts proliferation and invasion and enhances the chemosensitivity of breast cancer via modulating Akt/GSK ‐3β/β‐catenin pathway
AbstractKinesin family member 18B (KIF18B) is a new tumor-associated protein that contributes to the carcinogenesis of multiple malignancies. However, the detailed relevance of KIF18B in breast cancer has not been fully elucidated. This work aimed was to evaluate a possible relationship between KIF18B and breast cancer progression. Our findings show KIF18B is increased in breast cancer and demonstrate that high KIF18B level predicts a reduced survival rate. Cellular functional studies revealed that knockdown of KIF18B markedly reduces the proliferation, invasion, and epithelial –mesenchymal transition of breast cance...
Source: BioFactors - May 31, 2021 Category: Biochemistry Authors: Jue Jiang, Ting Liu, Xin He, Wenqi Ma, Juan Wang, Qi Zhou, Miao Li, Shanshan Yu Tags: Research Communication Source Type: research

Tenascin ‐C regulates migration of SOX10 tendon stem cells via integrin‐α9 for promoting patellar tendon remodeling
In conclusion, TNC regulated the migration of STSCs via ITGA9, thereby promoting the regeneration of tendon injuries. (Source: BioFactors)
Source: BioFactors - May 31, 2021 Category: Biochemistry Authors: Kang Xu, Yibo Shao, Yi Xia, Yuna Qian, Nan Jiang, Xianqiong Liu, Li Yang, Chunli Wang Tags: Research Communication Source Type: research

Zerumbone, a ginger sesquiterpene, inhibits migration, invasion, and metastatic behavior of human malignant glioblastoma multiforme in vitro
AbstractThe most widespread and challenging aggressive malignant tumor in the brain is glioblastoma multiforme (GBM). GBM is characterized, in particular, by significant intratumor cell variability, high growth rates, and widespread invasiveness within the surrounding normal brain parenchyma. The present study aimed to examine the impact of the natural product Zerumbone, a promising sesquiterpenoid phytochemical fromZingiber zerumbet, on U-87 MG GBM cells and its underlying molecular mechanisms. At sub-lethal doses, Zerumbone exerted a concentration- and time-dependent suppression of cell migration ability utilizing scratc...
Source: BioFactors - May 28, 2021 Category: Biochemistry Authors: Mohammad Jalili ‐Nik, Amir R. Afshari, Hamed Sabri, Bahram Bibak, Hamid Mollazadeh, Amirhossein Sahebkar Tags: Research Communication Source Type: research

The HIF ‐1/SNHG1/miR‐199a‐3p/TFAM axis explains tumor angiogenesis and metastasis under hypoxic conditions in breast cancer
AbstractActivation of hypoxia-inducible factors (HIFs) as a result of intratumoral hypoxia modulates a cascade of molecular pathways thus leading to angiogenesis and metastasis in many solid tumors, including breast cancer (BC). In our paper, we report a regulatory axis of HIF-1, SNHG1, miR-199a-3p, and mitochondrial transcription factor A (TFAM) involved in tumor angiogenesis and metastasis under hypoxic conditions in BC. The expression of SNHG1 was determined in human BC cells cultured in hypoxia (1% O2, 24  h) and normoxia (20% O2, 24  h). Cultured MDA-MB-231 cells were assayed for the proliferation, migra...
Source: BioFactors - May 19, 2021 Category: Biochemistry Authors: Yonggang Zuo, Changping Qu, Yanyan Tian, Yuqing Wen, Shuguan Xia, Mingde Ma Tags: Research Communication Source Type: research

Antioxidant and reducing lipid accumulation effects of rutin in Caenorhabditis elegans
In this study, the effect of rutin on fatty acid metabolism and antioxidant activity were evaluated. We found that the antioxidant capacity of rutin-treatedCaenorhabditis elegans was enhanced but the triglyceride content was significantly reduced. The reduction of fat accumulation by rutin was also confirmed by Oil Red O staining. RNA-seq analysis indicated that rutin significantly regulated the expression of seven genes related to lipid metabolism inC. elegans. Among the seven genes,acox-1.3,stdh-3, andfat-7 were associated with fatty acid metabolism. Rutin significantly reduced fat accumulation in bothfat-6 andfat-7 muta...
Source: BioFactors - May 14, 2021 Category: Biochemistry Authors: Xianjin Qin, Wenqian Wang, Weihua Chu Tags: Research Communication Source Type: research

