Allogeneic Hematopoietic Stem Cell Transplantation in Therapy Related Acute Leukemia
AbstractTherapy related acute leukemia consists of a unique subset of acute leukemia with an increased frequency of high risk cytogenetic and molecular abnormalities, dismal response to therapy, higher relapse rates and poor overall survival. Therapy related acute myeloid leukemia (t-AML) is a better defined disease entity than therapy related acute lymphoid leukemia (t-ALL). However, in recent times, t-ALL is also being increasingly recognized and extensively studied. Therapy related acute myeloid leukemia is usually classified together with therapy related myelodysplastic syndrome. However, the management of these two di...
Source: Indian Journal of Hematology and Blood Transfusion - August 12, 2020 Category: Hematology Source Type: research

The Treatment Effect of Protamine on Severe Coagulopathy in Epstein –Barr Virus-Associated Hemophagocytic Lymphohistiocytosis: Case Reports and Literature Review
AbstractHemophagocytic lymphohistiocytosis (HLH) is a rare and life-threatening disease, which is characterized by severe systemic inflammation with cytokine storm as well as histologic evidence of hemophagocytosis. Besides, coagulopathy and hemorrhages are two common severe complications in HLH patients. Recent literatures indicate that Epstein –Barr virus (EBV) infection is one of the important triggers for the disease. In the study, we present three cases of EBV related HLH (EBV-HLH) with coagulopathy in patients with distinct backgrounds. Case 1 is a 45-year-old female diagnosed with EBV associated NK/T cell lymp...
Source: Indian Journal of Hematology and Blood Transfusion - August 12, 2020 Category: Hematology Source Type: research

Hemostatic Abnormalities in COVID-19: An Update
AbstractCOVID-19 has emerged as a pandemic with lung being the primarily afflicted organ. Deranged hemostasis has been observed in patients with COVID-19 with scales tipped towards a prothrombotic state. The pathogenesis differs from disseminated intravascular coagulation with a primary pulmonary localization. This is referred to as pulmonary intravascular coagulopathy with strong component of thrombo-inflammation. This is reflected in the lab tests with an increase in D-dimer which correlates with severity and outcomes of disease. Common coagulation tests such as prothrombin time, activated partial thromboplastin time are...
Source: Indian Journal of Hematology and Blood Transfusion - August 11, 2020 Category: Hematology Source Type: research

Hb Brugg [ HBA1 :c.63C & gt;A]: Report of an Ultra-Rare Variant Hemoglobin and Its Co-inheritance with Hb D-Punjab
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - August 10, 2020 Category: Hematology Source Type: research

Primary Bone Lymphoma: A 13 Year Retrospective Institutional Analysis in the Chemo-Immunotherapy Era
AbstractPrimary Lymphoma of bone (PBL) is an uncommon extranodal tumor accounting for 1% of all malignant lymphomas. The incidence of PBL is so rare that many of its aspects remain unknown. We retrospectively analysed our data in order to know clinical characteristics and treatment outcome in Indian population in chemo-immunotherapy era. We identified 49 patients [2007 –2019] (median age 52 years) of which, 35 (71.4%) were males. Nearly one-third patients (n = 18; 36.8%) were elderly (Age >  60). The most common histological subtype was DLBCL. Local pain /swelling (n =&thins...
Source: Indian Journal of Hematology and Blood Transfusion - August 8, 2020 Category: Hematology Source Type: research

Clinical Profile and Severity of Hemolysis in Adult Patients of Primary Autoimmune Hemolytic Anemia and Their Response to Steroid: A Prospective Cohort Study from Single Institution
This study on patients of adult primary AIHA was conducted by taking complete history followed by detail physical examination. Laboratory investigations were performed to establish haemolytic anaemia and to assess severity of haemolysis. Immunehematological work up including blood grouping, direct antiglobulin test (DAT), IAT, antibody screening, adsorption elution was performed to diagnose type of AIHA. All cases were followed up to assess the response to prednisolone. All the data were collected and analysed by SPSS 19. Out of 62 primary AIHA cases, female were affected more than male (41:21). WAIHA is most common type (...
Source: Indian Journal of Hematology and Blood Transfusion - August 7, 2020 Category: Hematology Source Type: research

Clinico-Pathologic Profile and Treatment Outcomes of Patients with Diffuse Large B Cell Lymphoma Based on Cell of Origin Classification
AbstractDiffuse large B cell lymphoma (DLBCLs) constitute 40% of all non-Hodgkin lymphoma and it represent a heterogeneous group of neoplasms rather than a single clinicopathological entity. We analysed the outcomes and clinical features based on the cell of origin in a series of patients with DLBCL from our institute. Medical case records of all newly diagnosed DLBCL treated in our institute from January 2015 to July 2017 were analysed for this study. Cell of origin classification was based on immunohistochemistry using Hans algorithm. Kaplan –Meier curves were used to determine survival. Ninety-five patients were d...
Source: Indian Journal of Hematology and Blood Transfusion - July 27, 2020 Category: Hematology Source Type: research

Non Malignant Lymphoproliferative Disorders in Children: A Case Series
We describe series of cases with non malignant LPD encountered in our practice and their varied clinical presentation, difficulties in diagnosis, underlying etiology, treatment and outcome. Many of these disorders are self limiting, however some are associated with significant morbidity, hence treatment must be tailored based on the underlying immune dysfunction and aggressiveness of the clone. (Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - July 27, 2020 Category: Hematology Source Type: research

Downregulation of MIR100HG Induces Apoptosis in Human Megakaryoblastic Leukemia Cells
AbstractLong noncoding ribonucleic acids (lncRNAs) are ribonucleic acid (RNA) molecules longer than 200 nucleotides without protein-coding capacity. Several studies have shown that lncRNAs play a pivotal role in the initiation, maintenance, and progression of acute myeloid leukemia (AML), which could make them a promising candidate in the diagnosis and treatment of leukemia. Acute Megakaryoblastic leukemia (AMKL) is a rare form of AML with a poor prognosis and low survival. It has been reported that lncRNA MIR100HG is involved several types of malignancies. In the present study, MIR100HG was downregulated in a human acute ...
Source: Indian Journal of Hematology and Blood Transfusion - July 24, 2020 Category: Hematology Source Type: research

A Comparative Study of Bone Marrow Squash and Wedge Aspiration Smears
This study was conducted over a period of one year. Two hundred and five bone marrow aspirates were included. Both squash and wedge smears were made. Blinded slide review was done. Bone marrow cellularity, megakaryocyte number, myeloid to erythroid (M:E) ratio, morphology and final diagnosis on each smear type was compared. Chi square test, t-test and Kappa were applied to study the agreement between the wedge and squash smears. Among the 205 patients studied, squash smears showed significant over estimation of cellularity and megakaryocyte number (p   0.05) in the M:E ratio and morphological scores. Most pat...
Source: Indian Journal of Hematology and Blood Transfusion - July 20, 2020 Category: Hematology Source Type: research

The Journey of Primary Myelofibrosis to Splenic Marginal Zone Lymphoma on the Wheels of Ruxolitinib
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - July 16, 2020 Category: Hematology Source Type: research

Unorthodox Flowcytometric Scatter and Severe Bone Marrow Fibrosis in De Novo Acute Promyelocytic Leukaemia
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - July 12, 2020 Category: Hematology Source Type: research

Clinical and Laboratory Profiles of 17 Cases of Chronic Granulomatous Disease in North India
AbstractA primary immune deficiency disorder is often suspected in children with recurrent deep seated and fungal infections and those admitted to pediatric  intensive care units. Chronic granulomatous disease (CGD) is inherited disorder leading to infections caused due to defective superoxide production. Cases referred for testing for a primary immunodeficiency disorder were tested for Dihydrorhodamine 123 (DHR) assay by flow cytometry and nitroblue t etrazolium dye (NBT) slide test. The unstimulated and stimulated samples were tested for oxidative burst activity which gives bright fluorescence due to formation of Rh...
Source: Indian Journal of Hematology and Blood Transfusion - July 9, 2020 Category: Hematology Source Type: research