Interleukin ‐9 deficiency affects lipopolysaccharide‐induced macrophage‐related oxidative stress and myocardial cell apoptosis via the Nrf2 pathway both in vivo and in vitro
This study aimed to investigate the role of IL-9 in lipopolysaccharide (LPS)-induced myocardial cell (MC) apoptosis. Mice were treated with LPS, and IL-9 expression was measured and the results showed that compared with WT mice, LPS-treated mice exhibited increased cardiac M ø-derived IL-9. Additionally, the effects of IL-9 deficiency (IL-9−/−) on macrophage (Mø)-related oxidative stress and MC apoptosis were evaluated, the results showed that IL-9 knockout significantly exacerbated cardiac dysfunction, inhibited Nrf2 nuclear transfer, promoted an imbalance in M1 and M2 Møs, and exacerbated...
Source: BioFactors - May 12, 2021 Category: Biochemistry Authors: Zhishan Liang, Fuze Pan, Zicong Yang, Mengjie Wang, Changxing Hu, Lei Shi, Qingwei Ji, Ling Liu Tags: Research Communication Source Type: research

Bioinformatics and experimental findings reveal the therapeutic actions and targets of pachymic acid against cystitis glandularis
In this study, we aimed to apply the network pharmacology and molecular docking analyses to reveal concrete anti‐CG targets an d mechanisms of PA, and then the bioinformatic findings were verified by using clinical and animal samples. The methodological data from network pharmacology approach showed that 303 and 243 reporting targets of CG and PA, and other 31 shared targets of CG and PA were identified. Subsequently, all t op targets of PA against CG were screened out, including cyclooxygenase‐2, epidermal growth factor receptor, tumor antigen p53 (TP53), tumor necrosis factor‐alpha (TNF), interleukin‐1 (IL‐1) b...
Source: BioFactors - May 4, 2021 Category: Biochemistry Authors: Zihao Feng, Hailin Shi, Bojian Liang, Tianyu Ge, Menghui Cai, Feng Liu, Kunping Huang, Jintao Wen, Qiuhong Chen, Bo Ge Tags: Research Communication Source Type: research

Curcumin: A small molecule with big functionality against amyloid aggregation in neurodegenerative diseases and type 2 diabetes
AbstractAmyloidosis is a concept that implicates disorders and complications that are due to abnormal protein accumulation in different cells and tissues. Protein aggregation ‐associated diseases are classified according to the type of aggregates and deposition sites, such as neurodegenerative disorders and type 2 diabetes mellitus. Polyphenolic phytochemicals such as curcumin and its derivatives have anti‐amyloid effects both in vitro and in animal models; however, the underlying mechanisms are not understood. In this review, we summarized possible mechanisms by which curcumin could interfere with self‐assembly proc...
Source: BioFactors - April 24, 2021 Category: Biochemistry Authors: Shabnam Radbakhsh, George E. Barreto, Abigail R. Bland, Amirhossein Sahebkar Tags: Review Article Source Type: research

Secondary CoQ10 deficiency, bioenergetics unbalance in disease and aging
AbstractCoenzyme Q10 (CoQ10) deficiency is a rare disease characterized by a decreased accumulation of CoQ10 in cell membranes. Considering that CoQ10 synthesis and most of its functions are carried out in mitochondria, CoQ10 deficiency cases are usually considered a mitochondrial disease. A relevant feature of CoQ10 deficiency is that it is the only mitochondrial disease with a successful therapy available, the CoQ10 supplementation. Defects in components of the synthesis machinery caused by mutations inCOQ genes generate the primary deficiency of CoQ10. Mutations in genes that are not directly related to the synthesis ma...
Source: BioFactors - April 20, 2021 Category: Biochemistry Authors: Pl ácido Navas, María V. Cascajo, María Alcázar‐Fabra, Juan D. Hernández‐Camacho, Ana Sánchez‐Cuesta, Ana Belén Cortés Rodríguez, Manuel Ballesteros‐Simarro, Antonio Arroyo‐Luque, Juan Carlos Rodríguez‐Aguilera, Daniel J. M. Tags: Review Article Source Type: research