Conditioning Regimens for Relapsed/Refractory Lymphoma Patients Undergoing Autologous Stem Cell Transplantation: BEAM Versus High Dose ICE
AbstractThere are different drug combinations and conditioning regimens in lymphoma transplants. However, no randomized data is available to demonstrate the superiority of any regimen and the optimal choice is unknown. In this analysis, we compared the efficacy, toxicity and the survival outcomes of the BEAM and the high dose ICE (hdICE) conditioning regimens in relapsed NHL and relapsed/refractory Hodgkin Lymphoma patients undergoing auto-SCT. 83 patients with relapsed/refractory HL or relapsed NHL who were treated with Auto-SCT between 2006 and 2016, were analyzed retrospectively. 52 patients (62.7%) received BEAM, while...
Source: Indian Journal of Hematology and Blood Transfusion - July 8, 2020 Category: Hematology Source Type: research

COVID-19 in Adult Patients with Hematological Disease: Analysis of Clinical Characteristics and Outcomes
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - July 7, 2020 Category: Hematology Source Type: research

Blastic Plasmacytoid Dendritic Cell Neoplasm: Single Center Experience on a Rare Hematological Malignancy
ConclusionIntensive induction therapies followed by AHSCT in CR seems to be best approaches for patients with BPDCN. Thus, more effective treatment strategies particularly targeted therapies should be warranted to improve the survival of patients with this rare disease. (Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - July 4, 2020 Category: Hematology Source Type: research

Orbital Rhabdomyosarcoma Infiltrating the Bone Marrow: A Potential Mimic of a Haematopoietic Neoplasm
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - July 3, 2020 Category: Hematology Source Type: research

Sequential Treatment of Arsenic Trioxide Followed by All Trans Retinoic Acid with Anthracyclines has Excellent Long-Term Cure in Acute Promyelocytic Leukemia
AbstractAcute promyelocytic leukemia (APL) remains the most curable myeloid leukemia made feasible through effective use of two differentiating agents, all trans retinoic acid (ATRA) and arsenic trioxide (ATO) with or without chemotherapy (CT). However, early morbidity and mortality remains a problem. With the objective of reducing early death a strategy of sequential induction ATO followed by consolidation ATRA in combination with CT was adopted by our group. The long-term outcomes of patient of APL treated on this sequential approach at our center was analyzed. In this retrospective analysis of prospectively maintained d...
Source: Indian Journal of Hematology and Blood Transfusion - June 26, 2020 Category: Hematology Source Type: research

Comparison of Clinical Features Between Primary and Secondary Breast Diffuse Large B Cell Lymphoma: A Yokohama Cooperative Study Group for Hematology Multicenter Retrospective Study
AbstractWe performed a retrospective analysis of DLBCL with breast involvement to compare the prognosis of primary breast lymphoma (PBL) to secondary breast lymphoma (SBL; especially in limited stage cases). We retrospectively reviewed records of 25 diffuse large B-cell lymphoma (DLBCL) patients with breast involvement who received chemotherapy between January 2000 and August 2012. We compared clinical features and prognosis among patients with PBL (n = 11), limited stage SBL (LSBL;n = 6), and advanced stage SBL (ASBL,n = 8). The PBL group had significantly lesser patients with bre...
Source: Indian Journal of Hematology and Blood Transfusion - June 23, 2020 Category: Hematology Source Type: research

The Impact of Thromboelastography on Blood Transfusion Policy in Adult Cardiac Surgery —A Retrospective Observational Study from Eastern India
AbstractThis  study was conducted to determine the impact of thromboelastography (TEG) on blood transfusion policy regarding utilization and preparation of cryoprecipitate in adult cardiac surgery. The differences in total transfusion requirement, length of postoperative ICU stay and 24 h mortality were also studied after introduction of TEG in transfusion protocol. It was a retrospective, single-center, observational study conducted in adult patients underwent cardiac surgery from April 2008 to March 2016. Two thousand patients underwent surgery when TEG was used compared with 1000 control patients bef ore&...
Source: Indian Journal of Hematology and Blood Transfusion - June 23, 2020 Category: Hematology Source Type: research

Intravenous Fat Emulsion Does Not Significantly Alter Clotting Markers in Dabigatran-Treated Blood
AbstractDabigatran etexilate is an oral direct thrombin (Factor IIa) inhibitor approved for patients with atrial fibrillation and for management of risk of deep vein thrombosis and pulmonary embolism. Dabigatran offers advantages over treatment with warfarin, including limited laboratory monitoring. It is equivalent in prevention of stroke and deep vein thrombosis with essentially equivalent complication rates. In contrast to warfarin, reversal of the anticoagulation is less well established. Idarucizumab is available for reversal, however supporting research is mixed; the agent also happens to be quite expensive making av...
Source: Indian Journal of Hematology and Blood Transfusion - June 22, 2020 Category: Hematology Source Type: research

The Association of Pre-diagnostic Inflammatory Markers and Adipokines and the Risk of Non-Hodgkin Lymphoma Development in Egypt
AbstractNon-Hodgkin lymphoma (NHL) is an etiologically, clinically, and histologically heterogeneous group of lymphoproliferative disorders. Immune dysfunction is a well-known risk factor and dysregulation of cytokines may mediate disease progression. Obesity is one of the important relations connecting immune system abnormalities and lymphomagenesis. We conducted a study to find out the association between obesity as measured by body mass index (BMI), and risk of non-Hodgkin lymphoma (NHL) development by assessment the of inflammatory cytokines levels, (IL-6, IL-10, IFN-gamma and CRP) and adipokines levels (leptin and adi...
Source: Indian Journal of Hematology and Blood Transfusion - June 19, 2020 Category: Hematology Source Type: research

Comparison of Two High-Pressure Liquid Chromatography Instruments Bio-Rad Variant-II and Tosoh HLC-723G11 in the Evaluation of Hemoglobinopathies
AbstractHemoglobin High-performance liquid chromatography (Hb HPLC) is a standard first-line technique for diagnosis of thalassemia and hemoglobinopathies. We compared two HPLC systems for detection and quantification of normal and abnormal Hb fractions. EDTA samples from 100 normal healthy subjects and 107 subjects affected with hemoglobinopathies or carriers were analysed using HPLC systems Tosoh HLC-723G11 and Bio-Rad Variant-II. Retention time (RT) and area of peaks for HbA2, HbF and other structural variants were compared. In discrepant cases samples were run on Sebia Capillary zone electrophoresis (CZE) for confirmat...
Source: Indian Journal of Hematology and Blood Transfusion - June 19, 2020 Category: Hematology Source Type: research

Clinical Course of Patients With Sickle Cell Anemia and Co-inherited Hematological Disorders: Experience at a Tertiary Hematological Centre
In conclusion, HbA reduces severity of HbS in HbS-β + thalassemia. HbS-HbD dis ease can manifest as a transfusion dependent illness. HbSS reduces severity of G6PD deficiency after first few years of life. HbSS and hemophilia coinheritance ameliorates symptoms of hemophilia. (Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - June 12, 2020 Category: Hematology Source Type: research

Clinico-Hematological Profile of Paroxysmal Nocturnal Hemoglobinuria in Indian Patients: FLAER Flow Cytometry Based Experience from an Indian Tertiary Care Centre
AbstractPNH is a rare disease with wide spectrum of intra-vascular hemolysis and thrombosis to sub-clinical PNH clones. We aimed to study the clinico-hematological profile and clone size on granulocytes and monocytes of PNH patients classified as per International PNH Interest Group recommendations. A retrospective analysis of clinico-hematological profile of 112 PNH clone positive patients by FLAER based flow cytometry between January and September 2017 done and classified into classical PNH, PNH with aplastic anemia or myelodysplastic syndrome (PNH-AA/MDS) and sub-clinical PNH clones (PNH-sc). Of 112 patients, majority w...
Source: Indian Journal of Hematology and Blood Transfusion - June 10, 2020 Category: Hematology Source Type: research

Inherited Bleeding Disorders in Pediatric Patients; experience of the national referral center in Iraq
AbstractBackgroundInherited bleeding disorders can lead to lifelong bleeding; they are mainly caused by quantitative or qualitative defect of coagulation factors, von Willebrand factor (VWF) or platelets. No published data are available about the different types of inherited bleeding disorders in Iraq.ObjectivesTo describe types, severity and presentation of inherited bleeding disorders in pediatric patients in the major referral center in Iraq.Patients and MethodsThis is a cohort prospective descriptive study conducted at the National Center of Hematology, a referral center for bleeding disorders in Baghdad-Iraq, from Jan...
Source: Indian Journal of Hematology and Blood Transfusion - June 10, 2020 Category: Hematology Source Type: research