Long ‐COVID syndrome‐associated brain fog and chemofog: Luteolin to the rescue
AbstractCOVID ‐19 leads to severe respiratory problems, but also to long‐COVID syndrome associated primarily with cognitive dysfunction and fatigue. Long‐COVID syndrome symptoms, especially brain fog, are similar to those experienced by patients undertaking or following chemotherapy for cancer (chemofog or chemobrain), as well in patients with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) or mast cell activation syndrome (MCAS). The pathogenesis of brain fog in these illnesses is presently unknown but may involve neuroinflammation via mast cells stimulated by pathogenic and stress stimul i to release me...
Source: BioFactors - April 20, 2021 Category: Biochemistry Authors: Theoharis C. Theoharides, Christos Cholevas, Konstantinos Polyzoidis, Antonios Politis Tags: REVIEW ARTICLE Source Type: research

Issue Information
(Source: BioFactors)
Source: BioFactors - April 20, 2021 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research

Issue Information ‐ Cover
(Source: BioFactors)
Source: BioFactors - April 20, 2021 Category: Biochemistry Tags: ISSUE INFORMATION ‐ COVER Source Type: research

Luteolin: The wonder flavonoid
(Source: BioFactors)
Source: BioFactors - April 20, 2021 Category: Biochemistry Authors: Theoharis C. Theoharides Tags: PREFACE Source Type: research

Long ‐COVID syndrome‐associated brain fog and chemofog: Luteolin to the rescue
AbstractCOVID ‐19 leads to severe respiratory problems, but also to long‐COVID syndrome associated primarily with cognitive dysfunction and fatigue. Long‐COVID syndrome symptoms, especially brain fog, are similar to those experienced by patients undertaking or following chemotherapy for cancer (chemofog or chemobrain), as well in patients with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) or mast cell activation syndrome (MCAS). The pathogenesis of brain fog in these illnesses is presently unknown but may involve neuroinflammation via mast cells stimulated by pathogenic and stress stimul i to release me...
Source: BioFactors - April 20, 2021 Category: Biochemistry Authors: Theoharis C. Theoharides, Christos Cholevas, Konstantinos Polyzoidis, Antonios Politis Tags: REVIEW ARTICLE Source Type: research

Issue Information
(Source: BioFactors)
Source: BioFactors - April 20, 2021 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research

Issue Information ‐ Cover
(Source: BioFactors)
Source: BioFactors - April 20, 2021 Category: Biochemistry Tags: ISSUE INFORMATION ‐ COVER Source Type: research

Luteolin: The wonder flavonoid
(Source: BioFactors)
Source: BioFactors - April 20, 2021 Category: Biochemistry Authors: Theoharis C. Theoharides Tags: PREFACE Source Type: research

The unique molecular targets associated antioxidant and antifibrotic activity of curcumin in in vitro model of acute lung injury: A proteomic approach
This study generated molecular evidence to deepen our understanding of the therapeutic role of curcumin at the proteomic level and may be useful to identify molecular targets for future drug discovery. (Source: BioFactors)
Source: BioFactors - April 17, 2021 Category: Biochemistry Authors: Sadiya Bi Shaikh, Mohd Altaf Najar, Ashwini Prabhu, D. A. B. Rex, Jaikanth Chanderasekaran, Santosh Kumar Behera, Prashant Kumar Modi, Thottethodi Subrahmanya Keshava Prasad, Yashodhar Prabhakar Bhandary Tags: Research Communication Source Type: research

Time ‐based investigation of urinary metabolic markers for Type 2 diabetes: Metabolomics approach for diabetes management
The objective of this study was to monitor the time ‐course variation in the metabolic profile of diabetic rats to detect urinary metabolic biomarkers using the metabolomics approach. Type 2 diabetes was induced in male Wistar albino rats using a single intraperitoneal injection of 40 mg/kg of streptozotocin following oral administration of 10% f ructose in drinking water for 3 weeks. Then, urine was collected for 24 h from rats at three time points (0, 2, and 4 weeks after confirmation of diabetes), and were analyzed by nuclear magnetic resonance (H1‐NMR), followed by multivariate data anal...
Source: BioFactors - April 9, 2021 Category: Biochemistry Authors: Moataz M. Hanafy, Jeremie Z. Lindeque, Shohda A. El ‐Maraghy, Abdel‐Hamid Z. Abdel‐Hamid, Nancy N. Shahin Tags: Research Communication Source Type: research