A Case of Severe Hemolytic Disease of the Newborn Caused by Anti-Di a and SLC4A1 Gene Analysis in the Family
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - June 10, 2020 Category: Hematology Source Type: research

Safety and Efficacy of Bendamustine-Rituximab in Treatment Na ïve Symptomatic Follicular Lymphoma: An Institutional Analysis
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - June 9, 2020 Category: Hematology Source Type: research

AML-MRC with Basophilia Mimicking CLPD Due to Abnormally Clumped Chromatin
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - June 8, 2020 Category: Hematology Source Type: research

Pseudo-Thrombocytosis Caused by Extreme Microcytosis in a Patient with Alpha Thalassemia Trait
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - June 8, 2020 Category: Hematology Source Type: research

How to Use a Prioritised Approach for Treating Hematological Disorders During the COVID-19 Pandemic in India?
AbstractThe current pandemic coronavirus, SARS-CoV-2, is known to cause severe infection (COVID-19) in patients with comorbidities, particularly cancer or an immunosuppressed state. Most healthcare systems in the country are likely to be overwhelmed soon if the pandemic moves to a stage of community transmission. Currently, limited evidence is available for managing patients with hematological disorders during the COVID-19 pandemic. The current review summarises the possible challenges clinicians are likely to face, key considerations to guide decision making, and possible solutions to the anticipated challenges. Disease s...
Source: Indian Journal of Hematology and Blood Transfusion - June 6, 2020 Category: Hematology Source Type: research

Morphology As a Guide to the Diagnosis of a Rare Cause of Neutropenia
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - June 5, 2020 Category: Hematology Source Type: research

Primary Mediastinal B Cell Lymphoma: A Limited Institutional Experience with Uniform DAEPOCH-R Protocol
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - June 5, 2020 Category: Hematology Source Type: research

Does Development of Plasmacytosis have a Role in Spontaneous Remission of Acute Myeloid Leukemia?
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - June 4, 2020 Category: Hematology Source Type: research

Recent Advances in Molecular Diagnosis and Prognosis of Childhood B Cell Lineage Acute Lymphoblastic Leukemia (B-ALL)
AbstractB cell lineage acute lymphoblastic leukemia is the most common leukemia occurring in children and young adults and is the leading cause of cancer related deaths. The 5  year overall survival outcome in children with B-ALL has improved significantly in the last few decades. In the past, the discovery of various genetic alterations and targeted therapy have played a major role in decreasing disease-related deaths. In addition, numerous advances in the pathogenesis of B-ALL have been found which have provided better understanding of the genes involved in disease biology with respect to diagnostic and prognostic i...
Source: Indian Journal of Hematology and Blood Transfusion - June 3, 2020 Category: Hematology Source Type: research

Successful Treatment of Acquired Hemophilia A with Bortezomib Plus Low Dose Rituximab in a Patient with Renal Failure and Severe Pneumonia
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - June 3, 2020 Category: Hematology Source Type: research

Impact of ABCB1 Gene (C3435T/A2677G) Polymorphic Sequence Variations on the Outcome of Patients with Chronic Myeloid Leukemia and Acute Lymphoblastic Leukemia in Kashmiri Population: A Case –Control Study
AbstractInherited polymorphic sequence variations in drug transport genes likeABCB1 impact a portion of patients with hematologic malignancies that show intrinsic or acquire resistance to treatment. Keeping in view inter-individual sensitivities for such drugs, we through this case –control study tested whetherABCB1 C3435T and G2677T polymorphisms have any influence on the risk and treatment response in patients with chronic myeloid leukemia (CML) and B-acute lymphoblastic leukemia (B-ALL). Genotyping forABCB1 polymorphisms was performed by polymerase chain reaction-restriction fragment length polymorphism in 100 CML...
Source: Indian Journal of Hematology and Blood Transfusion - May 26, 2020 Category: Hematology Source Type: research

JAK2V617F Mutation in Patient with Splanchnic Vein Thrombosis
AbstractSplanchnic vein thrombosis is an uncommon life-threatening form of venous thrombosis. It is one the common complication among MPN ’s. In the western studies the prevalence of JAK2V617F mutation among SVT patient is high and ranges from 7 to 59%. The frequency of this mutation among Indian SVT patients is heterogenous. This was a prospective case control study. A total 52 cases of SVT and 40 controls were screened for JAK2V61 7F mutation along with other routine thrombophilic risk factors. Out of total 52 cases, 10 had BCS, 2 had MVT and rest 40 were of PVT/EHPVO. The JAK2V617F mutation was seen in two cases a...
Source: Indian Journal of Hematology and Blood Transfusion - May 25, 2020 Category: Hematology Source Type: research

Study of Prothrombotic Changes in Metabolic Syndrome
AbstractThe metabolic syndrome is a complex disorder of various metabolic risk factors in a single individual having central obesity and commonly associated with diabetes and cardiovascular diseases. The aim of our study was to study the relationship between coagulation abnormalities and metabolic syndrome. We performed a prospective cross-sectional study in a tertiary care hospital. A total of fifty cases of metabolic syndrome and fifty age& sex matched controls were selected. These two groups were investigated for Prothrombin Time (PT), Activated Partial Thromboplastin Time (APTT), Fibrinogen levels, Plasminogen Acti...
Source: Indian Journal of Hematology and Blood Transfusion - May 20, 2020 Category: Hematology Source Type: research

A Novel PDGFR β Rearrangement in Systemic Mastocytosis with an Associated Haematologic Neoplasm
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - May 12, 2020 Category: Hematology Source Type: research

Dapsone: An Old but Effective Therapy in Pediatric Refractory Immune Thrombocytopenia
AbstractThere are no definitive guidelines for management of chronic or refractory immune thrombocytopenia (ITP) in children. Dapsone is an inexpensive and efficacious, yet neglected, therapeutic option for treatment of chronic ITP. We evaluated the efficacy and safety of dapsone in the management of chronic ITP in children. Children with chronic ITP  
Source: Indian Journal of Hematology and Blood Transfusion - May 7, 2020 Category: Hematology Source Type: research

Alternative Strategies in Thalassemia: Focus on Thalidomide
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - May 5, 2020 Category: Hematology Source Type: research

Comparison of Bone Marrow Involvement with Bone Marrow Biopsy and PET –CT and Evaluation of Any Effects on Survival in Patients Diagnosed with Hodgkin and Non-Hodgkin Lymphoma
AbstractWe aimed to demonstrate whether PET –CT can replace bone marrow biopsy in detecting bone marrow involvement in subtypes of lymphoma. In addition, we aimed to also reveal whether there is a difference between the mean survival of patients with bone marrow involvement via PET–CT or biopsy. A total of 276 newly diagnosed lymphoma pat ients who underwent bone marrow biopsy and PET–CT prior to the treatment were scanned retrospectively. Bone marrow biopsy was used as the standard method to investigate the presence of bone marrow involvement in PET–CT. The relationship between bone marrow involvem...
Source: Indian Journal of Hematology and Blood Transfusion - May 4, 2020 Category: Hematology Source Type: research

Role of Hematological Indices as a Screening Tool of Beta Thalassemia Trait in Eastern Uttar Pradesh: An Institutional Study
AbstractA large majority of microcytic hypochromic anemia have defects in cellular hemoglobin synthesis due to either iron deficiency or thalassemia trait; both differing in management and prognosis. HPLC and serum iron profile as confirmatory tests are unavailable at health care centers. Blanket therapy of iron supplements is therefore given in all such cases which may cause iron overload in thalassemia cases. Easy to use and cost effective screening methods are desirable. The present study was undertaken to evaluate the diagnostic accuracy of twelve indices to effectively screen cases of thalassemia trait and differentia...
Source: Indian Journal of Hematology and Blood Transfusion - May 2, 2020 Category: Hematology Source Type: research

Kidd Blood Group Genotyping for Thalassemia Patient in Iran
AbstractWe aimed to determine the JK genotype in thalassemia patients from Iran using different molecular methods to compare with phenotyping results. We also aimed to standardize for the first time, the Tetra-Primer ARMS PCR method for JK genotyping. The serology method cannot correctly determine the phenotype of blood group antigens in patients with multiple blood transfusions. Peripheral blood samples were taken from two hundred alloimmunized thalassemic patients in Tehran Adult Thalassemic Clinic. The samples were tested phenotypically by routine serological methods. After DNA Extraction, SSP-PCR was performed. DNA seq...
Source: Indian Journal of Hematology and Blood Transfusion - April 29, 2020 Category: Hematology Source Type: research

Association of Interleukin-1 β-31C/T, -511T/C and -3954C/T Single Nucleotide Polymorphism and Their Blood Plasma Level in Acquired Aplastic Anemia
AbstractAplastic anemia (AA) is an immune-mediated disorder in which hematopoietic stem and progenitor cells are targeted by a number of cellular and molecular pathways. This case control study aims to investigate the association of interleukin-1beta (IL-1 β) gene polymorphisms, (IL-1 β-31, IL-1β-511and IL-1 β-3954) and their plasma levels with acquired AA. Genotyping was done by Restricted Fragment Length Polymorphism (PCR –RFLP) method andIL-1 β plasma levels were evaluated in peripheral blood using ELISA. Increased level ofIL-1 β was reported to be significant in cases as compared to ...
Source: Indian Journal of Hematology and Blood Transfusion - April 26, 2020 Category: Hematology Source Type: research

Immune Thrombocytopenia: An Atlantic Divide in Nomenclature?
(Source: Indian Journal of Hematology and Blood Transfusion)
Source: Indian Journal of Hematology and Blood Transfusion - April 17, 2020 Category: Hematology Source Type: research

The Efficacy of Cladribine (2-CdA) in Advanced Systemic Mastocytosis
AbstractSystemic mastocytosis (SM) is a rare clonal disorder with multi-organ involvements and shortened life expectancy. To date, no curative treatment for SM exists. Cladribine (2-CdA) is a purine analogue showing activity against neoplastic mast cells and its use was found to be effective in some patients with SM. Nine patients (six males and three females) with advanced SM at median age of 63  years (range 33–67) who received at least one course of 2-CdA were included in a retrospective analysis. Study patients were classified as having aggressive SM (ASM;n = 7) and SM with an associated hemato...
Source: Indian Journal of Hematology and Blood Transfusion - April 15, 2020 Category: Hematology Source Type: research

The Prognostic Impact of Epidermal Growth Factor Receptor (EGFR) in Patients with Acute Myeloid Leukaemia
AbstractExpression of Epidermal Growth Factor Receptor (EGFR), an important proto-oncogene, regulates cell differentiation, proliferation, cell migration and survival in most of the cancer types.EGFR expression has been reported in Acute Myeloid Leukaemia (AML), however, many other reports nullifiedEGFR expression in AML. These contradictory data prompted us to reevaluate the expression ofEGFR in AML and carry out a comparative survival analysis betweenEGFR expressing and non-expressing AML patients (Children and Acute Promyelocytic Leukaemia patients excluded). Bone marrow and/or peripheral blood samples were collected fr...
Source: Indian Journal of Hematology and Blood Transfusion - April 9, 2020 Category: Hematology Source Type: research

Application of Sysmex XN-Series Automated Haematology Analyser in the Rapid Detection of Malaria
AbstractMalaria, an important parasitic disease worldwide, still has diagnostic challenges in the laboratory. Many studies have been conducted on the detection ability of haematology analysers for malaria. We evaluated the Sysmex XN-series analyser as a tool for detection of malaria by analysing the leukocyte cell population data (LCPD), scattergrams and associated Flow Cytometry Standard (FCS) data from both the WNR (white cell nucleated) and WDF (white cell differential) channels. 1281 clinically suspected cases of malaria were screened for malaria by peripheral blood smear examination and were run in the Sysmex XN-1000 ...
Source: Indian Journal of Hematology and Blood Transfusion - April 3, 2020 Category: Hematology Source Type: research